JP6183851B2 - Soil infectious disease control method and testing method for microorganisms for soil infectious disease control - Google Patents

Description

  The present invention relates to a soil infectious disease control method that accompanies the growth of crops, a test method for useful microorganisms, a test method for soil pH correction substances, and a test method for plant disease resistance inducers.

  Most plant diseases are infectious diseases caused mainly by microorganisms. In particular, since it is difficult to sufficiently sterilize soil-borne pathogenic microorganisms with chemical agents, soil-borne diseases caused by these microorganisms (hereinafter also referred to as soil diseases) are known as difficult-to-control diseases. Yes. Therefore, a sterilization method (soil reduction disinfection) has been devised to artificially reduce the field soil by applying organic matter and irrigation, but the effect is also unstable. Moreover, in addition to the time and labor required for sterilization of field soil by chemical agents and reduction, it cannot be planted during the implementation period. Furthermore, the main chemical agents (fumigation agents) used for soil sterilization have low selectivity for the organisms that act on them, and act on a wide range of organisms in addition to pathogenic bacteria. There are concerns about the impact. On the other hand, disease resistant varieties have a stable effect of avoiding disease, but if a new pathogenic strain that breaks down the resistance emerges, the effect is drastically reduced. Furthermore, when disease resistant varieties are used, the quality such as taste required by producers and consumers is reduced, and when used as rootstock, time, labor, and outsourcing costs are also increased. Therefore, in order to realize stable production of high-quality crops in harmony with the environment, it is required to develop a new soil disease control technique that overcomes these problems.

  So far, many researches and developments related to biological control of soil diseases using useful microorganisms have been carried out as a basis for technological development to compensate for the disadvantages of soil sterilization and disease resistant varieties (see Patent Documents 1 to 8). ). That is, if the host microorganism is inoculated with the useful microorganism before or at the same time as the pathogenic microorganism contacts the host plant, the degree of disease is reduced. This phenomenon is caused by the combined effects of useful microorganisms on nutritional and physical competition with pathogenic microorganisms, antibiotics, parasitism, and induction of disease resistance of host plants. However, commercially available microbial pesticides have not been actively introduced in the agricultural field because of their poor disease control effect or unstable effects compared to chemical pesticides and disease resistant varieties.

  On the other hand, Brassicaceae root-knot disease (non-patented) by soil pH correction using calcium oxide (quick lime), calcium hydroxide (slaked lime), mashed lime, converter slag, sulfur flower, iron (II) sulfate, ammonium sulfate, etc. Document 1), Fusarium disease (Non-patent document 2), homoposis root rot (Non-patent document 3), potato scab (Non-patent document 4), and the like are known to decrease the degree of occurrence of soil diseases. . However, since the effect is exerted only when the degree of occurrence of the disease is low (see Non-Patent Document 3), it cannot be expected for the control in the field that suffers a great damage.

  In addition, a drug (resistance inducer) that induces disease resistance inherent in plants is known. Resistance inducers are classified into pesticides (bactericides), and in Japan, isothianyl, thiazinyl and probenazole, which are mainly used for controlling rice blast, are commercially available. Of these three agents, probenazole is registered as an agrochemical for diseases that occur on the ground such as cruciferous vegetables when applied to the soil, and for lettuce infection from the underground. Although it is weak against root rot, it has been reported to show a controlling effect (Non-patent Document 5). However, soil diseases are not the main application targets of these three agents. On the other hand, validamycin A, which is also considered to have the ability to induce plant disease resistance, is registered as a control agent for soil diseases such as eggplant blight, but there are very few registered soil diseases. is there.

JP-A-6-24924 JP 2007-261992 A JP-A-6-41532 JP-A-55-40723 JP-A-10-248386 JP 2005-289945 A JP 2001-95382 A JP 2011-184370 A

Murakami, K., Shinoda, E., Maruta, R., Goto, Y. (2004) Control measures for broccoli clubroot by converter slag. Journal of Japanese Society of Soil Fertilizers 48: 608-613. Yoneyama Shingo (2011) Fusarium Classification, Ecology and Control. National Rural Education Association p. 282-286. Iwatsuki Yasuya (2012) Mitigation of Cucumber Damage by Improving Soil pH Using Converter Slag. Summary of Lectures on "Establishment of Comprehensive Control Technology for Cucurbitaceae Vegetable Homopsis Root Rot Corresponding to Damage Risk" p.16-19. Nakagawa Yasuo, Takagi Michinobu, Tsuji Yasuhiro, Nakamura Yoshihide, Kyoda Yukihiro (2002) Effects of sulfur materials on the occurrence of potato scab. Journal of Kyushu Pest 48: 18-23. Umemura, K., Tanino, S., Nagatsuka, T., Koga, J., Iwata, M., Nagashima, K., and Amemiya, Y. (2004) Cerebroside elicitor confers resistance to Fusarium disease in various plant species. Phytopathology 94: 813-818.

  Pesticides for disease control based on useful non-pathogenic microorganisms are available on the market, but they are not the main control measures because they are less effective and less stable than chemical agents. . On the other hand, soil pH correction can reduce some soil diseases, but its effect is only exerted when the degree of disease occurrence is low. Can not. In addition, resistance inducers reduce the incidence of soil diseases, but at the same time, it has been reported that the strength of effects and phytotoxicity are positively correlated, so it is dangerous to rely solely on them.

  In view of the above prior art, the object of the present invention is to effectively control soil-borne diseases regardless of soil sterilization or disease-resistant varieties, a soil-borne disease control method involving the promotion of crop growth, and the crops. It is an object of the present invention to provide a method for assaying useful microorganisms, a method for assaying a soil pH correction substance, and a method for assaying a plant disease resistance inducer, related to a method for controlling soil infectious diseases accompanied by growth promotion.

  The present inventors have intensively studied to achieve the above object, and have completed the present invention.

  The first aspect of the present invention is the growth of crops after planting in the field by applying useful microorganisms and plant disease resistance-inducing substances to soil or crops and applying soil pH correcting substances to soils during seeding and seedling periods of crops. The present invention provides a soil infectious disease control method that accompanies the promotion of the growth of crops, which is characterized by deriving the promotion and the effect of suppressing the occurrence of soil infectious diseases.

  According to the soil infectious disease control method with the growth promotion of the crop of the present invention, useful microorganisms and plant disease resistance inducers are added to the soil or the crop during the sowing / nurturing period of the crop, and the soil pH correcting material is cultured. Can be used to derive a synergistic disease-suppressing effect of useful microorganisms, soil pH-correcting substances, and plant disease resistance-inducing substances after field planting of crops, thereby effectively controlling soil-borne diseases. In addition, since it is accompanied by promotion of crop growth, it can contribute to shortening the cultivation period and increasing yield. Furthermore, since the usage-amount of a plant disease resistance inducer can be restrained, the risk of the phytotoxicity by it is also reduced.

  In the soil infectious disease control method accompanied by the growth promotion of the crop of the present invention, in the process of sowing, raising seedling, and fixed planting of the crop, the seedling that has sprouted is raised from the sowing bed to the pot, and then raised. When planting in the field, the useful microorganisms are applied to the sowing soil or the seed of the seed at the time of sowing until the time of potting, and further in the pot from the time of potting to the time of planting on the field. Reapply to the soil, and apply the soil pH correction material to the seeding soil during the period from seeding to pot raising, and again to the soil in the pot during the period from potting to planting in the field. Soil or crop foliage in the pot during the period from potting to planting in the field by applying the plant disease resistance-inducing substance to the soil only in the pot from potting to planting in the field It is preferable to give to

  In addition, when seedlings are planted directly from the sowing bed to the field without raising the pot during the sowing, raising, and planting of the crop, sowing soil or sowing during the period from seeding to planting of useful microorganisms To the seeds of the plant, soil pH correcting substance is applied to the sowing soil during the period from sowing to planting in the field, and plant disease resistance inducer is sowing soil during the period from sowing to planting in the field. It is preferable to apply to crop seeds at the time of sowing or to crop foliage during seedling raising.

  In addition, when seedlings are picked nutritionally without seeding, and then planted in the field after seeding, useful microorganisms are added to the soil during the period from seeding to planting, and a soil pH correction substance is collected. It is preferable to apply to the soil during the period from seedling to planting, and to apply the plant disease resistance-inducing substance to the soil during the period from seeding to planting or to the crop foliage during raising.

  On the other hand, according to the second aspect of the present invention, a test microorganism is applied to the soil or crop seed, a soil pH correcting substance is applied to the soil, a plant disease resistance inducer is applied to the soil or crop foliage, and then the soil habitat is added. When plant microbial pathogens are cultivated by adding them to soil or crop foliage, and as a result, the microorganisms under test tend to enhance the disease control effect by combining with soil pH-correcting substances and plant disease resistance inducers The present invention provides a method for assaying useful microorganisms, characterized in that the microorganisms are judged to be useful for the soil infectious disease control method involving the growth of crops according to the first invention.

  In addition, the test microorganisms are applied to the soil or crop seed, the soil pH correction substance is applied to the culture soil, and then the plant disease resistance inducer is applied to the soil or crop foliage and cultivated. Is a soil pH correction substance and a plant disease resistance inducer, when the growth of the crop is promoted, the soil infectious disease control method with the growth promotion of the crop according to the first aspect of the invention The present invention provides a method for assaying useful microorganisms, characterized in that the microorganisms are determined to be useful microorganisms.

  According to the method for assaying useful microorganisms of the present invention, when the soil pH correcting substance and the plant disease resistance inducer are determined to be predetermined ones, they are used in combination with them, and the soil infectious disease accompanying the growth promotion of crops is used. A microorganism useful for control can be easily screened from among a plurality of candidate microorganisms.

  In the third aspect of the present invention, useful microorganisms are imparted to the soil or crop seed, acidic or alkaline test substances are imparted to the soil, plant disease resistance inducers are imparted to the soil or crop foliage, and then soil habitat When the plant pathogenic fungi are applied to the soil or crop foliage and cultivated, and as a result, the tested acidic or alkaline substances tend to enhance the disease control effect by combining with useful microorganisms and plant disease resistance inducers Provides a soil pH correcting substance test method characterized in that it is determined to be a soil pH correcting substance useful for a soil infectious disease control method involving the growth promotion of the crop according to the first invention. is there.

  Also, useful microorganisms are applied to the soil or crop seeds, acidic or alkaline test substances are applied to the culture soil, and then plant disease resistance inducers are applied to the culture soil or crop foliage for cultivation. In the case where the growth of crops tends to be promoted by combining the acidic or alkaline substances with useful microorganisms and plant disease resistance inducers, soil infectivity with the promotion of crop growth according to the first aspect of the invention is described. The present invention provides a method for testing a soil pH correcting substance, characterized in that it is determined to be a soil pH correcting substance useful for a disease control method.

  According to the method for assaying a soil pH correcting substance of the present invention, when a useful microorganism and a plant disease resistance inducer are determined as predetermined ones, they are used in combination with them, and a soil infectious disease accompanied by the promotion of crop growth is used. A soil pH correcting substance useful for control can be easily screened from a plurality of candidate acidic substances or candidate alkaline substances.

  In the fourth aspect of the present invention, useful microorganisms are added to the soil or crop seed, a soil pH correcting substance is applied to the culture soil, a test substance is applied to the soil or crop foliage, and then the soil-habiting phytopathogenic fungi are cultured or When it is applied to the crop foliage and cultivated, and as a result, the tested substance shows a tendency to enhance the disease-inhibiting action by combining with a useful microorganism and a soil pH correcting substance, the crop of the first invention is used. The present invention provides an assay method for a plant disease resistance inducer characterized by determining that it is a plant disease resistance inducer useful for soil infectious disease control methods accompanied by growth promotion.

  Further, useful microorganisms are applied to the soil or crop seeds, soil pH-correcting substances are applied to the culture soil, and then the test substances are applied to the culture soil or crop foliage and cultivated. In the case of showing a tendency to promote the growth of crops by combining with a soil pH correction substance, a plant disease resistance inducer useful for the soil infectious disease control method involving the growth of crops according to the first aspect of the invention. The present invention provides a method for assaying a plant disease resistance inducer, characterized in that

  According to the plant disease resistance inducer testing method of the present invention, when useful microorganisms and soil pH correcting substances are determined to be predetermined ones, they are used in combination with them, and soil infectious diseases accompanied by crop growth promotion are used. A plant disease resistance inducer useful for control can be easily screened from a plurality of candidate substances.

  In the soil infectious disease control method with the growth promotion of the crop as the first invention, the useful microorganisms include Agrobacterium, Erwinia, Enterobacter, and Xanthomonas. (Xanthomonas), Gliocladium, Coniothyrium, Pseudomonas, Streptomyces, Talaromyces, Trichoderma, Burkholderia ( Burkholderia, Bacillus, Variovorax, Pythium, Fusarium, Penicillium, Lactobacillus, Rhizoctonia, mycorrhiza It is preferable to use one or more selected from fungi

  In addition, in the method for assaying useful microorganisms of the second aspect of the invention, one or more microorganisms whose disease-inhibiting action is enhanced by combining with a soil pH correcting substance and a plant disease resistance inducer, or the growth of crops is increased. It is preferable to use one or more microorganisms to be promoted as the useful microorganism.

  Further, as the soil pH correcting substance, converter slag, mashed lime, calcium hydroxide (slaked lime), calcium oxide (quick lime), calcium carbonate, ammonium sulfate, ammonium chloride, potassium sulfate, potassium chloride, sulfur flower, citric acid, It is preferable to use one or more selected from iron (II) sulfate and lime superphosphate.

  In addition, in the method for assaying a soil pH correcting substance according to the third aspect of the invention, one or two or more acidic or alkaline substances whose disease-inhibiting action is enhanced by combining with useful microorganisms and plant disease resistance inducers, or crops It is preferable to use one or more acidic or alkaline substances that promote the growth of the soil as the soil pH correcting substance.

  Further, as the plant disease resistance inducer, 1,3,5-benzenetricarboxylic acid, 2,2-dichloro-3,3-dimethylcyclopropanecarboxylic acid, 2,6-dichloroisonicotinic acid, 2-spotted Mucic acid, 3,4-dichlorobenzoic acid, 4-chlorobenzoic acid, 4-hydroxybenzoic acid, DL-tryptophan, D-alanine, D-galacturonic acid, D-glucuronic acid, D-phenylalanine, D-psicose, ImprimatinC1 , M-hydroxybenzoic acid, N-cyanomethyl-2-chloroisonicotinamide (NCI), WL 28325, α-aminoisobutyric acid, α-aminobutyric acid, β-aminobutyric acid (BABA), β-glucan, acifluorfen, acibenzoral -S-methyl, amino acid fermentation by-product solution, arachidonic acid, isotianil, isovanillic acid, nitric oxide, imidacloprid, eicosapentaenoic acid, ethylene, iron chloride, sodium chlorate, oite Extract, oleic acid, oregano extract, active oxygen, cabbage extract, carpropamide, kawaratake extract, chitin, chitosan, glycolic acid, glycine, glutamic acid, ketol fatty acids (carbon atoms 5-24), yeast extract, Salicylic acid, salicylic acid hydrazide, shiitake mycelium culture medium extract, dichloroisonicotinic acid (INA), jasmonic acid, oxalic acid, lipopolysaccharide derived from microorganisms of the genus Pseudomonas, syringic acid, shirotaegiku extract, spermine, cerebroside A, cerebroside B, Cerebroside C, cerebroside D, thiazinyl, thiamethoxam, dodecyl DL-alanine, dodecyl L-valine, mushroom extract, nigaritake extract, silicon dioxide, agaric extract, vanillic acid, sea pea extract, paraquat, parahydroxybenzoic acid Acid, validamycin A, validoxylamine A, hirohitake mushroom extract, phenanthroline, phthalocyanine, brassinosteroid, protocatechuic acid, prohydrojasmon, probenazole, phloroglucinol, mushroom extract, firefly bucuru extract, polyacrylic acid, matsuba button extract, It is preferable to use one or more selected from linoleic acid, linolenic acid, riboflavin, potassium phosphate, sodium phosphate, rose geranium extract, cotton jelly extract, and gallic acid.

  In addition, in the method for assaying a plant disease resistance inducer according to the fourth aspect of the present invention, one or more substances whose disease-inhibiting action is enhanced by combining with useful microorganisms and soil pH correcting substances, or the growth of crops is increased. It is preferable to use one or more kinds of substances to be promoted as the plant disease resistance inducer.

  In addition, the crop is melon, pumpkin, cucumber, watermelon, pickaxe, tougan, loofah, capsicum, chili pepper, tomato, eggplant, cauliflower, cabbage, komatsuna, chinsaisai, Chinese cabbage, broccoli, turnip, radish, wasabi, udo, Chrysanthemum, lettuce, celery, parsley, strawberry, asparagus, onion, leek, leek, sweet potato, ginger, carrot, rice, broad bean, soybean, okra, spinach, rose, hollyhock, bouvardia, godetia, stock, habutton, acidansera, crocus , Higashigi, Freesia, Alstroemeria, Almeria, Stachys, Gloxinia, Saintpaulia, Iwahiba, Ominaeshi, Sakuraran, Canna, Campanula, Kyodo, Aster, Gerbera, Gaza A, chrysanthemum, calendula, cosmos, zion, zinnia, cineraria, shasta daisy, shukkon aster, soridaster, dahlia, daisy, harshagiku, sunflower, pireokiku, fujibakama, safflower, margaret, marigold, miyakowasle, wheat wagtail, cornflower Rudbeckia, Aferrandra, Catharanthus, Anemone, Columbine, Clematis, Peonies, Prunus serrata, Dermatonium, Papaver, Fukujuso, Ranunculus, Poppy, Pteromia, Pepperidae, Chrysodium, Primula Ajuga, Kaktranoo, Salvia, Begonia, Water Lily, Porturaca, Violet, Why Lace flower, purple motomoto, spinach, petunia, physalis, carnation, gypsophila, red-crowned gypsophila, strelitzia, amaryllis, ukezakikun silane, daffodil, neline, hamaomoto, celosia, geranium, kalanchoe, sweet pea, lupine, allium, aloe, aloe, aloe , Omoto, Oryzul orchid, Hosta, Corticum, Thundersonia, Janobige, Tulip, Trubakia, Dracaena, Triteria, New Sairan, Haran, Hyacinth, Phototogis, Lily, Ruscus, Cypridella, Oncidium, Cattleya, Cymbidium, Dendrobium, Pepperbium, Paphidavir , Phalaenopsis, Miltonia, Exacam, Eustoma, Gentian, Poinsettia, Ma Ngo, oyster, gummy, fig, passion fruit, pineapple, papaya, apricot, plum, sweet cherry, quince, raspberry, plum, pear, pear, loquat, quince, peach, apple, grape, chestnut, kiwifruit, citrus It is preferable that it is 1 type.

Also, soil infectious diseases are melon vine split disease, melon necrotic spot disease, melon mosaic disease, melon brown spot bacterial disease, melon oncosis, melon soft rot, melon spot bacterial disease, melon hair root disease, melon epidemic , Melon sclerotia, melon red root rot, melon black spot rot, melon white silk, melon blight, melon vine blight, melon seed blight, melon root rot, melon root rot, Melon half body wilt, pumpkin bacterial wilt, pumpkin brown spot, pumpkin spot bacterial, pumpkin plague, pumpkin white silk, pumpkin wilt, pumpkin vine, cucumber green spot, cucumber green wilt, Cucumber brown spot bacterial disease, Cucumber soft rot, Cucumber spotted bacterial disease, Cucumber plague, Cucumber brown spot disease, Cucumber mycosis, Cucumber white silk disease, Cucumber vine blight, Cucumber vine split disease, Cucumber seedling blight, cucumber Li root rot, cucumber gray plague, cucumber half body wilt, cucumber homoposis root rot, cucumber purple crest, watermelon green spot mosaic disease, watermelon wilt bacterial disease, watermelon brown spot bacterial disease, watermelon plague, watermelon sclerotia , Watermelon black spot rot, watermelon white silk disease, watermelon vine blight, watermelon vine blight, watermelon vine split disease, watermelon half-wilt, watermelon fusarium blight, vine spotted bacterial disease, tougan vine disease, Togan vine Blight, Touhou vine split disease, Loofah vine split disease, Loofah rot rot, Loofah seedling blight, Yugao brown bacterial disease, Yugao spotted bacterial disease, Yugao black spot rot, Yugao white silk disease, Yugao vine wilt , Yugao vine split disease, Yugao seedling blight, Yugao gray plague, Pepper pepper green wilt, Pepper pepper sweet potato, Pepper pepper sweet rot, Pepper Pepper spot bacterial disease, pepper, pepper wilt, pepper pepper, plague, pepper pepper, nuclear disease, pepper pepper, black spot root rot, pepper pepper, white silkworm, pepper pepper pepper blight, pepper pepper pepper establishment Blight, pepper pepper half body wilt, tomato streak disease, tomato mosaic disease, tomato blight, tomato scab, tomato stem blight, tomato black blight, tomato soft rot, tomato spot bac, Tomato spotted bacterial disease, tomato rot, tomato alternaria stem blight, tomato wilt, tomato plague, tomato brown root rot, tomato brown rot, tomato mycosis, tomato red root rot, tomato black spot rot Disease, tomato scab, tomato silkworm, tomato seedling blight, tomato root rot, tomato root rot wilt, tomato root rot, tomato gray plague Disease, tomato half-wilt disease, eggplant mosaic disease, eggplant blight, eggplant brown bacterial disease, eggplant stem rot, eggplant stem rot, eggplant soft rot, eggplant spotted bacterial disease, eggplant plague, eggplant brown rot Disease, eggplant mycosis, eggplant black spot rot, eggplant white silk disease, eggplant seedling blight, eggplant root rot, eggplant half blight, eggplant half wilt, cauliflower black rot, cauliflower black spot bacterial disease, Cauliflower soft rot, cauliflower wilt, cauliflower root-knot, cabbage black rot, cabbage black spot bacterial disease, cabbage soft rot, cabbage wilt, cabbage strain rot, cabbage rot, cabbage root rot, cabbage root-knot Disease, cabbage verticillium wilt, cabbage seedling blight, komatsuna yellow wilt, chingensai spot bacterial disease, chingensai wilt, Chinese cabbage black rot, Chinese cabbage black spot bacterial disease, Chinese cabbage soft rot, Chinese cabbage rot disease Chinese cabbage yellow disease, Chinese cabbage rot, Chinese cabbage rot, Chinese cabbage root neck disease, Chinese cabbage root club disease, Chinese cabbage root rot, broccoli picium rot, broccoli picium rot, turnip blight, turnip black rot, turnip black spot bacterial disease , Turnip soft rot, turnip yellow rot, turnip root rot, turnip root rot, turnip root rot, turnip root rot, turnip root knot, turnip radish black spot, radish black rot, radish black rot , Japanese radish black spot ring rot, Japanese radish black spot bacterial disease, Japanese radish soft rot, Japanese radish yellow rot, Japanese radish round brown spot disease, Japanese radish mycosis, Japanese radish black spot disease, Japanese radish root rot, Japanese radish root knot Disease, radish leaf rot, radish black spot disease, radish rot, radish blight, wasabi sclerotia, wasabi stem rot, horseradish sump disease, wasabi root-knot disease, Udo wilt disease, udo Gonorrhea, powdery mildew, powdery mildew, powdery white silkworm, common scab, sunggi bacterial wilt, sungyk black rot, sungyk rot, sungyk wilt, sungyk nuclear disease, lettuce soft rot, lettuce spots Bacterial disease, lettuce rot disease, lettuce sclerotia, lettuce granule nuclei disease, lettuce root blight, lettuce root rot, celery soft rot, celery leaf blight, celery leaf blight, celery rot, celery wilt , Celery sclerotia, parsley soft rot, parsley wilt, parsley wilt, parsley wilt, parsley seedling, parsley root rot, parsley root rot, strawberry yellow spot, strawberry wilt, strawberry wilt Disease, strawberry plague, strawberry rot, strawberry mycosis, strawberry black root, strawberry white silk, strawberry soft rot, strawberry root rot, strawberry sprout, asparagus brown rot, aparagus Sparagus strain rot, asparagus white rot, asparagus wilt, asparagus seed wilt, asparagus purple rot, onion scab, onion soft rot, onion spot bacterial disease, onion rot, onion Onion rot, onion plague, onion dry rot, onion fungus nuclear disease, onion black mold, onion black rot fungus, onion smut, onion red root rot, onion sclerotia nuclear disease, onion silkworm, Onion white plague, onion seedling blight, leek strain rot, leek soft rot, leek dry rot, leek black rot nuclear disease, leek red root rot, leek white silkworm, leek white rot, leek leaf rot Disease, leek spotted disease, leek soft rot, leek spotted bacterial disease, leek rot disease, leek wilt disease, leek plague, leek black rot nuclear disease, leek smut, leek red root rot, leek sclerotia nuclear disease, leek White silkworm, leek white plague, leek seedling blight, Sweet potato wilt, sweet potato blue mold, sweet potato scab, sweet potato brown dry rot, sweet potato nuclear disease, sweet potato black rot, sweet potato black spot disease, sweet potato microgravity, sweet potato white silk disease, sweet potato white rot, Sweet potato white rot, sweet potato charcoal, sweet potato vine split, sweet potato soft rot, sweet potato root rot, sweet potato gray mold, sweet potato purple crest, ginger rot, ginger rhizome rot, ginger wilt, Ginger blight, carrot ankle, carrot root carcinoma, carrot streptomyces scab, carrot soft rot, carrot spotted bacterial disease, carrot yellow, carrot brown root rot, carrot dry rot, carrot fungus Nuclear disease, carrot black soot, carrot black root rot, carrot spot rot, carrot white silkworm, carrot scab Carrot root rot, carrot purple rot, rice rice mildew, rice plague, rice strain rot, rice leaf blight, rice seedling bacterial disease, rice foliage bacterial disease, rice plague, rice brown sclerotia , Rice brown sclerotia, rice brown rot, rice cocci, rice black sclerotia, rice black sclerotia, rice staphylococcal nuclei, rice silkworm, rice red sclerotia, Rice Blight, Rice Seed Blight, Rice Seed Blight, Rice Gray Bacterial Disease, Rice Blast Seed Disease, Rice Leaf Sheath Blight, Rice Blight, Rice Cotton Blight, Broad Bean Blight, Broad Bean Blight, Broad bean nuclear disease, broad bean stalk rot, broad bean black root disease, broad bean white silk disease, broad bean white rot, broad bean root rot, broad bean root rot, soybean leaf spotted leaf disease, soybean leaf spot disease, soybean leaf burning disease , Soybean spot bacterial disease, soybean wilt disease, soybean strain blight, soybean sclerotia, soybean stem blight, soybean black root disease , Soybean black root rot, Soybean white rot, Soybean stem blight, Soybean rhizoctonia root rot, Okra blight, Okra blight, Okra half wilt, Spinach mosaic disease, Spinach wilt, Spinach blight, Spinach strain rot , Spinach mushroom disease, spinach blight, spinach verticillium wilt, spinach root rot, rose head cancer, rose blight, rose half body wilt, hollyhock white silkworm, bouvardia seedling blight, Godetia wilt, stock black rot, stock wilt, stock plague, stock strain disease, stock wilt, stock seed rot, stock seed wilt, stock half wilt, ha button wilt, acidan serra heart rot , Acidan Serra soft rot, Acidan Serra white silk disease, Acidi Serrato Tritis rhizome rot, Acidan Serra dry rot, Sidansera strain disease, acidansera white silk disease, crocus soft rot disease, crocus dry rot disease, crocus bulbous sclerosis disease, crocus bulbous rot disease, crocus sclerotia disease, Huanggi silkworm disease, freesia neck rot disease, freesia bulb rot disease, freesia bacterium Nuclear Disease, Freesia Standing Disease, Alstroemeria Plague, Alstroemeria Nuclear Disease, Alstroemeria White Silk Disease, Almeria White Silk Disease, Statice Blight, Statice Dwarf Bacterial Disease, Statice Disease, Statice Strains Disease, Gloxinia Plague, saintpaulia plague, scabbard silkworm, half body wilt disease, cherry blossom soft rot, canna stem rot, campanula sclerotia, campanula white silkworm, campanula root rot, Japanese radish blight, aster wilt, aster Blight, gerbera plague, gerbera nuclear disease, gerbera white Disease, gerbera root rot, gerbera half body wilt, gerbera spotted bacterial disease, gazania mycosis, chrysanthemum bacterial wilt, chrysanthemum root cancer, chrysanthemum rot, chrysanthemum wilt, chrysanthemum disease, chrysanthemum disease Chrysanthemum silkworm, chrysanthemum blight, chrysanthemum wilt, chrysanthemum stem blight, chrysanthemum white rot, calendula epidemic, calendula nuclear disease, calendula half body wilt, cosmos stem blight, cosmos half body wilt, Zion white silk disease, Zinnia wilt disease, Zinnia soft rot disease, Zinnia spotted bacterial disease, Zinnia sclerotia, Zinnia blight disease, Cineraria seedling blight, Shasta daisy half body wilt disease, Shukukonaster white silk disease, Shukukon Aster spot disease, soridaster white silk disease, dahlia blight disease, dahlia root cancer disease, dahlia soft rot disease, dahlia mycosis, dahlia white silk disease, dahlia seed blight, daisy fungus disease, harshagik blue Blight, sunflower wilt Disease, Sunflower Bacterial Disease, Sunflower Spot Bacterial Disease, Sunflower Bacterial Disease, Sunflower White Silk Blossom, Sunflower Seedling Blight, Pireogiku Mycosis, Fujibaka White Silk Blossom, Safflower Soft Rot, Safflower Spoilage, Margaret Blue Blight, Margaret Root Cancer, Margaret Dwarf, Margaret Bacterial Disease, Marigold Blight, Marigold Strain, Miyakowase Root Cancer, Miyakowase Dwarf, Miyakowase Epidemic, Miyakowase Straw Disease, Miyakowa white silk disease, wheat waft half body wilt disease, corn sclerotia nuclear disease, corn corn white rot, ria tris karyopathia, ria tris white silk disease, rudbeckia wilt disease, rudbeckia white silk wilt, rudbeckia half body wilt disease, afelandra plague, periwinkle Dwarf yellow, periwinkle plague, periwinkle nuclear disease, periwinkle seedling blight, anemo Plague, anemone bulb rot, anemone nuclear disease, aquilegia white silk disease, clematis root cancer disease, clematis white silk disease, peonies root cancer disease, peonies plague disease, peonies fungus nuclear disease, peonies white silk disease , Peony Blight, Cryptomeria japonica, Silkworm, Silkworm rot, Delphinium soft rot, Delphinium wilt, Delphinium wilt, Hanabori butterfly wilt, Hanabori beetle spot, Fukujuso rot fungus, ranunculus Bacterial disease, poppy wilt disease, poppy soft rot, poppy spotted bacterial disease, poppy sclerotia, poppy white coat, poppy head blight, poppy seed wilt, red-footed root rot , Peperomia white rot, Peperomia rot, Calceolaria seedling blight, Snapdragon spotted bacterial disease, Snapdragon plague, Snapdragon sclerotia, Snapdragon Stem rot, Snapdragon sclerotia, Snapdragon root rot, Snapdragon root rot, Snapdragon half wilt, Primula soft rot, Primula leaf blight, Primula rot, Primrose wilt, Primula spot, Cyclamen Soft rot, Cyclamen wilt, Cyclamen seed rot, Cyclamen seedling blight, Color soft rot, Color plague, Collar white silk disease, Karadium mycosis, Caradium white silk disease, Philodendon plague, Cactus blight, Cactus stem blight , Cactus stalk rot, cactus rot, ajuga white silk, kattoranoo white scab, salvia epidemic, begonia bacterial spot disease, begonia stalk rot, water lily spotted rot, portulaca rot, violet root rot, white Lace flower wilt, white lace flower plague, white lace flower withering disease, purple peach Plague, spinach rot, spinach plague, spinach white silk disease, spinach wilt disease, petunia nuclear disease, petunia mildew, physalis spotted bacterial disease, physalis white silk disease, physalis half body wilt disease, carnation wilt bacterial disease, Carnation Bacterial Disease, Carnation Spotted Bacterial Disease, Carnation Dwarf Disease, Carnation Bacterial Disease, Carnation Bacterial Disease, Carnation Stem Rot, Carnation Neck Rot, Carnation White Silk Blossom, Carnation Blight, Carnation Root Disease, Gypsophila , Gypsophila nuclear disease, Gypsophila wilt bacterial disease, Gypsophila knot disease, Gypsophila root cancer, Gypsophila spot bacterial disease, Gypsophila epidemic, Gypsophila stem rot, Gypsum Gypsophila blight Gypsophila seedling rot, Strelitzia bacterial wilt, Strelitzia plague, Amaryllis plague, Ukezakikun silane silkworm, Narcissus soft rot, Narcissus dry rot, Narcissus bulb rot, Narcissus nuclear disease, Narcissus white rot, Nerine plague, Neline white silkworm, Hamamoto white silkworm, Celosia plague, Celosia blight, Celosia root rot, Geranium stem rot, Geranium blight, Geranium spot disease, Kalanchoe plague, Sweet pea wilt, Sweet pea fungus Nuclear disease, sweet pea half-wilt disease, lupine wilt disease, lupine fungus nuclear disease, lupine white silk disease, lupine white spotted blight, lupine blight, allium plague, aloe plague, ornithogalum plague, omoto baldness, hosta White silkworm, corticum root rot, sandersonia white silk disease, sandersonia wilt, janoge white silkworm, Tulip black rot, tulip soft rot, tulip blue mold, tulip plague, tulip bulb rot, tulip bulb rot, tulip mycosis, tulip stem blight, tulip white silk disease, tulip white rot, tulip gray rot, Tulip root rot, Tulip rot, Turbakia silkworm, Dracaena plague, Triteria plague, New sairan silkworm, Haran nuclear disease, Hyacinth soft rot, Hyacinth rot, Hyacinth nuclear disease, Hyacinth silkworm, Hyacinth white Plague, white scab, lily soft rot, lily blue mold, lily pesticidal, black lichen black rot nuclear disease, lily white silkworm, lily purple crest, ruscus spot disease, red scab Cypridina rot, Oncidium soft rot, Oncidium plague, Cattleya Brown rot, cattleya brown spot, cattleya plague, cattleya soft rot, cattleya seed black rot, cymbidium brown rot, cymbidium soft rot, cymbidium plague, cymbidium brown leaf blight, cymbidium white silk, cymbidium seed black rot Disease, cymbidium rot, dendrobium brown rot, dendrobium soft rot, dendrobium white silk disease, dendrobium seed rot, dendrobium rot, paphiopedilum brown rot, paphiopedilum soft rot, banda brown rot, vanda soft rot, banda plague , Banda silkworm, Bilstekera brown rot, Phalaenopsis brown rot, Phalaenopsis brown bacterial disease, Phalaenopsis soft rot, Phalaenopsis silkworm, Phalaenopsis strain blight, Miltonia brown rot, Exazacam strain blight, Eustoma wilt , Toru Gypsophila rot, Eustoma nuclei, Eustoma stem rot, Eustoma root rot, Eustoma root rot, Gentian leaf spot, Gentian brown root rot, Gentian white silk, Gentian leaf rot, Gentian leaf rot fungus Disease, gentian gonorrhea, poinsettia root rot, mango root cancer, oyster root cancer, oyster white herb, oyster purple herb, oyster homoposis blight, gummy white herb , Gummy micronucleus nuclear disease, fig head cancer, fig epidemic, fig fungal nucleus disease, fig white silkworm, fig white coat rot, fig soft rot, fig purple rot, passion fruit epidemic, passion fruit Mycorrhizal disease, pineapple flower rot, pineapple heart rot, papaya root cancer disease, papaya seedling blight, papaya soft rot disease, apricot root cancer disease, apricot white crest feather disease, anne Purple herb disease, ume root cancer disease, ume blight disease, ume fungus nuclear disease, ume blossom coat disease, ume purple herb disease, sweet potato root cancer disease, sweet potato nuclear disease, sweet cherry baldness Disease, sweet cherry blossoms, karin baldness, raspberry root cancer, plum root cancer, plum baldness, plum purple baldness, pear plague, pear white crest Feather disease, pear root cancer disease, pear plague disease, pear sclerotia disease, pear white coat feather disease, pear purple coat disease, loquat root cancer disease, loquat epidemic disease, loquat white coat feather disease, loquat purple Hermaphroditis, Quince root cancer disease, Peach root cancer disease, Peach fungus nuclear disease, Peach baldness, Peach purple coat disease, Apple root cancer disease, Apple blight, Apple white Silk disease, apple baldness, apple purple baldness, grape head cancer, grape baldness, grape half-wilt, grape purple baldness, chestnut root cancer Scab, chestnut plague, chestnut white coat, chestnut purple coat, kiwi fruit white coat, citrus root cancer, citrus nuclear disease, citrus white coat, citrus purple coat, It is preferably at least one selected from citrus Fusarium wilt.

  According to the soil infectious disease control method with the growth promotion of the crop of the present invention, useful microorganisms and plant disease resistance inducers are added to the soil or the crop during the sowing / nurturing period of the crop, and the soil pH correcting material is cultured. Can be used to derive a synergistic disease-suppressing effect of useful microorganisms, soil pH-correcting substances, and plant disease resistance-inducing substances after field planting of crops, thereby effectively controlling soil-borne diseases. In addition, since it is accompanied by promotion of crop growth, it can contribute to shortening the cultivation period and increasing yield. Furthermore, since the usage-amount of a plant disease resistance inducer can be restrained, the risk of the phytotoxicity by it is also reduced.

  According to the method for assaying useful microorganisms of the present invention, when the soil pH correcting substance and the plant disease resistance inducer are determined to be predetermined ones, they are used in combination with them, and the soil infectious disease accompanying the growth promotion of crops is used. A microorganism useful for control can be easily screened from among a plurality of candidate microorganisms.

  According to the method for assaying a soil pH correcting substance of the present invention, when a useful microorganism and a plant disease resistance inducer are determined as predetermined ones, they are used in combination with them, and a soil infectious disease accompanied by the promotion of crop growth is used. A soil pH correcting substance useful for control can be easily screened from a plurality of candidate acidic substances or candidate alkaline substances.

  According to the plant disease resistance inducer testing method of the present invention, when useful microorganisms and soil pH correcting substances are determined to be predetermined ones, they are used in combination with them, and soil infectious diseases accompanied by crop growth promotion are used. A plant disease resistance inducer useful for control can be easily screened from a plurality of candidate substances.

It is a graph which shows the result of the main stem length above the cotyledon node of the melon in Experimental example 2 (field experiment). It is a graph which shows the result of the raw fruit weight of the melon in Experimental example 2 (field experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of melon in Experimental example 3 (indoor small-scale experiment). It is a graph which shows the result of the number of nodes when the main stem length above a cotyledon node of a melon and the cotyledon node are made into the 1st node in Experimental example 4 (net room experiment). It is the photograph which image | photographed the photograph of the melon plant 41 days after transplanting in the first experiment of Experimental example 4 (net room experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of the tomato in Experimental example 5 (indoor small-scale experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of the tomato in Experimental example 6 (indoor small-scale experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of a tomato in Experimental example 7 (indoor small-scale experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of a tomato in Experimental example 8 (indoor small-scale experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of melon in Experimental example 9 (indoor small-scale experiment). It is a graph which shows the result of the production amount of the plant body above the cotyledon node of the cabbage in Experimental example 10 (indoor small-scale experiment).

  The useful microorganism used in the soil infectious disease control method accompanied by the growth promotion of the crop of the present invention refers to a microorganism useful for controlling the soil infectious disease. For example, Agrobacterium genus (Agrobacterium), Erwinia genus (Erwinia), Enterobacter genus (Xanthomonas), Gliocladium genus (Gliocladium), Coniothyrium genus (Pseudomonas), Streptomyces genus, Talaromyces genus, Trichoderma genus, Burkholderia genus, Bacillus genus, Variovorax genus, Pythium genus, Fusarium genus Examples include microorganisms belonging to (Fusarium), Penicillium, Lactobacillus, Rhizoctonia, mycorrhizal fungi and the like. In particular, Agrobacterium radiobacter, Bacillus subtilis, Bacillus thuringiensis, Burkholderia cepacia, Coniothyrium minitans, Enterobacter cloacae, non-pathogenic Erwinia carotovora subsp. Carotovora, Erwinia herbicola, Fusarium equiseti, Fusarium equisetiporum Fusarium solani, Lactobacillus kyotoensis, Lactobacillus plantarum, Penicillium ozalicum ( Penicillium oxalicum, Pseudomonas cepacia, Pseudomonas fluorescens, Pseudomonas putida, Pythium oligandrum, Pythium athophorium Pythium mycoparasiticum, Binucleate Rhizoctonia sp., Streptomyces galbus, Talaromyces flavus, Tricoderma atrovitrima harzianum, Trichoderma viride, Variovorax paradoxus, Xanthomonas campestris, Xanthmona Maltophilia (Xanthomonas maltophilia) include the microorganisms belonging to such.

  As useful microorganisms, those already reported as useful microorganisms for controlling soil-borne diseases can be used, and obtained by screening from the microorganisms tested for them using the useful microorganism assay method described below. May be used. In particular, Fusarium oxysporum SL0037 strain (Fusarium oxysporum SL0037 strain, National Institute for Product Evaluation and Technology Patent Microorganism Deposit Center accession number: NITE P-01629) has a control effect on soil infectious diseases as shown in the Examples below. Since the effect of promoting crop growth is high, it can be preferably used.

  One useful microorganism may be used alone, or two or more may be used in combination.

  In Tables 1 and 2 below, microorganisms that have been reported as microorganisms useful for controlling soil-borne diseases and their literatures are listed.

  The soil pH correcting substance used in the soil infectious disease control method accompanied by the growth promotion of the crop of the present invention refers to a substance that is added to culture soil to adjust its pH. For example, converter slag, mashed lime, calcium hydroxide (slaked lime), calcium oxide (quick lime), calcium carbonate, ammonium sulfate, ammonium chloride, potassium sulfate, potassium chloride, sulfur flower, citric acid, iron (II) sulfate, excess Examples thereof include lime phosphate.

  As the soil pH correcting substance, a substance obtained by screening from an acidic or alkaline substance used for the test according to the soil pH correcting substance test method described later may be used.

  One kind of soil pH correcting substance may be used alone, or two or more kinds may be used in combination.

  The plant disease resistance inducer used in the soil infectious disease control method with growth promotion of the crop of the present invention refers to a substance that induces disease resistance inherent in plants. For example, 1,3,5-benzenetricarboxylic acid, 2,2-dichloro-3,3-dimethylcyclopropanecarboxylic acid, 2,6-dichloroisonicotinic acid, 2-pyromycinous acid, 3,4-dichlorobenzoic acid Acid, 4-chlorobenzoic acid, 4-hydroxybenzoic acid, DL-tryptophan, D-alanine, D-galacturonic acid, D-glucuronic acid, D-phenylalanine, D-psicose, ImprimatinC1, m-hydroxybenzoic acid, N- Cyanomethyl-2-chloroisonicotinamide (NCI), WL 28325, α-aminoisobutyric acid, α-aminobutyric acid, β-aminobutyric acid (BABA), β-glucan, acifluorfen, acibenzoral-S-methyl, amino acid fermentation by-product Liquid, arachidonic acid, isotianil, isovanillic acid, nitric oxide, imidacloprid, eicosapentaenoic acid, ethylene, iron chloride, sodium chlorate, Euphorbi extract, oleic acid, oregano extract , Active oxygen, cabbage extract, carpropamide, kawaratake extract, chitin, chitosan, glycolic acid, glycine, glutamic acid, ketol fatty acids (5 to 24 carbon atoms), yeast extract, salicylic acid, salicylic acid hydrazide, shiitake mycelium Culture medium extract, dichloroisonicotinic acid (INA), jasmonic acid, oxalic acid, Lipopolysaccharide from Pseudomonas microorganisms, syringic acid, Shirotaegiku extract, spermine, cerebroside A, cerebroside B, cerebroside C, cerebroside D, thiazinyl, Thiamethoxam, dodecyl DL-alanine, dodecyl L-valine, scented mushroom extract, nigaritake extract, silicon dioxide, agaric extract, vanillic acid, peanut extract, paraquat, parahydroxybenzoic acid, validamycin A, validoxy Min A, Hirohitake mushroom extract, phenanthroline, phthalocyanine, brassinosteroid, protocatechuic acid, prohydrojasmon, probenazole, phloroglucinol, dust mushroom extract, firefly extract, polyacrylic acid, matsuba button extract, linoleic acid, linolenic acid Riboflavin, potassium phosphate, sodium phosphate, rose geranium extract, cotton jelly extract, gallic acid and the like. In general, the plant disease resistance inducer is contained as an active ingredient in the plant resistance inducer.

  As the plant disease resistance inducer, those already reported as useful substances for inducing disease resistance inherent in plants may be used, and the plant disease resistance inducer test method described later is also used. According to the above, a substance obtained by screening from the substances used in the test may be used.

  The plant disease resistance inducer may be used alone or in combination of two or more.

  Tables 3 and 4 below list substances that have been reported as useful substances for inducing disease resistance inherent in plants, and the basis documents thereof.

  The soil infectious disease control method with growth promotion of the crop of the present invention uses the above-mentioned useful microorganisms, the soil pH correcting substance and the plant disease resistance-inducing substance in combination at the sowing / nurturing period or the seedling period of the crop. It is characterized by that. In other words, the useful microorganisms and soil after planting of the crops in the field by applying the useful microorganisms and plant disease resistance-inducing substances to the soil or the crops and the soil pH correcting substance to the soil during the sowing / nurturing stage of the crops. It is characterized by deriving a synergistic disease-suppressing effect of a pH-correcting substance and a plant disease resistance-inducing substance and an effect of promoting crop growth that accompanies it.

  For example, in the process of sowing, raising seedlings, and planting of crops, when raising seedlings by transplanting the germinated seedlings from the seeding floor to pots, and then planting them in the field, potting the useful microorganisms from the time of sowing This is applied to the sowing soil during the period until sowing or the crop seed at the time of sowing, and is also applied again to the soil in the pot during the period from potting to planting in the field. It is given to the sowing soil during the period until it is raised, and it is also given again to the soil in the pot during the period from potting to planting in the field, or the pot during the period from potting to planting in the field. Soil infectivity after planting of the crop in the field by applying the plant disease resistance inducer to the soil or crop foliage in the pot during the period from potting to planting in the field. Control disease and promote growth It can be.

  Also, for example, in the case of planting seedlings directly from the seeding floor to the field without raising the pot in the process of sowing, raising seedlings, and planting of the crop, sowing soil or soil for the period from seeding to planting Giving to crop seeds at the time of sowing, applying soil pH-correcting substances to the sowing soil during the period from sowing to planting in the field, and sowing for the period from planting to planting the plant disease resistance inducer By applying to soil or seeds of crops at the time of sowing or crop stalks and leaves of seedlings, it is possible to control soil-borne diseases after field planting of the crops and promote growth.

  Furthermore, for example, when seedlings are picked nutritionally without seeding, and planted in the field after seeding, useful microorganisms are added to the soil during the period from seeding to planting, and a soil pH correction substance Is applied to the soil during the period from seeding to planting, and the plant disease resistance inducer is applied to the soil during the period from seeding to planting or to the crop foliage during the seedling raising. It is possible to control the soil infectious diseases and promote the growth.

  Application of useful microorganisms, soil pH-correcting substances, and / or plant disease resistance-inducing substances to the seeding soil or culture soil can be carried out by mixing, irrigating, or spraying the soil. Application of useful microorganisms and / or plant disease resistance inducers to crops, crop seeds, or crop foliage can be carried out by spraying, applying, dipping, or injecting the crops.

  The crop to which the method for controlling soil infectious diseases accompanied by the growth promotion of the crop of the present invention is not particularly limited, for example, melon, pumpkin, cucumber, watermelon, vine moth, tougan, loofah, yugao, chili pepper, bell pepper, Tomato, eggplant, cauliflower, cabbage, komatsuna, Chinese cabbage, Chinese cabbage, broccoli, turnip, Japanese radish, horseradish, udo, shungiku, lettuce, celery, parsley, strawberry, asparagus, onion, leek, leek, sweet potato, ginger, carrot, rice , Broad bean, soybean, okra, spinach, rose, hollyhock, bouvargia, godetia, stock, habutton, acidansera, crocus, cypress, freesia, alstroemeria, almeria, staticis, gloxinia, saintpaulia, iwahiba, Minaeshi, Sakuraran, Kanna, Campanula, Kyoukyo, Aster, Gerbera, Gazania, Chrysanthemum, Calendula, Cosmos, Zion, Zinnia, Cinearia, Shasta Daisy, Shuku Conaster, Solida Star, Dahlia, Daisy, Harshagik, Sunflower, Pireogiku, Fujibakabe , Margaret, Marigold, Miyakowase, Wheatwagiku, Cornflower, Liatris, Rudbeckia, Afelandra, Catharanthus, Anemone, Odamaki, Clematis, Peonies, Shumeigi, Suhamasou, Delphinium, Hanafikabuso Snapdragon, primrose, cyclamen, color, caradium, philodendon, cactus, a Yuga, Kaktoranoo, Salvia, Begonia, Water Lily, Porturaca, Violet, White Lace Flower, Murasaki Omoto, Spinach, Petunia, Physalis, Carnation, Gypsophila, Gypsophila Gypsophila, Strelitzia, Amaryllis, Ukezakikun silane, Narcissus, Nerine, Hamaomoto , Celosia, geranium, kalanchoe, sweet pea, lupine, allium, alony, ornithogalum, omoto, oryzul orchid, giboshi, colchicum, sandersonia, janohige, tulip, trubakia, dracaena, triteria, new sairan, haran, hyacinth, lily , Cypridina, Oncidium, Cattleya, Cymbidium, Dendrobium, Paphiopedilum, Vanda, Birstekera, Fu Arenopsis, miltonia, exacam, eustoma, gentian, poinsettia, mango, oysters, gummy, figs, passion fruit, pineapple, papaya, apricot, ume, sweet cherry, quince, raspberry, plum, pear, pear, loquat, quince, peach, Examples include apples, grapes, chestnuts, kiwi fruits, and citrus.

The soil infectious diseases to which the method for controlling soil infectious diseases accompanied by the growth promotion of the crops of the present invention is applied are not particularly limited, for example, melon vine split disease, melon necrotic spot disease, melon mosaic disease, melon brown Spot bacterial disease, melon oncosis, melon soft rot, melon spot bacterial disease, melon hair root disease, melon epidemic, melon sclerotia, melon red root rot, melon black spot rot, melon white silk disease, melon standing Blight, melon vine blight, melon seed rot, melon root rot, melon root rot wilt, melon half wilt, pumpkin blight, pumpkin brown bacterial disease, pumpkin spot bacterial disease, pumpkin plague, pumpkin White silk disease, pumpkin blight, pumpkin vine blight, cucumber green spot mosaic disease, cucumber green blight, cucumber brown bacterial disease, cucumber soft rot, cucumber spot bacterial disease, cucumber blight, cucumber brown spot, cucumber Mycorrhizal disease, cucumber white silkworm, cucumber vine blight, cucumber vine split disease, cucumber seedling blight, cucumber root rot, cucumber gray plague, cucumber half wilt, cucumber homoposis root rot, cucumber purple crest disease , Watermelon green spot mosaic disease, watermelon wilt bacterial disease, watermelon brown spot bacterial disease, watermelon epidemic, watermelon mycosis, watermelon black spot rot, watermelon white silkworm, watermelon stand blight, watermelon vine blight, watermelon vine split Disease, watermelon half-wilt disease, watermelon Fusarium blight, vine spotted bacterial disease, Tougan blight, Togan vine blight, Togan vine split disease, Loofah vine split disease, Loofah rot, Loofah seedling wilt, Yugao Brown spotted bacterial disease, Yugao spotted bacterial disease, Yugao black spot rot, Yugao silkworm, Yugao vine wilt, Yugao vine split disease, Yugao seedling wilt, Yugao gray plague, Pepper pi Man wilt, pepper, pepper pepper, soft pepper, soft pepper, pepper pepper spot bacterial disease, pepper pepper wilt, pepper pepper pepper disease, pepper pepper pepper nuclear disease, pepper pepper black spot rot, Pepper and pepper green rot, Pepper and pepper pepper wilt, Pepper and pepper pepper seedling, Pepper and pepper half body wilt, tomato streak, tomato mosaic disease, tomato bacterial wilt, tomato stem disease, tomato stem Bacterial disease, tomato black spot bacterial disease, tomato soft rot, tomato spotted bacterial disease, tomato spotted bacterial disease, tomato rot, tomato alternaria stem blight, tomato wilt, tomato plague, tomato brown root rot, tomato Brown rot, tomato sclerotia, tomato safflower root rot, tomato black spot rot, tomato granule nuclei, tomato White silkworm, tomato seedling blight, tomato root rot, tomato root rot wilt, tomato root rot, tomato gray wilt, tomato half wilt, eggplant mosaic disease, eggplant blight, eggplant brown spot disease, Eggplant stem rot, eggplant stem rot, eggplant soft rot, eggplant spotted bacterial disease, eggplant plague, eggplant brown rot, eggplant sclerotia, eggplant black spot rot, eggplant white silk disease, eggplant seedling wilt Disease, eggplant root rot, eggplant half blight, eggplant half wilt, cauliflower black rot, cauliflower black rot, cauliflower soft rot, cauliflower yellow rot, cauliflower root club, cabbage black rot, cabbage black rot Disease, cabbage soft rot, cabbage wilt, cabbage strain rot, cabbage root rot, cabbage root rot, cabbage root-knot, cabbage verticillium wilt, cabbage seedling blight, komatsuna wilt, spotted rhinoceros Disease, chi Gensai yellow rot, Chinese cabbage black rot, Chinese cabbage black spot bacterial disease, Chinese cabbage soft rot, Chinese cabbage rot disease, Chinese cabbage yellowing disease, Chinese cabbage fungus nuclear disease, Chinese cabbage rot, Chinese cabbage root constriction disease, Chinese cabbage root-knot disease, Chinese cabbage disease Rot, broccoli peptic rot, turnip blight, turnip black rot, turnip black spot blight, turnip soft rot, turnip yellow rot, turnip root disease, turnip root rot, turnip root rot, turnip root constriction Disease, turnip root-knot disease, turnip verticillium black spot disease, Japanese radish black rot, Japanese radish black rot, Japanese radish black spot rot, Japanese radish black spot bacterial disease, Japanese radish common scab, Japanese radish soft rot, Japanese radish wilt disease, Japanese radish Round brown spot disease, Japanese radish sclerotia, Japanese radish black spot, Japanese radish root rot, Japanese radish root rot, Japanese radish leaf rot, Japanese radish black spot disease, Japanese radish rot, Japanese radish rot Blight, horseradish nuclear disease, horseradish stem rot, horseradish ink disease, horseradish root-knot disease, powdery yellow rot, powdery mildew, powdery mildew, powdery mildew, powdery white silkworm, powdery mildew, Sengik wilt, Shungyoku rot, Sengiku rot, Sengiku wilt, Sengiku rot, lettuce soft rot, lettuce bacteriopathies, lettuce rot, lettuce rot, lettuce granulosis, lettuce rot Disease, lettuce root rot, celery soft rot, celery spotted bacterial disease, celery leaf bacterial disease, celery rot disease, celery wilt disease, celery sclerotia, parsley soft rot, parsley wilt disease, parsley epidemic, parsley wilt disease , Parsley seedling blight, parsley root rot, parsley root rot, strawberry keratosis, strawberry wilt, strawberry wilt, strawberry plague, strawberry rot, strawberry sclerosis, strawberry black root rot, B Chigo white silk disease, strawberry soft rot, strawberry root rot, strawberry bud blight, asparagus brown fungus root rot, asparagus strain rot, asparagus white coat rot, asparagus blight, asparagus seedling Blight, Asparagus purple crest, onion scab, onion soft rot, onion spot bacterial disease, onion rot, onion hemi rot, onion plague, onion dry rot, onion sclerotia, onion black mold , Onion black rot fungus, onion scab, onion red root rot, onion sclerotia, onion white silkworm, onion white plague, onion seedling blight, leek strain rot fungus, leek soft rot, leek Dry rot, leek black rot, nuclear rot, leek white silkworm, leek white plague, leek leaf rot, leek leaf spot, leek soft rot, leek spot bacterial disease, leek rot, leek wilt Disease, leek plague, leek black rot nuclear disease Leek smut, leek red root rot, leek sclerotia, leek white silkworm, leek white plague, leek seedling blight, sweet potato blight, sweet potato blue mold, sweet potato scab, sweet potato brown dry rot, Sweet potato nuclear disease, sweet potato black rot, sweet potato black spot disease, sweet potato granule sclerosis, sweet potato white silk disease, sweet potato white rot, sweet potato white coat, sweet potato charcoal disease, sweet potato vine split disease, sweet potato soft rot disease , Sweet potato root rot, sweet potato gray mold, sweet potato purple crest, ginger rot, ginger rhizome rot, ginger blight, ginger rot, carrot knot, carrot root cancer, carrot strep Mrs. common scab, carrot soft rot, carrot spot bacterial disease, carrot yellow rot, carrot brown root rot, carrot dry rot, carrot sclerotia, nin Black soot disease, carrot black root rot, carrot stain rot, carrot white silkworm, carrot scab, carrot root rot, carrot purple rot, rice rice mildew, rice plague, rice strain rot, Rice Blight, Rice Seed Bacterial Disease, Rice Blast Bacterial Disease, Rice Blight, Rice Brown Mycobacterium, Rice Brown Small Mycobacterium, Rice Brown Herring Blight, Rice Saccharomyces, Rice Black Grain Nuclear disease, rice black sclerotia nuclear disease, rice staphylococcal nuclear disease, rice white silk disease, rice red fungal disease, rice wilt disease, rice seed rot, rice seed rot, rice gray sclerotial disease, rice fool Seedling disease, rice leaf sheath net blotch, rice wilt disease, cotton wilt disease, broad bean wilt disease, broad bean plague, broad bean nuclear disease, broad bean stem rot, broad bean black root disease, broad bean white silk disease, broad bean white coat disease , Broad bean wilt disease, broad bean root rot, soybean leaf spotted virus disease, soybean leaf spot disease, soybean leaf burning disease, da Izu spot bacterial disease, soybean wilt disease, soybean strain blight, soybean sclerotia, soybean stem blight, soybean black root disease, soybean black root rot, soybean white silk disease, soybean blight, soybean rhizoctonia root rot, okra disease , Okra blight, okra half wilt, spinach mosaic disease, spinach wilt, spinach plague, spinach strain rot, spinach mold, spinach wilt, spinach verticillium wilt, spinach root rot, rose Root head cancer, Rose blight, Rose half-wilt, Hollyhock white silk disease, Bubaldia seedling blight, Godetia blight, Stock black rot, Stock wilt, Stock blight, Stock strain disease, Stock blight Disease, stock seed rot, stock seedling blight, stock half body wilt, ha button yellow ill, acidansera heart rot, aciddanse Soft rot, Acidan sella white rot, Acidan seraphrotis rhizome rot, Acidan sera dry rot, Acidan sella strain, Acidan serra white rot, Crocus soft rot, Crocus dry rot, Crocus sclerosis, Crocus stalk rot, Crocus Mycorrhizal disease, Hinogi silkworm, Freesia neck rot, Freesia bulb rot, Freesia mycosis, Freesia blight, Alstroemeria plague, Alstroemeria mycosis, Alstroemeria scab, Almeria scab, Staticis wilt Disease, static disease, static disease, static disease, static disease, static white disease, gloxinia disease, saintpaulia disease, whitebait white silk disease, ominae half-wilt disease, soft cherry rot, canna stem rot, campanula fungus, Campanula white silk disease, Campanula root rot, Kiki Corn blight, aster wilt, aster blight, gerbera plague, gerbera nuclear disease, gerbera white silk disease, gerbera root rot, gerbera half body wilt, gerbera spotted bacterial disease, gazania sclerotia, chrysanthemum wilt Disease, chrysanthemum root rot, chrysanthemum rot, chrysanthemum wilt, chrysanthemum plague, chrysanthemum fungal disease, chrysanthemum silkworm, chrysanthemum wilt, chrysanthemum wilt, chrysanthemum stem blight, chrysanthemum white feather Disease, calendula plague, calendula sclerosis, calendula half wilt, cosmos wilt, cosmos half wilt, zion white wilt, zinnia bacterial wilt, zinnia soft rot, zinnia spot bacterial disease, zinnia sclerotia, zinnia Blight, Cinaria seedling blight, Shasta daisy half body wilt, Shukukon Aster white silk disease, Shukukon Aster spot disease, Solida star white silk disease, Dahlia blight, Dahlia root cancer, Dahlia soft rot bacteria Disease, dahlia Nuclear disease, dahlia silkworm, dahlia seedling blight, daisy blight, harshagik blight, sunflower blight, sunflower leaf blight, sunflower spot bacterial disease, sunflower bacilli, sunflower white silk, Sunflower seedling blight, Pirogek fungus nuclear disease, Fujibaka white silk disease, safflower soft rot, safflower rot disease, Margaret blight, Margaret root cancer disease, Margaret wilt disease, Margaret sclerotia, Marigold blue Blight, Marigold strain, Miyakowase root cancer, Miyakowa wilt, Miyakowa plague, Miyakowa rot, Miyakowa white silkworm, Wheat husk wilt disease, cornflower, cornflower, riatris Mycorrhizal disease, Riatris white silk disease, Rudbeckia wilt disease, Rudbeckia white silk wilt, Rudbeckia half body wilt, Aferrandra plague, D Periwinkle, periwinkle plague, periwinkle nuclear disease, seedling blight of periwinkle, anemone plague, anemone bulb rot, anemone tuberculosis, columbine white silkworm, clematis root cancer, clematis white silkworm, peonies Root Cancer, Peonies Blight, Peonies Bacterial Diseases, Peonies White Silk Blossoms, Peonies White Blight, Prunus White Silk Blossoms, Gypsophila White Silk Blossoms, Delphinium Soft Blight, Delphinium White Silk Blossoms, Delphinium Blight, Hana Aconite half-wilt, spotted beetle spot disease, Fusarium root rot fungus nuclear disease, ranunculus spotted bacterial disease, poppy wilt disease, poppy soft rot, poppy spot spot bacterial disease, poppy fungus nuclear disease, poppy white crest feather Disease, poppy head blight, poppy seedling blight, wilt root rot, peperomia white silk, peperomia rot, calceorari Seedling blight, Snapdragon spot bacterial disease, Snapdragon plague, Snapdragon sclerosis, Snapdragon stem rot, Snapdragon sclerotia, Snapdragon root disease, Snapdragon root rot, Snapdragon half wilt, Primula soft rot, Primula Leaf Bacterial Disease, Primula Rot, Primula Seedling, Primrose Spot, Cyclamen Soft Rot, Cyclamen Dwarf, Cyclamen Seed Rot, Cyclamen Seed Rot, Color Soft Rot, Color Epidemic, Color White Silk, Caldium Mycorrhizal disease, Caradium white silkworm, Philodendon plague, Cactus blight, Cactus stem blight, Cactus stem rot, Cactus rot, Ajuga white silk, Kaktranoo white silk, Salvia blight, Begonia spotted bacterial disease, Begonia stem rot Disease, water lily spotted rot, portulaca blight, violet root rot, white lacef Lower wilt disease, white lace flower plague, white lace flower blight, murasaki omoto blight, spinach rot, spinach plague, spinach white silkworm, spinach blight, petunia mold, petunia mildew, spotted physalis Disease, physalis white silkworm, physalis half body wilt disease, carnation wilt bacterial disease, carnation wilt bacterial disease, carnation spotted bacterial disease, carnation wilt disease, carnation plague, carnation sclerotia, carnation stem rot, carnation neck rot, Carnation white silk disease, carnation blight, carnation root rot, gypsophila disease, gypsophila nuclear disease, gypsophila wilt bacterial disease, gypsophila knot disease, gypsophila root cancer, gypsophila spot Bacteria , Gypsophila spp., Gypsophila stem rot, Gypsophila stem blight, Gypsophila seedling rot, Strelitzia bacterial wilt, Strelitzia pestis, Amaryllis plague, Ukezakikun silane silkworm, Narcissus soft rot, Narcissus Dry rot, narcissus bulb rot, narcissus rot, narcissus white rot, neline plague, neline white silkworm, limpet white silkworm, celosia blight, celosia root rot, gypsum root rot, geranium stem rot Disease, geranium blight, geranium spot disease, Kalanchoe plague, sweet pea wilt, sweet pea sclerosis, sweet pea half wilt, lupine wilt, lupine sclerotia, lupine white silkworm, lupine white coat, lupine standing Blight, Allium plague, Aloe plague, Ornithogalum plague, Omoto white rot, Hosta white silk , Colchicum root rot, Thundersonia white silk disease, Sandersonia wilt disease, Janohige white silk disease, Tulip black rot, Tulip soft rot, Tulip blue mold, Tulip plague, Tulip bulb rot, Tulip bulb rot, Tulip Mycorrhizal disease, Tulip stem blight, Tulip silkworm, Tulip white rot, Tulip gray rot, Tulip root rot, Tulip rot, Turbakia white rot, Dracaena plague, Triteria plague, New Sailan silkworm, Haran fungus Nuclear disease, hyacinth soft rot, hyacinth rot, hyacinth fungus nuclear disease, hyacinth white silk disease, hyacinth white plague, photogis white silk disease, lily soft rot, lily blue mold, lilies plague, lily black rot fungus Disease, lily white silkworm, lily purple herringbone disease, lucas spotted disease, tsutsumori Bacterial plague, brown rot, Onsidium soft rot, Oncidium plague, Cattleya rot, Cattleya brown bacterial disease, Cattleya plague, Cattleya soft rot, Cattleya seed black rot, Cymbidium brown rot, Cymbidium soft rot, Cymbidium plague, cymbidium brown leaf blight, cymbidium white silk disease, cymbidium seed silk rot, cymbidium rot disease, dendrobium brown rot disease, dendrobium soft rot, dendrobium white silk disease, dendrobium seed rot, dendrobium rot, paphiopedilum brown Rot, paphiopedilum soft rot, banda brown rot, banda soft rot, banda plague, banda white silk disease, bilstechela brown rot, farenopsis brown rot, farenopsis brown bacterial disease, farenopsis soft rot, farenopsis white silk disease, fa Knops strain blight, Miltonia brown rot, Exacam strain blight, Eustoma plant blight, Eustoma strain rot, Eustoma spp.nucleus, Eustoma stem rot, Eustoma root rot, Eustoma root rot, Gentian leaf rot, Gentian Brown root rot, gentian white silk disease, gentian leaf rot, gentian flower rot fungus, gentian knot, poinsettia root rot, mango root cancer, oyster head cancer, oyster white coat Feather disease, oyster purple herb disease, oyster homoposis withering disease, gummy white crest feather disease, gummy micronucleus nuclear disease, fig root cancer disease, fig epidemic, fig fungus nuclear disease, fig white silk disease, fig white Herb disease, fig soft rot, fig purple herb disease, passion fruit plague, passion fruit mycosis, pineapple flower rot, pineapple heart rot, papaya root cancer Papaya, papaya seedling blight, papaya soft rot, apricot root rot, apricot bald rot, apricot purple crest, ume root rot, ume blight, ume mycosis, ume White rot, ume purple crest, sugar beet root cancer, sweet potato nuclear disease, white blossom rot, sweet purple scab, karin bald rot, raspberry root rot, Plum root rot, Prunus white rot, Prunus purpura, pear plague, pear bald rot, pear root rot, pear pest, pear nuclear disease, pear white crest Feather disease, pear purple crest, loquat root cancer, loquat plague, loquat white coat, loquat purple crest, quince root cancer, peach root cancer, peach fungus Nuclear disease, peach bald scab, peach purple scab, apple root rot, apple blight, apple bald, apple bald, apple violet Grape Root Cancer, Grape White Coat, Grape Half Body Dwarf, Grape Purple Coat, Chestnut Root Cancer, Chestnut Epidemic, Chestnut White Coat, Chestnut Purple Coat, Kiwi Fruit Examples include white coat feather disease, citrus root cancer disease, citrus nuclear disease, citrus white coat feather disease, citrus purple coat disease, and citrus Fusarium wilt disease.

  On the other hand, the present invention secondly provides an assay method for finding microorganisms useful for controlling soil infectious diseases accompanied by the promotion of crop growth. That is, in this method for assaying useful microorganisms, the test microorganism is applied to the culture soil or crop seed, the soil pH correction substance is applied to the culture soil, the plant disease resistance inducer is applied to the culture soil or crop foliage, When soil-inhabiting phytopathogenic fungi are cultivated by adding them to soil or crop foliage, and as a result, the microorganisms under test tend to increase the disease-inhibiting effect when combined with soil pH-correcting substances and plant disease resistance-inducing substances Is determined to be a useful microorganism for controlling soil-borne diseases that accompany the growth of crops. In addition, the test microorganisms are applied to the soil or crop seed, the soil pH correction substance is applied to the culture soil, and then the plant disease resistance inducer is applied to the soil or crop foliage and cultivated. Is a microorganism that is useful for controlling soil-borne diseases that involve the growth of crops, even when the growth of crops tends to be promoted by combining with a soil pH corrector and a plant disease resistance inducer. judge.

  The soil pH-correcting substance, plant disease resistance-inducing substance, and target crop used in the test method can be selected from those described in the above-mentioned soil infectious disease control method that accompanies the growth of crops. In addition, it is preferable to give the soil of a farm field to the cultivation soil so that it may approach a farm field environment. In this case, it is preferable to apply 1 to 10 parts by mass of soil in the field with respect to 100 parts by mass of the soil. The soil in the field may be applied after being suspended in water or the like.

Examples of soil-habiting plant pathogens include melon vine split fungus, melon necrotic spot disease fungus, melon mosaic virus, melon brown spot bacterial disease fungus, melon oncogenic fungus, melon soft rot fungus, melon spot fungus disease fungus, melon hair root disease fungus , Melon causal fungus, melon sclerotia fungus, melon red root rot fungus, melon black spot root rot fungus, melon white silkworm fungus, melon blight fungus, melon vine blight fungus, melon root rot fungus, melon root rot fungus, melon root Rot wilt fungus, melon half body wilt fungus, pumpkin wilt fungus, pumpkin brown bacterial fungus, pumpkin spot bacterial fungus, pumpkin plague fungus, pumpkin white silk fungus, pumpkin wilt fungus, pumpkin vine blight fungus, cucumber green spot mosaic virus, Cucumber bacterial wilt fungus, cucumber brown spot fungus, cucumber soft rot fungus, cucumber spotted bacterial fungus, cucumber plague fungus, cucumber brown spot fungus, Cucumber nuclei, cucumber white silk, cucumber vine blight, cucumber vine split, cucumber seedling blight, cucumber root rot, cucumber gray plague, cucumber half wilt, cucumber homoposis root rot, cucumber purple crest Feather fungus, watermelon green spot mosaic virus, watermelon wilt bacterial fungus, watermelon brown spot bacterial fungus, watermelon epidemic fungus, watermelon mycorrhizal fungus, watermelon black spot root fungus, watermelon white silkworm fungus, watermelon stand blight fungus, watermelon vine blight fungus, Watermelon vine split fungus, watermelon half body wilt fungus, watermelon Fusarium blight fungus, pickaxe spotted bacterial fungus, Tougan blight fungus, Togan vine blight fungus, Togan vine split fungus, loofah vine split fungus, loofah rot fungus, loofah seedling Bacterial fungus, Yugao brown spot bacterial fungus, Yugao spotted bacterial fungus, Yugao black spot rot fungus, Yugao white silk fungus, Yugaotsu Bacterial fungus, Pepper vulgaris, Pepper seedling blight fungus, Pepper gray blight fungus, Pepper green pepper blight fungus, Pepper pepper sweet potato fungus, Pepper pepper sweet pepper, Pepper pepper sweet spot, Pepper wilt , Pepper Pepper Bacteria, Pepper Pepper Nuclei, Pepper Pepper Black Spot Rot, Pepper Pepper White Silk Pepper, Pepper Pepper Standing Bacteria, Pepper Pepper Seedling Bacterium, Pepper Pepper Half Body Dwarf , Tomato streak fungus, tomato mosaic virus, tomato bacterial wilt fungus, tomato scab fungus, tomato stem rot fungus, tomato black blight fungus, tomato soft blight fungus, tomato spot fungus fungus, tomato spotted fungus fungus, tomato spoilage fungus , Tomato alternaria stem blight fungus Tomato wilt fungus, tomato blight fungus, tomato brown root rot fungus, tomato brown rot fungus, tomato fungus rot fungus, tomato red root rot fungus, tomato black root rot fungus, tomato granule fungus fungus, tomato white silk fungus, tomato seedling Blight fungus, tomato root rot fungus, tomato root rot wilt fungus, tomato root rot fungus, tomato gray wilt fungus, tomato half-wilt fungus, eggplant mosaic virus, eggplant bacterial wilt fungus, eggplant brown spot fungus, eggplant stem fungus Bacterial fungus, eggplant stem rot fungus, eggplant soft rot fungus, eggplant spotted fungal fungus, eggplant plague fungus, eggplant brown rot fungus, eggplant mycorrhizal fungus, eggplant black spot root fungus, eggplant white silkworm fungus, eggplant seedling blight fungus, eggplant root Rot fungus, eggplant wilt fungus, eggplant half wilt fungus, cauliflower black rot fungus, cauliflower black spot bacterium, cauliflower soft rot fungus, cauliflower wilt fungus, cauliflower root knot fungus, cabbage black rot fungus Cabbage black spot bacterial fungus, cabbage soft rot fungus, cabbage wilt fungus, cabbage strain rot fungus, cabbage rot fungus, cabbage root rot fungus, cabbage root knot fungus, cabbage verticillium wilt fungus, cabbage seedling blight fungus, komatsuna wilt fungus , Spotted rhinoceros, fungus black rot fungus, Chinese cabbage black spot bacterial fungus, Chinese cabbage soft rot fungus, Chinese cabbage rot fungus, Chinese cabbage yellow rot fungus, Chinese cabbage fungus nuclear fungus, Chinese cabbage rot fungus, Chinese cabbage root constriction fungus, Chinese cabbage Root-knot fungus, cabbage spoilage fungus, broccoli picium spoilage fungus, turnip blight fungus, turnip black rot fungus, turnip black blight fungus, turnip soft rot fungus, turnip wilt fungus, turnip root rot fungus, turnip root rot fungus, turnip root Spoilage fungus, turnip root-knot fungus, turnip root-knot fungus, turnip fungus Japanese radish blight fungus, Japanese radish black rot fungus, Japanese radish black spot rot fungus, Japanese radish black spot bacterial fungus, Japanese radish scab fungus, Japanese radish soft rot fungus, Japanese radish yellow rot fungus, Japanese radish round brown rot fungus, Japanese radish rot fungus, Japanese radish black spot disease fungus , Japanese radish root rot fungus, Japanese radish root rot fungus, Japanese radish leaf rot fungus, Japanese radish vertebrate black spot fungus, Japanese radish rot fungus, Japanese radish rot fungus, Wasabi sclerotia fungus, Wasabi stem rot fungus, Wasabi rooted fungus, Wasabi root rot fungus Bacterial fungus, powdery mildew fungus, powdery mildew fungus, powdery mildew fungus, nuclear fungus of powdery mildew, white silkworm fungus, powdery mildew fungus, shungy wilt fungus, shungyiku black rot fungus, shungyiku rot fungus, shungyak wilt fungus Disease fungus, lettuce soft rot fungus, lettuce spotted bacterial fungus, lettuce spoilage fungus, lettuce rot fungus, lettuce granule nuclei fungus, lettuce Bacterial fungus, lettuce root rot fungus, celery soft rot fungus, celery leaf bacterial fungus, celery leaf blight fungus, celery rot fungus, celery wilt fungus, celery mycobacterium, parsley soft rot fungus, parsley wilt fungus, parsley epidemic fungus, parsley Blight fungus, Parsley seedling blight fungus, Parsley root rot fungus, Parsley root rot fungus, Strawberry horn spot bacterial fungus, Strawberry wilt fungus, Strawberry wilt fungus, Strawberry plague fungus, Strawberry fruit spoilage fungus, Strawberry rot fungus, Strawberry black Root rot fungus, Strawberry white silk fungus, Strawberry soft rot fungus, Strawberry root rot fungus, Strawberry bud blight fungus, Asparagus brown fungus Root root rot fungus, Asparagus strain fungus, Asparagus white coat fungus, Asparagus blight fungus Asparagus seedling blight fungus, asparagus purple crest fungus, onion scab fungus, onion soft rot fungus, onion spotted bacterial fungus, Onion rot fungus, onion fragment rot fungus, onion plague fungus, onion dry rot fungus, onion sclerotia fungus, onion black mold fungus, onion black rot fungus fungus, onion smut fungus, onion red root rot fungus, onion small fungus scleroderma fungus , Onion white silk fungus, onion white plague fungus, onion seedling blight fungus, leek strain rot fungus, leek soft rot fungus, leek dry rot fungus, leek black rot rot fungus, leek red root rot fungus, leek white silk rot fungus, Leek white blight fungus, leek leaf rot fungus, leek leaf rot fungus, leek soft rot fungus, leek spot bacterial fungus, leek rot fungus, leek wilt disease fungus, leek blight fungus, leek black rot fungus, fungi, leek black rot fungus, leek red root fungus Bacterial fungi, leek small sclerotia, leek white silkworm, leek white blight fungus, leek seedling blight fungus, sweet potato blight fungus, sweet potato blue mold fungus, sweet potato scab fungus, sweet potato brown dry rot fungus Potato sclerotia, sweet potato black rot fungus, sweet potato black spot fungus, sweet potato granule fungus, sweet potato white silk fungus, sweet potato white rot fungus, sweet potato white coat fungus, sweet potato charcoal fungus, sweet potato vine split fungus, sweet potato soft rot fungus , Sweet potato root rot fungus, sweet potato gray mold fungus, sweet potato purple crest fungus, ginger rot fungus, ginger rhizome rot fungus, ginger stand blight fungus, ginger blight fungus, carrot knot fungus, carrot root carcinogen, carrot Streptomyces scab, carrot soft rot, carrot spot bacterial, carrot dwarf, carrot brown root rot, carrot dry rot, carrot sclerotia, carrot black root rot, carrot black root rot, carrot stain rot Carrot white silkworm, carrot scab, carrot root rot fungus, carrot Gin purple herb fungus, rice rice mildew fungus, rice blight fungus, rice strain blight fungus, rice white leaf blight fungus, rice seedling bacterial fungus, rice maple blight fungus, rice blight fungus, rice brown fungus nuclei fungus, rice brown Bacterial rot fungus, rice brown blight fungus, rice saccharomyces rot fungus, rice black rot fungus fungus, rice small black rot fungus fungus, rice staphylococcal blast fungus, rice white silk rot fungus, rice red fungus rot fungus, rice Bacterial fungus, rice seed rot fungus, rice seedling blight fungus, rice gray fungus rot fungus, rice stupid seedling fungus, rice leaf sheath fungus, rice blight fungus, rice cotton wilt fungus, broad bean wilt fungus, broad bean fungus , Broad bean nuclear fungus, broad bean stem rot fungus, broad bean black root fungus, broad bean white silk fungus, broad bean white rot fungus, broad bean wilt fungus, broad bean root rot fungus, soybean leaf spotted virus, soy bean leaf fungus, soybean leaf burning Pathogen, soybean spot bacterial pathogen, soybean wilt , Soybean strain blight fungus, soybean sclerotia fungus, soybean stem blight fungus, soybean black root fungus, soybean black root rot fungus, soybean white silk fungus, soybean blight fungus, soybean rhizobonia root rot fungus, okra blight fungus, okra blight fungus, Okra half wilt fungus, spinach mosaic virus, spinach wilt fungus, spinach wilt fungus, spinach strain rot fungus, spinach mold fungus, spinach wilt fungus, spinach wilt disease fungus, spinach root rot fungus, rose root cancer Disease fungus, rose blight fungus, rose half body wilt fungus, holyhock white silkworm fungus, bouvardia seedling blight fungus, godetia blight fungus, stock black rot fungus, stock wilt fungus, stock blight fungus, stock strain blight fungus, stock Seedling fungus, stock seedling blight fungus, stock half body wilt fungus, c Tan dwarf fungus, acidan serra heart rot fungus, acidan serra soft rot fungus, acidan sera white silk septic fungus, acidan sera rotoris rhizome rot fungus, acidan sella dry rot fungus, acidan sella fungus, acidan serra white silk sage fungus, crocus soft rot fungus, crocus dry rot fungus, crocus Coccus sclerosis fungus, Crocus coccus rot fungus, Crocus coccus rot fungus, Heigi white silk fungus, Freesia neck rot fungus, Freesia bulb rot fungus, Freesia sclerotia fungus, Freesia wilt fungus, Alstroemeria spp. Silk fungus, Almeria white silk fungus, Statice wilt fungus, Statice wilt bacterial fungus, Stachys fungus, Stachys strain rot fungus, Stachys white silk fungus, Gloxinia fungus, St.paulia fungus, Iwahiba white silk fungus, Ominaeshi half body wilt Gonorrhea, soft cherry rot, canna stem rot, campanula rot fungus, campanula white silk fungus, campanula root rot fungus, eucalyptus fungus, aster wilt fungus, aster wilt fungus, gerbera epidemic fungus, gerbera rot fungus, Gerbera white silk fungus, Gerbera root rot fungus, Gerbera half body wilt fungus, Gerbera spotted bacterial fungus, Gazania sclerotia fungus, chrysanthemum wilt fungus, chrysanthemum root rot fungus, chrysanthemum rot fungus, chrysanthemum wilt fungus, chrysanthemum plague fungus, Chrysanthemum fungus, chrysanthemum silk fungus, chrysanthemum blight fungus, chrysanthemum wilt fungus, chrysanthemum stem blight fungus, chrysanthemum white fungus fungus, calendula bacteriomycosis, calendula fungus sclerotic fungus, calendula half body wilt fungus, cosmos blight fungus, Cosmos half wilt fungus, Zion silkworm fungus, Zinnia bacterial wilt fungus, Zinnia soft rot fungus, Zinnia spotted bacterial fungus, Zinnia sclerotia fungus, Zinnia bacterial wilt fungus Laria seedling blight fungus, shasta daisy half body wilt fungus, shukukonaster white silk fungus, shukukonaster spotted fungus, soridaster white silkworm fungus, dahlia bacterial blight fungus, dahlia root rot fungus, dahlia soft bacteriomycosis fungus, dahlia fungus Nuclear fungus, dahlia silkworm fungus, dahlia seedling blight fungus, daisy fungus blight fungus, harshagik blight fungus, sunflower blight fungus, sunflower leaf blight fungus, sunflower spotted fungal fungus, sunflower fungus nuclear fungus, sunflower white silk fungus, Sunflower seedling blight fungus, Pyrethrum nuclei fungus, Fujibacama white silk fungus, safflower soft rot fungus, safflower rot fungus, Margaret wilt fungus, Margaret root cancer fungus, Margaret wilt fungus, Margaret fungus nuclei, Marigold blue Blight fungus, Marigold strain rot fungus, Miyakowase root cancer fungus, Miyakowase dwarf , Miyakowasae plague fungus, Miyakowas strain rot fungus, Miyakowasle white silk fungus, wheat wagtail half body wilt fungus, corn rot fungus, cornflower white rot fungus, riatris rot fungus, riatris white silk fungus, rudbeckia wilt fungus, rudbeckia wilt Half body wilt fungus, Aferrandra plague fungus, Catharanthus rosea yellow fungus, Catharanthus roseus fungus, Catharanthus periwinkle nuclear fungus, Catharanthus periwinkle, Anemone plague fungus, Anemone bulb rot fungus, Anemone white fungus Fungus, clematis white silk fungus, peony root cancer fungus, peonies blight fungus, peony nuclei fungus, peonies white silk fungus, peony stem blight fungus, prunus white silk fungus, scaboon white silk fungus, delphinium soft rot fungus, Delphinium silkworm Fungus, Delphinium blight fungus, Hanabikobu half body wilt fungus, Hanaburi kabu spot blight fungus, Fuchusou root rot fungus, Bacteria ranunculus spot bacterial disease, Poppy dwarf yellow fungus, Poppy softened spoilage fungus, Poppy spot bacteriomycosis fungus, Poppy fungus Nuclear fungus, poppy white crested fungus, poppy seed blight fungus, poppy seed blight fungus, wilt root rot fungus, peperomia white silk fungus, peperomia spoilage fungus, calceolaria seedling blight fungus, snapdragon spotted fungus fungus, snapdragon Fungus, snapdragon rot fungus, snapdragon stem rot fungus, snapdragon granule fungus, snapdragon white silkworm fungus, snapdragon root rot fungus, snapdragon half body wilt fungus, primrose soft rot fungus, primrose rot fungus, primrose rot Bacterial fungus, primrose fungus, cyclamen soft rot fungus, cyclamen Gonorrhea, Cyclamen seedling fungus, Cyclamen seedling blight fungus, Color soft rot fungus, Color blight fungus, Color white silkworm fungus, Caradium rot fungus, Caradium white silkworm fungus, Philodendon fungus, cactus blight fungus, cactus stem blight fungus, Cactus stem rot fungus, cactus spoilage fungus, Ajuga white silk fungus, Kaktranoo white silk fungus, Salvia epidemic fungus, Begonia spot bacterial fungus, Begonia stem rot fungus, water lily spotted spoilage fungus, Porchula rot fungus, violet root rot fungus, white Lace flower wilt fungus, white lace flower blight fungus, white lace flower blight fungus, murasakiomoto blight fungus, spinach rot fungus, spinach blight fungus, spinach white silk fungus, spinach blight fungus, petunia rot fungus, petunia mildew Physalis spotted bacterial fungus, physalis white silkworm, Viburnum wilt fungus, carnation wilt bacterial fungus, carnation blight fungus fungus, carnation spotted fungus fungus, carnation wilt fungus, carnation fungus fungus, carnation fungus karyophilic fungus, carnation stem rot fungus, carnation head rot fungus, carnation white silk fungus, carnation Bacterial fungus, carnation root rot fungus, gypsophila plague fungus, gypsophila nuclei fungus, gypsophila wilt bacterial fungus, gypsophila knot fungus, gypsophila root fungus fungus, gypsophila spot blotch Gypsophila fungus, Gypsophila stalk rot fungus, Gypsophila spp. Bacterial fungus, Gypsophila seedling rot fungus, Strelitzia bacterial wilt fungus, Strelitzia pesticidal fungus, Amaryllis pest fungus, Ukezakikun silane white silk Disease fungus, narcissus soft rot fungus, narcissus dry rot fungus, narcissus bulb rot fungus, narcissus rot fungus, daffodil white coat fungus, neline plague fungus, nerine white silk fungus, hamaomoto white silk fungus, celosia plague fungus, celosia blight fungus , Cereal root rot fungus, geranium stem rot fungus, geranium stem blight fungus, geranium leaf blight fungus, Kalanchoe epidemic fungus, sweet pea wilt fungus, sweet pea rot fungus, sweet pea half wilt fungus, lupine wilt fungus, lupine fungus sclerosis fungus, lupine white silk Bacterial fungus, lupine white crest fungus, lupine blight fungus, allium blight fungus, aloe bacteriomycosis, ornithogalum fungus, omoto white crest fungus, oryzyl orchid white silk fungus, gypsophila white silk fungus, corticum root rot fungus, sandersonia White silkworm fungus, sandersonia blight fungus, janoge white silkworm fungus, tulip black rot , Tulip soft rot fungus, tulip blue mold fungus, tulip blight fungus, tulip bulb rot fungus, tulip bulb rot fungus, tulip fungus nucleate fungus, tulip stem blight fungus, tulip white silk fungus, tulip white blight fungus, tulip gray rot fungus, tulip root Rot fungus, Tulip rot fungus, Thurbachian white silkworm, Dracaena plague fungus, Triteria plague fungus, New syran white silkworm fungus, Haran sclerotia fungus, hyacinth soft rot fungus, hyacinth spoilage fungus, hyacinth rot fungus, hyacinth scabinous fungus, hyacinth white Blight fungus, white rot fungus, lily soft rot fungus, lily blue mold fungus, lily pesticidal fungus, lily black rot fungus nuclear fungus, lily white silkworm fungus, lily purple crest fungus, ruscus spotted fungus, japonica Phytophthora, pertussis brown rot , Oncidium soft rot fungus, Oncidium pesticidal fungus, Cattleya brown rot fungus, Cattleya brown rot fungus, Cattleya spoilage fungus, Cattleya soft rot fungus, Cattleya satan black rot fungus, Cymbidium brown rot fungus, Cymbidium soft rot fungus, Cymbidium fungus fungus, Cymbidium brown leaf Bacterial fungus, cymbidium white silkworm, cymbidium seedling black rot fungus, cymbidium spoilage fungus, dendrobium brown rot fungus, dendrobium soft rot fungus, dendrobium white silkworm fungus, dendrobium black rot fungus, dendrobium rot fungus, paphiopedilum brown rot fungus, paphiopedilum soft rot fungus , Banda brown rot fungus, Banda soft rot fungus, Banda plague fungus, Banda white silk fungus, Bilstekera brown rot fungus, Phalaenopsis brown rot fungus, Phalaenopsis brown spot bacterial fungus, Phalaenopsis soft rot fungus, Phea Lenopsis white silkworm, Phalaenopsis strain blight fungus, Miltonia brown rot fungus, Exacam strain blight fungus, Eustoma spp. Gentian leafy fungus, Gentian brown root rot fungus, Gentian white silkworm, Gentian leaf rot fungus, Gentian flower rot fungus nucleophilic fungus, Gentian rot-causing virus, Poinsettia root rot fungus, mango root rot fungus, oyster root Cancer fungus, oyster white crest fungus, oyster purple crest fungus, oyster homoposis blight fungus, gummy white crest fungus, gummy microtuberculosis fungus, fig root fungus fungus, fig blight fungus, fig fig nucleus Pathogen, fig white silk fungus, fig white coat fungus, fig soft rot fungus, fig purple coat fungus, passion Root plague fungus, passion fruit sclerotia, pineapple flower rot fungus, pineapple heart rot fungus, papaya root cancer fungus, papaya seedling blight fungus, papaya soft rot fungus, apricot root rot fungus, apricot white crest feather Bacterial fungus, apricot purple crest fungus, ume root carcinogenic fungus, ume blight fungus, ume fungus nuclear fungus, ume white crested fungus fungus, ume purple crest feather fungus, sweet potato root carcinogenic fungus, sweet potato sclerotic fungus, Sweet cherry white rot fungus, sweet cherry purple rot fungus, Karin white coat fungus, raspberry root fungus fungus, plum root fungus fungus, plum white bald fungus, plum purple fungus, pear plague fungus , Pear white crest fungus, pear root fungus fungus, pear blight fungus, pear fungus nuclear fungus, pear white crest fungus, pear purple crest fungus, loquat root cancer fungus, loquat plague fungus, loquat White crested fungus, Biwa purple crest , Quince root rot fungus, peach root crest fungus, peach fungus nuclei fungus, peach bald fungus, peach purple crest fungus, apple root crest fungus, apple blight fungus, apple white silk fungus , Apple white crest fungus, apple purple crest fungus, grape head crest fungus, grape white crest fungus, grape half body wilt fungus, grape purple crest fungus, chestnut root crest fungus, chestnut blight fungus, Chestnut white coat fungus, chestnut purple coat fungus, kiwifruit white coat fungus, citrus root crest fungus, citrus nuclei fungus, citrus white coat fungus, citrus purple coat fungus, citrus Fusarium wilt fungus, etc. Is mentioned.

  Application of test microorganisms, soil pH correction substances, field soil, plant disease resistance inducers, and / or soil-habiting phytopathogenic fungi to sowing soil or culture soil, mixing with soil, irrigation treatment, spraying, etc. Can be performed. Application of test microorganisms, plant disease resistance inducers, and / or soil-habiting phytopathogenic fungi to crops, crop seeds, or crop foliage should be carried out by spraying, applying, dipping, or injecting the crops, etc. Can do.

  In the method for assaying useful microorganisms of the present invention, from the viewpoint of workability for handling a large number of candidate microorganisms, the culture medium is put in a cell tray cell having about 32 to 200 cells having a capacity of 10 to 300 ml. Depending on the target crop without seeding and then transplanting the plant afterwards, depending on the target crop, for example, about 1 to 6 weeks after sowing, the production weight of the plant body above the border or cotyledon is measured. Thus, it is preferable to determine whether or not it is useful for controlling soil infectious diseases accompanied by the promotion of crop growth based on the value. The criteria for determining whether or not it is useful can be determined as appropriate.

  On the other hand, the present invention also provides, thirdly, an assay method for finding a soil pH correcting substance useful for controlling soil infectious diseases accompanied by the promotion of crop growth. That is, in this soil pH correction substance test method, useful microorganisms are added to the soil or crop seed, acidic or alkaline test substances are applied to the culture soil, and plant disease resistance inducers are applied to the soil or crop foliage. Then, soil-inhabiting phytopathogenic fungi are applied to the soil or crop foliage and cultivated, and as a result, the tested acid or alkaline substances combine with useful microorganisms and plant disease resistance inducers to prevent disease. In the case of showing a tendency to increase, it is determined that the substance is a soil pH correcting substance useful for a soil infectious disease control method that accompanies the growth of crops. Also, useful microorganisms are applied to the soil or crop seeds, acidic or alkaline test substances are applied to the culture soil, and then plant disease resistance inducers are applied to the culture soil or crop foliage for cultivation. Soil pH useful for soil infectious disease control methods with crop growth promotion even when the growth of crops tends to be promoted by the combined use of acidic or alkaline substances with useful microorganisms and plant disease resistance inducers Judged as a corrective substance.

  Useful microorganisms, plant disease resistance inducers, and target crops to be used in the assay method can be selected from those described in the above-mentioned soil infectious disease control method that accompanies the growth of crops. In addition, it is preferable to give the soil of a farm field to the cultivation soil so that it may approach a farm field environment. In this case, it is preferable to apply 1 to 10 parts by mass of soil in the field with respect to 100 parts by mass of the soil. The soil in the field may be applied after being suspended in water or the like.

  As the soil-habiting phytopathogenic fungi, those similar to the above can be used.

  Application of useful microorganisms, acidic or alkaline substances to be tested, field soil, plant disease resistance inducers, and / or soil-inhabiting phytopathogenic fungi to sowing or cultivating soil, mixing with soil, irrigation treatment, This can be done by spraying. Application of useful microorganisms, plant disease resistance inducers, and / or soil-habiting phytopathogenic fungi to crops, crop seeds, or crop foliage may be carried out by spraying, applying, dipping, or injecting the crops, etc. it can.

  In the soil pH correcting substance testing method of the present invention, from the viewpoint of workability for handling a large number of candidate acidic substances or candidate alkaline substances, the inside of a cell tray having about 32 to 200 cells having a capacity of 10 to 300 ml. Put the soil into the seeds, sow seeds in this, and then transplant the plants without thinning them, and thin them out as appropriate. Depending on the target crop, for example, about 1 to 6 weeks after sowing, the plant above the border or cotyledon It is preferable to measure the production weight of the body and determine whether or not it is useful for controlling soil infectious diseases accompanied by the promotion of crop growth based on the value. The criteria for determining whether or not it is useful can be determined as appropriate.

  On the other hand, the present invention fourthly provides an assay method for finding a plant disease resistance inducer useful for controlling soil infectious diseases accompanied by the promotion of crop growth. That is, in this assay method for plant disease resistance inducers, useful microorganisms are added to the soil or crop seeds, soil pH correction materials are applied to the soil, test substances are applied to the soil or crop foliage, and then the soil Cultivate crops in which habitat phytopathogenic fungi are cultivated by adding them to culture soil or crop foliage, and as a result, the tested substances tend to increase the disease control effect when combined with useful microorganisms and soil pH correcting substances. It is judged that it is a plant disease resistance inducer useful for soil infectious disease control method accompanied by growth promotion. Further, useful microorganisms are applied to the soil or crop seeds, soil pH-correcting substances are applied to the culture soil, and then the test substances are applied to the culture soil or crop foliage and cultivated. Even in the case where the growth of crops tends to be promoted by combining with a soil pH correcting substance, it is determined that the substance is a plant disease resistance inducer useful for a soil infectious disease control method involving the growth of crops.

  Useful microorganisms, soil pH-correcting substances, and target crops used in the assay method can be selected from those described in the above-mentioned soil infectious disease control method that accompanies the growth of crops. In addition, it is preferable to give the soil of a farm field to the cultivation soil so that it may approach a farm field environment. In this case, it is preferable to apply 1 to 10 parts by mass of soil in the field with respect to 100 parts by mass of the soil. The soil in the field may be applied after being suspended in water or the like.

  As the soil-habiting phytopathogenic fungi, those similar to the above can be used.

  Giving useful microorganisms, soil pH-correcting substances, field soil, test substances, and / or soil-inhabiting phytopathogenic fungi to seeded or cultivated soil can be done by mixing, irrigating, or spreading to the soil. it can. Application of useful microorganisms, test substances, and / or soil-inhabiting phytopathogenic fungi to crops, crop seeds, or crop foliage can be carried out by spraying, applying, dipping, or injecting the crops.

  In the plant disease resistance inducer testing method of the present invention, from the viewpoint of workability for handling a large number of candidate substances, the culture medium is placed in a cell tray cell having about 32 to 200 cells having a capacity of 10 to 300 ml. Put the seeds on the seeds, and then transplant the plants without transplanting them. If necessary, depending on the target crop, for example, about 1 to 6 weeks after sowing, production of plants above the border or cotyledons It is preferable to measure the weight and determine whether or not it is useful for controlling soil infectious diseases accompanied by the promotion of crop growth based on the value. The criteria for determining whether or not it is useful can be determined as appropriate.

  Hereinafter, the present invention will be described in detail with reference to examples. However, the present invention is not limited to the following examples.

<Experimental example 1>
In order to evaluate the effects of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious diseases, melon cultivation was conducted in a glass laboratory.

Melon (variety: Kintaro) was sown as a cultivated plant in a 128-well cell tray packed with seedling culture soil (trade name “JA Nippi Horticultural Culture No. 1”, manufactured by Nippon Fertilizer Co., Ltd.). Immediately after seeding, the test group to be treated with useful microorganisms was inoculated with 5 ml of a suspension of yeast-like cells (bud cells) of Fusarium oxysporum SL0037 (2 × 10 ⁷ cells / ml) as a useful microorganism. did. Eleven days after sowing, the test group for inducing resistance to plant diseases includes “Stripe oryzate granule wettable powder” (trade name, manufactured by Meiji Seika Pharma Co., Ltd., active ingredient: probenazole), “Buiget floorable” (trade name, Nippon Pesticide Co., Ltd., active ingredient: thiazinyl agent), “validacin solution 5” (trade name, manufactured by Sumitomo Chemical Co., Ltd., active ingredient: validamycin A), or “lentemin solution” (trade name, Noda Shokubai Kogyo Co., Ltd.) Manufactured, active ingredient: shiitake mycelium culture medium extract) was irrigated into the culture medium in the cell. 15 days after sowing, mixed soil of field soil, Nippi Horticultural soil No. 1 and vermiculite inoculated with Fusarium oxysporum f. Sp. Melonis as soil pathogen (mass ratio 2: 2: 1, density of melon vine split fungus) 5 × 10 ⁶ cells / g) were transplanted into a size 10.5 cm polypot. At this time, in the test group to correct soil pH, the powdered temperament lime (trade name, manufactured by Minex Co., Ltd.), which is a converter slag material, was mixed with the soil (61 g / kg, target pH 7.5) . 15 days after transplantation, as an index to evaluate the effect on the incidence of melon vine split disease, a disease index indicating the degree of external symptom of melon vine split disease was established (0, no disease; 1, yellowing; 2, this Leaf browning; 3, withering), and each melon strain was classified according to the severity of the disease. Table 6 shows the degree of occurrence of melon vine split disease in each treatment area.

  As shown in Table 6, in the untreated test group, external symptom of melon vine split disease occurred in all melon strains tested, and the average disease index was 2.0. Although the mean disease index was improving in the test group treated with useful microorganisms and the test group treated with useful microorganisms and soil pH correction, at least half of the melon strains had external symptoms of melon split disease. The improvement effect was insufficient. On the other hand, in addition to the treatment of useful microorganisms and soil pH correction, "Side oryzate granule wettable powder" (active ingredient: probenazole), "Vuget flowable" (active ingredient: thiazinyl agent), "Varidacin solution 5" In the test group in which resistance was induced by (active ingredient: validamycin A) or “lentemine solution” (active ingredient: shiitake mycelium culture medium extract), the number of plants causing external symptoms almost disappeared. The disease index improved further. Therefore, it is more effective than treating cultivated soil by combining useful microorganism treatment, soil pH correction and plant disease resistance induction, compared to treating them alone or treating them in combination. It became clear that soil-borne diseases could be controlled.

<Experimental example 2>
In order to evaluate the influence of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious diseases, field experiments of melon cultivation were conducted.

On June 14, 2012, melon (variety: Kintaro) was cultivated as a cultivated plant in a 128-well cell tray filled with seedling culture soil (trade name “JA Nippi Horticultural Culture No. 1”, manufactured by Nippon Fertilizer Co., Ltd.) Sowing. Immediately after seeding, the test group to be treated with useful microorganisms was inoculated with 5 ml of a suspension of yeast-like cells (bud cells) of Fusarium oxysporum SL0037 (2 × 10 ⁷ cells / ml) as a useful microorganism. did. Eleven days after sowing, the plant was transplanted (potted) into a polypot (TO polypot, size 12 cm) using JA Nippi Horticultural Culture No. 1. At this time, in the test group to correct the soil pH, the powdered temperament lime (trade name, manufactured by Minex Co., Ltd.), which is a converter slag material, was mixed with the soil (18 g / kg, target pH 7.5). . Immediately after potting, in the test group for treating useful microorganisms, 200 ml irrigated with the yeast-like cell suspension (6 × 10 ⁶ cells / ml) of the SL0037 strain again as a useful microorganism in the soil in each pot Vaccinated. Twelve days after potting, the test group for inducing resistance to plant diseases included irrigation treatment with 120 g of a 0.1 g / l suspension of “Stripe oryzate granule wettable powder” (active ingredient: probenazole) in each pot medium (120 ml). / Pot). After 14 days of potting (July 10 of the same year), the plants were planted in a field (5 × 10 ⁴ cells / g) pre-inoculated with melon vines (Fusarium oxysporum f. Sp. Melonis) as a soil pathogen. From the 24th day after planting, melon strains that grew more than 24th section were culled in this section. The main stem length and fruit weight of melon after planting were measured as indices for evaluating the influence on the occurrence of melon vine split disease. The main stem length was the length of the main stem above the cotyledonary nodes, and was measured after 16, 23, 30, 36, 44, and 50 days after planting. Moreover, the melon fruit weight was made into the fresh weight immediately after collection | collection, and the fruit which most of the surface changed to yellow after planting 50-63 days was measured.

In FIG. 1, the measurement result of the main stem length is shown. Table 7 shows the test results for the presence or absence of synergistic effects on the main stem length. The test for the presence or absence of synergistic effect is based on the predicted decrease in EV (%) of the main stem length when there is no treatment compared to the combination treatment, according to Colby's theory (Colby, RS 1967 Weeds 15: 20-22). In addition, it was determined using the following calculation formula, and this was compared with the actually measured growth reduction rate OV (%). When the measured value was smaller than the predicted value, it was determined that there was a synergistic action, when both values were equal, an additive action, and when the measured value was larger than the predicted value, there was a canceling action.
-Predicted value when there are two types of combination processing: EV = x + y-xy / 100 (1)
-Predicted value when there are 3 types of combination processing: EV = x + y + z-(xy + xz + yz) / 100 + xyz / 10000… (2)
(In the above formula (1) or (2), x is an actual measurement value of the growth reduction rate when the single treatment of the useful microorganism treatment is performed, and y is the growth reduction rate when the single treatment of soil pH correction is performed. Z is an actual measured value of the rate of growth reduction when a single treatment for plant disease resistance induction is performed.)

  As shown in FIG. 1, after 23 days after planting, the main stem length became the longest when useful microorganism treatment, soil pH correction, and plant disease resistance induction were all combined. Moreover, as shown in Table 7, as a result of the test, the action effect of main stem length elongation by treatment of useful microorganisms, soil pH correction and plant disease resistance induction is synergistic action effect by the combination of these three treatments. It became clear that.

  In FIG. 2, the measurement result of fresh fruit weight is shown. In the evaluation of the result of measuring the fresh fruit weight, the fruits collected from each melon strain are ranked in order of the largest fresh weight, and when only the heaviest fruit is targeted, the heaviest fruit and the second heaviest fruit are targeted. In this case, the evaluation pattern was divided when the first to third heaviest fruits were targeted, the first to fourth heaviest fruits were targeted, and the first to fifth heaviest fruits were targeted. In addition, in the case of melon strains where the number of fruits that could be collected was less than 5, the calculation was performed assuming that the missing fruit weight was 0 g. On the other hand, Table 8 shows the test results for the presence or absence of synergistic effects on the fresh fruit weight, which were tested in the same manner as for the main stem length.

  As shown in FIG. 2, in all the evaluation patterns, when the useful microorganism treatment, soil pH correction, and plant disease resistance induction were all combined, the fruit weight became heaviest. Moreover, as shown in Table 8, as a result of the test, the effect of increasing the fresh fruit weight by the treatment of useful microorganisms, the correction of soil pH and the induction of plant disease resistance is a synergistic effect of the combination of these three treatments. It became clear that.

  The above results show that when all the useful microorganism treatment, soil pH correction, and plant disease resistance induction are combined, the control effect of melon vine split disease is the highest. The weight was thought to have increased. Therefore, it is more effective than treating cultivated soil by combining useful microorganism treatment, soil pH correction and plant disease resistance induction, compared to treating them alone or treating them in combination. It became clear that soil-borne diseases could be controlled. In addition, it is clear that if the treatment is carried out during the sowing and seedling season of the crop, even if it is subsequently planted in a field where the pathogenic fungi of the soil are present, the effect of disease control can be obtained effectively. It became.

<Experimental example 3>
In order to evaluate the effects of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious diseases, a small-scale laboratory experiment (simplified test) of melon cultivation was conducted.

One cell of melon (variety: Kintaro) in a 50-well cell tray (8 cells per experimental zone) packed with seedling culture soil (brand name “JA Nippi Horticultural Culture No. 1”, manufactured by Nippon Fertilizer Co., Ltd.) 4 seeds per seed. Immediately after seeding, the test group to be treated with useful microorganisms was inoculated with 16.2 ml of a yeast cell-like suspension (2 × 10 ⁷ cells / ml) of Fusarium oxysporum SL0037 as a useful microorganism in each cell. did. Six days after sowing, two melon seedlings were thinned out per cell, and soil suspension (150 g / l) was irrigated into the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Similarly, 6 days after sowing, the test group to correct the soil pH contains 165.6 g / l of a suspension of “powdered lime lime” (trade name, manufactured by Minex Co., Ltd.), which is a converter slag material, in each cell. The medium was irrigated (8.2 ml / cell). Nine days after sowing, the test group for inducing plant disease resistance was irrigated with a 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the medium in each cell (8.2 ml / cell). Thirteen days after sowing, a yeast-like cell suspension (8 × 10 ⁷ cells / ml) of Fusarium oxysporum f. Sp. (3 ml / plant). 27 days after sowing, the raw weight of the plant body (including cotyledons) above the cotyledon node was measured as an index for evaluating the influence on the degree of occurrence of melon vine split disease. The results are shown in FIG.

  As shown in FIG. 3, when the useful microorganism treatment, soil pH correction, and plant disease resistance induction were all combined, the fresh weight of the melon plant was the highest.

  Based on the above results, it is considered that melon plants grew greatly in the test group because the effective control of melon vine split disease was the highest when combined with useful microorganism treatment, soil pH correction, and plant disease resistance induction. It was. Therefore, the tendency of phenomena observed in the field can be reproduced even in a small-scale laboratory experiment, and by using a simple test that can perform all the processes in the cell tray, another useful microorganism strain, soil pH correcting substance, and It has become clear that it is possible to screen combinations of inducers of plant disease resistance.

<Experimental example 4>
In order to evaluate the effects of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the growth of crops, melon cultivation in a net experiment was conducted twice. However, in the second experiment, crops were moved to the glass room 32 days after sowing in order to reduce the influence of temperature due to seasonal changes.

Melon (variety: Kintaro) was sown as a cultivated plant in a 128-well cell tray packed with seedling culture soil (trade name “JA Nippi Horticultural Culture No. 1”, manufactured by Nippon Fertilizer Co., Ltd.). Immediately after sowing, a test group that combines useful microorganism treatment, soil pH correction, and plant disease resistance induction includes a yeast-like cell (bud cell) suspension of Fusarium oxysporum SL0037 strain (2 × 10 ⁷ cells / ml). ) Was inoculated into 5 ml of soil in each cell. Seven days after sowing, the plant was transplanted (potted) into a polypot (TO polypot, size 9 cm) using JA Nippi Horticultural Culture No. 1. At this time, in the test group that combines useful microorganism treatment, soil pH correction, and plant disease resistance induction, “Powdered lime lime” (trade name, manufactured by Minex Co., Ltd.), which is a converter slag material, is mixed with the soil. (18 g / kg, target pH 7.5). Immediately after potting, the test group combining useful microorganism treatment, soil pH correction, and plant disease resistance induction again included a yeast-like cell suspension (6 × 10 ⁶ cells / cell) of the SL0037 strain as a useful microorganism. ml) was inoculated (87 ml / pot) into the soil in each pot. In the first experiment, after 13 days of potting, in the second experiment, after 11 days of potting, the test group combining useful microorganism treatment, soil pH correction, and induction of plant disease resistance was classified as “Striped oryzate granule wettable powder”. A 0.1 g / l suspension of (active ingredient: probenazole) was irrigated (52 ml / pot) into each pot medium. In the first experiment, after 17 days of potting, in the second experiment, after 15 days of potting, a Wagner pot (1: 40: 40: 9) mixed with field soil, JA Nippi Horticultural soil No. 1 and vermiculite (1 to 40) is used. / 5000 are). As an index to evaluate the effects on the growth of crops, we measured the main stem length of melon after Wagner pot transplantation and counted the nodes of the main stem. The length of the main stem is the length of the main stem above the cotyledonary node. In the first experiment, 6, 13, 20, 27, 34, 41, 48 days after transplantation, the transplantation in the second experiment is 7, 11, 14, 17 , 21, 24, 29, 32, 37 days later. The first node is the cotyledon node, 20, 27, 34, 41, 48 days after transplantation in the first experiment, and 7, 11, 14, 17, 21, 24, 32, 37 days after transplantation in the second experiment. I counted it later. The results are shown in FIG. In the first experiment, a photograph of a melon plant was taken 41 days after transplantation. A photograph is shown in FIG.

  As shown in FIGS. 4 and 5, in the test group combining useful microorganism treatment, soil pH correction, and plant disease resistance induction, the main stem grew faster than the untreated test group. The increase in the number of nodes was faster in the test group combining useful microorganism treatment, soil pH correction, and plant disease resistance induction than in the untreated test group. Therefore, the growth of melon plants was promoted by combining useful microorganism treatment, soil pH correction and plant disease resistance induction. Also. If treated during the sowing and seedling periods of crops, even if planted in a field where there are no pathogenic bacteria of soil infectious diseases after that, shortening the cultivation period by promoting growth and further increasing the yield can be obtained It became clear.

<Experimental example 5>
In order to evaluate the effect of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious disease, a small-scale indoor experiment (simple test) of tomato cultivation was conducted.

In the test group that treats useful microorganisms, "Twin Guard" (trade name, manufactured by Idemitsu Kosan Co., Ltd.), a microorganism material, is used for raising seedlings (trade name "JA Nippi Horticultural Culture No. 1", manufactured by Nippon Fertilizer Co., Ltd.) To 100 g / kg. In the test group for correcting soil pH, “powdered lime lime” (trade name, manufactured by Minex Co., Ltd.), which is a converter slag material, was mixed with the soil so as to be 18 g / kg. After cultivating soil in a 50-well cell tray, 4 tomatoes (variety: yellow pair) were sown per cell. In addition, 8 cells for each experimental section were used. Six days after sowing, two tomato seedlings were thinned out per cell, and soil suspension (150 g / l) was irrigated into the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Nine days after sowing, the test group for inducing plant disease resistance was irrigated with a 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the soil in each cell ( 8.2 ml / cell). 13 days after sowing, a suspension (4 × 10 ⁸ cfu / ml) of tomato bacterial wilt (Ralstonia solanacearum) as a soil pathogen was irrigated and inoculated into the soil in the vicinity of each tomato plant (3 ml / plant). 27 days after sowing, the raw weight of the plant body (including cotyledons) above the cotyledon node was measured as an index for evaluating the effect on the degree of occurrence of tomato bacterial wilt. The results are shown in FIG.

  As shown in FIG. 6, the raw weight of the tomato plant was the highest when the treatment with microbial material, soil pH correction, and plant disease resistance induction were all combined.

  Based on the above results, it is considered that tomato plants grew greatly in the test group because the control effect of tomato bacterial wilt is the highest when combined with useful microorganism treatment for soil pH correction and plant disease resistance induction. It was.

  “Twinguard” is a microbial material containing mycorrhizal fungi and Gliocladium spp. Therefore, both mycorrhizal fungi and genus Gliocladium, or any one of them, are useful to control soil infectious diseases more effectively by treating soil in combination with soil pH correction and plant disease resistance induction It became clear that it was a microorganism.

<Experimental example 6>
In order to evaluate the effects of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the occurrence of disease in the presence of high-density soil pathogens, indoor small-scale experiments (simple test) ).

In the test group that corrects the soil pH, the converter slag material “Powdered lime” (trade name, manufactured by Minex Co., Ltd.) is used for raising seedlings (trade name “JA Nippi Horticultural Culture No. 1”), Japanese fertilizer (Made by Co., Ltd.) was mixed so as to be 18 g / kg. As the useful microorganism treatment, “Twinguard” (trade name, manufactured by Idemitsu Kosan Co., Ltd.) or “Bottokiller wettable powder” (trade name, manufactured by Idemitsu Kosan Co., Ltd., active ingredient: Bacillus subtilis), which is a microbial material, was used. . In the test group in which “Twin Guard” was tested, this microbial material was mixed at 100 g / kg in the soil, packed in a 50-well cell tray, and then 4 tomatoes were sown per cell. In the test group that tested the “Bottokiller wettable powder”, four tomatoes (variety: yellow pair) were seeded per cell in a 50-well cell tray (8 cells per experimental area) packed with soil. A 16.2 ml irrigation treatment of 1000 times diluted solution of “Bottokiller wettable powder” was carried out on the soil in each cell. In addition, 8 cells for each experimental section were used. Six days after sowing, two tomato seedlings were thinned out per cell, and soil suspension (150 g / l) was irrigated into the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Nine days after sowing, the test group for inducing plant disease resistance was irrigated with a 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the soil in each cell ( 8.2 ml / cell). Thirteen days after sowing, a suspension (3 × 10 ⁹ cfu / ml) of a tomato bacterial wilt fungus (Ralstonia solanacearum) having a higher density than that of Experimental Example 5 as a soil pathogen was irrigated and inoculated into the soil around each tomato plant (3 ml). /plant). 25 days after sowing, the raw weight of the plant body (including cotyledons) above the cotyledon node was measured as an index for evaluating the influence on the occurrence of tomato bacterial wilt. The results are shown in FIG.

  As shown in FIG. 7, when "Twingard" or "Bottokiller wettable powder" is treated, the tomato is compared with the case where soil pH correction and plant disease resistance induction are each treated alone and when these two kinds are combined. The raw weight of the plant was high.

  Based on the above results, it is considered that tomato plants grew greatly in the test group because the control effect of tomato bacterial wilt is the highest when combined with useful microorganism treatment for soil pH correction and plant disease resistance induction. It was.

  “Twinguard” is a microbial material containing mycorrhizal fungi and Gliocladium spp. Therefore, both mycorrhizal fungi and / or Gliocladium spp. Can be used to treat soil in combination with soil pH correction and plant disease resistance induction, even when soil pathogens are present in high density. It became clear that it is a useful microorganism that can control the disease caused. “Bottokiller wettable powder” is a microbial pesticide containing Bacillus subtilus. Therefore, it is clear that this inoculum is a useful microorganism that can control diseases caused by soil pathogens even when they are present at high density by treating soil in combination with soil pH correction and plant disease resistance induction. It became.

<Experimental example 7>
In order to evaluate the effect of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious disease, a small-scale indoor experiment (simple test) of tomato cultivation was conducted.

In the test group that treats useful microorganisms, "Twin Guard" (trade name, manufactured by Idemitsu Kosan Co., Ltd.), a microorganism material, is used for raising seedlings (trade name "JA Nippi Horticultural Culture No. 1", manufactured by Nippon Fertilizer Co., Ltd.) To 100 g / kg. In the test group for correcting the soil pH, “70 slaked lime” (trade name, manufactured by Toa Sangyo Co., Ltd., alkali content: 70%), which is a slaked lime material, was mixed with the soil so as to be 2.5 g / kg. After cultivating soil in a 50-well cell tray, 4 tomatoes (variety: yellow pair) were sown per cell. In addition, 8 cells for each experimental section were used. Six days after sowing, two tomato seedlings were thinned out per cell, and soil suspension (150 g / l) was irrigated into the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Nine days after sowing, the test group for inducing plant disease resistance was irrigated with a 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the soil in each cell ( 8.2 ml / cell). 13 days after sowing, a suspension (3 × 10 ⁹ cfu / ml) of tomato bacterial wilt (Ralstonia solanacearum) was soil-inoculated as a soil pathogen (3 ml / plant). 21 days after sowing, the raw weight of the plant body (including cotyledons) above the cotyledon node was measured as an index for evaluating the effect on the degree of occurrence of tomato bacterial wilt. The results are shown in FIG.

  As shown in FIG. 8, when slaked lime material is combined with useful microorganism treatment and plant disease resistance induction, when slaked lime material is used alone and when useful microorganism treatment and plant disease resistance induction are combined, Compared with the fresh weight of the tomato plant.

  The above results show that tomato plant wilt is most effective when combined with treatment of useful microorganisms and induction of plant disease resistance in combination with slaked lime material treatment as a soil pH correction. It was thought that it was. Therefore, it became clear that slaked lime material is a soil pH correction substance effective for soil infectious diseases combined with useful microorganism treatment and plant disease resistance induction.

<Experimental Example 8>
In order to evaluate the effect of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious disease, a small-scale indoor experiment (simple test) of tomato cultivation was conducted.

In the test group that treats useful microorganisms, "Twin Guard" (trade name, manufactured by Idemitsu Kosan Co., Ltd.), a microorganism material, is used for raising seedlings (trade name "JA Nippi Horticultural Culture No. 1", manufactured by Nippon Fertilizer Co., Ltd.) To 100 g / kg. After cultivating soil in a 50-well cell tray, 4 tomatoes (variety: yellow pair) were sown per cell. Immediately after sowing, the test group for correcting the soil pH was irrigated with 16.4 ml of calcium carbonate (CaCO ₃ ) suspension (10.81 g / l) in the soil in each cell. In addition, 8 cells for each experimental section were used. Six days after sowing, two tomato seedlings were thinned out per cell, and soil suspension (150 g / l) was irrigated into the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Nine days after sowing, the test group for inducing plant disease resistance was irrigated with a 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the medium in each cell (8.2 ml / cell). 13 days after sowing, a suspension (3 × 10 ⁹ cfu / ml) of tomato bacterial wilt (Ralstonia solanacearum) was soil-inoculated as a soil pathogen (3 ml / plant). Twenty-two days after sowing, the raw weight of plants (including cotyledons) above the cotyledon nodes was measured as an index for evaluating the effect on the occurrence of tomato bacterial wilt. The results are shown in FIG.

  As shown in FIG. 9, when calcium carbonate is combined with useful microorganism treatment and plant disease resistance induction, when calcium carbonate is carried out alone and when useful microorganism treatment and plant disease resistance induction are combined, Compared with the fresh weight of the tomato plant.

  The above results indicate that when the treatment with useful microorganisms and the induction of plant disease resistance are combined with calcium carbonate treatment as a soil pH correction, the tomato bacterial wilt disease control effect is most enhanced. It was thought that it was. Therefore, it became clear that calcium carbonate is an effective soil pH correcting substance for soil infectious diseases combined with treatment of useful microorganisms and induction of plant disease resistance.

<Experimental Example 9>
In order to evaluate the effects of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious diseases, a small-scale laboratory experiment (simplified test) of melon cultivation was conducted.

In the test group that corrects the soil pH, the converter slag material “Powdered lime” (trade name, manufactured by Minex Co., Ltd.) is used for raising seedlings (trade name “JA Nippi Horticultural Culture No. 1”), Japanese fertilizer (Made by Co., Ltd.) was mixed so as to be 18 g / kg. After filling the culture soil in a 50-well cell tray, 4 melon (variety: Amus) seeded per cell. Immediately after sowing, the test group for treatment of useful microorganisms includes a 200-fold diluted solution of “Tough Block” (trade name, manufactured by Idemitsu Kosan Co., Ltd., active ingredient: Talaromyces flavus SAY-Y-94-01) in each cell. The medium was inoculated with 16.2 ml of irrigation. In addition, 8 cells for each experimental section were used. Six days after sowing, two melon seedlings were thinned out per cell, and soil suspension (150 g / l) was irrigated into the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Nine days after sowing, the test group for inducing plant disease resistance was irrigated with a 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the soil in each cell ( 8.2 ml / cell). Thirteen days after sowing, a yeast-like cell suspension (8 × 10 ⁷ cells / ml) of Fusarium oxysporum f. Sp. (3 ml / plant). 27 days after sowing, the raw weight of the plant body (including cotyledons) above the cotyledon node was measured as an index for evaluating the influence on the degree of occurrence of melon vine split disease. The results are shown in FIG.

  As shown in FIG. 10, compared with the case where soil pH correction and plant disease resistance induction were combined, the raw weight of the melon plant was increased by combining the treatment with “tough block”.

  From the above results, it was considered that the melon plant grew greatly in the test group because the control effect of melon vine split disease was enhanced when combined with useful microorganism treatment for soil pH correction and plant disease resistance induction. . Therefore, it was revealed that Talaromyces flavus contained as an active ingredient of “tough block” is a useful microorganism effective for controlling soil infectious diseases in combination with soil pH correction and plant disease resistance induction.

<Experimental example 10>
In order to evaluate the effect of the combination of useful microorganism treatment, soil pH correction, and plant disease resistance induction on the degree of occurrence of soil infectious diseases, a small-scale indoor experiment (simplified test) of cabbage cultivation was conducted.

In the test group that corrects the soil pH, the converter slag material “Powdered lime” (trade name, manufactured by Minex Co., Ltd.) is used for raising seedlings (trade name “JA Nippi Horticultural Culture No. 1”), Japanese fertilizer (Made by Co., Ltd.) and mixed to 20 g / kg. After cultivating soil in a 50-well cell tray, 4 seeds of cabbage (variety: seasonal harvest) were sown per cell. Immediately after sowing, the test group to be treated with useful microorganisms was inoculated with 16.2 ml of a yeast-like cell (bud cell) suspension (2 × 10 ⁷ cells / ml) of Fusarium oxysporum SL0037 in the medium in each cell. In addition, 8 cells for each experimental section were used. 14 days after sowing, 2 cabbage seedlings per cell were thinned out, and soil suspension (150 g / l) was irrigated to the soil in each cell (8.2 ml / cell) for the purpose of bringing them closer to the field conditions. ). Sixteen days after sowing, the test group for inducing plant disease resistance was irrigated with 0.2 g / l suspension of “Striped oryzate granule wettable powder” (active ingredient: probenazole) in the soil in each cell ( 8.2 ml / cell). 20 days after sowing, a yeast-like cell (bud cell) suspension (6 × 10 ⁷ cells / ml) of Fusarium oxysporum f. Sp. (3 ml / plant). 34 days after sowing, the raw weight of the plant body (including cotyledons) above the cotyledon node was measured as an index for evaluating the effect on the occurrence of cabbage wilt. The results are shown in FIG.

  As shown in FIG. 11, the raw weight of the cabbage was higher when the useful microorganism treatment, soil pH correction, and plant disease resistance induction were combined.

  From the above results, it was considered that cabbage grew greatly in the test group because the control effect of cabbage wilt yellow was the highest when combined with useful microorganism treatment, soil pH correction and induction of plant disease resistance. Therefore, the combination of `` Fusarium oxysporum SL0037 strain '', `` powdered limestone lime '' and `` side stripe oryzate granule wettable powder '' which was effective in controlling melon vine split disease is also effective in controlling cabbage wilt yellow. It became clear that there was.

Claims (7)

Gliocladium, Talaromyces, Fusarium, Erwinia, Pseudomonas, Bacillus, and mycorrhizal fungi during sowing and seedling of crops Giving soil disease or crops with a plant disease resistance-inducing substance consisting of microorganisms and probenazole for controlling soil infectious diseases including one or more selected from the group consisting of , and soil pH correction to increase pH by application to the soil material, soil壌伝dyeable disease control method you characterized by controlling bacterial or fungal soil-borne disease. In the process of sowing, raising seedlings, and planting of crops, the seedlings that have been germinated are transplanted from the seeding floor to pots to grow seedlings, and then planted in the field, the above-mentioned microorganisms for controlling soil infectious diseases are seeded. given to the crop seed sowing soil or disseminated at the time of the period until the pot up from time to time, further, again also given to soil in the pot of the period from the time of pots until planting in the field, or pot from the time of seeding It is applied only to the sowing soil for the period until sowing or the crop seed at the time of sowing, and the soil pH-correcting substance is applied to the sowing soil for the period from sowing to pot raising, and further planted in the field from the time of potting. again applied to soil in pots period until or grants from the time of potting only soil in pots period until the planting in the field, the field of the plant disease resistance inducer from the time of potting The period until planting Soil壌伝dyeable disease control method according to claim 1 for imparting to the soil or crops foliage in. When planting seedlings directly from the seeding floor to the field without raising pots during the sowing, raising and planting of crops, sowing of the soil infectious disease control microorganisms from the time of sowing to the time of planting granted to crop seed soil or sowing time, until the soil pH corrective agent grants from the time of sowing the seed soil period until planting in the field, is planted in the field with the plant disease resistance inducer from the time of seeding soil壌伝dyeable disease control method according to claim 1 to be applied to the crop foliage sowing soil or crop seeds or in nursery during sowing period. Take nutritionally Konae without seeding crops, seedlings and when planting in the field in the after imparts a microorganism for the soil-borne disease control in soil time to planting from the time Tonae, the Soil the pH corrective agent was applied to the soil of the period until planting from the time Tonae of claim 1 wherein applying the plant disease resistance inducer to the crop foliage in soil or nursery time to planting from the time Tonae Sat壌伝dyeable disease control method. Gliocladium, Talaromyces, Fusarium, Erwinia, Pseudomonas, Bacillus and mycorrhizal fungi during the sowing and seedling periods of crops the selected test microorganisms from the group consisting of imparting to the soil or crops, impart soil pH corrective agent for increasing the pH was applied to the soil, plant disease resistance inducer consisting probenazole to soil or crops , and then a soil-living cultivated by imparting bacterial or fungal plant pathogens in soil or crop foliage, resulting in the test microorganisms are the soil pH correction material and the plant disease resistance inducer and by Kumiaisaru, the if Ru enhances the onset inhibitory effect as compared with the case of using only the test microorganisms, the microorganisms this was subjected are any of the preceding claims 1 Method of assaying microorganisms for controlling a soil-borne disease, which comprises determining that a microorganism useful for soil壌伝dyeable disease control method described. Gliocladium, Talaromyces, Fusarium, Erwinia, Pseudomonas, Bacillus and mycorrhizal fungi during the sowing and seedling periods of crops the selected test microorganisms from the group consisting of imparting to the soil or crops, the soil pH corrective agent for increasing the pH was applied to the soil, then plant disease resistance inducer consisting probenazole to soil or crops granted to cultivation, as a result, by the test microorganisms are the soil pH correction material and the plant disease resistance inducer in combination Sarukoto, the crop growth as compared with the case of using only the test microorganisms promoted the cases that will be a determining means determines that the microorganisms this was subjected are useful microorganisms in the soil壌伝dyeable disease control method according to any one of claims 1 to 4 Assay method of that soil-borne disease control for the microorganisms. As the soil pH corrective agent, converter slag, hydrated lime, and soil壌伝dyeable disease control according to any one of claims 1-4 using one or more selected from the group consisting of calcium carbonate Law.