CN1273005C - Triterpene chemical production and accumulation method through inducing and promoting Ganoderma sinense Zhao - Google Patents
Triterpene chemical production and accumulation method through inducing and promoting Ganoderma sinense Zhao Download PDFInfo
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- CN1273005C CN1273005C CN 200410027684 CN200410027684A CN1273005C CN 1273005 C CN1273005 C CN 1273005C CN 200410027684 CN200410027684 CN 200410027684 CN 200410027684 A CN200410027684 A CN 200410027684A CN 1273005 C CN1273005 C CN 1273005C
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Abstract
The present invention relates to a method for promoting and inducing ganoderma sinense to generate and accumulate triterpene. In the developing growth period of the ganoderma sinense generating pileus and basidiospore, the ganoderma sinense is stimulated and regulated by environmental factors, such as different light quality and time, temperature change, CO2 content, etc., to stimulate and induce the ganoderma sinense spores to generate and accumulate secondary metabolite of triterpene. Analysis indicates that the triperpene content of obtained ganoderma sinense spores highly accounts for 3.1 to 4.6%. The obtained ganoderma sinense spores are further processed to be used as AIDS resistant medicines and radiotherapy and chemotherapy medicines and adjuvant treatment medicines of tumors to be applied to clinic.
Description
Technical field
The present invention relates to a kind ofly give at purple sesame growth and development stage that environmental factor stimulates and regulation and control, promote and induce the generation of purple sesame secondary metabolite triterpene compound and the method for accumulation.
Background technology
Purple sesame (Ganoderma Sinense) 1 year or perennial fungi are saprophytic bacteria, owing to can colonize on the tree that lives, so be called again facultative parasite.Carry " purple sesame can be controlled deafness, sharp joint, beneficial vital essence, hard muscles and bones ... " according to Shennong's Herbal.The successive dynasties physician all thinks purple sesame energy Zhiduo County kind disease has the nourishing and fit keeping function effect, energy brain tonic, anti-inflammatory, diuresis, beneficial stomach.Control diseases such as neurasthenia, dizzy insomnia, chronic hepatitis, pyelonephritis, bronchial astehma.Modern medicine makes substantial progress to effective component of glossy ganoderma analysis, pharmacology and clinical application research,, immunological regulation anti-ageing to glossy ganoderma, pharmacological action and mechanism of action antitumor and that activate blood circulation and disperse blood clots carry out big quantity research, particularly the purple Ganoderma lucidum spore of broken wall is applied to the clinical efficacy of tumor chemoradiotherapy supplemental treatment, enjoys Chinese scholars to attract attention.
In the last few years, finding more than 100 triterpene compound (Triterpene) in succession from the Ganoderma fungi, is the compounds with obvious physiologically active very important in the glossy ganoderma effective ingredient.Especially the pharmacologically active of triterpene compound in pharmaceutical research and clinical practice revealed after, in recent years to the research of purple sesame quality and quality and triterpene compound development of resources and separating-purifying subject study are become new study hotspot.
Summary of the invention
The purpose of this invention is to provide and a kind ofly give at purple sesame growth and development stage that environmental factor stimulates and regulation and control, promote and induce the generation of purple sesame secondary metabolite triterpene compound and the method for accumulation.
Discover; triterpene compound is that purple sesame process of growth is excited by environmental factor to induce to produce and the secondary metabolite of accumulation, makes to resist and to adapt to that its hereditary factor is stable not to morph and earn a bare living the protection material that organism was produced under extreme environment.The analysis showed that the purple sesame that grows under the natural environment contains triterpene compound and mainly is present in as in the purple Ganoderma lucidum spore of seed of going down to posterity, and is about 0.25-3.3% depending on quality discrepancy content; Then mainly concentrate on the periphery position of purple sesame cap in the fructification, and increase progressively along with the raising of its maturity; The mycelium that the employing deep layer is cultivated or extremely low with the contained triterpene compound content of the purple sesame of cultivation on sawdust.
Experiment of the present invention finds that purple sesame and purple Ganoderma lucidum spore quality that the domestication cultivates, the selection of culture base-material and nurturing an environment are crucial.If at purple sesame differential period oxygen deficiency, nurturing an environment CO
2Content surpasses 0.12%, and purple sesame cap stops to grow.As utilize the culture base-materials such as wood chip, corncob, bagasse to cultivate purple sesame, and though the purple sesame presentation of results and common purple sesame no significant difference, purple sesame cap quality pine is flat, and purple Ganoderma lucidum spore is shrivelled light, and the basidiospore germination rate is extremely low; As select hard section wood of broad leaf tree and good nurturing an environment to cultivate purple sesame, and the purple sesame cap stem quality of results is abundant, and toughness is strong, and purple Ganoderma lucidum spore is full to enrich contained triterpene compound content height, basidiospore germination rate height.
Method of the present invention is that the growth and development stage that differentiates cap and generation basidiospore purple sesame gives environmental factor stimulation and regulation and control, comprises by regulation and control different light medium and light application time, difference variation, humidity variation and CO
2These environmental factors of content stimulate generation and the accumulation of inducing purple Ganoderma lucidum spore secondary metabolite triterpene compound; The contained triterpene compound content of the purple Ganoderma lucidum spore that obtains is up to 3.1-4.6%.
The concrete steps of method of the present invention are as follows:
One, differentiates the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, to obtain the illumination of orange colour scattered light; Also can use the orange colour light source bulb irradiation prolonging light time, intensity of illumination is the 1000-4000 Lux again.
Two, producing the basidiospore stage purple sesame, generally is to reach about 7cm at purple sesame bacteria cover diameter, adopts the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, to obtain black-and-blue scattered light illumination; Also can be again with the black-and-blue light source bulb irradiation prolonging light time, intensity of illumination is the 1500-5000 Lux.
Three, in the purple sesame fructification stage of ripeness, generally be to reach about 10cm at purple sesame bacteria cover diameter, can purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind be opened wide in the dusk every day time-division, make ventilating convection promote purple sesame to cultivate room temperature rapid decrease in the booth, room temperature is 5-12 ℃ in the booth so that regulation and control purple sesame at night is cultivated; Can purple sesame cultivation booth ventilating opening and the door curtain lowering of keeping out the wind be closed in early morning every day, make room temperature rise in the purple sesame booth, so that room temperature is 26-33 ℃ in regulation and control purple sesame cultivation on the daytime booth; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 65-75%, and can produce CO by adopted common censer light the straw fumigation at every interval 60-80 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.15-0.3%.
Four, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 23-26 ℃.The new light violet Ganoderma lucidum spore that preferably will spray the same day was in time gathered at dusk and drying on the same day.The analysis showed that the contained triterpene compound content of the purple Ganoderma lucidum spore of results is up to 3.1-4.6%.
The inventive method is on the basis of the method for conventional artificial greenhouse cultivation glossy ganoderma, reaches purpose of the present invention by above-mentioned technique improvement.
According to the method for the invention described above, other specific procedure that purple sesame is cultivated such as the production of hybrid seeds of purple sesame bacterial classification, the wooden selection of section and detection, the stake inoculation of section wood fungus and send out that bacterium is cultivated, the place is selected and build purple sesame cultivate that booth, purple sesame cultivating and managing and purple Ganoderma lucidum spore gather etc. can be with reference to conventional method.
According to the method for the invention described above, the higher mountain region of height above sea level is selected preferably to utilize in the place, selects to build purple sesame away from the highland that ventilates in the pollution-free source in the signs of human habitation and roadside on the sunny side and cultivates booth, be convenient to round the clock between purple sesame cultivate and form the big temperature difference in the booth; Preferably select the wooden hard section wood such as broad leaf tree such as maple, rice sweet oak, evergreen chinquapin, robur, plum to cultivate purple sesame, and the wooden middle heavy metal of sampling detection segment wood and agricultural residual content, the Duan Mu that strict control is selected meets common cultivation organic culture medium of edible fungus material heavy metal and agricultural residual content's index; Control is used for regulating the purple interior relative humidity of sesame cultivation booth and the shower water of purple sesame nurturing period is selected free of contamination mountain spring water or clear water.
Test of the present invention shows that different light medium and light application time, difference variation and humidity change, CO
2The environmental factors such as content are to stimulating purple sesame to grow and the triterpene compound changes of contents presents otherness.Differentiate the cap stage purple sesame, the illumination of orange colour scattered light and prolonging light time are provided, can promote purple sesame cap to thicken, produce the stage of basidiospore purple sesame, changing light quality is black-and-blue scattered light and prolonging light time, can promote purple sesame to produce a large amount of basidiospore; Room temperature is 23-26 ℃ in the purple sesame cultivation of the regulation and control booth during the purple Ganoderma lucidum spore of gathering, and it is vigorous to promote that purple Ganoderma lucidum spore sprays; In the purple sesame fructification stage of ripeness, regulate and control in the purple sesame nurturing an environment round the clock between temperature difference amplitude, humidity and CO
2Content also forms extreme environment, can stimulate generation and the accumulation of inducing purple Ganoderma lucidum spore secondary metabolite triterpene compound, and purple Ganoderma lucidum spore quality significantly improves, and triterpene compound content is up to 3.1-4.6% in the purple Ganoderma lucidum spore.Through further processing, can be used as anti-AIDS and tumor chemoradiotherapy medicine and adjuvant therapy medicaments and be applied to clinical.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3000 Lux.
4, reach about 6.9cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3500-4000 Lux.
5, reach about 10cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 5-7 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 26-29 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 65-70%, and adopts common censer to light straw fumigation generation CO at every interval 60-65 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.15-0.2%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 23-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.3-4.5%.
Embodiment two:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source in the signs of human habitation and roadside of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination clear water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light.
4, reach about 7cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination.
5, reach about 10cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature rapid decrease of purple sesame cultivation booth is 10-12 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 30-32 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 70-75%, and adopts common censer to light straw fumigation generation CO at every interval 75-80 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.2-0.3%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 25-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 3.3-3.4%.
Embodiment three:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 1000-2500 Lux.
4, reach about 7cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3500-4200 Lux.
5, reach about 9.8cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 5-8 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 26-29 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 70-72%, and adopts common censer to light straw fumigation generation CO at every interval 65-70 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.2-0.25%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 23-25 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.0-4.2%.
Embodiment four:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3000 Lux.
4, reach about 7cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 1500-2800 Lux.
5, reach about 10.2cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 8-10 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 29-33 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 72-74%, and adopts common censer to light straw fumigation generation CO at every interval 60-65 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.25-0.3%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 24-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.2-4.3%.
Embodiment five:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3000 Lux.
4, reach about 6.8cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination.Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3500 Lux.
5, reach about 9.8cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 6-7 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 29-32 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 72-74%, and adopts common censer to light straw fumigation generation CO at every interval 62-75 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.15-0.25%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 23-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.2-4.3%.
Embodiment six:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of Japanese apricot; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 1500-2500 Lux.
4, reach about 7cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 2500-3500 Lux.
5, reach about 10cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 7-8 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 26-31 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 70-75%, and adopts common censer to light straw fumigation generation CO at every interval 68-75 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.2-0.25%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 24-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.2-4.4%.
Embodiment seven:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2700-3500 Lux.
4, reach about 7cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 3500-4800 Lux.
5, reach about 10cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 5-9 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 31-32 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 69-75%, and adopts common censer to light straw fumigation generation CO at every interval 68-76 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.2-0.25%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 24-25 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.4-4.5%.
Embodiment eight:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of rice sweet oak tree; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light; Adopt the orange colour light source bulb irradiation prolonging light time night, intensity of illumination is the 2000-3500 Lux.
4, reach about 6.9cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination; Adopt the black-and-blue light source bulb irradiation prolonging light time night, intensity of illumination is the 2500-4500 Lux.
5, reach about 10.2cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature of purple sesame cultivation booth is 5-7 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 30-32 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 68-70%, and adopts common censer to light straw fumigation generation CO at every interval 60-65 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.15-0.25%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 25-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 4.4-4.6%.
Embodiment nine:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source in the signs of human habitation and roadside of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of Japanese apricot; Get free of contamination clear water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, can obtain the illumination of orange colour scattered light.
4, reach about 7.1cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination.
5, reach about 10.2cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature rapid decrease of purple sesame cultivation booth is 7-8 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 29-32 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 70-75%, and adopts common censer to light straw fumigation generation CO at every interval 70-75 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.16-0.3%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 24-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 3.3-3.6%.
Embodiment ten:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source in the signs of human habitation and roadside of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination clear water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, or obtain the illumination of orange colour scattered light.
4, reach about 7.1cm at purple sesame bacteria cover diameter, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame cultivation booth top shield net, can obtain black-and-blue scattered light illumination.
5, reach about 9.8cm at purple sesame bacteria cover diameter, in purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide the dusk every day time-division, regulating and control the interior room temperature rapid decrease of purple sesame cultivation booth is 8-12 ℃; Early morning every day, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and room temperature is 31-33 ℃ in the regulation and control purple sesame booth on daytime; Simultaneously, regulating the interior relative humidity of purple sesame cultivation on daytime booth is 65-68%, and adopts common censer to light straw fumigation generation CO at every interval 75-80 minute
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.21-0.3%.
6, during the purple Ganoderma lucidum spore of gathering, to regulate and control purple sesame and cultivate in the booth round the clock that room temperature is 23-26 ℃, the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the purple Ganoderma lucidum spore of results is 3.1-3.5%.
Claims (3)
1. one kind promotes and induces purple sesame to produce and accumulate the method for triterpene compound, it is characterized in that the concrete steps of this method are as follows:
(1), select for use hard section wood of broad-leaved tree to cultivate purple sesame;
(2), differentiate the cap stage purple sesame, adopt the shield net of orange colour plastic rope wire material braiding to cultivate booth top shield net as purple sesame, to obtain the illumination of orange colour scattered light;
(3), when purple sesame bacteria cover diameter reaches 6.8-7.1cm, adopt the shield net of black-and-blue plastic rope wire material braiding to change purple sesame and cultivate booth top shield net, to obtain black-and-blue scattered light illumination;
(4), when purple sesame bacteria cover diameter reaches 9.8-10.2cm, regulation and control purple sesame at night cultivates that room temperature is 5-12 ℃ in the booth, daytime, purple sesame cultivated that room temperature is 26-33 ℃ in the booth; Simultaneously, regulating the interior relative moisture of purple sesame cultivation on daytime booth is 65-75%, regulates and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.15-0.3%;
(5), the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 23-26 ℃.
2. in accordance with the method for claim 1, it is characterized in that step (2) adopts orange colour light source bulb irradiation to prolong light application time middle night, intensity of illumination is the 1000-4000 lux.
3. according to claim 1 or 2 described methods, it is characterized in that step (3) adopts black-and-blue light source bulb irradiation to prolong light application time middle night, intensity of illumination is the 1500-5000 lux.
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