CN104435632A - Traditional Chinese medicine prescription for treating red-neck disease of soft-shelled turtles and preparation method thereof - Google Patents
Traditional Chinese medicine prescription for treating red-neck disease of soft-shelled turtles and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine prescription for treating the red-neck disease of soft-shelled turtles. The traditional Chinese medicine prescription consists of the following ingredients: glycyrrhiza uralensis, gall nuts, radix isatidis, honeysuckle, flos chrysanthemi indici, rhubarb, cortex fraxini, cordate houttuynia, crataegus pinnatifida, coptis root, skullcap root, cortex phellodendri, rhizome alismatis, angelica sinensis, cnidium monnieri, radix asparagi, forsythia, myrobalan fruit, artemisia annua, black plum, garlic, pangolin scales, paulownia flower, cayratia japonica, solanum lyratum thunb and blackberry lily. The traditional Chinese medicine prescription disclosed by the invention can be used for treating the red-neck disease, is remarkable in treatment effect and free from side effect, and meanwhile is antibacterial and antiviral; although strongly-flavored materials, such as the garlic and the like, are used, the aims of removing flavors and not lowering treatment effects are achieved through reasonably combining the strongly-flavored materials and other drugs; the glycyrrhiza uralensis and the like are used for protecting liver cells injured by tannins of the gall nuts and the like; particularly, through using the Chinese herbal medicine together with western-medicine antibiotics and meanwhile adding ingredients, such as sodium bicarbonate and the like, the treatment effect can be further improved.
Description
Technical field
The present invention relates to a kind of veterinary drug, particularly relate to and be a kind ofly used for the treatment of prescription of Trionyx sinensis Wiegmann Red neck disease and preparation method thereof.
Background technology
The research of the nineties is pointed out, causing a disease of Trionyx sinensis Wiegmann Red neck disease was Aeromonas hydrophila originally, as described in " research of Trionyx sinensis Wiegmann Red neck disease ".Aeromonas hydrophila may cause various diseases, as skin fester disease, scabies disease.So its therapeutic scheme can with reference to this kind of antibacterial prescription.But recent research shows, Red neck disease is relevant with Trionyx sinensis Wiegmann iris virus, as described in " Prevention Technique of common diseases of soft-shelled turtle ".The prevention and control scheme of trionyx disease virus and the prevention and control scheme of antibacterial different.201210004505.6 relate to compound feed for young soft-shelled turtles and preparation method thereof, containing part Chinese medicine in its bait, it is said and may be used for treating Red neck disease.
Summary of the invention
The present invention proposes a kind of prescription being used for the treatment of Trionyx sinensis Wiegmann Red neck disease, and it may be used for treatment Trionyx sinensis Wiegmann Red neck disease, and Be very effective, can improve Trionyx sinensis Wiegmann antibacterium anti-virus ability simultaneously, the ability of especially anti-Aeromonas, the sick virus of iris.
For solving the problems of the technologies described above, the present invention constructs following technical proposals:
A kind of prescription being used for the treatment of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Lonicerae 20 parts, Flos Chrysanthemi Indici 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, Fructus Crataegi 18 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, 12 parts, Bulbus Allii, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
A kind of prescription being used for the treatment of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Chrysanthemi Indici 30 parts, Flos Lonicerae 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, 12 parts, Bulbus Allii, Fructus Crataegi 12 parts, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
A kind of prescription being used for the treatment of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Lonicerae 30 parts, Flos Chrysanthemi Indici 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, 20 parts, Bulbus Allii, Fructus Crataegi 18 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
Be used for the treatment of a preparation method for the prescription of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, comprise the following steps:
Pulverized by Fructus Crataegi, add the water of 2 times of quality, decoct 2h, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor;
By Radix Et Rhizoma Rhei, Flos Chrysanthemi Indici, Flos Lonicerae, Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid;
Get the medicinal herb components of residue kind, pulverize, add the water of twice quality, be warming up to 50 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 20min, filter, obtain filtrate;
Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, namely make described prescription.
A kind of spray agent being used for the treatment of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: Chinese herbal medicine 100 parts, sodium bicarbonate 8 ~ 10 parts, acetic acid 8 ~ 10 parts, phosphoric acid 8 ~ 10 parts, norfloxacin 1 ~ 5 part, vitamin C 4 parts, oxytetracycline 1 ~ 5 part.
Be used for the treatment of a preparation method for the spray agent of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, comprise the following steps:
Prepare prescription, after adding sodium bicarbonate, norfloxacin, be warming up to 60 DEG C, keep 2min, near room temperature, add acetic acid, phosphoric acid, vitamin C and oxytetracycline, after mix homogeneously, namely make described spray agent.
Prescription of the present invention may be used for treating Red neck disease, evident in efficacy, has no side effect, simultaneously anti-bacteria and anti-virus.Although employ the highly seasoned materials such as Bulbus Allii, reasonable compatibility other drug, reaches the object de-tasting and do not go curative effect.Radix Glycyrrhizaes etc. protect the hepatocyte injured by the tannin of Galla Chinensis etc.Especially itself and western medicine antibiotics are share, add the compositions such as sodium bicarbonate simultaneously, curative effect can be improved further.
Detailed description of the invention
Describe in detail the present invention below, unaccomplished matter is see the routine techniques knowledge of pharmacopeia 2010 or aquaculture field.
Embodiment one
Be used for the treatment of the prescription of Trionyx sinensis Wiegmann Red neck disease, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Lonicerae 20 parts, Flos Chrysanthemi Indici 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, Fructus Crataegi 18 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, 12 parts, Bulbus Allii, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
Preparation method is as follows: pulverized by Fructus Crataegi, add the water of 2 times of quality, decoct 2h, extracting juice, and Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor; By Radix Et Rhizoma Rhei, Flos Chrysanthemi Indici, Flos Lonicerae, Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid; Get the medicinal herb components of residue kind, pulverize, add the water of twice quality, be warming up to 50 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 20min, filter, obtain filtrate; Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, namely make described prescription.
Yield rate 1g/ml refers to the fried 1ml prescription (water preparation) of 1g raw material.Slow fire adopts medical science routine to understand, and refers to and refers to that fire when decocting medicinal herbs is little and slow, generally must not in 1min boiled 1L water.
Embodiment two
Be used for the treatment of the prescription of Trionyx sinensis Wiegmann Red neck disease, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Chrysanthemi Indici 30 parts, Flos Lonicerae 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, 12 parts, Bulbus Allii, Fructus Crataegi 12 parts, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
Embodiment three
Be used for the treatment of the prescription of Trionyx sinensis Wiegmann Red neck disease, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Lonicerae 30 parts, Flos Chrysanthemi Indici 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, 20 parts, Bulbus Allii, Fructus Crataegi 18 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
Embodiment four
Be used for the treatment of the spray agent of Trionyx sinensis Wiegmann Red neck disease, according to the mass fraction, consist of the following composition: prescription shown in embodiment three 100 parts, sodium bicarbonate 9 parts, acetic acid 9 parts, phosphatase 79 part, norfloxacin 3 parts, vitamin C 4 parts, oxytetracycline 3 parts.
Preparation method: prepare prescription, after adding sodium bicarbonate, norfloxacin, is warming up to 60 DEG C, keeps 2min, near room temperature, adds acetic acid, phosphoric acid, vitamin C and oxytetracycline, namely make described spray agent after mix homogeneously.
Experiment one
Test method:
1, plant is divided into the test tank of equal-volume (50cm × 50cm × 40cm), is labeled as 30 groups according to Chinese medicine ingredients.Often group adopts same water source (tap water after lake water or aeration), without being communicated with between group.Water temperature 20 to 28 DEG C, pH=6-8.
2, get Trionyx sinensis Wiegmann 600 tail at random in ill plant, be placed in test tank by 20 tails/component.Every day throws in bait by the 3-4% of Trionyx sinensis Wiegmann body weight, and 8: 00,13: 00,16: 00 respectively throw something and feed once.Every day puts half water from bottom, injects water source to 60 ± 10cm simultaneously.
3, daily check, takes out dead or is about to dead Trionyx sinensis Wiegmann, in order to avoid cross infection, affecting result of the test.
Experimental Comparison:
Different Chinese herbal medicine decoctions is thrown according to group name.According to reality survival number, delimit the effect of the monomer in treating Red neck disease of each Chinese medicine ingredients as following table: it is better that "+" represents curative effect more.
Through test, the present invention chooses some drugs, simultaneously proportion optimizing.Bulbus Allii plays therapeutic effect, and Flos Chrysanthemi Indici, Flos Lonicerae, Fructus Crataegi etc. de-taste.Radix Glycyrrhizae can also hepatoprotective.
Test two
Test material and environment: test Trionyx sinensis Wiegmann derives from breeding soft-shell turtle field, Nanchang City, Jiangxi Province, and the gross area is about 980m
2, total Trionyx sinensis Wiegmann number about 30000, there is Red neck disease in this plant, Trionyx sinensis Wiegmann is sporadicly dead.Trionyx sinensis Wiegmann test site is located at Duchang County, Jiangxi Province, is less than 10km apart from poyang lake water, and environment is suitable for Trionyx sinensis Wiegmann existence.Between test period February to April, cultivation 30d.
Test method:
1, plant is divided into six groups of test tanks of equal-volume (300cm × 300cm × 80cm), be labeled as A experimental group, B experimental group, C experimental group, D test group, A matched group, B matched group (matched group is common reference group, mentions in other experiments simultaneously) respectively.Often group adopts same water source (tap water after lake water or aeration), without being communicated with between group.Water temperature 20 to 28 DEG C, pH=6-8.
2, get Trionyx sinensis Wiegmann 600 tail at random in ill plant, be placed in six groups of test tanks by 100 tails/component.Every day throws in bait by the 3-4% of Trionyx sinensis Wiegmann body weight, and 8: 00,13: 00,16: 00 respectively throw something and feed once.Every day puts half water from bottom, injects water source to 60 ± 10cm simultaneously.
3, daily check, takes out dead or is about to dead Trionyx sinensis Wiegmann, in order to avoid cross infection, affecting result of the test.
Experimental Comparison:
In A experimental group, B experimental group, C experimental group, spray the prescription of embodiment one to three while throwing in bait respectively, dose concentration 50ml/m
3.Experimental group D throws in the spray agent of embodiment four, dose concentration 50ml/m
3.Control group A throws in antibiotic (oxytetracycline) 15g/m
3.Matched group B does not throw in medicine.
Along with the passing of culturing time, in test tank, dead Trionyx sinensis Wiegmann mantissa is as following table:
Red neck disease causes a disease because of complexity, may produce under antibacterial and viral combined effect.The antiviral such as Galla Chinensis, Bulbus Allii etc. are antibacterial, Rhizoma Belamcandae, compatibility share, and cures Red neck disease.Be used alone antibiotic, early stage is good to bacteriostatic ability, but the later stage is weak.Medicine of the present invention can play certain inhibition early stage, and the later stage can play therapeutical effect, eliminates the threat of Red neck disease completely.If a small amount of Western medicine of compatibility simultaneously, curative effect is more remarkable.In addition, when the concentration of Bulbus Allii is inadequate, offer limited effectiveness, if thrown in a large number, can face again the neural danger of injury Trionyx sinensis Wiegmann, the later death quantity of experimental group is few, illustrates that Bulbus Allii side effect is little.Galla Chinensis, Fructus Chebulae etc. have injury to liver, and the liver protection effect of Radix Glycyrrhizae etc. is also this prescription benefit.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. one kind is used for the treatment of the prescription of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Lonicerae 20 parts, Flos Chrysanthemi Indici 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, Fructus Crataegi 18 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, 12 parts, Bulbus Allii, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
2. one kind is used for the treatment of the prescription of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Chrysanthemi Indici 30 parts, Flos Lonicerae 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, 12 parts, Bulbus Allii, Fructus Crataegi 12 parts, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
3. one kind is used for the treatment of the prescription of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: 30 parts, Radix Glycyrrhizae, Galla Chinensis 30 parts, Radix Isatidis 30 parts, Flos Lonicerae 30 parts, Flos Chrysanthemi Indici 20 parts, Radix Et Rhizoma Rhei 20 parts, Cortex Fraxini 20 parts, Herba Houttuyniae 20 parts, 20 parts, Bulbus Allii, Fructus Crataegi 18 parts, Rhizoma Coptidis 18 parts, Radix Scutellariae 18 parts, Cortex Phellodendri 18 parts, Rhizoma Alismatis 18 parts, Radix Angelicae Sinensis 12 parts, Fructus Cnidii 12 parts, Radix Asparagi 12 parts, Fructus Forsythiae 12 parts, Fructus Chebulae 12 parts, Herba Artemisiae Annuae 12 parts, Fructus Mume 12 parts, Squama Manis 9 parts, Flos Paulowniae 9 parts, Herba Cayatiae Japonicae 9 parts, Herba Solani Lyrati 9 parts, Rhizoma Belamcandae 9 parts.
4. be used for the treatment of a preparation method for the prescription of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, comprise the following steps:
Pulverized by Fructus Crataegi, add the water of 2 times of quality, decoct 2h, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor;
By Radix Et Rhizoma Rhei, Flos Chrysanthemi Indici, Flos Lonicerae, Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid;
Get the medicinal herb components of residue kind, pulverize, add the water of twice quality, be warming up to 50 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 20min, filter, obtain filtrate;
Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, namely make described prescription.
5. one kind is used for the treatment of the spray agent of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, according to the mass fraction, consist of the following composition: Chinese herbal medicine 100 parts, sodium bicarbonate 8 ~ 10 parts, acetic acid 8 ~ 10 parts, phosphoric acid 8 ~ 10 parts, norfloxacin 1 ~ 5 part, vitamin C 4 parts, oxytetracycline 1 ~ 5 part.
6. be used for the treatment of a preparation method for the spray agent of Trionyx sinensis Wiegmann Red neck disease, it is characterized in that, comprise the following steps:
Prepare prescription, after adding sodium bicarbonate, norfloxacin, be warming up to 60 DEG C, keep 2min, near room temperature, add acetic acid, phosphoric acid, vitamin C and oxytetracycline, after mix homogeneously, namely make described spray agent.
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