CN109644778A - A kind of edible fungus liquid fermentation medium and preparation method thereof - Google Patents
A kind of edible fungus liquid fermentation medium and preparation method thereof Download PDFInfo
- Publication number
- CN109644778A CN109644778A CN201811611944.7A CN201811611944A CN109644778A CN 109644778 A CN109644778 A CN 109644778A CN 201811611944 A CN201811611944 A CN 201811611944A CN 109644778 A CN109644778 A CN 109644778A
- Authority
- CN
- China
- Prior art keywords
- parts
- minutes
- extract
- fermentation medium
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The present invention provides a kind of edible fungus liquid fermentation medium and preparation method thereof, is specifically related to technical field of bioengineering.The present invention is mixed with edible fungus liquid fermentation medium using wheat bran extract, potato extract, brown sugar, glucose, mulberry extract, Azolla imbricata and stone precipice tea as primary raw material, when Jia Shui is prepared into fluid matrix, dissolubility is good, nutritional ingredient can be well dissolved in water, the parent species accessed in this way can directly absorb nutritional ingredient from water, the growth and development and nutriment accumulation, the enrichment for nutriment after fruit-body formation for being conducive to parent species mycelia provide material base.Liquid fermentation medium prepared by the present invention can effectively facilitate mycelial growth, improve mycelium dry weight and diameter；The content that nutriment in the fructification harvested after cultivation can be improved simultaneously, improves the nutritive value of fructification.Preparation method of the invention is reliable and stable, parameter is easily controllable, is suitable for large-scale production.
Description
[technical field]
The present invention relates to technical field of bioengineering, and in particular to a kind of edible fungus liquid fermentation medium and its preparation side
Method.
[background technique]
Liquid fermentation technology is one of modern biotechnology, it refers in biochemical reactor, and natural imitation circle will eat medicine
With bacterium in growing process some microelements such as necessary carbohydrate, organic and inorganic compound containing nitrogen, inorganic salts
And other nutriments are dissolved in water as culture medium, access parent species after sterilizing, are passed through filtrated air and are stirred, are mentioned
Oxygen required for edible bacterium thallus respiratory metabolism, and suitable external condition is controlled, carry out the breeding of mycelia mass propgation
Process.It is known as liquid spawn using the strain that liquid fermentation technology breeds out, there is significant advantage relative to solid culture,
Its culture medium prepares that raw material sources are extensive, cheap, and mycelia is with short production cycle, cell age is neat, can extensive, anniversary metaplasia produce
Strain.
In liquid fermentation technology, the formula composition and preparation process of fluid nutrient medium are to influence the key factor of fermentation,
Can substance supply high-quality nutriment for mycelia in culture medium, have a major impact to mycelia upgrowth situation superiority and inferiority.Currently, to food
Gradually increased with the research of bacteria liquid fermentation medium, wherein China Patent Publication No. CN106148204A, a kind of entitled purple
The patent document of sesame liquid fermentation medium and preparation method thereof discloses a kind of using potato, corn flour, glucose, di(2-ethylhexyl)phosphate
The fluid nutrient medium of hydrogen potassium, magnesium sulfate and the preparation of jujube slag extracting solution, improves the speed of growth of G.japonicum mycelium, shortens production
Period, but in this method culture medium solubility it is not high, directly affect absorption of the mycelia to nutritional ingredient, make mycelial life
Length is subject to certain restrictions, and development is not healthy and strong enough, to affect the accumulation of nutriment in fructification, leads to nutrition content
It is low；China Patent Publication No. is CN106316526A, and a kind of grifola frondosus liquid fermentation medium of name and preparation method thereof is special
Sharp document also discloses a kind of using wheat bran, glucose, potassium dihydrogen phosphate, magnesium sulfate, corn flour, peptone, calcium chloride, dimension life
The fluid nutrient medium of plain B1 and corn stigma juice preparation, improve the speed of growth of maitake mushroom mycelia, shorten the production cycle, but
This method only focuses on mycelial excessive growth, lacks to the accumulation of its nutriment and payes attention to, keeps fructification nutrition content low.
Therefore, to obtain the edible mushroom mycelium that the speed of growth is fast, growing way is vigorous, development is healthy and strong, while to obtain nutrition
The high fructification of content of material, it is necessary to develop a kind of applied widely, practical edible fungus liquid fermentation medium.
[summary of the invention]
Goal of the invention of the invention is, seeks for hypha of edible fungus excessive growth, fructification in edible fungus liquid fermented and cultured
The low problem of content of material is supported, a kind of edible fungus liquid fermentation medium and preparation method thereof is provided.Liquid prepared by the present invention
Fermentation medium can effectively facilitate mycelial growth, compared with prior art, propose mycelium dry weight raising 50% and diameter
It is high by 30%；The content that nutriment in the fructification harvested after cultivation can be improved simultaneously, improves the nutritive value of fructification.This hair
Bright preparation method is reliable and stable, parameter is easily controllable, is suitable for large-scale production.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of edible fungus liquid fermentation medium, which is characterized in that the raw material including following weight part ratio: water 100-200
Part, 25-55 parts of wheat bran extract, 15-45 parts of potato extract, 5-45 parts of brown sugar, 5-25 parts of glucose, mulberry extract 3-15
Part, 1-10 parts of Azolla imbricata, tea 1-5 parts of stone precipice, 1-3 parts of potassium dihydrogen phosphate and 0.5-1.5 parts of magnesium sulfate.
Optimization, the mulberry extract the preparation method comprises the following steps: by weight, taking 5-15 parts of ramulus mori, being crushed, so
Afterwards plus after immersion 20-50 minutes of 25-65 parts of water, first time refluxing extraction is carried out, is then concentrated, keeps volume after concentration dense
The 1/5-1/2 of contracting front volume adds 30-50 parts of mixed solution of polyethylene glycol and n-butanol, carries out second of refluxing extraction,
Then filtrate is collected by filtration, is then concentrated and dried filtrate, obtains mulberry extract.
It advanced optimizes, the mass parts of polyethylene glycol and n-butanol in the mixed solution of the polyethylene glycol and n-butanol
Than for 1-5:37-57.
Further optimize, the temperature of the first time refluxing extraction is 100-120 DEG C, extraction time is 60-90 points
Clock；The temperature of second of refluxing extraction is 130-150 DEG C, extraction time is 80-120 minutes.
Further optimize again, the potato extract the preparation method comprises the following steps: by weight, take 8-18 parts of potato,
It is crushed, then plus 30-50 parts of water are boiled 50-80 minutes, are then filtered, and filter residue is collected, and then take filter residue, and muscardine is added
It 0.1-1 parts of pulvis, cultivates 20-48 hours at 18-32 DEG C, then plus 15-35 parts of water, is ultrasonically treated 75- at 50-70 DEG C
It 105 minutes, then filters, collects filtrate, ozone is then passed through in filtrate, it is dry to carry out concentration after 24-72 hours for closed standing
It is dry, obtain potato extract.
Further optimize again, the wheat bran extract the preparation method comprises the following steps: by weight, take 13-27 parts of wheat bran,
It is crushed, then Plus acidic aqueous solution 50-100 parts, insulation reaction 80-100 minutes at 50-60 DEG C, then continues to heat up
It to boiling, is kept for fluidized state 15-25 minutes, then cools down, then filter, collected filtrate, take filtrate to be concentrated and dried, obtain wheat bran
Extract.
Further optimize again, the acidic aqueous solution the preparation method comprises the following steps: by weight, take the mass fraction to be
70-90 parts of the aqueous hydrochloric acid solution of 0.05%-0.5%, be added dropwise hydrogen peroxide 0.5-5 parts, stir evenly to get.
A kind of preparation method of above-mentioned edible fungus liquid fermentation medium, the following steps are included:
A. by weight, stone precipice tea and Azolla imbricata are taken, is uniformly mixed, is then crushed, obtain mixture A, it is spare；
B. by weight, after taking brown sugar and glucose, heating to melt, insulated and stirred 10-30 minutes, it is subsequently added into step
Mixture A obtained in a continues insulated and stirred 15-25 minutes, and then plus water is heated to reflux 30-50 minutes, finally filters, and receives
Collect filtrate, takes filtrate to be concentrated and dried, obtain sugared mixture；
C. by weight, take wheat bran extract, potato extract, mulberry extract, sugared mixture, potassium dihydrogen phosphate and
Magnesium sulfate after crushing respectively, is uniformly mixed to get fermentation medium.
Optimization, the temperature of heating described in step b is 120-150 DEG C；The temperature being heated to reflux is 90-100 DEG C.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1. the present invention is with wheat bran extract, potato extract, brown sugar, glucose, mulberry extract, Azolla imbricata and stone precipice tea
Be mixed with edible fungus liquid fermentation medium for primary raw material, when Jia Shui is prepared into fluid matrix, dissolubility is good, nutrition at
Dividing can be well dissolved in water, and the parent species accessed in this way can directly absorb nutritional ingredient from water, be conducive to parent species mycelia
Growth and development and nutriment accumulation, the enrichment for nutriment after fruit-body formation provides material base.Present invention preparation
Liquid fermentation medium can effectively facilitate mycelial growth, compared with prior art, make mycelium dry weight improve 50% He
Diameter improves 30%；The content that nutriment in the fructification harvested after cultivation can be improved simultaneously, improves the nutriture value of fructification
Value.Preparation method of the invention is reliable and stable, parameter is easily controllable, is suitable for large-scale production.
2. using the polyhydroxy piperazine in the mixed solution of polyethylene glycol and n-butanol enrichment ramulus mori aqueous extract in the present invention
Pyridine Alkaloid ingredient, this Alkaloid can inhibit intramycelial glucuroide and the polysaccharide of itself are degraded into monosaccharide, promote
Into the accumulation of polysaccharide in mycelia, the content of polysaccharide in fructification is improved.Using polyethylene glycol and n-butanol mixed solution, to ramulus mori
It carries out repeating extraction, can use polyethylene glycol reduces Sangzhi cell wall tension, and promote n-butanol to incorporate in ramulus mori cell,
The alkaloid being not readily dissolved in water is extracted, alkaloid is enriched with.
3. the present invention decomposes starch in potato using muscardine pulvis and potato extract is prepared, can make in potato
Starch, fiber, which are converted to, can directly be eaten the small-molecule substance that starter kind is absorbed and utilized, and improve growth vigor and the battalion of mycelia
Support the enrichment of substance.Muscardine pulvis be muscardine powdered spore, can on potato germination and growth, decompose potato in shallow lake
Monosaccharide small molecule needed for powder, fiber are converted into thallus, becomes high-quality carbon source.After muscardine is used to decompose potato in the present invention,
It is passed through ozone to kill muscardine, dead thallus can also be eaten the suction of starter kind by ozone degradation at a variety of nutriments
It receives and utilizes.
4. wheat bran extract is prepared using the protein in acidic aqueous solution hydrolysis wheat bran in the present invention, can be by wheat bran
In protein degradation abundant at micromolecule polypeptide, amino acid etc. can directly be eaten the absorption of starter kind as high-quality nitrogen source
It utilizes, improves the enrichment of the growth vigor and nutriment of mycelia.It generallys use hydrochloric acid or protein is hydrolyzed in sulfuric acid, but
Such method time-consuming was up to for more than ten a hours, and the dosage of acid is larger, was easy enriching heavy metal, polluted culture medium.In the present invention
The dosage of acid is reduced using the method that hydrogen peroxide is added, and after hydrogen peroxide is added, when can greatly shorten the degradation of protein
Between.
5. when preparing edible fungus liquid fermentation medium, glucose, brown sugar, stone precipice tea and Azolla imbricata are heated by the present invention
Saccharogenesis mixture is prepared, the content of medium molecular saccharides, and the type of abundant amino acid can be increased, and increase mycelia
Necessary microelement is developed, not only makes mycelium flourishing healthy and strong, moreover it is possible to make the nutriment that can be enriched with high-content in fructification.
Firstly, making carbohydrate form water-soluble colloid aggregation, at this time the mine in brown sugar using the method for glucose and brown sugar heating coking
Substance is soluble in colloid to keep stable ionic valence condition, increases necessary microelement for culture medium.Secondly, plus
Enter Azolla imbricata and stone precipice tea continues to heat together, colloid can be made effectively to soften and permeate the cell wall of both raw materials, makes thin
Cell lysis, which is burst, to be split, thus the Huang by the proteolytic in the flavonoids and Azolla imbricata in the tea of stone precipice in colloid, in the tea of stone precipice
Letones have antioxidation, and the stability of matrix can be enhanced, nutritional ingredient in matrix is prevented to be oxidized and fail；And
Protein under high temperature action in Azolla imbricata can further decompose into amino acid, thus the amino in abundant wheat bran extract
Sour type.
6. the present invention by creative work many times, obtain by wheat bran extract, potato extract, brown sugar, glucose,
Mulberry extract, Azolla imbricata, stone precipice these raw materials of tea are arranged in pairs or groups in specific proportions, based on being extracted by water, and by a series of pure
Change step, can be prepared water-soluble good edible fungus fermented culture medium, the culture medium be prepared into liquid in use,
Stirring 5 minutes in boiling water just can be completely dissolved, and nutriment therein can also be completely dissolved and not generate precipitating in water, eat in this way
Just nutriment can be directly absorbed by way of hydrone is transported with starter kind, provide good growth for hyphal development growth
Environment.
[specific embodiment]
Below with reference to embodiment, the invention will be further described.
Embodiment 1
A kind of edible fungus liquid fermentative medium formula and the preparation method is as follows:
A. by weight, 1 part and 1 part of Azolla imbricata of stone precipice tea is taken, is uniformly mixed, is then crushed, obtain mixture A, it is spare；
B. by weight, 5 parts and 5 parts of glucose of brown sugar are taken, after being warming up to 120 DEG C of thawings, insulated and stirred 10 minutes, is connect
Mixture A obtained in addition step a, then plus 100 parts of water continue insulated and stirred 15 minutes, be heated to reflux at 90 DEG C
It 30 minutes, finally filters, collects filtrate, take filtrate to be concentrated and dried, obtain sugared mixture；
C. by weight, 25 parts of wheat bran extract, 15 parts of potato extract, 3 parts of mulberry extract, above-mentioned sugar mixing are taken
Object, 1 part of potassium dihydrogen phosphate and 0.5 part of magnesium sulfate after crushing respectively, are uniformly mixed to get fermentation medium.
Wherein, mulberry extract the preparation method comprises the following steps: by weight, take 5 parts of ramulus mori, crushed, then plus water 25
Part impregnate after twenty minutes, carry out first time refluxing extraction, setting Extracting temperature be 100 DEG C, extraction time is 60 minutes, then into
Row concentration makes 1/5 of volume condensate precursor product after being concentrated, adds 30 parts of mixed solution of polyethylene glycol and n-butanol, into
Second of refluxing extraction of row, setting Extracting temperature is 130 DEG C, extraction time is 80 minutes, and filtrate is then collected by filtration, and then will
Filtrate is concentrated and dried, and obtains mulberry extract；The quality of polyethylene glycol and n-butanol in the mixed solution of polyethylene glycol and n-butanol
Part is than being 1:37.
Potato extract the preparation method comprises the following steps: by weight, taking 8 parts of potato, being crushed, then plus 30 parts of water are boiled
It 50 minutes, then filters, collects filter residue, then take filter residue, be added 0.1 part of muscardine pulvis, cultivate 20 hours, connect at 18 DEG C
Plus 15 parts of water, be ultrasonically treated 75 minutes at 50 DEG C, then filters, collection filtrate, ozone is then passed through in filtrate, it is closed
After standing 24 hours, it is concentrated and dried, obtains potato extract.
Wheat bran extract the preparation method comprises the following steps: by weight, take 13 parts of wheat bran, crushed, it is then Plus acidic water-soluble
50 parts of liquid, insulation reaction 80 minutes at 50 DEG C then continue to be warming up to boiling, are kept for fluidized state 15 minutes, then cooled down,
Then it filters, collects filtrate, take filtrate to be concentrated and dried, obtain wheat bran extract；Acidic aqueous solution the preparation method comprises the following steps: by weight
Than, taking mass fraction is 70 parts of aqueous hydrochloric acid solution of 0.05%, is added dropwise 0.5 part of hydrogen peroxide, stir evenly to get.
Embodiment 2
A kind of edible fungus liquid fermentative medium formula and the preparation method is as follows:
A. by weight, 5 parts and 10 parts of Azolla imbricata of stone precipice tea are taken, is uniformly mixed, is then crushed, obtain mixture A, it is standby
With；
B. by weight, 45 parts and 25 parts of glucose of brown sugar are taken, after being warming up to 150 DEG C of thawings, insulated and stirred 30 minutes,
Then plus 200 parts of water it is subsequently added into mixture A obtained in step a, continues insulated and stirred 25 minutes, heated back at 100 DEG C
Stream 50 minutes, is finally filtered, and is collected filtrate, is taken filtrate to be concentrated and dried, obtain sugared mixture；
C. by weight, take 55 parts of wheat bran extract, 45 parts of potato extract, 15 parts of mulberry extract, above-mentioned sugar mixed
Conjunction object, 3 parts of potassium dihydrogen phosphate and 1.5 parts of magnesium sulfate after crushing respectively, are uniformly mixed to get fermentation medium.
Wherein, mulberry extract the preparation method comprises the following steps: by weight, take 15 parts of ramulus mori, crushed, then plus water 65
Part impregnate 50 minutes after, carry out first time refluxing extraction, setting Extracting temperature be 120 DEG C, extraction time is 90 minutes, then into
Row concentration makes 1/2 of volume condensate precursor product after being concentrated, adds 50 parts of mixed solution of polyethylene glycol and n-butanol, into
Second of refluxing extraction of row, setting Extracting temperature is 150 DEG C, extraction time is 120 minutes, filtrate is then collected by filtration, then
Filtrate is concentrated and dried, mulberry extract is obtained；The matter of polyethylene glycol and n-butanol in the mixed solution of polyethylene glycol and n-butanol
Amount part is compared for 5:57.
Potato extract the preparation method comprises the following steps: by weight, taking 18 parts of potato, being crushed, then plus 50 parts of water are boiled
Boiling 80 minutes, is then filtered, and collects filter residue, then takes filter residue, is added 1 part of muscardine pulvis, is cultivated 48 hours, connect at 32 DEG C
Plus 35 parts of water, be ultrasonically treated 105 minutes at 70 DEG C, then filters, collection filtrate, ozone is then passed through in filtrate, it is close
Standing is closed after 72 hours, is concentrated and dried, obtains potato extract.
Wheat bran extract the preparation method comprises the following steps: by weight, take 27 parts of wheat bran, crushed, it is then Plus acidic water-soluble
100 parts of liquid, insulation reaction 100 minutes at 60 DEG C then continue to be warming up to boiling, are kept for fluidized state 25 minutes, then dropped
Temperature then filters, and collects filtrate, takes filtrate to be concentrated and dried, obtain wheat bran extract；Acidic aqueous solution weighs the preparation method comprises the following steps: pressing
Measure part ratio, taking mass fraction is 90 parts of aqueous hydrochloric acid solution of 0.5%, is added dropwise 5 parts of hydrogen peroxide, stir evenly to get.
Embodiment 3
A kind of edible fungus liquid fermentative medium formula and the preparation method is as follows:
A. by weight, 2 parts and 3 parts of Azolla imbricata of stone precipice tea are taken, is uniformly mixed, is then crushed, obtain mixture A, it is spare；
B. by weight, 10 parts and 10 parts of glucose of brown sugar are taken, after being warming up to 128 DEG C of thawings, insulated and stirred 15 minutes,
Then plus 130 parts of water it is subsequently added into mixture A obtained in step a, continues insulated and stirred 17 minutes, heated back at 92 DEG C
Stream 35 minutes, is finally filtered, and is collected filtrate, is taken filtrate to be concentrated and dried, obtain sugared mixture；
C. by weight, 30 parts of wheat bran extract, 20 parts of potato extract, 5 parts of mulberry extract, above-mentioned sugar mixing are taken
Object, 1.5 parts of potassium dihydrogen phosphate and 0.7 part of magnesium sulfate after crushing respectively, are uniformly mixed to get fermentation medium.
Wherein, mulberry extract the preparation method comprises the following steps: by weight, take 7 parts of ramulus mori, crushed, then plus water 35
Part impregnate 27 minutes after, carry out first time refluxing extraction, setting Extracting temperature be 105 DEG C, extraction time is 67 minutes, then into
Row concentration makes 3/10 of volume condensate precursor product after being concentrated, adds 35 parts of mixed solution of polyethylene glycol and n-butanol, into
Second of refluxing extraction of row, setting Extracting temperature is 135 DEG C, extraction time is 90 minutes, and filtrate is then collected by filtration, and then will
Filtrate is concentrated and dried, and obtains mulberry extract；The quality of polyethylene glycol and n-butanol in the mixed solution of polyethylene glycol and n-butanol
Part is than being 2:43.
Potato extract the preparation method comprises the following steps: by weight, taking 10 parts of potato, being crushed, then plus 35 parts of water are boiled
Boiling 57 minutes, is then filtered, and collects filter residue, then takes filter residue, is added 0.3 part of muscardine pulvis, is cultivated 27 hours at 21 DEG C,
Then plus 20 parts of water, it is ultrasonically treated 83 minutes at 55 DEG C, then filters, collects filtrate, ozone is then passed through in filtrate, it is close
Standing is closed after 36 hours, is concentrated and dried, obtains potato extract.
Wheat bran extract the preparation method comprises the following steps: by weight, take 16 parts of wheat bran, crushed, it is then Plus acidic water-soluble
63 parts of liquid, insulation reaction 85 minutes at 52 DEG C then continue to be warming up to boiling, are kept for fluidized state 18 minutes, then cooled down,
Then it filters, collects filtrate, take filtrate to be concentrated and dried, obtain wheat bran extract；Acidic aqueous solution the preparation method comprises the following steps: by weight
Than, taking mass fraction is 75 parts of aqueous hydrochloric acid solution of 0.15%, is added dropwise 1.6 parts of hydrogen peroxide, stir evenly to get.
Embodiment 4
A kind of edible fungus liquid fermentative medium formula and the preparation method is as follows:
A. by weight, 4 parts and 7 parts of Azolla imbricata of stone precipice tea are taken, is uniformly mixed, is then crushed, obtain mixture A, it is spare；
B. by weight, 40 parts and 20 parts of glucose of brown sugar are taken, after being warming up to 143 DEG C of thawings, insulated and stirred 25 minutes,
Then plus 150 parts of water it is subsequently added into mixture A obtained in step a, continues insulated and stirred 22 minutes, heated back at 98 DEG C
Stream 45 minutes, is finally filtered, and is collected filtrate, is taken filtrate to be concentrated and dried, obtain sugared mixture；
C. by weight, take 50 parts of wheat bran extract, 40 parts of potato extract, 10 parts of mulberry extract, above-mentioned sugar mixed
Conjunction object, 2.5 parts of potassium dihydrogen phosphate and 1.2 parts of magnesium sulfate after crushing respectively, are uniformly mixed to get fermentation medium.
Wherein, mulberry extract the preparation method comprises the following steps: by weight, take 13 parts of ramulus mori, crushed, then plus water 55
Part impregnate 42 minutes after, carry out first time refluxing extraction, setting Extracting temperature be 115 DEG C, extraction time is 82 minutes, then into
Row concentration makes 4/10 of volume condensate precursor product after being concentrated, adds 45 parts of mixed solution of polyethylene glycol and n-butanol, into
Second of refluxing extraction of row, setting Extracting temperature is 145 DEG C, extraction time is 110 minutes, filtrate is then collected by filtration, then
Filtrate is concentrated and dried, mulberry extract is obtained；The matter of polyethylene glycol and n-butanol in the mixed solution of polyethylene glycol and n-butanol
Amount part is compared for 4:53.
Potato extract the preparation method comprises the following steps: by weight, taking 15 parts of potato, being crushed, then plus 45 parts of water are boiled
Boiling 72 minutes, is then filtered, and collects filter residue, then takes filter residue, is added 0.7 part of muscardine pulvis, is cultivated 41 hours at 28 DEG C,
Then plus 30 parts of water, it is ultrasonically treated 97 minutes at 65 DEG C, then filters, collects filtrate, ozone is then passed through in filtrate, it is close
Standing is closed after 60 hours, is concentrated and dried, obtains potato extract.
Wheat bran extract the preparation method comprises the following steps: by weight, take 23 parts of wheat bran, crushed, it is then Plus acidic water-soluble
85 parts of liquid, insulation reaction 95 minutes at 57 DEG C then continue to be warming up to boiling, are kept for fluidized state 22 minutes, then cooled down,
Then it filters, collects filtrate, take filtrate to be concentrated and dried, obtain wheat bran extract；Acidic aqueous solution the preparation method comprises the following steps: by weight
Than, taking mass fraction is 85 parts of aqueous hydrochloric acid solution of 0.4%, is added dropwise 3.8 parts of hydrogen peroxide, stir evenly to get.
Embodiment 5
A kind of edible fungus liquid fermentative medium formula and the preparation method is as follows:
A. by weight, 3 parts and 6 parts of Azolla imbricata of stone precipice tea are taken, is uniformly mixed, is then crushed, obtain mixture A, it is spare；
B. by weight, 15 parts and 15 parts of glucose of brown sugar are taken, after being warming up to 135 DEG C of thawings, insulated and stirred 20 minutes,
Then plus 170 parts of water it is subsequently added into mixture A obtained in step a, continues insulated and stirred 20 minutes, heated back at 95 DEG C
Stream 40 minutes, is finally filtered, and is collected filtrate, is taken filtrate to be concentrated and dried, obtain sugared mixture；
C. by weight, 35 parts of wheat bran extract, 30 parts of potato extract, 8 parts of mulberry extract, above-mentioned sugar mixing are taken
Object, 2 parts of potassium dihydrogen phosphate and 1 part of magnesium sulfate after crushing respectively, are uniformly mixed to get fermentation medium.
Wherein, mulberry extract the preparation method comprises the following steps: by weight, take 10 parts of ramulus mori, crushed, then plus water 45
Part impregnate 35 minutes after, carry out first time refluxing extraction, setting Extracting temperature be 110 DEG C, extraction time is 75 minutes, then into
Row concentration makes 7/20 of volume condensate precursor product after being concentrated, adds 40 parts of mixed solution of polyethylene glycol and n-butanol, into
Second of refluxing extraction of row, setting Extracting temperature is 140 DEG C, extraction time is 100 minutes, filtrate is then collected by filtration, then
Filtrate is concentrated and dried, mulberry extract is obtained；The matter of polyethylene glycol and n-butanol in the mixed solution of polyethylene glycol and n-butanol
Amount part is compared for 3:47.
Potato extract the preparation method comprises the following steps: by weight, taking 13 parts of potato, being crushed, then plus 40 parts of water are boiled
Boiling 65 minutes, is then filtered, and collects filter residue, then takes filter residue, is added 0.5 part of muscardine pulvis, is cultivated 34 hours at 25 DEG C,
Then plus 25 parts of water, it is ultrasonically treated 90 minutes at 60 DEG C, then filters, collects filtrate, ozone is then passed through in filtrate, it is close
Standing is closed after 48 hours, is concentrated and dried, obtains potato extract.
Wheat bran extract the preparation method comprises the following steps: by weight, take 20 parts of wheat bran, crushed, it is then Plus acidic water-soluble
75 parts of liquid, insulation reaction 90 minutes at 55 DEG C then continue to be warming up to boiling, are kept for fluidized state 20 minutes, then cooled down,
Then it filters, collects filtrate, take filtrate to be concentrated and dried, obtain wheat bran extract；Acidic aqueous solution the preparation method comprises the following steps: by weight
Than, taking mass fraction is 80 parts of aqueous hydrochloric acid solution of 0.3%, is added dropwise 2.7 parts of hydrogen peroxide, stir evenly to get.
Embodiment 6
In addition to the mixed solution for replacing polyethylene glycol and n-butanol with n-butanol when mulberry extract preparation, remaining method is same
Embodiment 5.
Embodiment 7
In addition to the mass parts ratio of ethylene glycol and n-butanol is 0.7:57 when mulberry extract preparation, the same embodiment of remaining method
5。
Embodiment 8
In addition to the mass parts ratio of ethylene glycol and n-butanol is 6:36 when mulberry extract preparation, remaining method is the same as embodiment 5.
Embodiment 9
In addition to being added without muscardine pulvis when potato extract preparation, remaining method is the same as embodiment 5.
Embodiment 10
In addition to replacing acidic aqueous solution by 2% aqueous hydrochloric acid solution with mass fraction when the preparation of wheat bran extract, remaining side
Method is the same as embodiment 5.
Embodiment 11
Then plus water 170 after taking brown sugar and glucose that mixture A is added at normal temperature in step b, 20 minutes are stirred,
Part, it is heated to reflux at 95 DEG C 40 minutes, finally filters, collect filtrate, filtrate is taken to be concentrated and dried, be made outside sugar mixture,
Remaining method is the same as embodiment 5.
Embodiment 12
It is CN106148204A, a kind of entitled purple sesame liquid fermentation medium and preparation method thereof by China Patent Publication No.
Patent document in the method for embodiment 3 liquid fermentation medium is prepared.
The comparison of 13 implementation result of embodiment
1. experimental method
Water is added by solid-liquid ratio 3:11 in the culture medium of Example 1-11 preparation respectively, and heating for dissolving is prepared 11 parts
Then fluid nutrient medium carries out high pressure steam sterilization, choosing with a collection of lucid ganoderma stock culture, agaric parent species, mushroom parent species, connecing respectively
Kind then enters fermentor and carries out hypha fermentation culture on 11 parts of culture mediums after above-mentioned sterilizing, and obtaining includes three kinds of mycelium
Laboratory sample 1-11, take laboratory sample 1-11 be inoculated in generation material culture medium, bacterium germination and management of producing mushroom method routinely, respectively
Obtain three kinds of edible mushrooms under each laboratory sample.
Above-mentioned same a batch is connect into lucid ganoderma stock culture, agaric parent species, mushroom parent species and is inoculated in the culture medium prepared by embodiment 12
On, obtaining includes three kinds of mycelial laboratory samples 12, take laboratory sample 12 to be inoculated in generation material culture medium, bacterium germination routinely and
Management of producing mushroom method respectively obtains three kinds of edible mushrooms under the laboratory sample.
2. evaluation method
The mycelial dry weight of 2.1 statistical experiment sample 1-12 and diameter, detailed results are shown in Table 1, table 2, table 3.
The measuring method of mycelium dry weight are as follows: take fermentation liquid, filter to obtain mycelium with Buchner funnel, then dried in 60 DEG C
To constant weight, in terms of mycelium dry mass (g/100mL) in every 100mL fermentation liquid.
The measuring method of mycelium diameter are as follows: it takes fermentation liquid to be diluted with water 10 times, takes 10 mycelia bulk measurement length at random,
It is averaged.
The measurement of 2.2 fructification nutriments
The nutriment of three kinds of edible mushrooms in difference measurement experiment sample 1-12, wherein pressing Chinese Pharmacopoeia version first in 2015
The measuring method of the 188-189 pages of portion ganoderma lucidum lower polysaccharide, triterpene and sterol is measured ganoderma lucidum in laboratory sample 1-12；It presses
Method in GB/T15672-2009 edible mushroom in the measurement of total sugar content surveys agaric in laboratory sample 1-12 and mushroom
It is fixed；By the second method spectrophotometry in the measurement of GB5009.5-2016 national food safety standard Protein in Food to experiment
Agaric and mushroom are measured in sample 1-12.Detailed results are shown in Table 1, table 2, table 3.
3. evaluation result
1 ganoderma lucidum quality evaluation result of table
2 agaric quality evaluation result of table
3 mushroom quality evaluation result of table
4. experimental result
Find out from table 1, table 2, table 3, laboratory sample 1-5 is better than laboratory sample 6-12.
When laboratory sample 6 prepares culture medium, second of refluxing extraction, 7 He of laboratory sample are carried out to ramulus mori using n-butanol
When laboratory sample 8 prepares culture medium, using ethylene glycol and n-butanol mixed solution extraction ramulus mori beyond ratio range, San Zhejun
Alkaloid is not extracted well, the accumulation of mycelia nutriment could not be promoted, lead to over growth of hyphae, but also son is real
Body nutrition content is low.
Laboratory sample 9 is added without muscardine pulvis when preparing potato extract, prevents the starch in potato from being degraded into
The small molecule monosaccharide easily absorbed, hinders the absorption of mycelia, causes hyphal development not sturdy enough, also leads to nutrients in fructification
Matter lacks.
For laboratory sample 10 when preparing wheat bran extract, 2% aqueous hydrochloric acid solution replaces acidic aqueous solution, so that in wheat bran
Protein hydrolysis it is not complete enough, cannot be absorbed and utilized by mycelia, cause hyphal development not sturdy enough, but also fructification nutrition
Content of material reduces.
For laboratory sample 11 when preparing culture medium, brown sugar and glucose are without coking, so that mycelia amino acid and mineral
Matter incomplete absorption, development is not sturdy enough, also leads to the decline of fructification nutrition content.
Laboratory sample 12 is the fluid nutrient medium of conventional prior art preparation, and over growth of hyphae has its table, fructification in vain
Nutrition content is also extremely low.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (9)
1. a kind of edible fungus liquid fermentation medium, which is characterized in that the raw material including following weight part ratio: 100-200 parts of water,
25-55 parts of wheat bran extract, 15-45 parts of potato extract, 5-45 parts of brown sugar, 5-25 parts of glucose, 3-15 parts of mulberry extract,
1-10 parts of Azolla imbricata, tea 1-5 parts of stone precipice, 1-3 parts of potassium dihydrogen phosphate and 0.5-1.5 parts of magnesium sulfate.
2. according to benefit require 1 described in a kind of edible fungus liquid fermentation medium, which is characterized in that the system of the mulberry extract
Preparation Method are as follows: by weight, take 5-15 parts of ramulus mori, crushed, then plus after immersion 20-50 minutes of 25-65 parts of water, carry out
First time refluxing extraction, is then concentrated, and is made the 1/5-1/2 of volume condensate precursor product after concentration, is added polyethylene glycol
With 30-50 parts of mixed solution of n-butanol, carry out second of refluxing extraction, filtrate is then collected by filtration, then filtrate is concentrated
It is dry, obtain mulberry extract.
3. according to benefit require 2 described in a kind of edible fungus liquid fermentation medium, which is characterized in that the polyethylene glycol and positive fourth
The mass parts of polyethylene glycol and n-butanol ratio is 1-5:37-57 in the mixed solution of alcohol.
4. according to benefit require 2 described in a kind of edible fungus liquid fermentation medium, which is characterized in that the first time refluxing extraction
Temperature be 100-120 DEG C, extraction time is 60-90 minutes；The temperature of second of refluxing extraction is 130-150 DEG C, mentions
Taking the time is 80-120 minutes.
5. according to benefit require 1 described in a kind of edible fungus liquid fermentation medium, which is characterized in that the system of the potato extract
Preparation Method are as follows: by weight, take 8-18 parts of potato, crushed, then plus 30-50 parts of water are boiled 50-80 minutes, then mistake
Filter collects filter residue, then takes filter residue, is added 0.1-1 parts of muscardine pulvis, cultivates 20-48 hours at 18-32 DEG C, then plus
It 15-35 parts of water, is ultrasonically treated 75-105 minutes at 50-70 DEG C, then filters, collect filtrate, be then passed through in filtrate smelly
Oxygen, closed standing are concentrated and dried after 24-72 hours, obtain potato extract.
6. according to benefit require 1 described in a kind of edible fungus liquid fermentation medium, which is characterized in that the system of the wheat bran extract
Preparation Method are as follows: by weight, 13-27 parts of wheat bran are taken, is crushed, then Plus acidic aqueous solution 50-100 parts, at 50-60 DEG C
Lower insulation reaction 80-100 minutes then continues to be warming up to boiling, is kept for fluidized state 15-25 minutes, then cooled down, then mistake
Filter collects filtrate, takes filtrate to be concentrated and dried, obtain wheat bran extract.
7. according to benefit require 6 described in a kind of edible fungus liquid fermentation medium, which is characterized in that the system of the acidic aqueous solution
Preparation Method are as follows: by weight, taking mass fraction is 70-90 parts of aqueous hydrochloric acid solution of 0.05%-0.5%, and hydrogen peroxide is added dropwise
0.5-5 parts, stir evenly to get.
8. a kind of preparation method of the described in any item edible fungus liquid fermentation mediums of claim 1-7, which is characterized in that packet
Include following steps:
A. by weight, stone precipice tea and Azolla imbricata are taken, is uniformly mixed, is then crushed, obtain mixture A, it is spare；
B. by weight, after taking brown sugar and glucose, heating to melt, insulated and stirred 10-30 minutes, it is subsequently added into step a
Mixture A obtained continues insulated and stirred 15-25 minutes, and then plus water is heated to reflux 30-50 minutes, finally filters, and collects filter
Liquid takes filtrate to be concentrated and dried, and obtains sugared mixture；
C. by weight, wheat bran extract, potato extract, mulberry extract, sugared mixture, potassium dihydrogen phosphate and sulfuric acid are taken
Magnesium after crushing respectively, is uniformly mixed to get fermentation medium.
9. according to benefit require 8 described in a kind of preparation method of edible fungus liquid fermentation medium, which is characterized in that in step b
The temperature of the heating is 120-150 DEG C；The temperature being heated to reflux is 90-100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811611944.7A CN109644778A (en) | 2018-12-27 | 2018-12-27 | A kind of edible fungus liquid fermentation medium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811611944.7A CN109644778A (en) | 2018-12-27 | 2018-12-27 | A kind of edible fungus liquid fermentation medium and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109644778A true CN109644778A (en) | 2019-04-19 |
Family
ID=66117151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811611944.7A Pending CN109644778A (en) | 2018-12-27 | 2018-12-27 | A kind of edible fungus liquid fermentation medium and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109644778A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326487A (en) * | 2019-08-06 | 2019-10-15 | 程亚龙 | Composition and its application containing ramulus mori |
| CN112956372A (en) * | 2021-03-11 | 2021-06-15 | 齐齐哈尔大学 | Method for cultivating lucid ganoderma |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828668A (en) * | 2010-04-13 | 2010-09-15 | 山东轻工业学院 | Method for preparing dietary fiber by combining enzymatic alkaline method and wet ultra-micro pulverization |
| CN102875450A (en) * | 2012-10-25 | 2013-01-16 | 上海丝绸集团股份有限公司 | Technological method for extracting 1-deoxynojirimycin from mulberry leaf |
| CN103058721A (en) * | 2013-01-17 | 2013-04-24 | 上海博琛生物科技有限公司 | Method for planting organic strawberry by micro-ecological technology |
| CN105052536A (en) * | 2015-07-23 | 2015-11-18 | 广西仙草堂制药有限责任公司 | Method for cultivating Lingzhi bacterial strains via submerged fermentation |
| CN107691109A (en) * | 2017-11-28 | 2018-02-16 | 广西仙草堂制药有限责任公司 | A kind of cultural method of ecological ganoderma lucidum |
-
2018
- 2018-12-27 CN CN201811611944.7A patent/CN109644778A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828668A (en) * | 2010-04-13 | 2010-09-15 | 山东轻工业学院 | Method for preparing dietary fiber by combining enzymatic alkaline method and wet ultra-micro pulverization |
| CN102875450A (en) * | 2012-10-25 | 2013-01-16 | 上海丝绸集团股份有限公司 | Technological method for extracting 1-deoxynojirimycin from mulberry leaf |
| CN103058721A (en) * | 2013-01-17 | 2013-04-24 | 上海博琛生物科技有限公司 | Method for planting organic strawberry by micro-ecological technology |
| CN105052536A (en) * | 2015-07-23 | 2015-11-18 | 广西仙草堂制药有限责任公司 | Method for cultivating Lingzhi bacterial strains via submerged fermentation |
| CN107691109A (en) * | 2017-11-28 | 2018-02-16 | 广西仙草堂制药有限责任公司 | A kind of cultural method of ecological ganoderma lucidum |
Non-Patent Citations (3)
| Title |
|---|
| 张炳炽: "《微生物学简明教程》", 31 December 1988, 青岛出版社 * |
| 王玉江等: "新型培养料栽培食用菌研究的现状及展望", 《黑龙江科学》 * |
| 陆娜等: "茶叶渣主要成分及其在三种草腐菌栽培上的应用", 《中国食用菌》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326487A (en) * | 2019-08-06 | 2019-10-15 | 程亚龙 | Composition and its application containing ramulus mori |
| CN112956372A (en) * | 2021-03-11 | 2021-06-15 | 齐齐哈尔大学 | Method for cultivating lucid ganoderma |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103478413B (en) | Method for producing protein feed by mixed-strain solid-state fermentation of ginkgo leaf residues | |
| CN112662566A (en) | Ganoderma lucidum spore-less variety with high yield of polysaccharide and artificial cultivation method thereof | |
| CN101703214A (en) | Lucid Ganoderma hypra powder or Lucid Ganoderma tea and double fermentation process | |
| CN102643864A (en) | Process for preparing yeast cultures | |
| CN102823725B (en) | Method for producing biologic protein feed by using solid-state fermentation fiber dreg | |
| CN112760271B (en) | Process for producing clostridium butyricum through high-density fermentation under negative pressure condition and application | |
| CN109355204A (en) | A kind of method of fermenting and producing cordyceps sinensis mycelium powder | |
| CN102783565B (en) | Preparation method of cordyceps feed additive | |
| CN104543400A (en) | Preparation method of fermentation state zinc feed additive | |
| CN109198167A (en) | Yeast for animal feeds hydrolysate and its preparation method and application | |
| CN109644778A (en) | A kind of edible fungus liquid fermentation medium and preparation method thereof | |
| CN109504725B (en) | Method for preparing high-purity hericium erinaceus polysaccharide by fermenting hericium erinaceus and fermentation culture medium | |
| CN109280632B (en) | Method for producing selenium-rich pleurotus eryngii strains through liquid fermentation | |
| CN114317295A (en) | Liquid culture medium for culturing phellinus igniarius mycelium and culture method thereof | |
| CN104381615B (en) | Produce the method and product of aweto feed addictive | |
| CN110257261A (en) | A kind of production method of selenium-rich antrodia mycelia | |
| CN108977388A (en) | A kind of production method and its used medium of selenium-rich bafillus natto | |
| CN103642695B (en) | One Aspergillus oryzae and the application prepared at fermentable in fodder additives thereof | |
| CN108207492B (en) | Wood-rot type edible fungus liquid strain culture medium, preparation method and application | |
| CN106755179A (en) | A kind of culture medium for being suitable to bacteria cellulose fermentation | |
| CN106035985A (en) | Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine | |
| CN102851328A (en) | Method for preparing citric acid through fermenting corn sugar solution by immobilized Aspergillus niger | |
| CN104429583A (en) | Edible fungus liquid strain culturing method | |
| CN108207493B (en) | Straw rotting type edible fungus liquid strain culture medium, preparation method and application | |
| CN102987058A (en) | Method for producing efficient biological feed by using movable type fermentation and high-energy raw materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190419 |
|
| RJ01 | Rejection of invention patent application after publication |