JP2013511280A - 改良された特性を有するβ−グルコシダーゼＩ変異体 - Google Patents
改良された特性を有するβ−グルコシダーゼＩ変異体 Download PDFInfo
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- JP2013511280A JP2013511280A JP2012540120A JP2012540120A JP2013511280A JP 2013511280 A JP2013511280 A JP 2013511280A JP 2012540120 A JP2012540120 A JP 2012540120A JP 2012540120 A JP2012540120 A JP 2012540120A JP 2013511280 A JP2013511280 A JP 2013511280A
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- Prior art keywords
- bgl1
- mutant
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26324009P | 2009-11-20 | 2009-11-20 | |
| US61/263,240 | 2009-11-20 | ||
| PCT/US2010/057531 WO2011063308A2 (en) | 2009-11-20 | 2010-11-19 | Beta-glucosidase i variants with improved properties |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015120937A Division JP2015211678A (ja) | 2009-11-20 | 2015-06-16 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013511280A true JP2013511280A (ja) | 2013-04-04 |
| JP2013511280A5 JP2013511280A5 (enExample) | 2014-01-09 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
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| JP2012540120A Pending JP2013511280A (ja) | 2009-11-20 | 2010-11-19 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
| JP2015120937A Withdrawn JP2015211678A (ja) | 2009-11-20 | 2015-06-16 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015120937A Withdrawn JP2015211678A (ja) | 2009-11-20 | 2015-06-16 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9447400B2 (enExample) |
| EP (6) | EP2599862B1 (enExample) |
| JP (2) | JP2013511280A (enExample) |
| CN (2) | CN102844429B (enExample) |
| BR (1) | BR112012011711A2 (enExample) |
| CA (1) | CA2780767A1 (enExample) |
| DK (6) | DK2599863T3 (enExample) |
| IN (1) | IN2012DN03825A (enExample) |
| RU (1) | RU2012125624A (enExample) |
| WO (1) | WO2011063308A2 (enExample) |
Cited By (14)
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| JP2017524363A (ja) * | 2014-08-08 | 2017-08-31 | ザイレコ，インコーポレイテッド | アグリコシル化された酵素およびその使用 |
| JP2019519237A (ja) * | 2016-07-04 | 2019-07-11 | エボニック デグサ ゲーエムベーハーＥｖｏｎｉｋ Ｄｅｇｕｓｓａ ＧｍｂＨ | 変異体ＡｌｋＢ遺伝子 |
| JP2022527235A (ja) * | 2019-01-23 | 2022-06-01 | 義裕 河岡 | インフルエンザウイルスにおける付加的遺伝子に遺伝的安定性を付与する突然変異 |
| US11802273B2 (en) | 2014-06-20 | 2023-10-31 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
| US11851648B2 (en) | 2019-02-08 | 2023-12-26 | Wisconsin Alumni Research Foundation (Warf) | Humanized cell line |
| US12076387B2 (en) | 2010-03-23 | 2024-09-03 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| US12122807B2 (en) | 2019-05-01 | 2024-10-22 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| US12144857B2 (en) | 2018-08-20 | 2024-11-19 | Wisconsin Alumni Research Foundation (Warf) | Vectors for eliciting immune responses to non-dominant epitopes in the hemagglutinin (HA) protein |
| US12290562B2 (en) | 2020-03-25 | 2025-05-06 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
| US12365880B2 (en) | 2020-01-24 | 2025-07-22 | The University Of Tokyo | Recombinant influenza viruses with stabilized NA |
| US12364748B2 (en) | 2016-02-19 | 2025-07-22 | Wisconsin Alumni Research Foundation (Warf) | Influenza B virus replication for vaccine development |
| US12410409B2 (en) | 2011-08-26 | 2025-09-09 | Wisconsin Alumni Research Foundation (Warf) | Influenza viruses with mutant PB2 gene segment as live attenuated vaccines |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102791853B (zh) | 2009-09-23 | 2015-08-12 | 丹尼斯科美国公司 | 新型糖基水解酶及其用途 |
| ES2455269T3 (es) | 2009-11-25 | 2014-04-15 | Codexis, Inc. | Variantes de beta-glucosidasa recombinante para la producción de azúcares solubles de biomasa celulósica |
| WO2011079048A2 (en) | 2009-12-23 | 2011-06-30 | Danisco Us Inc. | Methods for improving the efficiency of simultaneous saccharification and fermentation reactions |
| MX2013002586A (es) | 2010-10-01 | 2013-03-21 | Novozymes Inc | Variantes de beta-glucosidasa y polinucleotidos que codifican las mismas. |
| EP2636738A4 (en) * | 2010-11-05 | 2014-04-30 | Asahi Glass Co Ltd | TRANSFORMANT OF YEAST OF THE GENUS SCHIZOSACCHAROMYCES AND MANUFACTURING METHOD THEREFOR |
| US20140134677A1 (en) | 2011-03-17 | 2014-05-15 | Danisco Us Inc. | Method for reducing viscosity in saccharification process |
| CN102816748B (zh) * | 2011-06-09 | 2014-07-16 | 中国科学院上海生命科学研究院 | 一种新型β-葡萄糖苷酶及其编码基因和应用 |
| US9879245B2 (en) | 2012-12-07 | 2018-01-30 | Danisco Us Inc. | Polypeptides having beta-mannanase activity and methods of use |
| ES2521940B1 (es) | 2013-05-10 | 2015-09-09 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Variantes de beta-glucosidasa con actividad de transglicosilación reducida |
| BR112016002067A2 (pt) | 2013-07-29 | 2017-08-29 | Danisco Us Inc | Variantes de enzimas |
| US10077436B2 (en) | 2013-09-17 | 2018-09-18 | Edeniq, Inc. | Beta-glucosidase enzymes for increased biomass saccharification |
| RU2739004C1 (ru) * | 2014-02-26 | 2020-12-21 | Кхр. Хансен А/С | Варианты химозина с улучшенными молокосвертывающими свойствами |
| US20170362621A1 (en) | 2014-12-18 | 2017-12-21 | Danisco Us Inc. | Engineered multifunctional enzymes and methods of use |
| WO2016100825A1 (en) | 2014-12-18 | 2016-06-23 | Danisco Us Inc | Engineered multifunctional enzymes and methods of use |
| CN107429267B (zh) * | 2014-12-19 | 2021-07-16 | 丹尼斯科美国公司 | 葡糖淀粉酶共混物 |
| WO2017007907A1 (en) | 2015-07-07 | 2017-01-12 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| EP3387140A1 (en) * | 2015-12-10 | 2018-10-17 | DSM IP Assets B.V. | Beta-glucosidase and uses thereof |
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