CN105154374B - Heavy metal passivation microbial inoculum and preparation method thereof - Google Patents
Heavy metal passivation microbial inoculum and preparation method thereof Download PDFInfo
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Abstract
The present invention provides heavy metal species passivation microbial inoculum, includes the raw material of following parts by weight：40 50 parts of 50 60 parts of active ingredient and auxiliary material；The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the weight ratio of the pseudomonas putida, the saccharomycete and the bacillus subtilis is 1:0.8‑1.2:2.5‑3.5；The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, the wheat bran, the brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 35 45:8‑10:1:55‑65:55‑65.The present invention also provides the preparation methods of heavy metal passivation microbial inoculum.The present invention has better effects to various heavy in passivation soil, and the heavy metal in soil can be changed into low toxicity or nontoxic form.
Description
Technical field
The present invention relates to a kind of microbial inoculums of heavy metal that can be passivated in soil and preparation method thereof.
Background technology
Heavy metal such as cadmium, arsenic in soil can not natural decomposition, these heavy metals can influence the yield and quality of crops,
The serious destruction of the ecosystem is caused, the health of the mankind can be especially endangered by food chain.Changed using microbial bacterial agent
Good soil texture has proven to a kind of effective method, but this microbial inoculum cannot generally be passivated the huge sum of money in soil
Belong to.Therefore, heavy metal species passivation microbial inoculum can be designed, the heavy metal being passivated in soil reduces crops to these heavy metals
Absorptivity, the final content for reducing heavy metal in crops.
Invention content
The object of the present invention is to provide a heavy metal species to be passivated microbial inoculum, has to the various heavy in soil preferably blunt
Change effect, heavy metal in soil can be changed into low toxicity or nontoxic form.
A further object of the invention is that the preparation method of microbial inoculum is passivated by a heavy metal species, is capable of providing heavy metal
The passivation ability and passivation efficiency of microbial inoculum are passivated, reduces the dosage of passivation microbial inoculum.
In order to realize these purposes and other advantages according to the present invention, heavy metal species passivation microbial inoculum is provided, including
The raw material of following parts by weight：
40-50 parts of 50-60 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:0.8-1.2:2.5-3.5；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 35-45:8-10:1:55-65:55-
65。
Preferably, heavy metal passivation microbial inoculum, the pseudomonas putida, the saccharomycete and the withered grass bud
The weight ratio of spore bacillus is 1:1:3, the wheat bran, the brown sugar, the potassium dihydrogen phosphate, the Paris white and the husk
The weight ratio of powder is 40:9:1:60:60.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
Preferably, the preparation method of described heavy metal passivation microbial inoculum, in addition pseudomonas putida, saccharomycete and withered
After careless bacillus, the additive of 25-30 parts of addition；
The additive includes magnetite powder, energy stone powder and Powdered Activated Carbon, wherein, it is the magnetite powder, described
The weight ratio of energy stone powder and the Powdered Activated Carbon is 1:1:1.
Preferably, the preparation method of heavy metal passivation microbial inoculum, further includes：
Mixture equivalent after crushing is divided into three parts, is then handled 30-40 minutes with special device；
The hollow columnar structures that the special device is vertically arranged, and it is closed at both ends, on the special device side wall
Portion is set there are three feed inlet, which is located at same level, and each feed inlet is communicated with a feed pipe, Mei Gejin
Expects pipe is set with vertical direction in 45° angle, which is respectively used to the mixture after first part of addition crushes, second
Mixture after mixture and third part crush after part crushes；Be equipped with an air inlet in the middle part of the special device side wall, it is described into
Air port is connected with a blast pipe, and the blast pipe is set along the tangent to periphery direction of the special device, and the blast pipe is also
It is connected with an air blower, for the air blast into the special device；The special device side wall center outer surface is equipped with a ultrasound
Wave producer, for the ultrasonic wave of special device internal irradiation 20MHz；Special device outer surface lower part is additionally provided with one and goes out
Material mouth.
Preferably, the preparation method of heavy metal passivation microbial inoculum, the internal diameter of the special device is 0.8 meter.
The present invention includes at least following advantageous effect：
(1) composite bacteria agent of the invention has better effects to various heavy in passivation soil, can be by heavy metal in soil
It is changed into low toxicity or nontoxic form.The effect of wherein composite bacteria agent passivation cadmium is especially notable, and the removal rate of heavy metal cadmium reaches
87.63%.Wherein, pseudomonas putida is mainly passivated cadmium element, saccharomycete oxidized portion heavy metal element, bacillus subtilis
Bacterium being capable of absorbed portion heavy metal element.
(2) present invention is added with the additive that composition is magnetite powder, energy stone powder and Powdered Activated Carbon, first magnetite
Powder and energy stone powder are respectively provided with certain magnetism, after the heavy metal passivation microbial inoculum of the present invention is sprayed onto in soil, due to
Attracting each other between magnetite powder and energy stone powder can enable active ingredient and auxiliary material long period be attached on soil simultaneously
To be fully passivated to the heavy metal in soil on particle, which improves the present invention passivation ability and passivation effect,
Secondary, Powdered Activated Carbon has stronger adsorption capacity, can be by a certain amount of active ingredient and auxiliary material absorption around it, activity
There is more auxiliary material around composition, this just makes there are more nutriment around active ingredient, so as to very high survival
Rate, thus the usage amount of fermenting agent can be reduced, it is cost-effective.In the present invention, the dosage of additive and active ingredient
Ratio is preferably 1:2, when ratio is less than 1:When 2, passivation ability of the invention and passivation effect are substantially reduced.
(3) special device of the invention is used to strengthen the mixability of each component in raw material, and additive is enable to be passivated
It is uniformly distributed in microbial inoculum, utmostly plays the effect of additive.Mixture equivalent is divided into three parts first, respectively from three into
It being added in special device in expects pipe, mixture encounters the spiralling air-flow blasted in blast pipe during whereabouts, with
It moves, and under the action of ultrasonic wave, accelerates with flow spiral, each component in such raw material can be sufficiently mixed.
Under the processing of special device, additive can be evenly distributed in passivation microbial inoculum, and then is played during heavy metal-passivated
The passivation ability and passivation effect of passivation microbial inoculum is greatly improved in great function.It is 30- using the preferred time that special device is handled
40 minutes, more than 40 minutes, ultrasonic wave can destroy active ingredient.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is the structure diagram of the special device of the present invention.
Specific embodiment
With reference to example, the present invention is described in further detail, to enable those skilled in the art with reference to specification text
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other element or combinations.
<Example 1>
One heavy metal species are passivated microbial inoculum, include the raw material of following parts by weight：
45 parts of 55 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:1:3；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 40:9:1:60:60.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
<Example 2>
One heavy metal species are passivated microbial inoculum, include the raw material of following parts by weight：
50 parts of 50 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:0.8:2.5；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 35:8:1:55:55.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
<Example 3>
One heavy metal species are passivated microbial inoculum, include the raw material of following parts by weight：
40 parts of 60 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:1.2:3.5；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 45:10:1:65:65.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
<Example 4>
One heavy metal species are passivated microbial inoculum, include the raw material of following parts by weight：
45 parts of 55 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:1:3；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 40:9:1:60:60.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
After addition pseudomonas putida, saccharomycete and bacillus subtilis, the additive of 27.5 parts of addition；
The additive includes magnetite powder, energy stone powder and Powdered Activated Carbon, wherein, it is the magnetite powder, described
The weight ratio of energy stone powder and the Powdered Activated Carbon is 1:1:1.
The preparation method of the heavy metal passivation microbial inoculum, further includes：
Mixture equivalent after crushing is divided into three parts, is then handled 35 minutes with special device；
As shown in Figure 1, the hollow columnar structures that the special device is vertically arranged, and it is closed at both ends, it is described special
Device side wall upper part is set there are three feed inlet, which is located at same level, each feed inlet be communicated with one into
Expects pipe 1, each feed pipe 1 are set with vertical direction in 45° angle, which is respectively used to add in first part of crushing
Rear mixture, second part crush after mixture and third part crush after mixture；It is equipped in the middle part of the special device side wall
One air inlet, the air inlet are connected with a blast pipe 2, the blast pipe 2 along the special device tangent to periphery direction
Setting, the blast pipe 2 is also connected with an air blower, for the air blast into the special device；In the special device side wall
Portion outer surface is equipped with a supersonic generator 3, for the ultrasonic wave of special device internal irradiation 20MHz；The special device
Outer surface lower part is additionally provided with a discharge port.
The internal diameter of the special device is 0.8 meter.
<Example 5>
One heavy metal species are passivated microbial inoculum, include the raw material of following parts by weight：
50 parts of 50 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:0.8:2.5；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 35:8:1:55:55.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
After addition pseudomonas putida, saccharomycete and bacillus subtilis, the additive of 25 parts of addition；
The additive includes magnetite powder, energy stone powder and Powdered Activated Carbon, wherein, it is the magnetite powder, described
The weight ratio of energy stone powder and the Powdered Activated Carbon is 1:1:1.
The preparation method of the heavy metal passivation microbial inoculum, further includes：
Mixture equivalent after crushing is divided into three parts, is then handled 30 minutes with special device；
The hollow columnar structures that the special device is vertically arranged, and it is closed at both ends, on the special device side wall
Portion is set there are three feed inlet, which is located at same level, and each feed inlet is communicated with a feed pipe, Mei Gejin
Expects pipe is set with vertical direction in 45° angle, which is respectively used to the mixture after first part of addition crushes, second
Mixture after mixture and third part crush after part crushes；Be equipped with an air inlet in the middle part of the special device side wall, it is described into
Air port is connected with a blast pipe, and the blast pipe is set along the tangent to periphery direction of the special device, and the blast pipe is also
It is connected with an air blower, for the air blast into the special device；The special device side wall center outer surface is equipped with a ultrasound
Wave producer, for the ultrasonic wave of special device internal irradiation 20MHz；Special device outer surface lower part is additionally provided with one and goes out
Material mouth.
The internal diameter of the special device is 0.8 meter.
<Example 6>
One heavy metal species are passivated microbial inoculum, include the raw material of following parts by weight：
40 parts of 60 parts of active ingredient and auxiliary material；
The active ingredient includes pseudomonas putida, saccharomycete, bacillus subtilis, wherein, the Pseudomonas putida
The weight ratio of bacterium, the saccharomycete and the bacillus subtilis is 1:1.2:3.5；
The auxiliary material includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, it is the wheat bran, described
Brown sugar, the potassium dihydrogen phosphate, the Paris white and the rice husk powder weight ratio be 45:10:1:65:65.
One heavy metal species are passivated the preparation method of microbial inoculum, including：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned heavy
Amount is uniformly mixed than addition pseudomonas putida, saccharomycete, bacillus subtilis, obtains mixture, crush blunt to get heavy metal
Change microbial inoculum.
After addition pseudomonas putida, saccharomycete and bacillus subtilis, the additive of 30 parts of addition；
The additive includes magnetite powder, energy stone powder and Powdered Activated Carbon, wherein, it is the magnetite powder, described
The weight ratio of energy stone powder and the Powdered Activated Carbon is 1:1:1.
The preparation method of the heavy metal passivation microbial inoculum, further includes：
Mixture equivalent after crushing is divided into three parts, is then handled 40 minutes with special device；
The hollow columnar structures that the special device is vertically arranged, and it is closed at both ends, on the special device side wall
Portion is set there are three feed inlet, which is located at same level, and each feed inlet is communicated with a feed pipe, Mei Gejin
Expects pipe is set with vertical direction in 45° angle, which is respectively used to the mixture after first part of addition crushes, second
Mixture after mixture and third part crush after part crushes；Be equipped with an air inlet in the middle part of the special device side wall, it is described into
Air port is connected with a blast pipe, and the blast pipe is set along the tangent to periphery direction of the special device, and the blast pipe is also
It is connected with an air blower, for the air blast into the special device；The special device side wall center outer surface is equipped with a ultrasound
Wave producer, for the ultrasonic wave of special device internal irradiation 20MHz；Special device outer surface lower part is additionally provided with one and goes out
Material mouth.
The internal diameter of the special device is 0.8 meter.
In order to illustrate the effect of the present invention, it is as follows that inventor provides comparative experiments：
<Comparative example 1>
During heavy metal passivation microbial inoculum is prepared, additive is not added, remaining parameter and the complete phase in example 4
Together, technical process is also identical.
<Comparative example 2>
During heavy metal passivation microbial inoculum is prepared, the mixture after crushing is not handled with special device, remaining ginseng
For number with identical in example 4, technical process is also identical.
The arable land of one piece of heavy metal pollution is chosen, 9 pieces of test areas is divided into, any instance and comparative example is obtained
Heavy metal passivation microbial inoculum is applied to one piece of test area and is tested, the amount phase of passivation microbial inoculum that every piece of test area applies
Deng, last block test area only using the auxiliary material of equivalent as blank group.The exchangeable species of cadmium, lead and copper in arable soil
Content is respectively 1.80 μ g/g, 40 μ g/g and 15 μ g/g, after four months, chooses soil sample from each test area, measures in soil sample
Exchangeable species content of beary metal, obtains that the results are shown in Table 1.
[the heavy metal passivation microbial inoculum that 1 each example of table and comparative example obtain is to the disposition of each test area soil]
| Cadmium (μ g/g) | Lead (μ g/g) | Copper (μ g/g) | |
| Example 1 | 1.61 | 38.5 | 9.50 |
| Example 2 | 1.60 | 38.2 | 9.57 |
| Example 3 | 1.63 | 38.1 | 9.59 |
| Example 4 | 1.42 | 36.5 | 9.32 |
| Example 5 | 1.45 | 36.9 | 9.37 |
| Example 6 | 1.45 | 37.0 | 9.33 |
| Comparative example 1 | 1.59 | 37.7 | 9.48 |
| Comparative example 2 | 1.60 | 37.9 | 9.48 |
| Blank group | 1.80 | 40.0 | 14.90 |
From upper table 1, it can be seen that the heavy metal in soil is difficult natural decomposition, it is necessary to use matal deactivator.It is and real
Due to during heavy metal passivation microbial inoculum is prepared, being added to additive in example 4, obtained heavy metal passivation microbial inoculum pair
The removal rate of cadmium, lead and copper is significantly higher than the situation of comparative example 1, shows that the passivation ability of heavy metal passivation microbial inoculum is significantly higher than ratio
Compared with example 1.In example 4 due to prepare heavy metal passivation microbial inoculum during, due to by crush after mixture special device
Processing obtains heavy metal and is passivated microbial inoculum to the situation of a little higher than comparative example of the removal rate of cadmium, lead and copper 2, shows that heavy metal is blunt
The passivation ability for changing microbial inoculum is higher than comparative example 2.As it can be seen that the present invention, during heavy metal passivation microbial inoculum is prepared, addition is added
Agent and the passivation ability that heavy metal passivation microbial inoculum can be significantly improved with the mixture that special device is handled after crushing.
Although the embodiments of the present invention have been disclosed as above, but its be not restricted in specification and embodiment it is listed
With.It can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily
Realize other modification.Therefore without departing from the general concept defined in the claims and the equivalent scope, it is of the invention and unlimited
In specific details and example shown and described herein.
Claims (3)
1. a heavy metal species are passivated the preparation method of microbial inoculum, which is characterized in that heavy metal passivation microbial inoculum includes following parts by weight
Raw material：40-50 parts of 50-60 parts of active ingredient and auxiliary material；The active ingredient includes pseudomonas putida, saccharomycete, withered grass
Bacillus, wherein, the weight ratio of the pseudomonas putida, the saccharomycete and the bacillus subtilis is 1:1:3；Institute
It states auxiliary material and includes wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder, wherein, the wheat bran, the brown sugar, the phosphorus
The weight ratio of acid dihydride potassium, the Paris white and the rice husk powder is 40:9:1:60:60；
Preparation method includes：
Wheat bran, brown sugar, potassium dihydrogen phosphate, Paris white and rice husk powder are taken according to above-mentioned weight ratio, then according to above-mentioned weight ratio
Pseudomonas putida, saccharomycete, bacillus subtilis are added, is uniformly mixed, obtains mixture, crushes and is passivated bacterium to get heavy metal
Agent；After addition pseudomonas putida, saccharomycete and bacillus subtilis, the additive of 25-30 parts of addition；
The additive includes magnetite powder, energy stone powder and Powdered Activated Carbon, wherein, the magnetite powder, the energy
The weight ratio of stone powder and the Powdered Activated Carbon is 1:1:1.
2. the preparation method of heavy metal passivation microbial inoculum as described in claim 1, which is characterized in that further include：
Mixture equivalent after crushing is divided into three parts, is then handled 30-40 minutes with special device；
The hollow columnar structures that the special device is vertically arranged, and it is closed at both ends, the special device side wall upper part is set
There are three feed inlet, which is located at same level, and each feed inlet is communicated with a feed pipe, each feed pipe
It is set with vertical direction in 45° angle, which is respectively used to the mixture after first part of addition crushes, second part of powder
Mixture after mixture and third part crush after broken；An air inlet, the air inlet are equipped in the middle part of the special device side wall
It is connected with a blast pipe, the blast pipe is set along the tangent to periphery direction of the special device, and the blast pipe is also with one
Air blower connects, for the air blast into the special device；The special device side wall center outer surface is sent out equipped with a ultrasonic wave
Raw device, for the ultrasonic wave of special device internal irradiation 20MHz；Special device outer surface lower part is additionally provided with a discharging
Mouthful.
3. the preparation method of heavy metal passivation microbial inoculum as claimed in claim 2, which is characterized in that the internal diameter of the special device
It is 0.8 meter.
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| CN105964666B (en) * | 2016-05-24 | 2019-12-10 | 北京建工环境修复股份有限公司 | Soil remediation method |
| CN107602080A (en) * | 2017-10-31 | 2018-01-19 | 广西旭腾实业集团有限公司 | Based on graphene and stone material mine tailing foamed light high temperature resistant wallboard and preparation method thereof |
| CN108998041A (en) * | 2018-06-01 | 2018-12-14 | 王玲 | A kind for the treatment of agent and preparation method thereof for heavy-metal contaminated soil |
| CN110468080B (en) * | 2019-07-19 | 2024-01-19 | 湖南农业大学 | Microbial agent for promoting rice growth and reducing cadmium as well as preparation method and application method thereof |
| CN110698269A (en) * | 2019-11-05 | 2020-01-17 | 浙江科技学院 | Slow-release type farmland soil heavy metal passivation biochar and preparation method thereof |
| CN110885176A (en) * | 2019-12-11 | 2020-03-17 | 河南莫大生物科技有限公司 | Method for treating and disposing sludge by utilizing microbial flocculant and microbial wall breaking |
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