CN105237123A - High-effective inonotus obliquus culture medium being rich in mineral elements and preparation method thereof - Google Patents

Abstract

The invention discloses a high-effective inonotus obliquus culture medium being rich in mineral elements, which includes the following components, by weight, porous gelatin/hyaluronic acid three-dimensional porous composite micro-spheres, cellulose acetate porous micro-spheres, castor meal powder, soybean meal powder, fermented animal excrements, expanded magnesite powder, chitosan ionic liquid, [beta]-oligomerized acid, yeast, and an amino acid-chelated mineral fertilizer. In the invention, the chitosan ionic liquid is added to the culture medium for treating the expanded magnesite powder and the like to prepare the easy-to-absorb inorganic mineral composite powder, which provides abundant minerals to the inonotus obliquus, thereby increasing mineral enrichment quantity of the inonotus obliquus and meanwhile increasing enrichment of other nutrients in the inonotus obliquus. By means of the castor meal powder for producing the inonotus obliquus, the inonotus obliquus is rich in nutrition. Fermentation is carried out with the yeast supported on the three-dimensional porous composite micro-spheres, so that decomposition degree of a substrate is improved and biomass conversion efficiency is also increased, thereby achieving high-effective and -quality cultivation of the inonotus obliquus.

Description

Fine pore fungi efficient culture medium of birch of a kind of enrichment mineral element and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus field, fine pore fungi efficient culture medium of birch particularly relating to a kind of enrichment mineral element and preparation method thereof.

Background technology

The fine pore fungi of birch belongs to Aphyllophorales Hymenochaetales, is that a kind of medicine food has edible mushrooms concurrently; Mineral element is the important component part of humans and animals body, has important physiological function.The mineral element major part be enriched in edible mushrooms exists with organic, the mineral element good stability of organic, be easy to accumulating, wherein the mineral element of most of organic all combines with the material such as protein, fats, and this combination facilitates absorption and the utilization of mineral element; Have research surface, the amino acid in the fine pore fungi of birch of enrichment mineral element, crude fat, coarse ash, crude protein content all increase, so the fine pore fungi of the birch cultivating enrichment mineral element is significant.

The micropore of the microballoon of vesicular structure is covered with whole microballoon and gos deep into center by surface, time as carrier, have, adsorption site is many, the features such as large specific surface area and strong adsorptive power, can play and change by features such as object carrier solvability, releasabilities, through being often used as the carrier of slow release fertilizer; In the preparation process of the submerged fermentation efficient culture medium of edible fungus culturing, the decomposition of culture medium raw material and fermentation directly decide the quality of substratum, and then determine that cultivation uses the effect of gold; The control of relevant enzyme and zymophyte activity is key and the difficult point of preparation submerged fermentation efficient culture medium, do not have the report of microballoon as the control of relevant enzyme and zymophyte activity in substratum making processes of vesicular structure at present, but according to the large specific surface area of porous microsphere, be conducive to sticking in a large number of cell, being conducive to the transmission of nutritive substance and the discharge of metabolic substd and this character of intercellular interaction, should be the control that can be used to enzyme and zymophyte activity.

Summary of the invention

Order of the present invention, for the Efficient Cultivation demand of the fine pore fungi of high quality birch, provides fine pore fungi efficient culture medium of a kind of birch of enrichment mineral element and preparation method thereof.

The present invention is achieved by the following technical solutions:

A kind of fine pore fungi efficient culture medium of birch of enrichment mineral element, comprise following parts by weight of component: the three-dimensional porous complex microsphere 3-4 of porous gelatin/hyaluronic acid, cellulose acetate porous microsphere 2-3, Castor seed Meal powder 30-34, bean cake powder 33-35, fermentation animal excrement 30-32, high calcium bone meal 18-20, sweet sorghum stalk 18-20, Radix Astragali stalk 19-21, beet pulp 34-35, expansion magnesite stone flour 1-2, expansion ground dolomite 1.5-1.9, ultra micro iron ore ground-slag 1.8-2, chitosan ionic liquid 2-3, sodium selenate 0.2-0.3, humic acids 0.8-0.9, Starso 0.2-0.3, sodium tetraborate 0.1-0.2, amino acid chelate liquid 0.5-0.7, β-oligomerization acid 0.3-0.4, selenium enriched yeast 1-1.1, yeast 2-3, the fertile 1-2 of chelating amino acids mineral, cellulose complex enzyme 1-2, nicotinic acid 0.5-1, VITAMIN 0.08-1, glycine 0.8-1, inositol 0.6-0.7, sucrose 2-3, Wingdale 1.5-2, appropriate water.

A birch fine pore fungi efficient culture medium preparation method for enrichment mineral element, comprises the following steps:

(1) by the three-dimensional porous complex microsphere of porous gelatin/hyaluronic acid, half yeast, sucrose mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte obtaining three-dimensional porous complex microsphere absorption loading is for subsequent use; By cellulose acetate porous microsphere, expansion magnesite stone flour, expansion ground dolomite, ultra micro iron ore ground-slag, the mixing of chitosan ionic liquid, and adding gross weight 3-4 water heated and stirred 2-3h at 60-70 DEG C doubly, it is for subsequent use that spraying dry obtains the mineral composite powder easily absorbed;

(2) sweet sorghum stalk, Radix Astragali stalk are first ground into the section of 2-3cm, adopt Wingdale and 10-12 water doubly afterwards, after mixing and stirring, soak 3-5d, and add nicotinic acid neutralization, add cellulose complex enzyme afterwards, stir enzymolysis 2-3h, the stalk liquid that must activate enzymolysis processing is for subsequent use;

(3) by sodium selenate, humic acids, Starso, sodium tetraborate, amino acid chelate liquid, β-oligomerization acid, selenium enriched yeast, chelating amino acids mineral fertilizer, VITAMIN, glycine, inositol mixing, and add gross weight 2-3 water doubly, at room temperature be stirred to evenly, obtain nutritive medium for subsequent use;

(4) by the stalk liquid of activation enzymolysis processing, Castor seed Meal powder, bean cake powder, fermentation animal excrement, high calcium bone meal, beet pulp, the mineral composite powder easily absorbed, nutritive medium mixing, and add gross weight 3-5 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 7-8d at 22-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the three-dimensional porous complex microsphere absorption of access activation loads, anaerobic fermentation 7-8d at 19-20 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 30-35d at 20.5-21.5 DEG C, during the fermentation, every 2-3d stirs once, stirs 35-45min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 6-10d out, adjusting moisture is afterwards the 60-68% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-28h, to obtain final product.

Advantage of the present invention is:

The present invention adds chitosan ionic liquid process expansion magnesite stone flour in the medium, the inorganic mineral composite powder being easy to absorb prepared by expansion ground dolomite, ultra micro iron ore ground-slag, for the fine pore fungi of birch provides abundant mineral substance, simultaneously, coordinate organic mineral substance, improve the mineral substance enriching quantity of the fine pore fungi of birch, also improve the enrichment of other nutritive substance of the fine pore fungi of birch simultaneously; The β added-oligomerization acid has the growth of promotion hypha of edible fungus, shortens growth cycle, improves effect of fruiting body yield; Adopt Castor seed Meal powder, bean cake powder, fermentation animal excrement, high calcium bone meal, sweet sorghum stalk, Radix Astragali stalk, beet pulp nutritious for the fine pore fungi of birch through fermentation, adopt three-dimensional porous complex microsphere to adsorb the zymophyte loaded to ferment, improve the degree of decomposition of substrate, make substrate higher by the absorption rate of the fine pore fungi of birch, improve Wood Adhesives from Biomass efficiency, the high-effect high-quality cultivation of the fine pore fungi of birch can be realized.

Embodiment

A kind of fine pore fungi efficient culture medium of birch of enrichment mineral element, comprise following parts by weight of component: the three-dimensional porous complex microsphere 3 of porous gelatin/hyaluronic acid, cellulose acetate porous microsphere 2, Castor seed Meal powder 30, bean cake powder 33, fermentation animal excrement 30, high calcium bone meal 18, sweet sorghum stalk 18, Radix Astragali stalk 19, beet pulp 34, expansion magnesite stone flour 1, expansion ground dolomite 1.5, ultra micro iron ore ground-slag 1.8, chitosan ionic liquid 2, sodium selenate 0.2, humic acids 0.8, Starso 0.2, sodium tetraborate 0.1, amino acid chelate liquid 0.5, β oligomerization acid 0.3, selenium enriched yeast 1-1.1, yeast 2, chelating amino acids mineral fertilizer 1, cellulose complex enzyme 1, nicotinic acid 0.5, VITAMIN 0.08, glycine 0.8, inositol 0.6, sucrose 2, Wingdale 1.5, appropriate water.

A birch fine pore fungi efficient culture medium preparation method for enrichment mineral element, comprises the following steps:

(1) by the three-dimensional porous complex microsphere of porous gelatin/hyaluronic acid, half yeast, sucrose mixing, and add the water of gross weight 2 times, moderate-speed mixer 2h, the zymophyte obtaining three-dimensional porous complex microsphere absorption loading is for subsequent use; By cellulose acetate porous microsphere, expansion magnesite stone flour, expansion ground dolomite, ultra micro iron ore ground-slag, the mixing of chitosan ionic liquid, and add water heated and stirred 2h at 60 DEG C of gross weight 3 times, it is for subsequent use that spraying dry obtains the mineral composite powder easily absorbed;

(2) sweet sorghum stalk, Radix Astragali stalk are first ground into the section of 2cm, adopt the water of Wingdale and 10 times afterwards, after mixing and stirring, soak 3d, and add nicotinic acid neutralization, add cellulose complex enzyme afterwards, stir enzymolysis 2h, the stalk liquid that must activate enzymolysis processing is for subsequent use;

(3) by sodium selenate, humic acids, Starso, sodium tetraborate, amino acid chelate liquid, the acid of β oligomerization, selenium enriched yeast, chelating amino acids mineral fertilizer, VITAMIN, glycine, inositol mixing, and add the water of gross weight 2 times, at room temperature be stirred to evenly, obtain nutritive medium for subsequent use;

(4) by the stalk liquid of activation enzymolysis processing, Castor seed Meal powder, bean cake powder, fermentation animal excrement, high calcium bone meal, beet pulp, the mineral composite powder easily absorbed, nutritive medium mixing, and add the water mixing and stirring of gross weight 3 times, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 7d at 22 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the three-dimensional porous complex microsphere absorption of access activation loads, anaerobic fermentation 7d at 19 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 30d at 20.5 DEG C, during the fermentation, every 2d stirs once, stirs 35min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 6d out, adjusting moisture is afterwards 60% of gross weight, and adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22h, to obtain final product.

Claims (2)

1. the fine pore fungi efficient culture medium of the birch of an enrichment mineral element, it is characterized in that, comprise following parts by weight of component: the three-dimensional porous complex microsphere 3-4 of porous gelatin/hyaluronic acid, cellulose acetate porous microsphere 2-3, Castor seed Meal powder 30-34, bean cake powder 33-35, fermentation animal excrement 30-32, high calcium bone meal 18-20, sweet sorghum stalk 18-20, Radix Astragali stalk 19-21, beet pulp 34-35, expansion magnesite stone flour 1-2, expansion ground dolomite 1.5-1.9, ultra micro iron ore ground-slag 1.8-2, chitosan ionic liquid 2-3, sodium selenate 0.2-0.3, humic acids 0.8-0.9, Starso 0.2-0.3, sodium tetraborate 0.1-0.2, amino acid chelate liquid 0.5-0.7, β-oligomerization acid 0.3-0.4, selenium enriched yeast 1-1.1, yeast 2-3, the fertile 1-2 of chelating amino acids mineral, cellulose complex enzyme 1-2, nicotinic acid 0.5-1, VITAMIN 0.08-1, glycine 0.8-1, inositol 0.6-0.7, sucrose 2-3, Wingdale 1.5-2, appropriate water.

2. a birch fine pore fungi efficient culture medium preparation method for enrichment mineral element, is characterized in that, comprise the following steps:

(1) by the three-dimensional porous complex microsphere of porous gelatin/hyaluronic acid, half yeast, sucrose mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte obtaining three-dimensional porous complex microsphere absorption loading is for subsequent use; By cellulose acetate porous microsphere, expansion magnesite stone flour, expansion ground dolomite, ultra micro iron ore ground-slag, the mixing of chitosan ionic liquid, and adding gross weight 3-4 water heated and stirred 2-3h at 60-70 DEG C doubly, it is for subsequent use that spraying dry obtains the mineral composite powder easily absorbed;

(2) sweet sorghum stalk, Radix Astragali stalk are first ground into the section of 2-3cm, adopt Wingdale and 10-12 water doubly afterwards, after mixing and stirring, soak 3-5d, and add nicotinic acid neutralization, add cellulose complex enzyme afterwards, stir enzymolysis 2-3h, the stalk liquid that must activate enzymolysis processing is for subsequent use;

(3) by sodium selenate, humic acids, Starso, sodium tetraborate, amino acid chelate liquid, β-oligomerization acid, selenium enriched yeast, chelating amino acids mineral fertilizer, VITAMIN, glycine, inositol mixing, and add gross weight 2-3 water doubly, at room temperature be stirred to evenly, obtain nutritive medium for subsequent use;

(4) by the stalk liquid of activation enzymolysis processing, Castor seed Meal powder, bean cake powder, fermentation animal excrement, high calcium bone meal, beet pulp, the mineral composite powder easily absorbed, nutritive medium mixing, and add gross weight 3-5 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 7-8d at 22-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the three-dimensional porous complex microsphere absorption of access activation loads, anaerobic fermentation 7-8d at 19-20 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 30-35d at 20.5-21.5 DEG C, during the fermentation, every 2-3d stirs once, stirs 35-45min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 6-10d out, adjusting moisture is afterwards the 60-68% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-28h, to obtain final product.