CU20170011A7 - Composiciones que comprenden un polipéptido aglicosilado y métodos para la producción de las mismas - Google Patents

Composiciones que comprenden un polipéptido aglicosilado y métodos para la producción de las mismas

Info

Publication number
CU20170011A7
CU20170011A7 CUP2017000011A CU20170011A CU20170011A7 CU 20170011 A7 CU20170011 A7 CU 20170011A7 CU P2017000011 A CUP2017000011 A CU P2017000011A CU 20170011 A CU20170011 A CU 20170011A CU 20170011 A7 CU20170011 A7 CU 20170011A7
Authority
CU
Cuba
Prior art keywords
compositions
production
methods
polypeptide
cellobiase
Prior art date
Application number
CUP2017000011A
Other languages
English (en)
Inventor
Natasha Kreder
Sean Landry
James Lynch
Thomas Craig Masterman
Marshall Medoff
Desiree Pangilinan
Aiichiro Yoshida
Original Assignee
Xyleco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xyleco Inc filed Critical Xyleco Inc
Publication of CU20170011A7 publication Critical patent/CU20170011A7/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

<p>La presente invención se relaciona con composiciones que comprenden un polipéptido aglicosilado que tiene actividad de celobiasa y a métodos para la producción y uso de las mismas. La presente invención se basa, al menos en parte, en el descubrimiento sorprendente de que una celobiasa de Trichoderma reesei que fue expresada en una línea celular no fúngica y aislada a partir de una célula huésped que no glicosila significativamente la enzima, tuvo actividad específica más alta en sustrato puro que la celobiasa endógena (glicosilada y secretada) de T. reesei.</p>
CUP2017000011A 2014-08-08 2015-08-07 Composiciones que comprenden un polipéptido aglicosilado y métodos para la producción de las mismas CU20170011A7 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462035346P 2014-08-08 2014-08-08
PCT/US2015/044136 WO2016022878A1 (en) 2014-08-08 2015-08-07 Aglycosylated enzyme and uses thereof

Publications (1)

Publication Number Publication Date
CU20170011A7 true CU20170011A7 (es) 2017-08-08

Family

ID=55264604

Family Applications (1)

Application Number Title Priority Date Filing Date
CUP2017000011A CU20170011A7 (es) 2014-08-08 2015-08-07 Composiciones que comprenden un polipéptido aglicosilado y métodos para la producción de las mismas

Country Status (16)

Country Link
US (2) US10131894B2 (es)
EP (1) EP3177719A4 (es)
JP (1) JP2017524363A (es)
KR (1) KR20170040246A (es)
CN (1) CN106536730A (es)
AP (1) AP2016009640A0 (es)
AU (1) AU2015300897A1 (es)
BR (1) BR112017002391A2 (es)
CA (1) CA2955960A1 (es)
CU (1) CU20170011A7 (es)
EA (1) EA201790054A1 (es)
IL (1) IL250272A0 (es)
MX (1) MX2017001596A (es)
PH (1) PH12016502604A1 (es)
SG (1) SG11201610894UA (es)
WO (1) WO2016022878A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201790054A1 (ru) 2014-08-08 2017-07-31 Ксилеко, Инк. Агликозилированные ферменты и их применение

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5028515B2 (es) 1971-09-30 1975-09-16
US6103464A (en) * 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
CA2392060C (en) 1999-11-19 2007-07-10 Yissum Research Development Company Of The Hebrew University In Jerusalem Aspergillus niger beta-glucosidase gene, protein and uses thereof
US6946277B2 (en) 2001-01-31 2005-09-20 Council Of Scientific And Industrial Research Method for enhancing cellobiase activity of termitomyces clypeatus using a glycosylation inhibitor
FI120045B (fi) * 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
UA100673C2 (uk) 2006-07-21 2013-01-25 Ксилеко, Инк. Спосіб переробки целюлозної або лігноцелюлозної біомаси (варіанти)
CN101516906B (zh) 2006-07-21 2013-11-06 诺维信股份有限公司 提高具有生物学活性之多肽的分泌的方法
EP2037274A1 (de) 2007-09-11 2009-03-18 GALAB Technologies GmbH Lektinbasierter Glykan-Assay
FR2935986B1 (fr) * 2008-09-12 2012-11-23 Inst Francais Du Petrole Variants de beta-glucosidase a activite amelioree et leurs utilisations.
CA2761305A1 (en) 2009-05-20 2010-11-25 Xyleco, Inc. Processing hydrocarbon-containing materials
DK2602317T3 (da) * 2009-11-20 2017-11-13 Danisco Us Inc Beta-glucosidasevarianter med forbedrede egenskaber
NZ604846A (en) 2010-07-19 2014-11-28 Xyleco Inc Processing biomass
EP2630246A2 (en) 2010-10-20 2013-08-28 Xyleco, Inc. Method for treating lignocellulosic material by irradiating with an electron beam
US20140073017A1 (en) * 2011-03-17 2014-03-13 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
WO2013096698A1 (en) 2011-12-22 2013-06-27 Xyleco, Inc. Processing of biomass materials
FR2994981B1 (fr) 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
US20150307871A1 (en) 2012-12-07 2015-10-29 Novozymes A/S Method for generating site-specific mutations in filamentous fungi
EA201790054A1 (ru) * 2014-08-08 2017-07-31 Ксилеко, Инк. Агликозилированные ферменты и их применение

Also Published As

Publication number Publication date
JP2017524363A (ja) 2017-08-31
MX2017001596A (es) 2017-05-15
CN106536730A (zh) 2017-03-22
EP3177719A4 (en) 2018-04-04
WO2016022878A1 (en) 2016-02-11
AU2015300897A1 (en) 2017-01-05
KR20170040246A (ko) 2017-04-12
CA2955960A1 (en) 2016-02-11
IL250272A0 (en) 2017-03-30
US20170137797A1 (en) 2017-05-18
EA201790054A1 (ru) 2017-07-31
PH12016502604A1 (en) 2017-04-24
US10131894B2 (en) 2018-11-20
US20190024068A1 (en) 2019-01-24
EP3177719A1 (en) 2017-06-14
AP2016009640A0 (en) 2016-12-31
BR112017002391A2 (pt) 2017-11-28
SG11201610894UA (en) 2017-01-27

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