CN105219745B - 一种固定化转氨酶及其在合成西他列汀中间体中的应用 - Google Patents
一种固定化转氨酶及其在合成西他列汀中间体中的应用 Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
Landscapes
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
编号 | 酶 | 酶活收率(%) | 比酶活(U/g) |
1 | 游离酶 | 100 | 3086 |
2 | 固定化载体A制得的固定化酶 | 50 | 1543 |
3 | 固定化载体B制得的固定化酶 | 27 | 833 |
批次 | 反应时间(h) | 转化率 | 残余酶活 |
1 | 19 | >99% | 99% |
2 | 19 | >99% | 99% |
3 | 19 | >99% | 99% |
4 | 19 | >99% | 99% |
5 | 19 | >99% | 98% |
6 | 19 | >99% | 97% |
7 | 19 | >99% | 98% |
8 | 20 | >99% | 97% |
9 | 20 | >99% | 97% |
10 | 20 | >99% | 96% |
11 | 20 | >99% | 95% |
12 | 20 | >99% | 96% |
13 | 20 | >99% | 95% |
14 | 21 | >99% | 95% |
15 | 21 | >99% | 94% |
16 | 21 | >99% | 94% |
17 | 22 | >99% | 93% |
18 | 22 | >99% | 92% |
19 | 24 | >99% | 91% |
20 | 25 | 99% | 89% |
21 | 28 | 97% | 87% |
Claims (14)
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CN201410262154.8A CN105219745B (zh) | 2014-06-12 | 2014-06-12 | 一种固定化转氨酶及其在合成西他列汀中间体中的应用 |
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CN105219745A CN105219745A (zh) | 2016-01-06 |
CN105219745B true CN105219745B (zh) | 2018-12-04 |
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Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2733477T3 (es) * | 2017-07-04 | 2019-11-29 | Fis Fabbrica Italiana Sintetici Spa | Procedimiento eficaz para la preparación de sitagliptina a través de una preparación muy eficaz del intermedio ácido 2,4,5-trifluorofenilacético |
CN109234327A (zh) * | 2017-07-11 | 2019-01-18 | 上海弈柯莱生物医药科技有限公司 | 一种立体选择性的转氨酶在不对称合成手性胺中的应用 |
CN109251925A (zh) * | 2017-07-14 | 2019-01-22 | 上海蓝木化工有限公司 | 一种离子交换树脂固定化转氨酶的制备及其应用 |
CN110129306A (zh) * | 2018-02-08 | 2019-08-16 | 广东东阳光药业有限公司 | 固定化转氨酶及其应用 |
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