CN104531552A - Heavy metal contaminated soil microbe repairing agent and application thereof - Google Patents

Heavy metal contaminated soil microbe repairing agent and application thereof Download PDF

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Publication number
CN104531552A
CN104531552A CN201410682558.2A CN201410682558A CN104531552A CN 104531552 A CN104531552 A CN 104531552A CN 201410682558 A CN201410682558 A CN 201410682558A CN 104531552 A CN104531552 A CN 104531552A
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contaminated soil
preserving number
metal contaminated
heavy
heavy metal
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金京勋
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SOOCHOW KH BIO SCIENCE&TECHNOLOGY Co Ltd (KHB)
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SOOCHOW KH BIO SCIENCE&TECHNOLOGY Co Ltd (KHB)
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Soil Sciences (AREA)
  • Molecular Biology (AREA)
  • Processing Of Solid Wastes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a heavy metal contaminated soil microbe repairing agent which comprises the following components: brevibacillus borstelensis, candida tropicalis, nutrients and a carrier. The heavy metal contaminated soil microbe repairing agent is used in the heavy metal contaminated soil repairing microbe, the microbe used in the heavy metal contaminated soil microbe repairing agent can rapidly colonize and grow in soil and form a strong colony, can directly adsorb heavy metal ions into the microbial cell walls or cells for curing and passivation, heavy metal mobility is decreased, and crop heavy metal ion absorption can be reduced. The technology is low in repair cost and convenient in construction, provides a new method for soil microbe repairing, and has good application value.

Description

Heavy-metal contaminated soil microorganism renovation agent and application thereof
Technical field
The present invention relates to a kind of for repairing by heavy metal, the microorganism renovation agent of the soil of especially cadmium pollution, and the application of this microorganism renovation agent in soil remediation.
Background technology
Heavy metal contamination refers to that proportion is greater than the metal of 5 or 4 (mainly comprising Cu, Zn, Cd, Pb, Hg, Cr, As, Ni, Co) or caused the in the environment pollution of its compound.Heavy metal element can be divided into two classes by its biological chemistry matter: a class is the essential element that can maintain living organism normal physiological activity within the scope of finite concentration, if but its concentration exceedes certain limit, and body will be caused poisoning, as copper, zinc etc.; Another kind of, be the nonessential element of organism normal physiological activity, namely harmful element, as cadmium, mercury, lead etc.Cadmium (cd) is the extremely strong heavy metal element of bio-toxicity, is collectively referred to as " five poisonous creatures: scorpion, viper, centipede, house lizard, toad ", comes second with Pb, cr, As and Hg.
Soil is the important component part of environment for human survival, soil energy sticking enters the various pollutents, particularly heavy metal element of its inside, and then accumulates in soil, when semi-invariant exceedes ability to bear and the capacity of permission of soil self, soil pollution will be caused.And heavy metal pollution of soil compares topsoil and water pollution, there is chronicity, disguise, non-reversibility and can not be decomposed completely or the feature such as disappearance, and directly relevant to agriculture production, and therefore harm is serious, improvement is difficult.How effectively to prevent and treat and to solve the attention that heavy metal pollution of soil problem is more and more subject to countries in the world.In recent years, the development of modern industry and the widespread use of cadmium in smelting, chemical industry, papermaking and electronic industry etc., make Cd Pollution in Soil be on the rise, and research finds, the Cd be discharged in environment has 82.94% to enter soil.
Cadmium compounds has more fat-soluble, bioconcentration and toxicity, and being a kind of violent in toxicity, is also one of heavy metal of most mobility in soil.Just priority pollutant is decided to be as far back as United Nations Environment Programme (UNEP)s in 1974 and the international labour hygiene heavy metal council, poisonous substance management committee of the U.S. (ATSDR) is own is classified as the toxic substance that the 6th is jeopardized human health, is defined as the lung cancer of the mankind and the confirmation carcinogens of prostate cancer by international cancer research institution.Cadmium is that body growth grows nonessential element, enters human body on a small quantity namely by biomagnification and biological accumulation, produces a series of damage to kidney, lung, liver, testis, brain, bone and blood system.Eating cause containing excessive cadmium food poisoning is acute mostly, and cardinal symptom is nausea,vomiting,diarrhea, stomachache.The murder by poisoning of cadmium to human body causes the attention of countries in the world, has also occurred the situation of many zone of pollution cadmium poisoning in China.
The grown on soil crop of polluted by heavy metal cadmium, directly can cause grain drop in production, and Cd can make the crude protein in brown rice, Crude starch, amylose starch, Methionin equal size reduce, and reduces the quality of crop.Cd content overproof and close to threshold value in the grain in many places, the food such as vegetables and fruit in addition.The many agricultural-food of China due to cadmium content too high and by refuse outside the gateway of a country of foreign country.How effectively the cadmium pollution in processing environment, reduce the enrichment of cadmium in agricultural-food, become the outstanding problem in environment in recent years protection field.
Bacillus is Gram-positive bacillus, relatively more extensive in distributed in nature, has stronger resistance to poor environment.Have genus bacillus as biological adsorption agent Adsorption of Heavy Metals Pb at present 2+, Cu 2+, Cd 2+and Zn 2+report.In addition, cell walls and other pre biooxidation ratios of genus bacillus, thickness large (20-80nm), chemical composition is simple, the general teichoic acid containing 90% peptidoglycan and 10%.Lot of domestic and international scholar has carried out large quantifier elimination to the mechanism of bacterial adsorption heavy metal, thinks that the adsorption site of gram positive bacterium heavy metal is the carboxyl on whole cell peptidoglycan, teichoic acid and the phosphate on uronic acid.The polymer that bacteria cell wall is formed by connecting by peptidoglycan and teichoic acid forms, and peptidoglycan is the superpolymer that NAM (-acetylmuramic acid) and NAG (2-Acetamido-2-deoxy-D-glucose) replace.These superpolymer are in juxtaposition and are cross-linked, thus form adsorption layer.-acetylmuramic acid is the material being uniquely present in bacteria cell wall, being attached to,-acetylmuramic acid is four short chain amino acid, comprise L-Beta Alanine, D-Beta Alanine, D-Glu and diaminopimelic acid, these short polypeptide chains include carboxyl and amido functional group freely.Another structure of bacteria cell wall is exactly teichoic acid, is made up of the glycerophosphate of short chain, therefore has a large amount of negative charges, this be bacteria cell wall with a large amount of negative charge, the major cause that Adsorption of Heavy Metals ability is strong.Yeast also has the ability of Effective Anti heavy metal, and resistance mechanism and bacterium have very large difference.It has been generally acknowledged that yeast) mainly through utilizing metallothionein(MT) chelating heavy metal of body, to reduce the existence of destructive larger active free state heavy metal.
The advantage of microorganism remediation measure be implement compared with simple, invest less and little to environmental disruption, shortcoming is that regulation effect is not remarkable so far, because microorganism remediation easily by the impact of various environmental factors, as temperature, oxygen, moisture etc. all can affect microorganism active, and then affects repairing effect.
Summary of the invention
The invention provides a kind of heavy-metal contaminated soil microorganism renovation agent, and the application of this microorganism renovation agent in soil remediation, to overcome the above-mentioned defect that prior art exists.
First the present invention is to provide a kind of heavy-metal contaminated soil microorganism renovation agent, and it comprises following component: subtilis, candida tropicalis, nutritive substance and carrier.
The parts by weight of each component are as follows: subtilis 10-30 part, candida tropicalis 5-10 part, nutritive substance 10-30 part and carrier 50-200 part.
Described subtilis is selected from least one bacterial strain in preserving number CMCC63521, preserving number CMCC63522, preserving number CMCC63523, preserving number preserving number CMCC63524, preserving number CMCC63525, preserving number CMCC63526, preserving number CMCC63527, preserving number CMCC63528, preserving number CMCC63529, preserving number CMCC63530;
Described candida tropicalis is selected from least one bacterial strain in preserving number ACCC20004, preserving number ACCC20005, preserving number ACCC20006, preserving number ACCC20148;
Effective colony number of described subtilis is 20-80 × 10 8cfu/g, effective colony number of described candida tropicalis is 30-50 × 10 8cfu/g.
Described nutritive substance is nitrogen, phosphorus, potassium, zinc and iron; The preferred proportioning of each nutritive substance is: described nitrogen, phosphorus, potassium, and the part by weight of zinc and iron is N:P 2o 5: K 2o:ZnO:Fe 2o 3=1.1:1.8:1.5:3:1.
Described carrier is at least one of wilkinite, sepiolite, and preferably adopt the mixture of wilkinite and sepiolite, both weight ratios are 0.3-0.8:1.
As the preferred technical scheme of one, subtilis, candida tropicalis adopt its cultivation rhzomorph obtained after following method grown cultures: the original strain of subtilis and candida tropicalis aseptically carried out respectively successively slant culture, shaking table is cultivated, after fermentor cultivation, the fermentation liquor obtained crossed concentrate drying and obtain described cultivation rhzomorph.
Another object of the present invention is to provide a kind of method preparing described heavy-metal contaminated soil microorganism renovation agent, and it stirs being mixed in proportion by subtilis, candida tropicalis, nutritive substance and carrier and obtains.
A further object of the invention, be to provide in the application of a kind of above-mentioned heavy-metal contaminated soil microorganism renovation agent in heavy-metal contaminated soil is repaired, this heavy-metal contaminated soil microorganism renovation agent is powdery or particulate state formulation, mix thin loess when ploughing soil or mix in base manure and evenly spread fertilizer over the fields, level land, general consumption is 10-80kg/m 2.
Microorganism used in the present invention can rapidly in soil field planting grow and form powerful bacterium colony, energy is Adsorption of Heavy Metal Ions directly, especially solidified in cadmium to a microorganism cells ancient piece of jade, round, flat and with a hole in its centre or cell, passivation, simultaneously to other heavy metal ion in soil, such as chromium, lead etc. also have stronger absorption passivation, stronger comprehensive function reduces the movability of heavy metal ion, reaches the absorption of cutting down crop heavy metal ion.Add nutritive ingredient and can provide energy for microorganism amount reproduction.This technology rehabilitation cost is cheap, and engineering is convenient, provides the novel method that soil microorganisms is repaired, has good application value.
Embodiment
Provide present pre-ferred embodiments below, to describe technical scheme of the present invention in detail.
Embodiment 1
A kind of heavy-metal contaminated soil microorganism renovation agent, it comprises following component:
Subtilis 10 parts,
Candida tropicalis 5 parts,
Nutritive substance 10 parts,
Wilkinite 50 parts.
Described subtilis is the bacterial strain of preserving number CMCC63525;
Described candida tropicalis is at least bacterial strain of preserving number ACCC20004;
Effective colony number of described subtilis is 20 × 10 8cfu/g, effective colony number of described candida tropicalis is 30 × 10 8cfu/g.
Described nutritive substance is nitrogen, phosphorus, potassium, zinc and iron, described nitrogen, phosphorus, potassium, and the part by weight of zinc and iron is N:P 2o 5: K 2o:ZnO:Fe 2o 3=1.1:1.8:1.5:3:1.
Embodiment 2
A kind of heavy-metal contaminated soil microorganism renovation agent, it comprises following component:
Subtilis 30 parts,
Candida tropicalis 10 parts,
Nutritive substance 30 parts,
Sepiolite 200 parts.
Described subtilis is the bacterial strain of preserving number CMCC63521;
Described candida tropicalis is the bacterial strain in preserving number ACCC20148;
Effective colony number of described subtilis is 80 × 10 8cfu/g, effective colony number of described candida tropicalis is 50 × 10 8cfu/g.
Described nutritive substance is nitrogen, phosphorus, potassium, zinc and iron, described nitrogen, phosphorus, potassium, and the part by weight of zinc and iron is N:P 2o 5: K 2o:ZnO:Fe 2o 3=1.1:1.8:1.5:3:1.
Embodiment 3
A kind of heavy-metal contaminated soil microorganism renovation agent, it comprises following component:
Subtilis 20 parts,
Candida tropicalis 80 parts,
Nutritive substance 20 parts,
100 parts, carrier.
Described carrier is the mixture of wilkinite and sepiolite, and both weight ratios are 0.5:1.
Described subtilis is the bacterial strain of preserving number CMCC63522;
Described candida tropicalis is the bacterial strain of preserving number ACCC20006;
Effective colony number of described subtilis is 50 × 10 8cfu/g, effective colony number of described candida tropicalis is 40 × 10 8cfu/g.
Described nutritive substance is nitrogen, phosphorus, potassium, zinc and iron, described nitrogen, phosphorus, potassium, and the part by weight of zinc and iron is N:P 2o 5: K 2o:ZnO:Fe 2o 3=1.1:1.8:1.5:3:1.
Experimental example
The air-dry soil crossing 3mm sieve is prepared into the cadmium pollution soil of 2mg/kg, after abundant mixing, load in the plastic flowerpot of diameter 10cm, high 10cm, every basin fills native 500g, adding deionized water makes water content be 60% of field capacity, evenly sowed in soil by the microorganism renovation agent of embodiment 1-3 respectively, consumption is 20kg/m 2.Put in vegetation box and cultivate, impose a condition for: day temperature is 25 DEG C, full exposure; Evening, temperature was 18 DEG C, dark; Whole day humidity is 75%.Within 30 days, distinguish collection surface, middle part and bottom pedotheque afterwards, Cadmium in Soil adopts CaCl 2lixiviate-atomic absorption spectrophotometry (graphite furnace) measures.
Table 1 each sample Cadmium in Soil content (mg/kg)
Surface soil Middle part soil Bottom soil
Embodiment 1 0.103 0.132 0.176
Embodiment 2 0.096 0.115 0.154
Embodiment 3 0.057 0.071 0.092
As can be seen from experimental result, microorganism renovation agent of the present invention can significantly reduce Cadmium in Soil content, the soil inside and effect can be gone directly.

Claims (9)

1. a heavy-metal contaminated soil microorganism renovation agent, is characterized in that, comprises following component: subtilis, candida tropicalis, nutritive substance and carrier.
2. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 1, it is characterized in that, the parts by weight of each component are as follows: subtilis 10-30 part, candida tropicalis 5-10 part, nutritive substance 10-30 part and carrier 50-200 part.
3. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 1, it is characterized in that, described subtilis is selected from least one bacterial strain in preserving number CMCC63521, preserving number CMCC63522, preserving number CMCC63523, preserving number preserving number CMCC63524, preserving number CMCC63525, preserving number CMCC63526, preserving number CMCC63527, preserving number CMCC63528, preserving number CMCC63529, preserving number CMCC63530; Described candida tropicalis is selected from least one bacterial strain in preserving number ACCC20004, preserving number ACCC20005, preserving number ACCC20006, preserving number ACCC20148.
4. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 1, it is characterized in that, effective colony number of described subtilis is 20-80 × 10 8cfu/g, effective colony number of described candida tropicalis is 30-50 × 10 8cfu/g.
5. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 1, it is characterized in that, described nutritive substance is nitrogen, phosphorus, potassium, zinc and iron.
6. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 5, it is characterized in that, described nitrogen, phosphorus, potassium, the part by weight of zinc and iron is N:P 2o 5: K 2o:ZnO:Fe 2o 3=1.1:1.8:1.5:3:1.
7. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 1, it is characterized in that, described carrier is at least one of wilkinite, sepiolite.
8. heavy-metal contaminated soil microorganism renovation agent as claimed in claim 1, it is characterized in that, described carrier is the mixture of wilkinite and sepiolite, and both weight ratios are 0.3-0.8:1.
9. the application of heavy-metal contaminated soil microorganism renovation agent in heavy-metal contaminated soil is repaired as described in any one of claim 1-8.
CN201410682558.2A 2014-11-24 2014-11-24 Heavy metal contaminated soil microbe repairing agent and application thereof Pending CN104531552A (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154374A (en) * 2015-10-14 2015-12-16 武汉瑞泽园生物环保科技有限公司 Heavy metal passivation microbial inoculum and preparation method thereof
CN105234168A (en) * 2015-11-20 2016-01-13 福建康德贝生物科技有限公司 Application of bacillus subtilis
CN106607454A (en) * 2016-12-20 2017-05-03 平顶山学院 Remediation method for chrome-polluted soil in coal mine collapse region
CN106734183A (en) * 2016-12-20 2017-05-31 平顶山学院 A kind of mining area nickel contamination soil remediation method
CN106964647A (en) * 2017-06-06 2017-07-21 临沂大学 A kind of method of utilization composite bacteria agent remediating lead-contaminated soil
CN107418913A (en) * 2017-06-14 2017-12-01 湖南省农业生物技术研究中心 A kind of application of the microorganism formulation of Efficient Conversion Heavy Metals in Contaminated Soils cadmium
CN108637000A (en) * 2016-05-12 2018-10-12 温州泓呈祥科技有限公司 Soil remediation device for removing organochlorine pesticide components in farmland soil
CN111014280A (en) * 2019-12-27 2020-04-17 武汉理工大学 Method for repairing cadmium-polluted soil by using bacteria-montmorillonite compound
CN114105315A (en) * 2021-12-09 2022-03-01 延边大学 Method for adsorbing and extracting heavy metals in heavy metal pollutants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ABDUL REHMAN，ET AL: "Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents: glutathione as detoxifying agent", 《ENVIRON MONIT ASSESS》 *
W.BAHAFID，ET AL: "Bioaugmentation of Chromium-Polluted Soil Microcosms with Candida Tropicalis Diminishes Phytoavailable Chromium", 《JOURNAL OF APPLIED MICROBIOLOGY》 *
李晓晴: "枯草芽孢杆菌对花生镉积累的影响机制研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154374A (en) * 2015-10-14 2015-12-16 武汉瑞泽园生物环保科技有限公司 Heavy metal passivation microbial inoculum and preparation method thereof
CN105154374B (en) * 2015-10-14 2018-06-29 武汉瑞泽园生物环保科技有限公司 Heavy metal passivation microbial inoculum and preparation method thereof
CN105234168A (en) * 2015-11-20 2016-01-13 福建康德贝生物科技有限公司 Application of bacillus subtilis
CN108637000A (en) * 2016-05-12 2018-10-12 温州泓呈祥科技有限公司 Soil remediation device for removing organochlorine pesticide components in farmland soil
CN106607454A (en) * 2016-12-20 2017-05-03 平顶山学院 Remediation method for chrome-polluted soil in coal mine collapse region
CN106734183A (en) * 2016-12-20 2017-05-31 平顶山学院 A kind of mining area nickel contamination soil remediation method
CN106734183B (en) * 2016-12-20 2019-09-27 平顶山学院 A kind of mining area nickel contamination soil remediation method
CN106964647A (en) * 2017-06-06 2017-07-21 临沂大学 A kind of method of utilization composite bacteria agent remediating lead-contaminated soil
CN107418913A (en) * 2017-06-14 2017-12-01 湖南省农业生物技术研究中心 A kind of application of the microorganism formulation of Efficient Conversion Heavy Metals in Contaminated Soils cadmium
CN111014280A (en) * 2019-12-27 2020-04-17 武汉理工大学 Method for repairing cadmium-polluted soil by using bacteria-montmorillonite compound
CN114105315A (en) * 2021-12-09 2022-03-01 延边大学 Method for adsorbing and extracting heavy metals in heavy metal pollutants
CN114105315B (en) * 2021-12-09 2023-11-14 延边大学 Method for adsorbing and extracting heavy metals in heavy metal pollutants

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Application publication date: 20150422