CN108338184A - A kind of mematocide - Google Patents
A kind of mematocide Download PDFInfo
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- CN108338184A CN108338184A CN201810198104.6A CN201810198104A CN108338184A CN 108338184 A CN108338184 A CN 108338184A CN 201810198104 A CN201810198104 A CN 201810198104A CN 108338184 A CN108338184 A CN 108338184A
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- avermectin
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- bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The invention belongs to pesticide fields, and in particular to a kind of mematocide containing avermectin and long shoot trichoderma bacterium.Said preparation is made of abamectin solution and long shoot trichoderma bacterium (T.longibrachiatum) bacterium solution, it can significantly improve to wheat cearal cyst nematode toxic action, and there is slow releasing function, the lasting period of pesticide can be extended, said preparation is pollution-free, and being compatible with the environment property is good, and production cost is low, nematode is not likely to produce drug resistance, has the effects that expand prevention spectrum and synergy.
Description
Technical field
The invention belongs to pesticide fields, and in particular to a kind of mematocide containing avermectin and long shoot trichoderma bacterium.
Background technology
Wheat cearal cyst nematode (Heterodera avenae) belongs to plant anchorage parasitic nematode, is to exist in recent years
A kind of disease that China and world wheat major production areas occur extensively.The nematode from 1908 since Britain finds for the first time,
There are 32 countries to report the disease at present, such as only in Victoria of Australia and South Australia area, wheat
Injured area is up to 200 × 104hm2, lead to grain drop in production 23%~50%, the underproduction 73%~89% when serious passes through caused by annual
Ji loss is up to 70,000,000 dollars.In European main cereal crop growing spots, 50% or more field is by Singularity SCAR mark of wheat sporangiocyst
Nematode infects and endangers.In China, wheat cearal cyst nematode was found in 1989 in Hubei for the first time, had proven to the line at present
Worm is distributed widely in the provinces and cities such as Hebei, Henan, Beijing, Hubei, Gansu and Anhui, endangers wheat area up to million hectares or more,
Have become a new problem in China's Wheat Production.
Currently, the prevention to plant nematode diseases is mainly become reconciled year using chemical prevention such as Furadan, ground wire phosphorus, meter Le Er
In winter etc., control effect is up to 90% or more, but it is big with pedo relict height and toxicity, and endangers beneficial microbe in soil
The defects of, and the continuous enhancing of the understanding and environmental consciousness chemical agent endangered with people, the use of chemical pesticide by
Limitation is arrived.Therefore, plant pathogeny line insect biological control is more and more widely paid close attention to, and is prevented especially with parasitic fungi
Cereal Cyst Nematode of Wheat is done harm to, and is current one of the emphasis of biocontrol of nematodes and hot spot both at home and abroad.Trichoderma
(Trichoderma sp.) is a kind of habitat bacterium being distributed widely in soil.According to Zhang Shuwu etc. research shows that long shoot trichoderma
Bacterium (T.longibrachiatum) has apparent parasitic and lethal effect to wheat cearal cyst nematode.In addition, avermectin
It is a kind of never poison class biological pesticide, including plant nematode to agriculturally many pests has certain control effect,
But in agricultural production single use avermectin easily cause wheat cearal cyst nematode drug resistance and effect it is unstable,
And the different mixed preparations of biological agent can make up the deficiency of single dose, can play and expand controlling object, delay nematode
The effects that drug resistance and raising control effect, and said preparation is pollution-free, and being compatible with the environment property is good, and production cost is low.
Invention content
In view of the above-mentioned problems, the present invention provides a kind of mematocide, the preparation is by abamectin solution and length
The volume ratio of branch Trichoderma bacterium solution composition, wherein abamectin solution and long shoot trichoderma bacterium bacterium solution is 1:1-3.
Further, a concentration of 0.1-0.5 μ gmL of the abamectin solution-1, viable bacteria in long shoot trichoderma bacterium bacterium solution
A concentration of 2 × 107cfu·mL-1。
Further, a concentration of 0.1 μ gmL of the abamectin solution-1。
Further, a concentration of 0.2 μ gmL of the abamectin solution-1。
Further, a concentration of 0.3 μ gmL of the abamectin solution-1。
Further, a concentration of 0.4 μ gmL of the abamectin solution-1。
Further, a concentration of 0.5 μ gmL of the abamectin solution-1。
Further, the nematode is wheat cearal cyst nematode.
The beneficial effects of the present invention are：
1. can not only significantly improve to wheat cearal cyst nematode toxic action, and holding for biological pesticide can be extended
The effect phase.
2. being compatible with the environment property is good, production cost is low, and nematode is not likely to produce drug resistance, and with expansion prevention spectrum and collaboration
The effects that synergy.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only the part of the present invention, rather than the whole invented.Based on the embodiments of the present invention, ordinary skill people
The every other embodiment that member is obtained without making creative work, shall fall within the protection scope of the present invention.
The poison of 1 single long shoot trichoderma of embodiment, 2 instar larvae of single avermectin, long shoot trichoderma and avermectin mixture pair
Kill effect one, test material
1, strains tested：Trichoderma longibrachiatum strain was provided by Gansu Agriculture University's Plant Pathology laboratory, and in 2016
It was preserved in Institute of Microorganism, Academia Sinica on November 14, preserving number is CGMCC No.13183, preservation title：Long shoot trichoderma
Trichoderma Longibrachiatum。
2, for trying soil sample：Sick field soil sample picked up from July, 2015 Wheat in Henan Province cereal cyst nematode occur it is more serious
Plot.Five point sampling acquires soil sample, removes surface layer dry ground when fetching earth, takes 0~20cm layers of soil, soil sample is packed into self-styled
Bag, it is spare to take back laboratory.
3, reagent agent：The avermectin that active constituent content is 1.8%, dosage form is missible oil, and generation good science and technology in Zhejiang is limited
Company produces.
Two, test procedure and method
1, the separation of 2 instar larvae of wheat cearal cyst nematode
The separation of sporangiocyst uses " floating method ", and suitable tap water is added into soil sample and stirs evenly, keeps soil sample completely molten
Solution is poured into suspension in the 25 mesh mesh screens in 65 mesh mesh screens after standing 2~3min, is repeated operation 3 times.Incline
Residue on 25 mesh mesh screen of upper layer, residue is collected with slow-flowing stream in clean beaker again, and discards lower sediment
Silt pours into upper layer sample in the funnel for being lined with the double-deck lens wiping paper, then the double-deck lens wiping paper and filtered residue one
And move into clean glass culture dish, and with tweezers or homemade the bamboo stick therefrom fresh and full palm fibre of picking under anatomical lens
It is spare to be placed in 4 DEG C of refrigerators for color sporangiocyst.
Sporangiocyst after 1%NaClO is sterilized is put into the nematode separator for being lined with 2 layers of lens wiping paper, is hatched at 20 DEG C
13~22d is to get to a large amount of 2 instar larvae, and it is Singularity SCAR mark of wheat sporangiocyst line to combine the identification by morphological characters of sporangiocyst and 2 instar larvaes
Worm.Then, it is counted using counting 2 instar larvae of ware pair, makes 2 instar larvae suspension concentrations be 200 mL by preparing-1。
2, the preparation of long shoot trichoderma conidium bacterium solution：
5mL sterile water fully shakings are added into the long shoot trichoderma bacterium for cultivating 6d on PDA plate, make long shoot trichoderma bacterium
Conidium falls off in sterile water, and collection obtains the stoste of conidium bacterium solution, and is counted using blood counting chamber, is diluted to
2.0×107cfu·mL-1It is spare.
3, abamectin solution is prepared：
It is 0.1,0.2,0.3,0.4,0.5 μ gmL through sterile water difference compound concentration-1Abamectin solution is spare.
4, long shoot trichoderma spore bacterium solution and abamectin solution mixture are prepared：
By a concentration of 2.0 × 107cfu·mL-1Long shoot trichoderma spore bacterium solution and above-mentioned 5 concentration gradients avermectin
Solution is 1 by volume respectively:1、2:1、3:1 mixing, marks successively：1 group of mixture, 2 groups of mixture, 3 groups of mixture, and fully shaking
It is set to be uniformly mixed.
5, the measurement of 2 instar larvae toxic action of long shoot trichoderma and avermectin mixture pair
In 96 porocyte culture plates of sterilizing, a concentration of 200 mL of 10 μ L are added per hole-12 instar larvae suspension
With 90 μ L long shoot trichodermas and avermectin mixed liquor, 16 repetitions are each handled, with the single long shoot trichoderma and list of same concentrations
One avermectin is as positive control, and sterile water is as negative control.Experiment carries out at 25 DEG C, and every for 24 hours in microscope
The dead borer population of nematode of lower observation.Nematode in it is stiff it is motionless be dead worm, nematode is worm living in bending wriggling state, at record
2 instar larvae The dead quantity in reason.It calculates its corrected mortality, the lethal dose of 50 and carries out variance analysis.
The death rate (%)=(dead 2 instar larvae quantity/for trying 2 instar larvae quantity) × 100
Corrected mortality (%)=(the processing death rate-control death rate)/(1- compares the death rate) × 100
6, data processing
It is for statistical analysis to data using Excel 2007 and 16.0 softwares of SPSS, and LC is calculated using DPS softwares50。
Three, test results and analysis
The toxic action of 1 single 2 instar larvae of long shoot trichoderma bacterium pair of table
2 instar larvae of single long shoot trichoderma pair has significantly toxic effect：As can be seen from Table 1, using a concentration of 2.0 ×
107cfu·mL-1Long shoot trichoderma conidium bacterium solution after 24h, the death rate and corrected mortality of 2 instar larvaes are respectively
57.14% and 57.14%；The death rate of 2 instar larvaes and corrected mortality are respectively 71.43% and 71.43% after 48h；After 72h
The death rate and corrected mortality of 2 instar larvaes are respectively 81.03% and 79.22%.It can be seen that long shoot trichoderma conidium bacterium
Liquid has significant toxic action to wheat cearal cyst nematode, and toxic effect enhances with the increase of processing time.
The toxic action of 2 single avermectin pair of table, 2 instar larvae
The toxic action of 2 instar larvae of single abamectin solution pair：As can be seen from Table 2 with the increase of concentration and processing
The death rate of the growth of time, 2 instar larvaes gradually increases.Maximum concentration is 0.5 μ gmL-1Avermectin handle 2 ages children
Worm for 24 hours after, the death rate and corrected mortality of 2 instar larvaes are respectively 45.77% and 45.77%；The death of 2 instar larvaes after 48h
Rate and corrected mortality are respectively 60.28% and 60.28%；The death rate of 2 instar larvaes and corrected mortality are respectively after 72h
73.08% and 71.37%.
3 long shoot trichoderma of table and avermectin 1:1 mixture (1 group of mixture) is to the toxic action of 2 instar larvaes
The comparison of 1 group of drug effect between single medicament of mixture：After long shoot trichoderma and avermectin mixture to 2 instar larvaes have compared with
Strong toxic action, effect are apparently higher than single trichoderma or single avermectin, significantly improve single trichoderma or single Ah
Tie up the toxic effect of 2 instar larvae of rhzomorph pair.As can be seen from Table 1, the death rate of 2 instar larvaes is after single trichoderma processing 72h
81.03%, corrected mortality 79.22%；0.5 μ gmL known to table 2-12 instar larvaes are dead after single avermectin processing 72
Rate is 73.08%, corrected mortality 71.37%；A concentration of 0.5 μ gmL of avermectin known to table 3-1Mixture handle 72h
The death rate of 2 instar larvaes is 96.30% afterwards, corrected mortality 96.12%.The death rate of mixture and corrected mortality are significantly high
In single trichoderma and single avermectin, show long shoot trichoderma with it is notable to the toxic action of 2 instar larvaes after avermectin mixture
It improves.In addition as can be seen from Table 3,2 instar larvae of part starts death after mixture processing for 24 hours, and as avermectin concentration becomes
There were significant differences for change, a concentration of 0.1 μ gmL of avermectin-1The death rate and corrected mortality of 2 instar larvae of mixture pair be respectively
57.94% and 57.94%.A concentration of 0.2 μ gmL of avermectin-1The death rate and corrected mortality of 2 instar larvae of mixture pair point
It Wei 65.00% and 65.00%.A concentration of 0.3 μ gmL of avermectin-1The death rate of 2 instar larvae of mixture pair and correction are dead
Rate is respectively 73.89% and 73.89%.A concentration of 0.4 μ gmL of avermectin-1The death rate of 2 instar larvae of mixture pair and correction
The death rate is respectively 82.22% and 82.22%.A concentration of 0.5 μ gmL of avermectin-1The death rate of 2 instar larvae of mixture pair and
Corrected mortality is respectively 86.67% and 86.67%.By LC50Value is it is found that different 2 instar larvae of avermectin concentration mixture pair
Toxic action is proportionate with avermectin concentration, and the toxic action of pair 2 instar larvaes is most strong in 72h, and 48h takes second place, for 24 hours most
Difference.
4 long shoot trichoderma of table and avermectin 2:1 mixture (2 groups of mixture) is to the toxic action of 2 instar larvaes
5 long shoot trichoderma of table and avermectin 3:1 mixture (3 groups of mixture) is to the toxic action of 2 instar larvaes
As seen from the experiment, after the long shoot trichoderma spore bacterium solution of different proportion is mixed with avermectin, all to 2 instar larvaes
With stronger toxic action, show that long shoot trichoderma spore bacterium solution is known as good compatibility with Avermectin.According to table 3, long shoot
Trichoderma and avermectin 1:1 mixing, a concentration of 0.5 μ gmL of avermectin-1, after handling 72h, the death rate of 2 instar larvaes and school
The positive death rate is up to 96.30% and 96.12% respectively.According to table 4, long shoot trichoderma and avermectin 2:1 mixing, avermectin are dense
Degree is 0.5 μ gmL-1, after handling 72h, the death rate and corrected mortality of 2 instar larvaes are up to 96.11% and 95.92% respectively.
According to table 5, long shoot trichoderma and avermectin 3:1 mixing, a concentration of 0.5 μ gmL of avermectin-1, after handling 72h, 2 instar larvaes
The death rate and corrected mortality are respectively 96.13% and 95.94%.It follows that long shoot trichoderma spore bacterium solution and avermectin
Can be compatible, and when with a concentration of 0.5 μ gmL-1Avermectin mixing when, long shoot trichoderma spore bacterium solution and AVM hereinafter
Rhzomorph carries out 1:1、2:1、3:1 three kinds of different ratio mixing have similar eelworm-killing activity, are not significantly different.
Claims (8)
1. a kind of mematocide, which is characterized in that the preparation is by abamectin solution and long shoot trichoderma bacterium
(T.longibrachiatum) bacterium solution forms, and the volume ratio of wherein abamectin solution and long shoot trichoderma bacterium bacterium solution is 1:1-3.
2. a kind of mematocide as described in claim 1, which is characterized in that a concentration of 0.1- of the abamectin solution
0.5μg·mL-1, viable bacteria concentration is 2 × 10 in long shoot trichoderma bacterium (T.longibrachiatum) bacterium solution7cfu·mL-1。
3. a kind of mematocide as claimed in claim 2, which is characterized in that a concentration of 0.1 μ of the abamectin solution
g·mL-1。
4. a kind of mematocide as claimed in claim 2, which is characterized in that a concentration of 0.2 μ of the abamectin solution
g·mL-1。
5. a kind of mematocide as claimed in claim 2, which is characterized in that a concentration of 0.3 μ of the abamectin solution
g·mL-1。
6. a kind of mematocide as claimed in claim 2, which is characterized in that a concentration of 0.4 μ of the abamectin solution
g·mL-1。
7. a kind of mematocide as claimed in claim 2, which is characterized in that a concentration of 0.5 μ of the abamectin solution
g·mL-1。
8. any one mematocide as described in claim 1-7, which is characterized in that the nematode is Singularity SCAR mark of wheat spore
Capsule nematode.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810198104.6A CN108338184A (en) | 2018-03-12 | 2018-03-12 | A kind of mematocide |
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| CN201810198104.6A CN108338184A (en) | 2018-03-12 | 2018-03-12 | A kind of mematocide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074064A (en) * | 2019-05-24 | 2019-08-02 | 甘肃农业大学 | A kind of Observe and measure method of trichoderma T6 strains on plant pathogenic nematode effect |
Citations (5)
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|---|---|---|---|---|
| CN1440652A (en) * | 2003-04-04 | 2003-09-10 | 于建垒 | Granulated abamectin nematocide |
| CN103805540A (en) * | 2014-01-15 | 2014-05-21 | 河南农业大学 | Biocontrol strain S07 for preventing and controlling cereal cyst nematode, method for preparing biocontrol fungicide and application of biocontrol fungicide |
| CN105284907A (en) * | 2015-11-19 | 2016-02-03 | 佳木斯兴宇生物技术开发有限公司 | Nematode killing granule containing avermectin and trichoderma harzianum |
| CN107509734A (en) * | 2016-06-16 | 2017-12-26 | 深圳诺普信农化股份有限公司 | A kind of nematicidal composition |
| CN107691457A (en) * | 2016-08-08 | 2018-02-16 | 深圳诺普信农化股份有限公司 | Nematicidal composition |
-
2018
- 2018-03-12 CN CN201810198104.6A patent/CN108338184A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1440652A (en) * | 2003-04-04 | 2003-09-10 | 于建垒 | Granulated abamectin nematocide |
| CN103805540A (en) * | 2014-01-15 | 2014-05-21 | 河南农业大学 | Biocontrol strain S07 for preventing and controlling cereal cyst nematode, method for preparing biocontrol fungicide and application of biocontrol fungicide |
| CN105284907A (en) * | 2015-11-19 | 2016-02-03 | 佳木斯兴宇生物技术开发有限公司 | Nematode killing granule containing avermectin and trichoderma harzianum |
| CN107509734A (en) * | 2016-06-16 | 2017-12-26 | 深圳诺普信农化股份有限公司 | A kind of nematicidal composition |
| CN107691457A (en) * | 2016-08-08 | 2018-02-16 | 深圳诺普信农化股份有限公司 | Nematicidal composition |
Non-Patent Citations (4)
| Title |
|---|
| ZHANG S W ET AL,: "The parasitic and lethal effects of Trichoderma longibrachiatum against Heterodera avenae", 《BIOLOGICAL CONTROL》 * |
| 张树武 等，: ""长枝木霉对小麦禾谷孢囊线虫的致死作用"，", 《应用生态学报》 * |
| 慕立义主编，: "《植物化学保护研究方法》", 31 October 1994, 中国农业出版社 * |
| 郝瑞 等，: ""新型种衣剂防治小麦禾谷孢囊线虫病研究"，", 《植物保护》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074064A (en) * | 2019-05-24 | 2019-08-02 | 甘肃农业大学 | A kind of Observe and measure method of trichoderma T6 strains on plant pathogenic nematode effect |
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