CN102696923B - Premix compound for aquatic products, application thereof in feed additive, and batch thereof - Google Patents
Premix compound for aquatic products, application thereof in feed additive, and batch thereof Download PDFInfo
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Abstract
The invention discloses premix compound for aquatic products, the application thereof in feed additive and the batch thereof. In the premix compound per kilogram, vitamin B complex accounts for 80 to 140g, composite trace element accounts for 110 to 200g, taurine accounts for 100 to 300g, phytase accounts for 10 to 30g, glutamine accounts for 100 to 300g, DMPT accounts for 5 TO 20 g, composite microbial ecological agent accounts for 1 to 10g, composite traditional Chinese medicine composition accounts for 1 to 10g, and the margin is a carrier. The ingredients of the composite microbial ecological agent include fermented powder of bacillus subtilis CGMCC, No. 4628, fermented powder of bacillus licheniformis CGMCC, No. 5094 and fermented powder of enterococcus faecalis CGMCC, No. 5092. The main ingredients of the composite microbial ecological agent include three probiotics. Not only the probiotic function is achieved, but also the premix compound can be used for purification and conditioning to aquaculture water body, the environmental suitability is strong, the function is stable, and pollutants such as ammonia nitrogen, tric nitrogen, trite nitrogen, COD and the like in the water body are effectively degraded.
Description
Technical field
The present invention relates to feeding micro-ecological technical field, especially a kind of aquatic products is mated material with premix and the application in preparing feeding additive aquatic animal thereof.
Background technology
Along with aquaculture economized degree improves constantly, physicochemical environment and the ecological environment of breeding water body worsen increasingly; Meanwhile, the excess discharges such as human lives's sewage, various industrial wastewater, agriculture water-break, have all aggravated the decline of breeding water body quality; These problems cause aquiculture disease to be on the rise.As: liver and gall syndrome of fish is one of fish disease of frequent generation in recent years, the multiple cultured fishes such as harm carp, crucian carp, grass carp, megalobrama amblycephala, black carp, Tilapia mossambica, as treatment not in time, the death rate can reach 60%-90%, to raiser, has caused serious economic loss.
At present, in order to prevent the generation of fish disease, mainly adopt Chinese herbal medicine, chemical drug prevent and treat or fish material in add probio, its advantage of herbal control is to use safe, pollution-free, noresidue, is difficult for producing drug resistance, shortcoming be take effect slow; The advantage of chemical drug control is instant effect, but large, the residual high fish medicine of side effect does not meet the requirement of society environmental protection yet; Compound micro-ecological preparation is owing to having the intestinal environment of improvement, increase feed, suppress pernicious bacteria, improving many-sided effects such as water quality simultaneously, more and more by people, be applied in culture fishery, but still there is the problems such as bacterial classification unstable properties, function singleness and environmental adaptability be poor in a lot of preparations, make it can not adapt to the environment that water body is complicated and changeable, after imposing on water body, effect is unstable, thereby has limited probiotics applying in aquaculture.
Summary of the invention
Technical problem to be solved by this invention is, a kind of aquatic products premix is provided, in premix, comprise the compound Chinese herb that can prevent that fish disease from occurring, especially prevent the generation of liver and gall syndrome of fish, comprise compound micro-ecological preparation, compound micro-ecological preparation main component is three kinds of probios simultaneously.Not only the prebiotic function of tool, also can be used for water body purification of aquaculture and conditioning, and environmental suitability is strong, function-stable and the pollutants such as ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and COD that can effectively degrade in water body.
In order to solve the problems of the technologies described above; the technical solution used in the present invention is: a kind of aquatic products premix; in every kilogram of premix: B B-complex 80-140g, composite trace element 110-200g, taurine 100-300g, phytase 10-30g, glutamine 100-300g, DMPT 5-20g, compound micro-ecological preparation 1-10g, compound Chinese medicine composition 1-10g, surplus is carrier.
Described B B-complex is prepared from by the composition of following weight proportioning: retinol1 .3%, neo dohyfral D3 0.4%, vitamin e1 8.5%, prokeyvit 2.2%, VB11 %, vitamin B2 1.4%, pyridoxamine 1.1%, cobalamin 0.3%, nicotinic acid 3.2%, calcium pantothenate 2.4%, folic acid 0.2%, biotin 1%, vitamin C 53%, inositol 14%.
The composition of described compound micro-ecological preparation is that the bacterium powder of bacillus subtilis CGMCC No.4628 is, the bacterium powder of the bacterium powder of bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092.
The medicine material of described compound Chinese medicine composition is proportioning by weight: rheum officinale 30-60 part, Herba Andrographitis 30-60 part, cape jasmine 30-70 part, Artemisia capillaris 50-80 part, Radix Glycyrrhizae 10-30 part.
Described composite trace element is prepared from by the composition of following weight proportioning: copper methionine 0.75%, methionine iron 24.4%, zinc methionine 5%, manganese methionine 2.7%, magnesium sulfate monohydrate 67%, KI 0.02%, yeast selenium 0.03%, waterless cobaltous chloride 0.1%.
Copper content >=10% in described copper methionine; Iron content >=10% in described methionine iron; Zinc content >=10% in described zinc methionine; Manganese content >=10% in described manganese methionine; Se content >=1000mg/kg in described yeast selenium, described percentage is mass percent.
In described compound micro-ecological preparation, the bacterium opaque amount proportioning of the bacterium powder of the bacterium powder of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092 is 1-5:1-5:2-7.
Described phytase is high temperature resistant neutral phytase, and activity is 5000U/g; Active constituent content >=99% in described taurine; Described glutamine purity >=99%; Described DMPT purity >=99%, described percentage is mass percent; Described carrier is rice chaff or zeolite powder.
Above-mentioned aquatic products is the application in preparing feeding additive aquatic animal with premix.
Comprise the batch of premix for above-mentioned aquatic products, the interpolation percentage by weight of premix in batch is 1-10 ‰.
The invention has the beneficial effects as follows:
1, the present invention is combined compound micro-ecological preparation with multiple medicine pharmaceutical composition, preparation is containing three kinds of high-quality probios, its bacterial classification chamber in strict accordance with microecology rule choose seeds, seed selection, guarantee the good of bacterial classification, viable bacteria content is high, has the growth of good micro-Ecological regulation, optimizing water quality, promotion aquatic livestock, the effect of enhancing immunity, goes back the liver and gall cell of repairing damaged in the time of control fish disease, good effect, instant effect; Use safely, have no side effect, can not produce harm humans health medicament residue, be difficult for producing drug resistance, cause drug induced disease; And steady quality, safe and reliable, environmental protection, pollution-free.
2, preparation of the present invention can significantly promote the individual weight gain of aquatic livestock, increase the yield per unit area and total amount, thereby the income of increase aquaculture is increased the benefit.
3, high, the long shelf-life of viable bacteria content of the present invention, sphere of action is large, production cost is low, be to improve specially water quality, increase the desirable feed that aquatic livestock immunity and control aquatic product disease occur.
4, premix provided by the invention can make the consumption of fish meal in feed be reduced to 2% from 6%, reduced by approximately 67% fish meal consumption, reduced the dependence of fish feed to fish meal, reduce costs simultaneously, aminoacid chelating microelement and phytase have also been used, can effectively improve the digestive utilization ratio of trace element and phytate phosphorus, carp is respond well after using; , reduced the discharge of water environment and pollution meanwhile, met the requirement of current green cultivation.
The specific embodiment
Below in conjunction with the specific embodiment, the present invention is described in further detail:
Aquatic products premix of the present invention; in every kilogram of premix: B B-complex 80-140g, composite trace element 110-200g, taurine 100-300g, phytase 10-30g, glutamine 100-300g, DMPT5-20g, percentage by weight are that 1-10 ‰ compound micro-ecological preparation, percentage by weight are the compound Chinese medicine component thing of 1-10 ‰, and surplus is carrier.
Further; in every kilogram of premix: B B-complex 120g, composite trace element 150g, taurine 200g, phytase 20g, glutamine 200g, DMPT 10g, percentage by weight are that 1-5 ‰ compound micro-ecological preparation, percentage by weight are the compound Chinese medicine composition of 1-5 ‰, and surplus is carrier.
Wherein, described B B-complex is prepared from by the composition of following weight proportioning: retinol1 .3%, neo dohyfral D3 0.4%, vitamin e1 8.5%, prokeyvit 2.2%, VB11 %, vitamin B2 1.4%, pyridoxamine 1.1%, cobalamin 0.3%, nicotinic acid 3.2%, calcium pantothenate 2.4%, folic acid 0.2%, biotin 1%, vitamin C 53%, inositol 14%.
The main component of compound micro-ecological preparation is bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092.
Compound Chinese medicine composition mainly by: rheum officinale 30-60 part, Herba Andrographitis 30-60 part, cape jasmine 30-70 part, Artemisia capillaris 50-80 part, Radix Glycyrrhizae 10-30 part form.
Further, 50 parts of rheum officinales, 50 parts of Herba Andrographitis, 50 parts of cape jasmines, 70 parts of Artemisia capillaris, 20 parts, Radix Glycyrrhizae.
Wherein, in preparation of the present invention, the bacterium opaque amount proportioning of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCCNo.5094 and enterococcus faecalis CGMCC No.5092 is 1-5:1-5:2-7.
Further, in preparation of the present invention, the bacterium opaque amount proportioning of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092 is 1:1:2.
Wherein, described composite trace element is prepared from by the composition of following weight proportioning: copper methionine 0.75%, methionine iron 24.4%, zinc methionine 5%, manganese methionine 2.7%, magnesium sulfate monohydrate 67%, KI 0.02%, yeast selenium 0.03%, waterless cobaltous chloride 0.1%.
Wherein, copper content >=10% in described copper methionine; Iron content >=10% in described methionine iron; Zinc content >=10% in described zinc methionine; Manganese content >=10% in described manganese methionine; Se content >=1000mg/kg in described yeast selenium.
Wherein, in order to guarantee the result of use of phytase in the present invention, described phytase is high temperature resistant neutral phytase, and activity is 5000U/g.In order to guarantee attractant and the property balanced in nutrition of this product, active constituent content >=99% in above-mentioned taurine; Above-mentioned glutamine purity >=99%; Above-mentioned DMPT purity >=99%.
Wherein, described carrier is rice chaff or zeolite powder.
The interpolation percentage by weight of premix in batch is 1-10 ‰.
Bacillus licheniformis of the present invention (Bacillus licheniformis) is applicant's separation from Roll road, through directed primary dcreening operation and multiple sieve, obtains.Its vegetative cell is shaft-like, and its gemma is oval or long tubular, is middle life or the life of inferior end, and sporangiocyst slightly expands.Isolate or be short chain, rod end is semicircle.In 12～16h, can form bacterium colony, bacterium colony is circular, or irregular, and edge is hair-like, and bacteria colony white is opaque, corrugationless.Gram-positive.Applicant has carried out the experiments such as acid resistance, bile tolerance, heat-resisting quantity, fermentation character and bacteriostatic activity to this bacillus licheniformis, this bacillus licheniformis has the features such as strong to contrary environmental resistance, adhesiveness strong, growth fast, biomass is large, bacteriostatic activity is good.Bacillus licheniformis of the present invention (Bacillus licheniformis) applicant has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 26th, 2011, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, deposit number is CGMCCNo.5094.Enterococcus faecalis of the present invention (Enterococcus faecalis) obtains for applicant's separation from Radix Polygalae Crotalarioidis, and its biological property is as follows: bacterium colony rounding, and smooth surface, opaque, milky, the smooth of the edge, gram-positive bacteria; Single thalline is spherical or ellipticity.Enterococcus faecalis of the present invention is through simulated gastric fluid, bile fluid and stable on heating screening; Again through enzymatic productivity screening and bacteriostasis screening, can tolerate pH2.0,1% pepsic simulated gastric fluid, can tolerate 0.3% artificial bile fluid, can tolerate the pelleting temperature of 85 ℃, also can suppress enteropathogenic E．Coli K88, K99 and staphylococcus aureus, have stronger product acid and the ability that suppresses mould in dregs of beans, cotton dregs, maize straw.Enterococcus faecalis of the present invention (Enterococcus faecalis) applicant has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 26th, 2011, be called for short CGMCC, and address is the same, and deposit number is CGMCC No.5092.The present invention bacillus subtilis (Bacillussubtilis) used obtains for applicant's separation from traditional zymotic fermented soya bean, and its biological property is as follows: bacterium colony rough surface, and opaque, dirty white, bacterium colony is circular, edge indentation, gram-positive bacteria; Gemma form is oval to column, is positioned at thalline central authorities or slightly inclined to one side, and after sporulation, thalline does not expand.Bacillus subtilis of the present invention is significantly different from existing bacillus subtilis, can tolerate pH2.0,1% pepsic simulated gastric fluid, can tolerate 0.3% artificial cholate, can tolerate the pelleting temperature of 80 ℃, can suppress enteropathogenic E．Coli K88, K99 and staphylococcus aureus, the ability with stronger cellulase-producing, can degraded cellulose.Bacillus subtilis of the present invention (Bacillus subtilis) has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on March 2nd, 2011, be called for short CGMCC, and address is the same, and deposit number is CGMCC No.4628.In the case that above-mentioned bacterial classification was applied for before the applicant, will disclose.
Below in conjunction with specific embodiment, be elaborated:
In following embodiment, B B-complex is prepared from by the composition of following weight proportioning: retinol1 .3%, neo dohyfral D3 0.4%, vitamin e1 8.5%, prokeyvit 2.2%, VB11 %, vitamin B2 1.4%, pyridoxamine 1.1%, cobalamin 0.3%, nicotinic acid 3.2%, calcium pantothenate 2.4%, folic acid 0.2%, biotin 1%, vitamin C 53%, inositol 14%.
The preparation method of compound Chinese medicine composition: take by weight ratio each Chinese medicine material, rheum officinale 30-60 part, Herba Andrographitis 30-60 part, cape jasmine 30-70 part, Artemisia capillaris 50-80 part, Radix Glycyrrhizae 10-30 part, ultramicro grinding, sieve, must be less than 50 microns of powder, evenly mix, obtain.
The preparation method of the bacterium powder of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092 is as follows:
The preparation of enterococcus faecalis CGMCC 5092 bacterium powder:
Seed culture medium is commodity MRS meat soup (lactic acid bacteria special culture media).
Condition of culture: 37 ℃, 100rpm, 12h.
Enterococcus faecalis fermentation culture medium: 3.5% peptone, 6.0% powdered beef, 0.50% dibasic ammonium citrate, 3.5% sucrose, 1.0% yeast soaks powder, 0.4% dipotassium hydrogen phosphate, 0.1% sodium acetate, 0.02% magnesium sulfate, 0.005% manganese sulfate, 0.05% Tween 80.
Condition of culture: 37 ℃, 50rpm, 12h.
Gained zymotic fluid is centrifugal with high speed freezing centrifuge, centrifugal condition: 5000rpm, 4 ℃, 10min.After centrifugal, abandon supernatant, obtain bacterium mud.Use 5.0% sucrose, 1.0% sodium glutamate, 1.0% Vitamin C aqueous acid stirs bacterium mud and mixes, then adds 50% import skimmed milk power.First be placed in-80 ℃ of ultra low temperature freezers freezing, then be placed in the dry 24-30h of vacuum freeze drier, take out and pulverize, cross 60 mesh sieves.
Bacillus licheniformis CGMCC 5094;
Seed culture medium: 0.5% beef extract, 1.0% peptone, 0.5% sodium chloride.
Condition of culture: 37 ℃, 180rpm, 24h.
Bacillus licheniformis culture medium: 3.0% brown sugar, 1.0% corn flour, 3.0% beancake powder, 0.25% urea, 0.15% sodium dihydrogen phosphate.
Condition of culture: 37 ℃, 180rpm, 24h.
Gained zymotic fluid is centrifugal with high speed freezing centrifuge, centrifugal condition: 5000rpm, 4 ℃, 10min.After centrifugal, abandon supernatant, obtain bacterium mud.Use 5.0% sucrose, 1.0% sodium glutamate, 1.0% Vitamin C aqueous acid stirs bacterium mud and mixes, then adds 50% import skimmed milk power.First be placed in-80 ℃ of ultra low temperature freezers freezing, then be placed in the dry 24h of vacuum freeze drier, take out and pulverize, cross 60 mesh sieves.
The preparation of bacillus subtilis CGMCC 4628 bacterium powder.
Seed culture medium: 0.5% beef extract, 1.0% peptone, 0.5% sodium chloride.
Condition of culture: 37 ℃, 180rpm, 24h.
Bacillus subtilis bacterium culture medium: 2.0% glucose, 4.0% soy peptone, 0.16% magnesium sulfate, 0.3% dipotassium hydrogen phosphate, 0.3% potassium dihydrogen phosphate.
Condition of culture: 37 ℃, 180rpm, 24h.
Gained zymotic fluid is centrifugal with high speed freezing centrifuge, centrifugal condition: 5000rpm, 4 ℃, 10min.After centrifugal, abandon supernatant, obtain bacterium mud.Use 5.0% sucrose, 1.0% sodium glutamate, 1.0% Vitamin C aqueous acid stirs bacterium mud and mixes, then adds 50% import skimmed milk power.First be placed in-80 ℃ of ultra low temperature freezers freezing, then be placed in the dry 24h of vacuum freeze drier, take out and pulverize, cross 60 mesh sieves.
Embodiment 1
Formula (1kg content): B B-complex 80g, composite trace element 200g, taurine 300g, phytase 10g, glutamine 100g, DMPT(dimethyl-β-DMPT) 5g, 5g compound Chinese medicine composition (30 parts of rheum officinales, 60 parts of Herba Andrographitis, 70 parts of cape jasmines, 50 parts of Artemisia capillaris, 10 parts, Radix Glycyrrhizae), (the bacterium opaque amount proportioning of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCCNo.5094 and enterococcus faecalis CGMCC No.5092 is 1:1:2 to 5g compound micro-ecological preparation; mass ratio), surplus rice chaff.
First by 180g rice chaff, B B-complex is diluted in advance, join in remaining rice chaff, and then add successively DMPT, phytase, taurine, glutamine, composite trace element, compound Chinese medicine composition, compound micro-ecological preparation; After mixing (coefficient of variation is not more than 5%) is even, obtain fish compound premix of the present invention.
Embodiment 2
Formula (1kg content): B B-complex 110g, composite trace element 170g, taurine 200g, phytase 20g, glutamine 200g, DMPT(dimethyl-β-DMPT) 10g, 1g compound Chinese medicine composition (60 parts of rheum officinales, 30 parts of Herba Andrographitis, 30 parts of cape jasmines, 80 parts of Artemisia capillaris, 130 parts of compositions of Radix Glycyrrhizae), 10g compound micro-ecological preparation (the bacterium opaque amount proportioning of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092 is 5:5:7), surplus rice chaff.
First by 180g rice chaff, B B-complex is diluted in advance, join in remaining rice chaff, and then add successively DMPT, phytase, taurine, glutamine, composite trace element, compound Chinese medicine composition, compound micro-ecological preparation; Mix even after, obtain fish compound premix of the present invention.
Embodiment 3
Formula (1kg content): B B-complex 140g, composite trace element 110g, taurine 100g, phytase 30g, glutamine 300g, DMPT(dimethyl-β-DMPT) 20g, 50 parts of composite Chinese herbal 4g(rheum officinales, 50 parts of Herba Andrographitis, 50 parts of cape jasmines, 70 parts of Artemisia capillaris, 20 parts, Radix Glycyrrhizae), compound micro-ecological preparation 4g (the bacterium opaque amount proportioning of CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092 is 1:1:2), surplus is carrier.
First by 180g rice chaff, B B-complex is diluted in advance, join in remaining rice chaff, and then add successively DMPT, phytase, taurine, glutamine, composite trace element, compound Chinese medicine composition, compound micro-ecological preparation; Mix even after, obtain fish compound premix of the present invention.
Experimental example 1
This test is carried out in great Bei agriculture aquatic products proving ground, Tangshan, totally 4 test group, and 1 control group and 3 embodiment groups, control group is identical with embodiment group basic components, but in feed, does not add compound medicament composition and compound probiotics.The premix of three embodiment group uses is respectively the premix of the embodiment of the present invention 1,2,3 preparations.Each test group is established 4 repetitions, and totally 16 breeding barrels (800L/ bucket), put 30 tail carps (50.13 ± 0.15 grams) for every barrel.To throw the rate of raising during cultivation day be body weight 3%, and respectively throw something and feed once morning and afternoon, within every two weeks, weighs and adjust a feeding volume.Change water 1/3 every afternoon, to guarantee that breeding water body meets the requirement of fishery cultivating water standard, after off-test, get at random the growth indexes such as 3 tail fishes mensuration body length, body weight for every barrel.
The survival rate of respectively organizing carp after off-test is 100%, and its growth data measurement result is in Table 2:
Each test group growth indexes of table 1
| Group | Fish body initial weight (g) | Fish body end heavy (g) | Food ration (g) | Rate of body weight gain % | Feed coefficient | Coefficient of condition % |
| Control group | 50.21±0.12 | 72.21±2.54 | 1710.24±60.34 | 43.82±4.05 a | 2.25±0.14 a | 2.52±0.08 a |
| Embodiment 1 | 50.10±0.25 | 82.78±3.15 | 1663.33±68.22 | 65.19±5.25 c | 2.03±0.21 bc | 2.48±0.11 a |
| Embodiment 2 | 50.08±0.15 | 85.38±3.07 | 1736.88±67.18 | 78.11±5.19 d | 1.78±0.25 c | 2.63±0.13 a |
| Embodiment 3 | 50.11±0.06 | 83.48±2.99 | 1717.98±80.81 | 72.13±4.97 cd | 1.77±0.15 c | 2.61±0.05 a |
Note: the different subscript letter representation significant differences (P<0.05) of tool in same row.
From rate of body weight gain, the rate of body weight gain of 3 embodiment groups of the present invention is all significantly higher than control group; Feed coefficient aspect, 3 embodiment groups are also lower than control group, and embodiment 2 and 3 groups are significantly lower than control group 1 and 2(P<0.05); From coefficient of condition, between each group, there is no notable difference.From above analysis result, use premix of the present invention, can obviously promote the growth of fish, reduce feed coefficient.
The premix of experimental example 2, the embodiment of the present invention 3 preparations is to the result for the treatment of of liver and gall syndrome and the observation to fishpond
Choosing area is 10～25 mu of fishing breeding ponds 70 such as grade, and the depth of water is 1～2 meter; Select the pond that liver and gall syndrome has occurred in various degree to carry out therapeutic test, totally 44 mouthfuls, the feed of the premix of interpolation embodiment 3 preparations of throwing something and feeding respectively, throws something and feeds 7 days continuously, and the method that interval one Zhou Houzai throws something and feeds 7 days is carried out disease treatment.Record cultivation situation and incidence every day.The pond of liver and gall syndrome does not occur as blank, the feed of throwing something and feeding does not comprise compound micro-ecological preparation and combination drug composition.For the pond that liver and gall pathology occurs in various degree, result for the treatment of refers to table 2.
The treatment results of table 2 pair liver and gall syndrome
Result shows, the premix of embodiment 3 preparations has significant therapeutic effect to diseases such as liver and gall syndrome and Secondary cases enteritis thereof, ascites, hemorrhage, ulcer.
The fishpond result of use of table 3 compound premix of the present invention
As seen from the results in Table 3, the nitrogenous source pollutant load in contrast pond presents the trend of rising, and all decrease its imido nitrogen, nitrate, nitrate down ratio of the treatment pond pollutant load in the pond of the premix of embodiment 3 preparation of throwing something and feeding is respectively 42.4%, 74.3% and 4.28%.
In sum, content of the present invention is not limited in the above-described embodiment, and the knowledgeable people in same area can propose easily other embodiment within technological guidance's thought of the present invention, but this embodiment comprises within the scope of the present invention.
Claims (7)
1. an aquatic products premix, it is characterized in that, in every kilogram of premix: B B-complex 80-140g, composite trace element 110-200g, taurine 100-300g, phytase 10-30g, glutamine 100-300g, DMPT5-20g, compound micro-ecological preparation 1-10g, compound Chinese medicine composition 1-10g, surplus is carrier; The composition of described compound micro-ecological preparation is the bacterium powder of bacillus subtilis CGMCC No.4628, the bacterium powder of the bacterium powder of bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092, and in described compound micro-ecological preparation, the bacterium opaque amount proportioning of the bacterium powder of the bacterium powder of bacillus subtilis CGMCC No.4628, bacillus licheniformis CGMCC No.5094 and enterococcus faecalis CGMCC No.5092 is 1-5:1-5:2-7; The medicine material of described compound Chinese medicine composition is proportioning by weight: rheum officinale 30-60 part, Herba Andrographitis 30-60 part, cape jasmine 30-70 part, Artemisia capillaris 50-80 part, Radix Glycyrrhizae 10-30 part.
2. aquatic products premix according to claim 1, is characterized in that, described B B-complex is prepared from by the composition of following weight proportioning: retinol1 .3%, vitamin D
30.4%, vitamin e1 8.5%, vitamin K
32.2%, Cobastab
11%, Cobastab
21.4%, Cobastab
61.1%, Cobastab
120.3%, nicotinic acid 3.2%, calcium pantothenate 2.4%, folic acid 0.2%, biotin 1%, vitamin C 53%, inositol 14%.
3. aquatic products premix according to claim 1, it is characterized in that, described composite trace element is prepared from by the composition of following weight proportioning: copper methionine 0.75%, methionine iron 24.4%, zinc methionine 5%, manganese methionine 2.7%, magnesium sulfate monohydrate 67%, KI 0.02%, yeast selenium 0.03%, waterless cobaltous chloride 0.1%.
4. aquatic products premix according to claim 3, is characterized in that, copper content >=10% in described copper methionine; Iron content >=10% in described methionine iron; Zinc content >=10% in described zinc methionine; Manganese content >=10% in described manganese methionine; Se content >=1000mg/kg in described yeast selenium, described percentage is mass percent.
5. aquatic products premix according to claim 1, is characterized in that, described phytase is high temperature resistant neutral phytase, and activity is 5000U/g; Active constituent content >=99% in described taurine; Described glutamine purity >=99%; Described DMPT purity >=99%, described percentage is mass percent; Described carrier is rice chaff or zeolite powder.
6. the aquatic products of claim 1-5 any one application in preparing feeding additive aquatic animal with premix.
7. comprise a batch for premix for aquatic products described in claim 1-5 any one, it is characterized in that, the interpolation percentage by weight of premix in batch is 1-10 ‰.
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