CN104474219A - Drug for treating trionyx sinensis saprolegniasis caused by fungi - Google Patents

Drug for treating trionyx sinensis saprolegniasis caused by fungi Download PDF

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CN104474219A
CN104474219A CN201410609549.0A CN201410609549A CN104474219A CN 104474219 A CN104474219 A CN 104474219A CN 201410609549 A CN201410609549 A CN 201410609549A CN 104474219 A CN104474219 A CN 104474219A
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parts
fructus
vitamin
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trionyx sinensis
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占水秀
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Abstract

The invention discloses a drug for treating trionyx sinensis saprolegniasis caused by fungi, wherein the drug comprises gentian violet, sodium chloride, honeysuckle, cnidium monnieri, wild chrysanthemum, acorus calamus, garlic, tangerine peel, and rhus chinensis. The drug of the present invention provides the inhibition effect on saprolegniaceae fungi and can be used for treating trionyx sinensis saprolegniasis. In addition, the invention further discloses a preparation method of the drug.

Description

Treat the medicine of fungus-caused Trionyx sinensis Wiegmann fish molds
Technical field
The present invention relates to the medicine relating to and treat Trionyx sinensis Wiegmann fish molds, it contains Chinese medicine ingredients.
Background technology
Fish molds is identical with leukoderma, and be that a kind of parasitic fungi is sick, its pathogenic bacterium are Saprolegnia and the Achyla of Saprolegniaceae.Less as the medicine for the treatment of fish molds disclosed in patent documentation, because same antibiotic substance to cause a disease original similar effect to a class, so, usually similar therapeutic scheme is adopted to mycosis." present situation of trionyx disease research and prospect thereof ", " Prevention Technique of common diseases of soft-shelled turtle ", " brief talking the medication problem in foster Trionyx sinensis Wiegmann process ", " wideling popularize application Chinese herbal medicine to prevent trionyx disease " etc. make introduction to the cause of disease of fish molds etc. and medication.201210272593.8 breeding methods relating to Trionyx sinensis Wiegmann, it discloses cod-liver oil, VE, VC, compound vitamin B, can promote that Trionyx sinensis Wiegmann Seedling is to the resistance of fungus.In aquaculture, generally do not advise adopting single Drug therapy disease, this easily causes Drug resistance, can make the enrichment of Trionyx sinensis Wiegmann vivotoxin simultaneously.Compound recipe is better selection, but how compatibility improves curative effect.It is a considerable problem.
Summary of the invention
The present invention proposes a kind of medicine for the treatment of Trionyx sinensis Wiegmann fish molds, and it can suppress the breeding of water mo(u)ld and continuous mycete, for Trionyx sinensis Wiegmann fish molds, and Be very effective.
For solving the problems of the technologies described above, the present invention constructs following technical proposals:
A kind of medicine for the treatment of Trionyx sinensis Wiegmann fish molds, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 12 parts, Gentian Violet 12 parts, Fructus Forsythiae 12 parts, 10 parts, sodium chloride, Herba Houttuyniae 10 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Rhizoma Belamcandae 9 parts, Fructus Xanthii 9 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, 9 parts, Bulbus Allii, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, sodium bicarbonate 5 parts, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B 21 part.
A kind of medicine for the treatment of Trionyx sinensis Wiegmann fish molds, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 10 parts, Gentian Violet 10 parts, Fructus Forsythiae 10 parts, Rhizoma Belamcandae 10 parts, sodium bicarbonate 10 parts, 10 parts, sodium chloride, Herba Houttuyniae 10 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Fructus Xanthii 9 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, 9 parts, Bulbus Allii, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B 21 part.
A kind of medicine for the treatment of Trionyx sinensis Wiegmann fish molds, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 15 parts, 15 parts, Bulbus Allii, Gentian Violet 15 parts, Fructus Forsythiae 15 parts, Rhizoma Belamcandae 15 parts, Herba Houttuyniae 15 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Fructus Xanthii 10 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, sodium bicarbonate 5 parts, 5 parts, sodium chloride, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B 21 part.
Treat a preparation method for the medicine of Trionyx sinensis Wiegmann fish molds, it is characterized in that comprising the following steps:
Pulverized by the sodium chloride of Pericarpium Citri Reticulatae, Fructus Crataegi and half, add the water of 2 times of quality, 2h at a simmer, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor;
By Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici, Cortex Phellodendri mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid;
By Radix Sophorae Flavescentis, Gentian Violet, Fructus Forsythiae, Rhizoma Belamcandae, Fructus Cnidii, sodium bicarbonate, Fructus Xanthii, Rhizoma Acori Graminei, Rhizoma Osmundae, Galla Chinensis, Radix Glycyrrhizae, Cortex Fraxini, malachite green oxalate mixing, add the water of twice quality, be warming up to 60 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 30min, filter, obtain filtrate;
Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, adds the water of vitamin C, malachite green oxalate, vitamin E, vitamin B2, residue sodium chloride and 4 times of quality, namely make described medicine.
Medicine of the present invention has restraint to Saprolegniaceae fungus, may be used for treatment Trionyx sinensis Wiegmann fish molds.Reasonable compatibility, Pericarpium Citri Reticulatae, Fructus Crataegi, Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici, Cortex Phellodendri etc. eliminate the heavy taste of Bulbus Allii at antifungal simultaneously.Radix Glycyrrhizae also can hepatoprotective, prevents the tannin injury hepatocyte of Galla Chinensis etc.Preparation method can improve effective component extraction rate, and reduces the loss of benefit materials.Bulbus Allii first mixes with Pericarpium Citri Reticulatae etc., de-tastes in advance.
Detailed description of the invention
Below provide some embodiments, and record its curative effect, the item do not described in detail in embodiment is see the common process in Chemistry for Chinese Traditional Medicine field, and detailed pharmacological action can see " Chinese Pharmacopoeia (2010 editions) ".
Embodiment one
The medicine for the treatment of Trionyx sinensis Wiegmann fish molds, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 12 parts, Gentian Violet 12 parts, Fructus Forsythiae 12 parts, 10 parts, sodium chloride, Herba Houttuyniae 10 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Rhizoma Belamcandae 9 parts, Fructus Xanthii 9 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, 9 parts, Bulbus Allii, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, sodium bicarbonate 5 parts, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B2 1 part.
Embodiment two
The medicine for the treatment of Trionyx sinensis Wiegmann fish molds, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 10 parts, Gentian Violet 10 parts, Fructus Forsythiae 10 parts, Rhizoma Belamcandae 10 parts, sodium bicarbonate 10 parts, 10 parts, sodium chloride, Herba Houttuyniae 10 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Fructus Xanthii 9 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, 9 parts, Bulbus Allii, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B2 1 part.
Embodiment three
The medicine for the treatment of Trionyx sinensis Wiegmann fish molds, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 15 parts, 15 parts, Bulbus Allii, Gentian Violet 15 parts, Fructus Forsythiae 15 parts, Rhizoma Belamcandae 15 parts, Herba Houttuyniae 15 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Fructus Xanthii 10 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, sodium bicarbonate 5 parts, 5 parts, sodium chloride, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B2 1 part.
Preparation method comprises the following steps:
Pulverized by the sodium chloride of Pericarpium Citri Reticulatae, Fructus Crataegi and half, add the water of 2 times of quality, 2h at a simmer, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor; By Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici, Cortex Phellodendri mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid; By Radix Sophorae Flavescentis, Gentian Violet, Fructus Forsythiae, Rhizoma Belamcandae, Fructus Cnidii, sodium bicarbonate, Fructus Xanthii, Rhizoma Acori Graminei, Rhizoma Osmundae, Galla Chinensis, Radix Glycyrrhizae, Cortex Fraxini, malachite green oxalate mixing, add the water of twice quality, be warming up to 60 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 30min, filter, obtain filtrate; Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, adds the water of vitamin C, malachite green oxalate, vitamin E, vitamin B2, residue sodium chloride and 4 times of quality, namely make described medicine.
Experiment one
Test method:
1, plant is divided into the test tank of equal-volume (50cm × 50cm × 40cm), is labeled as 30 groups according to Chinese medicine ingredients.Often group adopts same water source (tap water after lake water or aeration), without being communicated with between group.Water temperature 20 to 28 DEG C, pH=6-8.
2, get Trionyx sinensis Wiegmann 600 tail at random in ill plant, be placed in test tank by 20 tails/component.Every day throws in bait by the 3-4% of Trionyx sinensis Wiegmann body weight, and 8: 00,13: 00,16: 00 respectively throw something and feed once.Every day puts half water from bottom, injects water source to 60 ± 10cm simultaneously.
3, daily check, takes out dead or is about to dead Trionyx sinensis Wiegmann, in order to avoid cross infection, affecting result of the test.
Experimental Comparison:
Different Chinese herbal medicine decoctions is thrown according to group name.According to reality survival number, delimit the effect of the monomer in treating skin fester disease of each Chinese medicine ingredients as following table: it is better that "+" represents curative effect more.
Through test, the present invention chooses some drugs, simultaneously proportion optimizing.Bulbus Allii plays therapeutic effect, and Flos Chrysanthemi Indici, Flos Lonicerae, Fructus Crataegi, Pericarpium Citri Reticulatae etc. de-taste.
Test two
Test material and environment: test Trionyx sinensis Wiegmann derives from breeding soft-shell turtle field, Duchang city of Jiangxi Province, and the gross area is about 980m 2, total Trionyx sinensis Wiegmann number about 30000,2013, there is fish molds in certain culture zone of this plant, Trionyx sinensis Wiegmann is sporadicly dead.Trionyx sinensis Wiegmann test site is located at Duchang County, Jiangxi Province, is less than 10km apart from poyang lake water, and environment is suitable for Trionyx sinensis Wiegmann existence.Between test period March to April, cultivation 30d.
Test method:
1, plant is divided into five groups of test tanks of equal-volume (300cm × 300cm × 80cm), is labeled as A experimental group, B experimental group, C experimental group, A matched group, B matched group respectively.Often group adopts same water source (tap water after lake water or aeration), without being communicated with between group.Water temperature 20 to 28 DEG C, pH=6-8.
2, get test Trionyx sinensis Wiegmann 500 tail, be placed in five groups of test tanks by 100 tails/component.Every day throws in fish material by the 3-4% of Trionyx sinensis Wiegmann body weight, and 8: 00,13: 00,16: 00 respectively throw something and feed once.Every day puts half water from bottom, injects water source to 60 ± 10cm simultaneously.
3, daily check, finds (sensory evaluation, the statistical significance) of disease symptoms, takes out or isolation, in order to avoid cross infection, affects result of the test.
Experimental Comparison:
1, build cm × 80cm medicine pond, five group of 300 cm × 300, be labeled as A experimental group, B experimental group, C experimental group, A matched group, B matched group respectively.
2, medicine of the present invention is injected respectively the medicine pond of A experimental group, B experimental group, C experimental group, the degree of depth 30 ± 5 cm.Sodium bicarbonate water, saline solution inject the medicine pond of A matched group, and B matched group only injects water (or the tap water after lake water or aeration).
3, every 3 days by Trionyx sinensis Wiegmann take out, correspondence put into medicine pond soak 10 to 20 minutes, each medicine pond single only puts into a tail, after Orthogonal Rotational Regressive Tests pond.
Along with the passing of culturing time, in test tank, dead Trionyx sinensis Wiegmann mantissa is as following table:
Single soda water, Sal are only as normal sterile composition, and early stage is good to bacteriostatic ability, but the later stage is weak.Medicine of the present invention can play certain inhibition early stage, and the later stage can play therapeutical effect, eliminates the threat of fish molds completely.Bulbus Allii sterilization good medicine, but taste weight, easily injury Trionyx sinensis Wiegmann respiratory system and nervous system, the medicines such as compatibility Pericarpium Citri Reticulatae and Fructus Crataegi, removing abnormal flavour.In addition, Galla Chinensis etc. have injury to liver, and the liver protection effect of Radix Glycyrrhizae etc. is also this prescription benefit.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (5)

1. treat a medicine for Trionyx sinensis Wiegmann fish molds, it is characterized in that comprising: Gentian Violet, sodium chloride, Flos Lonicerae, Fructus Cnidii, Flos Chrysanthemi Indici, Rhizoma Acori Graminei, Bulbus Allii, Pericarpium Citri Reticulatae, Galla Chinensis.
2. the medicine for the treatment of Trionyx sinensis Wiegmann fish molds according to claim 1, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 12 parts, Gentian Violet 12 parts, Fructus Forsythiae 12 parts, 10 parts, sodium chloride, Herba Houttuyniae 10 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Rhizoma Belamcandae 9 parts, Fructus Xanthii 9 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, 9 parts, Bulbus Allii, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, sodium bicarbonate 5 parts, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B 21 part.
3. the medicine for the treatment of Trionyx sinensis Wiegmann fish molds according to claim 1, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 10 parts, Gentian Violet 10 parts, Fructus Forsythiae 10 parts, Rhizoma Belamcandae 10 parts, sodium bicarbonate 10 parts, 10 parts, sodium chloride, Herba Houttuyniae 10 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Fructus Xanthii 9 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, 9 parts, Bulbus Allii, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B 21 part.
4. the medicine for the treatment of Trionyx sinensis Wiegmann fish molds according to claim 1, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Sophorae Flavescentis 15 parts, 15 parts, Bulbus Allii, Gentian Violet 15 parts, Fructus Forsythiae 15 parts, Rhizoma Belamcandae 15 parts, Herba Houttuyniae 15 parts, Flos Lonicerae 10 parts, Cortex Phellodendri 10 parts, Fructus Cnidii 10 parts, Fructus Xanthii 10 parts, Flos Chrysanthemi Indici 9 parts, Rhizoma Acori Graminei 9 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Rhizoma Osmundae 6 parts, Galla Chinensis 6 parts, 6 parts, Radix Glycyrrhizae, Cortex Fraxini 6 parts, sodium bicarbonate 5 parts, 5 parts, sodium chloride, malachite green oxalate 2 parts, Catergen part, vitamin e1 part, vitamin B 21 part.
5. treat a preparation method for the medicine of Trionyx sinensis Wiegmann fish molds, it is characterized in that comprising the following steps:
Pulverized by the sodium chloride of Pericarpium Citri Reticulatae, Fructus Crataegi and half, add the water of 2 times of quality, 2h at a simmer, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor;
By Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici, Cortex Phellodendri mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid;
By Radix Sophorae Flavescentis, Gentian Violet, Fructus Forsythiae, Rhizoma Belamcandae, Fructus Cnidii, sodium bicarbonate, Fructus Xanthii, Rhizoma Acori Graminei, Rhizoma Osmundae, Galla Chinensis, Radix Glycyrrhizae, Cortex Fraxini, malachite green oxalate mixing, add the water of twice quality, be warming up to 60 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 30min, filter, obtain filtrate;
Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, adds the water of vitamin C, malachite green oxalate, vitamin E, vitamin B2, residue sodium chloride and 4 times of quality, namely make described medicine.
CN201410609549.0A 2014-11-04 2014-11-04 Drug for treating trionyx sinensis saprolegniasis caused by fungi Pending CN104474219A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104886388A (en) * 2015-05-27 2015-09-09 石东秀 Feed for treating small water turtle saprolegniasis and preparation method thereof
CN106421319A (en) * 2016-10-26 2017-02-22 临湘市东昇生物科技有限公司 Chinese herbal medicine for treating fungal diseases of aquatic animals and preparation method thereof
CN108888699A (en) * 2018-07-25 2018-11-27 贵州贵丰农业发展有限公司 A method of prevention and treatment soft-shelled turtle saprolegniasis
CN112691184A (en) * 2020-12-31 2021-04-23 西藏自治区农牧科学院水产科学研究所 Bacteriostatic composition for inhibiting saprolegnia and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104886388A (en) * 2015-05-27 2015-09-09 石东秀 Feed for treating small water turtle saprolegniasis and preparation method thereof
CN106421319A (en) * 2016-10-26 2017-02-22 临湘市东昇生物科技有限公司 Chinese herbal medicine for treating fungal diseases of aquatic animals and preparation method thereof
CN106421319B (en) * 2016-10-26 2018-01-09 临湘市东昇生物科技有限公司 A kind of aquatic products Chinese herbal medicine for the treatment of fungal disease and preparation method thereof
CN108888699A (en) * 2018-07-25 2018-11-27 贵州贵丰农业发展有限公司 A method of prevention and treatment soft-shelled turtle saprolegniasis
CN112691184A (en) * 2020-12-31 2021-04-23 西藏自治区农牧科学院水产科学研究所 Bacteriostatic composition for inhibiting saprolegnia and preparation method thereof

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Application publication date: 20150401