CN106072569B - The special dietary seafood of patient's immunocyte balance is adjusted during a kind of chemicotherapy - Google Patents
The special dietary seafood of patient's immunocyte balance is adjusted during a kind of chemicotherapy Download PDFInfo
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Abstract
The present invention relates to a kind of special dietary seafoods for tumor chemoradiotherapy phase edible for patients.In order to solve the damage of radiotherapy and chemotherapy medicine normal cell caused by the blocking of normal cell production cycle and the inhibition of gene, spy's dietary seafood includes the raw material of following parts by weight: 30-350 parts of barley seedling, 30-165 parts of green tea, 25-145 parts of mulberry leaf, 30-145 parts of lotus leaf, 35-275 parts of honeysuckle, 10-35 parts of radix polygonati officinalis, 40-55 parts of chrysanthemum, 32-45 parts of fructus lycii, 32-45 parts of cordate houttuynia, 30-55 parts of dandelion, 20-55 parts of lemon, 42-55 parts of semen coicis, 20-55 parts of lily, 20-55 parts of orange peel, 30-55 parts of rhizoma polygonati, 20-55 parts of cape jasmine, 20-55 parts of carrot, 20-75 parts of curd, 20-50 parts of germanium-enriched barley seedling, 20-50 parts of selenium-rich barley seedling.Spy's dietary seafood, it is with strong points, dedicated for adjusting the exception that radiotherapy and chemotherapy medicine causes immunocyte, immunocyte is made to keep balance.
Description
Technical field
The present invention relates to a kind of special dietary seafood, specifically a kind of special dietary seafoods for tumor chemoradiotherapy phase edible for patients.
Background technique
Tumour is body under the effect of various carcinogenic factors, some cell of local organization loses pair at the genetic level
Its normal regulation grown leads to its clonal abnormality hyperplasia and the abnormality that is formed.It is thin from first cancer is generated in human body
Born of the same parents develop to the apparent cancer of clinical symptoms, require the time of 20--30 greatly.
The main reason for tumour generates is: (1) chemical carcinogenic factor: such as polycyclic aromatic hydrocarbons, amino azo dyes, nitrous
Amine, mycotoxin, vinyl chloride and certain metals, such as chromium, nickel, arsenic can be carcinogenic.(2) physics carcinogenic factor: ionizing radiation
Cause various cancers.(3) virus and bacterium are carcinogenic: RNA, DNA oncogenic virus are whole by inhereditary material by transduction and insertion mutation
With into host cell DNA and make host cell occur conversion it is carcinogenic.Tumour patient once finds tumour and takes modern doctor mostly
Treat tumour, mainly performs the operation, and radiotherapy, chemotherapy etc., also some take conservative therapy, such as uses Traditional Chinese Medicine Chinese medicine
It is treated.The physiological status of each stage patient occurs significantly changing compared with normal physiological during this treatment
Become, in different treatments and Restoration stage in addition to corresponding treatment, patient is concerned about diet care very much, it may be said that each aobvious mind
Logical, anti-anti-cancer dietotherapy prescription emerges one after another, but is the different lifes occurred for the different phase of tumour without a kind of prescription
The imbalance of reason state carries out the dietotherapy for raise, thus having with a definite target in view and obtains preferable effect, some things
It is disobeyed with hope.The different treatment means and period taken according to patient are segmented, and can substantially be divided: (1) the tumour conservative therapy phase；
(2) tumour peri-operation period；(3) the tumor chemoradiotherapy phase；(4) tumor post-operation convalescence；(5) advanced tumor.
It is treatment means that chemicotherapy, which is using radioactive ray or anticancer drug, is killed to kinds cancer cell, have compared with
Good short term effect.But chemicotherapy, while killing cancer cell, also normal tissue cell non-selectivity kills, commonly referred to as
Toxic and side effects of chemoradiotherapy or adverse reaction.Clinically common: bone marrow suppression, side effect of digestive tract, organ functional lesion are immunized
Function reduces, alopecia, skin injury, phlebitis, canker sore etc..
Summary of the invention
It is an object of the invention to solve radiotherapy and chemotherapy medicine to make the blocking of normal cell production cycle and the inhibition of gene
At normal cell damage, the special dietary seafood of patient's immunocyte balance is adjusted during a kind of chemicotherapy is provided.
The present invention is directed to chemicotherapy medicine normal cell caused by the blocking of normal cell production cycle and the inhibition of gene
Following measure is taken in damage, the invention: (1) rich in antioxidant, there is stronger removal free radical, accelerate in-vivo tumour thin
The metabolism of born of the same parents' death toxin avoids strong immune of non-destructive tests mode (DAMP) caused by these death of neoplastic cells toxin
T lymphocyte is reduced because division growth of overrunning ultimately causes T lymphocyte by chemotherapeutic inhibition caused by response.(2) improve
The aseptic inflammation reaction of tumor microenvironment plays the role of adjusting immunocyte balance.
Special dietary seafood of the invention is achieved through the following technical solutions:
The special dietary seafood of patient's immunocyte balance is adjusted during a kind of chemicotherapy, the raw material including following parts by weight:
30-350 parts of barley seedling, 30-165 parts of green tea, 25-145 parts of mulberry leaf, 30-145 parts of lotus leaf, 35-275 parts of honeysuckle,
10-35 parts of radix polygonati officinalis, 40-55 parts of chrysanthemum, 32-45 parts of fructus lycii, 32-45 parts of cordate houttuynia, 30-55 parts of dandelion, 20-55 parts of lemon,
42-55 parts of semen coicis, 20-55 parts of lily, 20-55 parts of orange peel, 30-55 parts of rhizoma polygonati, 20-55 parts of cape jasmine, 20-55 parts of carrot,
20-75 parts of curd, 20-50 parts of germanium-enriched barley seedling, 20-50 parts of selenium-rich barley seedling；
The special dietary seafood preferred raw material weight range of patient's immunocyte balance is adjusted during chemicotherapy of the present invention
It is:
90-270 parts of barley seedling, 90-150 parts of green tea, 50-130 parts of mulberry leaf, 70-120 parts of lotus leaf, 80-240 parts of honeysuckle,
15-25 parts of radix polygonati officinalis, 42-50 parts of chrysanthemum, 36-42 parts of fructus lycii, 36-42 parts of cordate houttuynia, 36-50 parts of dandelion, 26-50 parts of lemon,
46-52 parts of semen coicis, 30-50 parts of lily, 26-50 parts of orange peel, 36-48 parts of rhizoma polygonati, 26-48 parts of cape jasmine, 30-48 parts of carrot,
40-68 parts of curd, 30-40 parts of germanium-enriched barley seedling, 30-40 parts of selenium-rich barley seedling；
The special dietary seafood optimum feed stock weight that patient's immunocyte balance is adjusted during chemicotherapy of the present invention is:
180 parts of barley seedling, 130 parts of green tea, 75 parts of mulberry leaf, 95 parts of lotus leaf, 125 parts of honeysuckle, 23 parts of radix polygonati officinalis, chrysanthemum 48
Part, 39 parts of fructus lycii, 38 parts of cordate houttuynia, 42 parts of dandelion, 43 parts of lemon, 48 parts of semen coicis, 40 parts of lily, 38 parts of orange peel, rhizoma polygonati
42 parts, 38 parts of cape jasmine, 38 parts of carrot, 50 parts of curd, 36 parts of germanium-enriched barley seedling, 36 parts of selenium-rich barley seedling；
The special dietary seafood that patient's immunocyte balance is adjusted during chemicotherapy of the present invention, can be made into Ultramicro-powder, tablet and glue
Capsule；
The special dietary seafood Ultramicro-powder that patient's immunocyte balance is adjusted during chemicotherapy of the present invention refers to that granularity is 200-500
Purpose particle；
The special dietary seafood that patient's immunocyte balance is adjusted during chemicotherapy of the present invention, can start to eat for 3 days before chemicotherapy
With can also be taken with Miles' operation, take 5-50g daily, until the chemicotherapy course for the treatment of terminates.
The special dietary seafood action principle of patient's immunocyte balance is adjusted during chemotherapy of the present invention:
It is green that the present invention adjusts the leaf being rich in the special dietary seafood that patient's immunocyte balances during mainly passing through chemicotherapy
Element, flavones, Barley Greeg polyphenol, blue and white element, beta carotene, selenium-rich, Fu Zhe, superoxide dismutase, alkaloid etc. have relatively strong
Removal free radical, accelerate interior tumor cell death toxin metabolism, avoid these death of neoplastic cells toxin and draw
T lymphocyte is pressed down because overrunning division growth by chemotherapeutic caused by the strong immune response of the non-destructive tests mode (DAMP) risen
System ultimately causes T lymphocyte reduction, and the aseptic inflammation reaction for improving tumor microenvironment plays adjusting immunocyte and puts down
The effect of weighing apparatus.
While chemotherapeutics is to tumor cytotoxicity, immune system can also be destroyed, cause the disorderly of immune function
Disorderly, in the past in classic chemotherapy, for the reduction for preventing leucocyte, often cooperate some immunopotentiators, but sometimes up to less than anticipation
As a result, instead occur opposite as a result, may be with regard to its reason, when immunocyte is under the action of immunopotentiator, quickly
Division growth, chemotherapeutics are precisely to prevent fissional, and are regardless of fine or not cell, fissional faster lethality
It is bigger, thus a large amount of leucocyte is killed, and immune function dramatic decrease is caused, it loses and tumour cell is monitored and is inhibited
Effect, causes chemotherapy to fail, and having the plant of dual conciliation to immunocyte invention applies integration of drinking and medicinal herbs is raw material, such as:
Mulberry leaf: it is rich in rare element organic selenium, germanium, is natural powerful antioxidant, interior free yl can be removed, make to accumulate
It is oxidized in the intracorporal toxin of people and waste, increases the oxygen content in blood, boost metabolism and microcirculation；For cancer art
The rehabilitation of chemicotherapy patient has extraordinary booster action afterwards；Dispelling wind and heat from the body, clearing away the lungheat and moisturizing, suppressing liver-YANG clear liver and improve vision, cool blood
Hemostasis；
Lotus leaf: lotus leaf contains a variety of alkaloids such as nuciferine, miltanthin and Nuciferine and vitamin C, polysaccharide；There is heat-clearing solution
Poison, cool blood, hemostasis effect；
Fructus lycii: polysaccharides can significantly improve the phagocytic function of phagocyte, improve the proliferative capacity of lymphocyte；Chinese holly
Qi polysaccharide is not only a kind of biological response modifiers for adjusting immune response, and can pass through nerve -- endocrine -- immunological regulation
Network plays antitumaous effect；
Cordate houttuynia: cordate houttuynia can be obviously promoted human peripheral leucocytes phagocytic function in vitro, improve rabbit and patient's blood
Clear properdin is horizontal；
Radix polygonati officinalis: the steroid glucoside that radix polygonati officinalis contains has cardiac effect to the effect of cardiac muscle, and medicinal extract intraperitoneal injection can enhance burn
Turnover of Mouse Peritoneal Macrophages phagocytosis improves Hemolysin level, it is anti-to the proliferation of ConA to improve splenic lymphocytes
It answers；
In conclusion mulberry leaf, lotus leaf is rich in rare element organic selenium, germanium, and natural powerful antioxidant contains biology abundant
Alkali has clearing heat and detoxicating, the effect of cool blood, plays the role of reducing anti-inflammatory factors.And fructus lycii, cordate houttuynia, then have raising immune
The phagocytosis of cell improves the effect of anti-inflammatory factors.Work as mulberry leaf, lotus leaf then reaches balance with fructus lycii, the mutual compatibility of cordate houttuynia and exempts from
The effect of epidemic disease function, and the effect of the heart tonifying promoting blood circulation of radix polygonati officinalis is mixed, it is more advantageous to the equilibrium of immune cell, immune molecule,
Reach immunologic balance.
Chemotherapeutics has certain destruction to the organ of body, is especially to metabolic organ's liver kidney etc., the present invention
Applying integration of drinking and medicinal herbs has clearing heat and detoxicating raw material such as:
Honeysuckle: have anti-inflammatory and refrigeration function: intraperitoneal injection Flos Lonicerae extractive solution 0.25g/kg can inhibit rat angle to pitch
Dish colloidality foot is swollen, play the role of it is obvious it is impervious go out and anti-proliferate；
Chrysanthemum: isolated triterpene enediol, triol and its corresponding palmitate and myristinate pair in chrysanthemum
There is apparent anti-inflammatory effect by the mouse dropsy of ear that 12-O- myristoyl base-phorbol -13- ethyl alcohol ester induces；In addition from
Isolated taraxerane type triterpene alcohols has more significant suppression to the mouse skin tumour as caused by TPA in chrysanthemum
Production is used；
The toxic side effect of chemotherapeutics is mainly the lethal effect to cell, excites phagocytosis of the immunocyte to non-viable non-apoptotic cell
Cause aseptic inflammation, the anti-inflammatory synergistic effect of honeysuckle and chrysanthemum can reduce the toxic side effect of chemotherapeutic just.
When patient's treatment with chemotherapy drug, the discomfort of gastrointestinal tract can usually be such that chemotherapy interrupts, and the preset course for the treatment of, this hair is not achieved
The bright integration of drinking and medicinal herbs that applies has the raw material of improvement gastrointestinal symptoms such as:
Semen coicis: the antitumor action of the coix seed oil extracted in semen coicis is particularly evident, increases appetite and weight, also
Pain caused by cancer can be alleviated by providing body energetic nourishing；
Lily: there are the characteristics such as antitumor, anti-oxidant, enhancing immunity of organisms, hypoglycemic, antifatigue；
Barley seedling: rich in effective components such as chlorophyll, protein, amino acid, vitamin, minerals, organized enzymes, by the world
Various regions scientific circles are referred to as " king of base-forming food "；A large amount of enzymes therein have DNA damage reparation, anti-inflammatory analgesic, degradation carcinogenic
The functions such as burning, in addition also have and alleviate physical fatigue, improve liver function and other effects, anti-oxidant, liver protection, strengthen immunity
Etc. bioactivity；
The compatibility of semen coicis, lily and barley seedling is obvious, can improve vomiting caused by chemotherapy, increase appetite and weight,
Anti-inflammatory analgesic alleviates physical fatigue.
The invention has the advantages that
1, the special dietary seafood of patient's immunocyte balance is adjusted during chemicotherapy of the invention, it is with strong points, dedicated for
The exception that radiotherapy and chemotherapy medicine causes immunocyte is adjusted, immunocyte is made to keep balance.
The special dietary seafood of patient's immunocyte balance is adjusted during chemicotherapy of the invention, feature is with anti-oxidant, heat-clearing
Removing toxic substances, the natural food materials for reducing anti-inflammatory factors effect and medicinal and edible plant compatibility and arrive, with exempting from for conventional chemotherapy adapted
It is different that epidemic disease enhances nutriment.
The special dietary seafood of patient's immunocyte balance, adjustment effect mechanism and routine are adjusted during chemicotherapy of the invention
Chemotherapy adapted Immune enhanced nutrition product it is different, be not it is direct promote immunocyte, be chlorophyll, the Huang by being wherein rich in
Ketone, Barley Greeg polyphenol, blue and white element, beta carotene, selenium-rich, Fu Zhe, superoxide dismutase, alkaloid etc., there is stronger go
Except free radical, accelerate the metabolism of interior tumor cell death toxin, caused by avoiding these death of neoplastic cells toxin
T lymphocyte is inhibited most because overrunning division growth by chemotherapeutic caused by the strong immune response of non-destructive tests mode (DAMP)
The aseptic inflammation reaction for causing T lymphocyte to reduce eventually, and improving tumor microenvironment, which plays, adjusts immunocyte balance
Effect.
Detailed description of the invention
Fig. 1: S-180 oncocyte MTT testing result amplification factor 4 × 10 × 10；
Fig. 2: macrophage MTT testing result amplification factor 4 × 10 × 10；
Fig. 3: the fuselage state of each group mouse at the end of experiment；
The internal anatomy of Fig. 4: 3 groups of tumours；
In Fig. 1: the normal S-180 oncocyte b. of a. adds the S-180 oncocyte of the green powder of wheat (0.15mg/ml)
C. plus the S-180 oncocyte d. of 5-FU adds the S-180 oncocyte of levamisol
In Fig. 2: the normal macrophage b. of a. adds the macrophage of the green powder of wheat (0.15g/ml)
C. plus the macrophage d. of 5-FU adds the macrophage of levamisol
In Fig. 3: normal group of a.: the hair color of mouse is bright and smooth；B. tumour control group；The hair color of mouse is bright and smooth；
C. chemotherapy group: mouse chaeta, which is holded up, to be thinned out rough, and mouse is dispirited；D. the green powder group of wheat: the hair color of mouse it is bright and
It is smooth, active oxter tumor mass
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, those of ordinary skill in the art's every other embodiment obtained under the premise of no creative work, belongs to
The scope of protection of the invention.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 1, a kind of chemicotherapy
Raw material:
30 parts of barley seedling, 30 parts of green tea, 25 parts of mulberry leaf, 30 parts of lotus leaf, 35 parts of honeysuckle, 10 parts of radix polygonati officinalis, 40 parts of chrysanthemum, Chinese holly
32 parts of Qi, 32 parts of cordate houttuynia, 30 parts of dandelion, 20 parts of lemon, 42 parts of semen coicis, 20 parts of lily, 20 parts of orange peel, 30 parts of rhizoma polygonati,
20 parts of cape jasmine, 20 parts of carrot, 20 parts of curd, 20 parts of germanium-enriched barley seedling, 20 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 2, a kind of chemicotherapy
Raw material:
350 parts of barley seedling, 165 parts of green tea, 145 parts of mulberry leaf, 145 parts of lotus leaf, 275 parts of honeysuckle, 35 parts of radix polygonati officinalis, chrysanthemum 55
Part, 45 parts of fructus lycii, 45 parts of cordate houttuynia, 55 parts of dandelion, 55 parts of lemon, 55 parts of semen coicis, 55 parts of lily, 55 parts of orange peel, rhizoma polygonati
55 parts, 55 parts of cape jasmine, 55 parts of carrot, 75 parts of curd, 50 parts of germanium-enriched barley seedling, 50 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 3, a kind of chemicotherapy
Raw material:
190 parts of barley seedling, 98 parts of green tea, 85 parts of mulberry leaf, 88 parts of lotus leaf, 155 parts of honeysuckle, 22 parts of radix polygonati officinalis, 48 parts of chrysanthemum,
38 parts of fructus lycii, 38 parts of cordate houttuynia, 43 parts of dandelion, 38 parts of lemon, 48 parts of semen coicis, 37 parts of lily, 37 parts of orange peel, rhizoma polygonati 42
Part, 37 parts of cape jasmine, 37 parts of carrot, 47 parts of curd, 35 parts of germanium-enriched barley seedling, 35 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 4, a kind of chemicotherapy
Raw material:
90 parts of barley seedling, 90 parts of green tea, 50 parts of mulberry leaf, 70 parts of lotus leaf, 80 parts of honeysuckle, 15 parts of radix polygonati officinalis, 42 parts of chrysanthemum, Chinese holly
36 parts of Qi, 36 parts of cordate houttuynia, 36 parts of dandelion, 26 parts of lemon, 46 parts of semen coicis, 30 parts of lily, 26 parts of orange peel, 36 parts of rhizoma polygonati,
26 parts of cape jasmine, 30 parts of carrot, 40 parts of curd, 30 parts of germanium-enriched barley seedling, 30 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 5, a kind of chemicotherapy
Raw material:
270 parts of barley seedling, 150 parts of green tea, 130 parts of mulberry leaf, 120 parts of lotus leaf, 240 parts of honeysuckle, 25 parts of radix polygonati officinalis, chrysanthemum 50
Part, 42 parts of fructus lycii, 42 parts of cordate houttuynia, 50 parts of dandelion, 50 parts of lemon, 52 parts of semen coicis, 50 parts of lily, 50 parts of orange peel, rhizoma polygonati
48 parts, 48 parts of cape jasmine, 48 parts of carrot, 68 parts of curd, 40 parts of germanium-enriched barley seedling, 40 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 6, a kind of chemicotherapy
Raw material:
180 parts of barley seedling, 120 parts of green tea, 90 parts of mulberry leaf, 95 parts of lotus leaf, 160 parts of honeysuckle, 20 parts of radix polygonati officinalis, chrysanthemum 46
Part, 39 parts of fructus lycii, 39 parts of cordate houttuynia, 43 parts of dandelion, 38 parts of lemon, 49 parts of semen coicis, 40 parts of lily, 38 parts of orange peel, rhizoma polygonati
42 parts, 37 parts of cape jasmine, 39 parts of carrot, 54 parts of curd, 35 parts of germanium-enriched barley seedling, 35 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 7, a kind of chemicotherapy
Raw material:
180 parts of barley seedling, 130 parts of green tea, 75 parts of mulberry leaf, 95 parts of lotus leaf, 125 parts of honeysuckle, 23 parts of radix polygonati officinalis, chrysanthemum 48
Part, 39 parts of fructus lycii, 38 parts of cordate houttuynia, 42 parts of dandelion, 43 parts of lemon, 48 parts of semen coicis, 40 parts of lily, 38 parts of orange peel, rhizoma polygonati
42 parts, 38 parts of cape jasmine, 38 parts of carrot, 50 parts of curd, 36 parts of germanium-enriched barley seedling, 36 parts of selenium-rich barley seedling.
The special dietary seafood of patient's immunocyte balance, including following parts by weight are adjusted during embodiment 8, a kind of chemicotherapy
Raw material:
35 parts of barley seedling, 68 parts of green tea, 25 parts of mulberry leaf, 55 parts of lotus leaf, 37 parts of honeysuckle, 10 parts of radix polygonati officinalis, 40 parts of chrysanthemum, Chinese holly
35 parts of Qi, 32 parts of cordate houttuynia, 30 parts of dandelion, 25 parts of lemon, 50 parts of semen coicis, 42 parts of lily, 28 parts of orange peel, 36 parts of rhizoma polygonati,
27 parts of cape jasmine, 25 parts of carrot, 70 parts of curd, 50 parts of germanium-enriched barley seedling, 50 parts of selenium-rich barley seedling.
Match raw material in proportion, remove impurity, clean, dried at 45-55 DEG C, pulverize, granularity reaches 200-500 mesh, micro-
60-80 DEG C of wave sterilization, 2-4 minutes, packaging, 6g/ bags.The above whole preparation process meets national food safety standard " special doctor
Learn purposes formula food Good Manufacture Practice " GB 29923-2013, it is pre-packaged special that packaging meets national food safety standard
Diet and foodstuffs label G B 13432-2013.
Application Example 1.The green powder of wheat (referring to the special dietary seafood in embodiment 8) is in vitro to S-180 oncocyte inhibiting effect
Take logarithmic phase grow S-180 oncocyte, with containing 10% FBS 1640 culture solution of RPMI be made into density be 5 ×
The cell suspension of 104~2 × 105/mL is inoculated in 96 well culture plates, every hole 100 μ L, 37 DEG C, 5%CO2 incubator training 2h
Afterwards, if experimental group: wheat 4 gradients of green powder；Chemotherapeutics control group: 5 FU 5 fluorouracil (5-FU)；Immunopotentiator control group: salt
Sour levamisol；Negative control group: RPMI 1640 culture medium, every group sets 6 repetitions, takes 100 μ L to be added the above each group drug
In the culture hole of inoculating cell.Routine culture, for 24 hours after, 20 μ L, 5 mg/mL MTT solution is added in every hole, continue to cultivate 4h,
Culture is terminated, supernatant in hole is discarded.150 μ L DMSO are added in every hole, and shaking table vibrates 10min, survey 490 nm of wavelength with microplate reader
Locate OD value, calculate S-180 cell inhibitory rate, see that formula is as follows:
Inhibitory rate of cell growth=(OD2-OD1)/OD2 × 100%, in formula: OD1 is that experimental group is averaged OD value；OD2 is
Control group mean OD value.
The green powder of the wheat of a concentration plays the role of inhibiting S-180 oncocyte, and concentration is in 0.05g/ml-0.15g/ml,
Inhibiting rate is positively correlated with dosage；When concentration is greater than 0.15 g/ml, the green powder of wheat is to the inhibiting rate of S-180 oncocyte in decline
Trend.Levamisole hydrochloride is negative value to the inhibiting rate of S-180 oncocyte.The green powder of the wheat of 4 concentration has compared with 5-Fu group
There is extremely significant difference (P ﹤ 0.01).Such as table 1 and Fig. 1
Influence of the green powder of wheat of 1. various concentration of table to S-180 oncocyte
Note: P < 0.01 experimental group * P < 0.05 compared with 5-FU group, * *
Application Example 2.The green powder of wheat (referring to the special dietary seafood in embodiment 8) in vitro acts on macrophage proliferation
A healthy mice is chosen, the neck that breaks is put to death, and is rinsed collection peritoneal macrophage with RPMI 1640 culture medium and is extremely centrifuged
Guan Zhong, 1500 r/min are centrifuged 5min, abandon supernatant, are resuspended with 1640 basal medium of RPMI, and cell density is adjusted to
It 5 × 104~2 × 105/ml, is inoculated into 96 porocyte culture plates, every hole 100 μ L, 37 DEG C, 5%CO2 incubator culture 2h,
Thereafter with embodiment 1, experiment terminates to calculate macrophage proliferation rate for experimental group and experimental method, sees that formula is as follows:
Growth and proliferation of cell rate=OD1/OD2 × 100%, in formula: OD1 is that experimental group is averaged OD value；OD2 is control group
Mean OD value.
The green powder of the wheat of a concentration is on a declining curve with concentration increase to macrophage proliferation rate.The green powder of the wheat of 4 concentration
Compared with levamisole hydrochloride group, difference that there are no significant.As shown in table 2 and figure 2.
Influence of the green powder of wheat of 2. various concentration of table to macrophage
Application Example 3.Effect of the green powder of wheat (referring to the special dietary seafood in embodiment 8) to tumor-bearing mice during chemotherapy
Mouse tumor model is initially set up, single cell suspension is made in the mouse S-180 cell for taking logarithmic phase to grow, and adjusts
Cell concentration is 1 × 107/mL, under aseptic condition with 1 mL syringe by S-180 cell inoculation in mouse right fore oxter
Subcutaneously, inoculum concentration is 0.2 mL/.Mouse meets the 7th d after tumor, and modeling mouse is randomly divided into 3 groups, every group 6.Chemotherapeutic
Group: intraperitoneal injection 5-FU, 0.2 ml/ only, 1 time/d, continuous 3d；The green powder group of wheat: stomach-filling wheat green powder while injection chemotherapeutic,
0.2 ml/, 1 time/d, continuous 15d.Tumour control group: stomach-filling physiological saline, dosage and time are the same as the green powder group of wheat.Normal control
Group: with no treatment.At the end of experiment, mouse weight, tumor matter weight are surveyed respectively, and liver and spleen quality breaks end and takes blood, five classification
Conventional determining haemocyte, and tumour inhibiting rate is calculated separately as follows, liver and spleen index.
Tumour inhibiting rate formula: inhibiting rate=(control group be averaged tumor quality-experimental group be averaged tumor quality)/control group is averaged tumor matter
Amount × 100%
Liver and spleen exponential formula: liver and spleen index=liver and spleen quality (mg)/weight (g) × 100%
(1) influence of the green powder of wheat to tumor-bearing mice body general state
Experiment front and back, the green powder group Mice Body hair color of wheat is bright and smooth, and mouse is active；Tumour control group: Mice Body hair color does not have
There is difference.Chemotherapy group: Mice Body hair color is bright before testing, and mouse chaeta, which is holded up, after experiment is thinned out rough, and mouse is dispirited.Such as
Fig. 3
(2) influence of the green powder of wheat to tumor-bearing mice weight and tumor quality
Weight: after experiment, normal group and tumour control group weight difference are positive value, illustrate weight after experiment
Be it is increased, the green powder group of wheat and chemotherapy group weight difference are negative values, illustrate experiment after weight be reduce.Tumor quality: wheat
Green powder group (p < 0.01) and chemotherapy group (p < 0.05) are substantially less than tumour control group, the green powder group of wheat be substantially less than chemotherapy group (p <
0.05).Tumour inhibiting rate: the tumour inhibitory rate of chemotherapy group to 45.9%, the inhibitory rate of the green powder group of wheat to 74.3%.It is shown in Table 3 and Fig. 4
Influence (x ± s, n=6) of the green powder of 3 wheat of table to tumor-bearing mice weight and tumor quality
Note: the green powder group of a. wheat is compared with tumour control group;B. the green powder group of wheat is compared with chemotherapy group, and * P < 0.05, * * P <
0.01
(3) influence of the green powder of wheat to tumor-bearing mice immune organ
Liver index: the green powder group of wheat, chemotherapy group and tumour control group are all remarkably higher than normal group (p < 0.05)；Spleen refers to
Number: the green powder group of wheat is significantly higher than tumour control group and chemotherapy group (p < 0.05).It is shown in Table 4
Influence (x ± s, n=6) of the green powder of 4 wheat of table to tumor-bearing mice immune organ
Note: the green powder group of a. wheat is compared with normal group;B. the green powder group of wheat is compared with tumour control group;C. the green powder group of wheat with
Chemotherapy group is compared, P < 0.01 * P < 0.05, * *
(4) influence of the green powder of wheat to tumor-bearing mice immunocyte
In 24 of blood routine examining report, immunocyte has been carried out statistical analysis and has been shown in Table 5 by we.
Erythrocytes: the green powder group of wheat and chemotherapy group are substantially less than normal group (p < 0.05).Total number of blood platelet: the green powder of wheat
Group, chemotherapy group are significantly higher than tumour control group (p < 0.05).Total white blood cells: experimental group and control group are without significant difference.Leaching
Bar cell absolute value: the green powder group of wheat is significantly higher than tumour control group (p < 0.05) and chemotherapy group (p < 0.01), and chemotherapy group is significantly low
In tumour control group (p < 0.05).Monocyte absolute value: the green powder group of wheat and chemotherapy group be significantly higher than tumour control group (p <
0.01), and the green powder group of wheat is significantly higher than chemotherapy group (p < 0.01).Neutrophil cell absolute value: the green powder group of wheat is significantly higher than swollen
Tumor control group (p < 0.01) and chemotherapy group (p < 0.05).Eosinophil absolute value: the green powder group of wheat be significantly higher than chemotherapy group (p <
And tumour control group (p < 0.01) 0.05).Basophilic granulocyte absolute value: the green powder group (p < 0.01) of wheat and chemotherapy group (p < 0.05)
Substantially less than tumour control group.
Influence (x ± s, n=6) of the green powder of 5 wheat of table to the thin immunocyte of tumor-bearing mice
Note: the green powder group of a. wheat is compared with normal group;B. the green powder group of wheat is compared with tumour control group;C. the green powder group of wheat with
Chemotherapy group compares, P < 0.01 * P < 0.05, * *.
Claims (7)
1. adjusting the special dietary seafood of patient's immunocyte balance during a kind of chemicotherapy, it is characterised in that: including following parts by weight
Several raw materials:
30-350 parts of barley seedling, 30-165 parts of green tea, 25-145 parts of mulberry leaf, 30-145 parts of lotus leaf, 35-275 parts of honeysuckle, radix polygonati officinalis
10-35 parts, 40-55 parts of chrysanthemum, 32-45 parts of fructus lycii, 32-45 parts of cordate houttuynia, 30-55 parts of dandelion, 20-55 parts of lemon, coix lacryma-jobi
42-55 parts of benevolence, 20-55 parts of lily, 20-55 parts of orange peel, 30-55 parts of rhizoma polygonati, 20-55 parts of cape jasmine, 20-55 parts of carrot, hard flower
20-75 parts of wild cabbage, 20-50 parts of germanium-enriched barley seedling, 20-50 parts of selenium-rich barley seedling.
2. adjusting the special dietary seafood of patient's immunocyte balance during a kind of chemicotherapy according to claim 1, feature exists
In: parts by weight of raw materials ratio range is:
90-270 parts of barley seedling, 90-150 parts of green tea, 50-130 parts of mulberry leaf, 70-120 parts of lotus leaf, 80-240 parts of honeysuckle, radix polygonati officinalis
15-25 parts, 42-50 parts of chrysanthemum, 36-42 parts of fructus lycii, 36-42 parts of cordate houttuynia, 36-50 parts of dandelion, 26-50 parts of lemon, coix lacryma-jobi
46-52 parts of benevolence, 30-50 parts of lily, 26-50 parts of orange peel, 36-48 parts of rhizoma polygonati, 26-48 parts of cape jasmine, 30-48 parts of carrot, hard flower
40-68 parts of wild cabbage, 30-40 parts of germanium-enriched barley seedling, 30-40 parts of selenium-rich barley seedling.
3. adjusting the special dietary seafood of patient's immunocyte balance, feature during a kind of chemicotherapy according to claim 1 or claim 2
Be: parts by weight of raw materials proportion is:
180 parts of barley seedling, 130 parts of green tea, 75 parts of mulberry leaf, 95 parts of lotus leaf, 125 parts of honeysuckle, 23 parts of radix polygonati officinalis, 48 parts of chrysanthemum, Chinese holly
39 parts of Qi, 38 parts of cordate houttuynia, 42 parts of dandelion, 43 parts of lemon, 48 parts of semen coicis, 40 parts of lily, 38 parts of orange peel, 42 parts of rhizoma polygonati,
38 parts of cape jasmine, 38 parts of carrot, 50 parts of curd, 36 parts of germanium-enriched barley seedling, 36 parts of selenium-rich barley seedling.
4. adjusting the special dietary seafood of patient's immunocyte balance, feature during a kind of chemicotherapy according to claim 1 or claim 2
Be: Ultramicro-powder, tablet or capsule is made in spy's dietary seafood.
5. adjusting the special dietary seafood of patient's immunocyte balance during a kind of chemicotherapy according to claim 3, feature exists
In: Ultramicro-powder, tablet or capsule is made in spy's dietary seafood.
6. adjusting the special dietary seafood of patient's immunocyte balance during a kind of chemicotherapy according to claim 4, feature exists
In: the Ultramicro-powder refers to that granularity is the particle of 200-500 mesh.
7. adjusting the special dietary seafood of patient's immunocyte balance, feature during a kind of chemicotherapy according to claim 1 or claim 2
Be: the application method of spy's dietary seafood is: 3 days before chemicotherapy start to eat, or take with Miles' operation, take daily
5-50g, until the chemicotherapy course for the treatment of terminates.
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