MX2017001596A - Enzima aglicosilada y usos de la misma. - Google Patents

Enzima aglicosilada y usos de la misma.

Info

Publication number
MX2017001596A
MX2017001596A MX2017001596A MX2017001596A MX2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A
Authority
MX
Mexico
Prior art keywords
cellobiase
enzyme
aglycosylated
present
reesei
Prior art date
Application number
MX2017001596A
Other languages
English (en)
Inventor
Craig Masterman Thomas
Pangilinan Desiree
Yoshida Aiichiro
Landry Sean
Lynch James
KREDER Natasha
Medoff Marshall
Original Assignee
Xyleco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xyleco Inc filed Critical Xyleco Inc
Publication of MX2017001596A publication Critical patent/MX2017001596A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

La presente invencion se relaciona con composiciones que comprenden un polipeptido aglicosilado que tiene actividad de celobiasa y a metodos para la produccion y uso de las mismas. La presente invencion se basa, al menos en parte, en el descubrimiento sorprendente de que una celobiasa de Trichoderma reesei que fue expresada en una linea celular no fungica y aislada a partir de una celula huesped que no glicosila significativamente la enzima, tuvo actividad especifica mas alta en sustrato puro que la celobiasa endogena (glicosilada y secretada) de T. reesei.
MX2017001596A 2014-08-08 2015-08-07 Enzima aglicosilada y usos de la misma. MX2017001596A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462035346P 2014-08-08 2014-08-08
PCT/US2015/044136 WO2016022878A1 (en) 2014-08-08 2015-08-07 Aglycosylated enzyme and uses thereof

Publications (1)

Publication Number Publication Date
MX2017001596A true MX2017001596A (es) 2017-05-15

Family

ID=55264604

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017001596A MX2017001596A (es) 2014-08-08 2015-08-07 Enzima aglicosilada y usos de la misma.

Country Status (16)

Country Link
US (2) US10131894B2 (es)
EP (1) EP3177719A4 (es)
JP (1) JP2017524363A (es)
KR (1) KR20170040246A (es)
CN (1) CN106536730A (es)
AP (1) AP2016009640A0 (es)
AU (1) AU2015300897A1 (es)
BR (1) BR112017002391A2 (es)
CA (1) CA2955960A1 (es)
CU (1) CU20170011A7 (es)
EA (1) EA201790054A1 (es)
IL (1) IL250272A0 (es)
MX (1) MX2017001596A (es)
PH (1) PH12016502604A1 (es)
SG (1) SG11201610894UA (es)
WO (1) WO2016022878A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201790054A1 (ru) 2014-08-08 2017-07-31 Ксилеко, Инк. Агликозилированные ферменты и их применение

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5028515B2 (es) 1971-09-30 1975-09-16
US6103464A (en) * 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
CA2392060C (en) 1999-11-19 2007-07-10 Yissum Research Development Company Of The Hebrew University In Jerusalem Aspergillus niger beta-glucosidase gene, protein and uses thereof
US6946277B2 (en) 2001-01-31 2005-09-20 Council Of Scientific And Industrial Research Method for enhancing cellobiase activity of termitomyces clypeatus using a glycosylation inhibitor
FI120045B (fi) * 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
UA100673C2 (uk) 2006-07-21 2013-01-25 Ксилеко, Инк. Спосіб переробки целюлозної або лігноцелюлозної біомаси (варіанти)
CN101516906B (zh) 2006-07-21 2013-11-06 诺维信股份有限公司 提高具有生物学活性之多肽的分泌的方法
EP2037274A1 (de) 2007-09-11 2009-03-18 GALAB Technologies GmbH Lektinbasierter Glykan-Assay
FR2935986B1 (fr) * 2008-09-12 2012-11-23 Inst Francais Du Petrole Variants de beta-glucosidase a activite amelioree et leurs utilisations.
CA2761305A1 (en) 2009-05-20 2010-11-25 Xyleco, Inc. Processing hydrocarbon-containing materials
DK2602317T3 (da) * 2009-11-20 2017-11-13 Danisco Us Inc Beta-glucosidasevarianter med forbedrede egenskaber
NZ604846A (en) 2010-07-19 2014-11-28 Xyleco Inc Processing biomass
EP2630246A2 (en) 2010-10-20 2013-08-28 Xyleco, Inc. Method for treating lignocellulosic material by irradiating with an electron beam
US20140073017A1 (en) * 2011-03-17 2014-03-13 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
WO2013096698A1 (en) 2011-12-22 2013-06-27 Xyleco, Inc. Processing of biomass materials
FR2994981B1 (fr) 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
US20150307871A1 (en) 2012-12-07 2015-10-29 Novozymes A/S Method for generating site-specific mutations in filamentous fungi
EA201790054A1 (ru) * 2014-08-08 2017-07-31 Ксилеко, Инк. Агликозилированные ферменты и их применение

Also Published As

Publication number Publication date
JP2017524363A (ja) 2017-08-31
CN106536730A (zh) 2017-03-22
EP3177719A4 (en) 2018-04-04
WO2016022878A1 (en) 2016-02-11
AU2015300897A1 (en) 2017-01-05
KR20170040246A (ko) 2017-04-12
CA2955960A1 (en) 2016-02-11
IL250272A0 (en) 2017-03-30
US20170137797A1 (en) 2017-05-18
EA201790054A1 (ru) 2017-07-31
PH12016502604A1 (en) 2017-04-24
CU20170011A7 (es) 2017-08-08
US10131894B2 (en) 2018-11-20
US20190024068A1 (en) 2019-01-24
EP3177719A1 (en) 2017-06-14
AP2016009640A0 (en) 2016-12-31
BR112017002391A2 (pt) 2017-11-28
SG11201610894UA (en) 2017-01-27

Similar Documents

Publication Publication Date Title
MX2020006117A (es) Integracion dirigida de acidos nucleicos.
EP3957711A3 (en) Detergent composition comprising amylase and protease variants
AU2015317711B2 (en) Compositions comprising recombinant Bacillus cells and another biological control agent
EP3708660A3 (en) Polypeptides
MX2017014125A (es) Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
MX2021005358A (es) Metodos y composiciones que comprenden polipeptidos recombinantes purificados.
MX2023001119A (es) Variantes de enzimas y polinucleotidos que las codifican.
IN2014CN02100A (es)
PH12017500785A1 (en) INTERFERON a2B VARIANTS
EP4273238A3 (en) Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
MX2019000140A (es) Variantes de xilanasa y polinucleotidos que las codifican.
WO2018175636A3 (en) Compositions and methods for immunooncology
WO2016022377A3 (en) Methods for modulating the glycosylation profile of recombinant proteins using dissolved oxygen
MY182924A (en) Enzyme compositions and uses thereof
GB2568181A (en) Wheat
WO2014152137A3 (en) Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
MX349517B (es) Polipeptidos que tienen actividad de proteasa y polinucleotidos que codifican los mismos.
PH12018502656A1 (en) Engineered beta-glucosidases and glycosylation methods
EP4067485A3 (en) Lipase variants and polynucleotides encoding same
MX350391B (es) Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican.
MX2022000425A (es) Metodos y composiciones que comprenden un nivel reducido de proteinas de celulas hospedadoras.
WO2015095240A3 (en) Biosynthesis of 1-undecence and related terminal olefins
WO2017173324A3 (en) Alpha-amylases, compositions & methods
MX2015007775A (es) Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican.
MX2016000128A (es) Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.