CN105210592A - A kind of method of Coffea Arabica cutting propagation - Google Patents
A kind of method of Coffea Arabica cutting propagation Download PDFInfo
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- CN105210592A CN105210592A CN201410294917.7A CN201410294917A CN105210592A CN 105210592 A CN105210592 A CN 105210592A CN 201410294917 A CN201410294917 A CN 201410294917A CN 105210592 A CN105210592 A CN 105210592A
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- coffea arabica
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Abstract
The present invention relates to plant pierre technical field, disclose a kind of method of Coffea Arabica cutting propagation, cultivating cuttings for adopting the method for the invention; Cuttings is dipped in 3 ~ 6s at the NAA solution of 3000mg/L; Cuttings after process is inserted in the matrix that the husky and sawdust of effluent forms, and carry out water-retaining cultivation 3 ~ 4 months, be then transferred in seedling-growing container and carry out later stage hardening and namely obtain Coffea Arabica cuttage seeding.The present invention makes Coffea Arabica adopt the survival rate be propagated from cuttings more than 80%, compensate for the deficiency that existing method rooting rate is extremely low, has comparatively far reaching significance to the direct utilization of the preservation of Coffea Arabica provenance and low generation elite hybrid.
Description
Technical field
The present invention relates to plant pierre technical field, be specifically related to a kind of method of Coffea Arabica cutting propagation.
Background technology
Coffea Arabica (CoffeaarabicaL.) is Rubiaceae (Rubiaceae) Coffea (Coffea) perennial evergreen shrub or dungarunga, also be a kind of important tropical economic crops, first of the large beverage in the world three, Coffea Arabica belongs to self-pollinated plant, though Seedling tree genetic character variability is little, but also there is the natural variation rate that the alienation pollination of about 5% ~ 10% is introduced, therefore, utilize seed growing method, preserve and low generation Hybrid fixing and there is certain defective in utilizing in germ plasm resource hereditary capacity.
Cuttage belongs to one of vegetative propagation technique, it is the common method of breeding plant, cottage propagation can the better hereditary capacity that must keep seeds or kind, and there is quick, high-quality, efficient advantage of breeding nursery stock, be widely used in the fields such as veterinary antibiotics, flower gardening material, achievements in forest-tree seedling, traditional Chinese medicine in practice.
At present, the domestic report about coffee cuttage and seedling culture is only about Coffea canephora cuttage and seedling culture method, Coffea Arabica cuttage and seedling culture is carried out by literature procedure, not easily take root, difficulty survives, therefore, a kind of Coffea Arabica cuttage and seedling culture method is provided, merit fixed and uses, existing excellent genetic stocks can be made to put into production utilization, promoting Coffea Arabica improved variety process.
Summary of the invention
The invention reside in and overcome deficiency of the prior art, thus a kind of convenient, fast and Coffea Arabica cuttage breeding method that cottage propagation survival rate is high is provided.
To achieve these goals, the invention provides following technical scheme:
A method for coffee cuttage and seedling culture, comprises the following steps:
(1) Coffea Arabica upright growth is cultivated: selected Coffea Arabica maternal plant is carried out pruning rear horizontal curved solid fixed, the sleeping bud place vertical-growth often saved goes out water sprout, is Coffea Arabica upright growth; Maybe by selected Coffea Arabica maternal plant, cut off plant top, the highest joint of this branch grows two upright growths.
(2) choose Coffea Arabica upright growth and cut out acquisition cuttings: choosing the Coffea Arabica upright growth that green, non-suberification, blade are aging, then the branch of 3 ~ 5 joints is cut into, the blade of cutting simultaneously makes each cutting retain 2 ~ 3 pairs of blades, each blade stays 1/3, then cutting is soaked 30 ~ 60s in 1000 times of carbendazim solutions, obtain cuttings.
(3) cuttage: the inclined-plane with sharp cutter, cuttings end being whittled into about miter angle, dips in 3 ~ 6s at the NAA solution of 3000mg/L, then inserts in cutting medium by cuttings, cuttings insertion depth about 6 ~ 8cm, cuttings spacing about 4 ~ 5cm.
(4) water-retaining cultivation: cover flowerpot with clear plastic wrap pack, and the container of a filled with water is placed in flowerpot central authorities, make relative moisture about 85%, cultivate 3 ~ 4 months under indoor or shade rate are the environment of 80%-90%, period kept the skin wet and humidity with water sprager atomized water spray every 3 ~ 5 days, sprays the bactericide such as 1000 times of Difenoconazoles carry out sterilization every 20 ~ 25 days.
(5) transplant hardening: moved in nutritive cube by the coffee seedling of taking root, and cover moisturizing with transparent plastic bag, be cultivate 1 ~ 2 month under the environment of about 80% at shade rate, after sending 6 pairs of young leaves, coffee nursery stock management method manages routinely.
Based on above-mentioned, the preparation of described cutting medium be by volume ratio be 2: 1 river sand and sawdust high-temperature heat sterilization after, be placed in flowerpot, water of drenching.
Adopt the inventive method cuttage breeding method to breed Coffea Arabica, its survival rate of seedling has great raising, shows through test of many times result, and this survival rate of seedling is more than 80%.
Embodiment
The invention discloses a kind of method of Coffea Arabica cuttage and seedling culture, those skilled in the art can use for reference present disclosure, suitable improving technique parameter realizes, special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention, product of the present invention and method are described by preferred embodiment, related personnel obviously can not depart from content of the present invention, in spirit and scope, methods described herein and application are changed or suitably change and combination, realize and apply the technology of the present invention.
Embodiment 1:
(1) cultivate Coffea Arabica upright growth: select Coffea Arabica maternal plant in October and prune, after pruning, level is fixed, and goes out and vertically grows water sprout, be Coffea Arabica upright growth at the sleeping bud often saved.
(2) preparation of cutting medium and container: cuttage vessel selects bore to be greater than the flowerpot of 40cm, the cutting medium employing volume ratio in flowerpot is river sand and the sawdust of 2: 1, and matrix carries out high-temperature heat sterilization process in first 2 days in cuttage, the thick about 30cm of matrix.
(3) cuttings preparation: next year by the end of March, choose the upright growth that green, non-suberification, blade are aging, be cut into the branch of 3 joints, cutting blade makes each cutting retain 2 pairs of blades, each blade stays 1/3, then cutting is soaked 30 ~ 60s in 1000 times of carbendazim solutions, then with cutter, cuttings end is whittled into the inclined-plane of about miter angle, after the NAA solution of 3000mg/L dips in 3 ~ 6s, namely obtain cuttings.
(4) cuttage: punch on cutting medium with waddy, insert cuttings, the cuttage degree of depth is about 5cm, and cuttings spacing is about 5cm, compresses and drench sufficient moisture after cuttage.
(5) water-retaining cultivation: cover flowerpot with clear plastic wrap pack, and the container of a filled with water is placed in flowerpot central authorities, make relative moisture about 85%, about 3 first quarter moons are cultivated under indoor or shade rate are the environment of 80% ~ 90%, period kept the skin wet and humidity with water sprager atomized water spray every 3 ~ 5 days, sprays the bactericide such as 1000 times of Difenoconazoles carry out sterilization every 20 ~ 25 days.
(6) transplant hardening: moved in nutritive cube by the coffee seedling of taking root, and cover moisturizing with transparent plastic bag, be cultivate about 1 first quarter moon under the environment of about 80% at shade rate, after sending 6 young leaves, coffee nursery stock management method manages routinely.
Through test in 3 years, cuttage seeding average survival was more than 85%, and seedlings root is flourishing, and after transplanting, survival rate is more than 95%, and growing way is good.
Embodiment 2:
(1) cultivate Coffea Arabica upright growth: select Coffea Arabica maternal plant in February, cut off plant top, the highest joint of this branch grows two upright growths, is Coffea Arabica upright growth.
(2) preparation of cutting medium and container: cuttage vessel selects bore to be greater than the flowerpot of 40cm, the cutting medium employing volume ratio in flowerpot is river sand and the sawdust of 2: 1, and matrix carries out high-temperature heat sterilization process in first 2 days in cuttage, the thick about 30cm of matrix.
(3) cuttings preparation: next year is by the end of May to mid-June, choose the upright growth that green, non-suberification, blade are aging, be cut into the branch of 5 joints, cutting blade makes each cutting retain 2 ~ 3 pairs of blades, each blade stays 1/3, then cutting is soaked 30 ~ 60s in 1000 times of carbendazim solutions, then with cutter, cuttings end is whittled into the inclined-plane of about miter angle, after the NAA solution of 3000mg/L dips in 3 ~ 6s, namely obtain cuttings.
(4) cuttage: punch on cutting medium with waddy, insert cuttings, the cuttage degree of depth is about 7cm, and cuttings spacing is about 6cm, compresses and drench sufficient moisture after cuttage.
(5) water-retaining cultivation: cover flowerpot with clear plastic wrap pack, and the container of a filled with water is placed in flowerpot central authorities, make relative moisture about 85%, about 3 months are cultivated under indoor or shade rate are the environment of 80% ~ 90%, period kept the skin wet and humidity with water sprager atomized water spray every 3 ~ 5 days, sprays the bactericide such as 1000 times of Difenoconazoles carry out sterilization every 20 ~ 25 days.
(6) transplant hardening: moved in nutritive cube by the coffee seedling of taking root, and cover moisturizing with transparent plastic bag, be cultivate 1 month under the environment of about 80% at shade rate, after sending 6 young leaves, coffee nursery stock management method manages routinely.
Through test in 3 years, cuttage seeding average survival was more than 85%, and seedlings root is flourishing, and after transplanting, survival rate is more than 95%, and growing way is good.
Embodiment 3:
(1) cultivate Coffea Arabica upright growth: select Coffea Arabica maternal plant in June, cut off plant top, the highest joint of this branch grows two upright growths, is Coffea Arabica upright growth.
(2) preparation of cutting medium and container: cuttage vessel selects bore to be greater than the flowerpot of 40cm, the cutting medium employing volume ratio in flowerpot is river sand and the sawdust of 2: 1, and matrix carries out high-temperature heat sterilization process in first 2 days in cuttage, the thick about 30cm of matrix.
(3) cuttings preparation: next year is by the end of September to mid-October, choose the upright growth that green, non-suberification, blade are aging, be cut into the branch of 3 ~ 5 joints, cutting blade makes each cutting retain 2 ~ 3 pairs of blades, each blade stays 1/3, then cutting is soaked 30 ~ 60s in 1000 times of carbendazim solutions, then with cutter, cuttings end is whittled into the inclined-plane of about miter angle, after the NAA solution of 3000mg/L dips in 3 ~ 6s, namely obtain cuttings.
(4) cuttage: punch on cutting medium with waddy, insert cuttings, the cuttage degree of depth is about 5 ~ 8cm, and cuttings spacing is about 5 ~ 6cm, compresses and drench sufficient moisture after cuttage.
(5) water-retaining cultivation: cover flowerpot with clear plastic wrap pack, and the container of a filled with water is placed in flowerpot central authorities, make relative moisture about 85%, cultivate 3 ~ 4 months under indoor or shade rate are the environment of 80% ~ 90%, period kept the skin wet and humidity with water sprager atomized water spray every 3 ~ 5 days, sprays the bactericide such as 1000 times of Difenoconazoles carry out sterilization every 20 ~ 25 days.
(6) transplant hardening: moved in nutritive cube by the coffee seedling of taking root, and cover moisturizing with transparent plastic bag, be cultivate 2 months under the environment of about 80% at shade rate, after sending 6 young leaves, coffee nursery stock management method manages routinely.
Through test in 3 years, cuttage seeding average survival was more than 80%, and seedlings root is flourishing, and after transplanting, survival rate is more than 90%, and growing way is good.
Claims (7)
1. a method for coffee cuttage and seedling culture, is characterized in that, comprises the following steps:
Step 1, cultivation Coffea Arabica upright growth;
Step 2, choose Coffea Arabica upright growth and cut out acquisition cuttings;
Step 3, cuttings is dipped in 3s ~ 6s at the NAA solution of 3000mg/L;
Step 4, step 3 is processed after cuttings insert in cutting medium;
Step 5, water-retaining cultivation;
Namely step 6, transplanting hardening obtain coffee cuttage seeding.
2. method according to claim 1, it is characterized in that, step 1 is:
It is solid fixed that selected Coffea Arabica maternal plant carries out pruning rear horizontal curved, and the sleeping bud place vertical-growth often saved goes out water sprout, is Coffea Arabica upright growth.
3. method according to claim 1, it is characterized in that, step 1 is:
Selected Coffea Arabica maternal plant, cuts off plant top, and the highest joint of this branch grows two upright growths.
4. method according to claim 1, it is characterized in that, step 2 is:
Choose the Coffea Arabica upright growth that green, non-suberification, blade are aging, be cut into the branch of 3 ~ 5 joints, the blade of cutting simultaneously makes each cutting retain 2 ~ 3 pairs of blades, and each blade stays 1/3, remainder cuts, and namely the cutting end inclined-plane that sharp cutter is whittled into about miter angle obtains cuttings.
5. method according to claim 1, is characterized in that, matrix described in step 4 by volume ratio be 2: 1 river sand and sawdust form.
6. method according to claim 1, it is characterized in that, step 5 is:
Flowerpot is covered with clear plastic wrap pack, and the container of a filled with water is placed in flowerpot central authorities, make relative moisture about 85%, cultivate 3 ~ 4 months under indoor or shade rate are the environment of 80% ~ 90%, period kept the skin wet and humidity with water sprager atomized water spray every 3 ~ 5 days.
7. method according to claim 1, step 6 is:
Moved in nutritive cube by the coffee seedling of taking root, and cover moisturizing with transparent plastic bag, be cultivate 1 ~ 2 month under the environment of about 80% at shade rate, then coffee nursery stock management method manages routinely.
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| CN201410294917.7A CN105210592A (en) | 2014-06-17 | 2014-06-17 | A kind of method of Coffea Arabica cutting propagation |
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| CN201410294917.7A CN105210592A (en) | 2014-06-17 | 2014-06-17 | A kind of method of Coffea Arabica cutting propagation |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934585A (en) * | 2012-12-04 | 2013-02-20 | 中国热带农业科学院香料饮料研究所 | Coffee cutting seedling culture method |
| FR2994055A1 (en) * | 2012-08-03 | 2014-02-07 | Zhan-Wen Liao | METHOD FOR RAPIDLY PUSHING A TREE |
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- 2014-06-17 CN CN201410294917.7A patent/CN105210592A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2994055A1 (en) * | 2012-08-03 | 2014-02-07 | Zhan-Wen Liao | METHOD FOR RAPIDLY PUSHING A TREE |
| CN102934585A (en) * | 2012-12-04 | 2013-02-20 | 中国热带农业科学院香料饮料研究所 | Coffee cutting seedling culture method |
Non-Patent Citations (4)
| Title |
|---|
| PURUSHOTHAM等: "咖啡徒长枝的生化状况与插条生根的关系", 《热带农业科技》 * |
| 刘佩云等: "云南小粒种咖啡栽培技术的研究", 《经济林研究》 * |
| 张籍香等: "中粒种咖啡扦插试验简报", 《中国热带农业》 * |
| 李慧敏等: "小粒种、中粒种、大粒种咖啡扦插生根研究", 《第十六届中国科协年会——分17精品咖啡豆认证与公平交易及庄园标准化国际论坛论文集》 * |
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Application publication date: 20160106 |