CN105283550A - 生物样品的稳定化 - Google Patents

生物样品的稳定化 Download PDF

Info

Publication number
CN105283550A
CN105283550A CN201480025870.2A CN201480025870A CN105283550A CN 105283550 A CN105283550 A CN 105283550A CN 201480025870 A CN201480025870 A CN 201480025870A CN 105283550 A CN105283550 A CN 105283550A
Authority
CN
China
Prior art keywords
sample
cell
stabilization
nucleic acid
stabilizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201480025870.2A
Other languages
English (en)
Chinese (zh)
Inventor
拉尔夫·怀里希
托斯滕·沃斯
凯利·京特
乌伟·厄尔米勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CN105283550A publication Critical patent/CN105283550A/zh
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201480025870.2A 2013-03-18 2014-03-18 生物样品的稳定化 Withdrawn CN105283550A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361803107P 2013-03-18 2013-03-18
US61/803,107 2013-03-18
EP13160896.0 2013-03-25
EP13160896 2013-03-25
EP13180130.0 2013-08-12
EP13180130 2013-08-12
PCT/EP2014/000725 WO2014146781A1 (en) 2013-03-18 2014-03-18 Stabilisation of biological samples

Publications (1)

Publication Number Publication Date
CN105283550A true CN105283550A (zh) 2016-01-27

Family

ID=51579331

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480025870.2A Withdrawn CN105283550A (zh) 2013-03-18 2014-03-18 生物样品的稳定化

Country Status (5)

Country Link
US (2) US11525155B2 (https=)
EP (2) EP3447141B1 (https=)
JP (2) JP6381628B2 (https=)
CN (1) CN105283550A (https=)
WO (1) WO2014146781A1 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11021733B2 (en) 2011-09-26 2021-06-01 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
EP2761001B1 (en) 2011-09-26 2018-08-01 Qiagen GmbH Rapid method for isolating extracellular nucleic acids
CN103827303B (zh) 2011-09-26 2021-10-22 普瑞阿那利提克斯有限公司 细胞外核酸的稳定化和分离
JP6329072B2 (ja) 2011-09-26 2018-05-23 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 細胞外核酸の安定化および単離
CN104755628B (zh) 2012-09-25 2019-03-01 凯杰有限公司 生物样品的稳定化
JP6407248B2 (ja) 2013-03-18 2018-10-17 キアゲン ゲーエムベーハー 細胞外核酸の安定化および単離
JP6664332B2 (ja) 2014-03-18 2020-03-13 キアゲン ゲーエムベーハー 細胞外核酸の安定化および単離
EP3256849A4 (en) * 2015-02-09 2018-12-05 Abogen, Inc. Devices, solutions and methods for sample collection related applications, analysis and diagnosis
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
EP3377645B1 (en) 2015-11-20 2023-10-04 Qiagen GmbH Method of preparing sterilized compositions for stabilization of extracellular nucleic acids
ES2694285T3 (es) * 2016-02-11 2018-12-19 Sarstedt Ag & Co. Kg Dispositivo y procedimiento para el aislamiento de ácidos nucleicos de sangre entera
CA3025569A1 (en) 2016-05-27 2017-11-30 Norgen Biotek Corp. Preservation of cell-free nucleic acids in biological samples
US10870891B2 (en) 2017-01-05 2020-12-22 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
US12227808B2 (en) * 2017-12-19 2025-02-18 The Wistar Institute Of Anatomy And Biology Compostions and methods for diagnosing lung cancers using gene expression profiles
EP4397771A3 (en) 2019-01-04 2024-10-16 PreAnalytiX GmbH Urine stabilization
CN114450402A (zh) * 2019-09-24 2022-05-06 恰根有限公司 稳定化的含细胞体液样品的多模式分析
EP4477751A1 (en) 2023-06-16 2024-12-18 QIAGEN GmbH Body fluid stabilization and processing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004024958A1 (en) * 2002-09-16 2004-03-25 Corning Incorporated Nucleic-acid ink compositions for arraying onto a solid support
US20100184096A1 (en) * 2000-04-18 2010-07-22 Amgen Inc. Screening methods using sitosterolemia susceptibility gene (ssg) polypeptides
WO2010096323A1 (en) * 2009-02-18 2010-08-26 Streck, Inc. Preservation of cell-free nucleic acids
US20100255524A1 (en) * 2007-05-31 2010-10-07 Qiagen Gmbh Method for stabilising a biological sample
WO2011057184A1 (en) * 2009-11-09 2011-05-12 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample

Family Cites Families (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3389133A (en) 1966-08-10 1968-06-18 Schwarz Biores Inc Process for isolating and purifying soluble ribonucleic acid
US3903179A (en) 1973-10-15 1975-09-02 Gulf Research Development Co Method for preparing nitrocyclopropane from 3-chloro-1-nitropropane
JPS6083581A (ja) * 1983-10-13 1985-05-11 Hideaki Yamada シユ−ドモナス属細菌の培養法
US4938961A (en) 1989-04-28 1990-07-03 Geoffrey Collins Organ preservation solution containing pokyethylene gycol and method of performing cardioplegia
US5459073A (en) 1991-05-08 1995-10-17 Streck Laboratories, Inc. Method and composition for preserving antigens and process for utilizing cytological material produced by same
US5460797A (en) 1991-05-08 1995-10-24 Streck Laboratories, Inc. Method for fixing tissues and cells for analysis using oxazolidine compounds
GB9215002D0 (en) * 1992-07-15 1992-08-26 Univ Singapore Method of fixing biological tissue
US6180778B1 (en) 1994-02-11 2001-01-30 Qiagen Gmbh Process for the separation of double-stranded/single-stranded nucleic acid structures
EP0696597B1 (de) 1994-08-11 2008-03-26 Sanofi-Aventis Deutschland GmbH Monoklonale Antikörper zur selektiven immunologischen Bestimmung von hochmolekularen, intakten Lamininformen in Körperflüssigkeiten
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5817470A (en) 1995-03-10 1998-10-06 Sociedad Biotecnologica Collico Limitada Immobilization of antigens to solid support by the mussel adhesive polyphenolic protein and the method for use therein
US6673364B1 (en) 1995-06-07 2004-01-06 The University Of British Columbia Liposome having an exchangeable component
US20050158699A1 (en) 1995-06-07 2005-07-21 Kadkade Prakash G. Cryopreservation of plant cells
ATE284891T1 (de) 1996-02-14 2005-01-15 Biomerieux Bv Isolierung von einzelsträngigen nukleinsäuren
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
ATE471150T1 (de) 1996-03-26 2010-07-15 Kopreski Michael S Methoden aus plasma oder serum extrahierte extrazelluraere rna zur diagnoseüberwachung oder evaluation von krebs verwenden
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
WO1998029126A1 (en) * 1996-12-27 1998-07-09 Sherwood Medical Company Anticoagulant composition
WO1998041651A1 (en) 1997-03-18 1998-09-24 Hsc Research & Development Limited Partnership Method for preparing chromatin
DE19726362A1 (de) 1997-06-21 1998-12-24 Schaeffler Waelzlager Ohg Vorrichtung zum Verändern der Steuerzeiten von Gaswechselventilen einer Brennkraftmaschine
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
ATE371022T1 (de) 1998-06-17 2007-09-15 Ge Healthcare Bio Sciences Fy7 polymerase
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US6407107B1 (en) 1999-05-28 2002-06-18 The Board Of Trustees Of The Unversity Of Arkansas Derivatives of butyric acid and uses thereof
US6379930B1 (en) 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails
US6492103B1 (en) 2000-01-31 2002-12-10 Organ Recovery Systems, Inc. System for organ and tissue preservation and hypothermic blood substitution
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
KR20030011279A (ko) 2000-03-23 2003-02-07 클리어런트, 인코포레이티드 생물학적 성분의 살균방법
DE10031236A1 (de) 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
ES2322841T3 (es) 2001-02-16 2009-06-30 Promega Corporation Aislamiento y purificacion magnetica de acidos nucleicos.
EP1425294B2 (en) 2001-08-23 2012-12-12 Veridex, LLC Analysis of circulating tumor cells, fragments, and debris
US20030064000A1 (en) 2001-09-24 2003-04-03 Wilson Burgess Methods of sterilizing biological mixtures using stabilizer mixtures
CA2465961A1 (en) 2001-11-06 2003-05-15 Cortex Biochem, Inc. Isolation and purification of nucleic acids
US6954128B2 (en) 2001-11-30 2005-10-11 The Regents Of The University Of California High performance hybrid magnetic structure for biotechnology applications
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
AU2002367863A1 (en) 2002-04-10 2003-10-27 Clearant, Inc. Methods for sterilizing biological materials
EP1516181A2 (en) 2002-05-07 2005-03-23 Becton Dickinson and Company Container for taking samples and in particular blood samples
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20060212020A1 (en) * 2002-10-10 2006-09-21 Lynne Rainen Sample collection system with caspase inhibitor
KR20050084082A (ko) 2002-12-02 2005-08-26 도요 고한 가부시키가이샤 폴리뉴클레오티드를 고체 지지체상에 고정화하는 방법,상기 방법에 의해 고정화되는 고체 지지체, 고체지지체로부터 표적 폴리뉴클레오티드를 검출하는 방법, 및폴리뉴클레오티드의 고정화 용액
US20040167165A1 (en) 2003-01-16 2004-08-26 Geetha Shankar Methods of treating conditions associated with an Edg-7 receptor
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US8758974B2 (en) 2003-03-27 2014-06-24 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Photoresist-free micropatterning on polymer surfaces
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
EP1510577A1 (en) 2003-08-29 2005-03-02 Qiagen GmbH Method for magnetic bead isolation of nucleic acids
EP1678121A4 (en) 2003-10-24 2007-07-25 Exelixis Inc MODULATORS OF TAO KINASES AND METHODS OF USE
US7270799B2 (en) 2004-01-15 2007-09-18 Nst Neurosurvival Technologies Ltd. Perturbed membrane-binding compounds and methods of using the same
JP4880484B2 (ja) 2004-02-20 2012-02-22 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 唾液ｍＲＮＡプロファイリング、バイオマーカーならびに関連した方法およびキット
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP1758937B1 (en) 2004-05-24 2009-09-02 Genvault Corporation Stable protein storage and stable nucleic acid storage in recoverable form
EP1773348A4 (en) 2004-07-12 2009-05-20 Idun Pharmaceuticals Inc TETRA PEPTIDE ANALOGS
US7727718B2 (en) 2005-01-04 2010-06-01 Molecular Research Center, Inc. Reagents for storage and preparation of samples for DNA analysis
WO2006085104A1 (en) 2005-02-11 2006-08-17 Invitrogen Dynal As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
FR2883003B1 (fr) 2005-03-14 2008-04-11 Aldivia Sa Nouveaux antioxydants a base d'especes d'anacardiacees, plus particulierement de sclerocarya birrea, leurs procedes d'obtention et leurs applications
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
AU2011253662A1 (en) 2005-11-30 2011-12-22 Massachusetts Institute Of Technology Pathogen-detecting cell preservation systems
EP1804045B1 (de) 2005-12-30 2014-03-12 QIAGEN GmbH Ein Verfahren und ein Kit zur Behandlung einer biologischen Probe
US20090029340A1 (en) 2006-01-04 2009-01-29 Do-Coop Technologies Ltd. Cryoprotective Compositions and Methods of Using Same
JP5459823B2 (ja) 2006-01-20 2014-04-02 リニューロン インコーポレイテッド 膵臓細胞培養における膵臓前駆細胞の細胞増殖促進方法
US20070190535A1 (en) 2006-02-13 2007-08-16 Hall Gerald E Jr Size fractionation of nucleic acid samples
CA2550133A1 (en) 2006-02-16 2007-08-16 The Governors Of The University Of Alberta Temperature regulated gene expression
JP5274781B2 (ja) 2007-03-22 2013-08-28 昭和電工株式会社 Ｒ−ｔ−ｂ系合金及びｒ−ｔ−ｂ系合金の製造方法、ｒ−ｔ−ｂ系希土類永久磁石用微粉、ｒ−ｔ−ｂ系希土類永久磁石
US7828887B2 (en) 2007-04-23 2010-11-09 Hewlett-Packard Development Company, L.P. Dye-based ink formulations
US7960098B2 (en) 2007-07-12 2011-06-14 Warsaw Orthoperic, Inc. Methods and compositions for the preservation of cells and tissues
US7892724B2 (en) 2007-07-12 2011-02-22 Warsaw Orthopedic, Inc Method for enhancing the viability of mammalian cells, tissues and organs using a solution comprising two low molecular weight PEGs
EP2027922A1 (de) 2007-08-02 2009-02-25 Qiagen GmbH Verfahren und Vorrichtung für das Fixieren/Stabilisieren einer Probe
WO2009042728A1 (en) * 2007-09-24 2009-04-02 Allelogic Biosciences Corporation Detection and /or quantification of nucleic acids
RU2496482C2 (ru) 2008-03-05 2013-10-27 Бакстер Интернэшнл Инк. Композиции и способы для доставки лекарственных средств
WO2010065420A2 (en) 2008-12-03 2010-06-10 Integrated Nano-Technologies, Inc. Universal biological sample processing
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
US20100280233A1 (en) 2009-05-04 2010-11-04 Connolly Dennis M Method for sample preparation
US20150056604A1 (en) 2009-06-15 2015-02-26 Biovec Transfusion, Llc Compositions and methods for preserving platelet function
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
TWI407994B (zh) 2009-10-22 2013-09-11 Ind Tech Res Inst 分離核酸的方法、試劑及套組
US8986609B2 (en) 2009-10-29 2015-03-24 Ascendis Pharma A/S Sterilization of biodegradable hydrogels
HUE052213T2 (hu) 2009-11-06 2021-04-28 Univ Leland Stanford Junior Grafitkilökõdés nem invazív diagnosztizálása szervátültetett betegekben
JP5773347B2 (ja) 2009-11-27 2015-09-02 国立大学法人 千葉大学 血液細胞の凍結保存剤
US8093427B2 (en) 2010-06-09 2012-01-10 National Tsing Hua University Construction and screening of solution-phase derived library of fenbufen and ethacrynic acid
US20130095473A1 (en) 2010-06-14 2013-04-18 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
FR2964567B1 (fr) 2010-09-14 2013-03-15 Lvmh Rech Extrait d'orchidee
EP3372230B1 (en) 2011-05-03 2020-08-12 Agios Pharmaceuticals, Inc. Pyruvate kinase activators for use for increasing lifetime of the red blood cells and treating anemia
US9664702B2 (en) * 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
JP6329072B2 (ja) * 2011-09-26 2018-05-23 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 細胞外核酸の安定化および単離
EP2761001B1 (en) 2011-09-26 2018-08-01 Qiagen GmbH Rapid method for isolating extracellular nucleic acids
CN103827303B (zh) * 2011-09-26 2021-10-22 普瑞阿那利提克斯有限公司 细胞外核酸的稳定化和分离
US11021733B2 (en) * 2011-09-26 2021-06-01 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
EP2760999A1 (en) 2011-09-26 2014-08-06 Qiagen GmbH Methods for separating nucleic acids by size
EP3663408B1 (en) 2011-10-11 2024-05-01 QIAGEN GmbH Sample processing method and sample processing cartridge
TWI719277B (zh) 2011-11-14 2021-02-21 美商安斯泰來再生醫藥協會 人類rpe細胞之醫藥組合物及其用途
US9546358B2 (en) 2012-06-04 2017-01-17 New England Biolabs, Inc. Compositions and methods for reducing background DNA amplification
CN104755628B (zh) * 2012-09-25 2019-03-01 凯杰有限公司 生物样品的稳定化
WO2014055936A1 (en) 2012-10-04 2014-04-10 Integenx Inc. Preservation of biological materials in non-aqueous fluid media
GB201303666D0 (en) 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
JP6407248B2 (ja) 2013-03-18 2018-10-17 キアゲン ゲーエムベーハー 細胞外核酸の安定化および単離
JP6664332B2 (ja) 2014-03-18 2020-03-13 キアゲン ゲーエムベーハー 細胞外核酸の安定化および単離
EP3698628A1 (en) 2014-08-07 2020-08-26 The General Hospital Corporation Stabilization of whole blood samples

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100184096A1 (en) * 2000-04-18 2010-07-22 Amgen Inc. Screening methods using sitosterolemia susceptibility gene (ssg) polypeptides
WO2004024958A1 (en) * 2002-09-16 2004-03-25 Corning Incorporated Nucleic-acid ink compositions for arraying onto a solid support
US20100255524A1 (en) * 2007-05-31 2010-10-07 Qiagen Gmbh Method for stabilising a biological sample
WO2010096323A1 (en) * 2009-02-18 2010-08-26 Streck, Inc. Preservation of cell-free nucleic acids
WO2011057184A1 (en) * 2009-11-09 2011-05-12 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CASERTA ET AL.: "Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties", 《APOPTOSIS》 *
SRINIVASAN RANGARAJAN ET AL.: "Extracellular nuclease from Rhizopus stolonifer: purification and characteristics of - single strand preferential - deoxyribonuclease activity", 《BIOCHIMICA ET BIOPHYSICA ACTA》 *
周立社主编: "《分子生物检验技术》", 31 March 2012, 人民军医出版社 *

Also Published As

Publication number Publication date
EP2976424B1 (en) 2018-10-03
JP2016514464A (ja) 2016-05-23
EP3447141A1 (en) 2019-02-27
WO2014146781A1 (en) 2014-09-25
US11525155B2 (en) 2022-12-13
US20160348151A1 (en) 2016-12-01
JP6750178B2 (ja) 2020-09-02
JP6381628B2 (ja) 2018-08-29
EP3447141B1 (en) 2020-08-05
JP2018166519A (ja) 2018-11-01
EP2976424A1 (en) 2016-01-27
US20220364149A1 (en) 2022-11-17

Similar Documents

Publication Publication Date Title
JP6750178B2 (ja) 生物試料の安定化
AU2019200643B2 (en) Stabilization and isolation of extracellular nucleic acids
JP6608280B2 (ja) 生物学的試料の安定化
JP6407248B2 (ja) 細胞外核酸の安定化および単離
CN103827322B (zh) 细胞外核酸的稳定化和分离
JP7548904B2 (ja) 尿安定化
CN103827303A (zh) 细胞外核酸的稳定化和分离
JP2019136044A (ja) 細胞外核酸の安定化および単離

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication

Application publication date: 20160127