CN100499994C - Method for improving the quality of violet glossy ganoderma and its spore - Google Patents
Method for improving the quality of violet glossy ganoderma and its spore Download PDFInfo
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- CN100499994C CN100499994C CNB2006100361236A CN200610036123A CN100499994C CN 100499994 C CN100499994 C CN 100499994C CN B2006100361236 A CNB2006100361236 A CN B2006100361236A CN 200610036123 A CN200610036123 A CN 200610036123A CN 100499994 C CN100499994 C CN 100499994C
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Abstract
A method for improving the quality of violet gill fungus and its spores includes such steps as culturing on the hard wood of broad-leaf tree, spraying liquid extract of tea in the stage from differentiating cap to generate basidiospores, regulating the temp difference between day and night to 13-32 deg.C when the diameter of its cap reaches more than 5 cm, regulating the concentration of CO2 to 0.01-0.15% before the spores are harvested, and regulating the temp difference between day and night to 25-28 deg.C when the spores are harvesting.
Description
Technical field
The present invention relates to the method that a kind of different phase of growing purple sesame promotes and promote purple sesame and purple Ganoderma lucidum spore quality.
Background technology
Can trace back to the pharmacopeia Compendium of Material Medica of the B.C. 100 years Shennong's Herbal and the Ming Dynasty about the detailed description of purple sesame (Ganoderma Sinense) effect.Shennong's Herbal carries, " purple sesame can be controlled deafness, sharp joint, beneficial vital essence, hard muscles and bones ... ".The successive dynasties physician all thinks purple sesame energy Zhiduo County kind disease has the nourishing and fit keeping function effect, energy brain tonic, anti-inflammatory, diuresis, beneficial stomach.Control diseases such as neurasthenia, dizzy insomnia, chronic hepatitis, pyelonephritis, bronchial astehma.Modern medicine makes substantial progress to the analysis of glossy ganoderma effective ingredient, pharmacology and clinical application research,, immunological regulation anti-ageing to glossy ganoderma, pharmacological action and mechanism of action antitumor and that activate blood circulation and disperse blood clots carry out big quantity research, particularly the purple Ganoderma lucidum spore of broken wall is applied to the clinical efficacy of tumor chemoradiotherapy supplemental treatment, enjoys Chinese scholars to attract attention.
In recent years, finding more than 100 triterpene compound (Triterpene) in succession from the Ganoderma fungi, is the compounds with obvious physiologically active very important in the glossy ganoderma effective ingredient.After the pharmacologically active of lipid active ingredients such as triterpene compound in immune response and tumor aid treatment clinical practice is revealed, to how improving purple sesame and purple Ganoderma lucidum spore quality, and promote lipid active ingredient pharmacology synergistic effect research clinically such as triterpene compound to become new research focus.It is reported, the tealeaves leaching liquor has good synergistic effect in fungus secondary metabolite such as antibiotic clinical practice, wherein the research of the pharmacology synergistic effect mechanism of tealeaves Main Ingredients and Appearance Tea Polyphenols, caffeine, catechin is scientist's popular research topic.
Summary of the invention
The purpose of this invention is to provide a kind of different phase of growing, promote and promote the method for purple sesame and purple Ganoderma lucidum spore quality purple sesame.
Ganoderma lucidum triterpene compounds isoreactivity composition is that purple sesame growth and development process is induced by environmental factor to produce and the secondary metabolite of accumulation, is to adapt to and resist the protection material that the organism normal development that earns a bare living under the extreme environment is produced.Experiment of the present invention is found, utilize hard section wood of broad-leaved tree to cultivate purple sesame, spray an amount of tealeaves leaching liquor in its stage of growing, can make in the composition of the purple sesame of results and purple Ganoderma lucidum spore and contain the catechin and the caffeine of trace, and can effectively promote the activity of purple sesame polyphenol oxidase and promote the activity of lipid active ingredients such as triterpene compound; Simultaneously, stimulate by purple sesame is grown different phase growing environment regulation and control day and night temperature and employing CO2, the purple sesame of being cultivated, its cap stem quality is abundant, lipid active ingredient content height such as the interior triterpene compound of purple Ganoderma lucidum spore.Be applied to acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments or cancer adjuvant therapy medicament or be applied in the clinical research of immune response medicine evident in efficacy.Find that in pharmacological experiment the inventive method is cultivated micro-catechin contained in the purple sesame of results and the purple Ganoderma lucidum spore and caffeine the activity of lipid active ingredients such as triterpene compound is had significant pharmacology synergistic effect.
Method of the present invention is: cultivate purple sesame with hard section wood of broad-leaved tree; Differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, spray an amount of tealeaves leaching liquor; Reach more than the 5cm growth and development stage before produce basidiospore at purple sesame bacteria cover diameter, regulate and control purple sesame and cultivate day and night temperature in the booth, make the night temperature be lower than 13～32 ℃ of daytimes; Before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, adopt CO
2Stimulate, regulate and control purple sesame and cultivate CO in the booth
2Concentration reaches 0.10-0.15%; The purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore is cultivated in the booth round the clock, and room temperature is 25-28 ℃.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and purple Ganoderma lucidum spore triterpene compound content is up to 3.2-4.85wt.%, and lipid active ingredient content is up to 35-40.9wt.%.
The used tealeaves leaching liquor of the inventive method can be obtained by following method: with tealeaves and water in tealeaves: water=1: the ratio of 30-40 weight ratio at 90-100 ℃ of following lixiviate 5-20 minute, obtains required tealeaves leaching liquor; Used local tea variety can be one or more in black tea, green tea, blue or green tea, the Oolong tea.
Method of the present invention can be undertaken by following concrete steps:
One, select for use hard section wood of broad-leaved tree to cultivate purple sesame.
Two, grow purple sesame and differentiate cap to the growth and development stage that produces basidiospore, spray the tealeaves leaching liquor respectively once in morning every day, noon, evening, the amount of spraying is every square metre and sprays 20-50g at every turn.
Three, differentiate cap in purple sesame growth, purple sesame bacteria cover diameter reaches 5～8.5cm to producing basidiospore growth and development stage before, can at dusk the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind be opened wide in every day, make ventilating convection promote purple sesame to cultivate the interior room temperature of booth and descend rapidly, room temperature is 3-14 ℃ in the booth so that regulation and control purple sesame at night is cultivated; Can the purple sesame cultivation booth ventilating opening and the door curtain lowering of keeping out the wind be closed in every morning, make room temperature rise in the purple sesame booth, so that room temperature is 27-35 ℃ in regulation and control purple sesame cultivation on the daytime booth; Simultaneously, regulating the interior relative moisture of purple sesame cultivation on daytime booth is 85-95%.
Four, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered,, can produce CO by adopted common censer light the straw fumigation in 180～240 minutes at every interval on the daytime of every day (beginning morning) at dusk
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.10-0.15%.
Five, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25-28 ℃.Preferably the new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.
The inventive method is on the basis of the method for conventional artificial greenhouse cultivation glossy ganoderma, reaches purpose of the present invention by above-mentioned technique improvement.
According to the method for the invention described above, other specific procedure that purple sesame is cultivated such as the production of hybrid seeds of purple sesame bacterial classification, the wooden selection of section and detection, the stake inoculation of section wood fungus and send out that bacterium is cultivated, the place is selected and build purple sesame cultivate that booth, purple sesame cultivating and managing and purple Ganoderma lucidum spore gather etc. can be with reference to conventional method.
According to the method for the invention described above, the higher mountain region of height above sea level is selected preferably to utilize in the place, selects to build purple sesame away from the highland that ventilates in the pollution-free source in the signs of human habitation and roadside on the sunny side and cultivates booth, be convenient to round the clock between purple sesame cultivate and form the big temperature difference in the booth; Preferably select for use wooden hard section wood such as broad-leaved tree such as maple, rice sweet oak, evergreen chinquapin, robur or Japanese apricot to cultivate purple sesame, and wooden middle heavy metal of sampling detection segment wood and agricultural residual content, the Duan Mu that strict control is selected for use meets common cultivation organic culture medium of edible fungus material heavy metal and agricultural residual content's index; Control is used to regulate the purple interior relative moisture of sesame cultivation booth and the shower water of purple sesame nurturing period is selected free of contamination mountain spring water or clear water for use.
The purple sesame cap stem quality that the inventive method is cultivated is abundant, and the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple Ganoderma lucidum spore is full full; Chemical analysis method through adopting standard detects, lipid active ingredient content height such as the interior triterpene compound of purple Ganoderma lucidum spore, and its triterpene compound content is up to 3.2-4.85wt.%, and the lipid activity substance content is up to 35-40.9wt.%.The purple sesame of results and purple Ganoderma lucidum spore can be used as acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments or cancer adjuvant therapy medicament or immune response medicinal application in clinical through further processing.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the black tea leaching liquor once at 8 o'clock at dusk, the amount of spraying is every square metre and sprays 30g at every turn, and the concentration of black tea leaching liquor is black tea and water ratio 1:40 weight ratio; The extraction temperature of lixiviate black tea is 95 ℃, and extraction time is 5 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 6cm, about 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 5 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; About 8 o'clock every mornings the purple sesame cultivation booth ventilating opening and the door curtain lowering of keeping out the wind are closed, it is 30 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 90%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, 6: 30 extremely at dusk 8: 30 every morning, the 180 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.13%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 28 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and triterpene compound content is up to 4.85wt.% in the purple Ganoderma lucidum spore, and the lipid activity substance content is up to 38.6wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Embodiment two:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of rice sweet oak; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the black tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 50g at every turn, and the concentration of green tea leaching liquor is green tea and water ratio 1:30 weight ratio; The extraction temperature of lixiviate green tea is 100 ℃, and extraction time is 20 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 7cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 14 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 32 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 95%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 40 every morning of 6: 35 timesharing extremely at dusk, the 240 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.15%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and the triterpene compound component content is up to 4.7wt.% in the purple Ganoderma lucidum spore, and the lipid activity substance content is up to 36.5wt.%.Through further processing, can be used as cancer adjuvant therapy medicament and be applied to clinical.
Embodiment three:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of evergreen chinquapin; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 7 o'clock every mornings, at 13 o'clock at noon, respectively sprayed blue or green tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 25g at every turn, and the concentration of blue or green tea leaching liquor is blue or green tea and water ratio 1:35 weight ratio; The extraction temperature of the blue or green tea of lixiviate is 90 ℃, and extraction time is 10 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 7.5cm, 7: 30 at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 8 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 33 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 88%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 45 every morning of 6: 40 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.11%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 26 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 3.8wt.%, and the lipid activity substance content is up to 37.3wt.%.Through further processing, can be used as the immune response medicinal application in clinical.
Embodiment four:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of Japanese apricot; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 7 o'clock every mornings, at 13 o'clock at noon, respectively sprayed the Oolong tea leaching liquor once at 7 o'clock at dusk, the amount of spraying is every square metre and sprays 40g at every turn, and the concentration of Oolong tea leaching liquor is Oolong tea and water ratio 1:38 weight ratio; The extraction temperature of lixiviate Oolong tea is 92 ℃, and extraction time is 15 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 6.3cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 12 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 28 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 92%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, 6: 45 extremely at dusk 8: 50 every morning, the 180 minutes employing censers in every interval were lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.12%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 27.3 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 3.2wt.%, and the lipid activity substance content is up to 37.2wt.%.Through further processing, can be used as acquired immune deficiency syndrome (AIDS) or cancer adjuvant therapy medicament or immune response medicinal application in clinical.
Embodiment five:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed leaching liquor that black tea and green tea mixes at 8 o'clock at dusk once, the amount of spraying is every square metre and sprays 35g at every turn, and the concentration of black tea and green tea leaching liquor is tea and water ratio 1:36 weight ratio; Black tea and green tea ratio 1:1 weight ratio; The black tea of lixiviate and green tea extraction temperature are 98 ℃, and extraction time is 18 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 7.8cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 12 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 27 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 86%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 50 every morning of 6: 55 timesharing extremely at dusk, the 200 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.11%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 26 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 3.9wt.%, and the lipid activity substance content is up to 37.2wt.%.Through further processing, can be used as the immune response medicinal application in clinical.
Embodiment six:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of rice sweet oak tree; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the green tea leaching liquor once at 8 o'clock at dusk, the amount of spraying is every square metre and sprays 45g at every turn, and the concentration of green tea leaching liquor is green tea and water ratio 1:34 weight ratio; The extraction temperature of lixiviate green tea is 95 ℃, and extraction time is 8 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 8.5cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 8 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 34 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 87%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 38 every morning of 6: 45 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.10%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 26.8 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 4.6wt.%, and the lipid activity substance content is up to 37.8wt.%.Through further processing, can be used as cancer adjuvant therapy medicament and be applied to clinical.
Embodiment seven:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of Japanese apricot; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed blue or green tea leaching liquor once at 8 o'clock at dusk, the amount of spraying is every square metre and sprays 32g at every turn, and the concentration of blue or green tea leaching liquor is blue or green tea and water ratio 1:30 weight ratio; The extraction temperature of the blue or green tea of lixiviate is 93 ℃, and extraction time is 12 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 6.7cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 13 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 33 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 90%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 55 every morning of 6: 40 timesharing extremely at dusk, the 220 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.10%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 3.9wt.%, and the lipid activity substance content is up to 35wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Embodiment eight:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed leaching liquor that blue or green tea and Oolong tea mix at 8 o'clock at dusk once, the amount of spraying is every square metre and sprays 48g at every turn, and the concentration of blue or green tea and Oolong tea leaching liquor is tea and water ratio 1:35 weight ratio; Blue or green tea and Oolong tea ratio 1:1 weight ratio; The extraction temperature of blue or green tea of lixiviate and Oolong tea is 100 ℃, and extraction time is 9 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 7.3cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 10 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 30 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 90%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 30 every morning of 6: 30 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.11%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 26 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 4.2wt.%, and the lipid activity substance content is up to 38wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Embodiment nine:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of maple; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the black tea leaching liquor once at 8 o'clock at dusk, the amount of spraying is every square metre and sprays 22g at every turn, and the concentration of black tea leaching liquor is black tea and water ratio 1:40 weight ratio; The extraction temperature of lixiviate black tea is 93 ℃, and extraction time is 17 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 7.8cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 6 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 26 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 90%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 30 every morning of 6: 30 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.15%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 27 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 4.53wt.%, and the lipid activity substance content is up to 40.9wt.%.Through further processing, can be used as cancer adjuvant therapy medicament and be applied to clinical.
Embodiment ten:
1, adopts conventional artificial greenhouse to cultivate on the basis of the method for purple sesame and program and carry out following technique improvement.
2, the place is selected the higher highland that ventilates away from the pollution-free source of the signs of human habitation of height above sea level to build purple sesame on the sunny side and is cultivated booth; Cultivate purple sesame section wood and select qualified after testing hard section wood of robur; Get free of contamination mountain spring water and cultivate relative moisture and the shower water of purple sesame nurturing period in the booth as the purple sesame of regulation and control.
3, differentiate cap until the growth and development stage that produces basidiospore in purple sesame growth, 8 o'clock every mornings, at 12 o'clock at noon, respectively sprayed the Oolong tea leaching liquor once at 8 o'clock at dusk, the amount of spraying is every square metre and sprays 30g at every turn, and the concentration of Oolong tea leaching liquor is Oolong tea and water ratio 1:43 weight ratio; The extraction temperature of lixiviate Oolong tea is 98 ℃, and extraction time is 20 minutes.
4, differentiate cap in purple sesame growth, when purple sesame bacteria cover diameter reaches 5cm, 8 o'clock at dusk every day the purple sesame cultivation booth ventilating opening and the door curtain that keeps out the wind are opened wide, it is 5 ℃ that regulation and control purple at night sesame is cultivated the interior room temperature of booth; 8 o'clock every mornings, purple sesame was cultivated the booth ventilating opening and the door curtain lowering of keeping out the wind is closed, and it is 28 ℃ that regulation and control purple sesame on daytime is cultivated the interior room temperature of booth; Regulating the interior relative moisture of purple sesame cultivation on daytime booth is 92%.
5, before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, in 8: 30 every morning of 6: 30 timesharing extremely at dusk, the 180 minutes employing censers in every interval are lighted the straw fumigation and are produced CO
2, regulate and control in the purple sesame cultivation booth to fill the air CO
2Concentration reaches 0.12%.
6, the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25 ℃.The new light violet Ganoderma lucidum spore that will spray the same day was in time gathered at dusk and drying on the same day.Testing result shows that the purple sesame of results and purple Ganoderma lucidum spore quality are superior, and purple sesame cap stem quality is abundant.The purple Ganoderma lucidum spore of results adopts the chemical analysis method of standard to detect, and lipid active ingredient content such as the interior triterpene compound of purple Ganoderma lucidum spore are up to 3.95wt.%, and the lipid activity substance content is up to 38wt.%.Through further processing, can be used as the acquired immune deficiency syndrome (AIDS) adjuvant therapy medicaments and be applied to clinical.
Claims (5)
1. a method that promotes and promote purple sesame and purple Ganoderma lucidum spore quality is cultivated purple sesame with hard section wood of broad-leaved tree, grows purple sesame to differentiate cap until the growth and development stage that produces basidiospore, sprays the tealeaves leaching liquor; Reach more than the 5cm growth and development stage before produce basidiospore at purple sesame bacteria cover diameter, regulate and control purple sesame and cultivate day and night temperature in the booth, make the night temperature be lower than 13～32 ℃ of daytimes; Before the purple sesame generation basidiospore stage, extremely purple Ganoderma lucidum spore was gathered, adopt CO
2Stimulate, regulate and control purple sesame and cultivate CO in the booth
2Concentration reaches 0.10-0.15%; The purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore is cultivated in the booth round the clock, and room temperature is 25-28 ℃.
2. in accordance with the method for claim 1, it is characterized in that described tealeaves leaching liquor is obtained by following method: with tealeaves and water in tealeaves: the ratio of water=1:30-40 weight ratio at 90-100 ℃ of following lixiviate 5-20 minute, obtains required tealeaves leaching liquor; Used local tea variety is one or more in black tea, green tea, blue or green tea, the Oolong tea.
3. according to claim 1 or 2 described methods, it is characterized in that the concrete steps of this method are as follows:
(1), select for use hard section wood of broad-leaved tree to cultivate purple sesame;
(2), grow purple sesame and to differentiate cap until the growth and development stage that produces basidiospore, spray the tealeaves leaching liquor respectively once in morning every day, noon, evening, the amount of spraying is every square metre and sprays 20-50g at every turn;
(3), reach the growth and development stage of 5～8.5cm before produce basidiospore, regulation and control purple sesame at night cultivates that room temperature is 3-14 ℃ in the booth, and regulation and control purple sesame on daytime cultivates that room temperature is 27-35 ℃ in the booth at purple sesame bacteria cover diameter; Simultaneously, regulating the interior relative moisture of purple sesame cultivation on daytime booth is 85-95%;
(4), before purple sesame produces basidiospore stage to purple Ganoderma lucidum spore and gathers, begin at dusk in every morning, every interval was adopted common censer to light the straw fumigation in 180～240 minutes and is produced CO
2Method, regulate and control purple sesame and cultivate in the booth and fill the air CO
2Concentration reaches 0.10-0.15%;
(5), the purple sesame of regulation and control of gathering during the purple Ganoderma lucidum spore cultivates in the booth round the clock that room temperature is 25-28 ℃.
4. according to claim 1 or 2 described methods, it is characterized in that hard section wood of described broad-leaved tree comprises the wooden hard Duan Mu of maple, rice sweet oak, evergreen chinquapin, robur or Japanese apricot.
5. in accordance with the method for claim 3, it is characterized in that hard section wood of described broad-leaved tree comprises the wooden hard Duan Mu of maple, rice sweet oak, evergreen chinquapin, robur or Japanese apricot.
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| 栎叶、废弃茶叶栽培灵芝试验. 闫茂华.食用菌，第02期. 2000 |
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