CN104770100B - A kind of method releasing Paeonia ostii seed top-wall effect - Google Patents
A kind of method releasing Paeonia ostii seed top-wall effect Download PDFInfo
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Abstract
The invention discloses a kind of method releasing Paeonia ostii seed top-wall effect, comprise the steps: 1) seed selection, 2) seed treatment, 3) root is urged, 4) when the Paeonia ostii seed base-root that major part has urged root grows to 2-4cm, choose the seed that root length is 3cm and be placed under 1-5 DEG C of environment and carry out K cryogenic treatment 60 days；The seed of K cryogenic treatment was sowed in late December；The Paeonia ostii seed K cryogenic treatment sowed again is placed in 20d in 16-20 DEG C of greenhouse and carries out Routine Management.The present invention is simple and practical, with low cost, convenient management, is urging on pile foundation, K cryogenic treatment 60d can efficient solution except Paeonia ostii top-wall effect, its germination percentage up to 87.9%, the long 6.2cm of bud, meet and be suitable to large-scale promotion cultivation.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of method releasing Paeonia ostii seed top-wall effect.
Background technology
Paeonia suffruticosa, particularly Paeonia ostii Paeonia suffruticosa, be the oily dual-purpose improved seeds of medicine.Oil becomes research with the research and development of Paeonia suffruticosa
New focus.Seeding and seedling raising is the important channel obtaining big gauging Paeonia suffruticosa Seedling, but, seed of Paeonia suffruticosa germination one under natural conditions
As last 8～12 months, and emergence rate is relatively low, seriously constrains the oil cultivation of Paeonia suffruticosa and promotes.Tracing it to its cause is Paeonia suffruticosa kind
Son has dual hibernation feature, is in particular in radicle (hypocotyl) and the dormancy of plumule (epicotyl), especially with top-wall effect
The deepest.After seed autumn sowing, radicle growth become root and epicotyl in a dormant state, it is necessary to after low temperature after a while
Epicotyl could be sprouted, and constrains oil Paeonia suffruticosa quickly neat Seedling and commerial growing and promotes.Release Paeonia ostii seed top-wall effect
It is to be effectively improved the oil Paeonia suffruticosa key point that quickly neat Seedling and commerial growing are promoted.Number of patent application 2014101493970
The patent of invention of invention entitled " breeding method of a kind of quickly breeding Paeonia ostii seedling ", breaks on Paeonia ostii seed the most in advance
Plumular axis dormancy, its technical scheme is, takes the root length seed more than the Sha Zanghou of 4cm, is 200～400mg/L red mould by its concentration
Cellulose solution, soaks 20～28 hours, carries out breaking top-wall effect and processes.Number of patent application 2012105204538 denomination of invention
For the patent of invention of " method rapidly and efficiently breaking seed of Paeonia suffruticosa top-wall effect ", its technical scheme is, chooses full male
Red seed, uses GA3(gibberellins) 1000mg/L+T20(tween) 100uL/L treatment fluid I soaks 24h, and divest in step and obtain
The kind shell of seed of Paeonia suffruticosa, with GA3100mg/L+BA1mg/L+Ca (NO3) 25mg/L+T2(tween) 10uL/L treatment fluid II soaks
Bubble 24h.In prior art, also there are employing Gibberellins solution or chemicals for breaking dormancy to seed immersion treatment, to release embryo on Paeonia ostii seed
Axle dormancy.Although above method the most also can improve Paeonia ostii percentage of seedgermination, but effect is not ideal enough, cultivates cost
Higher.
Summary of the invention
It is an object of the invention to overcome deficiency of the prior art, it is provided that " a kind of releasing Paeonia ostii seed top-wall effect
Method ", the present invention abundant Adjust and use Paeonia ostii seed body naturally contained ABA(growth inhibitor, i.e. abscisic acid) and GA3
The content of (growth promoter, i.e. gibberellins), reaches to release the purpose of Paeonia ostii seed top-wall effect, effectively shortens and improve phoenix
Red percentage of seedgermination, the method is simple and practical, with low cost, convenient management, is urging on pile foundation, and K cryogenic treatment 60 days can be effective
Release Paeonia ostii top-wall effect, its germination percentage up to 87.9%, the long 6.2cm of bud, meet and be suitable to large-scale promotion cultivation.
To achieve these goals, the present invention is achieved through the following technical solutions: on a kind of releasing Paeonia ostii seed
The method of plumular axis dormancy, comprises the steps:
1) seed selection, first chooses the fruit picking up from maternal plant at the beginning of 8 months, within 10-15 days disposed within, completes physiology in shell
The seed of after-ripening；
2) seed treatment, before sowing, after being rinsed with clear water by seed, takes off heavy full seed, is 1g/L's by concentration
It is standby that potassium permanganate solution soaks 1h sterilization；
3) urge root, before sowing, the most sterilized Paeonia ostii seed and wet sand are pressed the mixing of 1:4 volume ratio, is placed in 21-25 DEG C
In greenhouse, lamination urges root 40 days standby；Described wet sand be humidity be the sand of sand maximum water-holding capacity 50%；
4) low temperature releases top-wall effect.
Further technical scheme, low temperature described in step 4) releases top-wall effect, comprises the steps:
1) when the Paeonia ostii seed base-root that major part has urged root grows to 2-4cm, choose the seed that root length is 3cm and be placed in
K cryogenic treatment is carried out 60 days under 1-5 DEG C of environment；
2) seed of K cryogenic treatment was sowed in late December.
Preferably, described low temperature releases top-wall effect, comprises the steps:
1) when major part Paeonia ostii seed base-root grows to 2-4cm, choose the seed that root length is 3cm and be placed under 5 DEG C of environment
Row K cryogenic treatment 60 days；
2) 5 DEG C of seeds processed were sowed in late December.
Preferably, described low temperature releases top-wall effect, comprises the steps:
1) when major part Paeonia ostii seed base-root grows to 2-4cm, choose the seed that root length is 3cm and be placed under 3 DEG C of environment
Row K cryogenic treatment 60 days；
2) 3 DEG C of seeds processed were sowed in late December.
It is furthermore preferred that described low temperature releases top-wall effect, comprise the steps:
1) when major part Paeonia ostii seed base-root grows to 2-4cm, choose the seed that root length is 3cm and be placed under 1 DEG C of environment
Row K cryogenic treatment 60 days；
2) 1 DEG C of seed processed was sowed in late December.
Further technical scheme is, the Paeonia ostii seed after K cryogenic treatment being terminated is placed in 16-20 DEG C of greenhouse 20 days
Carry out Routine Management.
Present invention have the advantage that
1, the present invention abundant Adjust and use Paeonia ostii seed body naturally contained ABA(growth inhibitor, i.e. abscisic acid) and GA3
The content of (growth promoter, i.e. gibberellins), reaches to release the purpose of Paeonia ostii seed top-wall effect, effectively shortens and improve phoenix
Red percentage of seedgermination, the method is simple and practical, with low cost, convenient management, is urging on pile foundation, and K cryogenic treatment 60 days can be effective
Release Paeonia ostii top-wall effect, its germination percentage up to 87.9%, the long 6.2cm of bud, meet and be suitable to large-scale promotion cultivation.
Accompanying drawing explanation
Fig. 1 is that treatment of different temperature of the present invention is to Paeonia ostii seed treatment GA3(gibberellins) changes of contents curve synoptic diagram.
Fig. 2 is that treatment of different temperature of the present invention is to Paeonia ostii seed treatment ABA(abscisic acid) changes of contents curve synoptic diagram.
Fig. 3 is that treatment of different temperature of the present invention is to Paeonia ostii seed treatment GA3/ABA content ratio change curve schematic diagram.
Detailed description of the invention
Below by non-limiting example, the present invention is expanded on further, understands the present invention.
Embodiment 1
A kind of method releasing Paeonia ostii seed top-wall effect, comprises the steps:
1) seed selection, first chooses the fruit picking up from maternal plant at the beginning of 8 months, within 10-15 days disposed within, completes physiology in shell
The seed of after-ripening；
2) seed treatment, before sowing, after being rinsed with clear water by seed, takes off heavy full seed, is 1g/L's by concentration
It is standby that potassium permanganate solution soaks 1h sterilization；
3) urge root, before sowing, the most sterilized Paeonia ostii seed and wet sand are pressed the mixing of 1:4 volume ratio, is placed in 21-25 DEG C
In greenhouse, lamination urges root 40 days standby；Described wet sand be humidity be the sand of sand maximum water-holding capacity 50%；
4) when the Paeonia ostii seed base-root that major part has urged root grows to 2-4cm, choose the seed that root length is 3cm and be placed in 1
DEG C, carry out K cryogenic treatment 60 days under 3 DEG C and 5 DEG C of environment；The seed of K cryogenic treatment was sowed in late December；
The Paeonia ostii seed K cryogenic treatment sowed again is placed in 16-20 DEG C of greenhouse 20 days and carries out Routine Management.
Embodiment 2
A kind of method releasing Paeonia ostii seed top-wall effect, comprises the steps:
1) seed selection, first chooses the fruit picking up from maternal plant at the beginning of 8 months, within 10-15 days disposed within, completes physiology in shell
The seed of after-ripening；
2) seed treatment, before sowing, after being rinsed with clear water by seed, takes off heavy full seed, is 1g/L's by concentration
It is standby that potassium permanganate solution soaks 1h sterilization；
3) urge root, before sowing, the most sterilized Paeonia ostii seed and wet sand are pressed the mixing of 1:4 volume ratio, is placed in 21-25 DEG C
In greenhouse, lamination urges root 40 days standby；Described wet sand be humidity be the sand of sand maximum water-holding capacity 50%；
4) when the Paeonia ostii seed base-root that major part has urged root grows to 2-4cm, choose the seed that root length is 3cm and be placed in
K cryogenic treatment is carried out 60 days, observation germination percentage (%) under 10 DEG C, 15 DEG C and 20 DEG C of environment.
As it is shown in figure 1, GA3 is to regulate seed dormant promotion thing, compare treatment of different temperature to Paeonia ostii seed GA3 content
The impact of change, result shows: along with the prolongation of the time of process, and 1 DEG C and 5 DEG C two processes GA3 content in Paeonia ostii seed is elder generation
Downward trend after rising, 1 DEG C of process reaches maximum at 40d, and 5 DEG C of process reach maximum at 50d, the most gradually under
Fall.10 DEG C process in ascendant trend always, but ascensional range is less than 1 DEG C and 5 DEG C two process.15 DEG C and 20 DEG C two process kind
In son, GA3 content maintains reduced levels always.As can be seen here, in 1～10 DEG C of temperature range, process can increase Paeonia ostii seed
Interior GA3 content, thus provide condition for breaking seed top-wall effect, especially with GA3 in process seed in 1～5 DEG C of temperature range
Content increase becomes apparent from, thus is more beneficial for the germination of Paeonia ostii seed.
As in figure 2 it is shown, ABA is to regulate seed dormant mortifier, compare treatment of different temperature to Paeonia ostii seed ABA content
The impact of change, result shows: along with the prolongation of the time of process, except 15 DEG C and 20 DEG C process ABA content are always maintained at relatively Gao Shui
Outside Ping, in other three process Paeonia ostii seeds, ABA content downward trend is obvious, and wherein 1 DEG C and 5 DEG C process fall is all higher than
10 DEG C of process, process fall with 1 DEG C maximum especially.1 DEG C and 5 DEG C processes respectively 30 days and 40 days fall maximums, it
After the most slowly decline.As can be seen here, ABA content in process all can reduce Paeonia ostii seed in 1～10 DEG C of temperature range, especially with 1～5
Process ABA content in seed in DEG C temperature range to decline and become apparent from, be more beneficial for releasing seed top-wall effect, promote seed
Germinate.
As it is shown on figure 3, compare the impact that Paeonia ostii seed GA3/ABA ratio is changed by treatment of different temperature, result shows: with
The ratio of the prolongation of the time of process, GA3 content and ABA content, 15 DEG C and 20 DEG C two process without significant change, compare always
Low.1 DEG C, 5 DEG C and 10 DEG C three process GA3/ABA ratios increased before 20 days slowly, increase sharply afterwards, especially with after 50 days
Amplification is maximum.Between treatment of different temperature, GA3/ABA ratio shows as 5 DEG C of ＞ of 3 DEG C of ＞ of 1 DEG C of ＞, 15 DEG C of ＞ of 10 DEG C of ＞ 20 DEG C, 1 DEG C and
5 DEG C apparently higher than 10 DEG C of process.As can be seen here, in 1～5 DEG C of temperature range, process can increase GA3/ABA ratio in Paeonia ostii seed
Value, regulation Paeonia ostii seed top-wall effect promotes the balance between thing and mortifier.
By the present invention releases enforcement and the application of the method for Paeonia ostii seed top-wall effect, result is as shown in the table:
The impact on Paeonia ostii germination of the table treatment of different temperature
| Temperature (DEG C) | Germination percentage (%) | Bud length (cm) |
| 1 | 87.9 | 6.2 |
| 3 | 83.2 | 5.6 |
| 5 | 80.1 | 5.4 |
| 10 | 41.4 | 0.9 |
| 15 | 0 | 0 |
| 20 | 0 | 0 |
In sum, the naturally contained ABA(growth inhibitor of the present invention abundant Adjust and use Paeonia ostii seed body, i.e. come off
Acid) and GA3(growth promoter, i.e. gibberellins) content, reach releasing Paeonia ostii seed top-wall effect purpose, effectively shorten
And improving Paeonia ostii percentage of seedgermination, the method is simple and practical, with low cost, convenient management, is urging on pile foundation, K cryogenic treatment
60d can efficient solution except Paeonia ostii top-wall effect, its germination percentage up to 87.9%, the long 6.2cm of bud, meet and be suitable to large-scale promotion
Cultivation.
Claims (2)
1. the method releasing Paeonia ostii seed top-wall effect, it is characterised in that comprise the steps:
1) seed selection, first chooses the fruit picking up from maternal plant at the beginning of 8 months, within 10-15 days disposed within, completes physiology after-ripening in shell
Seed；
2) seed treatment, before sowing, after being rinsed with clear water by seed, takes off heavy full seed, is the Gao Meng of 1g/L by concentration
It is standby that acid potassium solution soaks 1h sterilization；
3) urge root, before sowing, the most sterilized Paeonia ostii seed and wet sand are pressed the mixing of 1:4 volume ratio, is placed in 21-25 DEG C of greenhouse
Interior lamination urges root 40 days standby；Described wet sand be humidity be the sand of sand maximum water-holding capacity 50%；
4) low temperature releases top-wall effect, and described low temperature releases top-wall effect, is to treat that major part Paeonia ostii seed base-root grows to 2-
During 4cm, choose the seed that root length is 3cm and be placed under 1 DEG C of environment and carry out K cryogenic treatment 60 days, the seed that 1 DEG C processes is existed
Late December is sowed.
A kind of method releasing Paeonia ostii seed top-wall effect the most according to claim 1, it is characterised in that by
K cryogenic treatment Paeonia ostii seed to be sowed is placed in 16-20 DEG C of greenhouse 20 days and carries out Routine Management.
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| CN105766129A (en) * | 2016-03-11 | 2016-07-20 | 西北农林科技大学 | Method for releasing dormancy of oil-used peony seeds |
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| CN101288360A (en) * | 2008-06-05 | 2008-10-22 | 中国科学院植物研究所 | Method for promoting germination of Paeonia ludlowii seed |
| CN101836528A (en) * | 2010-05-14 | 2010-09-22 | 铜陵凤丹种植科技开发有限责任公司 | Method for improving sprouting rate of peony seeds and promoting root growth of sprouts |
| CN102124839A (en) * | 2010-12-02 | 2011-07-20 | 中国农业科学院蔬菜花卉研究所 | Peony seed culturing container and method for culturing seedling quickly by using same |
| JP2011142903A (en) * | 2009-12-15 | 2011-07-28 | Kinki Univ | Method for raising seedlings of peony |
| CN103843548A (en) * | 2012-12-06 | 2014-06-11 | 上海市上海中学 | Method used for quickly breaking dormancy of peony seed epicotyl |
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2015
- 2015-03-25 CN CN201510132437.5A patent/CN104770100B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288360A (en) * | 2008-06-05 | 2008-10-22 | 中国科学院植物研究所 | Method for promoting germination of Paeonia ludlowii seed |
| JP2011142903A (en) * | 2009-12-15 | 2011-07-28 | Kinki Univ | Method for raising seedlings of peony |
| CN101836528A (en) * | 2010-05-14 | 2010-09-22 | 铜陵凤丹种植科技开发有限责任公司 | Method for improving sprouting rate of peony seeds and promoting root growth of sprouts |
| CN102124839A (en) * | 2010-12-02 | 2011-07-20 | 中国农业科学院蔬菜花卉研究所 | Peony seed culturing container and method for culturing seedling quickly by using same |
| CN103843548A (en) * | 2012-12-06 | 2014-06-11 | 上海市上海中学 | Method used for quickly breaking dormancy of peony seed epicotyl |
Non-Patent Citations (1)
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