CN105265762A - Method for producing animal feed based on detoxifying techniques of heat treatment, chemical treatment and microbiological treatment on papayas - Google Patents

Method for producing animal feed based on detoxifying techniques of heat treatment, chemical treatment and microbiological treatment on papayas Download PDF

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Publication number
CN105265762A
CN105265762A CN201410319530.2A CN201410319530A CN105265762A CN 105265762 A CN105265762 A CN 105265762A CN 201410319530 A CN201410319530 A CN 201410319530A CN 105265762 A CN105265762 A CN 105265762A
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China
Prior art keywords
treatment
pawpaw
papayas
animal feed
heat treatment
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CN201410319530.2A
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Chinese (zh)
Inventor
刁丽
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Chengdu Ridong Technology Co Ltd
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Chengdu Ridong Technology Co Ltd
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Priority to CN201410319530.2A priority Critical patent/CN105265762A/en
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Abstract

The invention relates to a processing method of an animal feed, and discloses a method for producing the animal feed based on the detoxifying techniques of heat treatment, chemical treatment and microbiological treatment on papayas. The method comprises the heat treatment, the chemical treatment and microbiological treatment, wherein the heat treatment is fumigation and steaming with high-temperature steam, the chemical treatment is maceration with composite mineral substances, and the microbiological treatment is solid state fermentation with saccharomyces cerevisiae and aspergillus oryzae. Through composite detoxification, the content of free gossypol in the papayas can be greatly reduced and reaches the raising standard; besides, due to the introduction of a microorganism detoxifying method, the heat treatment time can be effectively shortened, the addition of chemical medicines can be effectively reduced, and the nutrient value of the papayas is increased.

Description

Based on the method for pawpaw reason, change, microbial detoxification technology production animal feed
Technical field
The present invention relates to the processing method of animal feed, particularly relate to the method utilizing pawpaw to produce feed.
Background technology
China produces pawpaw more than 1,000 ten thousand tons per year, and pawpaw take pawpaw as raw material, through shelling (part dehulled), removes suede, gets the byproduct after oil with extraction or precompressed lixiviate.Pawpaw is a kind of plant protein fodder resource of great exploitation potential for its, and the Papain matter content being used as feedstuff is at present generally about 40%, and the cotton cypress thick protein that the cotton benevolence shelled completely is made is even up to 50%.From amino acid composition, except methionine is slightly low, all the other essential amino acids all reach the standard that FAO (Food and Agriculture Organization of the United Nation) and the World Health Organization (FA0/WH0) are recommended, and therefore Papain is a kind of important feed protein resource.
Containing gossypol to animal pest in pawpaw, limit it to a great extent and use, be less than 30% of total amount as feed, majority is used as fertilizer for fertilizing the soil, and causes protein resource to waste.Gossypol is a kind of water insoluble and yellowish-brown that is that be dissolved in organic solvent gathers phenol pigment, and accounting for 0.7% 4.8% of pawpaw weight, is the many isocompounds of a kind of polyhydroxy, can be divided into free Mian Chou and in conjunction with Mian Chou by its existence form.Free cotton Chou also claims from by cotton Chou (FreeGossypol, FG), and usually accounting for 0.8% 0.9% of cotton benevolence gross weight, is a kind of multi-element compounds, the toxic effect because having active aldehyde radical and hydroxyl.
In liquefaction process, owing to steaming the heat effects such as stir-fry, squeezing, most of gossypol is combined with amino acid and becomes bound gossoypol, and bound gossoypol is not absorbed by animal in animal alimentary canal, therefore toxicity is little.Another part gossypol is then present in cypress and oil product larger to animal toxicity in a free form, especially nonruminant excess ingestion or picked-up the time longer, can cause growth retardation, reproductive performance and produce and can decline, even cause death, the tolerance of young animal to gossypol is lower.Therefore, when using cotton cypress, be limited the quantity according to the amount of feed object and cotton cypress Free Gossypol or being carried out detoxification treatment.The United Nations's food and food and agricultural organization's regulation, FG<125mg/kg in animal diets, World Health Organization recommended standard regulation FG<400mg/kg.
Summary of the invention
The object of this invention is to provide a kind of pawpaw physics, chemistry and microorganism compound detoxification method, three kinds of poison-removing methods are organically combined, under the prerequisite ensureing higher virus elimination rate, keeps pawpaw nutrient to be worth as far as possible, realize detoxification and guarantee the quality jointly realizing, thus final acquisition high-quality animal feed.
In order to achieve the above object, a kind of method based on pawpaw reason, change, microbial detoxification technology production animal feed of the present invention comprises the steps:
Step1, by pawpaw and: the weight ratio of wheat bran: corn flour=5 7:1 3:1 2 prepares 40 100 object mixed powders, with the conditioning of convenient subsequent material, steam treatment and fermentation process.
Step2, prepare composite mineral matter solution, proportioning is as follows:
NaN0 3 1?5g/L，
012] MgS0 4· 7H 20 0.5?3g/L，
k 2hpo 4 1?5g/L，
NaCl 0.2?1.2g/L，
FeS0 4 0.5?2.5g/L，
MnS0 4 0.1?1g/L，
CuS0 4· 5H 20 0.1?0.8g/L，
Step3, use composite mineral matter solution humidifying mixed powder, control material-water ratio at 1:0.4 1.2 (material-water ratio refers to composite mineral matter liquor capacity and mixed powder mass ratio), obtain mixture.
Step4, at 80 130 DEG C, steam treatment is carried out 10 60 minutes to said mixture, reach the object of first one-step physical detoxification.
After Step5, mixture are cooled to 15 50 DEG C, be 3.0 5.8X10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml (connects bacterium amount by the bacterium amount that the connects access of 2 10% and refers to that saccharomycete nutrient solution volume accounts for mixture quality percentage, both saccharomycete nutrient solution volume: mixed powder quality=0.02 0.1:1), 15 35 DEG C of bottom fermentations 12 48 hours.
Step6, by spore concentration 3.5 6.0X10 8the aspergillus oryzae spore liquid of cfu/ml by 1 7% the bacterium amount that connects access, 15 40 DEG C of bottom fermentations 20 60 hours.
Under preferred embodiment, when the step St print 2 described composite mineral matter solution of preparation, add iron filings, be oxidized to prevent ferrous sulfate.In addition, preferred deionized water preparation composite mineral matter solution.
In order to detect the detoxification efficiency of animal feed product, after above-mentioned St print 6, under 40 80 DEG C of environment, mixture can be dried, to measure the content of free gossypol.
In addition, the present invention is based on the method for pawpaw reason, change, microbial detoxification technology production animal feed, above-mentioned steps is made further improvements, comprises the steps:
Step1, by pawpaw: the weight ratio of wheat bran: corn flour=5.5 6.5:1.5 2.5:1.3 1.7 prepares 60 80 object mixed powders;
Step2, prepare composite mineral matter solution, proportioning is as follows:
NaN0 31.5?3.5g/L,
MgS0 4·7H 200.8?1.8g/L,
K 2HP0 41.5?3g/L，
NaCl0.5?1.lg/L,
FeS0 41.5?2.2g/L,
MnS0 40.5?0.7g/L,
CuS0 4·5H 200.35?0.45g/L；
Step3, control composite mineral matter liquor capacity and mixed powder mass ratio are 1:0.6 1, and damping mixed powder obtains mixture;
Step4, at 100 110 DEG C, the steam treatment step of 30 40 minutes is carried out to mixture;
After Step5, mixture are cooled to 35 45 DEG C, be 3.1 4.0X10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml by 4 8% the bacterium amount that connects access, 20 30 DEG C of bottom fermentations 20 40 hours;
Step6, by spore liquid concentration 3.6 4.5X10 8the aspergillus oryzae spore liquid of cfu/ml by 3 5% the bacterium amount that connects access, 22 36 DEG C of bottom fermentations 22 40 hours.
Under prior art, according to facts have proved, high-temperature steam and composite mineral matter process all can make the free gossypol content in pawpaw reduce, and microbial method utilizes in thalli growth process to reach detoxification efficiency to the Sum decomposition effect that utilizes of free gossypol.The present invention is based on pawpaw reason, change, the feature of methods combining three kinds of methods that microbial detoxification technology produces animal feed, adopt composite detoxicity method.Pawpaw Free Gossypol content can be made greatly to reduce by compound detoxification, reach feeding standard, simultaneously because the introduction of microbiological detoxifying method can the interpolation of effectively shortening heat processing time and minimizing chemicals, improve the nutritive value of pawpaw.Specifically, compared with prior art, tool of the present invention has the following advantages:
1. centered by microbe fermentation method, the advantage of bond logos and chemical method carries out detoxification research to pawpaw, and then shortens the fermentable cycle, shortens heat treatment time in Physical process simultaneously and reduces the addition of chemicals in chemical method.
2. for the bad smell that pawpaw in the past produces after mold fermentation, the present invention adopts mixed fungus fermentation, adds saccharomyces cerevisiae, the alcohol fragrance flavouring utilizing yeast to produce during the fermentation at earlier fermentation, thus reaches the object strengthening palatability.
3. microbial method is comparatively simple for the requirement of equipment, and it is simple that the physics and chemistry method that the present invention simultaneously adopts all has technique, and feature easy and simple to handle, decreases equipment cost, enhances one's market competitiveness.
4. heat treatment, chemicals are added and are produced without the Industrial " three Waste " such as waste water, waste gas in solid ferment process, decrease the expense that process is discharged, and also meet the theory of energy-conserving and environment-protective that country advocates, sustainable development.
Detailed description of the invention
Embodiment 1: by pawpaw: wheat bran: the weight ratio of corn flour=5:3:1 prepares 40 object mixed powders.Composite mineral matter solution ratio is 3g/LNaN0 3, 0.5g/LMgS0 47H 20,4g/LK 2hP0 4, 0.2g/LNaCl, lg/LFeS0 4, 0.4g/LMnS0 4and 0.lg/LCuS0 45H 20, with this composite mineral matter solution humidifying mixed powder, make material-water ratio reach 1:0.4.80 DEG C of steam sterilizations 60 minutes.Be 3.0X10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml is with the mixed powder being cooled to 30 DEG C after the bacterium amount that the connects access steam treatment of 10%, and 15 DEG C ferment 48 hours.Then accessing concentration by 1% is 6.0X10 8the aspergillus oryzae spore liquid of cfu/ml, continues fermentation 60 hours at 15 DEG C.
Embodiment 2: by pawpaw: wheat bran: the weight ratio of corn flour=6:2:2 prepares 40 object mixed powders.Composite mineral matter solution ratio is 4g/LNaN0 3, 2g/LMgS0 47H 20, lg/LK 2hP0 4, 0.8g/LNaCl, 0.5g/LFeS0 4, 0.lg/L111^0 4and 0.5g/LCuS0 45H 20, with this composite mineral matter solution humidifying mixed powder, make material-water ratio reach 1:0.9.120 DEG C of steam sterilizations 20 minutes.Be 4.6X10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml is with the mixed powder being cooled to 50 DEG C after the bacterium amount that the connects access steam treatment of 5%, and 38 DEG C ferment 12 hours.Then accessing concentration by 7% is 3.5X10 8the aspergillus oryzae spore liquid of cfu/ml, continues fermentation 24 hours at 40 DEG C.
Embodiment 3: by pawpaw: wheat bran: the weight ratio of corn flour=5.5:3.6:1.8 prepares 40 object mixed powders.Composite mineral matter solution ratio is 5g/LNaN0 3, 2.5g/LMgS0 4* 7H 20,3g/LK 2hP0 4, 0.5g/LNaCl, 1.5g/LFeS0 4, lg/LK 2hP0 4with 0.6 8/ 1CuS0 4* 5H 20, with this composite mineral matter solution humidifying mixed powder, make material-water ratio reach 1:1,100 DEG C of steam sterilizations 40 minutes.Be 5.8X10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml is with the mixed powder being cooled to 35 DEG C after the bacterium amount that the connects access steam treatment of 2%, and 20 DEG C ferment 45 hours.Then by 5% access
Concentration is 4.5X10 8the aspergillus oryzae spore liquid of cfu/ml, continues fermentation 28 hours at 20 DEG C.
Embodiment 4: by pawpaw: wheat bran: the weight ratio of corn flour=7:1.5:2 prepares 40 object mixed powders.Composite mineral matter solution ratio is lg/LNaN0 3, 3g/LMgS0 47H 20,5g/LK 2hP0 4, 0.6g/LNaCl, 2g/LFeS0 4, 0.8g/LMnS0 4and 0.8g/LCuS0 45H 20, with this composite mineral matter solution humidifying mixed powder, make material-water ratio reach 1:1.2,130 DEG C of steam sterilizations 10 minutes.Be 5.OX10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml is with the mixed powder being cooled to 45 DEG C after the bacterium amount that the connects access steam treatment of 3%, and 30 DEG C ferment 40 hours.Then accessing concentration by 6% is 5.0X10 8the aspergillus oryzae spore liquid of cfu/ml, continues fermentation 50 hours at 25 DEG C.
Preferred embodiment:
The weight ratio of step 1: by pawpaw: wheat bran: corn flour=5.5 6.5:1.5 2.5:1.3 1.7 prepares 60 80 object mixed powders.Step 2: prepare composite mineral matter solution, proportioning is as follows: 1.5 3.5g/LNaN0 3, 0.8 1.8g/LMgS0 4* 7H 20,1.5 3g/LK 2hP0 4, 0.5 1.lg/LNaCl, 1.5 2.2g/LFeS0 4, 0.5 0.7g/LMnS0 4, 0.35 0.45g/LCuS0 45H 20.Step 3: control composite mineral matter liquor capacity and mixed powder mass ratio are 1:0.6 1, damping mixed powder obtains mixture.Step 4: at 100 110 DEG C, steam treatment is carried out 30 40 minutes to mixture.Cell concentration is 3.1 4.0X10 by step 5: after mixture is cooled to 35 45 DEG C 8the saccharomyces cerevisiae nutrient solution of cfu/ml by 4 8% the bacterium amount that connects access, 20 30 DEG C of bottom fermentations 20 40 hours.Step 6: by spore liquid concentration 3.6 4.5X10 8the aspergillus oryzae spore liquid of cfu/ml by 3 5% the bacterium amount that connects access, 22 36 DEG C of bottom fermentations 33 40 hours.
Under this preferred embodiment, final feed product after testing, mixed powder free gossypol content can be down to 130mg/kg, is significantly increased compared with the detoxification efficiency of prior art.
Optimum embodiment:
By pawpaw: wheat bran: the weight ratio of corn flour=7:2:1 prepares 40 object mixed powders, and composite mineral matter solution ratio is 2g/LNaN0 3, lg/LMgS0 47H 20,2g/LK 2hP0 4, lg/LNaCl, 2g/LFeS0 4, 0.6g/LK 2hP0 4and 0.4g/LCuS0 45H 20, with this composite mineral matter solution humidifying mixed powder, make material-water ratio reach 1:0.8, cell concentration is 3.2X10 by 115 DEG C of steam sterilizations 20 minutes 8the saccharomyces cerevisiae nutrient solution of cfu/ml is with the mixed powder being cooled to 40 DEG C after the bacterium amount that the connects access steam treatment of 7%, and 28 DEG C ferment 36 hours, and then accessing concentration by 3% is 4.1X10 8the aspergillus oryzae spore liquid of cfu/ml, 35 DEG C are continued fermentation 24 hours.
Under this optimum embodiment, final feed product after testing, mixed powder free gossypol content can be down to 110mg/kg, and comparatively other embodiment effects of the present invention are particularly outstanding.
The above; be only the present invention's preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.

Claims (6)

1., based on a method for pawpaw reason, change, microbial detoxification technology production animal feed, it is characterized in that, comprise the steps:
51, by pawpaw: wheat bran: the weight ratio of corn flour=57:13:12 prepares 40100 object mixed powders.
2. prepare composite mineral matter solution, proportioning is as follows :
NaN0 315g/L，
MgS0 47H 200.53g/L，
K 2HP0 415g/L，
NaCl0.21.2g/L,
FeS0 40.52.5g/L,
MnS0 40.1lg/L，
CuS0 45H 200.10.8g/L。
3. control composite mineral matter liquor capacity and mixed powder mass ratio are 1:0.41.2, and damping mixed powder obtains mixture.
4. at 80130 DEG C, steam treatment is carried out 1060 minutes to described mixture.
5., after mixture described in is cooled to 1550 DEG C, be 3.05.8X10 by cell concentration 8the saccharomyces cerevisiae nutrient solution of cfu/ml by 210% the bacterium amount that connects access, 1535 DEG C of bottom fermentations 1248 hours.
6. by spore liquid concentration 3.56.OX10 8the aspergillus oryzae spore liquid of cfu/ml by 17% the bacterium amount that connects access, 1540 DEG C of bottom fermentations 2060 hours.
CN201410319530.2A 2014-07-07 2014-07-07 Method for producing animal feed based on detoxifying techniques of heat treatment, chemical treatment and microbiological treatment on papayas Pending CN105265762A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107581348A (en) * 2017-11-06 2018-01-16 李正美 A kind of technology of fresh branches and leaves of peanut seedling Fodder making
US20190069575A1 (en) * 2017-09-01 2019-03-07 Wild Earth, Inc. Food product compositions and methods for producing the same
CN110170019A (en) * 2019-06-05 2019-08-27 赵亚泽 A kind of Chinese medicine and preparation method thereof causing livestock and poultry human body inflammatory reaction for treating virus
US11259546B2 (en) 2017-09-01 2022-03-01 Wild Earth, Inc. Protein-containing compositions

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190069575A1 (en) * 2017-09-01 2019-03-07 Wild Earth, Inc. Food product compositions and methods for producing the same
US10973242B2 (en) 2017-09-01 2021-04-13 Wild Earth, Inc. Protein-containing compositions
US11259546B2 (en) 2017-09-01 2022-03-01 Wild Earth, Inc. Protein-containing compositions
CN107581348A (en) * 2017-11-06 2018-01-16 李正美 A kind of technology of fresh branches and leaves of peanut seedling Fodder making
CN110170019A (en) * 2019-06-05 2019-08-27 赵亚泽 A kind of Chinese medicine and preparation method thereof causing livestock and poultry human body inflammatory reaction for treating virus

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