CN105272626A - Culture medium capable of improving content of amino acids of lucid ganoderma - Google Patents
Culture medium capable of improving content of amino acids of lucid ganoderma Download PDFInfo
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- CN105272626A CN105272626A CN201510734831.6A CN201510734831A CN105272626A CN 105272626 A CN105272626 A CN 105272626A CN 201510734831 A CN201510734831 A CN 201510734831A CN 105272626 A CN105272626 A CN 105272626A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
Abstract
The invention discloses a culture medium capable of improving the content of amino acids of lucid ganoderma. The culture medium is prepared from the following raw materials in parts by weight: 50 to 60 parts of soybean meal, 10 to 20 parts of cotton seed hulls, 10 to 15 parts of sawdust, 10 to 15 parts of needle mushroom leftover grains, 20 to 30 parts of walnut shells, 10 to 20 parts of almond shells, 20 to 30 parts of walnut branches, 10 to 20 parts of needle mushroom leftover immersing water, 2 to 3 parts of lime, 2 to 3 parts of brown sugar and 8 to 20 parts of water. Compared with the prior art, the culture medium provided by the invention has comprehensive nutrients and the growth of the lucid ganoderma is facilitated; the content of the amino acids of the lucid ganoderma can be improved, and the health and medical values of the lucid ganoderma are improved.
Description
Technical field
The present invention relates to field of edible fungus culture, be specifically related to a kind of cultivation base improving Amino Acid of Glossy ganoderma content.
Background technology
Glossy ganoderma, also known as Ganoderma Lucidum, Ganoderma, Ganoderma lucidum seu Japonicum, is a kind of rare Chinese medicine in China's medical treasure-house, is collection health care and the medicinal edible mushrooms for the whole body.
The middle and high warm type wood-decay fungi of Ganoderma, its artificial culture materials mostly are deciduous tree weed tree sawdust, cotton seed hulls etc. at present, but nowadays, " bacterium-Lin " contradiction, the price continuous rise of cotton seed hulls, cultivation cost is more and more higher, and therefore each production unit all attaches great importance to looking for cheap and good-quality alternative materials.
As everyone knows, in cultivation of glossy ganoderma technology, need to provide appropriate carbon source, nitrogenous source, phosphorus source, mineral substance, VITAMIN.Adopt fertilizer to provide carbon source, nitrogenous source, phosphorus source can pollute, impact recycling.
Containing abundant amino acid in glossy ganoderma, and, amino acid whose content and medicinal, health value is closely related.There is provided abundant nutrition by cultivation base, the aminoacids content improving glossy ganoderma is necessary.
Therefore, provide a kind of cheapness, environmental protection the cultivation base of improved Amino Acid of Glossy ganoderma content necessary.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of cultivation base improving Amino Acid of Glossy ganoderma content, utilize and be rich in amino acid whose discarded needle mushroom tankage, walnut shell, Pericarppium Armeniacae Amarum, branch of Semen Juglandis be added in cultivation base as cultivation base auxiliary material, for glossy ganoderma provides abundant amino acid, improve the aminoacids content of glossy ganoderma.
A kind of cultivation base improving Amino Acid of Glossy ganoderma content provided by the invention, comprises the raw material of following weight part:
Preferably, the cultivation base of described raising Amino Acid of Glossy ganoderma content, comprises the raw material of following weight part:
The preparation method of described needle mushroom tankage particle is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2-3 days, crushed after being dried becomes 2-3mm particle, obtains needle mushroom tankage particle.
The preparation method that described needle mushroom tankage soak water is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2-3 days, crushed after being dried becomes 2-3mm particle, adds the water of 20-30 times of weight, soaks 1-2 days, filters, and obtains needle mushroom tankage and soaks water.
After described walnut shell, Pericarppium Armeniacae Amarum, dregs of beans, cotton seed hulls, wood dust dry, be broken into the particle of particle diameter 2mm-3mm, for subsequent use.
The preparation method of described branch of Semen Juglandis is:
Dried by the branch of Semen Juglandis cut, be cut into 4-5cm section, lime powder, is deposited in 5-12 in the environment of air seasoning individual month, then is broken into the particle of particle diameter 2mm-3mm, for subsequent use.
The usage quantity of lime powder is: the branch of Semen Juglandis after every 1kg dries limes powder 5-10g.
The preparation method of the cultivation base of described raising Amino Acid of Glossy ganoderma content, comprises the following steps:
(1), by after expanded for wood chip 10-15 part, mixing dregs of beans 50-60 part, cotton seed hulls 10-20 part, after mixing, add needle mushroom tankage and soak water 5-10 part, water 4-10 part, stir, build heap fermentation;
(2), after fermentation, add needle mushroom tankage particle 10-15 part, walnut shell 20-30 part, Pericarppium Armeniacae Amarum 10-20 part, branch of Semen Juglandis 20-30 part, needle mushroom tankage soak water 5-10 part, water 4-10 part, build heap fermentation;
(3), fermentation after, add lime 2-3 part, brown sugar 2-3 part, build heap fermentation, the cultivation base of the Amino Acid of Glossy ganoderma content that is improved.
Build heap fermentation described in step (1), be specially:
Stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation; After material temperature reaches 60 DEG C, once, ferment 4-5 days in turning every day.
Build heap fermentation described in step (2), be specially: after building heap, control atmospheric moisture 60-70%, keep ventilating, fermentation 3-4 days, turning every day once.
Build heap fermentation described in step (2), be specially: control atmospheric moisture 65-75%, keep ventilating, fermentation 3-4 days, turning every day once.
In the present invention, in cultivation base, add dregs of beans, dregs of beans contains protein, amino acid and abundant phosphoric, and the growth for glossy ganoderma provides abundant nitrogenous source, phosphorus source and amino acid.The add-on of cotton seed hulls and wood chip is according to the required carbon source rational proportion of glossy ganoderma growth, design.With the addition of brown sugar, with cotton seed hulls and wood chip work in coordination with provide glossy ganoderma grow required for carbon source.Add needle mushroom tankage, not only avoid waste and pollute, and the growth that the amino acid be rich in needle mushroom tankage is glossy ganoderma provides abundant raw material.And the needle mushroom tankage added soak water and stir raw material as water source, are beneficial to the absorption of glossy ganoderma growth.It is full-natural nutritive liquid that needle mushroom tankage soak water, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, mix and stir to carry out banking up with culturing raw material and ferment, promote beneficial microorganism breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material.Add branch of Semen Juglandis, walnut shell, Pericarppium Armeniacae Amarum, the growth for glossy ganoderma provides the nutrient on basis, and, and needle mushroom tankage are worked in coordination with as glossy ganoderma provides amino acid starting material.
In preparation process, through three fermentations, after each fermentation, add different raw materials.Needle mushroom tankage particle, walnut shell, Pericarppium Armeniacae Amarum and branch of Semen Juglandis add when second time fermentation, and prevent fermentation time long, nutritive ingredient is destroyed, and is unfavorable for the absorption of glossy ganoderma.Add lime powder the other side 5-10 month after branch of Semen Juglandis dries, both degraded poisonous bacteriostatics, make again the macromolecular substance such as xylogen, Mierocrystalline cellulose be decomposed into be conducive to the simple material that Ganoderma lucidum mycelium absorbs, facilitate the growth of Ganoderma lucidum mycelium; In addition, when the 2nd time and the 3rd fermentation, moisturizing humidity and ventilation condition, to make the material of fermentation go mouldy, maintain the nutrient that it is the highest.
Compared with prior art, cultivation base comprehensive nutrition provided by the invention, is conducive to the growth of glossy ganoderma, and, the aminoacids content of glossy ganoderma can be improved, improve its health care and pharmaceutical use.
Embodiment
Embodiment 1
Improve a cultivation base for Amino Acid of Glossy ganoderma content, comprise the raw material of following weight part:
The preparation method of described needle mushroom tankage particle is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2 days, crushed after being dried becomes 2mm particle, obtains needle mushroom tankage particle.
The preparation method that described needle mushroom tankage soak water is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 3 days, crushed after being dried becomes 3mm particle, adds the water of 30 times of weight, soaks 2 days, filters, and obtains needle mushroom tankage and soaks water.
After described walnut shell, Pericarppium Armeniacae Amarum, dregs of beans, cotton seed hulls, wood dust dry, be broken into the particle of particle diameter 2-3mm, for subsequent use.
The preparation method of described branch of Semen Juglandis is:
Dried by the branch of Semen Juglandis cut, be cut into 4-5cm section, the branch of Semen Juglandis after every 1kg dries limes powder 5g, to be deposited in the environment of air seasoning 5 months, then to be broken into the particle of particle diameter 2mm-3mm, for subsequent use.
The preparation method of the cultivation base of described raising Amino Acid of Glossy ganoderma content, comprises the following steps:
(1), by after expanded for wood chip 10 parts, mixing dregs of beans 50 parts, cotton seed hulls 10 parts, after mixing, add needle mushroom tankage and soak 5 parts, water, 5 parts, water, stir, build heap fermentation, stockpile is become high 1.0mm, wide 2m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg, after carrying out heap, surrounding is patted, then on heap, insert some vertical pores with the wooden stick of diameter 10cm, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation; After material temperature reaches 60 DEG C, turning every day once, is fermented 4 days.
(2), fermentation after, add needle mushroom tankage particle 10 parts, walnut shell 20 parts, Pericarppium Armeniacae Amarum 10 parts, branch of Semen Juglandis 20 parts, needle mushroom tankage soak 5 parts, water, 5 parts, water, build heap fermentation; Control atmospheric moisture 60%, keep ventilating, ferment 3 days, turning every day once;
(3), fermentation after, add 2 parts, lime, 2 parts, brown sugar, build heap fermentation, control atmospheric moisture 75%, keep ventilate, ferment 3 days, turning every day once, the cultivation base of the Amino Acid of Glossy ganoderma content that is improved.
Embodiment 2
Improve a cultivation base for Amino Acid of Glossy ganoderma content, comprise the raw material of following weight part:
The preparation method of described needle mushroom tankage particle is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2 days, crushed after being dried becomes 2mm particle, obtains needle mushroom tankage particle.
The preparation method that described needle mushroom tankage soak water is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 3 days, crushed after being dried becomes 3mm particle, adds the water of 20 times of weight, soaks 2 days, filters, and obtains needle mushroom tankage and soaks water.
After described walnut shell, Pericarppium Armeniacae Amarum, dregs of beans, cotton seed hulls, wood dust dry, be broken into the particle of particle diameter 2-3mm, for subsequent use.
The preparation method of described branch of Semen Juglandis is:
Dried by the branch of Semen Juglandis cut, be cut into 4-5cm section, the branch of Semen Juglandis after every 1kg dries limes powder 6g, to be deposited in the environment of air seasoning 12 months, then to be broken into the particle of particle diameter 2mm-3mm, for subsequent use.
The preparation method of the cultivation base of described raising Amino Acid of Glossy ganoderma content, comprises the following steps:
(1), by after expanded for wood chip 11 parts, mixing dregs of beans 56 parts, cotton seed hulls 13 parts, after mixing, add needle mushroom tankage and soak 7 parts, water, 5 parts, water, stir, build heap fermentation, stockpile is become high 1.0mm, wide 2m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg, after carrying out heap, surrounding is patted, then on heap, insert some vertical pores with the wooden stick of diameter 10cm, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation; After material temperature reaches 60 DEG C, turning every day once, is fermented 5 days.
(2), fermentation after, add needle mushroom tankage particle 12 parts, walnut shell 22 parts, Pericarppium Armeniacae Amarum 18 parts, branch of Semen Juglandis 25 parts, needle mushroom tankage soak 7 parts, water, 5 parts, water, build heap fermentation; Control atmospheric moisture 60%, keep ventilating, ferment 4 days, turning every day once;
(3), fermentation after, add 2 parts, lime, 2 parts, brown sugar, build heap fermentation, control atmospheric moisture 70%, keep ventilate, ferment 3 days, turning every day once, the cultivation base of the Amino Acid of Glossy ganoderma content that is improved.
Embodiment 3
Improve a cultivation base for Amino Acid of Glossy ganoderma content, comprise the raw material of following weight part:
The preparation method of described needle mushroom tankage particle is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2 days, crushed after being dried becomes 2mm particle, obtains needle mushroom tankage particle.
The preparation method that described needle mushroom tankage soak water is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 3 days, crushed after being dried becomes 3mm particle, adds the water of 20 times of weight, soaks 2 days, filters, and obtains needle mushroom tankage and soaks water.
After described walnut shell, Pericarppium Armeniacae Amarum, dregs of beans, cotton seed hulls, wood dust dry, be broken into the particle of particle diameter 2-3mm, for subsequent use.
The preparation method of described branch of Semen Juglandis is:
Dried by the branch of Semen Juglandis cut, be cut into 4-5cm section, the branch of Semen Juglandis after every 1kg dries limes powder 8g, to be deposited in the environment of air seasoning 10 months, then to be broken into the particle of particle diameter 2mm-3mm, for subsequent use.
The preparation method of the cultivation base of described raising Amino Acid of Glossy ganoderma content, comprises the following steps:
(1), by after expanded for wood chip 15 parts, mixing dregs of beans 59 parts, cotton seed hulls 20 parts, after mixing, add needle mushroom tankage and soak 10 parts, water, 10 parts, water, stir, build heap fermentation, stockpile is become high 1.0mm, wide 2m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg, after carrying out heap, surrounding is patted, then on heap, insert some vertical pores with the wooden stick of diameter 10cm, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation; After material temperature reaches 60 DEG C, turning every day once, is fermented 4 days.
(2), fermentation after, add needle mushroom tankage particle 15 parts, walnut shell 28 parts, Pericarppium Armeniacae Amarum 20 parts, branch of Semen Juglandis 30 parts, needle mushroom tankage soak 10 parts, water, 10 parts, water, build heap fermentation; Control atmospheric moisture 60%, keep ventilating, ferment 4 days, turning every day once;
(3), fermentation after, add 2 parts, lime, 2 parts, brown sugar, build heap fermentation, control atmospheric moisture 70%, keep ventilate, ferment 3 days, turning every day once, the cultivation base of the Amino Acid of Glossy ganoderma content that is improved.
Embodiment 1-3 is cultivated the glossy ganoderma that the glossy ganoderma obtained and common cultivation obtain, carry out analysis of amino acids test, result is as following table 1
Unit mg/100mg
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Common cultivation | |
| Aspartic acid | 0.691 | 0.6812 | 0.6721 | 0.5748 |
| Serine | 0.2903 | 0.3012 | 0.2818 | 0.1735 |
| L-glutamic acid | 0.6546 | 0.6318 | 0.6489 | 0.4235 |
| L-Ala | 0.4356 | 0.4316 | 0.4275 | 0.3015 |
| Methionine(Met) | 0.2812 | 0.2911 | 0.2738 | 0.1332 |
| α-amino-isovaleric acid | 0.6813 | 0.6635 | 0.6712 | 0.4283 |
| Methionin | 0.4121 | 0.4028 | 0.3931 | 0.2223 |
| Isoleucine | 0.3912 | 0.3934 | 0.3812 | 0.2701 |
| Phenylalanine | 0.3716 | 0.3654 | 0.3623 | 0.2564 |
| Leucine | 0.5011 | 0.4912 | 0.4833 | 0.2813 |
| Threonine | 0.3566 | 0.3718 | 0.3517 | 0.2801 |
Test result shows, the cultivation base of the embodiment 1-3 that the application provides plants the Amino Acid of Glossy ganoderma content that the obtains glossy ganoderma higher than common cultivation.
Claims (6)
1. improve a cultivation base for Amino Acid of Glossy ganoderma content, it is characterized in that, described cultivation base comprises the raw material of following weight part:
2. the cultivation base of raising Amino Acid of Glossy ganoderma content according to claim 1, is characterized in that, the preparation method of described needle mushroom tankage particle is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2-3 days, crushed after being dried becomes 2-3mm particle, obtains needle mushroom tankage particle.
3. the cultivation base of raising Amino Acid of Glossy ganoderma content according to claim 1 and 2, is characterized in that, the preparation method that described needle mushroom tankage soak water is:
After needle mushroom is gathered, take out needle mushroom tankage, shine 2-3 days, crushed after being dried becomes 2-3mm particle, adds the water of 20-30 times of weight, soaks 1-2 days, filters, and obtains needle mushroom tankage and soaks water.
4. the cultivation base of raising Amino Acid of Glossy ganoderma content according to claim 1 and 2, is characterized in that, after described walnut shell, Pericarppium Armeniacae Amarum, dregs of beans, cotton seed hulls, wood dust dry, is broken into the particle of particle diameter 2mm-3mm, for subsequent use.
5. the cultivation base of raising Amino Acid of Glossy ganoderma content according to claim 1 and 2, is characterized in that, the preparation method of described branch of Semen Juglandis is:
Dried by the branch of Semen Juglandis cut, be cut into 4-5cm section, lime powder, is deposited in 5-12 in the environment of air seasoning individual month, then is broken into the particle of particle diameter 2mm-3mm, for subsequent use.
6. the cultivation base of raising Amino Acid of Glossy ganoderma content according to claim 5, is characterized in that, the usage quantity of lime powder is: the branch of Semen Juglandis after every 1kg dries limes powder 5-10g.
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| CN114027089A (en) * | 2021-12-02 | 2022-02-11 | 沈阳农业大学 | Method for improving flavor of edible mushroom and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112056145A (en) * | 2020-10-20 | 2020-12-11 | 玉林市益康菌业种植有限公司 | Cultivation method for enriching nutrients of lucid ganoderma |
| CN114027089A (en) * | 2021-12-02 | 2022-02-11 | 沈阳农业大学 | Method for improving flavor of edible mushroom and application thereof |
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