CN105284907A - Nematode killing granule containing avermectin and trichoderma harzianum - Google Patents
Nematode killing granule containing avermectin and trichoderma harzianum Download PDFInfo
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- CN105284907A CN105284907A CN201510810050.0A CN201510810050A CN105284907A CN 105284907 A CN105284907 A CN 105284907A CN 201510810050 A CN201510810050 A CN 201510810050A CN 105284907 A CN105284907 A CN 105284907A
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- avermectin
- trichoderma harzianum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention relates to a nematode killing granule containing avermectin and trichoderma harzianum. The active component of the granule is composed of avermectin and trichoderma harzianum. Trichoderma harzianum technical concentrate with a content of 30 billion cfu/g is taken as the 100% technical concentrate. The weight ratio of avermectin to trichoderma harzianum is 2-15:1-5, the content of avermectin in the composition is 0.2 to 1.5%, and the content of trichoderma harzianum in the composition is 0.03-0.3 billion cfu/g. The granule has a synergetic effect, can effectively prevent and treat nematode, is capable of also improving the micro-environment of soil, can make plant root grow more healthily, and well treats soil-borne diseases such as root rot, southern blight, damping off, rice blight, and the like. The avermectin and trichoderma harzianum can be co-used in a reasonable range, the application amounts of single components are both reduced, the drug resistance of avermectin to the nematode is effectively retarded, the usage period of pesticide is prolonged, moreover, the cost is reduced, and the environmental pollution is reduced.
Description
Technical field
The present invention relates to technical field of pesticide, specifically a kind of granular formulation for killing nematode containing Avermectin and trichoderma harzianum.
Background technology
Root-knot nematode and Cyst nematode, be the two class nematodes the heaviest to the harm of agricultural production, the crop of main harm has cucumber, peanut, paddy rice, soybean, wheat, tobacco, potato, oranges and tangerines, corn etc.Soybean cyst nematode, also known as root nematode of soybean, is one of Major Diseases of Soybean production, has large area to occur in the U.S. of main Soybean production state, Brazil, Argentina and China.Soybean cyst nematode, generally can make the soybean underproduction 10% ~ 20%, and time serious, the underproduction 70% ~ 90%, even has no harvest.
At present, adopting chemical pesticide to carry out preventing and treating is one of approach tackling root-knot nematode and Cyst nematode harm, along with the efficient nematode medicament phonamiphos of high poison, carbofuran, terbufos restriction or prohibit the use, the control of root-knot nematode relies on Avermectin thus as single medicament, and the resistance of nematode continues to increase.In order to delay root-knot nematode, Cyst nematode to the resistance of Avermectin, be solve its resistance problem simply and efficiently approach by itself and the agriculture chemical compounding of different mechanism of action.
Summary of the invention
The object of the invention is to, for the problem in above-mentioned agricultural production, provide a kind of granular formulation for killing nematode containing Avermectin and trichoderma harzianum, medicament high-efficiency low-toxicity, significantly improves the preventive effect of root-knot nematode, Cyst nematode, reduces agricultural chemicals mu input cost.
The technical solution used in the present invention is: a kind of granular formulation for killing nematode containing Avermectin and trichoderma harzianum, comprises active component and adjuvant.
Described active component is made up of Avermectin and trichoderma harzianum, female medicine that trichoderma harzianum is 30,000,000,000 cfu/ grams with content is set as 100% female medicine, the weight ratio of Avermectin and trichoderma harzianum is 2-15:1-5, Avermectin content is in the composition 0.2 ~ 1.5%, and trichoderma harzianum content is in the composition 0.3 ~ 3.0 hundred million CFU/ gram.
Described adjuvant comprise filler, binding agent, dispersant, wetting agent, disintegrant, sustained release agent, stabilizing agent or other be of value to the active component material that plays of stable and drug effect in the formulation, be all conventional in agriculture field preparation or allow the various compositions that use.
The present invention has the following advantages and significant technique effect:
(1) Avermectin and trichoderma harzianum Synergistic, use with in the reasonable scope, can reduce the amount of application of each single dose, is conducive to reducing drug cost and reducing environmental pollution.
(2) trichoderma harzianum and Avermectin mixture, effectively can delay the resistance of nematode to Avermectin, improves the life cycle of medicament.
(3) trichoderma harzianum can not only effectively preventing nematode, can improve the microenvironment of soil simultaneously, make plant root growth more healthy, have good control efficiency to the root rot in soil-borne disease, southern blight, damping off, damping off.
Embodiment
Be described further technical scheme of the present invention and technique effect below by way of specific embodiment, percentage of the present invention is all weight percentage.
One, indoor joint toxicity measuring test
The indoor joint toxicity measuring test of Avermectin, trichoderma harzianum and mixed agent thereof, 5 concentration are diluted by active constituent content gradient series with Avermectin, trichoderma harzianum and mixed agent thereof, compare with sterile water, test with tomato root-knot eelworm second instar larvae as examination worm, blank is set, Avermectin, trichoderma harzianum single dose and mixture preparation thereof are adopted dip method, carries out toxicity test and do statistical analysis.
Evaluate the synergistic effect of pharmacy mix with the co-toxicity coefficient method (CTC) of the abundant method of Sun Yun, namely CTC≤80 are antagonism, and 80<CTC<120 is summation action, and CTC >=120 are synergistic effect.Statistics is as shown in table 1:
Table 1 Avermectin, trichoderma harzianum and mixed agent thereof are to the Toxicity Determination table of tomato root-knot eelworm
The measurement result of indoor joint toxicity measuring test shows, when the mixed ratio of Avermectin and trichoderma harzianum is within the scope of 2-15:1-5, co-toxicity coefficient (CTC) is all greater than 120, and namely synergistic effect is obvious; When the mixed ratio of Avermectin and trichoderma harzianum is 1:10, co-toxicity coefficient (CTC) is 114.5, and both mixtures are summation action.
Two, product embodiments
Embodiment 1:0.2% Avermectin 0.3 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to Avermectin and trichoderma harzianum is 2:1, takes the former medicine 0.21kg of 95% Avermectin, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.1kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 1.5kg, carboxymethyl cellulose 2.5kg, kaolin complements to 100kg.
Embodiment 2:0.2% Avermectin 0.5 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to Avermectin and trichoderma harzianum is 6:5, take the former medicine 0.21kg of 95% Avermectin, 30000000000 cfu/ grams of Trichoderma harzianum starter medicine 0.17kg, naphthalenesulfonate formaldehyde condensation compound 2.5kg, lauryl sodium sulfate 1.5kg, polyvinyl alcohol 2.5kg, attapulgite 5kg, bentonite complements to 100kg.
Embodiment 3:0.5% Avermectin 1.0 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to Avermectin and trichoderma harzianum is 3:2, take the former medicine 0.53kg of 95% Avermectin, 30000000000 cfu/ grams of Trichoderma harzianum starter medicine 0.33kg, carboxymethyl cellulose 2.5kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 1.5kg, attapulgite 15kg, kaolin complements to 100kg.
Embodiment 4:1.0% Avermectin 1.0 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to Avermectin and trichoderma harzianum is 3:1, takes the former medicine 1.05kg of 95% Avermectin, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.33kg, naphthalenesulfonate formaldehyde condensation compound 1.5kg, lauryl sodium sulfate 2.5kg, polyethylene glycol 2.0kg, diatomite supplies 100kg.
Embodiment 5:0.5% Avermectin 0.6 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to Avermectin and trichoderma harzianum is 5:2, takes the former medicine 0.53kg of 95% Avermectin, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.2kg, lauryl sodium sulfate 2.5kg, carboxymethyl cellulose 2.5kg, diatomite 15kg, bentonite supplies 100kg.
Preparation method: mixed by the recipe ingredient described in above-described embodiment 1-5, pulverizes, stirs, then use screw extruder pelletizer granulation after adding water-wet, and sieving after drying and obtaining containing active component is the granule of Avermectin and trichoderma harzianum.
Three, products application embodiment
Embodiment 1:0.2% Avermectin 0.5 hundred million cfu/ gram of trichoderma harzianum granule is used for the control of cucumber root-knot nematode
(1) experimental scheme
Using 0.2% Avermectin 0.5 hundred million cfu/ gram of trichoderma harzianum granule as experimental group 1#, amount of application is 10 kgs/acre; Respectively with 0.5% Abamectin 1# as a control group, amount of application is 15kg/ mu; 1.5 hundred million cfu/ grams of trichoderma harzianum granule 2# as a control group, amount of application is respectively 20 kgs/acre, arranges 1 blank group.Above-mentioned process is used cucumber vegetative growth phase, within after medicine 30 days, 50 days, carries out cucumber root-knot nematode respectively and to cause harm investigation, carry out determination of yield during results.
(2) result of the test:
After medicine 30 days, experimental group 1#, control group 1#, the Rectifying efficacies of control group 2# to cucumber root-knot nematode respectively were: 87.6%, 72.2% and 68.5%.After medicine 50 days, the Rectifying efficacies of experimental group 1#, control group 1#, control group 2# respectively was 85.9%, 70.4% and 67.4%.Carry out determination of yield statistics between harvest time, experimental group 1#, control group 1#, control group 2# increase by 68.3%, 46.2% and 40.3% than blank respectively
Find out from field trial, after Avermectin and trichoderma harzianum rational mixture, mixed agent is obvious to the control synergy of Cucumber root-knot nematode disease, has both reduced the usage amount of active ingredient, and has shown again the field efficacy being better than single dose.
Embodiment 2:0.5% Avermectin 1.0 hundred million cfu/ gram of trichoderma harzianum granule is used for the control of soybean cyst nematode Heterodera glycines
(1) experimental scheme: select the plot that first crop has serious Cyst nematode to endanger, using 0.5% Avermectin 1.0 hundred million cfu/ gram of trichoderma harzianum granule as experimental group 1#, amount of application is 5 kgs/acre; Respectively with 0.5% Abamectin 1# as a control group, 1 amount of application is 15kg/ mu; 3.0 hundred million cfu/ grams of trichoderma harzianum granule 2# as a control group, amount of application is respectively 15 kgs/acre, arranges 1 blank group.Above-mentioned process is carried out medicament base and is executed during soybean planting, and within 50 days after medicine, carry out result investigation, harvest time carries out determination of yield.
(2) result of the test:
After medicine 50 days, experimental group 1#, control group 1#, the preventive effect of control group 2# to soybean cyst nematode Heterodera glycines respectively were: 78.6%, 64.2% and 59.6%.Carry out output statistics between harvest time, experimental group 1#, control group 1#, control group 2# increase by 72.3%, 48.2% and 46.5% than blank respectively.
After Avermectin and trichoderma harzianum, synergistic effect is obvious, and to the preventive effect of soybean cyst nematode Heterodera glycines significantly better than single dose, output increases obviously than blank.
Claims (2)
1. one kind contains the granular formulation for killing nematode of Avermectin and trichoderma harzianum, be made up of active component and adjuvant, it is characterized in that, described active component is made up of Avermectin and trichoderma harzianum, female medicine that trichoderma harzianum is 30,000,000,000 cfu/ grams with content is set as 100% female medicine, and the weight ratio of Avermectin and trichoderma harzianum is 2-15:1-5.
2. the granular formulation for killing nematode containing Avermectin and trichoderma harzianum according to claim 1, it is characterized in that, Avermectin content is in the composition 0.2 ~ 1.5%, and trichoderma harzianum content is in the composition 0.3 ~ 3.0 hundred million CFU/ gram.
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| CN201510810050.0A CN105284907B (en) | 2015-11-19 | 2015-11-19 | A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum |
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| CN201510810050.0A CN105284907B (en) | 2015-11-19 | 2015-11-19 | A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum |
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| CN105284907B CN105284907B (en) | 2017-11-07 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108338184A (en) * | 2018-03-12 | 2018-07-31 | 甘肃农业大学 | A kind of mematocide |
| CN110903138A (en) * | 2019-11-11 | 2020-03-24 | 辽宁丰园肥业有限公司 | Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests |
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| CN101182468A (en) * | 2007-11-08 | 2008-05-21 | 沈阳农业大学 | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof |
| US20100034792A1 (en) * | 2006-06-19 | 2010-02-11 | The Regents Of The University Of California | Combinations of biological control agents with a nematicidal seed coating |
| CN102077846A (en) * | 2011-01-21 | 2011-06-01 | 佛山市盈辉作物科学有限公司 | Nematoid-killing composition containing abamectin and paecilomyces lilacinus |
| CN103283786A (en) * | 2012-08-07 | 2013-09-11 | 上海交通大学 | Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt |
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2015
- 2015-11-19 CN CN201510810050.0A patent/CN105284907B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100034792A1 (en) * | 2006-06-19 | 2010-02-11 | The Regents Of The University Of California | Combinations of biological control agents with a nematicidal seed coating |
| CN101182468A (en) * | 2007-11-08 | 2008-05-21 | 沈阳农业大学 | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof |
| CN102077846A (en) * | 2011-01-21 | 2011-06-01 | 佛山市盈辉作物科学有限公司 | Nematoid-killing composition containing abamectin and paecilomyces lilacinus |
| CN103283786A (en) * | 2012-08-07 | 2013-09-11 | 上海交通大学 | Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt |
Non-Patent Citations (1)
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| 王中康 等: "《现代生物农药100问》", 31 October 2014 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108338184A (en) * | 2018-03-12 | 2018-07-31 | 甘肃农业大学 | A kind of mematocide |
| CN110903138A (en) * | 2019-11-11 | 2020-03-24 | 辽宁丰园肥业有限公司 | Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests |
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| CN105284907B (en) | 2017-11-07 |
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