CN105284906B - A kind of composition pesticide for preventing and treating soybean cyst nematode - Google Patents
A kind of composition pesticide for preventing and treating soybean cyst nematode Download PDFInfo
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- CN105284906B CN105284906B CN201510800385.4A CN201510800385A CN105284906B CN 105284906 B CN105284906 B CN 105284906B CN 201510800385 A CN201510800385 A CN 201510800385A CN 105284906 B CN105284906 B CN 105284906B
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- trichoderma harzianum
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- lythidathion
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of composition pesticide for preventing and treating soybean cyst nematode, active component in composition is made up of lythidathion, trichoderma harzianum and amino-oligosaccharide, described trichoderma harzianum is set to 100% female medicine by 30,000,000,000 CFU/ grams of female medicines of content, and lythidathion, trichoderma harzianum, the weight ratio of amino-oligosaccharide are 15 30:1‑3:1‑5.Active component mixture Synergistic used in the present invention, not only there is preferable prevention effect to root-knot nematode, SCN, the root fungi disease such as energy effectively preventing root rot, samping off, damping-off simultaneously, effectively avoid the Combined Infection of root fungi disease and nematodiasis；It to crop safety, can spread fertilizer over the fields, base is applied, granule is prepared into, using more convenient.
Description
Technical field
The present invention relates to technical field of pesticide, specifically a kind of composition pesticide for preventing and treating soybean cyst nematode.
Background technology
Root knot nematode disease and Cyst nematode, it is two most heavy class nematodiasis of the harm to agricultural production, main harm
Crop has cucumber, peanut, rice, soybean, wheat, tobacco, potato, citrus, corn etc..Eelworm harm can typically make crop cut
Production 10%~20%, the underproduction 70%~90% when serious, or even total crop failure.
Soil continuous cropping, with the root rot of fungal attack, droop, damping-off, samping off and the root knot as caused by nematode
Nematodiasis, Cyst nematode can all occur during crop-planting.Soil nematodes has substantial connection with crop root disease, soil
Earth nematode can cause the wound of root system of plant, favourable infection process and aggravate disease, often nematode sends out simultaneously with fungal disease
It is raw.Li Maosheng, tight tertiary equality people deliver《Wilt and root-knot nematode are studied cucumber Combined Infection》Confirm droop
Sharp fusarium (Fusariumoxysporum) and Meloidogyne incognita on cucumber can Combined Infection, cause heavy losses.
At present in agricultural production, medicament can not typically prevent and treat the fungal disease and root knot nematode disease of crop root simultaneously, if
The preventing and treating of different times is carried out with two kinds of different medicaments simultaneously, substantial amounts of labour is not only expended, also increase drug cost.
Therefore, it is necessary to a kind of pesticide control, while eelworm harm prevent and treat energy effectively preventing root rot, samping off,
The silborne fungal diseases such as damping-off.
The content of the invention
It is an object of the present invention to the problem of in above-mentioned agricultural production, there is provided one kind preventing and treating soybean cyst nematode
Composition pesticide, medicament high-efficiency low-toxicity can effectively prevent and treat the harm of root-knot nematode, SCN, at the same can prevent and treat root rot,
The root fungi disease such as samping off, damping-off.
The technical solution used in the present invention is：A kind of composition pesticide for preventing and treating soybean cyst nematode, including activity
Composition and adjuvant, wherein active component include：
(1) lythidathion, chemical name are:O- ethyl-S- sec-butyl -2- oxo -1,3- thiazolidine -3- base phosphonothiolic acids
Ester.It is a kind of organic phosphates nematicide, has and tag and stomach poison function, crop root is drawn in energy kills and invaded root of the crop
Portion forms the root-knot nematode of root nodule.
(2) trichoderma harzianum, it is a kind of microbial bactericide, the root disease of crop can be suppressed.Trichoderma harzianum is not
It is only capable of suppressing the growth of germ, improves the microenvironment of root system, strengthen the growing way and resistance against diseases of plant, also have one to root-knot nematode
Fixed prevention effect.
(3) amino-oligosaccharide, chemical name are oligomeric-D Glucosamines, be microbial metabolism extraction one kind have it is anti-
The bactericide of disease effect, can excite the expression of plant gene, produce the chitinase with antibacterial action, dextranase, protect element and
PR albumen；The growth of germ can be suppressed simultaneously, moreover it is possible to stimulate the growth of crop.Amino-oligosaccharide is not only to crop root rot, sudden
Disease, damping-off have preferable effect, also there is preferable preventive and therapeutic effect to root-knot nematode.
Described active component, trichoderma harzianum are set to 100% female medicine, thiazole by 30,000,000,000 CFU/ grams of female medicines of content
Phosphorus, trichoderma harzianum, the weight ratio of amino-oligosaccharide are 15-30:1-3:1-5, the content of lythidathion in the composition be 2.5~
10%, the content of trichoderma harzianum in the composition is 0.5~3.0 hundred million CFU/ grams；The content of amino-oligosaccharide in the composition
For 0.5~2.5%.
Described adjuvant also include filler, binding agent, dispersant, wetting agent, disintegrant, sustained release agent, stabilizer or its
It is beneficial to the material that active ingredient is stablized in the formulation and drug effect plays.
A kind of composition pesticide of described preventing and treating nematode is prepared into the granule in agricultural production, using in soil-borne disease
Root rot, samping off, damping-off, droop, root knot nematode disease and Cyst nematode.
The present invention has advantages below and significant technique effect:
(1) lythidathion, trichoderma harzianum and amino-oligosaccharide three's mixture Synergistic of the invention, within the specific limits
The mixed amount of application for advantageously reducing each single dose, help to reduce pesticide cost.
(2) present invention not only has preferable prevention effect to root-knot nematode, SCN, while can effectively preventing root-rot
The root fungi disease such as disease, samping off, damping-off, effectively avoid the Combined Infection of root fungi disease and nematodiasis.
(3) medicament is to crop safety, can spread fertilizer over the fields, base is applied, and granule is prepared into, using more convenient.
Embodiment
Technical scheme and technique effect are described further below by way of specific embodiment, except special sound
Bright outer, percentage of the present invention is weight percentage.
First, indoor joint toxicity measuring test
Lythidathion, trichoderma harzianum, the indoor joint toxicity measuring test of amino-oligosaccharide and its mixed agent, with thiazole
Phosphorus, trichoderma harzianum, amino-oligosaccharide and its mixed agent dilute 5 concentration by active constituent content gradient series, use sterilized water
Compare, test using soybean cyst nematode Heterodera glycines second instar larvae as test worm, blank control is set, by lythidathion, trichoderma harzianum, amino
Oligosaccharide single dose and mixture use dip method, carry out toxicity test and do statistical analysis.
The synergistic effect of pharmacy mix is evaluated with the co-toxicity coefficient method (CTC) of the abundant methods of Sun Yun, i.e., CTC≤80 are that antagonism is made
With 80<CTC<120 be summation action, and CTC >=120 are synergistic effect.Statistical result is as shown in table 1：
The Toxicity Determination of the lythidathion of table 1, trichoderma harzianum, amino-oligosaccharide and its mixed agent to soy bean cyst roundworm
Table
The measurement result of indoor joint toxicity measuring test shows, when lythidathion, trichoderma harzianum and amino-oligosaccharide
Mixed ratio is 15-30:1-3:During 1-5, co-toxicity coefficient (CTC) is all higher than 120, has obvious synergistic effect；When lythidathion,
The mix ratio of trichoderma harzianum and amino-oligosaccharide is 1:5:When 1, co-toxicity coefficient is less than 80, and mutual mixture produces antagonism.
2nd, product embodiments
Embodiment 1：The cfu/ grams of amino-oligosaccharide granule of trichoderma harzianum 0.5% of 2.5% lythidathion 0.5 hundred million
Formula：Weight ratio according to lythidathion, trichoderma harzianum and amino-oligosaccharide is 15:1:3, weigh 95% lythidathion
Active compound 2.63kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.17kg, 85% amino-oligosaccharide active compound 0.59kg, naphthalene sulfonate
Formaldehyde condensation products 1.5kg, lauryl sodium sulfate 3.5kg, polyvinyl alcohol 1.5kg, attapulgite 15kg, bentonite complement to
100kg。
Embodiment 2：The cfu/ grams of amino-oligosaccharide granule of trichoderma harzianum 0.5% of 5.0% lythidathion 1.0 hundred million
Formula：Weight ratio according to lythidathion, trichoderma harzianum and amino-oligosaccharide is 30:2:3, weigh 95% lythidathion
Active compound 5.26kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.33kg, 85% amino-oligosaccharide active compound 0.59kg, naphthalene sulfonate
Formaldehyde condensation products 3.5kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 1.5kg, attapulgite 25kg, kaolin complement to
100kg。
Embodiment 3：The cfu/ grams of amino-oligosaccharide granule of trichoderma harzianum 2.5% of 10.0% lythidathion 3.0 hundred million
Formula：Weight ratio according to lythidathion, trichoderma harzianum and amino-oligosaccharide is 20:2:5, weigh 95% lythidathion
Active compound 10.53kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 1.0kg, 85% amino-oligosaccharide active compound 2.94kg, dodecyl
Sodium sulphate 2.5kg, carboxymethyl cellulose 2.0kg, diatomite 25kg, bentonite complement to 100kg.
Preparation method：Recipe ingredient described in above-described embodiment 1-3 is well mixed, crushed, adds water fully to be stirred after soaking
Mix uniformly, then be granulated with screw extruder pelletizer, sieve and produced containing lythidathion, trichoderma harzianum and amino-oligosaccharide after drying
Granule.
3rd, Application Example
Embodiment 1：Should by the cfu/ grams of amino-oligosaccharide granule of trichoderma harzianum 0.5% of 2.5% lythidathion 0.5 hundred million
For soybean cyst nematode field experiment.
Experimental design：During soybean planting, usually root rot and Cyst nematode occurs simultaneously, selects first crop seriously ill
Plot, using the cfu/ grams of amino-oligosaccharide granule of trichoderma harzianum 0.5% of 2.5% lythidathion 0.5 hundred million as test group
1#, amount of application are 5 kgs/acre；With 5% lythidathion granule, 1#, 1 amount of application are 10kg/ mus as a control group respectively；5.0 hundred million
2#, amount of application are respectively 15 kgs/acre to cfu/ grams of trichoderma harzianum granule as a control group；2.5% amino-oligosaccharide granule,
Amount of application is 15kg/ mus, sets 1 blank control group.
It is above-mentioned handle during soybean planting carry out medicament base apply, after medicine 50 days carry out result investigation, harvest time carry out
Determination of yield, result of the test are as shown in the table：
| Test process | To the Rectifying efficacies (%) of soybean cyst nematode Heterodera glycines | Yield rate of growth (%) |
| Test group 1# | 76.42 | 87.92 |
| Control group 1# | 59.83 | 63.48 |
| Control group 2# | 56.75 | 65.73 |
| Control group 3# | 52.72 | 58.57 |
Find out from experiment, after lythidathion, trichoderma harzianum and amino-oligosaccharide carry out rational mixture, mixed agent is to soybean
The synergy of Cyst nematode is obvious, has both reduced the usage amount of active ingredient, shows the field efficacy better than single dose again.
Embodiment 2：The cfu/ grams of amino-oligosaccharide granule application of trichoderma harzianum 0.5% of 5.0% lythidathion 1.0 hundred million
In preventing and treating cucumber root line nematode and the field experiment of preventing and treating cucumber samping off.
(1) experimental design：
Continuous cropping cucumber samping off and the concurrent serious plot of root knot nematode disease are selected, by the cfu/ of 5.0% lythidathion 1.0 hundred million
As test group 1#, amount of application is 5 kgs/acre for gram amino-oligosaccharide granule of trichoderma harzianum 0.5%；Respectively with 10% thiophene
1#, amount of application are 10kg/ mus to azoles phosphorus granule as a control group；6.0 hundred million cfu/ grams of trichoderma harzianum granules are as a control group
2#, amount of application are 15kg/ mus；3#, amount of application are respectively 15 kgs/acre to 5% amino-oligosaccharide granule as a control group, set 1
Individual blank control group.
(2) application method：
By experimental design by test group 1#, control group 1#, control group 2#, control group 3# medicament and dosage uniformly mix soil in
Cave is sowed, is planted afterwards in cave is sowed into the cucumber seeds for having urged bud, it is each to handle 100 caves per 3, cave cucumber seedling.Everywhere
Farming operations in reason are consistent.
(3) result is investigated
Cucumber at seedling stage samping off is investigated after 20 days after dispenser, counts incidence；Carry out cucumber root tie lines within 40 days after medicine
Parasitosis is investigated, and result of the test is as shown in the table：
| Test process | 20 days after medicine, to the preventive effect of cucumber samping off, % | 40 days after medicine, to the preventive effect of cucumber root-knot nematode, % |
| Test group 1# | 74.5 | 92.6 |
| Control group 1# | 15.7 | 73.4 |
| Control group 2# | 62.5 | 68.5 |
| Control group 3# | 61.8 | 61.2 |
From result of the test, after lythidathion, trichoderma harzianum and amino-oligosaccharide compounding, built agent is prevented root-knot nematode
It is obvious to imitate synergy, had both reduced the usage amount of active ingredient, and had shown the field efficacy better than single dose again；Built agent is to sudden simultaneously
Disease also has preferable prevention effect.
Claims (3)
1. a kind of composition pesticide for preventing and treating soybean cyst nematode, it is characterised in that the active component in composition is by thiazole
Phosphorus, trichoderma harzianum and amino-oligosaccharide composition, described trichoderma harzianum are set to by 30,000,000,000 CFU/ grams of female medicines of content
100% female medicine, lythidathion, trichoderma harzianum, the weight ratio of amino-oligosaccharide are 15-30:1-3:1-5.
2. the composition pesticide of preventing and treating soybean cyst nematode according to claim 1, it is characterised in that lythidathion is in group
Content in compound is 2.5~10%, and the content of trichoderma harzianum in the composition is 0.5~3.0 hundred million CFU/ grams；Amino-oligosacchride
The content of element in the composition is 0.5~2.5%.
3. the composition pesticide of preventing and treating soybean cyst nematode according to claim 1, it is characterised in that described combination
Thing adds adjuvant by active component and is prepared into granule.
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| CN201510800385.4A CN105284906B (en) | 2015-11-19 | 2015-11-19 | A kind of composition pesticide for preventing and treating soybean cyst nematode |
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| CN201510800385.4A CN105284906B (en) | 2015-11-19 | 2015-11-19 | A kind of composition pesticide for preventing and treating soybean cyst nematode |
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| CN105284906B true CN105284906B (en) | 2017-11-28 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106035334B (en) * | 2016-06-21 | 2018-08-17 | 佛山市盈辉作物科学有限公司 | A kind of composition pesticide containing amino-oligosaccharide and fludioxonil |
| CN106135291B (en) * | 2016-07-14 | 2019-01-08 | 海南金雨丰生物工程有限公司 | A kind of composition prevented and treated soil-borne disease, improve crop cold resistance |
| CN106259466A (en) * | 2016-07-20 | 2017-01-04 | 陕西西大华特科技实业有限公司 | A kind of bactericidal composition and application thereof |
| CN106508924A (en) * | 2016-10-27 | 2017-03-22 | 佛山市盈辉作物科学有限公司 | Nematicide composition containing fluazaindolizine and amino-oligosaccharin |
| CN106719894A (en) * | 2016-11-25 | 2017-05-31 | 河池市农业科学研究所 | A kind of microbial pesticide for administering Sugarcane Disease |
| CN106857581B (en) * | 2017-02-28 | 2020-06-30 | 广东真格生物科技有限公司 | Nematicidal composition containing trifluromiazinamide |
Citations (4)
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| US6475772B1 (en) * | 2000-03-31 | 2002-11-05 | Council Of Scientific And Industrial Research | Strain of Trichoderma harzianum useful as nematode inhibitor, fungicide and plant growth promoter and a process for the isolation thereof |
| CN101182468A (en) * | 2007-11-08 | 2008-05-21 | 沈阳农业大学 | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof |
| CN102365974A (en) * | 2011-10-28 | 2012-03-07 | 海南正业中农高科股份有限公司 | Agricultural composition for nematode control and its application |
| CN103598210A (en) * | 2013-11-14 | 2014-02-26 | 云南省农业科学院农业环境资源研究所 | Pesticide composition for preventing and controlling nematode |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6475772B1 (en) * | 2000-03-31 | 2002-11-05 | Council Of Scientific And Industrial Research | Strain of Trichoderma harzianum useful as nematode inhibitor, fungicide and plant growth promoter and a process for the isolation thereof |
| CN101182468A (en) * | 2007-11-08 | 2008-05-21 | 沈阳农业大学 | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof |
| CN102365974A (en) * | 2011-10-28 | 2012-03-07 | 海南正业中农高科股份有限公司 | Agricultural composition for nematode control and its application |
| CN103598210A (en) * | 2013-11-14 | 2014-02-26 | 云南省农业科学院农业环境资源研究所 | Pesticide composition for preventing and controlling nematode |
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| "哈茨木霉菌株TRI2的鉴定及其对黄瓜根结线虫的防治作用";马金慧 等;《中国农学通报》;20140805;第30卷(第22期);第263-269页 * |
| "氨基寡糖素(0.5%水剂)对黄瓜根结线虫病的防治试验";郭志刚;《农业科技通讯》;20140317(第3期);第123-124页 * |
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| CN105284906A (en) | 2016-02-03 |
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