CN105316303B - 具有改善的特性的β-葡萄糖苷酶I变体 - Google Patents
具有改善的特性的β-葡萄糖苷酶I变体 Download PDFInfo
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
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| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
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| WO2012060389A1 (ja) * | 2010-11-05 | 2012-05-10 | 旭硝子株式会社 | シゾサッカロミセス属酵母の形質転換体およびその製造方法 |
| EP2686434B1 (en) | 2011-03-17 | 2021-07-14 | Danisco US Inc. | Method for reducing viscosity in saccharification process |
| CN102816748B (zh) * | 2011-06-09 | 2014-07-16 | 中国科学院上海生命科学研究院 | 一种新型β-葡萄糖苷酶及其编码基因和应用 |
| JP2014527799A (ja) | 2011-08-26 | 2014-10-23 | ダブリュエーアールエフ−ウィスコンシン アラムナイ リサーチ ファウンデーション | 変異型ｐｂ２遺伝子セグメントを有する弱毒化生ワクチンとしてのインフルエンザウイルス |
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| EP3027742B1 (en) | 2013-07-29 | 2019-11-20 | Danisco US Inc. | Variant enzymes |
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| CN113403357B (zh) * | 2014-12-19 | 2024-10-18 | 丹尼斯科美国公司 | 葡糖淀粉酶共混物 |
| US20180148683A1 (en) | 2015-07-07 | 2018-05-31 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| WO2017097897A1 (en) * | 2015-12-10 | 2017-06-15 | Dsm Ip Assets B.V. | Beta-glucosidase and uses thereof |
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| KR102593668B1 (ko) | 2016-10-04 | 2023-10-24 | 다니스코 유에스 인크. | 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산 |
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| RU2771261C2 (ru) | 2016-12-21 | 2022-04-29 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения термостабильных сериновых протеаз |
| EP3592429A1 (en) | 2017-03-06 | 2020-01-15 | DuPont Nutrition Biosciences ApS | Novel fungal fucosidases and their use in preventing and/or treating a pathogenic infection in an animal |
| WO2018213482A1 (en) * | 2017-05-16 | 2018-11-22 | The Curators Of The University Of Missouri | Immobilized enzyme complexes and related methods |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
| JP7051491B2 (ja) * | 2018-02-26 | 2022-04-11 | 花王株式会社 | 変異β－グルコシダーゼ |
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