CN105316303B - 具有改善的特性的β-葡萄糖苷酶I变体 - Google Patents
具有改善的特性的β-葡萄糖苷酶I变体 Download PDFInfo
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
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| UA114276C2 (uk) | 2009-12-23 | 2017-05-25 | Даніско Юес Інк. | Спосіб одночасного оцукрювання і ферментації |
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| CN105886485B (zh) | 2010-10-01 | 2019-09-24 | 诺维信股份有限公司 | β-葡糖苷酶变体及其编码多核苷酸 |
| JPWO2012060389A1 (ja) * | 2010-11-05 | 2014-05-12 | 旭硝子株式会社 | シゾサッカロミセス属酵母の形質転換体およびその製造方法 |
| CN103842515A (zh) | 2011-03-17 | 2014-06-04 | 丹尼斯科美国公司 | 降低糖化过程的粘度的方法 |
| CN102816748B (zh) * | 2011-06-09 | 2014-07-16 | 中国科学院上海生命科学研究院 | 一种新型β-葡萄糖苷酶及其编码基因和应用 |
| ES2655051T3 (es) | 2011-08-26 | 2018-02-16 | Wisconsin Alumni Research Foundation | Virus de la gripe con segmento génico PB2 mutante como vacunas vivas atenuadas |
| BR112015012968A2 (pt) | 2012-12-07 | 2017-09-12 | Danisco Us Inc | composições e métodos de uso |
| ES2521940B1 (es) | 2013-05-10 | 2015-09-09 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Variantes de beta-glucosidasa con actividad de transglicosilación reducida |
| DK3027742T3 (da) | 2013-07-29 | 2020-02-24 | Danisco Us Inc | Enzymvarianter |
| US10077436B2 (en) | 2013-09-17 | 2018-09-18 | Edeniq, Inc. | Beta-glucosidase enzymes for increased biomass saccharification |
| US10982204B2 (en) * | 2014-02-26 | 2021-04-20 | Chr. Hansen A/S | Variants of chymosin with improved milk-clotting properties |
| US10053671B2 (en) | 2014-06-20 | 2018-08-21 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
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| CN107429267B (zh) * | 2014-12-19 | 2021-07-16 | 丹尼斯科美国公司 | 葡糖淀粉酶共混物 |
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| EP3387140A1 (en) * | 2015-12-10 | 2018-10-17 | DSM IP Assets B.V. | Beta-glucosidase and uses thereof |
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| EP3336180A1 (en) * | 2016-07-04 | 2018-06-20 | Evonik Degussa GmbH | Mutant alkb gene |
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| KR102593668B1 (ko) | 2016-10-04 | 2023-10-24 | 다니스코 유에스 인크. | 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산 |
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| MX2019007335A (es) | 2016-12-21 | 2019-09-09 | Dupont Nutrition Biosci Aps | Métodos para usar serina proteasas termoestables. |
| BR112019018381A2 (pt) | 2017-03-06 | 2020-04-07 | Dupont Nutrition Biosci Aps | fucosidases fúngicas e seu uso na prevenção e/ou no tratamento de uma infecção patogênica em um animal |
| WO2018213482A1 (en) * | 2017-05-16 | 2018-11-22 | The Curators Of The University Of Missouri | Immobilized enzyme complexes and related methods |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
| JP7051491B2 (ja) | 2018-02-26 | 2022-04-11 | 花王株式会社 | 変異β－グルコシダーゼ |
| BR112020018294A2 (pt) | 2018-03-09 | 2020-12-29 | Danisco Us Inc. | Glucoamilases e métodos de uso dos mesmos |
| BR112021000059A2 (pt) | 2018-07-04 | 2021-04-06 | Danisco Us Inc. | Glucoamilases e métodos para uso das mesmas |
| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
| US11389523B2 (en) | 2018-08-20 | 2022-07-19 | Wisconsin Alumni Research Foundation (Warf) | Vectors for eliciting immune responses to non-dominant epitopes in the hemagglutinin (HA) protein |
| US12234464B2 (en) | 2018-11-09 | 2025-02-25 | Ginkgo Bioworks, Inc. | Biosynthesis of mogrosides |
| CA3120360A1 (en) | 2018-11-20 | 2020-05-28 | Dupont Nutrition Biosciences Aps | Engineered robust high tm-phytase clade polypeptides and fragments thereof |
| EP3914295A2 (en) * | 2019-01-23 | 2021-12-01 | Yoshihiro Kawaoka | Mutations that confer genetic stability to additional genes in influenza viruses |
| CN113874496A (zh) | 2019-02-08 | 2021-12-31 | 威斯康星校友研究基金会(Warf) | 人源化细胞系 |
| WO2020223699A1 (en) | 2019-05-01 | 2020-11-05 | Wisconsin Alumni Research Foundation (Warf) | Improved influenza virus replication for vaccine development |
| JP7627911B2 (ja) | 2019-08-27 | 2025-02-07 | ウィスコンシン アルムニ リサーチ ファンデイション | 卵内複製のための安定化されたｈａを持つ組換えインフルエンザウイルス |
| WO2021150874A1 (en) | 2020-01-24 | 2021-07-29 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized na |
| US20230240334A1 (en) | 2020-02-28 | 2023-08-03 | Dupont Nutrition Biosciences Aps | Feed compositions |
| WO2021195410A1 (en) | 2020-03-25 | 2021-09-30 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
| CN115478060B (zh) * | 2021-06-16 | 2023-11-28 | 弈柯莱生物科技(集团)股份有限公司 | 一种糖基转移酶及其应用 |
| CN113842454B (zh) * | 2021-09-30 | 2026-01-30 | 中国人民解放军空军军医大学 | 一种基于vsv载体的htnv疫苗及其制备方法和应用 |
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| CN118853624B (zh) * | 2024-09-29 | 2025-04-25 | 青岛奔月生物技术有限公司 | 催化莱鲍迪苷d生产莱鲍迪苷m的葡萄糖基转移酶突变体及其应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6103464A (en) * | 1990-12-10 | 2000-08-15 | Genencor International, Inc. | Method of detecting DNA encoding a β-glucosidase from a filamentous fungus |
| EP1225227A1 (en) * | 1990-12-10 | 2002-07-24 | Genencor International, Inc. | Improved saccharification of cellulose by cloning and amplification of the beta-glucosidase gene of trichoderma reesei |
| CN101287751A (zh) * | 2005-08-04 | 2008-10-15 | 诺维信股份有限公司 | 具有β-葡糖苷酶活性的多肽和编码该多肽的多核苷酸 |
Family Cites Families (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4822516A (en) | 1986-12-08 | 1989-04-18 | Kao Corporation | Detergent composition for clothing incorporating a cellulase |
| JP2728531B2 (ja) | 1988-03-24 | 1998-03-18 | ノボ ノルディスク アクティーゼルスカブ | セルラーゼ調製品 |
| US5776757A (en) | 1988-03-24 | 1998-07-07 | Novo Nordisk A/S | Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof |
| DK494089D0 (enExample) | 1989-10-06 | 1989-10-06 | Novo Nordisk As | |
| US5326477A (en) | 1990-05-07 | 1994-07-05 | Bio-Sep, Inc. | Process for digesting solid waste |
| DE69133352T2 (de) | 1990-10-05 | 2004-11-04 | Genencor International, Inc., Palo Alto | Methoden zur behandlung baumwolle-enthaltender fasern mit zellulase |
| US5475101A (en) | 1990-10-05 | 1995-12-12 | Genencor International, Inc. | DNA sequence encoding endoglucanase III cellulase |
| US5246853A (en) | 1990-10-05 | 1993-09-21 | Genencor International, Inc. | Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I |
| US6021536A (en) | 1993-03-04 | 2000-02-08 | Wasinger; Eric | Mechanical desizing and abrading device |
| FI932521A0 (fi) | 1993-06-02 | 1993-06-02 | Alko Ab Oy | Nytt endoglukanasenzym |
| US6426200B1 (en) | 1994-09-15 | 2002-07-30 | University Of Georgia Research Foundation, Inc. | Methods for enzymatic deinking of waste paper |
| WO1996023062A1 (en) | 1995-01-26 | 1996-08-01 | Novo Nordisk A/S | Animal feed additives comprising xylanase |
| AU6513096A (en) | 1995-07-19 | 1997-02-18 | Novo Nordisk A/S | Treatment of fabrics |
| JP3626203B2 (ja) | 1995-11-15 | 2005-03-02 | ノボザイムス アクティーゼルスカブ | 染色されたデニムの複合糊抜及び「ストーンウォッシュ」方法 |
| WO1997028256A1 (en) | 1996-01-29 | 1997-08-07 | Novo Nordisk A/S | Process for desizing cellulosic fabric |
| US6254722B1 (en) | 1996-03-27 | 2001-07-03 | North Carolina State University | Method for making dissolving pulp from paper products containing hardwood fibers |
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US6069122A (en) | 1997-06-16 | 2000-05-30 | The Procter & Gamble Company | Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution |
| US6265204B1 (en) | 1997-01-17 | 2001-07-24 | Genencor International, Inc. | DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi |
| AU7428398A (en) | 1997-04-07 | 1998-10-30 | Unilever Plc | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus aspergillus |
| WO1998056958A1 (en) | 1997-06-10 | 1998-12-17 | Xyrofin Oy | Process for the production of xylose from a paper-grade, hardwood pulp |
| DE69812403T2 (de) | 1997-11-10 | 2004-01-29 | Procter & Gamble | Verfahren zur herstellung einer waschmitteltablette |
| US6187580B1 (en) | 1997-11-24 | 2001-02-13 | Novo Nordisk A/S | Pectate lyases |
| AU3408199A (en) | 1998-05-01 | 1999-11-23 | Novo Nordisk A/S | Enhancers such as n-hydroxyacetanilide |
| DE19824705A1 (de) | 1998-06-03 | 1999-12-09 | Henkel Kgaa | Amylase und Protease enthaltende Wasch- und Reinigungsmittel |
| US5980581A (en) | 1998-09-08 | 1999-11-09 | The Virkler Company | Process for desizing and cleaning woven fabrics and garments |
| US6024766A (en) | 1999-01-27 | 2000-02-15 | Wasinger; Eric M. | Process for enzymatic desizing of garments and enzyme deactivation |
| US6982159B2 (en) | 2001-09-21 | 2006-01-03 | Genencor International, Inc. | Trichoderma β-glucosidase |
| US7005289B2 (en) | 2001-12-18 | 2006-02-28 | Genencor International, Inc. | BGL5 β-glucosidase and nucleic acids encoding the same |
| US7045332B2 (en) | 2001-12-18 | 2006-05-16 | Genencor International, Inc. | BGL4 β-glucosidase and nucleic acids encoding the same |
| WO2004099228A2 (en) | 2003-05-02 | 2004-11-18 | Novozymes Inc. | Variants of beta-glucosidases |
| US20060041961A1 (en) | 2004-03-25 | 2006-02-23 | Abad Mark S | Genes and uses for pant improvement |
| US7413887B2 (en) | 2004-05-27 | 2008-08-19 | Genecor International, Inc. | Trichoderma reesei glucoamylase and homologs thereof |
| US7998713B2 (en) | 2005-04-12 | 2011-08-16 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain ethanol |
| US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
| CA2698765A1 (en) | 2007-09-07 | 2009-03-19 | Danisco Us Inc. | Beta-glucosidase enhanced filamentous fungal whole cellulase compositions and methods of use |
| ES2504983T3 (es) | 2007-10-09 | 2014-10-09 | Danisco Us Inc. | Variantes de glucoamilasa |
| US10676751B2 (en) * | 2008-02-29 | 2020-06-09 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| MX2011002142A (es) * | 2008-08-29 | 2011-04-26 | Iogen Energy Corp | Beta-glucosidasas modificadas con estabilidad mejorada. |
| FR2935986B1 (fr) | 2008-09-12 | 2012-11-23 | Inst Francais Du Petrole | Variants de beta-glucosidase a activite amelioree et leurs utilisations. |
| CA2763836A1 (en) * | 2009-05-29 | 2010-12-02 | Iogen Energy Corporation | Novel beta-glucosidase enzymes |
| CN103842515A (zh) | 2011-03-17 | 2014-06-04 | 丹尼斯科美国公司 | 降低糖化过程的粘度的方法 |
| CA2830239A1 (en) | 2011-03-17 | 2012-09-20 | Danisco Us Inc. | Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis |
| US8468683B2 (en) * | 2011-10-31 | 2013-06-25 | HGST Netherlands B.V. | High bevel angle magnetic writer pole fabrication process |
| JP2015533294A (ja) | 2012-10-31 | 2015-11-24 | ダニスコ・ユーエス・インク | ニューロスポラ・クラッサ（Ｎｅｕｒｏｓｐｏｒａｃｒａｓｓａ）由来のβ−グルコシダーゼ |
| MX2015005427A (es) | 2012-10-31 | 2015-08-05 | Danisco Inc | Beta-glucosidasa de magnaporthe grisea. |
| WO2014070841A1 (en) | 2012-10-31 | 2014-05-08 | Danisco Us Inc. | Compositions and methods of use |
-
2010
- 2010-11-19 EP EP10782511.9A patent/EP2501806B1/en not_active Not-in-force
- 2010-11-19 EP EP12199327.3A patent/EP2599861B1/en not_active Not-in-force
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- 2010-11-19 EP EP12199329.9A patent/EP2599862B1/en not_active Not-in-force
- 2010-11-19 CA CA2780767A patent/CA2780767A1/en not_active Abandoned
- 2010-11-19 DK DK12199332.3T patent/DK2599864T3/da active
- 2010-11-19 EP EP12199328.1A patent/EP2602317B1/en not_active Not-in-force
- 2010-11-19 DK DK10782511.9T patent/DK2501806T3/da active
-
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- 2015-06-16 JP JP2015120937A patent/JP2015211678A/ja not_active Withdrawn
-
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- 2016-08-29 US US15/250,626 patent/US20170145396A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6103464A (en) * | 1990-12-10 | 2000-08-15 | Genencor International, Inc. | Method of detecting DNA encoding a β-glucosidase from a filamentous fungus |
| EP1225227A1 (en) * | 1990-12-10 | 2002-07-24 | Genencor International, Inc. | Improved saccharification of cellulose by cloning and amplification of the beta-glucosidase gene of trichoderma reesei |
| CN101287751A (zh) * | 2005-08-04 | 2008-10-15 | 诺维信股份有限公司 | 具有β-葡糖苷酶活性的多肽和编码该多肽的多核苷酸 |
Non-Patent Citations (3)
| Title |
|---|
| "DNA family shuffling of hyperthermostable β-glycosidases";Thijs Kaper et al.;《Biochem. J.》;20020806;第368卷;第461-470页 |
| "The bgl1 gene of Trichoderma reesei QM 9414 encodes an extracellular, cellulose-inducible p-glucosidase involved in cellulase induction by sophorose";Robert L.Mach et al.;《Molecuiar Microbioiogy》;19951231;第16卷(第4期);第687-697页 |
| "The Use of Cellulases from a β-Glucosidase-Hyperproducing Mutant of Trichoderma reesei in Simultaneous Saccharification and Fermentation of Wheat Straw";Janusz Szczodrak;《Biotechnology and Bioengineering》;19890430;第33卷;第1112-1116页 |
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