CN108853250A - A method of prevention and treatment soft-shelled turtle Red neck disease - Google Patents
A method of prevention and treatment soft-shelled turtle Red neck disease Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K31/16—Amides, e.g. hydroxamic acids
- A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
- A61K31/167—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide having the nitrogen of a carboxamide group directly attached to the aromatic ring, e.g. lidocaine, paracetamol
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/315—Isatis, e.g. Dyer's woad
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- A61K36/78—Saururaceae (Lizard's-tail family)
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The present invention provides a kind of methods for preventing and treating soft-shelled turtle Red neck disease, extracting solution is made using honeysuckle, ginkgo leaf, Radix Isatidis, cordate houttuynia, paracetamol in this method, illness soft-shelled turtle is put into extracting solution daily and puts 20-30min in a suitable place to breed, treatment 7 days, this method is for preventing and treating soft-shelled turtle Red neck disease significant effect, the use that can avoid and reduce antibiotic and chemicals, more meets the production requirement of green ecological aquatic products.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a method of prevention and treatment soft-shelled turtle Red neck disease.
Background technique
Soft-shelled turtle is also known as soft-shelled turtle or the soft-shelled turtle, is a kind of oviparity amphibians faunal, head portrait tortoise, but carapace does not have the item as tortoise
Line, edge are in soft shape skirt, and color is blackish green.Soft-shelled turtle often lives in water-bed silt, is fond of the toys such as fish, shrimp, melon skin
Fruit bits, green grass and cereal etc. are also eaten.Turtle delicious is up to 16.5 grams rich in the every hectogram meat protein of protein, rouge
Fat content is higher while rich in multiple elements such as calcium, phosphorus, iron, and nutritive value is very high, is nourishing treasure.
Soft-shelled turtle is the favorite rare aquatic products of China common people, with the raising of living standards of the people, the market prospects of soft-shelled turtle
It is expected to rise always.Breeding soft-shell turtle is supported from original Zhejiang, Guangdong, Guangxi, Hunan, Hubei, Jiangsu and other places as China soft-shelled turtle
It grows main producing region to promote toward north always, but its pest and disease damage becomes always the main reason for raiser loses by emphasis.Wherein, soft-shelled turtle
Red neck disease is the more serious one kind of soft-shelled turtle disease, caused by virus, Symptoms be soft-shelled turtle neck it is rubescent, it is congested,
Enlargement, the death rate are very high.Currently used control method is using the drugs such as intramuscular injection aureomycin, chloramphenicol, dosage 150,000
Units/kg weight, between the back leg base portion and web of soft-shelled turtle, about 1.5cm is deep for injection site.But the above method uses chemical drugs
Object or antibiotic are not able to satisfy the production requirement of green ecological aquatic products.
Summary of the invention
For problem of the existing technology, the present invention provides a kind of method for preventing and treating soft-shelled turtle Red neck disease, this method
Extracting solution is made using honeysuckle, ginkgo leaf, Radix Isatidis, cordate houttuynia, paracetamol, illness soft-shelled turtle is put into extracting solution
Put 20-30min in a suitable place to breed, this method can avoid and reduce antibiotic and chemicals for preventing and treating soft-shelled turtle Red neck disease significant effect
Use, more meet the production requirement of green ecological aquatic products.
Specifically, the present invention provides a kind of method for preventing and treating soft-shelled turtle Red neck disease, include the following steps:
(1) by weight, extracting honeysuckle 200-500 parts, 200-500 parts of ginkgo leaf, 100-300 parts of Radix Isatidis, fish raw meat
It is 100-300 parts careless, add the 8-12 times of water boiling and extraction 30-60min measured, filters, obtain extracting solution;
(2) by weight, 2-6 parts of paracetamol are taken, extracting solution made from step (1) is put into, is sufficiently stirred molten
Solution obtains prevention and treatment soft-shelled turtle Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 20-30min, even
Continuous treatment 7 days, can fully recover;
Step (1) described above by weight, 300-400 parts of extracting honeysuckle, 300-400 parts of ginkgo leaf, Radix Isatidis
150-250 parts, 150-250 parts of cordate houttuynia, the water boiling and extraction 45min of 10 times of amounts, filtering obtain extracting solution;
Further, the step (1) by weight, 350 parts of extracting honeysuckle, 350 parts of ginkgo leaf, Radix Isatidis 200
Part, 200 parts of cordate houttuynia, the water boiling and extraction 45min of 10 times of amounts, filtering obtain extracting solution;
(2) described above by weight, take 3-5 parts of paracetamol, are put into extracting solution made from step (1),
Dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle Red neck disease medical fluid;
Further, (2) by weight, take 4 parts of paracetamol, are put into step (1) extraction obtained
Dissolution is sufficiently stirred in liquid, obtains prevention and treatment soft-shelled turtle Red neck disease medical fluid;
Step (3) described above will suffer from the isolation of Red neck disease soft-shelled turtle, be put into medical fluid made from step (2) daily and impregnate trip
Dynamic 25min, continuous treatment 7 days, can fully recover;
Beneficial effects of the present invention are:
The pathogenesis of present invention follow-up soft-shelled turtle Red neck disease is studied, on the basis of many years cultivating rich experiences,
It is screened by Optimum Experiment, it is final to determine with honeysuckle, ginkgo leaf, Radix Isatidis, cordate houttuynia Chinese medicine extract compatibility to acetyl ammonia
Base phenol is used to prevent and treat soft-shelled turtle Red neck disease effect highly significant, it can avoid and reduce the use of antibiotic and chemicals,
More meet the production requirement of green ecological aquatic products.
Specific embodiment
To be further described further to beneficial effects of the present invention, the present invention is in conjunction with the embodiments and part test example is made
It is further described, it should be appreciated that cited embodiment and test example of the invention is intended to illustrate the beneficial effect of invention
Fruit, but protection scope of the present invention is not restricted to this.
The method of the prevention and treatment soft-shelled turtle Red neck disease of embodiment 1
(1) extracting honeysuckle 200g, ginkgo leaf 200g, Radix Isatidis 100g, cordate houttuynia 100g add the water boiling and extraction of 8 times of amounts
30min, filtering, obtains extracting solution;
(2) paracetamol 2g is taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle
Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 20min, continuously control
It treats 7 days, it is efficient up to 92.7%.
The method of the prevention and treatment soft-shelled turtle Red neck disease of embodiment 2
(1) extracting honeysuckle 300g, ginkgo leaf 300g, Radix Isatidis 150g, cordate houttuynia 150g add the water boiling and extraction of 9 times of amounts
40min, filtering, obtains extracting solution;
(2) paracetamol 3g is taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle
Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 20min, continuously control
It treats 7 days, it is efficient up to 93.1%.
The method of the prevention and treatment soft-shelled turtle Red neck disease of embodiment 3
(1) extracting honeysuckle 350g, ginkgo leaf 350g, Radix Isatidis 200g, cordate houttuynia 200g add the water boiling and extraction of 10 times of amounts
45min, filtering, obtains extracting solution;
(2) paracetamol 4g is taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle
Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 25min, continuously control
It treats 7 days, it is efficient up to 93.8%.
The method of the prevention and treatment soft-shelled turtle Red neck disease of embodiment 3
(1) extracting honeysuckle 350g, ginkgo leaf 350g, Radix Isatidis 200g, cordate houttuynia 200g add the water boiling and extraction of 10 times of amounts
45min, filtering, obtains extracting solution;
(2) paracetamol 4g is taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle
Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 25min, continuously control
It treats 7 days, it is efficient up to 93.8%.
The method of the prevention and treatment soft-shelled turtle Red neck disease of embodiment 4
(1) extracting honeysuckle 400g, ginkgo leaf 400g, Radix Isatidis 250g, cordate houttuynia 250g add the water boiling and extraction of 12 times of amounts
50min, filtering, obtains extracting solution;
(2) paracetamol 5g is taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle
Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 25min, continuously control
It treats 7 days, it is efficient up to 93.4%.
The method of the prevention and treatment soft-shelled turtle Red neck disease of embodiment 5
(1) extracting honeysuckle 500g, ginkgo leaf 500g, Radix Isatidis 300g, cordate houttuynia 300g add the water boiling and extraction of 12 times of amounts
60min, filtering, obtains extracting solution;
(2) paracetamol 6g is taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains prevention and treatment soft-shelled turtle
Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 30min, continuously control
It treats 7 days, it is efficient up to 93.6%.
Test example investigates different control methods to the therapeutic effect of soft-shelled turtle Red neck disease
Present invention isolation suffers from soft-shelled turtle 160 of Red neck disease, is randomly divided into 4 groups, every group 40, test group 1 is using this
The method of inventive embodiments 3 is prevented and treated, and test group 2 is mould using intramuscular injection gold using intramuscular injection chloramphenicol, test group 3
Element, 4 feeding paracetamol of test group investigate treatment condition, such as the following table 1:
Therapeutic effect of the different control methods of table 1 to soft-shelled turtle Red neck disease
Above-mentioned experiment shows that control method and antibiotic group of the invention are substantially suitable, mainly uses Chinese medicine compatibility
Paracetamol is treated, and be can avoid and is reduced the use of antibiotic and chemicals, more meet green ecological aquatic products
Production requirement.
Claims (6)
1. a kind of method for preventing and treating soft-shelled turtle Red neck disease, which is characterized in that include the following steps:
(1) by weight, extracting honeysuckle 200-500 parts, 200-500 parts of ginkgo leaf, 100-300 parts of Radix Isatidis, cordate houttuynia
100-300 parts, add the 8-12 times of water boiling and extraction 30-60min measured, filters, obtain extracting solution;
(2) by weight, 2-6 parts of paracetamol are taken, extracting solution made from step (1) is put into, dissolution is sufficiently stirred, obtains
To prevention and treatment soft-shelled turtle Red neck disease medical fluid;
(3) isolation of Red neck disease soft-shelled turtle will be suffered from, medical fluid made from step (2) is put into daily and impregnates travelling 20-30min, continuously control
It treats 7 days, can fully recover.
2. the method for prevention and treatment soft-shelled turtle Red neck disease according to claim 1, it is characterised in that:Step (1) by weight,
300-400 parts of extracting honeysuckle, 300-400 parts of ginkgo leaf, 150-250 parts of Radix Isatidis, 150-250 parts of cordate houttuynia, the water of 10 times of amounts
It decocts and extracts 45min, filtering obtains extracting solution.
3. the method for prevention and treatment soft-shelled turtle Red neck disease according to claim 1 or 2, it is characterised in that:The step (1) is pressed
Parts by weight meter, 350 parts of extracting honeysuckle, 350 parts of ginkgo leaf, 200 parts of Radix Isatidis, 200 parts of cordate houttuynia, the water boiling and extraction of 10 times of amounts
45min, filtering, obtains extracting solution.
4. the method for prevention and treatment soft-shelled turtle Red neck disease according to claim 1, it is characterised in that:(2) are by weight
Meter, takes 3-5 parts of paracetamol, is put into extracting solution made from step (1), dissolution is sufficiently stirred, and obtains prevention and treatment soft-shelled turtle ruby throat
Sub- disease medical fluid.
5. the method for prevention and treatment soft-shelled turtle Red neck disease according to claim 1 or 2, it is characterised in that:(2) are by weight
Part meter, takes 4 parts of paracetamol, is put into extracting solution made from step (1), dissolution is sufficiently stirred, and obtains prevention and treatment soft-shelled turtle ruby throat
Sub- disease medical fluid.
6. the method for prevention and treatment soft-shelled turtle Red neck disease according to claim 1, it is characterised in that:The step (3) will be suffered from
The isolation of Red neck disease soft-shelled turtle is put into medical fluid made from step (2) daily and impregnates travelling 25min, and continuous treatment 7 days can fully recover.
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CN104435632A (en) * | 2014-11-04 | 2015-03-25 | 占水秀 | Traditional Chinese medicine prescription for treating red-neck disease of soft-shelled turtles and preparation method thereof |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104435632A (en) * | 2014-11-04 | 2015-03-25 | 占水秀 | Traditional Chinese medicine prescription for treating red-neck disease of soft-shelled turtles and preparation method thereof |
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Title |
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