CN103349747B - Traditional Chinese medicine preparation for preventing and curing paralichthys olivaceus and sea cucumber scuticoci liatlda diseases - Google Patents
Traditional Chinese medicine preparation for preventing and curing paralichthys olivaceus and sea cucumber scuticoci liatlda diseases Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine preparation for preventing and curing paralichthys olivaceus and sea cucumber scuticoci liatlda diseases. The preparation comprises the following traditional Chinese medicine components in percentage by weight: 5-20 percent of abrotanum, 5-20 percent of azedarach, 5-20 percent of cyrtomium fortunei, 5-40 percent of stemona, 5-20 percent of golden cypress, 5-20 percent of betel nut and 5-30 percent of omphalia, and the sum of the weight percentages are 100. According to the invention, medicines with components restraining or killing pathogens are selected to make a formula on the basis of traditional Chinese theories, breeding bait is added and absorbed by the host of a parasite, scuticoci liatldas parasitizing in the body or on the body surface of the host of the parasite are completely killed in a body fluid absorbing and supplying way, so that the traditional Chinese medicine preparation has the advantages that medicine feeding is simple, the medicine effect is quick, the insert killing effect is good, the recovery of the physique of the host of the parasite is quick, the survival rate is high, side effects and medicine residue are avoided, as a result, economical loss caused by scuticoci liatlda infection during the production processes of breeding and growing.
Description
Technical field
The present invention relates to a kind of medicament preparation, especially a kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease.
Background technology
In recent years, along with the continuous expansion of cultivation scale, the continuous increase of region cultivation density, no matter breed variety is in the seed rearing stage, or in forming process, the farming disease harms happens occasionally, the harm of parasite type disease that the shield ciliate of especially take is main infection source is quite large, has had a strong impact on the survival rate of Cultured Flounder, Paralichthys Olivaceus fish, Radix Morinae Bulleyanae, it is one of comparatively serious disease of breeding production harm, also the quality that has affected cultivation object, causes production cost to increase simultaneously, and economic benefit is impaired.
Shield ciliate is named again and is had a liking for rotten worm, the shield balantidiasis infecting is commonly referred to balantidiasis, this disease sickness rate is high, and infection velocity is fast, and the popular time is long, often and other bacterial diseasees concurrent, can colonize in the body surface of fish, ginseng, also can parasitism in vivo, especially colonize in brain, spleen, kidney, liver and the blood of fish, also there is no at present specific short, can cause the large quantities of death of the infected.In addition, Radix Morinae Bulleyanae also can be often infected in the seed rearing stage, joined ingestion ability decline by parasitic children, stops gradually ingesting, and the final large skin death that quantizes, infects other individualities then, causes full pond dead.
In recent years, world biological person, exploring widely aspect the prevention of this disease and treatment, has obtained some comparatively desirable effects, has researched and developed multiple therapy methods.As adopted, change breeding water body salinity, regulate the method for osmotic pressure to embathe cultivation object and reach and remove ectozoal object; Adopt the different ions such as copper, zinc, hydrargyrum to embathe debug method; Hydrogen peroxide bathing debug method, formalin debug method and take to heat up, the multiple method of preventing and treating such as cooling debug method all obtains extensive and long-term application in breeding production, but production practices prove, adopt above-mentioned technology prevention method can only obtain the therapeutic effect of short time, very difficult radical cure more can not be killed parasitic ciliate in vivo, and cause drug residue, so in breeding production, this disease still can cause very large economic loss.
Summary of the invention
In order to overcome applied various Prevention Technique in prior art, are the methods from outer treatment, can only kill except the shield ciliate that colonizes in body surface, and relapse rate is high, the probability of repeated infection is large, and cannot kill parasitic ciliophoran deficiency in vivo, the invention provides a kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease.This Chinese medicine preparation does not affect cultivation object and ingests and activity, do not affect its growth, and to parasitism in vivo, the shield Infusoria of body surface has good killing action, good disinsection effect, it is fast that host's body constitution is recovered, survival rate is high, can greatly reduce in cultivation, the production process of growing seedlings because shield ciliate infects the economic loss causing.
The technical solution adopted for the present invention to solve the technical problems is: a kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, it is characterized in that: be to pulverize and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 5-20%, Cortex Meliae 5-20%, Rhizoma Osmundae 5-20%, Radix Stemonae 5-40%, Cortex Phellodendri 5-20%, Semen Arecae 5-20% and Omphalia 5-30%, its weight percentages of components sum is 100%.
The consumption of described Chinese medicine preparation is 3 ‰ of breeding bait weight-8 ‰.
The selection of Chinese medicine preparation of the present invention and prescription are to select there is the medicine that suppresses or kill pathogen composition according to tcm theory, in this Chinese medicine preparation: Herba Artemisiae Annuae bitter in the mouth, property are pungent cold, have clearind deficient heat, separate summer-heat, the effect of preventing the attack (or recurrence) of malaria; Modern study shows: Herba Artemisiae Annuae mainly contains sesquiterpenoids, flavonoid, Coumarins, volatile ingredient and other beta galactosidases, beta-glucosidase, cupreol etc., and wherein sesquiterpenoids has arteannuin, artelinic acid, artemisinol, methyl arteannuate etc.; Its arteannuin and derivant have the bilharzial effect of anti-animal; Arteannuin, SM-224, artesunate all can promote the immunization of body cell; Herba Artemisiae Annuae decocting liquid has lethal effect to various bacteria, virus.Cortex Meliae bitter in the mouth, cold in nature, has parasite killing, treats the effect of tinea; Modern study shows: Cortex Meliae contains Azedarachin, Cen skin ketone, bitter lactone, melianotriol, positive state alkane, cupreol, Fructus Toosendan ketone etc.; Contained main component Azedarachin, drives ascarid effect, and its melianol immersion or water extract have obvious inhibitory action to common pathogenic fungus.Rhizoma Osmundae bitter in the mouth, cold nature, have heat-clearing and toxic substances removing, the effect of hemostasis parasite killing; Modern study shows: Rhizoma Osmundae main component is pannol, is a kind of phloroglucinol derivant of complexity, can slowly decompose and produces albaspidin and white albaspidin; Again containing aspidinol, flavaspidic acid, filicinic acid; Separately contain volatilization sleeve, filicitannic acid, fat, resin etc.; Still containing triterpenoid fernene, jasmine alkene-b, adianton, jasmine alcohol, jasmine alcohol-29 wild goose tooth alkene etc.; Its contained Filixic Acids, flavaspidic acid have stronger anthelmintic action, and cestode is had to strong toxicity, can make cestode benumb and discharge, and also have the parasitic effects such as the ancylostome of expeling, ascarid; Rhizoma Osmundae has obvious inhibitory action to multiple Causative virus, fungus.Radix Stemonae sweet in the mouth, hardship, slightly warm in nature, has the effect of nourishing the lung to keep the adverse QI downward, parasite killing delousing; Modern study shows: the Radix Stemonae is containing multiple alkaloid: as (-)-Stemoninine, Stemonidine, protostermonine, inferior (-)-Stemoninine, sessilistemonine, tuberostemonine, stemonamine etc., and also sugary, lipid, protein and acetic acid, formic acid, malic acid, succinic acid, oxalic acid etc.Its melianol immersion or water extract to various pathogens as streptococcus pneumoniae, beta hemolysis type streptococcus, meningococcus, staphylococcus aureus, Staphylococcus albus and dysentery bacterium, Bacillus typhi, Salmonella paratyphi, escherichia coli, Bacillus proteus, diphtheria corynebacterium, pneumobacillus, bacillus pestis, anthrax bacillus, bacillus subtilis, and vibrio cholera, Bacillus tuberculosis etc. have bacteriostasis in various degree; Some pathomycete is had to certain inhibitory action; In addition, its infusion and ethanol immersion all have killing action to mosquitos and flies larva, head louse, clothing louse and Cimex bedbug etc.Cortex Phellodendri bitter in the mouth, cold in nature, there is heat clearing and damp drying, pathogenic fire purging except steaming, detoxification sore treatment effect; Modern study shows: Cortex Phellodendri bark is containing alkaloids such as berberine, jateorhizine, magnoflorine, phellodendrine, candicine, palmatine, Dauricines; Another containing obacunone, obakulactone, Dictamnolide, obacunonic acid, the acid of blue or green fluorescent, 7-dehydrogenation stigmasterol, cupreol, campesterol.Cortex Phellodendri has resisting pathogenic microbes effect, and the multiple malignant bacterias such as dysentery bacterium, Bacillus typhi, staphylococcus aureus, Hemolytic streptococcus are all had to inhibitory action; Its decoct or preserved material have bacteriostasis in various degree to some common pathogenic funguses.Semen Arecae bitter in the mouth, property are pungent, have the effect of killing insect and eliminating accumulation, promoting the circulation of QI to induce diuresis, preventing the attack (or recurrence) of malaria; Modern study shows: Semen Arecae contains alkaloid 0.3～0.6%, condensed tannin 15%, fat 14% and areca red.Alkaloid master is arecoline, content 0.1～0.5%; All the other have arecaidine, demethyl arecaidine, guvacoline, arecolidine, homarecoline etc.; Bulky alkali is effective anthelmintic ingredient, and cestode, pinworm, ascarid, ancylostome, schistosomicide etc. are all had paralysis or kill effect; Its contained tannin is to all having inhibitory action to multiple Causative virus, fungus.The selected crude drug of the present invention to cultivation object without noci-influence, the effect not only with parasite killing, anthelmintic, and there is hemopoietic or promote effect and the hepatic cholagogic that body fluid circulates, stomach invigorating is eaten by force, strengthening vital QI to eliminate pathogenic factors, health invigorating and scalable glandular secretion, can improve cultivation object autoimmune function.Omphalia is the bacterium sclerotium of Polyporaceae plant Omphalia lapidescens, and its mildly bitter flavor is cold in nature, has the effect of killing insect and eliminating accumulation; Modern study shows: main component is that a kind of protease claims Omphalin, and content approximately 3%, for expelling tenia effective ingredient, adds thermal failure; The expelling tenia effect of Omphalia is not paralysis polypide, but due to the decomposition of the protease in Omphalia to protein, destroys lethal the dying of worm joint.
Chinese medicine preparation of the present invention is to add in breeding bait, host ingest by the absorption of body fluid and supply method thoroughly kill parasitic in vivo with the shield ciliate of body surface, not affecting cultivation object ingests and activity, do not affect its growth, a kind of raising host autoimmunity, the Chinese medicine preparation to internal therapy.The Chinese medicine preparation of this control Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, administration is simple to operate, drug effect is fast, good disinsection effect, it is fast that host's body constitution is recovered, and input cost is low, survival rate is high, have no side effect, drug residue free, can reduce in cultivation, the production process of growing seedlings greatly because shield ciliate infects the economic loss causing.
The specific embodiment
Below in conjunction with embodiment and effect experiment, the present invention is described in further details.
Embodiment
1
A kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, is to be pulverized and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 5%, Cortex Meliae 15%, Rhizoma Osmundae 20%, the Radix Stemonae 10%, Cortex Phellodendri 5%, Semen Arecae 20% and Omphalia 25%.
Embodiment
2
A kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, is to be pulverized and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 15%, Cortex Meliae 20%, Rhizoma Osmundae 5%, the Radix Stemonae 15%, Cortex Phellodendri 15%, Semen Arecae 15% and Omphalia 15%.
Embodiment
3
onethe Chinese medicine preparation of planting control Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, is to be pulverized and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 20%, Cortex Meliae 5%, Rhizoma Osmundae 15%, the Radix Stemonae 5%, Cortex Phellodendri 20%, Semen Arecae 5% and Omphalia 30%.
Embodiment
4
oneplant the Chinese medicine preparation of control Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, is to be pulverized and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 12%, Cortex Meliae 12%, Rhizoma Osmundae 8%, the Radix Stemonae 40%, Cortex Phellodendri 13%, Semen Arecae 10% and Omphalia 5%.
Embodiment
5
A kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, is to be pulverized and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 10%, Cortex Meliae are 10%, Rhizoma Osmundae is 10%, the Radix Stemonae is 30%, Cortex Phellodendri is 10%, Semen Arecae is 10% and Omphalia 20%.
In above-described embodiment, be to take respectively raw material of Chinese medicine Herba Artemisiae Annuae, Cortex Meliae, Rhizoma Osmundae, the Radix Stemonae, Cortex Phellodendri, Semen Arecae, Omphalia, these several raw material of Chinese medicine are broken into fine powder through superfine powder, cross after 150 order-200 mesh sieves, packed sealing after abundant mix homogeneously.During use, Chinese medicine preparation is added in breeding bait, fully mix mixing thoroughly, or with clean water, be diluted to juice and with miniaturised nebuliser, be evenly sprayed at feedstuff surface, the use of throwing something and feeding after drying a little.
A Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, its effect experiment is as follows:
One, the effect experiment of control Paralichthys olivaceus shield ciliate disease
1, prepare test net cage: in ciliate spot, first select 3 net cages that morbidity is serious, concentrate morbidity Paralichthys olivaceus in two net cages wherein.
2, test fish specification and density: specification 15-25cm of morbidity fish, each proof box 500 tail.
3, administration time and administrated method: with 3 ‰ and 10 ‰ medication amount, the two casees test fishes of throwing something and feeding, take medicine bait twice every day respectively, throws once morning, and throwing at dusk once.Throw something and feed continuously 20 days.
4, result of the test: in this test originally, ingest normally with the test fish of 3 ‰ dosages, and tell food phenomenon with the fish body in the proof box of 10 ‰ dosages.Next day, two kinds of administration ratios being passed to tune separately up and down, soon in 3 ‰ dosage, be adjusted at 9 ‰ o'clock, there is telling food phenomenon in indivedual fish bodies; 10 ‰ ratio is adjusted downward to at 8 ‰ o'clock, and fish body is ingested normally, does not tell food phenomenon.For this reason, the scope for the treatment of dosage is defined as to 3 ‰-8 ‰, and continues administration with 8 ‰ ratio.Pharmaceutical chemistry is fed to field observation in the 7th day, and the sick fish state of two proof boxs is clearly better, and daily mortality amount declines day by day, and fish body enlivens, robs food, body colour is good, and fin launches, and body surface secretions obviously reduces, dead individual obvious decline.Medication 10 days, the state of an illness is substantially controlled.Medication 15 days, two test net cages do not have dead individual again.Medication 20 days, it is normal that fish body recovers, and respectively fishes for respectively 10 tail fishes, and microscopy body surface position and brain, blood and internal organs, all do not find ciliate.Sampling microscopy, equal fibre-less worm in body surface, body.Illustrate that this medicine has significant therapeutic effect to Paralichthys olivaceus shield ciliate.
Two, control Radix Morinae Bulleyanae shield ciliate disease effect experiment
1, choose the morbidity seedling in three ponds.
2, test sea cucumbers specification and density: morbidity seedling specification be 1000-500 jin.20 jin of every pond sea cucumbers gross weights.
3, administration time and administrated method: consider that sea cucumbers body is little enervated, with 3 ‰, 5 ‰, 8 ‰ the medication amount tested sick ginseng of throwing something and feeding respectively, every day, bait throwing in twice respectively, and throws once morning, and once, 5-7 days continuously throws something and feeds in throwing at dusk.
4, result of the test: the phenomenon that the same day of the 8 ‰ medication amount pharmaceutical chemistry of throwing something and feeding, sea cucumbers was assumed an air of complacency just obviously reduces, the throw something and feed second day of pharmaceutical chemistry, the sea cucumbers of changing skin obviously reduces, throw something and feed the 3rd day of pharmaceutical chemistry, sea cucumbers body surface by ciliate attack to destroy form the eyelet that festers, rotten point is repaired, the medicine to that continues to throw something and feed obtains thoroughly recovery from illness for 7 days.By observing, the throw something and feed corresponding prolongation of sea cucumbers cure time of 8 ‰ medication amount pharmaceutical chemistry of the sea cucumbers cure time of 5 ‰, 3 ‰ medication amount pharmaceutical chemistry of throwing something and feeding, but in the process of throwing something and feeding, the throw something and feed sea cucumbers mortality rate of 8 ‰ medication amount pharmaceutical chemistry of its sea cucumbers skin mortality rate is high, builds and adopts the treatment of throwing something and feeding of 5 ‰-8 ‰ medication amount pharmaceutical chemistry.
Through test determination: the Chinese medicine preparation of control Paralichthys olivaceus of the present invention, Radix Morinae Bulleyanae shield ciliate disease reaches 100% to effective prevention rate of Paralichthys olivaceus, Radix Morinae Bulleyanae ciliate disease, effectively cure rate reaches more than 87%.
Chinese medicine preparation medicine character of the present invention is stable, and nonirritant, has no side effect, and without residual in body, also there is no teratogenesis, a phenomenon that disables, and cultivation object, without nocuity, is had advantages of to application cost is low, preventive effect good, cure rate is high, easy to operation.In seawater seed rearing and intensive culture production, have broad application prospects.
Claims (2)
1. a Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease, this Chinese medicine preparation is powder, it is characterized in that: be to be pulverized and form by weight percentage by following traditional Chinese medicine raw: Herba Artemisiae Annuae 10%, Cortex Meliae 10%, Rhizoma Osmundae 10%, the Radix Stemonae 30%, Cortex Phellodendri 10%, Semen Arecae 10% and Omphalia 20%.
2. a kind of Chinese medicine preparation of preventing and treating Paralichthys olivaceus, Radix Morinae Bulleyanae shield ciliate disease according to claim 1, is characterized in that: the consumption of described Chinese medicine preparation is 3 ‰ of breeding bait weight-8 ‰.
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| CN104189313A (en) * | 2014-09-19 | 2014-12-10 | 山东新希望六和集团有限公司 | Medicinal composition for treating crucian carp ichthyoxeniosis and preparation method thereof |
| CN104367907B (en) * | 2014-11-27 | 2017-08-22 | 贵州省畜牧兽医研究所 | A kind of Chinese herbal medicine sheep tick insect resistant agent and its preparation and application method |
| CN104800469A (en) * | 2015-05-20 | 2015-07-29 | 山东亚康药业股份有限公司 | Traditional Chinese medicine preparation for treating pathogenesis of turbot of aquatic animals |
| CN105766719A (en) * | 2016-03-22 | 2016-07-20 | 五河县金塘水产养殖有限公司金贸园分公司 | Breeding method for preventing and controlling ciliophora disease of mandarin fish |
| CN105770438A (en) * | 2016-03-22 | 2016-07-20 | 五河县金塘水产养殖有限公司金贸园分公司 | Medicament composition for preventing and treating siniperca chuatsi ciliate |
| CN105918166A (en) * | 2016-04-25 | 2016-09-07 | 固镇县益民养殖专业合作社 | Breeding method used for preventing and treating Ciliophora disease of Siniperca chuatsi |
| CN106309866A (en) * | 2016-09-30 | 2017-01-11 | 威海虹润海洋科技有限公司 | Traditional Chinese medicine preparation for preventing and treating stichopus japonicas mouthpart fester diseases and skin ulcerative diseases and preparation method thereof |
| CN110075144B (en) * | 2019-06-19 | 2021-06-11 | 北京生泰尔科技股份有限公司 | Application of notopterygium root in preparing medicine for resisting scuticociliatida |
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