CN107691109A - A kind of cultural method of ecological ganoderma lucidum - Google Patents
A kind of cultural method of ecological ganoderma lucidum Download PDFInfo
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Abstract
The present invention relates to the cultural method of edible mushroom, and in particular to a kind of cultural method of ecological ganoderma lucidum.The cultural method of the ecological ganoderma lucidum of the present invention includes configuration Medium for Ganoderma lucidum, disinfection inoculation, mycelial growth management, growing season management, ripe bullet powder period management.Wherein, the inventive method is in ganoderma lucidum growing season management, temperature, humidity, gas concentration lwevel, intensity of illumination are included by the environmental factor adjusted by the period in Ecology greenhouse, the condition of accumulation active ingredient is provided for ganoderma lucidum, promote the growth of ganoderma lucidum and the accumulation of GL-B, triterpene and sterol active ingredient, cultivate obtained ganoderma lucidum, the ganoderma polyoses content in its ganoderma lucidum dry product has reached 1.21%, and triterpene and sterol content have reached 2.39%；Compared to cultivating ganoderma is carried out under the conditions of constant environment, the ganoderma lucidum cultivated using the inventive method, ganoderma lucidum quality, which has, to be obviously improved.
Description
【Technical field】
The present invention relates to the cultural method of edible mushroom, and in particular to a kind of cultural method of ecological ganoderma lucidum.
【Background technology】
Ganoderma lucidum is also known as the precious (scientific name of Lin Zhongling, fine jade：Ganoderma Lucidum Karst) it is On Polyporaceae ganoderma lucidum
Fructification.With invigorating qi for tranquilization, it is relieving cough and asthma, promote longevity the effect of.Ganoderma lucidum is also known as polyporus lucidus, refreshing sesame, sesame grass, celestial grass, auspicious
Grass, it is the complete stool of Polyporaceae plant red sesame or purple sesame, ganoderma lucidum originates in east Asia, and distribution in China is most wide in Jiangxi, spirit
Sesame possesses very high medical value, by scientific research institution's number as the Chinese tradition valuable ingredient of traditional Chinese medicine for possessing thousands of years of medicinal histories
The modern pharmacology research of 10 years confirms that ganoderma lucidum adjusts blood glucose, control blood pressure, adjuvant therapy is put for strengthening body immunity
Chemotherapy, liver protecting, sleep etc. is promoted to be respectively provided with significant curative effect.
At present, China Patent Publication No. CN 104115673A, China Patent Publication No. CN 106416747A, are educating sesame mistake
Cheng Zhongjun is carried out educating sesame using constant temperature, the condition of constant humidity, and ganoderma lucidum generation material is planted disclosed in China Patent Publication No. CN 105409596A
In culture method, the technical scheme of regulation of carbon dioxide concentration is additionally used during sesame is gone out, it is suitable to control gas concentration lwevel
The concentration of suitable ganoderma lucidum growth.In existing cultivation of glossy ganoderma technology, optimum condition of the constant environment condition for ganoderma lucidum is used, due to spirit more
Sesame is chronically at the optimum condition of growth, so as to lack the process of effective component of glossy ganoderma accumulation, causes GL-B, triterpenes
The content of compound is relatively low.
【The content of the invention】
The goal of the invention of the present invention is：Relatively low, the cultivation for the effective component of glossy ganoderma content of prior art cultivation
Ganoderma lucidum is chronically at optimum growing condition, the problem of lacking active ingredient cumulative process, there is provided one kind meet ganoderma lucidum growth rhythm,
The cultural method of the high ecological ganoderma lucidum of effective component of glossy ganoderma content.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of cultural method of ecological ganoderma lucidum, comprises the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusted after natural cooling using sterilized water aqueous
Measure as 55-65%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains Ganoderma Lucidum
Bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, is controlled in darkroom
Temperature be 22-24 DEG C, air humidity 67-72%, the concentration of intermittent aeration regulation of carbon dioxide be 500-650ppm, cultivate
Stem is grown to ganoderma lucidum；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, solution is degerming
Bag sealing, the moisture for keeping culture medium in bacterium bag is 55-65%, and the temperature in the Ecology greenhouse is 18-28 DEG C, institute
State that the humidity in Ecology greenhouse is 83-93%, the gas concentration lwevel in the Ecology greenhouse is 150-250ppm, described
Intensity of illumination in Ecology greenhouse is 0-8000lx, cultivates ganoderma lucidum in such circumstances；
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and the humidity reduced in greenhouse is 68-72%, dense carbon dioxide
600-1000ppm is spent, carries out lucidum spore powder collection, ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
Further, in the Medium for Ganoderma lucidum, by weight, 80-95 parts ramulus mori, 0.5-3 parts gypsum, 2-20 are contained
Part wheat bran, the decomposed sweet wormwood slag of 8-12 parts, and carry out the packing by every bag of 0.9-1.4kg.
Further, the granular size of the ramulus mori is 15-25 mesh, and is soaked in water before the Medium for Ganoderma lucidum is prepared
20-28 hours.
Further, the temperature in the Ecology greenhouse is adjusted by the period, specific method following 7：00-9：00,
It is 25-27 DEG C to adjust temperature；9：00-15：00, regulation temperature is 26-28 DEG C；15：00-19：00 regulation temperature is 28-30 DEG C；
19：00- next day 7：00, regulation temperature is 18-23 DEG C.
Further, the humidity in the Ecology greenhouse is adjusted by the period, specific method following 7：00-9：00,
Regulation humidity is 90-93%；9：00-15：00, regulation humidity is 86-90%；15：00-19：00, regulation humidity is 83-86%；
19：00- next day 7：00, regulation humidity is 87-91%.
Further, the gas concentration lwevel in the Ecology greenhouse is adjusted by the period, specific method following 7：
00-9：00, regulation of carbon dioxide concentration is 200-230ppm；9：00-15：00, regulation of carbon dioxide concentration is 150-180ppm；
15：00-19：00, regulation of carbon dioxide concentration is 180-200ppm；19：00- next day 7：00, regulation of carbon dioxide concentration is
230-250ppm。
Further, the illumination in the Ecology greenhouse is adjusted by the period, specific method following 7：00-9：00,
Regulating illumination intensity is 3500-5000lx；9：00-15：00, regulating illumination intensity 6500-8000lx；15：00-19：00, adjust
Section intensity of illumination is 5000-6500lx；19：00- next day 7：00, regulating illumination intensity 0-20lx.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. the inventive method employs ecological cultivation method, growth conditions of the ganoderma lucidum in virgin forest environment are simulated, most
The environment of the reproduction nature of big degree, by the growth conditions such as temperature, humidity, the carbon dioxide that strictly control cultivation of glossy ganoderma
Concentration, intensity of illumination, make a kind of environment of most suitable ganoderma lucidum growth.Ganoderma lucidum growing environment provided by the invention is relative to tradition
Cultivated under the environment such as constant temperature and humidity, be more beneficial for the accumulation of ganoderma lucidum active ingredient in growth course, the inventive method culture
The ganoderma polyoses content of ganoderma lucidum fruitbody is up to 1.21%, and triterpenes content is up to 2.35%, relative to can effectively carrying for tradition plantation
It is high by more than 50%.On the other hand, relative to the ganoderma lucidum of transplanting woodland plantation, it is easier to manage in Ecology greenhouse, avoids certainly
Right disaster causes the underproduction and yield unstable.
2. the inventive method in continuous research and probe, has been invented a kind of according to different time sections progress dynamic control spirit
The method of sesame growing environment, dynamic regulation temperature, humidity, gas concentration lwevel, the environmental factor of intensity of illumination, suitable ganoderma lucidum
Growth and active ingredient accumulation.9：00-15：00, the environmental factor that the present invention is adjusted in Ecology greenhouse is most suitable spirit
The condition of sesame growth, be advantageous to the fast-growth of ganoderma lucidum；The development of ganoderma lucidum fruitbody is very sensitive to light, although from no light
Fructification can be produced to strong light, but growth of the different illumination intensity to ganoderma lucidum has a great impact, inventor is in lucidum seed
Found during plant：With the Low light intensity 50-1000lx, high intensity of illumination 6500-8000lx used in existing cultivation technique
The growth of ganoderma lucidum is more beneficial for, the synthesis of the yield and active ingredient of ganoderma lucidum can be improved.19：00- next day 7：00, the present invention
Adjust growth of the Ecology greenhouse to low temperature, high humility, high carbon dioxide concentration and nothing/Low light intensity condition, now ganoderma lucidum
Speed slows down, and is advantageous to the accumulation of active ingredient in ganoderma lucidum, to improve the quality of ganoderma lucidum.The present invention plays the powder phase in ganoderma lucidum maturation,
Higher gas concentration lwevel 600-1000ppm and relatively low humidity 68-72% is provided with Ecology greenhouse, in this condition
Under, promote the maturation of ganoderma lucidum and play powder, conidia powder fresh goods yield can reach 17.69g/ bags.Planted using the inventive method
The ganoderma lucidum of training, compared to existing cultivation of glossy ganoderma technology, its polyoses content improves about 26%, and triterpene and sterol content improve 50%
Left and right, tool increase significantly.
【Embodiment】
With reference to embodiment, the invention will be further described.
Embodiment 1
A kind of cultural method of ecological ganoderma lucidum, comprises the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
Wherein, in Medium for Ganoderma lucidum, by weight, 80 parts of ramulus moris, 0.5 part of gypsum, 2 parts of wheat bran, 8 parts of decomposed green grass or young crops are contained
Wormwood artemisia slag, and carry out the packing by every bag of 0.9kg；Wherein, the granular size of the ramulus mori is 15 mesh, and is preparing the ganoderma lucidum
It is soaked in water before culture medium 20 hours.
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusted after natural cooling using sterilized water aqueous
Measure as 55%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains ganoderma lucidum bacterium bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, is controlled in darkroom
Temperature be 22 DEG C, air humidity 67%, the concentration of intermittent aeration regulation of carbon dioxide is 500ppm, cultivates to ganoderma lucidum and grows
Stem；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, solution is degerming
Bag sealing, the moisture for keeping culture medium in bacterium bag are 55%, and the temperature in the Ecology greenhouse is 18-28 DEG C, described
Humidity in Ecology greenhouse is 83-90%, the gas concentration lwevel in the Ecology greenhouse is 150-230ppm, described imitative
Intensity of illumination in Ecological Greenhouse is 0-6500lx, cultivates ganoderma lucidum in such circumstances；
Wherein, the temperature in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Temperature be 24 DEG C, 9：00-15：00 adjusts temperature as 28 DEG C, 15：00-19：00 adjusts temperature as 28 DEG C, 19：00- next day 7：00
It is 18 DEG C to adjust temperature；Humidity in the Ecological Greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Humidity is 90%, 9：00-15：00 regulation humidity is 86%, 15：00-19：00 regulation humidity is 83%, 19：00- next day 7：00
It is 87% to adjust humidity.Gas concentration lwevel in the Ecological Greenhouse is adjusted by the period, and specific method is as follows：7：00-
9：00 regulation of carbon dioxide concentration is 200ppm, 9：00-15：00 regulation of carbon dioxide concentration is 150ppm, 15：00-19：00 adjusts
Section gas concentration lwevel is 180ppm, 19：00- next day 7：00 regulation of carbon dioxide concentration is 230ppm.
Wherein, the intensity of illumination in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00
Regulating illumination intensity is 3500lx, 9：00-15：00 regulating illumination intensity is 6500lx, 15：00-19：00 regulating illumination intensity is
5000lx,19：00- next day 7：00 regulating illumination intensity is 0lx.
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and it is 68% to reduce the humidity in greenhouse, gas concentration lwevel
600ppm, carries out lucidum spore powder collection, and ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
Embodiment 2
A kind of cultural method of ecological ganoderma lucidum, comprises the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
Wherein, in the Medium for Ganoderma lucidum, by weight, containing 82 parts of ramulus moris, 1 part of gypsum, 6 parts of wheat bran, 9 parts it is decomposed
Sweet wormwood slag, and carry out the packing by every bag of 1kg；Wherein, the granular size of the ramulus mori is 17 mesh, and is preparing the ganoderma lucidum
It is soaked in water before culture medium 22 hours.
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusted after natural cooling using sterilized water aqueous
Measure as 57%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains ganoderma lucidum bacterium bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, is controlled in darkroom
Temperature be 23 DEG C, air humidity 69%, the concentration of intermittent aeration regulation of carbon dioxide is 530ppm, cultivates to ganoderma lucidum and grows
Stem；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, solution is degerming
Bag sealing, the moisture for keeping culture medium in bacterium bag are 58%, and the temperature in the Ecology greenhouse is 19-29 DEG C, described
Humidity in Ecology greenhouse is 84-91%, the gas concentration lwevel in the Ecology greenhouse is 156-235ppm, described imitative
Intensity of illumination in Ecological Greenhouse is 5-6900lx, cultivates ganoderma lucidum in such circumstances；
Wherein, the temperature in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Temperature be 24 DEG C, 9：00-15：00 adjusts temperature as 27 DEG C, 15：00-19：00 adjusts temperature as 29 DEG C, 19：00- next day 7：00
It is 19 DEG C to adjust temperature.Humidity in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 adjusts
It is 91%, 9 to save humidity：00-15：00 regulation humidity is 87%, 15：00-19：00 regulation humidity is 84%, 19：00- next day 7：
00 regulation humidity is 88%.Gas concentration lwevel in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：
00-9：00 regulation of carbon dioxide concentration is 206ppm, 9：00-15：00 regulation of carbon dioxide concentration is 156ppm, 15：00-19：
00 regulation of carbon dioxide concentration is 185ppm, 19：00- next day 7：00 regulation of carbon dioxide concentration is 235ppm.
Wherein, the illumination in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Intensity of illumination is 3900lx, 9：00-15：00 regulating illumination intensity is 6900lx, 15：00-19：00 regulating illumination intensity is
5400lx,19：00- next day 7：00 regulating illumination intensity is 5lx.
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and it is 69% to reduce the humidity in greenhouse, gas concentration lwevel
700ppm, carries out lucidum spore powder collection, and ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
Embodiment 3
A kind of cultural method of ecological ganoderma lucidum, comprises the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
Wherein, in the Medium for Ganoderma lucidum, by weight, containing 90 parts of ramulus moris, 1.5 parts of gypsum, 10 parts of wheat bran, 10 parts
Decomposed sweet wormwood slag, and carry out the packing by every bag of 1.2kg；Wherein, the granular size of the ramulus mori is 20 mesh, and is preparing institute
It is soaked in water 24 hours before stating Medium for Ganoderma lucidum.
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusted after natural cooling using sterilized water aqueous
Measure as 60%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains ganoderma lucidum bacterium bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, is controlled in darkroom
Temperature be 23 DEG C, air humidity 70%, the concentration of intermittent aeration regulation of carbon dioxide is 570ppm, cultivates to ganoderma lucidum and grows
Stem；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, solution is degerming
Bag sealing, the moisture for keeping culture medium in bacterium bag are 60%, and the temperature in the Ecology greenhouse is 20-29 DEG C, described
Humidity in Ecology greenhouse is 85-92%, the gas concentration lwevel in the Ecology greenhouse is 165-240ppm, described imitative
Intensity of illumination in Ecological Greenhouse is 10-7300lx, cultivates ganoderma lucidum in such circumstances；
Wherein, the temperature in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Temperature be 25 DEG C, 9：00-15：00 adjusts temperature as 27 DEG C, 15：00-19：00 adjusts temperature as 29 DEG C, 19：00- next day 7：00
It is 20 DEG C to adjust temperature.Humidity in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 adjusts
It is 92%, 9 to save humidity：00-15：00 regulation humidity is 88%, 15：00-19：00 regulation humidity is 85%, 19：00- next day 7：
00 regulation humidity is 89%.Gas concentration lwevel in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：
00-9：00 regulation of carbon dioxide concentration is 215ppm, 9：00-15：00 regulation of carbon dioxide concentration is 165ppm, 15：00-19：
00 regulation of carbon dioxide concentration is 190ppm, 19：00- next day 7：00 regulation of carbon dioxide concentration is 240ppm.
Wherein, the illumination in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Intensity of illumination is 4300lx, 9：00-15：00 regulating illumination intensity is 7300lx, 15：00-19：00 regulating illumination intensity is
5800lx,19：00- next day 7：00 regulating illumination intensity is 10lx.
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and it is 70% to reduce the humidity in greenhouse, gas concentration lwevel
800ppm, carries out lucidum spore powder collection, and ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
Embodiment 4
A kind of cultural method of ecological ganoderma lucidum, comprises the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
Wherein, in the Medium for Ganoderma lucidum, by weight, 92 parts of ramulus moris, 2 parts of gypsum, 15 parts of wheat bran, 11 parts of corruption are contained
Ripe sweet wormwood slag, and carry out the packing by every bag of 1.3kg；Wherein, the granular size of the ramulus mori is 23 mesh, and described in preparation
It is soaked in water before Medium for Ganoderma lucidum 26 hours.
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusted after natural cooling using sterilized water aqueous
Measure as 55-65%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains Ganoderma Lucidum
Bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, is controlled in darkroom
Temperature be 24 DEG C, air humidity 71%, the concentration of intermittent aeration regulation of carbon dioxide is 610ppm, cultivates to ganoderma lucidum and grows
Stem；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, solution is degerming
Bag sealing, the moisture for keeping culture medium in bacterium bag are 63%, and the temperature in the Ecology greenhouse is 22-29 DEG C, described
Humidity in Ecology greenhouse is 86-92%, the gas concentration lwevel in the Ecology greenhouse is 175-245ppm, described imitative
Intensity of illumination in Ecological Greenhouse is 15-7500lx, cultivates ganoderma lucidum in such circumstances；
Wherein, the temperature in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Temperature be 26 DEG C, 9：00-15：00 adjusts temperature as 26 DEG C, 15：00-19：00 adjusts temperature as 29 DEG C, 19：00- next day 7：00
It is 22 DEG C to adjust temperature.Humidity in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 adjusts
It is 92%, 9 to save humidity：00-15：00 regulation humidity is 89%, 15：00-19：00 regulation humidity is 86%, 19：00- next day 7：
00 regulation humidity is 91%.Gas concentration lwevel in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：
00-9：00 regulation of carbon dioxide concentration is 225ppm, 9：00-15：00 regulation of carbon dioxide concentration is 175ppm, 15：00-19：
00 regulation of carbon dioxide concentration is 195ppm, 19：00- next day 7：00 regulation of carbon dioxide concentration is 245ppm.
Wherein, the illumination in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Intensity of illumination is 4500lx, 9：00-15：00 regulating illumination intensity is 7500lx, 15：00-19：00 regulating illumination intensity is
6200lx,19：00- next day 7：00 regulating illumination intensity is 15lx.
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and it is 71% to reduce the humidity in greenhouse, gas concentration lwevel
900ppm, carries out lucidum spore powder collection, and ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
Embodiment 5
A kind of cultural method of ecological ganoderma lucidum, comprises the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
Wherein, in the Medium for Ganoderma lucidum, by weight, 95 parts of ramulus moris, 3 parts of gypsum, 20 parts of wheat bran, 12 parts of corruption are contained
Ripe sweet wormwood slag, and carry out the packing by every bag of 1.4kg；Wherein, the granular size of the ramulus mori is 25 mesh, and described in preparation
It is soaked in water before Medium for Ganoderma lucidum 28 hours.
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusted after natural cooling using sterilized water aqueous
Measure as 65%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains ganoderma lucidum bacterium bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, is controlled in darkroom
Temperature be 24 DEG C, air humidity 72%, the concentration of intermittent aeration regulation of carbon dioxide is 650ppm, cultivates to ganoderma lucidum and grows
Stem；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, solution is degerming
Bag sealing, the moisture for keeping culture medium in bacterium bag are 65%, and the temperature in the Ecology greenhouse is 23-30 DEG C, described
Humidity in Ecology greenhouse is 86-93%, the gas concentration lwevel in the Ecology greenhouse is 180-250ppm, described imitative
Intensity of illumination in Ecological Greenhouse is 20-8000lx, cultivates ganoderma lucidum in such circumstances；
Temperature in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00, which adjusts temperature, is
26 DEG C, 9：00-15：00 adjusts temperature as 28 DEG C, 15：00-19：00 adjusts temperature as 30 DEG C, 19：00- next day 7：00 regulation temperature
Spend for 23 DEG C.Humidity in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation humidity
For 93%, 9：00-15：00 regulation humidity is 90%, 15：00-19：00 regulation humidity is 86%, 19：00- next day 7：00 regulation
Humidity is 91%.Gas concentration lwevel in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：
00 regulation of carbon dioxide concentration is 230ppm, 9：00-15：00 regulation of carbon dioxide concentration is 180ppm, 15：00-19：00 regulation
Gas concentration lwevel is 200ppm, 19：00- next day 7：00 regulation of carbon dioxide concentration is 250ppm.
Wherein, the illumination in the Ecology greenhouse is adjusted by the period, and specific method is as follows：7：00-9：00 regulation
Intensity of illumination is 5000lx, 9：00-15：00 regulating illumination intensity is 8000lx, 15：00-19：00 regulating illumination intensity is
6500lx,19：00- next day 7：00 regulating illumination intensity is 20lx.
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and it is 72% to reduce the humidity in greenhouse, gas concentration lwevel
1000ppm, carries out lucidum spore powder collection, and ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
Embodiment 6
With reference to the scheme in a kind of ganoderma lucidum substituting stuff cultivation method embodiments of China Patent Publication No. CN 10540959A, carry out
Cultivation of glossy ganoderma.
Embodiment 7
With reference to the cultural method of the ecological ganoderma lucidum of the embodiment of the present invention 3, distinguish in ganoderma lucidum growing season management, imitating
28 DEG C of progress cultivation of glossy ganoderma of constant temperature, remaining step are identical in Ecological Greenhouse.
Embodiment 8
With reference to the cultural method of the ecological ganoderma lucidum of the embodiment of the present invention 3, distinguish in ganoderma lucidum growing season management, imitating
It is 88% progress cultivation of glossy ganoderma that humidity is kept in Ecological Greenhouse, and remaining step is identical.
Embodiment 9
With reference to the cultural method of the ecological ganoderma lucidum of the embodiment of the present invention 3, distinguish in ganoderma lucidum growing season management, imitating
It is that 190ppm carries out cultivation of glossy ganoderma that gas concentration lwevel is kept in Ecological Greenhouse, and remaining step is identical.
Embodiment 10
With reference to the cultural method of the ecological ganoderma lucidum of the embodiment of the present invention 3, distinguish in ganoderma lucidum growing season management, imitating
It is that 7300lx carries out cultivation of glossy ganoderma that intensity of illumination is kept in Ecological Greenhouse, and remaining step is identical.
Embodiment 11
Embodiment 1-10 is cultivated to have obtained ganoderma lucidum and is prepared as ganoderma lucidum dry product, wherein embodiment 1-5 is respectively labeled as sample
1-5,
Embodiment 6-10 is respectively labeled as comparative sample 1-5, with reference in《Chinese Pharmacopoeia》In the 188-189 pages of versions in 2015
The assay method of ganoderma polyoses content and triterpene and sterol content, determination sample 1-5 and comparative sample 1-5 ganoderma polyoses content
With triterpene and sterol content, and average content is calculated respectively；Meanwhile the ganoderma lucidum Dried product of each bacterium bag is counted, calculate every bag
The ganoderma lucidum Dried product of bacterium bag；Measurement result is shown in Table 1.
The ganoderma lucidum sample measurement result of table 1
As can be seen from the above table, the ganoderma lucidum sample 1-5 cultivated using the inventive method, the contrast cultivated compared with prior art
Sample 1, its ganoderma polyoses content improve about 26%, and triterpene and sterol content improve about 51%, and ganoderma lucidum Dried product improves
About 37%, tool increases significantly, it was demonstrated that the cultural method of ecological ganoderma lucidum of the invention is equal to the indices of ganoderma lucidum product
With being obviously improved.The contrast of constant temperature, constant humidity, permanent gas concentration lwevel, permanent intensity of illumination is respectively adopted in ganoderma lucidum growth period
Sample 2-5, the GL-B of these comparative samples compares sample 3 with triterpene and sterol content the decline of different amplitudes, especially
It is the ganoderma lucidum cultivated using constant carbon dioxide and constant illumination, and ganoderma polyoses content have dropped about 14.9% He respectively
12.3%, constant environmental factor is unfavorable for the accumulation of effective component of glossy ganoderma, but little to the yield effect of ganoderma lucidum.Therefore use
The ganoderma lucidum that the inventive method is cultivated, there is higher GL-B, triterpene and sterol content, compared with prior art ganoderma lucidum
Quality and yield, which all have, to be obviously improved.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of cultural method of ecological ganoderma lucidum, it is characterised in that comprise the following steps：
(1) prepare culture medium and dispensed, obtain Medium for Ganoderma lucidum；
(2) disinfection inoculation：The Medium for Ganoderma lucidum is subjected to normal-pressure sterilization, adjusting water content using sterilized water after natural cooling is
55-65%, cultivation of glossy ganoderma strain is inscribed to the Medium for Ganoderma lucidum after sterilizing using sterile working and sealed, obtains ganoderma lucidum bacterium bag；
(3) cultivation of glossy ganoderma management：
A. mycelial growth management：Ganoderma lucidum bacterium bag after being inoculated with (2) is put into darkroom and carries out lucifuge culture, controls the temperature in darkroom
Spend for 22-24 DEG C, air humidity 67-72%, the concentration of intermittent aeration regulation of carbon dioxide is 500-650ppm, is cultivated to spirit
Sesame grows stem；
B. growing season management：The bacterium bag after stem will be grown and moved into from darkroom in Ecology greenhouse and continue to plant, release bacterium bag envelope
Mouthful, the moisture for keeping culture medium in bacterium bag be 55-65%, and the temperature in the Ecology greenhouse is 18-28 DEG C, described is imitated
Humidity in Ecological Greenhouse is 83-93%, the gas concentration lwevel in the Ecology greenhouse is 150-250ppm, described bionical
Intensity of illumination in state greenhouse is 0-8000lx, cultivates ganoderma lucidum in such circumstances；
C. it is ripe to play powder period management：Ganoderma lucidum starts after playing powder, and the humidity adjusted in Ecology greenhouse is 68-72%, carbon dioxide
Concentration 600-1000ppm, carries out lucidum spore powder collection, and ganoderma lucidum stops that after playing powder ganoderma lucidum can be harvested.
A kind of 2. cultural method of ecological ganoderma lucidum according to claim 1, it is characterised in that in the Medium for Ganoderma lucidum,
By weight, containing 80-95 parts ramulus mori, 0.5-3 parts gypsum, 2-20 parts wheat bran, the decomposed sweet wormwood slag of 8-12 parts, and by every bag
0.9-1.4kg carries out the packing.
A kind of 3. cultural method of ecological ganoderma lucidum according to claim 2, it is characterised in that the granular size of the ramulus mori
For 15-25 mesh, and it is soaked in water 20-28 hours before the Medium for Ganoderma lucidum is prepared.
4. the cultural method of a kind of ecological ganoderma lucidum according to claim 1, it is characterised in that in the Ecology greenhouse
Temperature is adjusted by the period, specific method following 7：00-9：00, regulation temperature is 25-27 DEG C；9：00-15：00, regulation temperature
Spend for 26-28 DEG C；15：00-19：00, regulation temperature is 28-30 DEG C；19：00- next day 7：00, regulation temperature is 18-23 DEG C.
5. the cultural method of a kind of ecological ganoderma lucidum according to claim 1, it is characterised in that in the Ecology greenhouse
Humidity is adjusted by the period, specific method following 7：00-9：00, regulation humidity is 90-93%；9：00-15：00, regulation is wet
Spend for 86-90%；15：00-19：00, regulation humidity is 83-86%；19：00- next day 7：00, regulation humidity is 87-91%.
6. the cultural method of a kind of ecological ganoderma lucidum according to claim 1, it is characterised in that in the Ecology greenhouse
Gas concentration lwevel is adjusted by the period, specific method following 7：00-9：00, regulation of carbon dioxide concentration is 200-
230ppm；9：00-15：00, regulation of carbon dioxide concentration is 150-180ppm；15：00-19：00, regulation of carbon dioxide concentration is
180-200ppm；19：00- next day 7：00, regulation of carbon dioxide concentration is 230-250ppm.
7. the cultural method of a kind of ecological ganoderma lucidum according to claim 1, it is characterised in that in the Ecology greenhouse
Illumination is adjusted by the period, specific method following 7：00-9：00, regulating illumination intensity 3500-5000lx；9：00-15：
00, regulating illumination intensity 6500-8000lx；15：00-19：00, regulating illumination intensity 5000-6500lx；19：00- next day
7：00, regulating illumination intensity 0-20lx.
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| CN109089727A (en) * | 2018-08-09 | 2018-12-28 | 合肥福泉现代农业科技有限公司 | A kind of method of biomimetic environment illumination cultivation Agricus blazei |
| CN109168975A (en) * | 2018-10-09 | 2019-01-11 | 湖北悟芝堂生物科技有限公司 | A kind of ganoderma lucidum cultivation method |
| CN109168974A (en) * | 2018-10-09 | 2019-01-11 | 湖北悟芝堂生物科技有限公司 | A kind of Wildmimic cultivation method of ganoderma lucidum |
| CN109644778A (en) * | 2018-12-27 | 2019-04-19 | 广西仙草堂制药有限责任公司 | A kind of edible fungus liquid fermentation medium and preparation method thereof |
| CN114085781A (en) * | 2021-11-24 | 2022-02-25 | 贵州省土壤肥料研究所(贵州省生态农业工程技术研究中心)(贵州省农业资源与环境研究所) | Ganoderma GZ and application thereof |
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| CN114085781A (en) * | 2021-11-24 | 2022-02-25 | 贵州省土壤肥料研究所(贵州省生态农业工程技术研究中心)(贵州省农业资源与环境研究所) | Ganoderma GZ and application thereof |
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