CN108852944A - It is a kind of using OPC as tridecanoic peptide antibacterial repair latex of carrier and preparation method thereof - Google Patents
It is a kind of using OPC as tridecanoic peptide antibacterial repair latex of carrier and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of using OPC as tridecanoic peptide antibacterial repair latex of carrier and preparation method thereof, including tridecanoic peptide, carbomer, glycerol, deionized water.Carbomer is high molecular polymer made of mountain acrylic acid and allyl saccharose crosslinking, is white loose, the extremely strong powder of hygroscopicity, water-soluble, ethyl alcohol and glycerol, can form transparent semi-solid substance after being swollen in water.With good emulsibility, thickening property, suspending and film forming.Carbomer and skin misfortune conjunction effect are good simultaneously, can form one layer of moist repair membrane in skin surface.Glycerol is the moisturizer of traditional classical, there is good water imbibition, can keep skin wet, softness, can form one layer of moisturizing film on the skin, and isolation foreign substance invades skin, prevents moisture from evaporating.To having the function of antibacterial and reparation after the sensitive skin such as dermatitis, eczema and cosmetology, and preservative is not contained in product of the present invention, being used for a long time has higher safety.
Description
Technical field
The present invention relates to cosmetic fields, and in particular to it is a kind of using OPC as the tridecanoic peptide antibacterial repair latex of carrier and its
Preparation method.
Background technique
After the sensitivity skin such as dermatitis, eczema and cosmetology, after solarization and physics, chemistry etc. stimulate caused by damaged skin,
The decline of skin moisture-keeping ability, skin physiology moisture are largely lost, and gap increases between epidermal cell, and environmental stimuli is easy for wearing
Transdermal barrier invades skin, further induces and causes scytitis by microorganism, extends the treatment time of skin disease, together
When over-drying surface of a wound environment, not only lead to pruitus, influence patients ' life quality, and scratching will lead to skin repeatedly
Skin inflammation recurrent exerbation.Have bacteriostasis lotion many kinds of currently on the market, majority is using the chemicals using complexity
Matter, cosmetic additive agent etc. reach skin care effect, carry out cosmetics anti-corrosion using chemical preservative, these ingredients are to skin
Stimulation is very big, frequently can lead to strong sensitization.
This patent main component is using procyanidine as the carbomer of carrier, tridecanoic peptide, glycerol, deionized water mixture group
At.
Tridecanoic peptide is a kind of multifunctional bio polypeptide obtained by Fermentation Engineering, and main function is antibacterial anti-inflammatory, promotes
Wound healing repairs skin barrier, improves skin and mucosa-immune power, to inhibiting skin bacterium growth, treatment acne, dermatitis,
The illnesss such as skin lesion reduce scar and are formed, repair skin barrier, prevent trauma surface infestation, and wound healing has remarkable effect.
The present invention is leaf of Fructus vaccini vitis-idaeae extract (OPC) using procyanidine, and product emulsion market is almost blank.Experiment card
Bright, the free radical resisting oxidability of OPC is 50 times of vitamin E, 20 times of vitamin C.It has following effect to skin：(1)
Increase elasticity：Skin belongs to connective tissue, and collagen and hard elastics albumen contained therein plays the total of skin
Important role.
OPC is used cooperatively with tridecanoic peptide：On the one hand it can promote collagen formed it is appropriately crosslinked；On the other hand, it makees
For a kind of effective free radical scavenger, the generation of this unusual physiological status of collagen " excessively crosslinking " can be prevented, thus
Also it is prevented that the appearance of wrinkle of skin and vesica, keeps the submissive smooth of skin.Hard elastics albumen can be by free radical or hard bullet
Property protease degraded, the cutis laxa for lacking hard elastics albumen is powerless, as the clothes of fold drapes over one's shoulders with.OPC can also be steady
Determine collagen and hard elastics albumen, fundamentally improves skin elasticity.(2) wrinkle is reduced：As human skin is by a large amount of
The infringement of free radical and the decline of antiradical activities, under the damage of extraneous ultraviolet light, human skin accelerates aging and rises
Wrinkle.OPC is a kind of beauty and skin care health-care good product that is efficient anti-oxidant and removing free radical.And it is easy to by skin connective tissue
It absorbs, it can assist to protect the skin from ultraviolet damage.OPC removes free radical, has blocked the generation of hard elastics protease,
And inhibit its activity, thus from the internal health status for improving skin.
Carbomer and skin good mixing effect can form one layer of moist repair membrane in skin surface.Glycerol is traditional warp
The moisturizer of allusion quotation has good water imbibition, can keep skin wet, softness, can form one layer of moisturizing film on the skin,
Completely cut off foreign substance and invade skin, prevents moisture from evaporating.It can be effectively improved patient skin drying, furfur phenomenon, promote inflammation
Subside.Damaged skin barrier is repaired, damage of the environmental stimuli to skin is reduced, plays anti-inflammatory, anti-allergic effects, while can be with
Pigmented generation is reduced, and does not contain preservative in product of the present invention, long-time service has higher safety.
Summary of the invention
Present invention aim to address decortication, erythema, pruritis caused by dermatitis, eczema, sensitive, are used for
Moisturizing restoration care after beauty, orthopedic, provides that a kind of using OPC to be that the tridecanoic peptide antibacterial repair latex of carrier has super
Strong antibacterial action simultaneously can form one layer of moist repair membrane in skin surface, can keep skin wet, softness, completely cut off external
Substance invades skin, has the effect of antibacterial and wound healing, and provide the preparation method of antibacterial repair latex, operates
Simply, easy to use.
It is a kind of using OPC as the tridecanoic peptide antibacterial repair latex of carrier, weight percentage, including following components：Card
Wave nurse 0.2%, procyanidine 0.4%-0.8%, polymer hyaluronic acid 0.1%, low molecular weight hyaluronic acid 0.1%, glycerol
3%, tridecanoic peptide 12%-24%, mineral oil 10%, self-emulsifying glycerin monostearate 2%, triethanolamine 0.2%, surplus are
Purified water；The tridecanoic peptide molecular formula is： Cys-Phe-Arg-Val-Cys-Tyr-Arg-Gly-Ile-Cys-Tyr-Arg-
Cys。
Preferably, weight percentage, including following components：Carbomer 0.2%, procyanidine 0.5%, height
Molecular weight hyaluronic acid 0.1%, low molecular weight hyaluronic acid 0.1%, glycerol 3%, tridecanoic peptide 15%, mineral oil 10%, self-emulsifying list
Tristerin 2%, triethanolamine 0.2%, surplus are purified water；The tridecanoic peptide molecular formula is： Cys-Phe-Arg-
Val-Cys-Tyr-Arg-Gly-Ile-Cys-Tyr-Arg-Cys。
Preferably, the procyanidine is leaf of Fructus vaccini vitis-idaeae extract (OPC).
Preferably, the procyanidin content is greater than 95%.
Preferably, the preparation method of antibacterial repair latex, includes the following steps：
Step 1：Each component content is calculated, the purified water of corresponding amount is added in water phase container；
Step 2：Carbomer, polymer hyaluronic acid, low molecular weight hyaluronic acid, stirring are sequentially added into water phase container
Homogeneous is carried out using homogenizer after uniformly；
Step 3：OPC is added into water phase container, stirs, adds glycerol, stirs, then
Tridecanoic peptide is added, stirs, the liquid in water phase container is then heated to 70 DEG C, heat preservation；
Step 4：Mineral oil is added into oily phase container, self-emulsifying glycerin monostearate is then added, oil is compatible
Liquid in device is heated to 75 DEG C, melts self-emulsifying glycerin monostearate, then stirs evenly, and keeps the temperature；
Step 5：Liquid in oily phase container is poured into water phase container, stirs while adding, stirs,
Form uniform lotion；
Step 6：The triethanolamine being slowly added into above-mentioned emulsion, stirs while adding, and forms the equal of viscosity qualification
Then even lotion cools to room temperature；
Step 7：Sterilizing, it is filling after quality inspection is qualified.
Preferably, a kind of using OPC as the preparation method of the tridecanoic peptide antibacterial repair latex of carrier, in the step 5
It is emulsified using emulsifier.
Preferably, after the antibacterial repair latex is mainly used for the sensitive skin such as dermatitis, eczema and cosmetology, solarization
Afterwards and physics, chemistry etc. stimulate caused by damaged skin.
The beneficial effects of the present invention are embodied in following aspect：
The present invention provides a kind of using OPC as tridecanoic peptide antibacterial repair latex of carrier and preparation method thereof, main component packet
Include tridecanoic peptide, carbomer, glycerol, deionized water.Tridecanoic peptide is a kind of multifunctional bio polypeptide obtained by Fermentation Engineering,
Main function is antibacterial anti-inflammatory, wound healing, repairs skin barrier, improves skin and mucosa-immune power, to inhibition skin
Bacterial growth.Carbomer is high molecular polymer made of mountain acrylic acid and allyl saccharose crosslinking, is white loose, moisture absorption
Property extremely strong powder, water-soluble, ethyl alcohol and glycerol can form transparent semi-solid substance after being swollen in water.Have
Good emulsibility, thickening property, suspending and film forming.Carbomer and skin misfortune conjunction effect are good simultaneously, can be in skin surface
Form one layer of moist repair membrane.Glycerol is the moisturizer of traditional classical, there is good water imbibition, can keep skin wet, soft
It is soft, one layer of moisturizing film can be formed on the skin, and isolation foreign substance invades skin, prevents moisture from evaporating, the present invention relates to each
Kind of component comprehensive function is to after the sensitive skin such as dermatitis, eczema and cosmetology, after solarization and caused by the stimulation such as physics, chemistry
Damaged skin has the function of antibacterial and reparation, and preservative is not contained in product of the present invention, and long-time service has higher peace
Quan Xing.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail：
1, Examples 1 to 5
The Examples 1 to 5 component table of 1 antibacterial repair latex of table
Above-mentioned tridecanoic peptide molecular formula is： Cys-Phe-Arg-Val-Cys-Tyr-Arg-Gly-Ile-Cys-Tyr-Arg-
Cys。
It is leaf of Fructus vaccini vitis-idaeae extract (OPC) that above-mentioned procyanidine, which is procyanidine, and procyanidin content
Greater than 95%, remaining component is commercially available.
Emulsion preparation method involved by above-mentioned 1~5 embodiment is：
Step 1：Each component content is calculated, the purified water of corresponding amount is added in water phase container；
Step 2：Carbomer, polymer hyaluronic acid, low molecular weight hyaluronic acid, stirring are sequentially added into water phase container
Homogeneous is carried out using homogenizer after uniformly；
Step 3：OPC is added into water phase container, stirs, adds glycerol, stirs, then
Tridecanoic peptide is added, stirs, the liquid in water phase container is then heated to 70 DEG C, heat preservation；
Step 4：Mineral oil is added into oily phase container, self-emulsifying glycerin monostearate is then added, oil is compatible
Liquid in device is heated to 75 DEG C, melts self-emulsifying glycerin monostearate, then stirs evenly, and keeps the temperature；
Step 5：Liquid in oily phase container is poured into water phase container, stirs while adding, stirs,
Form uniform lotion；
Step 6：The triethanolamine being slowly added into above-mentioned emulsion, stirs while adding, and forms the equal of viscosity qualification
Then even lotion cools to room temperature；
Step 7：Sterilizing, it is filling after quality inspection is qualified.
The step 5 is emulsified using emulsifier.
2, experimental example 1
Measure Examples 1 to 5 inhibitory effect involved in the present invention.
For trying bacterium
2.1 bacterium：Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922
Saccharomycete：Candida albicans (ATCC14053).
The preparation of 2.2 bacterium solutions：The nutrient agar inclined-plane fresh cultured object (18~for 24 hours) in the 3rd~14 generation of bacterial strain is taken,
Lower lawn is washed with 5mL0.03mol/L phosphate buffer (hereinafter referred to as PBS), is diluted after so that bacterium is suspended uniformly with above-mentioned PBS
To required concentration.
2.3. operating procedure
2.3.1 take 10 μ L bacterial strains in 1mL liquid nutrient media (bacterium) or sabouraud culture medium (saccharomycete), 37 DEG C
It is incubated overnight in shaking table.
2.3.2 the bacterial strain activated is diluted step by step with sterile PBS, take 50 μ L to be applied to nutrition fine jade after being diluted to debita spissitudo
Rouge culture medium (bacterium) or sabouraud's agar (saccharomycete) are incubated overnight in 37 DEG C of incubators.
2.3.3 dyeing microscopic examination, colonial morphology is correct, no miscellaneous bacteria.
2.3.4 bacterium amount is calculated according to applicability result, is about 108cfu/mL with sterile PBS adjustment bacterium amount.
2.3.5 it takes 1 μ L bacterium solution to be connected in 1mL test sample, mixes, room temperature stored protected from light, take 50 μ L to apply according to the regulation time
It is incubated overnight in agar medium, 37 DEG C of incubators.
2.4 experimental result
The Examples 1 to 5 of 2 antibacterial repair latex of table is to Escherichia coli, staphylococcus aureus, the antibacterial effect of Candida albicans
Power
Positive controls are that tridecanoic peptide is removed in embodiment 2, and 1% benzoic acid is added.
NR：The non-restoration ecosystem of test organisms
NI：Do not increase, refers to that, to previous minute, the increased quantity of test organisms is no more than 0.5lg
3, experimental example 2
Examples 1 to 5 Bactericidal test
For trying bacterium
3.1 bacterium：Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922)；Saccharomycete：White is read
Pearl bacterium (ATCC14053).
The preparation of 3.2 bacterium solutions：The nutrient agar inclined-plane fresh cultured object (18~for 24 hours) in the 3rd~14 generation of bacterial strain is taken,
Lower lawn is washed with 5mL0.03mol/L phosphate buffer (hereinafter referred to as PBS), is diluted after so that bacterium is suspended uniformly with above-mentioned PBS
To required concentration.
3.3. operating procedure
3.3.1 by test organisms, slant culture is washed down with PBS for 24 hours, be made bacteria suspension (it is required that concentration be：With 100 μ L
It drips in 30mLPBS liquid, bacterial count recovered is 1 × 104~9 × 104cfu/mL).
3.3.2 each sample liquid (5mL) and PBS control sample are taken.
3.3.3 sterile batch cultur plate is taken, it is (thin that sterilized nutrient agar is poured into each plate
Bacterium) or sabouraud's agar (saccharomycete) 20ml, it is cooling stand-by.
3.3.4 sterilized semisolid culturemedium is taken, specification is every test tube 10ml, heating water bath to thawing；To cold
But it to preference temperature, is added in test tube for examination 100 μ L of bacterium, is poured into after shaking up in the agar medium of cooled and solidified, it is cold
Stand-by.
3.3.5 it is punched on above-mentioned plate with punch.
3.3.6 it is separately added into 100 μ L of test sample in each hole, plate is placed in 35 DEG C of ± 2 DEG C of cultures in incubator
12h (bacterium) or 18h (saccharomycete), with vernier caliper measurement antibacterial circle diameter.
3.5 experimental result
The Examples 1 to 5 of 3 antibacterial repair latex of table is to staphylococcus aureus, Escherichia coli, Candida albicans inhibition zone
Measuring diameter
As shown in table 3, tridecanoic peptide content gradually increases in Examples 1 to 5, in staphylococcus aureus, Escherichia coli
And the antibacterial circle diameter in Candida albicans culture medium is gradually increased, and doses effect relation, the positive controls are presented
Constituent content is that tridecanoic peptide is removed in embodiment 2, and 1% benzoic acid is added, and positive controls biocidal property differs nothing with embodiment 2
A few combination economic effects consider that selection example 2 is used as most preferred embodiment.
4, experimental example 3
Selection example 2 carries out clinical trial.
Subject totally 30 of Pass Test condition are included in this test.Concrete condition is described as follows：
It is included in 30 altogether, wherein male 8, the age, average age was 30.5 years old from -48 years old 26 years old；Women 22, year
Age, average age was 31.4 years old from -50 years old 22 years old.Wherein, 2, drying sensitivity skin, various the dermatitis, (packet of eczema 16
It includes：Cosmetic allergic contact dermatitis, steroid dependent dermatitis, seborrhea, allergic dermatitis, acne, eczema), skin ulcer, pressure sore,
Burn and scald totally 7, beauty and shaping and beauty postoperative totally 5.Medical history is from 1 day to 3 year, and average medical history 6.5 months.
Clinical testing procedure：
4.1 inclusion criteria
4.11 voluntary participation；
4~60 one full year of life of 4.12 age, men and women are unlimited；
4.13 clinical diagnosises be dermatitis, eczema, after cosmetology, skin damage patient after shining；
4.14 skin lesions are in skin surface；
4.15 patients such as use other external drugs or oral drug therapy, need to deactivate 10 days or more persons；
4.2 exclusion criteria
4.2.1 there are serious systemic disease patient, including the heart, liver, kidney, metabolism and endocrine, hematological system, autoimmunity
Property disease and malignant tumour etc.；
4.2.2 known or suspicious to this product ingredient allergy sufferers；
4.2.3 the patient of other therapies must clinically be received.
Test method：Selection meets subject 30 of this experimental condition, repairs to the tridecanoic peptide antibacterial that OPC is carrier
Double emulsion (the Jilin Province land Lan Ding and Science and Technology Ltd.'s production), direct spraying is in skin lesion.1-3 times daily.Each 15-30
Minute
Used statistical method and evaluation method：
Statistical method：Using analysis of cases method, judge using OPC as the clinic of the tridecanoic peptide antibacterial repair latex of carrier
Use curative effect.
Evaluation method:
To using OPC as efficient for statistical analysis, the test group total effective rate of the tridecanoic peptide antibacterial repair latex of carrier
More than 85%, can assert effectively.
Evaluation criterion：
Improve the functional evaluation of inflammatory reaction：Press symptom：Itch.Sign：Erythema, the scales of skin that peel off.Standards of grading：By 0~3
Divide meter.Therapeutic index=(pre-treatment score-post treatment integral)/pre-treatment score × 100%.Complete incidence graph is therapeutic index
>=90%；Significant alleviate is therapeutic index >=50%；Minor responses are therapeutic index >=20%；It is in vain therapeutic index <
20%.Effective percentage is calculated with complete incidence graph+significant alleviate.
Clinical test results：
Symptom of erythema improves：
Furfur symptom improves：
Pruritis improves：
The adverse events and side effect and its disposition found in clinical test：
Adverse events and side effect are not found during entire clinical test.
Clinical trial result analysis：
After the sensitive skin such as the case majority that this clinical test selects is dermatitis, eczema and cosmetology, after solarization and object
Damaged skin caused by reason, chemistry etc. stimulate, the decline of skin moisture-keeping ability, skin physiology moisture is largely lost, between epidermal cell
Gap increases, and environmental stimuli is easy for penetrating skin barrier intrusion skin, further induces scytitis.Extend skin disease
The treatment time of disease, while over-drying surface of a wound environment, not only lead to pruitus, influences patients ' life quality, and anti-
Multiple scratching will lead to scytitis recurrent exerbation.The product main component of this test is using OPC as the carbomer of carrier, amino
Acid polymer, glycerol, deionized water mixture composition.Carbomer and skin misfortune conjunction effect are good, can form one in skin surface
The moist repair membrane of layer.Glycerol is the moisturizer of traditional classical, there is good water imbibition, can keep skin wet, softness,
One layer of moisturizing film can be formed on skin, isolation foreign substance invades skin, prevents moisture from evaporating.Patient can be effectively improved
Dry skin, furfur phenomenon promote inflammatory resolution.Damaged skin barrier is repaired, damage of the environmental stimuli to skin is reduced, rises
To anti-inflammatory, anti-allergic effects, while pigmented generation can be reduced.
Although the embodiments of the present invention have been disclosed as above, but its institute not only in the description and the implementation
Column use.It can be applied to various suitable the field of the invention completely.For those skilled in the art, can hold
It changes places and realizes other modification.Therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously
It is not limited to specific details.
Claims (7)
1. a kind of using OPC as the tridecanoic peptide antibacterial repair latex of carrier, which is characterized in that weight percentage, including with
Lower component：Carbomer 0.2%, procyanidine 0.4%-0.8%, polymer hyaluronic acid 0.1%, low molecular weight hyaluronic acid
0.1%, glycerol 3%, tridecanoic peptide 12%-24%, mineral oil 10%, self-emulsifying glycerin monostearate 2%, triethanolamine
0.2%, surplus is purified water；The tridecanoic peptide molecular formula is：
Cys-Phe-Arg-Val-Cys-Tyr-Arg-Gly-Ile-Cys-Tyr-Arg-Cys。
2. according to claim 1 a kind of using OPC as the tridecanoic peptide antibacterial repair latex of carrier, which is characterized in that by weight
Measure percentage composition meter, including following components：Carbomer 0.2%, procyanidine 0.5%, polymer hyaluronic acid 0.1%, low point
Sub- hyaluronic acid 0.1%, glycerol 3%, tridecanoic peptide 15%, mineral oil 10%, self-emulsifying glycerin monostearate 2%, three ethyl alcohol
Amine 0.2%, surplus are purified water；The tridecanoic peptide molecular formula is：
Cys-Phe-Arg-Val-Cys-Tyr-Arg-Gly-Ile-Cys-Tyr-Arg-Cys。
3. according to claim 1 a kind of using OPC as the tridecanoic peptide antibacterial repair latex of carrier, which is characterized in that described
Procyanidine is leaf of Fructus vaccini vitis-idaeae extract (OPC).
4. according to claim 1 a kind of using OPC as the tridecanoic peptide antibacterial repair latex of carrier, which is characterized in that described
Procyanidin content is greater than 95%.
5. it is according to claim 1-4 using OPC as the tridecanoic peptide antibacterial repair latex preparation method of carrier, it is special
Sign is, includes the following steps：
Step 1：Each component content is calculated, the purified water of corresponding amount is added in water phase container；
Step 2：Carbomer, polymer hyaluronic acid are sequentially added into water phase container, low molecular weight hyaluronic acid stirs evenly
Homogeneous is carried out using homogenizer afterwards；
Step 3：OPC is added into water phase container, stirs, adds glycerol, stirs, is then added
Tridecanoic peptide stirs, and the liquid in water phase container is then heated to 70 DEG C, heat preservation；
Step 4：Mineral oil is added into oily phase container, self-emulsifying glycerin monostearate is then added, it will be in oily phase container
Liquid is heated to 75 DEG C, melts self-emulsifying glycerin monostearate, then stirs evenly, and keeps the temperature；
Step 5：Liquid in oily phase container is poured into water phase container, stirs while adding, stirs, is formed
Uniform lotion；
Step 6：The triethanolamine being slowly added into above-mentioned emulsion, stirs while adding, and forms the uniform cream of viscosity qualification
Then liquid cools to room temperature；
Step 7：Sterilizing, it is filling after quality inspection is qualified.
6. a kind of using OPC as the tridecanoic peptide antibacterial repair latex preparation method of carrier according to claim 5, feature exists
In being emulsified in the step 5 using emulsifier.
7. according to claim 1-4 using OPC as the tridecanoic peptide antibacterial repair latex of carrier, preparation dermatitis,
After the sensitivity skin such as eczema and cosmetology, after solarization and physics, chemistry etc. stimulate caused by apply in damaged skin dressing.
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| CN110358801A (en) * | 2019-05-23 | 2019-10-22 | 吉林省瑞金浩业生物科技有限公司 | A kind of fermented by yeast obtains the method and its application of tridecanoic peptide |
| CN115252515A (en) * | 2022-04-02 | 2022-11-01 | 洞玛生物技术(深圳)有限公司 | Skin antibacterial repair functional liquid and preparation method and application thereof |
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