CN105210736B - A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting - Google Patents
A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting Download PDFInfo
- Publication number
- CN105210736B CN105210736B CN201510706290.6A CN201510706290A CN105210736B CN 105210736 B CN105210736 B CN 105210736B CN 201510706290 A CN201510706290 A CN 201510706290A CN 105210736 B CN105210736 B CN 105210736B
- Authority
- CN
- China
- Prior art keywords
- rice
- spermine
- blast
- solution
- leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
Abstract
It includes that planting site prepares, seedling management and spermine induction processing step, spermine induction processing step include to induce the method that processing improves rice bacterial blight resistance and blast resisting, this method the invention discloses a kind of spermine：The solution of spermine that a concentration of 2 10mmol/L (preferably 2 5mmol/L) are continuously poured to the rice in Adult plant, pours 800 1200L of solution of spermine daily per acre, and it is 4 7d to pour total time.This method is easy, easy to operate, takes effect quick, can improve resistance of the rice to bacterial leaf-blight and to rice blast in a short time, can effectively avoid rice in bacterial leaf-blight and loss when being broken out with rice blast, increases economic efficiency.
Description
Technical field
The invention belongs to paddy disease-resistant research fields, and in particular to a kind of white by short time spermine processing raising Rice Resistance
The method of leaf blight and blast resisting, plantation and bacterial blight-resisting and anti-rice blast of this method suitable for any regional rice in China
The paddy growth of disease.
Background technology
Rice is one of important crops for the survival of mankind, generally the edge in the area such as the torrid zone, subtropical zone and temperate zone
Cultivate to the waterflooding of sea plain, tidal delta and river basin.
Bacterial leaf-blight is one of Major Diseases of rice, and diseased plant blade tip and edge are come into being yellow green spot, after sent out along vein
Transform into the long streak of pale asphyxia, yellowish-brown, finally become canescence and it is withered.Diseased plant easily lodges, and the unreal rate of spike of rice increases.Germ is being planted
Overwintering propagation on sub and ill straw.Tillering stage disease starts to develop.High temperature and humidity, storm, rice field are excessive by flood and nitrogenous fertilizer
When be conducive to plant disease epidemic.Bacterial blight of rice has generation in each rice region in China, larger to yield effect, not plump paddy and cracks rice
More, the underproduction reaches 20%-30%, the reachable 50%-60% of weight, or even No kernels or seeds are gathered, as in a year of scarcity.
Rice blast also known as rice blast：It being commonly called as burning pest, cracks a pest between the teeth, rice blast is one of big important disease of rice four,
Field differentiates symptom；Hazard rice each section all occurs in rice entire breeding time.Rice shoot；Become yellowish-brown after the onset and withered
Extremely, apparent scab is not formed, and when moist, it is mould can to grow cinerous.In China south and north, rice region has different degrees of generation, stream
Row time general underproduction 10-20%, the serious underproduction reach 40-50%, fall ill in rice seedling seedling stage and tillering stage, blade can be made big
Measure it is withered, when serious full field in burn shape, though some rice strain it is not withered, extraction young leaves be not easy to extend, plant atrophy is not taken out
Fringe extracts short and small fringe out, and the morbidity of booting heading stage, section pest, panicle blast seriously occur, and can cause a large amount of dead ears or half dead ears, damage
It loses very big.Rice blast can all occur in entire growth period duration of rice, according to the difference in aggrieved period and position, can be divided into seedling pest, leaf
Pest, section pest, panicle blast and grain pest etc., wherein with panicle blast to yield effect maximum.Main cause harm blade, stalk, fringe portion.Cause
Period, the position difference of causing harm are divided into seedling pest, leaf pest, section pest, panicle blast, grain pest.Before seedling pest betide three leaves, by seed-borne fungi
It is caused.Sick seedling base portion is greyish black, top browning, crispaturas and dead, generation a large amount of grey black mould layers in disease portion when humidity is larger, i.e. pathogen
Conidiophore and conidium.Leaf pest can occur in entire breeding time.Tiller to the jointing stage causes harm heavier.
Therefore, Study On Rice bacterial blight-resisting and blast resisting mechanism, to provide can improve rice bacterial blight resistance and
Disease-resistant variety is directly cultivated in the various measures of blast resisting ability, this is obviously significant.
Spermine (Spermin) is the polyamines of amino and two imino groups containing there are two, in vivo by putrescine
(butanediamine) and s-adenosylmethionine generate after a variety of enzymatics, molecular formula C10H26N4, molecular weight 202.It and spermidine are all
It is present in bacterium and most animals cell, is the important substance for promoting cell Proliferation.In acid condition, it appear that it is more
Cationic polyamine class feature, and can be combined with virus with DNA in bacterium.We have discovered that also containing spermine and in plant
Detected in various plants include rice, thus carry out extensively in plant the research of spermine by the nutrition of the mankind and
Agricultural provides very useful information.
Invention content
The present inventor is by furtheing investigate rice bacterial blight resistance and blast resisting mechanism, to provide a kind of raising rice
The method of bacterial blight-resisting and blast resisting.
The method of the present invention mainly induces the rice seedling of processing Adult plant by the spermine of low concentration, reaches raising Rice Resistance
The purpose of bacterial leaf-blight and blast resisting, it is easy to implement the method, it is easy to operate, it takes effect quick, rice dialogue can be improved in a short time
The resistance of leaf blight and blast resisting can effectively avoid rice in bacterial leaf-blight and loss when being broken out with rice blast, improve warp
Ji benefit.
In order to realize that above-mentioned goal of the invention, technical scheme of the present invention overview are as follows：
A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting, this method include planting site standard
Standby, seedling management and spermine induction processing step, spermine induction processing step include：Rice in Adult plant is connected
The continuous solution of spermine for pouring a concentration of 2-10mmol/L (preferably 2-5mmol/L), pours solution of spermine 800- daily per acre
1200L, it is 4-7d to pour total time.
It should be noted that before epiphysin induces processing rice, it is also necessary to do following work：
(1) planting site prepares：Using the mechanism of plough, rake, in conjunction with agricultural technologys such as fertilising, irrigation and efficent rotations,
Keep rice field smooth, for Tanaka without accumulated water, dry field degree is consistent, and it is neat to be conducive to paddy growth；Keep rice field soil block fine crushing simultaneously, clay fertilizer phase
Melt, topsoil is soft, no trash.Then base fertilizer is added, 50 kilograms or so of composite fertilizer is added per acre and (contains potassium sulfate and nitre
State nitrogen, general total nutrient finally flatten bed surface not less than 45%).
(2) seedling management：Can rice seedling first be cultivated in certain block Tanaka, after planting reveal point to 1 leaf, temperature is to keep the temperature
Main, temperature is at 25-30 DEG C, and 25-28 DEG C of optimum temperature, the 2 leaf phases are kept for 25 DEG C, and 3 leaf phases were kept for 20-22 DEG C.Nighttime temperature is relatively low,
Should take in greenhouse and open electric light or point burns the measures such as rice husk and heats, hut nursery will outside canopy cover straw or it is cold-proof by etc. arrange
It applies and heats, it is ensured that reach the minimum temperature index needed for each leaf age of rice shoot, make seed rice can normal growth and development on seedbed.In seedling
Seedling grow tall about 8 centimeters when, by pulling up seedlings rice transplanting, rice transplanting, machine transplanting of rice etc. by hand, the fair weather of cold tail is selected to start to transplant work；
Rice transplanting will be accomplished shallow, even, straight, steady, and transplant depth half is no more than 3 centimetres, and accomplishes rice shoot band fertilizer, band soil.Nursery initial stage fits
Work as watering, after 40 days, is irrigated according to natural situation rainwater.
It is further preferred that the method that spermine induction processing improves rice bacterial blight resistance as described above, wherein starting to fill
Pour Rice Seedling Height 40-60cm when solution of spermine.
It is further preferred that the method that spermine induction processing improves rice bacterial blight resistance as described above, wherein continuous fill
Pour solution of spermine time be 5d, 1 time/d.
(i.e. contain 0.282g in every liter of water by the solution of spermine 2 or 5mmol/L of above short time (5 days) low concentration
Or 0.705g spermine) processing, it is found that processed rice material ratio does not have processed rice material in bacterial leaf-blight and rice blast
Growing state is good when disease infects, and it is more shorter to show as scab length on blade, more disease-resistant.As shown in figure 1, exist
After solution of spermine 2 or 5mmol/L (i.e. containing 0.282g or 0.705g spermine in every liter of water) pretreatment of low concentration, bacterial leaf spot
After disease infects rice leaf 15 days, scab length is 66%-74% when spermine useless pre-processes on rice leaf.Such as attached drawing 1B
It is shown, it is pre-processed with the solution of spermine 2 or 5mmol/L of low concentration (i.e. containing 0.282g or 0.705g spermine in every liter of water)
Afterwards, after rice blast infects rice leaf 15 days, scab length is 70%-77% when spermine useless pre-processes on rice leaf.
Based on the studies above achievement, the present invention also provides purposes of the spermine in terms of Rice Resistance characteristic of disease physiology inductive technology, i.e.,：Spermine
Application in improving rice bacterial blight resistance；Alternatively, application of the spermine in improving rice anti-rice blast.
Compared with prior art, the present invention haing the following advantages and effect：The method of the present invention is easy, easy to operate, takes effect
Fast, rice can be improved in a short time to the resistance of bacterial leaf-blight and blast resisting, can effectively avoid rice in bacterial leaf-blight
And loss when being broken out with rice blast, it increases economic efficiency.
Description of the drawings
Fig. 1 is continuous pouring various concentration solution of spermine induction processing 5 days to rice bacterial blight resistance and blast resisting
Influence schematic diagram.
(A) rice material of normal growth is equally divided into three parts, and first part is normally watered 5 days, and second part is poured 2mmol/L essences
Amine aqueous solution (i.e. containing 0.282g spermine in every liter of water) 5 days, third part is poured 5mmol/L solution of spermine and (is i.e. contained in every liter of water
0.705g spermine) 5 days.Then the rice leaf for taking this three parts of materials picks bacterial leaf spot bacterium solution (OD with scissors600=0.5) water is cut
Rice blade, every group of material are inoculated with the consistent plant of 20 plants of growing ways, and every plant of leaf for cutting 3 full extensions is infecting 5 days, 10 days
With statistics each group scab length at 15 days.
(B) rice material of normal growth is divided into three parts, and first part is normally watered 5 days, and second part is poured 2mmol/L essences
Amine aqueous solution (i.e. containing 0.282g spermine in every liter of water) 5 days, third part is poured 5mmol/L solution of spermine and (is i.e. contained in every liter of water
0.705g spermine) 5 days.Then the rice leaf for taking this three parts of materials, with prepared rice blast fungus spore liquid (1 × 105A/mL)
Artificial spray inoculation, every group of material are inoculated with the consistent plant of 20 plants of growing ways, and each group disease is counted when infecting 5 days, 10 days and 15 days
Spot length.
Specific implementation mode
It is specific embodiment of the present invention below, technical scheme of the present invention is described further, but this hair
Bright protection domain is not limited to the embodiment.It is every to be included in this hair without departing substantially from the change of present inventive concept or equivalent substitute
Within bright protection domain.
Embodiment 1：Spermine induction processing improves the experimental study of rice bacterial blight resistance and blast resisting
1, planting site prepares：
Mechanism using plough, rake keeps rice field smooth, Tanaka in conjunction with agricultural technologys such as fertilising, irrigation and efficent rotations
Without accumulated water, dry field degree is consistent, and it is neat to be conducive to paddy growth；Keep rice field soil block fine crushing simultaneously, clay fertilizer mutually melts, and topsoil is soft, nothing
Trash.Then base fertilizer is added, it is (general total foster containing potassium sulfate and nitrate nitrogen that 50 kilograms or so of composite fertilizer is added per acre
Divide not less than 45%), finally flattens bed surface.
2, seedling management：
Rice seedling first is cultivated in certain block Tanaka, after planting reveals point to 1 leaf, temperature is based on keeping the temperature, and temperature is in 25-30
DEG C, 25-28 DEG C of optimum temperature, the 2 leaf phases are kept for 25 DEG C, and 3 leaf phases were kept for 20-22 DEG C.Nighttime temperature is relatively low, should take in greenhouse and open
Electric light or point burn the measures such as rice husk and heat, and hut nursery cover straw or cold-proof will be heated outside canopy by equal measures, it is ensured that reach
To the minimum temperature index needed for each leaf age of rice shoot, make seed rice can normal growth and development on seedbed.It grows tall about 8 centimeters in rice shoot
When, by rice transplanting, rice transplanting, the machine transplanting of rice etc. of pulling up seedlings by hand, the fair weather of cold tail is selected to start to transplant work；Rice transplanting to accomplish it is shallow,
Even, straight, steady, transplant depth half is no more than 3 centimetres, and accomplishes rice shoot band fertilizer, band soil.It is nursery initial stage, appropriate to water, 40 days
Afterwards, it is irrigated according to natural situation rainwater.
3, spermine induction is handled：
Choose the rice seedling (high about 50cm) of Adult plant, continuous 5 days (i.e. every liter of solution of spermine for pouring 2 or 5mmol/L
Contain 0.282g or 0.705g spermine in water), pour solution of spermine 1000L daily per acre, once a day.In other times,
It waters according to normal condition, i.e., water 1500L/d per acre；After 40 days, irrigated according to natural situation rainwater.
4, Analysis of Resistance of the rice to bacterial leaf-blight：
The inoculation test of rice leaf spot bacteria strain X oo-PXO99 is carried out to the rice of Adult plant using leaf-cutting method.First
In PSA solid mediums (peptone 10g/L；Sucrose 10g/L；Glutamic acid 1g/L；pH 6.8-7.0；Add agar powder 20g per 1L)
Upper 28 DEG C of activation pathogen, picking single bacterium colony, with PSA fluid nutrient mediums (peptone 10g/L；Sucrose 10g/L；Glutamic acid 1g/L；
PH 6.8-7.0) 180r/min shake bacterium proliferation.Take bacterium solution to cross on solid medium again after 1 day, 28 DEG C in light culture 2 days,
Then bacterium is washed from culture medium with sterile water, bacterial concentration is transferred to OD600=0.5.Rice leaf is chosen in inoculation, uses
Scissors picks bacterial leaf spot bacterium solution (OD600=0.5) rice leaf is cut, every group of material is inoculated with the consistent plant of 20 plants of growing ways, and every plant is cut
The leaf of 3 full extensions counts each group scab length degree when infecting 5 days, 10 days and 15 days.
5, Analysis of Resistance of the rice to rice blast：
The inoculation that rice rice blast bacteria strain Magnaporthe oryzae are carried out to the rice of Adult plant using leaf-cutting method tries
It tests.First in PDA solid mediums (peptone 10g/L；Glucose 20g/L；pH 6.8-7.0；Add agar powder 20g per 1L) on 26
DEG C expand culture 10 days, then the spore of bacterium is washed from culture medium with sterile water, inoculating spores liquid concentration is adjusted to 1 ×
105A/mL.Rice leaf is chosen in inoculation, by prepared spore liquid (1 × 105A/mL) artificial spray inoculation, every group of material connect
The consistent plant of 20 plants of growing ways of kind, each group scab length is counted when infecting 5 days, 10 days and 15 days.
It is handled by above short time (5 days) the low concentration solution of spermine, the rice material ratio that as a result display processing is crossed does not have
There is processed rice material growing state when bacterial leaf-blight and rice blast infect good, shows as on blade scab length more
It is shorter, it is more disease-resistant.As shown in Figure 1A, (i.e. contain in every liter of water with the solution of spermine 2 or 5mmol/L of low concentration
0.282g or 0.705g spermine) pretreatment after, after bacterial leaf-blight infects rice leaf 15 days, on rice leaf scab length be not
66%-74% when being pre-processed with spermine.As shown in Figure 1B, in solution of spermine 2 or 5mmol/L (i.e. every liter of water with low concentration
In contain 0.282g or 0.705g spermine) pretreatment after, after rice blast infects rice leaf 15 days, scab length on rice leaf
70%-77% when being pre-processed for spermine useless.
Claims (1)
1. a kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting, which is characterized in that this method packet
Including planting site preparation, seedling management and spermine induction processing step, spermine induction processing step includes：To being in strain
The rice of phase continuously pours the solution of spermine of a concentration of 2-5mmol/L, starts to pour Rice Seedling Height 40- when solution of spermine
60cm, pours solution of spermine 800-1200L daily per acre, continuously pour solution of spermine time be 5d, 1 time/d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510706290.6A CN105210736B (en) | 2015-10-27 | 2015-10-27 | A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510706290.6A CN105210736B (en) | 2015-10-27 | 2015-10-27 | A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105210736A CN105210736A (en) | 2016-01-06 |
| CN105210736B true CN105210736B (en) | 2018-10-09 |
Family
ID=54980361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510706290.6A Active CN105210736B (en) | 2015-10-27 | 2015-10-27 | A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105210736B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110024611B (en) * | 2019-05-24 | 2020-12-22 | 海南大学 | Method for improving disease resistance of betel nuts through arecoline treatment and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104770278A (en) * | 2015-04-03 | 2015-07-15 | 广西经正科技开发有限责任公司 | Intensive cultivation method for dendrobium officinale in greenhouse |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6183851B2 (en) * | 2013-08-22 | 2017-08-23 | 国立研究開発法人農業・食品産業技術総合研究機構 | Soil infectious disease control method and testing method for microorganisms for soil infectious disease control |
-
2015
- 2015-10-27 CN CN201510706290.6A patent/CN105210736B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104770278A (en) * | 2015-04-03 | 2015-07-15 | 广西经正科技开发有限责任公司 | Intensive cultivation method for dendrobium officinale in greenhouse |
Non-Patent Citations (4)
| Title |
|---|
| 亚精胺对黄瓜幼苗灰霉病的诱抗作用;尚庆茂、张志刚;《应用生态学》;20080430;第19卷(第4期);第825-830页 * |
| 外源亚精胺对甜瓜幼苗白粉病抗性的影响;李小玲等;《西北植物学报》;20150930;第35卷(第9期);第1800-1807页 * |
| 多胺在提高柑橘溃疡病抗性中的作用及其机理研究;杨枫;《中国优秀硕士学位论文全文数据库-农业科技辑》;20140915(第9期);第D046-85页 * |
| 水杨酸（SA）诱导植物对病虫害产生抗性及作用机制研究;龙亚芹等;《热带农业科学》;20091231;第29卷(第12期);第46-50页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105210736A (en) | 2016-01-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
| CN103430805B (en) | Shaping cultivation method of peach tree shape with two boughs and one center truck | |
| CN104160902B (en) | Do not know the assorted mandarin orange off-season cultivation method of fire | |
| CN105379534A (en) | High-quality and high-yield planting method for kudzuvine roots | |
| CN104255353B (en) | A kind of yam cultivation organic mat long and preparation method and application | |
| CN105145261A (en) | Method for cultivating hybrid oranges | |
| CN105104046B (en) | A kind of method that epiphysin induction processing improves rice bacterial blight resistance | |
| CN105594412A (en) | Cultivation method of edible chrysanthemum flowers | |
| CN103688697A (en) | Cultivation method for puerarin | |
| CN103875498A (en) | Method for planting malus halliana | |
| CN105613024A (en) | Organic cultivation and rotation method for watermelons, rice and water chestnuts in spring and summer | |
| CN103650919A (en) | Full-sun cultivation method of black fungus | |
| CN103880487A (en) | Three-in-one green manure fermentation nutritional agent and application method thereof | |
| CN103329802A (en) | Pollution-free high-yield planting method for strawberries | |
| CN104871915A (en) | Cultivation method of Taiwan psidium guajava in Karst rock-desertification areas | |
| CN104956981A (en) | Cultivating method for big fruit hawthorn trees on self-conservation stony desertification land | |
| CN102845194A (en) | Method for culturing purple sweet potatoes Jingshu-6 | |
| CN105191646A (en) | Bitter gourd planting method | |
| CN105123242A (en) | Ecological wintering method for planting passion fruit in temperate area | |
| CN109496656A (en) | A method of improving Folium Ginkgo quality and harvest efficiency | |
| CN102893786A (en) | Paddy-upland rotation culture method for strawberries and water chestnuts | |
| CN1335052A (en) | Crab grass planting method | |
| CN105210736B (en) | A kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting | |
| Sardana et al. | Productivity of semi-spreading and bunch type varieties of groundnut as influenced by sowing dates. | |
| CN109452116A (en) | A method of using pine needle meal and cigarette muscle powder culture underglass rhizoma polygonati |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |