CN110257261A - A kind of production method of selenium-rich antrodia mycelia - Google Patents
A kind of production method of selenium-rich antrodia mycelia Download PDFInfo
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- CN110257261A CN110257261A CN201910599061.7A CN201910599061A CN110257261A CN 110257261 A CN110257261 A CN 110257261A CN 201910599061 A CN201910599061 A CN 201910599061A CN 110257261 A CN110257261 A CN 110257261A
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- antrodia camphorata
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- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 47
- 239000011669 selenium Substances 0.000 title claims abstract description 47
- 241000123370 Antrodia Species 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 66
- 230000004151 fermentation Effects 0.000 claims abstract description 66
- 241001486992 Taiwanofungus camphoratus Species 0.000 claims abstract description 56
- 229940091258 selenium supplement Drugs 0.000 claims abstract description 46
- 238000000034 method Methods 0.000 claims abstract description 31
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 26
- 150000003342 selenium Chemical class 0.000 claims abstract description 26
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 20
- 239000008103 glucose Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 17
- 240000008042 Zea mays Species 0.000 claims abstract description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 15
- 235000005822 corn Nutrition 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 244000068988 Glycine max Species 0.000 claims abstract description 14
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 14
- 235000013312 flour Nutrition 0.000 claims abstract description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 14
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 14
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229930003451 Vitamin B1 Natural products 0.000 claims abstract description 13
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 13
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 13
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 13
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 13
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 13
- 239000011781 sodium selenite Substances 0.000 claims abstract description 13
- 229960003495 thiamine Drugs 0.000 claims abstract description 13
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims abstract description 13
- 239000011691 vitamin B1 Substances 0.000 claims abstract description 13
- 235000010374 vitamin B1 Nutrition 0.000 claims abstract description 13
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 3
- 235000001727 glucose Nutrition 0.000 claims abstract 2
- 239000002609 medium Substances 0.000 claims description 46
- 239000007788 liquid Substances 0.000 claims description 28
- 239000001963 growth medium Substances 0.000 claims description 21
- 238000001035 drying Methods 0.000 claims description 17
- 238000011218 seed culture Methods 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 12
- 238000003825 pressing Methods 0.000 claims description 10
- 238000010899 nucleation Methods 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 229960003390 magnesium sulfate Drugs 0.000 abstract 1
- 239000002904 solvent Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 11
- 230000004913 activation Effects 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 238000009835 boiling Methods 0.000 description 9
- 244000061456 Solanum tuberosum Species 0.000 description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 235000012015 potatoes Nutrition 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000011534 incubation Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 230000006835 compression Effects 0.000 description 4
- 238000007906 compression Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000012265 solid product Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- -1 triterpene compound Chemical class 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 description 2
- 108700016172 Glutathione peroxidases Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 101710186708 Agglutinin Proteins 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 241000723347 Cinnamomum Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- KTHDTJVBEPMMGL-VKHMYHEASA-N N-acetyl-L-alanine Chemical compound OC(=O)[C@H](C)NC(C)=O KTHDTJVBEPMMGL-VKHMYHEASA-N 0.000 description 1
- RVSTWRHIGKXTLG-UHFFFAOYSA-N Pangamic acid Natural products CC(C)N(C(C)C)C(N(C(C)C)C(C)C)C(=O)OCC(O)C(O)C(O)C(O)C(O)=O RVSTWRHIGKXTLG-UHFFFAOYSA-N 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241000222383 Polyporales Species 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229940055705 pangamic acid Drugs 0.000 description 1
- ZQTHOIGMSJMBLM-BUJSFMDZSA-N pangamic acid Chemical compound CN(C)CC(=O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O ZQTHOIGMSJMBLM-BUJSFMDZSA-N 0.000 description 1
- 108700024047 pangamic acid Proteins 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229950001390 sudismase Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of production methods of selenium-rich antrodia mycelia.The method includes carrying out Antrodia camphorata strain fermented and cultured in the Antrodia camphorata fermentation medium containing selenium salt.Antrodia camphorata fermentation medium containing selenium salt of the invention is made of corn flour, soybean powder, glucose, potassium dihydrogen phosphate, sodium selenite, magnesium sulfate, vitamin B1 and water.Be experimentally confirmed: the large scale production method of selenium-rich antrodia mycelia of the invention can obtain the selenium-rich antrodia mycelia that organic selenium content significantly improves, and the double effects with selenium and Antrodia camphorata are with good economic efficiency.
Description
Technical field
The invention belongs to eat the bacterium technical field of producing of medicine two, and in particular to a kind of life of selenium-rich antrodia mycelia
Production method.
Background technique
Antrodia camphorata also known as cinnamomum kanahirai hay mushroom, belong to Aphyllophorales, Polyporaceae, antrodia karst, perennial gill fungus mushroom, and Latin is entitled
Antrodia camphorata is the novel species just delivered by biochemical boundary nineteen ninety.Antrodia camphorata is grown in TaiWan, China mountain area height above sea level
It is the peculiar mushroom class in Taiwan between 450-2000 meters of mountain forests.
Antrodia camphorata has many physiologically active ingredients, such as triterpene compound, polysaccharide body, adenosine, Sudismase, protein
(containing immune protein), microelement, vitamin, agglutinin, nucleic acid, cellulose, amino acid etc..Its contained triterpene compound has
More than 200 kinds or more.Antrodia camphorata has extensive pharmacological action, such as anti-cancer, antitumor and hepatoprotective effect.Antrodia camphorata is in addition to being used to
While preventing, treating tumour and cancer cell diffusion, transfer, moreover it is possible to eliminate cancer ascites, the play common to cancer latter stage sufferer
Bitterly, loss of appetite and radiotherapy, side effects of chemotherapy etc. have quite significant improvement.
Selenium is micro elements needed by human, is the indispensable component part of glutathione peroxidase, glutathione
Peroxidase has the function of very strong body biomembrane being protected not influenced and free radical damage by lipid peroxide.Largely
Zoopery confirms that selenium has good anticancer, anti-aging, and strengthen immunity enhances reproductive function, adjusts the suction of vitamin
Receipts such as utilize at the pharmacological actions.
Organic selenium content generally only has 3ppm in existing antrodia mycelia, and content is very low.
Summary of the invention
The object of the present invention is to provide a kind of production methods of selenium-rich antrodia mycelia that organic selenium content significantly improves.
The production method of selenium-rich antrodia mycelia provided by the invention includes by Antrodia camphorata bacterial strain in the ox containing selenium salt
The step of fermented and cultured is carried out in antrodia fermentation medium.
Further, the selenium salt can be sodium selenite.
Further, mass fraction of the sodium selenite in the Antrodia camphorata fermentation medium can be (0.05-
0.2) %.In a specific embodiment of the present invention, mass fraction of the sodium selenite in the Antrodia camphorata fermentation medium
It is 0.05%, 0.12% or 0.2%.
In the above method, the Antrodia camphorata fermentation medium includes corn flour, soybean powder, glucose, potassium dihydrogen phosphate, sulphur
Sour magnesium and vitamin B1.
Further, the Antrodia camphorata fermentation medium containing selenium salt is by the corn flour, the soybean powder, the Portugal
Grape sugar, the potassium dihydrogen phosphate, the sodium selenite, the magnesium sulfate, the vitamin B1 and water composition；
Further, mass fraction of the corn flour in the Antrodia camphorata fermentation medium can be (2-5) %.?
In specific embodiments of the present invention, mass fraction of the corn flour in the Antrodia camphorata fermentation medium is 2%, 3.5%
Or 5%.
Mass fraction of the soybean powder in the Antrodia camphorata fermentation medium can be (1-2) %.In tool of the invention
In body embodiment, mass fraction of the glutinous millet powder in the Antrodia camphorata fermentation medium is 1%, 1.5% or 2%.
Mass fraction of the glucose in the Antrodia camphorata fermentation medium can be (1-3) %.In tool of the invention
In body embodiment, mass fraction of the glucose sugar in the Antrodia camphorata fermentation medium is 1%, 2% or 3%.
Mass fraction of the potassium dihydrogen phosphate in the Antrodia camphorata fermentation medium can be (0.1-0.5) %.At this
In the specific embodiment of invention, mass fraction of the potassium dihydrogen phosphate in the Antrodia camphorata fermentation medium be 0.1%,
0.3% or 0.5%.
Mass fraction of the magnesium sulfate in the Antrodia camphorata fermentation medium can be (0.05-0.2) %.In the present invention
Specific embodiment in, mass fraction of the magnesium sulfate in the Antrodia camphorata fermentation medium be 0.05%, 0.1% or
0.2%.
Concentration of the vitamin B1 in the Antrodia camphorata fermentation medium can be (50-100) ppm.Of the invention
In specific embodiment, mass fraction of the vitamin B1 in the Antrodia camphorata fermentation medium be 50ppm, 80ppm or
100ppm。
In the above method, the pH value of the Antrodia camphorata fermentation medium containing selenium salt can be 3.8-4.0.Specifically, institute
The pH value for stating the Antrodia camphorata fermentation medium containing selenium salt is 3.8,3.9 or 4.0.
The above method includes the following steps:
(1) Antrodia camphorata strain is seeded in seed culture medium and is cultivated, obtain seed liquor；
(2) the seed liquor negative pressure is sucked in 300 liters of fermentors containing fermentation medium and carries out level-one amplification culture,
Obtain one grade fermemtation liquid；
(3) the one grade fermemtation liquid negative pressure is sucked and carries out second level amplification in 1.5 tons of fermentors containing fermentation medium
Culture, obtains second order fermentation liquid；
(4) the second order fermentation liquid negative pressure is sucked in 6 tons of fermentors and the Antrodia camphorata containing selenium salt is added and fermented
Culture medium carries out fermented and cultured, obtains tunning.
In the above method, further include the steps that activating Antrodia camphorata strain before (1).
The method of the activation can specifically carry out in accordance with the following steps: Antrodia camphorata strain is inoculated in PDA solid medium
On, 25 DEG C constant temperature incubation 15-20 days, the strain after being activated.
The PDA solid medium specific adds boiling to boil 20-30 the preparation method is as follows: weighing 200 grams of potatoes is cut into block
Minute, filtered through gauze, heating adds 20 grams and 10 grams of agar of glucose, adds water to 1000 milliliters, 121 DEG C sterilize 30 minutes.
In the above method, (1) includes the following steps:
(1-1) cultivates the strain after activation in 1 liter of triangular flask containing 300 milliliters of seed culture mediums 1, obtains
Primary seed solution；
(1-2) cultivates the primary seed solution in 30 liters of seeding tanks containing 15 liters of seed culture mediums 2, obtains
Secondary seed solution；
Further, in described (1-1), the method for the inoculation is specific as follows: aseptically using the punching of 0.5cm
Bastinade hole, every bottle connects 8 ferfas cake blocks；
The seed culture medium 1 specific adds 20-30 points of boiling boiling the preparation method is as follows: 200 grams of potatoes is taken to be cut into block
Clock, filtered through gauze, heating, then plus 20 grams of glucose, add water to 1000 milliliters, be sub-packed in triangular flask (1000 milliliters), per bottled
300 milliliters of amount, (121 DEG C) of high pressure sterilization 0.1MPa sterilize 30 minutes.
The seed culture medium 2 specific adds 20-30 points of boiling boiling the preparation method is as follows: 5 kilograms of potatoes is taken to be cut into block
Clock, filtered through gauze, heating, then plus 500 grams of glucose, add water to 15 liters, (121 DEG C) of high pressure sterilization 0.1MPa sterilize 30 minutes.
Further, in described (1-1), the condition of the culture is 28 DEG C, 200r/min is cultivated 15-20 days；Specifically
, the condition of the culture is 28 DEG C, 200r/min is cultivated 15 days or 18 days or 20 days.
In (1-2), the condition of the culture is (25-26) DEG C stirring 3-4 days, mixing speed is 100-120 turns/
min.Specifically, the condition of the culture is (25-26) DEG C stirring 3 days or 4 days, mixing speed be 100 turns/min, 110 turns/
Min or 120 turn/min.
In the above method, in (2), the seed liquor negative pressure is sucked to 300 liters of hairs containing 150 liters of fermentation mediums
Level-one amplification culture is carried out in fermentation tank, obtains one grade fermemtation liquid；
The condition of the level-one amplification culture is (25-26) DEG C culture 2-3 days.Specifically, the level-one amplification culture
Condition is (25-26) DEG C culture 2 days or 3 days.
In (3), the one grade fermemtation liquid negative pressure is sucked in 1.5 tons of fermentors containing 750 liters of fermentation mediums
Second level amplification culture is carried out, second order fermentation liquid is obtained；
The condition of the second level amplification culture is (25-26) DEG C culture 2-3 days.Specifically, the second level amplification culture
Condition is (25-26) DEG C culture 2 days or 3 days.
In (2) and (3), the fermentation medium is made of solute and solvent, and solvent is water, solute and its dense
Degree is as follows respectively: corn flour 2-5% (mass fraction), soybean powder 1-2% (mass fraction), glucose 1-3% (mass fraction),
Potassium dihydrogen phosphate 0.1-0.5% (mass fraction), magnesium sulfate 0.05-0.2% (mass fraction), vitamin B1 50-100ppm.
In (4), the second order fermentation liquid negative pressure is sucked in 6 tons of fermentors and contains selenium salt described in 2000 liters of addition
Antrodia camphorata fermentation medium carry out fermented and cultured；
The condition of the fermented and cultured is (25-26) DEG C culture 2-3 days.Specifically, the condition of the fermented and cultured is
(25-26) DEG C culture 2 days or 3 days.
In the above method, the tunning also successively includes the steps that filters pressing, drying and crushes.
The condition of the filters pressing can be the pressure of (50-60) Kg.Specifically, the condition of the filters pressing be 50Kg or 55Kg or
The pressure of 60Kg.
The temperature of the drying can be (60-65) DEG C.Specifically, the temperature of the drying is 60 DEG C or 63 DEG C or 65 DEG C.
The particle diameter that is crushed to can be 60-80 mesh.Specifically, it is described be crushed to particle diameter be 60 mesh or 70 mesh or
80 mesh.
The selenium-rich antrodia mycelia being prepared according to the method described above also belongs to protection scope of the present invention.
The above method also belongs to protection scope of the present invention in the application improved in antrodia mycelia organic selenium content.
The present invention improves the organic selenium content of antrodia mycelia by the conversion technology of selenium, establishes a kind of richness
The production method of selenium antrodia mycelia.It is experimentally confirmed: the large-scale production side of selenium-rich antrodia mycelia of the invention
Method can obtain the selenium-rich antrodia mycelia that organic selenium content significantly improves, the double effects with selenium and Antrodia camphorata, tool
There is good economic benefit.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Corn flour and soybean powder in following embodiments are the products of Guangdong Ying Te health food Co., Ltd；Sodium selenite
It is the product of Hubei Xin Runde Chemical Co., Ltd., product number: 114361163.
The production method of selenium-rich Antrodia camphorata of the invention includes the following steps:
1, actication of culture: Antrodia camphorata strain is inoculated on PDA solid medium, 25 DEG C constant temperature incubation 15-20 days, obtain
Strain after activation.
The PDA solid medium the preparation method is as follows: weighing 200 grams of potatoes is cut into block, add 20-30 points of boiling boiling
Clock, filtered through gauze, heating, then plus 20 grams of glucose and 10 grams of agar, add water to 1000 milliliters, dispense in triangular flask, per bottled
300 milliliters of amount, 121 DEG C sterilize 30 minutes.
2, cultivate strain: the strain after the activation that step 1 is obtained is in 1 liter of triangle containing 300 milliliters of seed culture mediums 1
It is cultivated and (is aseptically punched with the perforation rod of 0.5cm, every bottle connects 8 ferfas cake blocks) in bottle, in isothermal vibration culture
Culture 15-20 days, obtain primary seed solution in case (28 DEG C, 200r/min)；Primary seed solution is seeded to containing 15 liters of seeds
Cultivated in 30 liters of seeding tanks of culture medium 2,25-26 DEG C stirring 3-4 days, mixing speed be 100-120 turn/min, obtain two
Grade seed liquor.
The seed culture medium 1 the preparation method is as follows: 200 grams of potatoes is taken to be cut into block, add boiling to boil 20-30 minutes, yarn
Cloth filtering, heating, then plus 20 grams of glucose, add water to 1000 milliliters, be sub-packed in triangular flask (1000 milliliters), per bottled amount 300
Milliliter, (121 DEG C) of high pressure sterilization 0.1MPa sterilize 30 minutes.
The seed culture medium 2 the preparation method is as follows: 5 kilograms of potatoes is taken to be cut into block, add boiling to boil 20-30 minutes, yarn
Cloth filtering, heating, then plus 500 grams of glucose, add water to 15 liters, (121 DEG C) of high pressure sterilization 0.1MPa sterilize 30 minutes.
3, level-one is amplified: the secondary seed solution negative pressure that step 2 is obtained sucks 300 liters containing 150 liters of fermentation mediums
In fermentor, 25-26 DEG C culture 2-3 days, obtain one grade fermemtation liquid.
The fermentation medium is made of solute and solvent, and solvent is water, and solute and its concentration difference are as follows: corn flour 2-
5% (mass fraction), soybean powder 1-2% (mass fraction), glucose 1-3% (mass fraction), potassium dihydrogen phosphate 0.1-0.5%
(mass fraction), magnesium sulfate 0.05-0.2% (mass fraction), vitamin B1 50-100ppm.
4, second level is amplified: the one grade fermemtation liquid negative pressure that step 3 is obtained sucks 1.5 tons containing 750 liters of fermentation mediums
In fermentor, 25-26 DEG C culture 2-3 days, obtain second order fermentation liquid.
5, add selenium salt culture medium: the second order fermentation liquid negative pressure of step 4 is sucked in 6 tons of fermentors, and 2000 liters of selenium are added
Salt culture medium, 25-26 DEG C fermented and cultured 2-3 days, the pH value of fluid nutrient medium is 3.8-4.0 when fermentation, obtains tunning.
The selenium salt culture medium is made of solute and solvent, and solvent is water, and solute and its concentration difference are as follows: corn flour 2-
5% (mass fraction), soybean powder 1-2% (mass fraction), glucose 1-3% (mass fraction), potassium dihydrogen phosphate 0.1-0.5%
(mass fraction), sodium selenite 0.05-0.2% (mass fraction), magnesium sulfate 0.05-0.2% (mass fraction), vitamin B1
50-100ppm。
6, plate compression: the tunning that step 5 is obtained takes out, and with the pressure filters pressing of 50-60Kg, press dry, consolidate
Body product.
7, drying and crushing: the solid product that step 6 is obtained is placed in drying in vacuum, and drying temperature is 60-65 DEG C, dry
It crushes after the completion, obtains the selenium-rich antrodia mycelia product that particle diameter is 60-80 mesh, which is 75-
150ppm。
The production method of embodiment 1, selenium-rich Antrodia camphorata
1, actication of culture: Antrodia camphorata Taiwanofungus camphoratus (is protected purchased from China General Microbiological strain
Hide administrative center, bacterium numbering be CGMCC 5.906) be inoculated on PDA solid medium, 25 DEG C constant temperature incubation 15 days, obtain
Strain after activation.
2, cultivate strain: the strain after the activation that step 1 is obtained is in 1 liter of triangle containing 300 milliliters of seed culture mediums 1
(aseptically punched with the perforation rod of 0.5cm, every bottle connects 8 ferfas cake blocks) in bottle, isothermal vibration incubator (28 DEG C,
Culture 15 days, obtain primary seed solution in 200r/min)；Primary seed solution is seeded to 30 containing 15 liters of seed culture mediums 2
It rises in seeding tank and is cultivated, 25-26 DEG C is stirred 3 days, and mixing speed is 100 turns/min, obtains secondary seed solution.
3, level-one is amplified: the seed liquor negative pressure that step 2 is obtained sucks 300 liters of seeds containing 150 liters of fermentation mediums
In tank, 25-26 DEG C is cultivated 2 days, obtains one grade fermemtation liquid.
4, second level is amplified: the one grade fermemtation liquid negative pressure that step 3 is obtained sucks 1.5 tons containing 750 liters of fermentation mediums
In seeding tank, 25-26 DEG C is cultivated 2 days, obtains second order fermentation liquid.
5, add selenium salt culture medium: the second order fermentation liquid negative pressure that step 4 is obtained sucks in 6 tons of fermentors, and is added 2000
Rise selenium salt culture medium, 25-26 DEG C fermented and cultured 2 days, the pH value of fluid nutrient medium is 3.8 when fermentation, obtains tunning.
The selenium salt culture medium is made of solute and solvent, and solvent is water, and solute and its concentration difference are as follows: corn flour
2% (mass fraction), soybean powder 1% (mass fraction), glucose 1% (mass fraction), (quality point of potassium dihydrogen phosphate 0.1%
Number), sodium selenite 0.05% (mass fraction), magnesium sulfate 0.05% (mass fraction), pangamic acid 0ppm.
6, plate compression: the tunning that step 5 is obtained takes out, and with the pressure filters pressing of 50Kg, press dry, and obtains solid production
Object.
7, drying and crushing: the solid product that step 6 is obtained is placed in drying in vacuum, and drying temperature is 60 DEG C, has dried
At rear crushing, the selenium-rich antrodia mycelia product of 60 mesh of particle diameter is obtained.
8, it is produced using colorimetric spectrophotometer method (National Standard Method (GB 5009.93-2017)) detection selenium-rich antrodia mycelia
Organic selenium content in object.
The result shows that: the organic selenium content in selenium-rich antrodia mycelia product is 75ppm.
The production method of embodiment 2, selenium-rich Antrodia camphorata
1, actication of culture: Antrodia camphorata Taiwanofungus camphoratus (is protected purchased from China General Microbiological strain
Hide administrative center, bacterium numbering be CGMCC 5.906) be inoculated on PDA solid medium, 25 DEG C constant temperature incubation 20 days, obtain
Strain after activation.
2, cultivate strain: the strain after the activation that step 1 is obtained is in 1 liter of triangle containing 300 milliliters of seed culture mediums 1
(aseptically punched with the perforation rod of 0.5cm, every bottle connects 8 ferfas cake blocks) in bottle, isothermal vibration incubator (28 DEG C,
Culture 20 days, obtain primary seed solution in 200r/min)；Primary seed solution is seeded to 30 containing 15 liters of seed culture mediums 2
It rises in seeding tank and is cultivated, 25-26 DEG C is stirred 4 days, and 120 turns/min of mixing speed obtains secondary seed solution.
3, level-one is amplified: the seed liquor negative pressure that step 2 is obtained sucks 300 liters of seeds containing 150 liters of fermentation mediums
In tank, 25-26 DEG C is cultivated 3 days, obtains one grade fermemtation liquid.
4, second level is amplified: the one grade fermemtation liquid negative pressure that step 3 is obtained sucks 1.5 tons containing 750 liters of fermentation mediums
In seeding tank, 25-26 DEG C is cultivated 3 days, obtains second order fermentation liquid.
5, add selenium salt culture medium: the second order fermentation liquid negative pressure that step 4 is obtained sucks in 6 tons of fermentors, and is added 2000
Rise selenium salt culture medium, 25-26 DEG C fermented and cultured 3 days, the pH value of fluid nutrient medium is 4.0 when fermentation, obtains tunning.
The selenium salt culture medium is made of solute and solvent, and solvent is water, and solute and its concentration difference are as follows: corn flour
5% (mass fraction) (is purchased from Guangdong Ying Te health food Co., Ltd), and soybean powder 2% (mass fraction) (is won special purchased from Guangdong
Health food Co., Ltd), glucose 3% (mass fraction), potassium dihydrogen phosphate 0.5% (mass fraction), sodium selenite 0.2%
(mass fraction), magnesium sulfate 0.2% (mass fraction), vitamin B1 100ppm.
6, plate compression: the tunning that step 5 is obtained takes out, and with the pressure filters pressing of 60Kg, press dry, and obtains solid production
Object.
7, drying and crushing: the solid product that step 6 is obtained is placed in drying in vacuum, and drying temperature is 65 DEG C, has dried
At rear crushing, the selenium-rich antrodia mycelia product of 80 mesh of particle diameter is obtained.
8, it is produced using colorimetric spectrophotometer method (National Standard Method (GB 5009.93-2017)) detection selenium-rich antrodia mycelia
Organic selenium content in object.
The result shows that: the organic selenium content in selenium-rich antrodia mycelia product is 150ppm.
The production method of embodiment 3, selenium-rich Antrodia camphorata
1, actication of culture: Antrodia camphorata Taiwanofungus camphoratus (is protected purchased from China General Microbiological strain
Hide administrative center, bacterium numbering be CGMCC 5.906) be inoculated on PDA solid medium, 25 DEG C constant temperature incubation 18 days, obtain
Strain after activation.
2, cultivate strain: the strain after the activation that step 1 is obtained is in 1 liter of triangle containing 300 milliliters of seed culture mediums 1
(aseptically punched with the perforation rod of 0.5cm, every bottle connects 8 ferfas cake blocks) in bottle, isothermal vibration incubator (28 DEG C,
Culture 18 days, obtain primary seed solution in 200r/min)；Primary seed solution is seeded to 30 containing 15 liters of seed culture mediums 2
It rises in seeding tank and is cultivated, 25-26 DEG C is stirred 4 days, and 110 turns/min of mixing speed obtains secondary seed solution.
3, level-one is amplified: the seed liquor negative pressure that step 2 is obtained sucks 300 liters of seeds containing 150 liters of fermentation mediums
In tank, 25-26 DEG C is cultivated 3 days, obtains one grade fermemtation liquid.
4, second level is amplified: the one grade fermemtation liquid negative pressure that step 3 is obtained sucks 1.5 tons containing 750 liters of fermentation mediums
In seeding tank, 25-26 DEG C is cultivated 3 days, obtains second order fermentation liquid.
5, add selenium salt culture medium: the second order fermentation liquid negative pressure that step 4 is obtained sucks in 6 tons of fermentors, and is added 2000
Rise selenium salt culture medium, 25-26 DEG C fermented and cultured 3 days, the pH value of fluid nutrient medium is 3.9 when fermentation, obtains tunning.
The selenium salt culture medium is made of solute and solvent, and solvent is water, and solute and its concentration difference are as follows: corn flour
3.5% (mass fraction) (is purchased from Guangdong Ying Te health food Co., Ltd), and soybean powder 1.5% (mass fraction) (is purchased from Guangdong
Ying Te health food Co., Ltd), glucose 2% (mass fraction), potassium dihydrogen phosphate 0.3% (mass fraction), sodium selenite
0.12% (mass fraction), magnesium sulfate 0.1% (mass fraction), vitamin B1 80ppm.
6, plate compression: the tunning that step 5 is obtained takes out, and with the pressure filters pressing of 55Kg, press dry, and obtains solid production
Object.
7, drying and crushing: the solid product that step 6 is obtained is placed in drying in vacuum, and drying temperature is 63 DEG C, has dried
At rear crushing, the selenium-rich antrodia mycelia product of 70 mesh of particle diameter is obtained.
8, it is produced using colorimetric spectrophotometer method (National Standard Method (GB 5009.93-2017)) detection selenium-rich antrodia mycelia
Organic selenium content in object.
The result shows that: the organic selenium content in selenium-rich antrodia mycelia product is 98ppm.
The present invention is had been described in detail above.To those skilled in the art, do not depart from spirit of the invention and
Range, and without carrying out under unnecessary experimental conditions, can synchronization parameters, concentration and under the conditions of, it is real in a wider range
Apply the present invention.Although The present invention gives particular embodiments, it is understood that, the present invention can be improved further.
In short, pressing the principle of the present invention, the application is intended to include any change, purposes or improvement of the present invention, including departing from this Shen
Please in the open scope, and the change carried out with routine techniques known in the art.By the range of following attached claims,
It can carry out the application of some essential characteristics.
Claims (10)
1. a kind of production method of selenium-rich antrodia mycelia is trained including Antrodia camphorata bacterial strain ferments in the Antrodia camphorata containing selenium salt
Support the step of fermented and cultured is carried out in base.
2. according to the method described in claim 1, it is characterized by: the selenium salt is sodium selenite.
3. according to the method described in claim 2, it is characterized by: the sodium selenite is in the Antrodia camphorata fermentation medium
Mass fraction be (0.05-0.2) %.
4. method according to claim 1 to 3, it is characterised in that: the Antrodia camphorata fermentation medium includes corn
Powder, soybean powder, glucose, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1.
5. method according to claim 1 to 4, it is characterised in that: the Antrodia camphorata fermentation medium containing selenium salt
By the corn flour, the soybean powder, the glucose, the potassium dihydrogen phosphate, the sodium selenite, the magnesium sulfate, institute
State vitamin B1 and water composition；
Mass fraction of the corn flour in the Antrodia camphorata fermentation medium is (2-5) %；
Mass fraction of the soybean powder in the Antrodia camphorata fermentation medium is (1-2) %；
Mass fraction of the glucose in the Antrodia camphorata fermentation medium is (1-3) %；
Mass fraction of the potassium dihydrogen phosphate in the Antrodia camphorata fermentation medium is (0.1-0.5) %；
Mass fraction of the magnesium sulfate in the Antrodia camphorata fermentation medium is (0.05-0.2) %；
Concentration of the vitamin B1 in the selenium salt culture medium is (50-100) ppm.
6. -5 any method according to claim 1, it is characterised in that: the Antrodia camphorata fermentation medium containing selenium salt
PH value be 3.8-4.0.
7. -6 any method according to claim 1, it is characterised in that: described method includes following steps:
(1) Antrodia camphorata strain is cultivated in seed culture medium, obtains seed liquor；
(2) the seed liquor negative pressure is sucked in 300 liters of fermentors containing fermentation medium and carries out level-one amplification culture, obtained
One grade fermemtation liquid；
(3) the one grade fermemtation liquid negative pressure is sucked in 1.5 tons of fermentors containing fermentation medium and carries out second level amplification culture,
Obtain second order fermentation liquid；
(4) the second order fermentation liquid negative pressure is sucked in 6 tons of seeding tanks and the Antrodia camphorata fermented and cultured containing selenium salt is added
Base carries out fermented and cultured, obtains tunning.
8. according to the method described in claim 7, it is characterized by:
In (2), the condition of the level-one amplification culture is (25-26) DEG C culture 2-3 days；
In (3), the condition of the second level amplification culture is (25-26) DEG C culture 2-3 days；
In (4), the condition of the fermented and cultured is (25-26) DEG C culture 2-3 days.
9. according to the method described in claim 7, it is characterized by: the tunning also successively includes filters pressing, drying and powder
Broken step:
Or, the condition of the filters pressing is the pressure of (50-60) Kg；
Or, the temperature of the drying is (60-65) DEG C；
Or, the particle diameter that is crushed to is 60-80 mesh.
10. the selenium-rich antrodia mycelia being prepared according to any method of claim 1-9；
Or, any method of claim 1-9 is improving the application in antrodia mycelia organic selenium content.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066576A (en) * | 2020-01-20 | 2020-04-28 | 张旺凡 | Production technology method of antrodia camphorata mycelium powder with high selenium content and high triterpene content |
| CN113875989A (en) * | 2021-09-23 | 2022-01-04 | 山东安为先生物科技有限公司 | Functionalized water-soluble nano-selenium, preparation method and application |
| CN114752641A (en) * | 2022-05-16 | 2022-07-15 | 昆明理工大学 | Preparation method of antrodia camphorata selenium polysaccharide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001005938A1 (en) * | 1999-07-15 | 2001-01-25 | Quancai Li | A method for producing mycelia of ganoderma lucidum containing selenium and other trace elements |
| WO2018018476A1 (en) * | 2016-07-27 | 2018-02-01 | 深圳市仁泰生物科技有限公司 | Antrodia camphorata dish culture method |
| CN108977388A (en) * | 2018-08-09 | 2018-12-11 | 黄铭坚 | A kind of production method and its used medium of selenium-rich bafillus natto |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
-
2019
- 2019-07-04 CN CN201910599061.7A patent/CN110257261A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001005938A1 (en) * | 1999-07-15 | 2001-01-25 | Quancai Li | A method for producing mycelia of ganoderma lucidum containing selenium and other trace elements |
| WO2018018476A1 (en) * | 2016-07-27 | 2018-02-01 | 深圳市仁泰生物科技有限公司 | Antrodia camphorata dish culture method |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
| CN108977388A (en) * | 2018-08-09 | 2018-12-11 | 黄铭坚 | A kind of production method and its used medium of selenium-rich bafillus natto |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066576A (en) * | 2020-01-20 | 2020-04-28 | 张旺凡 | Production technology method of antrodia camphorata mycelium powder with high selenium content and high triterpene content |
| CN113875989A (en) * | 2021-09-23 | 2022-01-04 | 山东安为先生物科技有限公司 | Functionalized water-soluble nano-selenium, preparation method and application |
| CN113875989B (en) * | 2021-09-23 | 2023-09-29 | 山东安为先生物科技有限公司 | Functionalized water-soluble nano-selenium, preparation method and application |
| CN114752641A (en) * | 2022-05-16 | 2022-07-15 | 昆明理工大学 | Preparation method of antrodia camphorata selenium polysaccharide |
| CN114752641B (en) * | 2022-05-16 | 2023-08-22 | 昆明理工大学 | Preparation method of Antrodia camphorata selenium polysaccharide |
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