CN105194286A - Radiation-proof external skin-care composition and essence with skin barrier repair effect and preparation method thereof - Google Patents
Radiation-proof external skin-care composition and essence with skin barrier repair effect and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a radiation-proof external skin-care composition with a skin barrier repair effect and a preparation method thereof. The radiation-proof external skin-care composition comprises the following raw materials by weight percent: 2.0-5.0 percent of a traditional Chinese medicine extract, 0.2-2.0 percent of an ammonium acryloyldimethyltaurate/VP copolymer, 0.01-0.08 percent of EDTA, 1.0-5.0 percent of glycerol, 0.01-0.5 percent of a preservative and the balance of water, wherein the traditional Chinese medicine extract is prepared from the following drug substances in parts by weight: 2-6 parts of flos lonicerae, 1-4 parts of radix salviae miltiorrhizae, 1-4 parts of radix codonopsitis, 1-3 parts of astragalus, 1-2 parts of radix ophiopogonis and 1-2 parts of cactaceae. Experiments show that the radiation-proof external skin-care composition is safe and non-irritating to skin, has a very good protective function in inhibiting skin fibroblastic proliferation caused by electron radiation, can effectively remove skin free radicals, and also has the bacteriostasis and skin barrier repair effects. The raw materials are easily accessible, the preparation method is simple and convenient, and the radiation-proof external skin-care composition is green and safe, can be easily accepted by allergic people and has a very good application prospect and market prospect.
Description
Technical field
The present invention relates to a kind of external medicine composition, specifically a kind of external medicine composition with radioprotective and reparation skin barrier effect and the skin care formulation prepared by this skin care compositions.
Background technology
The mankind are ingredients for ecosystem.People are survived by Environmental change condition, make the life better; Meanwhile, the change of environment also affects the clothing, food, lodging and transportion--basic necessities of life of the mankind and the health of the mankind.The multiple factor affecting skin health is there is, as ultraviolet, electrical equipment radiation, haze, acid rain, Enviromental pollutants etc. that we are familiar with in the external environment condition of our life.In recent years along with the raising of people's living standard, people's environmental pollution recognizing dangers improves constantly.In order to protect skin to encroach on from the external world, research environment contamination hazard and protection thereof are becoming one of focus of health care there research.
The skin protection grade of infringement type is defendd to pursue by consumer in existing market.The research and development of defence infringement type skin care item have an optimistic view of by many industry specialists.Major product effect that consumer pays close attention to has haze (preventing air contamination), radioprotective, anti-stimulation etc.Market is defendd the defence principle of infringement type skin care item main still to be isolated into master, auxiliary with effect of releiving.Protection approaches or single (as sunscreen product) or unilateral (as irritation product), or lack successes achieved in research support.Also do not have comprehensive defence infringement product, consumer can only use a product after, use a in addition, add consumer use complexity.
In addition, along with the covering that is universal gradually and mobile telephone base station of mobile phone is more and more intensive, people are in the radiation field of mobile phone constantly.Although the photon energy of mobile phone, lower than minimum energy ionizing radiation 1,000 ten thousand times, also can cause the threat of far-field radiation.And skin is now one of exposed area maximum contact time the longest organ, therefore, the impact of cell phone microwave radiation on skin molds structure also can not be ignored.Inventor finds, people are after long-time use mobile phone, and cellular radiation can cause the Proliferation Ability of skin flbroblast, and on skin, pathogenic microorganism increases, thus destroys skin barrier, causes skin aging.This kind of damage that skin-protection product commercially available at present all can not effectively suppress mobile phone and other electromagnetic radiation to cause skin.Therefore caused fibroblast proliferation by scavenging free radicals, suppression radiation, suppressed skin sex pheromone, maintenance skin barrier effect approach, reaching comprehensive environmental pollution of resisting is the emphasis that the present invention studies to the damage of skin.
Summary of the invention
Primary and foremost purpose of the present invention is to propose a kind of external skin-care composition having radioprotective and repair skin barrier effect.
Another object of the present invention is the preparation method proposing a kind of above-mentioned skin care compositions and the facial treatment repair obtained.
Invention thinking of the present invention is:
The present invention utilizes advanced biotechnology, in conjunction with the theory of Chinese medical science of Chinese tradition, Flos Lonicerae, Radix Salviae Miltiorrhizae, Radix Codonopsis, the Radix Astragali, Radix Ophiopogonis and Radix et Caulis Opuntiae Dillenii are effectively combined, form compound recipe, and it can be used as active component, be prepared into skin care formulation, it has protection radiation and causes fibroblast proliferation suppression, suppresses skin sex pheromone and maintenance skin barrier effect, thus effectively can prevent and improve skin aging.The present invention uses crude drug brief introduction as follows:
Flos Lonicerae: cold in nature, sweet in the mouth, enters lung, the heart, stomach warp, has heat-clearing and toxic substances removing, antiinflammatory, tonify deficiency treat effect of wind, cures mainly disease under distension, epidemic febrile disease heating, the diseases such as pyretic toxicity carbuncle and ulcer and tumor.
Radix Salviae Miltiorrhizae: nature and flavor are bitter, are slightly cold.GUIXIN, Liver Channel.There is stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, the effect of the relieving restlessness that clears away heart-fire.For menoxenia, amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia; Hepatosplenomegaly, angina pectoris.
Radix Codonopsis: sweet in the mouth, property is put down.Have invigorating the spleen and replenishing QI, quench the thirst, spleen invigorating lung benefiting, nourishing blood to promote the production of body fluid effect.For deficiency of both the splenic and pulmonary QI, lack of appetite asthenia, cough dyspnea due to deficiency, insufficiency of vital energy and blood, shallow complexion, shortness of breath and palpitation, Tianjin wound is thirsty, and interior-heat is quenched one's thirst.
The Radix Astragali: nature and flavor: sweet, tepor.Return lung, spleen, liver, kidney channel.Certain effect is had to exterior deficiency spontaneous perspiration, yang-energy weakness, skin infection diabrosis and syndrome of deficiency of lung qi.
Radix Ophiopogonis: nature and flavor are sweet, micro-hardship, is slightly cold.GUIXIN, lung, stomach warp.Function with cure mainly: YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire.For dryness of the lung dry cough, deficiency of YIN consumptive disease is coughed, sore throat pharyngalgia, and Tianjin wound is thirsty, and interior-heat is quenched one's thirst, vexed insomnia, dryness of the intestine constipation.
Radix et Caulis Opuntiae Dillenii: property mildly bitter flavor is cool.GUIXIN, lung, stomach three warp.Have heat-clearing and toxic substances removing, relaxing muscles and tendons and activating QI and blood in the collateral, dissipating blood stasis for subsidence of swelling, intestinal detoxication, cooling blood and relieving pain, intestine moistening is stopped blooding, effect of benefiting stomach and stopping pain and antitussive.
For realizing object of the present invention, the present invention adopts following concrete technical scheme:
Have the external skin-care composition of radioprotective and reparation skin barrier effect, wherein, described compositions is made up of the raw material of following weight percentage ratio:
Described Chinese medicine extract is made up of the crude drug of following weight parts proportioning:
Flos Lonicerae 2 ~ 6, Radix Salviae Miltiorrhizae 1 ~ 4, Radix Codonopsis 1 ~ 4, the Radix Astragali 1 ~ 3, Radix Ophiopogonis 1 ~ 2 and Radix et Caulis Opuntiae Dillenii 1 ~ 2.
The preparation method of described Chinese medicine extract is:
(1) take by described weight after broken for above-mentioned raw materials medicated powder, mixing;
(2) percent by volume is 60% ~ 90% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:8 ~ 1:20, and 70 DEG C ~ 90 DEG C are extracted 1 ~ 4 hour;
(3) extracting solution that step (2) obtains is cooled to 20 DEG C ~ 30 DEG C, 200 ~ 400 orders filter, and carry out vacuum filtration after leaching medicinal residues, collect filtrate;
(4) whole for filtrate rotary evaporation ethanol is obtained concentrated solution, add 1,3 butylene glycol in concentrated solution, addition is 60% ~ 90% of step (3) gained amount of filtrate percent by volume; Evenly miscible, filter, get clear liquid, to obtain final product.
In described step (2), the percent by volume of ethanol is 70%, and crude drug and ethanol mass volume ratio g/ml are 1:15; Extraction time is 3 hours.
In described step (3), vacuum filtration condition for spread two layers of filter paper in buchner funnel, and filter paper therebetween 0.3 ~ 0.6cm kieselguhr carries out vacuum filtration.
A preparation method for above-mentioned skin care compositions, wherein, described method step is as follows:
(1) EDTA glycerol is joined in deionized water, be stirred to after being heated to 80 DEG C ~ 90 DEG C and dissolve completely;
(2) add AVC, stir, make it dissolve completely;
(3) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extract, stir, to obtain final product.
Another kind has radioprotective and repairs the external skin-care composition of skin barrier effect, and wherein, described compositions is made up of the raw material of following weight percentage ratio:
Described Chinese medicine extract is made up of the crude drug of following weight parts proportioning:
Flos Lonicerae 2 ~ 6, Radix Salviae Miltiorrhizae 1 ~ 4, Radix Codonopsis 1 ~ 4, the Radix Astragali 1 ~ 3, Radix Ophiopogonis 1 ~ 2 and Radix et Caulis Opuntiae Dillenii 1 ~ 2.
The preparation method of described Chinese medicine extract is with above-mentioned.
A preparation method for above-mentioned skin care compositions, wherein, described method step is as follows:
(1) with water first by water solublity Jojoba oil heating for dissolving;
(2) EDTA, oat polypeptide, hyaluronate sodium, glycerol, allantoin, Aloe powder, stimulation inhibitory factor are joined in deionized water, be stirred to after being heated to 80 DEG C ~ 90 DEG C and dissolve completely;
(3) add AVC, stir, make it dissolve completely;
(4) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extract, stir, to obtain final product.
The skin care formulation obtained is facial treatment repair.Utilize Chinese medicine extract of the present invention and skin care field conventional method and adjuvant also can prepare other dosage forms, as cream frost, cosmetic water, spray etc.The preparation method of Chinese medicine composition of the present invention is not limited to this description and records, and field of Chinese medicines customary preparation methods all can play described effect.The method of the invention is best.
The recommendation method of preparation of the present invention is: after clean of every day, essence is coated on skin, massages gently, treat that essence slowly absorbs.
Beneficial outcomes of the present invention:
The present invention confirms non-stimulated to skin safe through experiment, has good protective effect to the skin flbroblast Proliferation Ability that electron radiation causes, and all right effective removing skin free radical, has antibacterial concurrently simultaneously and skin barrier repairs effect.Raw material of the present invention obtains easily, preparation method is easy, and green safety, is easy to be accepted by allergic human population, has good application prospect and market prospect.
Accompanying drawing explanation
Fig. 1 is the DPPH clearance rate figure of Chinese medicine composition A, B, C;
Fig. 2 is the percentage of water loss percentage ratio curve over time of skin surface.
Detailed description of the invention
The present embodiment medical material used all can be bought from China Medicament Group Corp. and obtain, and meets Pharmacopoeia of People's Republic of China 2005 editions standards.Other raw materials all obtain by commercially available purchase, and the present invention uses the source of raw material in table 1, and the instrument title that the present invention is used and producer are in table 2.
Table 1
Table 2
| Title | Model | Producer |
| Thermostat water bath | HH·S1-M | Chang'an, Beijing scientific instrument factory |
| High-speed multifunctional pulverizer | JP-400B-8 | Yongkang City nine grades of rank in the feudal regimes Trade Co., Ltd. |
| Intelligent temperature control heating stirrer | SZCL | Yu Hua Instrument Ltd. of Gongyi City |
| Rotary evaporator | RE-2000 | Shanghai Yarong Biochemical Instrument Plant |
| Circulating water type vacuum pump | SHB-Ⅲ | Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd. |
The preparation of embodiment 1 Chinese medicine composition of the present invention
(1) take by described weight after pulverizing according to following raw material medicaments, mixing;
Flos Lonicerae 40g, Radix Salviae Miltiorrhizae 20g, Radix Codonopsis 20g, Radix Astragali 20g, Radix Ophiopogonis 10g and Radix et Caulis Opuntiae Dillenii 10g.
(2) percent by volume is 70% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:15, are positioned in electric-heated thermostatic water bath, and 80 DEG C are extracted 3 hours;
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, leave standstill and be cooled to room temperature (20 DEG C), 200 order coarse filtrations, leach medicinal residues, carry out vacuum filtration again, sucking filtration condition: spread two layers of filter paper in buchner funnel, filter paper therebetween 0.5cm kieselguhr vacuum filtration, collects filtrate;
(4) in vacuum 0.1, under 43 DEG C of conditions, the whole ethanol of rotary evaporation obtains concentrated solution; Add 1,3 butylene glycol in concentrated solution, addition is 70% of step (3) gained amount of filtrate percent by volume, evenly miscible.Vacuum filtration, sucking filtration conditional synchronization rapid (3), gets clear liquid, to obtain final product.
The preparation of embodiment 2 Chinese medicine composition of the present invention
(1) take by described weight after pulverizing according to following raw material medicaments, mixing;
Flos Lonicerae 60g, Radix Salviae Miltiorrhizae 10g, Radix Codonopsis 10g, Radix Astragali 10g, Radix Ophiopogonis 20g and Radix et Caulis Opuntiae Dillenii 10g.
(2) percent by volume is 90% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:8, are positioned in electric-heated thermostatic water bath, and 70 DEG C are extracted 4 hours;
(3) taken out from thermostat water bath by the extracting solution that step (2) obtains, leave standstill and be cooled to room temperature, 200 order coarse filtrations, leach medicinal residues, obtain filtrate; This filtrate is carried out vacuum filtration again, sucking filtration condition: spread two layers of filter paper in buchner funnel, filter paper therebetween 0.3cm kieselguhr vacuum filtration, collect filtrate;
(4) in vacuum 0.1, under 43 DEG C of conditions, the whole ethanol of rotary evaporation obtains concentrated solution; Add 1,3 butylene glycol in concentrated solution, addition is 90% of step (3) gained amount of filtrate percent by volume, evenly miscible.Vacuum filtration, sucking filtration conditional synchronization rapid (3), gets clear liquid, to obtain final product.
The preparation of embodiment 3 Chinese medicine composition of the present invention
(1) take by described weight after pulverizing according to following raw material medicaments, mixing;
Flos Lonicerae 20g, Radix Salviae Miltiorrhizae 40g, Radix Codonopsis 40g, Radix Astragali 30g, Radix Ophiopogonis 10g and Radix et Caulis Opuntiae Dillenii 20g.
(2) percent by volume is 60% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:20, are positioned in electric-heated thermostatic water bath, and 90 DEG C are extracted 1 hour;
(3) taken out from thermostat water bath by the extracting solution that step (2) obtains, leave standstill and be cooled to room temperature, 100 order coarse filtrations, leach medicinal residues, obtain filtrate; This filtrate is carried out vacuum filtration again, sucking filtration condition: spread two layers of filter paper in buchner funnel, filter paper therebetween 0.6cm kieselguhr vacuum filtration, collect filtrate;
(4) in vacuum 0.1, under 43 DEG C of conditions, the whole ethanol of rotary evaporation obtains concentrated solution; Add 1,3 butylene glycol in concentrated solution, addition is 60% of step (3) gained amount of filtrate percent by volume, evenly miscible.Vacuum filtration, sucking filtration conditional synchronization rapid (3), gets clear liquid, to obtain final product.
The preparation of embodiment 4 essence of the present invention
Raw material and proportioning (mass percent), in table 3.
Table 3
| Material name | Consumption (wt%) |
| Embodiment 1 prepares Chinese medicine extract | 5.0 |
| AVC | 2.0 |
| EDTA | 0.01 |
| Glycerol | 5.0 |
| Antiseptic | 0.10 |
| Deionized water | Surplus |
Preparation method:
(1) EDTA glycerol is joined in deionized water, be stirred to after being heated to 80 DEG C and dissolve completely;
(2) add AVC, stir, make it dissolve completely;
(3) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extraction liquid, stir, to obtain final product.
The preparation of embodiment 5 essence of the present invention
Raw material and proportioning (mass percent), in table 4.
Table 4
| Material name | Consumption (wt%) |
| Embodiment 2 prepares Chinese medicine extract | 2.0 |
| AVC | 1.0 |
| EDTA | 0.08 |
| Glycerol | 1.0 |
| Antiseptic | 0.01 |
| Deionized water | Surplus |
Preparation method:
(1) EDTA glycerol is joined in deionized water, be stirred to after being heated to 90 DEG C and dissolve completely;
(2) add AVC, stir, make it dissolve completely;
(3) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extraction liquid, stir, to obtain final product.
The preparation of embodiment 6 essence of the present invention
Raw material and proportioning (mass percent), in table 5.
Table 5
| Material name | Consumption (wt%) |
| Embodiment 3 prepares Chinese medicine extract | 3.0 |
| AVC | 0.2 |
| EDTA | 0.05 |
| Glycerol | 3.0 |
| Antiseptic | 0.5 |
| Deionized water | Surplus |
Preparation method:
(1) EDTA glycerol is joined in deionized water, be stirred to after being heated to 85 DEG C and dissolve completely;
(2) add AVC, stir, make it dissolve completely;
(3) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extraction liquid, stir, to obtain final product.
The preparation of embodiment 7 essence of the present invention
Raw material and proportioning (mass percent), in table 6.
Table 6
| Material name | Consumption (wt%) |
| Embodiment 1 prepares Chinese medicine extract | 5.0 |
| AVC | 2.0 |
| EDTA | 0.01 |
| Oat polypeptide | 7.0 |
| Water solublity Jojoba oil | 1.0 |
| Hyaluronate sodium | 0.01 |
| Glycerol | 5.0 |
| Allantoin | 0.1 |
| Aloe powder | 0.5 |
| Stimulation inhibitory factor | 5.0 |
| Antiseptic | 0.10 |
| Deionized water | Surplus |
Preparation method:
(1) with deionized water first by water solublity Jojoba oil heating for dissolving;
(2) EDTA, oat polypeptide, hyaluronate sodium, glycerol, allantoin, Aloe powder, stimulation inhibitory factor are joined in deionized water, stirring and dissolving after being heated to 80 DEG C.
(3) add AVC, stir, make it dissolve completely.
(4) continue to be cooled to less than 40 DEG C, add antiseptic and Chinese medicine extract, stir, to obtain final product.
The preparation of embodiment 8 essence of the present invention
Raw material and proportioning (mass percent), in table 7.
Table 7
| Material name | Consumption (wt%) |
| Embodiment 2 prepares Chinese medicine extract | 2.0 |
| AVC | 0.2 |
| EDTA | 0.08 |
| Oat polypeptide | 1.0 |
| Water solublity Jojoba oil | 0.2 |
| Hyaluronate sodium | 0.05 |
| Glycerol | 1.0 |
| Allantoin | 0.2 |
| Aloe powder | 0.1 |
| Stimulation inhibitory factor | 1.0 |
| Antiseptic | 0.01 |
| Deionized water | Surplus |
Preparation method:
(1) with deionized water first by water solublity Jojoba oil heating for dissolving;
(2) EDTA, oat polypeptide, hyaluronate sodium, glycerol, allantoin, Aloe powder, stimulation inhibitory factor are joined in deionized water, be stirred to after being heated to 80 DEG C and dissolve completely;
(3) add AVC, stir, make it dissolve completely;
(4) continue to be cooled to less than 40 DEG C, add antiseptic and Chinese medicine extract, stir, to obtain final product.
The preparation of embodiment 9 essence of the present invention
Raw material and proportioning (mass percent), in table 8.
Table 8
| Material name | Consumption (wt%) |
| Embodiment 3 prepares Chinese medicine extract | 4.0 |
| AVC | 1.0 |
| EDTA | 0.03 |
| Oat polypeptide | 5.0 |
| Water solublity Jojoba oil | 0.5 |
| Hyaluronate sodium | 0.03 |
| Glycerol | 3.0 |
| Allantoin | 0.15 |
| Aloe powder | 0.3 |
| Stimulation inhibitory factor | 3.0 |
| Antiseptic | 0.5 |
| Deionized water | Surplus |
Preparation method:
(1) with deionized water first by water solublity Jojoba oil heating for dissolving;
(2) EDTA, oat polypeptide, hyaluronate sodium, glycerol, allantoin, Aloe powder, stimulation inhibitory factor are joined in deionized water, be stirred to after being heated to 80 DEG C and dissolve completely;
(3) add AVC, stir, make it dissolve completely;
(4) continue to be cooled to less than 40 DEG C, add antiseptic and Chinese medicine extract, stir, to obtain final product.
Efficacy experiments of the present invention
One, the anti-radiation effect evaluation of embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine extract A, B, C
(1) research purpose
By observing the impact that Chinese medicine extract A, B, C (being prepared by embodiment 1, embodiment 2 and embodiment 3 respectively) grows Mouse Skin Fibroblasts strain 3T6 after cellular radiation, evaluate Chinese medicine composition A, B, C to the cytostatic protective effect of cellular radiation.
(2) experiment material
By test product: embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine extract A, B, C
Cell strain: Mouse Skin Fibroblasts strain 3T6, purchased from Chinese Academy of Sciences's cell bank, uses after this laboratory passage.
1428479), 0.25%Trypsin-EDTA (lot number: 1213981) purchased from GIBCO company reagent: FBS (lot number:.Modified form RPMI-1640 type culture medium (lot number: NXL0745) is purchased from HYCLONE company.DMSO (lot number: 20121102) purchased from Chemical Reagent Co., Ltd., Sinopharm Group.Cell proliferation-toxicity detection-CCK-8 test kit is Japanese colleague's DOJINDO chemistry institute product.
Experimental apparatus: X71 type inverted microscope is Olympus Products.HHCP-01W type carbon dioxide cell incubator is Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd. product.The single one side clean work station of SW-CJ-IFD type is Purifying Equipment Co., Ltd., Suzhou's product.RT-6000 type microplate reader, Shenzhen thunder Du Products.Nokia's mobile phone 1050 type, frequency range is GSM900MHz.
(3) experimental technique
Experiment is divided into blank group, radiation matched group, medicine variable concentrations group, often organizes 8 multiple holes.Collect logarithmic (log) phase cell, counting, by 1 × 10
4/ ml concentration is inoculated into 96 orifice plates, every hole 200 μ l.Put 37 DEG C, 5%CO
2cultivate 24 hours in cell culture incubator, make cell attachment.Medication group adds different Chinese medicine extract 100 μ l, and blank group and radiation matched group add 100 μ l serum-free mediums, continues to cultivate.After 24 hours, cell plates are placed in CO
2other cell plates, except blank group extracellular, are placed on above mobile phone by incubator exoadaptation 1 hour, and mobile phone keeps talking state, and blank group cell plates are placed in above mobile phone in another room, and mobile phone keeps closed condition.Use CCK method to detect cytoactive after 6 hours, and calculate proliferation inhibition rate and Proliferation Ability protective rate.
(4) experimental result
Result shows, use cellular radiation after 6 hours, skin flbroblast propagation is suppressed, and absorbance is reduced, radiation cellular control unit OD value comparatively blank group compares and has significant difference (P<0.01), and proliferation inhibition rate reaches 16.89%; Chinese medicine extract A, B, C dosage is at 1000 μ gml
-1shi Jun has significant protective effect to cell inhibitory effect after radiation, can effectively reduce radiation to fibroblastic inhibitory action, thus absorbance rises, and compares have significant difference (P<0.05) with radiation matched group.In table 9, Chinese medicine extract A suppresses protective rate to reach 11.86% to fibroblast proliferation.
Table 9 Chinese medicine extract A, B, C are on the impact of cell proliferation/toxicity after radiation
| Group | Concentration μ gml -1 | Absorbance | Proliferation inhibition rate (%) | Proliferation Ability protective rate (%) |
| Chinese medicine extract A | 1000 | 1.18±0.09* | -- | 11.86 |
| Chinese medicine extract B | 1000 | 1.15±0.07* | -- | 9.57 |
| Chinese medicine extract C | 1000 | 1.13±0.05* | -- | 7.96 |
| Radiation matched group | -- | 1.04±0.09## | 16.89 | -- |
| Blank group | -- | 1.26±0.05 | -- | -- |
Two, embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine extract A, B, C are to protection effect of radiation indirect action
The effect of radiation to bioactive substance has two approach: one is directly act on biomacromolecule as DNA, and by ionizing and excite the fracture making it that chemical bond occur, cause the change of molecular structure and bioactive forfeiture, this effect is called direct effect; Two is that ray and hydrone interact, and produce the activation products such as free radical, the latter remakes for biomolecule, causes it to damage, and this type of effect is called indirect action.In vivo, due to a large amount of existence of hydrone, indirect action has prior meaning.Therefore, for radiation indirect action, scavenging free radicals effect can be selected to evaluate Chinese medicine extract A, B, C protection effect to radiation indirect action.
Embodiment 1 ~ 3 gained Chinese medicine extract and deionized water are mixed with the mass ratio of 3:97, obtains the aqueous solution of Chinese medicine extract; Using the aqueous solution of this Chinese medicine extract as test group; Wherein, embodiment 1 gained Chinese medicine extract A counter sample 1; Embodiment 2 gained Chinese medicine extract B counter sample 2; Embodiment 3 gained Chinese medicine extract C counter sample 3; Chinese medicine extract in test group is replaced with vitamin C derivatives, and obtained aqueous solution is as positive control.
Measurement result is as Fig. 1.Experimental result shows, when the mass percentage of each embodiment gained Chinese medicine extract in its aqueous solution is 3.0%, all have obviously DPPH Scavenging activity, there is the valid density reducing hydroxy radical, alkane free radical or oxyradical and the effect interrupting lipid peroxidation chain reaction, thus there is the effect of good delaying skin radiative aging.
Three, the bacteria resistance function evaluation of embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine extract A, B, C
The air pollutions such as haze, make the content of microorganisms in environment increase, and wherein common escherichia coli, staphylococcus aureus, propionibacterium acnes contact skin easily cause the phenomenon such as skin acne, sensitivity.
Embodiment 1 ~ 3 gained Chinese medicine extract A, B, C are carried out escherichia coli, staphylococcus aureus, propionibacterium acnes suppression efficacy assessments.
Odontothrips loti: configure corresponding culture medium according to different strains tested, high pressure steam sterilization 121 DEG C, 20min.Making solid medium is dull and stereotyped, 20mL culture medium about poured into by each culture dish, after culture medium solidifying, getting 100 μ L bacterium liquid (through the bacteria suspension of Maxwell turbidimetry for Determination concentration) with liquid-transfering gun instills in culture dish, adopt spread plate, bacteria suspension is spread evenly across on solid medium.Directly vertically put Oxford cup (the circular tubule of internal diameter 6mm, external diameter 8mm, high 10mm) in media surface, pressurize gently, make it contact tight with culture medium.Three Oxford cups placed by each culture dish.Add 200 μ L testing samples in the cup of each Oxford, the culture dish of antibacterial is gently placed in bacteriological incubator 37 DEG C and cultivates 16 hours.
Wherein, embodiment 1 gained Chinese medicine extract A counter sample 1; Embodiment 2 gained Chinese medicine extract B counter sample 2; Embodiment 3 gained Chinese medicine extract C counter sample 3; Positive control is 0.2%MTI (Methylisothiazolinone/iodine propinyl butyl carbamate).MTI is antiseptic conventional in cosmetics, and suggestion consumption is 0.05% ~ 0.02%.Excellent antibacterial activity is all had to antibacterial, fungus.Measurement result is as table 10.
Table 10 Chinese medicine extract A, B, C fungistatic effect is evaluated
| Antibacterial circle diameter (mm) | Escherichia coli | Staphylococcus aureus | Propionibacterium acnes |
| Sample 1 | 24.95±0.22 | 14.71±0.18 | 16.94±0.42 |
| Sample 2 | 20.36±0.46 | 16.09±0.17 | 14.27±0.11 |
| Sample 3 | 19.90±0.57 | 13.04±0.39 | 14.65±0.78 |
| Positive control | 21.23±0.64 | 17.92±0.37 | 20.90±0.98 |
Note: antibacterial circle diameter >=20mm has been judged to bacteriostasis for extremely responsive " +++ ", and the larger fungistatic effect of inhibition zone is better; 15mm≤bacteriostatic diameter <20mm is Gao Min " ++ "; 10mm≤bacteriostatic diameter <15mm is in being quick "+"; Bacteriostatic diameter <10mm is invalid, is judged to without bacteriostasis.With reference to Chen Yuping. the susceptibility test methods [J] of antibacterials. animal and veterinary scientific and technological information, 2007, (5): 88-89.
As can be seen from experimental result, sample 1, sample 2, sample 3 all have good fungistatic effect, effectively can to reduce in environment microorganism to the adverse effect of skin.
Four, the anti-radiation effect evaluation of embodiment 7, embodiment 8, embodiment 9 gained preparation
By corresponding for embodiment 7 gained preparation essence 1; The corresponding essence 2 of embodiment 8 gained preparation; The corresponding essence 3 of embodiment 9 gained preparation.
Another using table 11 as negative control.Negative control essence preparation method used is as follows:
According to table 11 gained formula, with deionized water first by water solublity Jojoba oil heating for dissolving.Stirring and dissolving after EDTA, oat polypeptide, hyaluronate sodium, glycerol, allantoin, Aloe powder, stimulation inhibitory factor are joined in deionized water and be heated to 80 DEG C; Add AVC, stir, make it dissolve completely.Be cooled to less than 40 DEG C, add antiseptic, stir.
Table 11 negative control essence formula
| Material name | Consumption (wt%) |
| AVC | 2.0 |
| EDTA | 0.01 |
| Oat polypeptide | 7.0 |
| Water solublity Jojoba oil | 1.0 |
| Hyaluronate sodium | 1.0 |
| Glycerol | 5.0 |
| Allantoin | 0.1 |
| Aloe powder | 0.5 |
| Stimulation inhibitory factor | 5.0 |
| Deionized water | Surplus |
| Antiseptic | 0.10 |
Kunming kind hairless mouse, 60, male and female half and half, body weight 18 ~ 22g, is provided by Beijing Vital River Experimental Animals Technology Co., Ltd..
Experimental technique:
60 mices are divided into 6 groups at random, that is: 1. Normal group, 2. radiation matched group, 3. 1 group, sample, 4. 2 groups, sample, 5. 3 groups, sample, 6. negative control group.Often organize 10, by sex and group sub-cage rearing, under equivalent environment, control temperature maintains 20 ~ 25 DEG C, 12h illumination, and 12h is dark, and routine raises food drinking-water, without undesirable element impact, raises after within one week, adapting to laboratory environment and starts to test.
Mobile phone is placed on special in the cage of this experimental design (diameter 45cm, high 11cm).Nokia's mobile phone 1050 type (frequency range is GSM900MHz) is adopted to carry out radiation test as radiation source.
Be divided at random by 60 mices 6 groups (often organizing 10), do not smear sample for one group, experimental session mobile phone keeps closed condition, as Normal group.Do not smear sample for one group, experimental session mobile phone keep opening state, and in every day 10 time, 14 time, 16 time, be in on-state, connect 15min at every turn, as radiation matched group.Other four groups as experimental group, experimental session, mobile phone keep opening state, and in every day 10 time, 14 time, 16 time, be in on-state, connect 15min at every turn.Smear sample 1 respectively, sample 2, sample 3, negative control essence sample.Respectively sample is evenly embrocated in mouse back, be 0.5mL at every turn, once a day, smear evenly and mice is placed in the cage of radiation source, each 45min, once a day, continue 96 days.
96th day, lost hair or feathers by mouse back apparatus, area was 2cm × 3cm.After disconnected neck puts to death whole mice, take off back depilation place full thickness skin (not containing subcutaneous fat) immediately, be frozen in preservation in-70 DEG C of refrigerators to be measured.Adopt histopathologic slide's tabletting technology, HE dyes, and changes with 40 times of om observation dermis, and it is active to measure SOD with biochemical analysis method, MDA content.
SOD can remove super oxygen cation (i.e. oxygen-derived free radicals), and Cell protection is from infringement.SOD vigor height can reflect the ability of body scavenging activated oxygen indirectly.Free radical attacks the unsaturated fatty acid in film fat, and make it be oxidized, the catabolite MDA of lipid peroxide will raise, by measuring MDA content in body, and can the degree of injury of radical pair body in antimer indirectly.
All data are all processed by SPSS19.0 software, and each group experiment parameter represents with .x ± s.
Table 12 is respectively organized in hairless mouse skin tissue SOD, MDA content (x ± s) (n=10)
In mouse skin, SOD, MDA content is in table 12.Result shows: embodiment 7 (counter sample 1), embodiment 8 (counter sample 2) and embodiment 9 (counter sample 3) all have radioprotective effect.And the protection effect of embodiment 7 is the most remarkable.Inventor utilizes embodiment 4,5,6 gained essence to repeat above-mentioned experiment, and result display all has radioprotective effect.
Five, the maintenance barrier of embodiment 7 gained preparation is evaluated
1. horny layer moisture loss test
Test sample is essence, prepares all as follows and obtains:
By corresponding for embodiment 7 gained preparation essence 1.
Another using table 11 essence as blank.Blank essence preparation method used evaluate with above-mentioned anti-radiation effect in negative control preparation method identical.
The front health volunteer 30 (men and women each 15) trained through professional of test, age was at 20 ~ 30 years old, all experimenters are all without skin or systemic disease history, and recipient site is without exception, and tested period does not smear any medicine irrelevant with experiment or cosmetics.
Test site room temperature (25 ± 1) DEG C, relative humidity (40 ± 5) %, before test, experimenter should keep body to be steady statue.After using the clear water wiping experimenter both arms of about 35 DEG C, after the 30min that sits quietly under test environment, start test.
At experimenter's inboard arm apart from palm base portion 5cm place, the tested region of labelling (4cm × 4cm), same arm can with the multiple region of tense marker (interval 1cm), the equal random distribution of test sample.First measure the blank value of each test zone, then test specimens is by (2.0 ± 0.1) mg/cm
2consumption carry out single application, adopt Multi-functional skin moisture tester and the moisture of skin instrument that scatters and disappears to measure the moisture loss value in the tested region of 0.5h, 1h, 2h and 4h and blank region respectively after coating.
Experimenter is smeared the data that in same sample region, 5 times record and averages by test, adopts SPSS statistical software to carry out t check analysis.
Experimental result
Moisture of skin scatters and disappears and changes
Moisture of skin windage (Transepidermalwaterloss, TEWL) reflect in test period, Experimental Area moisture loss rule over time, it can the skin barrier maintenance function of characterization test sample, its value is less, moisture loss is fewer, and skin repair ability is stronger; Otherwise skin repair ability is more weak.
Percentage of water loss (%)=(sample sets water loss amount-starting moisture windage)/starting moisture windage × 100
Moisture loss the results are shown in Figure 2.Result shows: in 4h, embodiment 7 (counter sample 1) has skin repair ability effect.It is the same that inventor repeats above-mentioned experimental result with essence prepared by embodiment 4,5,6,8,9.And the maintenance most pronounced effects of embodiment 7.
Claims (10)
1. have the external skin-care composition of radioprotective and reparation skin barrier effect, it is characterized in that, described external skin-care composition is made up of the raw material of following weight percentage ratio:
Described Chinese medicine extract is made up of the crude drug of following weight parts proportioning:
Flos Lonicerae 2 ~ 6, Radix Salviae Miltiorrhizae 1 ~ 4, Radix Codonopsis 1 ~ 4, the Radix Astragali 1 ~ 3, Radix Ophiopogonis 1 ~ 2 and Radix et Caulis Opuntiae Dillenii 1 ~ 2.
2. external skin-care composition as claimed in claim 1, it is characterized in that, the preparation method of described Chinese medicine extract is:
(1) take by described weight after crude drug described in claim 1 being pulverized, mixing;
(2) percent by volume is 60% ~ 90% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:8 ~ 1:20, and 70 DEG C ~ 90 DEG C are extracted 1 ~ 4 hour;
(3) extracting solution that step (2) obtains is cooled to 20 DEG C ~ 30 DEG C, 200 ~ 400 orders filter, and carry out vacuum filtration after leaching medicinal residues, collect filtrate;
(4) whole for filtrate rotary evaporation ethanol is obtained concentrated solution, add 1,3 butylene glycol in concentrated solution, addition is 60% ~ 90% of step (3) gained amount of filtrate percent by volume; Evenly miscible, filter, get clear liquid, to obtain final product.
3. external skin-care composition as claimed in claim 2, is characterized in that, in described step (2), the percent by volume of ethanol is 70%, and crude drug and ethanol mass volume ratio g/ml are 1:15; Extraction time is 3 hours.
4. external skin-care composition as claimed in claim 2, is characterized in that, in described step (3), vacuum filtration condition for spread two layers of filter paper in buchner funnel, and filter paper therebetween 0.3 ~ 0.6cm kieselguhr carries out vacuum filtration.
5. the preparation method of external skin-care composition described in any one in Claims 1-4, it is characterized in that, described preparation method step is as follows:
(1) EDTA glycerol is joined in deionized water, be stirred to after being heated to 80 DEG C ~ 90 DEG C and dissolve completely;
(2) add AVC, stir, make it dissolve completely;
(3) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extract, stir, to obtain final product.
6. have the external skin-care composition of radioprotective and reparation skin barrier effect, it is characterized in that, described external skin-care composition is made up of the raw material of following weight percentage ratio:
Described Chinese medicine extract is made up of the crude drug of following weight parts proportioning:
Flos Lonicerae 2 ~ 6, Radix Salviae Miltiorrhizae 1 ~ 4, Radix Codonopsis 1 ~ 4, the Radix Astragali 1 ~ 3, Radix Ophiopogonis 1 ~ 2 and Radix et Caulis Opuntiae Dillenii 1 ~ 2.
7. external skin-care composition as claimed in claim 6, it is characterized in that, the preparation method of described Chinese medicine extract is:
(1) take by described weight after broken according to above-mentioned raw materials medicated powder, mixing;
(2) percent by volume is 60% ~ 90% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:8 ~ 1:20, and 70 DEG C ~ 90 DEG C are extracted 1 ~ 4 hour;
(3) extracting solution that step (2) obtains is cooled to 20 DEG C ~ 30 DEG C, 200 ~ 400 order coarse filtrations, after leaching medicinal residues, carries out vacuum filtration, collect filtrate;
(4) whole for filtrate rotary evaporation ethanol is obtained concentrated solution, add 1,3 butylene glycol in concentrated solution, addition is 60% ~ 90% of step (3) gained amount of filtrate percent by volume; Evenly miscible, filter, get clear liquid, to obtain final product.
8. external skin-care composition as claimed in claim 7, is characterized in that, in described step (2), the percent by volume of ethanol is 70%, and crude drug and ethanol mass volume ratio g/ml are 1:15; Extraction time is 3 hours.
9. external skin-care composition as claimed in claim 7, is characterized in that, in described step (3), vacuum filtration condition for spread two layers of filter paper in buchner funnel, and filter paper therebetween 0.3 ~ 0.6cm kieselguhr carries out vacuum filtration.
10. the preparation method of external skin-care composition described in any one in claim 6 to 9, it is characterized in that, described preparation method step is as follows:
(1) with water first by water solublity Jojoba oil heating for dissolving;
(2) EDTA, oat polypeptide, hyaluronate sodium, glycerol, allantoin, Aloe powder, stimulation inhibitory factor are joined in deionized water, be stirred to after being heated to 80 DEG C ~ 90 DEG C and dissolve completely;
(3) add AVC, stir, make it dissolve completely;
(4) be cooled to less than 40 DEG C, add antiseptic and described Chinese medicine extract, stir, to obtain final product.
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| CN103417414A (en) * | 2012-05-20 | 2013-12-04 | 李一凡 | Extracting method for cactus primary juice |
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| CN102068606A (en) * | 2010-11-26 | 2011-05-25 | 新时代健康产业（集团）有限公司 | Wrinkle-removing and anti-wrinkle Chinese medicinal composition, preparation and preparation method thereof |
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| WO2024002058A1 (en) * | 2022-06-27 | 2024-01-04 | Oneness Biotech Co., Ltd. | Plectranthus amboinicus extract for use in alleviation of radiation-induced skin disorders |
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