CN105284907B - A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum - Google Patents
A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum Download PDFInfo
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- CN105284907B CN105284907B CN201510810050.0A CN201510810050A CN105284907B CN 105284907 B CN105284907 B CN 105284907B CN 201510810050 A CN201510810050 A CN 201510810050A CN 105284907 B CN105284907 B CN 105284907B
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- avm
- trichoderma harzianum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The present invention relates to a kind of granular formulation for killing nematode containing AVM and trichoderma harzianum, its active component is made up of AVM and trichoderma harzianum, trichoderma harzianum is set as 100% female medicine by 30,000,000,000 cfu/ grams of female medicines of content, and the weight ratio of AVM and trichoderma harzianum is 2 15:15, the content of AVM in the composition is 0.2~1.5%, and the content of trichoderma harzianum in the composition is 0.3~3.0 hundred million CFU/ grams.The present invention possesses synergistic function, can not only effectively preventing nematode, while the microenvironment of soil can be improved, make plant root growth more healthy, have preferable prevention effect to the root rot in soil-borne disease, southern blight, samping off, damping-off.The present invention is used with the reasonable scope, can reduce the amount of application of each single dose, is effectively delayed nematode to the resistance of AVM, is improved the usage cycles of medicament, and advantageously reduces drug cost and reduction environmental pollution.
Description
Technical field
It is specifically a kind of to kill nematode particle containing AVM and trichoderma harzianum the present invention relates to technical field of pesticide
Agent.
Background technology
Root-knot nematode and SCN, are to endanger two most heavy class nematodes to agricultural production, the crop of main harm has
Cucumber, peanut, paddy rice, soybean, wheat, tobacco, potato, citrus, corn etc..Soybean cyst nematode is also known as Soybean Root nematode
Disease, is one of Major Diseases of Soybean production, has large area in the U.S. of main Soybean production state, Brazil, Argentina and China
Occur.Soybean cyst nematode, can typically make the soybean underproduction 10%~20%, the underproduction 70%~90% when serious, or even total crop failure.
At present, it is to tackle one of approach that root-knot nematode and SCN endanger to carry out preventing and treating using chemical pesticide, with
The efficient nematode medicament phonamiphos of high poison, carbofuran, the limitation of terbufos are prohibitted the use of, and thus the preventing and treating of root-knot nematode relies on
AVM is continued to increase as single medicament, the resistance of nematode.In order to delay root-knot nematode, SCN to AVM
Resistance, is to solve its resistance problem simply and efficiently approach by itself and the agriculture chemical compounding of different mechanism of action.
The content of the invention
It is an object of the present invention to which the problem of being directed in above-mentioned agricultural production is wooden containing AVM and Ha Ci there is provided one kind
The granular formulation for killing nematode of mould, medicament high-efficiency low-toxicity significantly improves root-knot nematode, the preventive effect of SCN, and reduction agricultural chemicals mu is thrown
Enter cost.
The technical solution used in the present invention is:A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum, bag
Include active component and adjuvant.
Described active component is made up of AVM and trichoderma harzianum, and trichoderma harzianum is using content as 30,000,000,000 cfu/
Gram female medicine be set as 100% female medicine, the weight ratio of AVM and trichoderma harzianum is 2-15:1-5, AVM is in combination
Content in thing is 0.2~1.5%, and the content of trichoderma harzianum in the composition is 0.3~3.0 hundred million CFU/ grams.
Described adjuvant includes filler, binding agent, dispersant, wetting agent, disintegrant, sustained release agent, stabilizer or other
Be beneficial to the material that active component is stablized in the formulation and drug effect is played, commonly used or allow to use in agriculture field preparation
Various composition.
The present invention has advantages below and significant technique effect:
(1) AVM and trichoderma harzianum Synergistic, are used with the reasonable scope, can reduce the administration of each single dose
Amount, advantageously reduces drug cost and reduction environmental pollution.
(2) trichoderma harzianum and AVM mixture, can effectively delay nematode to the resistance of AVM, improve medicament
Usage cycles.
(3) trichoderma harzianum can not only effectively preventing nematode, while the microenvironment of soil can be improved, give birth to root system of plant
Length is more healthy, has preferable prevention effect to the root rot in soil-borne disease, southern blight, samping off, damping-off.
Embodiment
Technical scheme and technique effect are described further below by way of specific embodiment, institute of the present invention
The percentage stated is weight percentage.
First, indoor joint toxicity measuring test
The indoor joint toxicity measuring test of AVM, trichoderma harzianum and its mixed agent, with AVM, breathes out thatch wood
Mould and its mixed agent dilute 5 concentration by active constituent content gradient series, are compared with sterilized water, test with tomato root knot
Nematode second instar larvae is test worm, sets blank control, by AVM, trichoderma harzianum single dose and its mixture preparation using leaching worm
Method, carries out toxicity test and does statistical analysis.
The synergistic effect of pharmacy mix, i.e. CTC≤80 are evaluated with the co-toxicity coefficient method (CTC) of the abundant methods of Sun Yun for antagonism to make
With 80<CTC<120 be summation action, and CTC >=120 are synergistic effect.Statistical result is as shown in table 1：
The Toxicity Determination table of the AVM of table 1, trichoderma harzianum and its mixed agent to tomato root-knot eelworm
The measurement result of indoor joint toxicity measuring test shows that the mixed ratio of AVM and trichoderma harzianum is 2-
15:When in the range of 1-5, it is obvious that co-toxicity coefficient (CTC) is all higher than 120, i.e. synergistic effect；AVM and trichoderma harzianum it is mixed
It is 1 with ratio:When 10, co-toxicity coefficient (CTC) is 114.5, and both mixtures are summation action.
2nd, product embodiments
Embodiment 1：The cfu/ grams of trichoderma harzianum granule of 0.2% AVM 0.3 hundred million
Formula：Weight ratio according to AVM and trichoderma harzianum is 2:1, weigh 95% the former medicine of AVM
0.21kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.1kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 1.5kg, carboxylic first
Base cellulose 2.5kg, kaolin complements to 100kg.
Embodiment 2：The cfu/ grams of trichoderma harzianum granule of 0.2% AVM 0.5 hundred million
Formula：Weight ratio according to AVM and trichoderma harzianum is 6:5, weigh 95% the former medicine of AVM
0.21kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.17kg, naphthalenesulfonate formaldehyde condensation compound 2.5kg, lauryl sodium sulfate
1.5kg, polyvinyl alcohol 2.5kg, attapulgite 5kg, bentonite complements to 100kg.
Embodiment 3：The cfu/ grams of trichoderma harzianum granule of 0.5% AVM 1.0 hundred million
Formula：Weight ratio according to AVM and trichoderma harzianum is 3:2, weigh 95% the former medicine of AVM
0.53kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.33kg, carboxymethyl cellulose 2.5kg, lauryl sodium sulfate 2.5kg,
Polyvinyl alcohol 1.5kg, attapulgite 15kg, kaolin complements to 100kg.
Embodiment 4：The cfu/ grams of trichoderma harzianum granule of 1.0% AVM 1.0 hundred million
Formula：Weight ratio according to AVM and trichoderma harzianum is 3:1, weigh 95% the former medicine of AVM
1.05kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.33kg, naphthalenesulfonate formaldehyde condensation compound 1.5kg, lauryl sodium sulfate
2.5kg, polyethylene glycol 2.0kg, diatomite supplies 100kg.
Embodiment 5：The cfu/ grams of trichoderma harzianum granule of 0.5% AVM 0.6 hundred million
Formula：Weight ratio according to AVM and trichoderma harzianum is 5:2, weigh 95% the former medicine of AVM
0.53kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.2kg, lauryl sodium sulfate 2.5kg, carboxymethyl cellulose 2.5kg,
Diatomite 15kg, bentonite supplies 100kg.
Preparation method：Recipe ingredient described in above-described embodiment 1-5 is well mixed, crushed, is fully stirred after the wetting that adds water
Mix uniform, then granulated with screw extruder pelletizer, it is AVM and Trichoderma harzianum that sieving, which is produced containing active component, after drying
The granule of bacterium.
3rd, products application embodiment
Embodiment 1：The cfu/ grams of trichoderma harzianum granule of 0.2% AVM 0.5 hundred million is used for cucumber root-knot nematode
Preventing and treating
(1) experimental design
Using the cfu/ grams of trichoderma harzianum granule of 0.2% AVM 0.5 hundred million as test group 1#, amount of application is 10 public
Pound/acre；Respectively with 0.5% Abamectin 1# as a control group, amount of application is 15kg/ mus；1.5 hundred million cfu/ Ke Haci wood
Mold particles agent 2# as a control group, amount of application is respectively 20 kgs/acre, sets 1 blank control group.Above-mentioned processing is sought in cucumber
30 days after health chronic administration, medicine, cucumber root-knot nematode being carried out respectively within 50 days and being caused harm investigation, determination of yield is carried out during harvest.
(2) result of the test：
30 days after medicine, test group 1#, control group 1#, control group 2# distinguish the Rectifying efficacies of cucumber root-knot nematode successively
For：87.6%th, 72.2% and 68.5%.50 days after medicine, test group 1#, control group 1#, control group 2# Rectifying efficacies divide successively
Wei 85.9%, 70.4% and 67.4%.Determination of yield statistics, test group 1#, control group 1#, control group 2# are carried out during harvest
Respectively than blank control increase by 68.3%, 46.2% and 40.3%
Find out from field test, after AVM and trichoderma harzianum rational mixture, mixed agent is to cucumber root-knot nematode
The preventing and treating synergy of disease substantially, had both reduced the usage amount of active ingredient, the field efficacy better than single dose is shown again.
Embodiment 2：The cfu/ grams of trichoderma harzianum granule of 0.5% AVM 1.0 hundred million is used for soybean cyst nematode Heterodera glycines
Preventing and treating
(1) experimental design：Selection first crop has the plot of serious SCN harm, by 0.5% AVM 1.0 hundred million
Cfu/ grams of trichoderma harzianum granule is as test group 1#, and amount of application is 5 kgs/acre；Respectively with 0.5% Abamectin
1# as a control group, 1 amount of application is 15kg/ mus；3.0 hundred million cfu/ grams of trichoderma harzianum granules 2#, amount of application as a control group
It is respectively 15 kgs/acre, 1 blank control group is set.It is above-mentioned handle during soybean planting carry out medicament base apply, in 50 days after medicine
Result investigation is carried out, harvest time carries out determination of yield.
(2) result of the test：
50 days after medicine, test group 1#, control group 1#, control group 2# respectively are to the preventive effect of soybean cyst nematode Heterodera glycines：
78.6%th, 64.2% and 59.6%.Output statistics is carried out during harvest, test group 1#, control group 1#, control group 2# are respectively than sky
White control increase by 72.3%, 48.2% and 46.5%.
Substantially, the preventive effect to soybean cyst nematode Heterodera glycines is significantly better than single dose for synergistic effect after AVM and trichoderma harzianum,
Yield is more obvious than blank control increase.
Claims (2)
1. a kind of granular formulation for killing nematode containing AVM and trichoderma harzianum, is made up of, its feature active component and adjuvant
It is, described active component is made up of AVM and trichoderma harzianum, trichoderma harzianum is using content as 30,000,000,000 cfu/ grams
Female medicine is set as 100% female medicine, and the weight ratio of AVM and trichoderma harzianum is 2-15:1-5.
2. the granular formulation for killing nematode according to claim 1 containing AVM and trichoderma harzianum, it is characterised in that AVM hereinafter
The content of rhzomorph in the composition is 0.2~1.5%, and the content of trichoderma harzianum in the composition is 0.3~3.0 hundred million CFU/
Gram.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510810050.0A CN105284907B (en) | 2015-11-19 | 2015-11-19 | A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum |
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| CN201510810050.0A CN105284907B (en) | 2015-11-19 | 2015-11-19 | A kind of granular formulation for killing nematode containing AVM and trichoderma harzianum |
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| CN105284907B true CN105284907B (en) | 2017-11-07 |
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| CN108338184A (en) * | 2018-03-12 | 2018-07-31 | 甘肃农业大学 | A kind of mematocide |
| CN110903138A (en) * | 2019-11-11 | 2020-03-24 | 辽宁丰园肥业有限公司 | Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182468A (en) * | 2007-11-08 | 2008-05-21 | 沈阳农业大学 | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof |
| CN102077846A (en) * | 2011-01-21 | 2011-06-01 | 佛山市盈辉作物科学有限公司 | Nematoid-killing composition containing abamectin and paecilomyces lilacinus |
| CN103283786A (en) * | 2012-08-07 | 2013-09-11 | 上海交通大学 | Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI422328B (en) * | 2006-06-19 | 2014-01-11 | Univ California | Combinations of biological control agents with a nematicidal seed coating |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182468A (en) * | 2007-11-08 | 2008-05-21 | 沈阳农业大学 | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof |
| CN102077846A (en) * | 2011-01-21 | 2011-06-01 | 佛山市盈辉作物科学有限公司 | Nematoid-killing composition containing abamectin and paecilomyces lilacinus |
| CN103283786A (en) * | 2012-08-07 | 2013-09-11 | 上海交通大学 | Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt |
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