CN105309083B - A kind of Paeonia papaveracea seed accelerating germination method - Google Patents
A kind of Paeonia papaveracea seed accelerating germination method Download PDFInfo
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- CN105309083B CN105309083B CN201510747989.7A CN201510747989A CN105309083B CN 105309083 B CN105309083 B CN 105309083B CN 201510747989 A CN201510747989 A CN 201510747989A CN 105309083 B CN105309083 B CN 105309083B
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Abstract
The invention discloses a kind of Paeonia papaveracea seed accelerating germination method, including releasing Paeonia papaveracea seed top-wall effect and hypocotyl dormancy method, the releasing Paeonia papaveracea seed hypocotyl dormancy method, including by sterilized Paeonia papaveracea seed with concentration be 100mg/L to 700mg/LGA3Immersion, matrix Stratificated treatment.The releasing Paeonia papaveracea seed top-wall effect method, including vernalization processing is carried out respectively from 0.5cm≤root long ＜ 3cm and root length >=3cm seed.It is 100mg/L to 400mg/LGA with concentration that the Paeonia papaveracea seed of root, which will have been urged,3Immersion, matrix Stratificated treatment is placed in vernalization, moisturizing under 25 DEG C of constant temperature.The present invention shortens germinating time using the dual dormant technology for releasing Paeonia papaveracea seed, is only needed 97 days or so from germination is seeded into, emergence rate reaches as high as more than 90%, meets and suitable for large-scale promotion cultivation.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of Paeonia papaveracea seed accelerating germination method.Including releasing
Paeonia papaveracea seed top-wall effect and hypocotyl dormancy method.
Background technology
Tree peony, particularly Paeonia papaveracea, are also important oil Varieties of Peony not only with very high sight
One of, seeding and seedling raising is the important channel for obtaining big gauging tree peony seedling.But it is unprocessed or only soaked through warm water
The Paeonia papaveracea seed planted is seeded in behind crop field, and germination percentage is very low, less than 30%, and the sprouting phase of taking root needs more than half a year, sternly
The cultivation for constraining Paeonia papaveracea again is promoted.It is that Paeonia suffruticosa seed has dual hibernation feature to trace it to its cause, and is in particular in radicle
(hypocotyl) and the dormancy of plumule (epicotyl), it is to effectively improve the quick neat seedling of Paeonia papaveracea to release the red dual dormancy of seed of phoenix
And the key point that commerial growing is promoted.A kind of entitled " the quickly breeding Feng Dan of number of patent application 2014101493970
The patent of invention of the breeding method of seedling ", manually breaks the red seed top-wall effect of phoenix, its technical scheme is to take root in advance
Seed after the long sand storage more than 4cm, is 200~400mg/L Gibberellins solutions with its concentration, soaks 20~28 hours, carry out
Break top-wall effect processing.Number of patent application 2012105204538 is entitled " rapidly and efficiently to break embryo on Paeonia suffruticosa seed
The patent of invention of the method for axle dormancy ", its technical scheme is to choose full Paeonia suffruticosa seed, use GA3（Gibberellin）1000mg/L
+T20（Tween）100uL/L treatment fluids I soak 24h, kind of a shell are divested, with GA3100mg/L+BA1mg/L+Ca (NO3) 25mg/L+
T2（Tween）10uL/L treatment fluids II soak 24h.In the prior art, also have and seed is soaked using Gibberellins solution or chemicals for breaking dormancy
Processing, to release the red seed top-wall effect of phoenix.Although above method can also improve the red percentage of seedgermination of phoenix to a certain extent,
But effect is not ideal enough, cost is cultivated higher.
The content of the invention
It is an object of the invention to overcome deficiency of the prior art there is provided " a kind of Paeonia papaveracea seed accelerating germination method ",
The present invention is greatly improved Paeonia papaveracea emergence rate using the dual dormant technology for releasing Paeonia papaveracea seed, when shortening germination
Between, reduce cost.This method is simple and practical, and with low cost, management is convenient, has not only urged root but also vernalization, and 97 are only needed from germination is seeded into
It or so, emergence rate reaches as high as more than 90%, meets and suitable for large-scale promotion cultivation.
To achieve these goals, the present invention is achieved through the following technical solutions：A kind of Paeonia papaveracea seed is urged
Bud method, main technical scheme is, including releases Paeonia papaveracea seed top-wall effect and hypocotyl dormancy method, the solution
Except Paeonia papaveracea seed hypocotyl dormancy method, comprise the following steps：
1）Seed treatment, soaks sterilization in 20 ~ 30 minutes, and use clear water with concentration for 0.1% ~ 0.3% liquor potassic permanganate
Clean and dry in the shade standby.
2）With concentration it is 100mg/L to 700mg/LGA by sterilized Paeonia papaveracea seed3Immersion 48 hours.
3）Root, moisturizing are urged under matrix Stratificated treatment, the constant temperature for being placed in 25 DEG C.
The releasing Paeonia papaveracea seed top-wall effect method, comprises the following steps：
1）Seed selection, vernalization processing is carried out from 0.5cm≤root long ＜ 3cm and root length >=3cm seed respectively.
2）It is 100mg/L to 400mg/LGA with concentration that the Paeonia papaveracea seed of root, which will have been urged,3Immersion 1 to 2 hour.
3）Matrix Stratificated treatment, is placed in vernalization, moisturizing under 25 DEG C of constant temperature.
Further technical scheme is, step 3）Described in matrix lamination be processed as sand lamination, turf lamination or coconut palm
One kind in chaff layer product.
Further technical scheme is that the matrix Stratificated treatment comprises the following steps：
1）Matrix is sterilized, and matrix is placed at 150 DEG C of high temperature into continuous baking 2 hours, cooling is taken out standby.
2）Stratificated treatment, bottom matrix 5mm is thick, and soaked Paeonia papaveracea seed is evenly distributed in bottom matrix,
In Paeonia papaveracea seed overlying lid overlayer matrix.
3）Water, moisturizing, the moisturizing with matrix not only fully absorb water but also be advisable without ponding.
The invention has the advantages that：
1st, the present invention is greatly improved Paeonia papaveracea emergence rate using the dual dormant technology for releasing Paeonia papaveracea seed,
Shorten germinating time, reduce cost.This method is simple and practical, and with low cost, management is convenient, has not only urged root but also vernalization, from being seeded into
Germination is only needed 97 days or so, and emergence rate reaches as high as more than 90%, is met and suitable for large-scale promotion cultivation.
Brief description of the drawings
Fig. 1 is influence variance analysis of the different disposal to the releasing hypocotyl dormancy of Paeonia papaveracea seed in the embodiment of the present invention
Table.Note：As a result it is 3 repetition average values, it is notable in P=0.05 level difference with the numeral of different alphabetic flags, with a, b,
C ... represents the difference between different gibberellin concentration, similarly hereinafter.
Fig. 2 is the influence analysis of variance table that different disposal releases top-wall effect to Paeonia papaveracea in the embodiment of the present invention.
Embodiment
Below by non-limiting example, the present invention is expanded on further, the present invention is understood.
Embodiment 1
As shown in figure 1, a kind of release Paeonia papaveracea seed hypocotyl dormancy method, comprise the following steps：
The releasing Paeonia papaveracea seed hypocotyl dormancy method, comprises the following steps：
1）Seed treatment, sterilization in 30 minutes is soaked with concentration for 0.3% liquor potassic permanganate, and dry with distilled water flushing
Only dry in the shade standby；With cold water configure liquor potassic permanganate, with.
2）By it is sterilized and with the clean Paeonia papaveracea seed of distilled water flushing respectively by 100mg/L gibberellin（GA3）,
300mg/LGA3, 500mg/LGA3, 700mg/LGA3Soak 48h.Distilled water immersion is control（CK）.
3）Then different substrates are taken the seed after immersion treatment to carry out Stratificated treatment, specially sand lamination respectively,
Turf lamination, coconut palm chaff lamination.100 seeds of every kind of processing, 3 repetitions.
4）Stratificated treatment concrete operations are that matrix is placed at 150 DEG C of high temperature continuous baking 2 hours, taken out cold by matrix sterilizing
It is standby.Bottom matrix 5mm is thick, soaked Paeonia papaveracea seed is evenly distributed in bottom matrix, in Paeonia papaveracea kind
Sub- overlying lid overlayer matrix.Water, moisturizing, the moisturizing with matrix not only fully absorb water but also be advisable without ponding.The perseverance of 25 DEG C of placement
In warm condition, observe on time daily, originating number of days, root of hair to root of hair goes through phase, Rooting percent, root length etc. and recorded and counted.
Seed root of hair：Kind of a skin 0.5cm is stretched out with radicle and is considered as seed root of hair.
Root of hair originates number of days：From matrix lamination to the 1st day required for seed root of hair.
Root of hair goes through the phase：I.e. from last 1 seed root of hair elapsed time during the 1st seed root of hair to statistics, root of hair is high
It is then to terminate that continuous 15d occurs there is no root of hair seed behind peak.
Embodiment 2
As shown in Fig. 2 a kind of release Paeonia papaveracea seed top-wall effect method, comprise the following steps：
1）Seed selection, vernalization processing is carried out from 0.5cm≤root long ＜ 3cm and root length >=3cm seed respectively.
2）It is 100mg/L, 200 mg/L, 300 mg/L and 400 mg/L with concentration that the Paeonia papaveracea seed of root, which will have been urged,
Gibberellin soaks 2h or 1h.
3）Matrix Stratificated treatment, is placed in vernalization, moisturizing under 25 DEG C of constant temperature.Each processing is divided into 3 parts and carried out respectively
Sand lamination, turf lamination, coconut palm chaff Stratificated treatment is placed under 25 DEG C of constant temperatures, notes moisturizing.
Stratificated treatment, method for moisturizing are same as embodiment 1.
As shown in figure 1, by implementation and application to Paeonia papaveracea seed accelerating germination method of the present invention, as a result showing to release purple
In spot Paeonia suffruticosa seed hypocotyl dormancy method embodiment, with 500mg/LGA3Soak coconut palm chaff lamination under 48h and 25 DEG C of constant temperatures
Processing Paeonia papaveracea seed carries out urging root treatment effect optimal, with 500mg/LGA3Soak turf under 48h and 25 DEG C of constant temperatures
Stratificated treatment Paeonia papaveracea seed carries out urging root processing to take second place.
As shown in Fig. 2 releasing in Paeonia papaveracea seed top-wall effect embodiment of the method, grown up with Paeonia papaveracea seminal root
In being soaked after 3cm with 300mg/L gibberellin after 1h, wet sand lamination carries out vernalization treatment effect most preferably under 25 DEG C of constant temperatures, with purple
Spot Paeonia suffruticosa seed root length is more than after 3cm carries out vernalization with turf lamination under 25 DEG C of constant temperatures after 300mg/L gibberellin immersion 1h
Processing is taken second place.
In summary, the present invention is greatly improved Paeonia papaveracea using the dual dormant technology for releasing Paeonia papaveracea seed
Emergence rate, shortens germinating time, reduces cost.This method is simple and practical, and with low cost, management is convenient, has not only urged root but also vernalization, from
Being seeded into germination only needs 97 days or so, and emergence rate reaches as high as more than 90%, meets and suitable for large-scale promotion cultivation.
Claims (2)
1. a kind of Paeonia papaveracea seed accelerating germination method, including release Paeonia papaveracea seed top-wall effect and hypocotyl dormancy side
Method, it is characterised in that the releasing Paeonia papaveracea seed hypocotyl dormancy method, comprises the following steps：
1）Seed treatment, soaks sterilization in 30 minutes, and dry in the shade standby with clear water is clean with concentration for 0.3% liquor potassic permanganate
With；
2）By sterilized Paeonia papaveracea seed with concentration be 500mg/L GA3 soak 48 hours；
3）Root, moisturizing are urged under coconut palm chaff matrix Stratificated treatment, the constant temperature for being placed in 25 DEG C；
The releasing Paeonia papaveracea seed top-wall effect method, comprises the following steps：
1）Seed selection, the seed for being more than 3cm from root length carries out vernalization processing；
2）It is that 300mg/LGA3 soaks 1 hour with concentration that the Paeonia papaveracea seed of root, which will have been urged,；
3）Sand matrix Stratificated treatment, is placed in vernalization, moisturizing under 25 DEG C of constant temperature.
2. a kind of Paeonia papaveracea seed accelerating germination method according to claim 1, it is characterised in that at the matrix lamination
Reason, comprises the following steps：
1）Matrix is sterilized, and matrix is placed at 150 DEG C of high temperature into continuous baking 2 hours, cooling is taken out standby；
2）Stratificated treatment, bottom matrix 5mm is thick, soaked Paeonia papaveracea seed is evenly distributed in bottom matrix, in purple
Spot Paeonia suffruticosa seed overlying lid overlayer matrix；
3）Water, moisturizing, the moisturizing with matrix not only fully absorb water but also be advisable without ponding.
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| CN105766129A (en) * | 2016-03-11 | 2016-07-20 | 西北农林科技大学 | Method for releasing dormancy of oil-used peony seeds |
| CN111149592A (en) * | 2018-11-08 | 2020-05-15 | 甘肃盛世牡丹生物科技有限公司 | Breeding method of paeonia rockii |
| CN109452127B (en) * | 2019-01-07 | 2021-07-13 | 云南省农业科学院热带亚热带经济作物研究所 | Seedling raising substrate for improving emergence rate of fructus aurantii and application thereof |
| CN109984007A (en) * | 2019-04-22 | 2019-07-09 | 陇南师范高等专科学校 | A method of it improving wild Paeonia papaveracea seed and takes root |
| CN110073887A (en) * | 2019-06-05 | 2019-08-02 | 陇南师范高等专科学校 | A method of improving Paeonia papaveracea seeding ratio |
| CN114631459B (en) * | 2022-04-07 | 2023-07-07 | 云南省农业科学院高山经济植物研究所 | Method for rapid seedling formation of Yunnan peony seeds |
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| CN103907424A (en) * | 2014-04-15 | 2014-07-09 | 铜陵创新农业科技有限公司 | Cultivation method for rapidly cultivating paeonia ostii seedlings |
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