CN105154374A - Heavy metal passivation microbial inoculum and preparation method thereof - Google Patents
Heavy metal passivation microbial inoculum and preparation method thereof Download PDFInfo
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Abstract
The invention provides a heavy metal passivation microbial inoculum. The heavy metal passivation microbial inoculum comprises the following raw materials in parts by weight: 50-60 parts of active components and 40-50 parts of auxiliary materials, wherein the active components comprise pseudomonas putida, yeast and bacillus subtilis; the weight ratio of the pseudomonas putida to the yeast to the bacillus subtilis is 1: (0.8-1.2): (2.5-3.5); the auxiliary materials comprise bran, brown sugar, monopotassium phosphate, calcium carbonate powder and chaff powder; the weight ratio of the bran to the brown sugar to monopotassium phosphate to the calcium carbonate powder to the chaff powder is (35-45): (8-10): 1: (55-65): (55-65). The invention also provides a preparation method of the heavy metal passivation microbial inoculum. The heavy metal passivation microbial inoculum has a relatively good effect on passivating a plurality of heavy metals in soil, and the heavy metals in soil can be transformed into a low-toxicity or nontoxic state.
Description
Technical field
The present invention relates to a kind of can the microbial inoculum and preparation method thereof of heavy metal in passivation soil.
Background technology
Heavy metal in soil such as cadmium, arsenic cannot natural decomposition, and these heavy metals can affect the seed output and quality of farm crop, cause the havoc of the ecosystem, especially can healthy by food chain harm humans.Utilize microbiobacterial agent to be proved to be a kind of effective method to improve Soil structure, but this microbial inoculum generally can not heavy metal in passivation soil.Therefore, a heavy metal species passivation microbial inoculum can be designed, the heavy metal in passivation soil, reduce farm crop to these Metal uptake rates, the final content reducing heavy metal in farm crop.
Summary of the invention
The object of this invention is to provide a heavy metal species passivation microbial inoculum, it has passivation effect preferably to the various heavy in soil, heavy metal in soil can be changed into low toxicity or nontoxic form.
A further object of the invention is the preparation method by a heavy metal species passivation microbial inoculum, and it can provide passivation ability and the passivation efficiency of heavy metal passivation microbial inoculum, reduces the consumption of passivation microbial inoculum.
In order to realize, according to these objects of the present invention and other advantage, providing a heavy metal species passivation microbial inoculum, comprise the raw material of following parts by weight:
Active ingredient 50-60 part and auxiliary material 40-50 part;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:0.8-1.2:2.5-3.5;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 35-45:8-10:1:55-65:55-65.
Preferably, described heavy metal passivation microbial inoculum, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:1:3, and the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 40:9:1:60:60.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
Preferably, the preparation method of described heavy metal passivation microbial inoculum, after interpolation pseudomonas putida, yeast and subtilis, adds the additive of 25-30 part;
Described additive comprises magnet powder end, energy stone powder and Powdered Activated Carbon, and wherein, the weight ratio of described magnetite powder, described energy stone powder and described Powdered Activated Carbon is 1:1:1.
Preferably, the preparation method of described heavy metal passivation microbial inoculum, also comprises:
Mixture equivalent after pulverizing is divided into three parts, then with special device process 30-40 minute;
Described special device is the hollow columnar structures vertically arranged, and closed at both ends, described special device side wall upper part is provided with three opening for feeds, these three opening for feeds are positioned at same level, each opening for feed is all communicated with a feed-pipe, each feed-pipe is all that 45° angle is arranged with vertical direction, and these three feed-pipes are respectively used to the mixture after adding first part of pulverizing, second part of mixture pulverized after rear mixture and the 3rd part of pulverizing; Be provided with a blast inlet in the middle part of described special device sidewall, described blast inlet is communicated with a blast pipe, and described blast pipe is arranged along the tangent to periphery direction of described special device, and described blast pipe is also communicated with a gas blower, for air blast in described special device; Described special device sidewall center outer surface is provided with a ultrasonic generator, for the ultrasonic wave to special device internal irradiation 20MHz; Described special device outside surface bottom is also provided with a discharge port.
Preferably, the preparation method of described heavy metal passivation microbial inoculum, the internal diameter of described special device is 0.8 meter.
The present invention at least comprises following beneficial effect:
(1) composite fungus agent of the present invention has better effects to various heavy in passivation soil, heavy metal in soil can be changed into low toxicity or nontoxic form.Wherein the effect of composite fungus agent passivation cadmium is especially remarkable, and the clearance of heavy metal cadmium reaches 87.63%.Wherein, pseudomonas putida is passivation cadmium element mainly, yeast oxidized portion heavy metal element, and subtilis can absorbed portion heavy metal element.
(2) the present invention is added with composition is magnetite powder, the additive of energy stone powder and Powdered Activated Carbon, first magnetite powder and energy stone powder all have certain magnetic, be sprayed onto after in soil at heavy metal passivation microbial inoculum of the present invention, due to attracting each other between magnetite powder and energy stone powder, active ingredient and auxiliary material long period can be enable to be attached on soil particle to the abundant passivation of the heavy metal in soil simultaneously, which improves passivation ability of the present invention and passivation effect, secondly, Powdered Activated Carbon has stronger adsorptive power, can a certain amount of active ingredient and auxiliary material be adsorbed on around it, more auxiliary material is had around active ingredient, this just makes to there is more nutritive substance around active ingredient, thus there is very high survival rate, thus the usage quantity of fermenting agent can be reduced, cost-saving.In the present invention, the consumption of additive and the ratio of active ingredient are preferably 1:2, and when ratio is less than 1:2, passivation ability of the present invention and passivation effect obviously reduce.
(3) special device of the present invention is for strengthening the degree of mixing of each component in raw material, additive is uniformly distributed in passivation microbial inoculum, at utmost plays the effect of additive.First mixture equivalent is divided into three parts, add in special device from three feed-pipes respectively, mixture runs into the spiralling air-flow blasted in blast pipe in the process fallen, follow flow spiral motion, and under hyperacoustic effect, accelerated motion, each component in such raw material can fully mix.Under the process of special device, additive can be evenly distributed in passivation microbial inoculum, and then plays a great role in heavy metal-passivated process, greatly improves passivation ability and the passivation effect of passivation microbial inoculum.Utilize the preferred time of special device process to be 30-40 minute, more than 40 minutes, ultrasonic wave can destroy active ingredient.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Accompanying drawing explanation
Fig. 1 is the structural representation of special device of the present invention.
Embodiment
Below in conjunction with example, the present invention is described in further detail, can implement according to this with reference to specification sheets word to make those skilled in the art.
Should be appreciated that used hereinly such as " to have ", other element one or more do not allotted in " comprising " and " comprising " term or the existence of its combination or interpolation.
< example 1>
One heavy metal species passivation microbial inoculum, comprises the raw material of following parts by weight:
Active ingredient 55 parts and auxiliary material 45 parts;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:1:3;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 40:9:1:60:60.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
< example 2>
One heavy metal species passivation microbial inoculum, comprises the raw material of following parts by weight:
Active ingredient 50 parts and auxiliary material 50 parts;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:0.8:2.5;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 35:8:1:55:55.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
< example 3>
One heavy metal species passivation microbial inoculum, comprises the raw material of following parts by weight:
Active ingredient 60 parts and auxiliary material 40 parts;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:1.2:3.5;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 45:10:1:65:65.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
< example 4>
One heavy metal species passivation microbial inoculum, comprises the raw material of following parts by weight:
Active ingredient 55 parts and auxiliary material 45 parts;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:1:3;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 40:9:1:60:60.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
After interpolation pseudomonas putida, yeast and subtilis, add the additive of 27.5 parts;
Described additive comprises magnet powder end, energy stone powder and Powdered Activated Carbon, and wherein, the weight ratio of described magnetite powder, described energy stone powder and described Powdered Activated Carbon is 1:1:1.
The preparation method of described heavy metal passivation microbial inoculum, also comprises:
Mixture equivalent after pulverizing is divided into three parts, then with special device process 35 minutes;
As shown in Figure 1, described special device is the hollow columnar structures vertically arranged, and closed at both ends, described special device side wall upper part is provided with three opening for feeds, these three opening for feeds are positioned at same level, each opening for feed is all communicated with a feed-pipe 1, and each feed-pipe 1 is all that 45° angle is arranged with vertical direction, and these three feed-pipes 1 are respectively used to the mixture after adding first part of pulverizing, second part of mixture pulverized after rear mixture and the 3rd part of pulverizing; Be provided with a blast inlet in the middle part of described special device sidewall, described blast inlet is communicated with a blast pipe 2, and described blast pipe 2 is arranged along the tangent to periphery direction of described special device, and described blast pipe 2 is also communicated with a gas blower, for air blast in described special device; Described special device sidewall center outer surface is provided with a ultrasonic generator 3, for the ultrasonic wave to special device internal irradiation 20MHz; Described special device outside surface bottom is also provided with a discharge port.
The internal diameter of described special device is 0.8 meter.
< example 5>
One heavy metal species passivation microbial inoculum, comprises the raw material of following parts by weight:
Active ingredient 50 parts and auxiliary material 50 parts;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:0.8:2.5;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 35:8:1:55:55.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
After interpolation pseudomonas putida, yeast and subtilis, add the additive of 25 parts;
Described additive comprises magnet powder end, energy stone powder and Powdered Activated Carbon, and wherein, the weight ratio of described magnetite powder, described energy stone powder and described Powdered Activated Carbon is 1:1:1.
The preparation method of described heavy metal passivation microbial inoculum, also comprises:
Mixture equivalent after pulverizing is divided into three parts, then with special device process 30 minutes;
Described special device is the hollow columnar structures vertically arranged, and closed at both ends, described special device side wall upper part is provided with three opening for feeds, these three opening for feeds are positioned at same level, each opening for feed is all communicated with a feed-pipe, each feed-pipe is all that 45° angle is arranged with vertical direction, and these three feed-pipes are respectively used to the mixture after adding first part of pulverizing, second part of mixture pulverized after rear mixture and the 3rd part of pulverizing; Be provided with a blast inlet in the middle part of described special device sidewall, described blast inlet is communicated with a blast pipe, and described blast pipe is arranged along the tangent to periphery direction of described special device, and described blast pipe is also communicated with a gas blower, for air blast in described special device; Described special device sidewall center outer surface is provided with a ultrasonic generator, for the ultrasonic wave to special device internal irradiation 20MHz; Described special device outside surface bottom is also provided with a discharge port.
The internal diameter of described special device is 0.8 meter.
< example 6>
One heavy metal species passivation microbial inoculum, comprises the raw material of following parts by weight:
Active ingredient 60 parts and auxiliary material 40 parts;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:1.2:3.5;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 45:10:1:65:65.
The preparation method of one heavy metal species passivation microbial inoculum, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
After interpolation pseudomonas putida, yeast and subtilis, add the additive of 30 parts;
Described additive comprises magnet powder end, energy stone powder and Powdered Activated Carbon, and wherein, the weight ratio of described magnetite powder, described energy stone powder and described Powdered Activated Carbon is 1:1:1.
The preparation method of described heavy metal passivation microbial inoculum, also comprises:
Mixture equivalent after pulverizing is divided into three parts, then with special device process 40 minutes;
Described special device is the hollow columnar structures vertically arranged, and closed at both ends, described special device side wall upper part is provided with three opening for feeds, these three opening for feeds are positioned at same level, each opening for feed is all communicated with a feed-pipe, each feed-pipe is all that 45° angle is arranged with vertical direction, and these three feed-pipes are respectively used to the mixture after adding first part of pulverizing, second part of mixture pulverized after rear mixture and the 3rd part of pulverizing; Be provided with a blast inlet in the middle part of described special device sidewall, described blast inlet is communicated with a blast pipe, and described blast pipe is arranged along the tangent to periphery direction of described special device, and described blast pipe is also communicated with a gas blower, for air blast in described special device; Described special device sidewall center outer surface is provided with a ultrasonic generator, for the ultrasonic wave to special device internal irradiation 20MHz; Described special device outside surface bottom is also provided with a discharge port.
The internal diameter of described special device is 0.8 meter.
In order to effect of the present invention is described, contriver provides comparative experiments as follows:
< comparative example 1>
In the process preparing heavy metal passivation microbial inoculum, do not add additive, identical with example 4 of all the other parameters, technological process is also identical.
< comparative example 2>
In the process preparing heavy metal passivation microbial inoculum, not by the mixture special device process after pulverizing, identical with example 4 of all the other parameters, technological process is also identical.
Choose the arable land of one piece of heavy metal contamination, be divided into 9 pieces of pilot regions, the heavy metal passivation microbial inoculum obtained for arbitrary example and comparative example is all applied to one piece of pilot region and tests, the amount of the passivation microbial inoculum that every block pilot region applies is equal, and last block pilot region only uses the auxiliary material of equivalent as blank group.In arable soil, the content of the exchangeable species of cadmium, lead and copper is respectively 1.80 μ g/g, 40 μ g/g and 15 μ g/g, after four months, chooses soil sample from each pilot region, measures the exchangeable species heavy metal content in soil sample, obtains result as shown in table 1.
[the heavy metal passivation microbial inoculum that each example of table 1 and comparative example obtain is to the disposition of each pilot region soil]
| Cadmium (μ g/g) | Plumbous (μ g/g) | Copper (μ g/g) | |
| Example 1 | 1.61 | 38.5 | 9.50 |
| Example 2 | 1.60 | 38.2 | 9.57 |
| Example 3 | 1.63 | 38.1 | 9.59 |
| Example 4 | 1.42 | 36.5 | 9.32 |
| Example 5 | 1.45 | 36.9 | 9.37 |
| Example 6 | 1.45 | 37.0 | 9.33 |
| Comparative example 1 | 1.59 | 37.7 | 9.48 |
| Comparative example 2 | 1.60 | 37.9 | 9.48 |
| Blank group | 1.80 | 40.0 | 14.90 |
Can find out from upper table 1, the heavy metal in soil is difficult to natural decomposition, must use metal passivator.And due in the process preparing heavy metal passivation microbial inoculum in example 4, with the addition of additive, the clearance of heavy metal passivation microbial inoculum to cadmium, lead and copper that it obtains is significantly higher than the situation of comparative example 1, shows that the passivation ability of heavy metal passivation microbial inoculum is significantly higher than comparative example 1.Due in the process preparing heavy metal passivation microbial inoculum in example 4, due to by pulverize after mixture special device process, it obtains the situation of a little higher than comparative example 2 of the clearance of heavy metal passivation microbial inoculum to cadmium, lead and copper, shows that the passivation ability of heavy metal passivation microbial inoculum is higher than comparative example 2.Visible, the present invention is in the process preparing heavy metal passivation microbial inoculum, and interpolation additive and the mixture after pulverizing with special device process all can significantly improve the passivation ability of heavy metal passivation microbial inoculum.
Although embodiment of the present invention are open as above, it is not restricted to listed in specification sheets and embodiment utilization.It can be applied to various applicable the field of the invention completely.For those skilled in the art, can easily realize other amendment.Therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the example described.
Claims (6)
1. a heavy metal species passivation microbial inoculum, is characterized in that, comprise the raw material of following parts by weight:
Active ingredient 50-60 part and auxiliary material 40-50 part;
Described active ingredient comprises pseudomonas putida, yeast, subtilis, and wherein, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:0.8-1.2:2.5-3.5;
Described auxiliary material comprises wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder, and wherein, the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 35-45:8-10:1:55-65:55-65.
2. heavy metal passivation microbial inoculum as claimed in claim 1, it is characterized in that, the weight ratio of described pseudomonas putida, described yeast and described subtilis is 1:1:3, and the weight ratio of described wheat bran, described brown sugar, described potassium primary phosphate, described Paris white and described chaff powder is 40:9:1:60:60.
3. a preparation method for heavy metal passivation microbial inoculum as claimed in claim 1 or 2, is characterized in that, comprising:
Get wheat bran, brown sugar, potassium primary phosphate, Paris white and chaff powder according to above-mentioned weight ratio, then add pseudomonas putida, yeast, subtilis according to above-mentioned weight ratio, mix, obtain mixture, pulverize, obtain heavy metal passivation microbial inoculum.
4. the preparation method of heavy metal passivation microbial inoculum as claimed in claim 3, is characterized in that, after interpolation pseudomonas putida, yeast and subtilis, adds the additive of 25-30 part;
Described additive comprises magnet powder end, energy stone powder and Powdered Activated Carbon, and wherein, the weight ratio of described magnetite powder, described energy stone powder and described Powdered Activated Carbon is 1:1:1.
5. the preparation method of heavy metal passivation microbial inoculum as claimed in claim 4, is characterized in that, also comprise:
Mixture equivalent after pulverizing is divided into three parts, then with special device process 30-40 minute;
Described special device is the hollow columnar structures vertically arranged, and closed at both ends, described special device side wall upper part is provided with three opening for feeds, these three opening for feeds are positioned at same level, each opening for feed is all communicated with a feed-pipe, each feed-pipe is all that 45° angle is arranged with vertical direction, and these three feed-pipes are respectively used to the mixture after adding first part of pulverizing, second part of mixture pulverized after rear mixture and the 3rd part of pulverizing; Be provided with a blast inlet in the middle part of described special device sidewall, described blast inlet is communicated with a blast pipe, and described blast pipe is arranged along the tangent to periphery direction of described special device, and described blast pipe is also communicated with a gas blower, for air blast in described special device; Described special device sidewall center outer surface is provided with a ultrasonic generator, for the ultrasonic wave to special device internal irradiation 20MHz; Described special device outside surface bottom is also provided with a discharge port.
6. the preparation method of heavy metal passivation microbial inoculum as claimed in claim 5, it is characterized in that, the internal diameter of described special device is 0.8 meter.
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| CN110468080B (en) * | 2019-07-19 | 2024-01-19 | 湖南农业大学 | Microbial agent for promoting rice growth and reducing cadmium as well as preparation method and application method thereof |
| CN110698269A (en) * | 2019-11-05 | 2020-01-17 | 浙江科技学院 | Slow-release type farmland soil heavy metal passivation biochar and preparation method thereof |
| CN110885176A (en) * | 2019-12-11 | 2020-03-17 | 河南莫大生物科技有限公司 | Method for treating and disposing sludge by utilizing microbial flocculant and microbial wall breaking |
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