CN1594545A - Method for promoting and inducing generation and accumulation of ginseng secondary metabolite - Google Patents
Method for promoting and inducing generation and accumulation of ginseng secondary metabolite Download PDFInfo
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- CN1594545A CN1594545A CN 200410027685 CN200410027685A CN1594545A CN 1594545 A CN1594545 A CN 1594545A CN 200410027685 CN200410027685 CN 200410027685 CN 200410027685 A CN200410027685 A CN 200410027685A CN 1594545 A CN1594545 A CN 1594545A
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Abstract
The invention relates to a method of promoting and inducing lucid ganoderma to produce and accumulate secondary metabolite. Environmental stimulus and regulation are given to lucid ganoderma during pileus and basidiospore development period, thus secondary metabolite triterpene is produced and accumulated. Analysis shows that lucid ganoderma triterpene content of spore is high and reaches to 3.2-4.5%.
Description
Technical field
The present invention relates to a kind of growth and development stage that differentiates cap and produce basidiospore glossy ganoderma and give that environmental factor stimulates and regulation and control, promote glossy ganoderma to grow and induce the method for generation and the accumulation of glossy ganoderma secondary metabolite ganoderma lucidum triterpene compounds.
Background technology
Glossy ganoderma (Ganoderma lucidum) 1 year or perennial fungi are saprophytic bacteria, owing to can colonize on the tree that lives, so be called again facultative parasite.The Shennong's Herbal of the Eastern Han Dynasty is classified glossy ganoderma as top grade medicine, and the Compendium of Material Medica of Ming Dynasty's LI Shi-Zhen is more detailed to the record of glossy ganoderma.The successive dynasties physician thinks that all glossy ganoderma can Zhiduo County plants disease, is nourishing and fit keeping function, the precious medicine of strengthening the body resistance to consolidate the constitution, promoting longevity.Modern medicine makes substantial progress to effective component of glossy ganoderma analysis, pharmacology and clinical application research,, immunomodulatory anti-ageing to glossy ganoderma, pharmacological action and mechanism of action antitumor and that activate blood circulation and disperse blood clots carry out big quantity research, wherein the immunostimulation of ganoderan is considered to the secret that glossy ganoderma " is strengthened the body resistance to consolidate the constitution ", particularly sporoderm-broken Ganoderma spore is applied to the clinical efficacy of tumor chemoradiotherapy assisting therapy, enjoys Chinese scholars to attract attention.
Chemical research development to glossy ganoderma over nearly 30 years is very fast, has obtained nearly 200 kinds of compounds from the Ganoderma fungi, such as ganoderma lucidum triterpene compounds, polysaccharide, nucleoside compound, furfuran compound, phytosterin compound, alkaloid and polypeptide amino acid class.Especially ganoderma lucidum triterpene compounds has in inhibiting HIV carrying capacity CD8 in pharmaceutical research and clinical practice immune indexes CD4 is effectively promoted, and have not only inhibition tumor cell growth, but also strengthen antitumor radiotherapy and chemotherapy medicine curative effect and can reduce the pharmacologically active of toxic and side effects of chemoradiotherapy revealed after, in recent years to the research of glossy ganoderma quality and quality and ganoderma lucidum triterpene compounds development of resources and separating-purifying subject study are become new study hotspot.
Summary of the invention
The purpose of this invention is to provide a kind of growth and development stage that differentiates cap and produce basidiospore glossy ganoderma and give that environmental factor stimulates and regulation and control, promote glossy ganoderma to grow and induce the method for generation and the accumulation of glossy ganoderma secondary metabolite ganoderma lucidum triterpene compounds.
Discover; ganoderma lucidum triterpene compounds is that the glossy ganoderma process of growth is excited by environmental factors to induce to produce and the secondary metabolite of accumulation, makes to resist and to adapt to that its gene is stable not to morph and earn a bare living the protection material that organism was produced under extreme environment.Analysis revealed, the physical environment down glossy ganoderma of growth contain ganoderma lucidum triterpene compounds and mainly are present in the Ganoderma spore as the seed that goes down to posterity, look quality discrepancy content and are about 0.5-3%; Then mainly concentrate on the periphery position of glossy ganoderma cap in the sporophore, and increase progressively along with the raising of its ripening degree; The mycelium that the employing deep layer is cultivated or extremely low with the contained triterpene compound content of the glossy ganoderma of cultivation on sawdust.
The inventor finds that through experiment the glossy ganoderma of artificial culture and Reishi sporule quality, the selection of culture base-material and nurturing an environment are crucial.In glossy ganoderma differential period if the oxygen deficiency, nurturing an environment CO
2Content surpasses 0.1%, the stasi of glossy ganoderma cap, glossy ganoderma stem formation " deer horn " glossy ganoderma that grows tall fast.As utilize culture base-materials such as wood chip, maize spike stalk, bagasse to cultivate glossy ganoderma, and though the glossy ganoderma presentation of results and common glossy ganoderma no significant difference, Ganoderma sporophore quality pine is flat, and Ganoderma spore is shrivelled light, and the basidiospore germination rate is extremely low; As select for use hard section wood of deciduous tree and good nurturing an environment to cultivate glossy ganoderma, and the glossy ganoderma cap stem quality of results is abundant, and toughness is strong, and Ganoderma spore is full to enrich contained ganoderma lucidum triterpene compounds content height, basidiospore germination rate height.
Method of the present invention is to differentiate cap glossy ganoderma to give environmental factors stimulation and regulation and control with the growth and development stage that produces sporidium, comprises by regulation and control different light medium and light application time, difference variation, humidity variation and CO
2These environmental factorss of content stimulate the generation and the accumulation of inducing Ganoderma spore secondary metabolite ganoderma lucidum triterpene compounds; The contained ganoderma lucidum triterpene compounds content of the Ganoderma spore that is obtained is up to 3.2-4.5%.
The concrete steps of method of the present invention are as follows:
One, differentiates the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, to obtain the illumination of orange colour scattered light; Also can use the orange colour light source bulb irradiation prolonging light time, intensity of illumination is the 1500-5000 Lux again.
Two, producing the growth and development stage of basidiospore glossy ganoderma, generally is to reach about 6cm at the glossy ganoderma bacteria cover diameter, adopts the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, to obtain black-and-blue scattered light illumination; Also can be again with the black-and-blue light source bulb irradiation prolonging light time, intensity of illumination is the 2000-6000 Lux.
Three, in the Ganoderma sporophore stage of maturity, generally be to reach about 8cm at the glossy ganoderma bacteria cover diameter, can at dusk the time-division be cultivated the booth ventilation opening with glossy ganoderma and the door curtain that keeps out the wind opens wide in every day, make ventilating convection promote glossy ganoderma to cultivate the interior room temperature of booth and descend rapidly, room temperature is 4-12 ℃ in the booth so that regulation and control glossy ganoderma at night is cultivated; Can glossy ganoderma be cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed in early morning every day, room temperature is gone up, room temperature is 25-32 ℃ in the booth so that regulation and control glossy ganoderma on daytime is cultivated; Simultaneously, it is 65-75% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and can produce CO by adopted common censer light the straw fumigation at every interval 60-80 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.1-0.3%.
Four, the regulation and control glossy ganoderma of gathering during the Ganoderma spore cultivates in the booth round the clock that room temperature is 24-27 ℃.The fresh Reishi sporule that preferably will spray the same day was in time gathered at dusk and drying on the same day.Analysis revealed, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is up to 3.2-4.5%.
The inventive method is on the basis of the method for conventional artificial greenhouse cultivation glossy ganoderma, reaches purpose of the present invention by above-mentioned technique improvement.
According to the method for the invention described above, other specific procedure that glossy ganoderma is cultivated such as the lucidum strain production of hybrid seeds, the wooden selection of section and detection, the stake inoculation of section wood fungus and send out that bacterium is cultivated, the place is selected and build glossy ganoderma cultivate that booth, glossy ganoderma cultivating and managing, Reishi sporule gather etc. can be with reference to conventional method.
According to the method for the invention described above, the place is selected to utilize the higher mountain region of height above sea level, selects to build glossy ganoderma away from the highland that ventilates in the pollution-free source in the signs of human habitation and roadside on the sunny side and cultivates booth, be convenient to round the clock between glossy ganoderma cultivate and form the big temperature difference in the booth; Preferably select the wooden hard section wood such as broad leaf tree such as rice sweet oak, evergreen chinquapin, maple, robur, plum to cultivate glossy ganoderma, and the wooden middle heavy metal of sampling detection segment wood and agricultural residual content, the Duan Mu that strict control is selected meets common cultivation organic culture medium of edible fungus material heavy metal and agricultural residual content's index; Control is used for the interior relative humidity of regulation and control glossy ganoderma cultivation booth and the shower water of glossy ganoderma nurturing period is selected free of contamination mountain spring water or clear water.
Test of the present invention shows that different light medium and light application time, difference variation and humidity change, CO
2The stimulation glossy ganoderma is grown environmental factorss such as content and the ganoderma lucidum triterpene compounds content presents otherness.Differentiate the growth and development stage of cap glossy ganoderma, the illumination of orange colour scattered light and prolonging light time are provided, can promote the glossy ganoderma cap to thicken, glossy ganoderma output is significantly increased production; At the growth and development stage of glossy ganoderma generation basidiospore, changing light quality is black-and-blue scattered light and prolonging light time, can promote glossy ganoderma to produce a large amount of basidiospore; Room temperature is 24-27 ℃ in the regulation and control glossy ganoderma cultivation booth during the Reishi sporule of gathering, and it is vigorous to promote that Reishi sporule sprays; In the ganoderma lucidum fruitbody stage of ripeness, in the regulation and control glossy ganoderma nurturing an environment round the clock between temperature difference amplitude, humidity and CO
2Content also forms extreme environment, can stimulate generation and the accumulation of inducing Reishi sporule secondary metabolite ganoderma lucidum triterpene compounds, and the Reishi sporule quality significantly improves, and ganoderma lucidum triterpene compounds content is up to 3.2-4.5% in the Reishi sporule.Through further processing, can be used as anti-AIDS and tumor chemoradiotherapy medicine and adjuvant therapy medicaments and be applied to clinical.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 4000-4500 Lux.
4, reach about 5.9cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3500-4000 Lux.
5, reach about 8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 4-7 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 26-27 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 65-70% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 60-65 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.1-0.15%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 24-26 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.3-4.5%.
Embodiment two:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source in the signs of human habitation and roadside of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of maple; Get free of contamination clear water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light.
4, reach about 6cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination.
5, reach about 8.1cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature drops to 10-12 ℃ rapidly in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 29-32 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 70-75% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 75-80 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.25-0.3%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 26-27 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 3.5-3.6%.
Embodiment three:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of maple; Get free of contamination mountain spring water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3500 Lux.
4, reach about 5.8cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3000-4200 Lux.
5, reach about 8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 4-8 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 25-28 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 68-72% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 60-70 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.1-0.2%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 24-25 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.2-4.4%.
Embodiment four:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 3500-4500 Lux.
4, reach about 6cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3800 Lux.
5, reach about 8.2cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 6-11 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 27-31 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 69-74% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 60-65 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.15-0.2%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 25-27 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.1-4.3%.
Embodiment five:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of robur; Get free of contamination mountain spring water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 3000-5000 Lux.
4, reach about 6cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination.Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 2500-3500 Lux.
5, reach about 8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 5-10 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 28-32 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 70-74% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 62-75 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.1-0.15%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 25-27 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.0-4.3%.
Embodiment six:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of Japanese apricot; Get free of contamination mountain spring water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 1500-3000 Lux.
4, reach about 6cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3500-6000 Lux.
5, reach about 8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 7-12 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 26-31 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 69-72% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 68-78 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.2-0.3%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 24-26 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.3-4.5%.
Embodiment seven:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 1700-3500 Lux.
4, reach about 6cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 4500-5800 Lux.
5, reach about 7.9cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 5-9 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 30-32 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 69-75% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 66-76 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.2-0.25%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 24-25 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.2-4.4%.
Embodiment eight:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select qualified after testing hard section wood of rice sweet oak tree; Get free of contamination mountain spring water and cultivate relative humidity and the spray water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-5000 Lux.
4, reach about 5.9cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3000-4500 Lux.
5, reach about 8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature is 9-12 ℃ in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 29-32 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 66-73% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 60-65 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.15-0.25%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 25-27 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 4.2-4.5%.
Embodiment nine:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source in the signs of human habitation and roadside of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of robur; Get free of contamination clear water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, can obtain the illumination of orange colour scattered light.
4, reach about 6cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination.
5, reach about 8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature drops to 7-11 ℃ rapidly in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 26-30 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 71-75% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 65-75 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.16-0.3%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 25-27 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 3.6-3.8%.
Embodiment ten:
1, carries out following technique improvement on the basis of the method for the conventional artificial greenhouse cultivation of employing glossy ganoderma and program.
2, the place is selected the higher highland that ventilates away from the pollution-free source in the signs of human habitation and roadside of height above sea level to build glossy ganoderma on the sunny side and is cultivated booth; Cultivate glossy ganoderma section wood and select after testing qualified hard section wood of maple; Get free of contamination clear water and cultivate relative humidity and the shower water of glossy ganoderma nurturing period in the booth as the regulation and control glossy ganoderma.
3, differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, or obtain the illumination of orange colour scattered light.
4, reach about 6.2cm at the glossy ganoderma bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma cultivation booth top shield net, can obtain black-and-blue scattered light illumination.
5, reach about 7.8cm at the glossy ganoderma bacteria cover diameter, every day at dusk the time-division glossy ganoderma is cultivated the booth ventilation opening and the door curtain that keeps out the wind opens wide, the regulation and control glossy ganoderma cultivates that room temperature drops to 8-12 ℃ rapidly in the booth; Early morning every day, glossy ganoderma was cultivated the booth ventilation opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 27-32 ℃ in the regulation and control glossy ganoderma booth on daytime; Simultaneously, it is 65-71% that regulation and control glossy ganoderma on daytime is cultivated the interior relative humidity of booth, and adopts common censer to light straw fumigation generation CO at every interval 72-80 minute
2Method, the regulation and control glossy ganoderma is cultivated in the booth and fills the air CO
2Concentration reaches 0.12-0.2%.
6, during the Reishi sporule of gathering, the regulation and control glossy ganoderma cultivates in the booth round the clock that room temperature is 24-25 ℃, and the fresh Reishi sporule that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained ganoderma lucidum triterpene compounds content of the Ganoderma spore of results is 3.2-3.4%.
Claims (4)
1. one kind promotes and induces glossy ganoderma secondary metabolite generation and the method that accumulates, differentiate cap glossy ganoderma and give environmental factors stimulation and regulation and control, comprise by regulation and control different light medium and light application time, difference variation, humidity variation and these environmental factorss of CO2 content stimulating the generation and the accumulation of inducing Ganoderma spore secondary metabolite ganoderma lucidum triterpene compounds with the growth and development stage that produces sporidium; The contained ganoderma lucidum triterpene compounds content of the Ganoderma spore that is obtained is up to 3.2-4.5%.
2. in accordance with the method for claim 1, it is characterized in that the concrete steps of this method are as follows:
(1), differentiate the growth and development stage of cap glossy ganoderma, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as glossy ganoderma, to obtain the illumination of orange colour scattered light;
(2), produce the growth and development stage of basidiospore glossy ganoderma, adopt the shield net of black-and-blue plastic rope wire material braiding to change glossy ganoderma and cultivate booth top shield net, to obtain black-and-blue scattered light illumination;
(3), in the Ganoderma sporophore stage of maturity, regulation and control glossy ganoderma at night cultivates that room temperature is 4-12 ℃ in the booth, daytime, glossy ganoderma cultivated that room temperature is 25-32 ℃ in the booth; Simultaneously, regulation and control glossy ganoderma on daytime cultivates that relative humidity is 65-75% in the booth, and the regulation and control glossy ganoderma is cultivated and fills the air CO2 concentration in the booth and reach 0.1-0.3%;
(4), the regulation and control glossy ganoderma of gathering during the Ganoderma spore cultivates in the booth round the clock that room temperature is 24-27 ℃.
3. in accordance with the method for claim 2, it is characterized in that prolonging light application time with orange light source bulb irradiation again in the step (1), intensity of illumination is the 1500-5000 Lux.
4. according to claim 2 or 3 described methods, it is characterized in that prolonging light application time with black-and-blue light source bulb irradiation again in the step (2), intensity of illumination is the 2000-6000 Lux.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| CN106962016A (en) * | 2016-01-14 | 2017-07-21 | 中国医学科学院药用植物研究所 | The method that ganoderma lucidum high yield and quality cultivation is realized using light |
| CN107691109A (en) * | 2017-11-28 | 2018-02-16 | 广西仙草堂制药有限责任公司 | A kind of cultural method of ecological ganoderma lucidum |
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2004
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| CN106962016A (en) * | 2016-01-14 | 2017-07-21 | 中国医学科学院药用植物研究所 | The method that ganoderma lucidum high yield and quality cultivation is realized using light |
| CN107691109A (en) * | 2017-11-28 | 2018-02-16 | 广西仙草堂制药有限责任公司 | A kind of cultural method of ecological ganoderma lucidum |
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