CN1883248A - Method for improving quality of Ganoderma lucidium spore - Google Patents
Method for improving quality of Ganoderma lucidium spore Download PDFInfo
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- CN1883248A CN1883248A CN 200610036124 CN200610036124A CN1883248A CN 1883248 A CN1883248 A CN 1883248A CN 200610036124 CN200610036124 CN 200610036124 CN 200610036124 A CN200610036124 A CN 200610036124A CN 1883248 A CN1883248 A CN 1883248A
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Abstract
The invention relates to a method for quality promotion of ganoderma lucidum spores during different growth and development stages of ganoderma lucidum. The method comprises cultivating ganoderma lucidums with rigid segment wood of broad leaf trees, spraying tea leach during the growth and development stage from pilei differentiation to basidiospore production; regulating temperature in a ganoderma lucidum cultivation booth, to enable that the night temperature of is 12-32 DEG C lower than the day temperature, during the growth and development stage from ganoderma lucidum pileus diameter reaching 6cm to before basidiospore production; regulating CO2 concentration to 0.10-0.20% in the ganoderma lucidum cultivation booth during basidiospore production stage; and regulating temperature of day and night to 25-30DEG C in the ganoderma lucidum cultivation booth during ganoderma lucidum spore collection period. Ganoderma lucidum spores have up to 3.5-4.3% by wt of triterpene and up to 36.8-37.8 % by wt of lipid active composition, detected by a standard chemical analysis method. Ganoderma lucidum spores can be used as cilincal auxiliary treatment drug for AIDS and cancer after further processing.
Description
Technical field
The present invention relates to the method that a kind of different phase of growing red sesame promotes and promote red Ganoderma lucidum spore quality.
Background technology
About the record of red sesame (Ganoderma lucidum) can be traced back to B.C. 300 years " Book of Rites " and " Springs and Autumns of Master L ".More detailed description then appears at the pharmacopeia Compendium of Material Medica of the B.C. 100 years Shennong's Herbal and the Ming Dynasty about red sesame effect.In China and other oriental countries, it is considered to have one of best folk medicine of promoting healthy and the effect of promoting longevity and is used and enjoy high praise for a long time.Red sesame is a kind of medicinal fungi that contains multiple bioactivator.The successive dynasties physician thinks that all red sesame can plant disease in Zhiduo County, and the nourishing and fit keeping function effect is arranged, though red sesame fruit body be used as medicine in the application history in existing several thousand of China, until 20th century red Ganoderma lucidum spore just be familiar with and utilize by people.And be widely used in brain tonic, anti-inflammatory, diuresis, beneficial stomach and control diseases such as neurasthenia, dizzy insomnia, chronic hepatitis, pyelonephritis, bronchial astehma.The existing many patents of scientific payoffs and the scientific literature report of the clinical testing of the red Ganoderma lucidum spore treatment of chronic diseases of relevant application and pharmaceutical research.
In recent years, finding more than 100 triterpene compound (Triterpene) in succession from the Ganoderma fungi, is the compounds with obvious physiologically active very important in the glossy ganoderma effective ingredient.After the pharmacologically active of lipid active ingredients such as triterpene compound in the tumor aid treatment clinical practice is revealed, to how improving red sesame and red Ganoderma lucidum spore quality, and promote lipid active ingredient such as triterpene compound to become new research focus in the pharmacology synergistic effect research of clinical practice.It is reported, the tealeaves leaching liquor has good synergistic effect in fungus secondary metabolite such as antibiotic clinical practice, wherein tealeaves Main Ingredients and Appearance Tea Polyphenols, caffeine, catechin are scientist's popular research topics to the research of pharmacology synergistic effect mechanism.
Summary of the invention
The purpose of this invention is to provide a kind of different phase of growing, promote and promote the method for red Ganoderma lucidum spore quality red sesame.
Ganoderma lucidum triterpene compounds isoreactivity composition is that red sesame growth and development process is induced by environmental factor to produce and the secondary metabolite of accumulation, is to adapt to and resist the protection material that the organism normal development that earns a bare living under the extreme environment is produced.Experiment of the present invention is found, utilize hard section wood of broad-leaved tree to cultivate red sesame, spray an amount of tealeaves leaching liquor at its growth and development stage, can make in the composition of red Ganoderma lucidum spore of results and contain the catechin and the caffeine of trace, and can effectively promote the activity of red sesame polyphenol oxidase and promote the activity of lipid active ingredients such as triterpene compound; Simultaneously, by different phase growing environment regulation and control day and night temperature and employing CO that red sesame is grown
2Stimulate, the red sesame of being cultivated, its cap stem quality is abundant, lipid active ingredient content height such as the interior triterpene compound of red Ganoderma lucidum spore.Evident in efficacy in the clinical research that is applied to acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments or cancer adjuvant therapy medicament.Find that in pharmacological experiment the inventive method is cultivated micro-catechin contained in the red Ganoderma lucidum spore of results and caffeine the activity of lipid active ingredients such as triterpene compound is had significant pharmacology synergistic effect.
Method of the present invention is: utilize hard section wood of broad-leaved tree to cultivate red sesame, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, spray the tealeaves leaching liquor; Reach more than the 6cm growth and development stage before produce basidiospore at red sesame bacteria cover diameter, regulate and control red sesame and cultivate day and night temperature in the booth, make the night temperature be lower than 12～32 ℃ of daytimes; Produce the basidiospore stage red sesame, adopt CO
2Stimulate, regulate and control red sesame and cultivate CO in the booth
2Concentration reaches 0.10-0.20%; The red sesame of regulation and control of gathering during the red Ganoderma lucidum spore is cultivated in the booth round the clock, and room temperature is 25-30 ℃.Red sesame and the red Ganoderma lucidum spore cultivated are best in quality, and the red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and red Ganoderma lucidum spore triterpene compound content is up to 3.5-4.3wt.%, and lipid active ingredient content is up to 36.8-37.8wt.%.
The used tealeaves leaching liquor of the inventive method can be obtained by following method: with tealeaves and water in tealeaves: water=1: the ratio of 25-50 weight ratio at 80-100 ℃ of following lixiviate 5-30 minute, obtains required tealeaves leaching liquor; Used local tea variety can be one or more in black tea, green tea, blue or green tea, the Oolong tea.
The concrete steps of method of the present invention are as follows:
One, utilize hard section wood of broad-leaved tree to cultivate red sesame.
Two, grow red sesame and differentiate cap to the growth and development stage that produces basidiospore, spray the tealeaves leaching liquor respectively once in morning every day, noon, evening, the amount of spraying is every square metre and sprays 30-55g at every turn.
Three, differentiate cap in red sesame growth, red sesame bacteria cover diameter reaches 6-7.5cm to producing basidiospore growth and development stage before, can at dusk the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind be opened wide in every day, make ventilating convection promote red sesame to cultivate the interior room temperature of booth and descend rapidly, room temperature is 3-15 ℃ in the booth so that regulation and control red sesame at night is cultivated; Can the red sesame cultivation booth ventilating opening and the door curtain lowering of keeping out the wind be closed in every morning, make room temperature rise in the red sesame booth, be 27-35 ℃ so that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Simultaneously, regulating the interior relative moisture of red sesame cultivation on daytime booth is 85-95%.
Four, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered,, can produce CO by adopted common censer light the straw fumigation in 180～220 minutes at every interval on the daytime of every day (beginning morning) at dusk
2Method, regulate and control red sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.10-0.20%.
Five, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25-30 ℃.Preferably the fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.
The inventive method is on the basis of the method for conventional artificial greenhouse cultivation glossy ganoderma, reaches purpose of the present invention by above-mentioned technique improvement.
According to the method for the invention described above, other specific procedure that red sesame is cultivated such as the production of hybrid seeds of red sesame bacterial classification, the wooden selection of section and detection, the stake inoculation of section wood fungus and send out that bacterium is cultivated, the place is selected and build red sesame cultivate that booth, red sesame cultivating and managing and red Ganoderma lucidum spore gather etc. can be with reference to conventional method.
According to the method for the invention described above, the higher mountain region of height above sea level is selected preferably to utilize in the place, selects to build red sesame away from the highland that ventilates in the pollution-free source in the signs of human habitation and roadside on the sunny side and cultivates booth, be convenient to round the clock between red sesame cultivate and form the big temperature difference in the booth; Preferably select for use wooden hard section wood such as broad-leaved tree such as maple, rice sweet oak, evergreen chinquapin, robur or Japanese apricot to cultivate red sesame, and wooden middle heavy metal of sampling detection segment wood and agricultural residual content, the Duan Mu that strict control is selected for use meets common cultivation organic culture medium of edible fungus material heavy metal and agricultural residual content's index; Control is used to regulate the red interior relative moisture of sesame cultivation booth and the shower water of red sesame nurturing period is selected free of contamination mountain spring water or clear water for use.
It is best in quality to adopt the inventive method to cultivate red sesame and the red Ganoderma lucidum spore gathered in the crops, and red sesame cap stem quality is abundant, and red Ganoderma lucidum spore is full full; Chemical analysis method through adopting standard detects, and lipid active ingredient content such as the interior triterpene compound of red Ganoderma lucidum spore are higher, and its triterpene compound content is up to 3.5-4.3wt.%, and lipid active ingredient content is up to 36.8-37.8wt.%.The red sesame of results and red Ganoderma lucidum spore can be used as acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments or cancer adjuvant therapy medicament and are applied to clinical through further processing.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the black tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 50g at every turn, and the concentration of black tea leaching liquor is black tea and 1: 42 weight ratio of water ratio; The extraction temperature of lixiviate black tea is 96 ℃, and extraction time is 5 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 7cm, 6: 30 at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 10 ℃ in regulation and control red sesame cultivation at the night booth; 7 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 27 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 95%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, 7: 30 extremely at dusk 6: 10 every morning, the 180 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.13%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 28 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 4.3wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 37.6wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Embodiment two:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of rice sweet oak; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the black tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 40g at every turn, and the concentration of green tea leaching liquor is green tea and 1: 30 weight ratio of water ratio; The extraction temperature of lixiviate green tea is 98 ℃, and extraction time is 25 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6.5cm, 6 o'clock at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 15 ℃ in regulation and control red sesame cultivation at the night booth; 7 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 29 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 85%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, 6: 35 extremely at dusk 7: 40 every morning, the 220 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.2%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered and drying before the big dusk.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 3.5wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 36.8wt.%.Through further processing, can be used as cancer adjuvant therapy medicament and be applied to clinical.
Embodiment three:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed blue or green tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 25g at every turn, and the concentration of blue or green tea leaching liquor is blue or green tea and 1: 25 weight ratio of water ratio; The extraction temperature of the blue or green tea of lixiviate is 95 ℃, and extraction time is 30 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 7.5cm, 6: 30 at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 3 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 35 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 95%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, 7 o'clock extremely at dusk 8: 30 every morning, the 180 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.11%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 3.7wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 37.8wt.%.Through further processing, can be used as the immune response medicinal application in clinical.
Embodiment four:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of Japanese apricot; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the Oolong tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 40g at every turn, and the concentration of Oolong tea leaching liquor is Oolong tea and 1: 38 weight ratio of water ratio; The extraction temperature of lixiviate Oolong tea is 92 ℃, and extraction time is 15 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6.5cm, 6 o'clock at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 15 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 28 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 95%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, 7 o'clock extremely at dusk 8: 30 every morning, the 180 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.1%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 29 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 3.6wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 36.8wt.%.Through further processing, can be used as acquired immune deficiency syndrome (AIDS) or cancer adjuvant therapy medicament and be applied to clinical.
Embodiment five:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed leaching liquor that blue or green tea and green tea mixes at 7 o'clock at dusk once, the amount of spraying is every square metre and sprays 45g at every turn, and the concentration of blue or green tea and green tea leaching liquor is tea and 1: 40 weight ratio of water ratio; The ratio of blue or green tea and green tea 1: 1; The blue or green tea of lixiviate and green tea extraction temperature are 80 ℃, and extraction time is 15 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6cm, 6 o'clock at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 4 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 27 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 90%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, 6: 30 extremely at dusk 8: 30 every morning, the 190 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.15%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 28 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 4wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 37wt.%.Through further processing, can be used as AIDS-treating medicine and be applied to clinical.
Embodiment six:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of rice sweet oak tree; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the green tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 47g at every turn, and the concentration of green tea leaching liquor is green tea and 1: 30 weight ratio of water ratio; The extraction temperature of lixiviate green tea is 100 ℃, and extraction time is 22 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6cm, 6: 30 at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 8 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 32 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 85%.
5, before red sesame produces basidiospore stage to red Ganoderma lucidum spore and gathers, every morning 8 up to 6 o'clock at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.10%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 4.0wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 36.9wt.%.Through further processing, can be used as cancer adjuvant therapy medicament and be applied to clinical.
Embodiment seven:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of Japanese apricot; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed blue or green tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 30g at every turn, and the concentration of blue or green tea leaching liquor is blue or green tea and 1: 33 weight ratio of water ratio; The extraction temperature of the blue or green tea of lixiviate is 92 ℃, and extraction time is 15 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6.5cm, 6: 30 at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 16 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 32 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 90%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, in 8: 30 every morning of 6: 30 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.15%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 26 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 3.8wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 36.9wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Embodiment eight:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed blue or green tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 50g at every turn, and the concentration of blue or green tea leaching liquor is blue or green tea and 1: 35 weight ratio of water ratio; The extraction temperature of the blue or green tea of lixiviate is 96 ℃, and extraction time is 20 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6.5cm, 6 o'clock at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 4 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 34 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 90%.
5, before red sesame produces basidiospore stage to red Ganoderma lucidum spore and gathers, every morning 8 up to 7 o'clock at dusk, the 200 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.12%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 30 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 4.1wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 37.2wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Embodiment nine:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the black tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 45g at every turn, and the concentration of black tea leaching liquor is black tea and 1: 38 weight ratio of water ratio; The extraction temperature of lixiviate black tea is 92 ℃, and extraction time is 18 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6.3cm, 6: 30 at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 6 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 27 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 95%.
5, before red sesame produces basidiospore stage to red Ganoderma lucidum spore and gathers, every morning 8 up to 6 o'clock at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.13%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 26 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 4.3wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 37.7wt.%.Through further processing, can be used as cancer adjuvant therapy medicament and be applied to clinical.
Embodiment ten:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for red sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build red sesame on the sunny side and is cultivated booth; Cultivate red sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of red sesame nurturing period in the booth as the red sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in red sesame growth, 7 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the Oolong tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 35g at every turn, and the concentration of Oolong tea leaching liquor is Oolong tea and 1: 46 weight ratio of water ratio; The extraction temperature of lixiviate Oolong tea is 100 ℃, and extraction time is 5 minutes.
4, differentiate cap in red sesame growth, when red sesame bacteria cover diameter reaches 6.5cm, 6: 30 at dusk every day the red sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, room temperature is 4 ℃ in regulation and control red sesame cultivation at the night booth; 8 o'clock every mornings, red sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 34 ℃ that regulation and control red sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of red sesame cultivation on daytime booth is 85%.
5, before the red sesame generation basidiospore stage, extremely red Ganoderma lucidum spore was gathered, in 8: 30 every morning of 6: 30 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the red sesame cultivation booth to fill the air CO
2Concentration reaches 0.20%.
6, the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The fresh red Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the red sesame and the red Ganoderma lucidum spore of results are best in quality, and red sesame cap stem quality is abundant.The red Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 3.9wt.% in the red Ganoderma lucidum spore, and the lipid activity substance content is up to 37.2wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Claims (4)
1. a method that promotes and promote red Ganoderma lucidum spore quality utilizes hard section wood of broad-leaved tree to cultivate red sesame, grows red sesame to differentiate cap until the growth and development stage that produces basidiospore, sprays the tealeaves leaching liquor; Reach more than the 6cm growth and development stage before produce basidiospore at red sesame bacteria cover diameter, regulate and control red sesame and cultivate day and night temperature in the booth, make the night temperature be lower than 12～32 ℃ of daytimes; Produce the basidiospore stage red sesame, adopt CO
2Stimulate, regulate and control red sesame and cultivate CO in the booth
2Concentration reaches 0.10-0.20%; The red sesame of regulation and control of gathering during the red Ganoderma lucidum spore is cultivated in the booth round the clock, and room temperature is 25-30 ℃.
2. in accordance with the method for claim 1, it is characterized in that described tealeaves leaching liquor is obtained by following method: with tealeaves and water in tealeaves: water=1: the ratio of 25-50 weight ratio, at 80-100 ℃ of following lixiviate 5-30 minute, obtain required tealeaves leaching liquor; Used local tea variety is one or more in black tea, green tea, blue or green tea, the Oolong tea.
3. according to claim 1 or 2 described methods, it is characterized in that the concrete steps of this method are as follows:
(1), cultivates red sesame with hard section wood of broad-leaved tree;
(2), grow red sesame and to differentiate cap until the growth and development stage that produces basidiospore, spray the tealeaves leaching liquor respectively once in morning every day, noon, evening, the amount of spraying is every square metre and sprays 30-55g at every turn;
(3), reach the growth and development stage of 6-7.5cm before produce basidiospore, regulation and control red sesame at night cultivates that room temperature is 3-15 ℃ in the booth, and regulation and control red sesame on daytime cultivates that room temperature is 27-35 ℃ in the booth at red sesame bacteria cover diameter; Simultaneously, regulating the interior relative moisture of red sesame cultivation on daytime booth is 85-95%;
(4), before red sesame produces basidiospore stage to red Ganoderma lucidum spore and gathers, begin at dusk in every morning, every interval was adopted common censer to light the straw fumigation in 180～220 minutes and is produced CO
2Method, regulate and control red sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.10-0.20%;
(5), the red sesame of regulation and control of gathering during the red Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25-30 ℃.
4. according to the described method of claim 1,2 or 3, it is characterized in that hard section wood of described broad-leaved tree comprises the wooden hard Duan Mu of maple, rice sweet oak, evergreen chinquapin, robur or Japanese apricot.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550296A (en) * | 2012-02-15 | 2012-07-11 | 梅州市微生物研究所 | Indoor cultivation method for Ganoderma lucidum |
| CN105409596A (en) * | 2015-12-21 | 2016-03-23 | 广东中源农业发展有限公司 | Ganoderma lucidum substitute cultivation method |
| CN110089340A (en) * | 2018-01-31 | 2019-08-06 | 蒋凡 | A kind of method of castanopsis fissa underwood planting ganoderma lucidum |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85100417B (en) * | 1985-04-01 | 1988-02-17 | 山东省枣庄市薛城真菌实验站 | Greenhouse cultivation of ganoderma lucidum |
| CN1243092C (en) * | 2003-09-28 | 2006-02-22 | 吴佛煌 | Liquid nutrient medium for artificial cultural ganoderma lucidum and cultivation method |
-
2006
- 2006-06-28 CN CNB2006100361240A patent/CN100433966C/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550296A (en) * | 2012-02-15 | 2012-07-11 | 梅州市微生物研究所 | Indoor cultivation method for Ganoderma lucidum |
| CN105409596A (en) * | 2015-12-21 | 2016-03-23 | 广东中源农业发展有限公司 | Ganoderma lucidum substitute cultivation method |
| CN110089340A (en) * | 2018-01-31 | 2019-08-06 | 蒋凡 | A kind of method of castanopsis fissa underwood planting ganoderma lucidum |
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