CN103361072A - Cadmium pollution bioremediation agent as well as preparation method and application thereof - Google Patents
Cadmium pollution bioremediation agent as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103361072A CN103361072A CN201310289456XA CN201310289456A CN103361072A CN 103361072 A CN103361072 A CN 103361072A CN 201310289456X A CN201310289456X A CN 201310289456XA CN 201310289456 A CN201310289456 A CN 201310289456A CN 103361072 A CN103361072 A CN 103361072A
- Authority
- CN
- China
- Prior art keywords
- cadmium
- rhzomorph
- cadmium pollution
- bioremediation agents
- core
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 124
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 122
- 238000002360 preparation method Methods 0.000 title claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 62
- 108090000790 Enzymes Proteins 0.000 claims abstract description 42
- 102000004190 Enzymes Human genes 0.000 claims abstract description 42
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 24
- 239000004615 ingredient Substances 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims description 30
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 22
- 239000011149 active material Substances 0.000 claims description 21
- 230000000050 nutritive effect Effects 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 102000004169 proteins and genes Human genes 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 7
- 229910052698 phosphorus Inorganic materials 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 5
- 229910052742 iron Inorganic materials 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 239000011574 phosphorus Substances 0.000 claims description 5
- 239000011591 potassium Substances 0.000 claims description 5
- 229910052725 zinc Inorganic materials 0.000 claims description 5
- 239000011701 zinc Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000004113 Sepiolite Substances 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical group O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 3
- 235000019355 sepiolite Nutrition 0.000 claims description 3
- 229910052624 sepiolite Inorganic materials 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 51
- 239000002689 soil Substances 0.000 abstract description 40
- 150000002500 ions Chemical class 0.000 abstract description 18
- 238000010521 absorption reaction Methods 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 12
- 238000002161 passivation Methods 0.000 abstract description 9
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- 244000005700 microbiome Species 0.000 abstract description 6
- 239000011651 chromium Substances 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 3
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052804 chromium Inorganic materials 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- -1 cadmium Cd<+1> Chemical class 0.000 abstract 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 19
- 235000007164 Oryza sativa Nutrition 0.000 description 19
- 235000009566 rice Nutrition 0.000 description 19
- 235000021329 brown rice Nutrition 0.000 description 18
- 238000012360 testing method Methods 0.000 description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 239000010902 straw Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000000287 crude extract Substances 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 229910002804 graphite Inorganic materials 0.000 description 3
- 239000010439 graphite Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 150000001661 cadmium Chemical class 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000010977 jade Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 229910017604 nitric acid Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000015921 sodium selenite Nutrition 0.000 description 2
- 239000011781 sodium selenite Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- YZCKVEUIGOORGS-UHFFFAOYSA-N Hydrogen atom Chemical compound [H] YZCKVEUIGOORGS-UHFFFAOYSA-N 0.000 description 1
- 229910003424 Na2SeO3 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 208000005882 cadmium poisoning Diseases 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 229960003280 cupric chloride Drugs 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- 150000004686 pentahydrates Chemical class 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
Landscapes
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a cadmium pollution bioremediation agent which is composed of the following ingredients in parts by weight: 10-20 parts of core rhzomorph, 10-20 parts of a nutritional ingredient, 2-6 parts of an enzyme activity substance and 54-78 parts of an rhzomorph carrier. The invention further provides an application of the cadmium pollution bioremediation agent. The cadmium pollution bioremediation agent is reasonable in microorganism specie collocation and high in comprehensive function. Microorganisms in the core rhzomorph can be planted and grow in soil rapidly to form strong bacterial colonies, and heavy metal ions, namely, cadmium Cd<+1> can be directly absorbed to microbial cell walls or into cells to be cured and passivated. Besides, bacillus cereus and beer yeast in the composition have high absorption and passivation function on heavy metal ions, namely, cadmium Cd<+1>, chromium Cr<+1> and lead Pb<+1>, due to the high comprehensive function, the mobility of the heavy metal ions, namely, cadmium Cd<+1>, is reduced, and the purpose of reducing absorption of crops on the heavy metal ions is achieved.
Description
Technical field
The invention belongs to agriculture microbe application field, be specifically related to bioremediation agents that contains active bacterium and its preparation method and application.
Background technology
Along with industrialization and intensive agriculture expanding economy, China's arable land Heavy Metal Pollution is more and more serious.Early 1990s, China's cadmium pollution cultivated area reaches 1.3 * 104/hm
2, relate to 25 areas of 11 provinces and cities.Show according to Hunan Province's heavy metal contamination ad hoc survey and agricultural environment quality monitoring result in recent years, oneself accounts for 23.7 of the whole province total cultivated area the contaminated cultivated area in Hunan Province, about 27% farmland irrigating water and about 25% farmland atmosphere have been subject to pollution in various degree in addition, and principal pollutant are the heavy metal elements such as cadmium, lead.For example zhuzhou, hunan province city in January, 2006, the cadmium pollution event that the Singapore and Malaysia village reverberates throughout the nation has 2 people dead because of unknown cause, and 150 villagers are judged as the chronic mild cadmium poisoning through health check-up; The heavy metal wastewater thereby that other has thousands of mu of soils, village such as new bridge, Xia Wan and construction of being positioned at edge, Xia Wan manufacturing district just to be discharged by the Xia Wan manufacturing district before the eighties in last century pollutes.Local government provides the subsidy of 800 jin of rice every year to every mu of rice field, provided more than 20 year.Paddy rice accumulates the absorption of cadmium on the heavy-metal contaminated soil in order to reduce, and has mainly studied at present dual mode both at home and abroad: the one, the cadmium in the soil is converted into the indissoluble attitude, and namely the activated state cadmium in the soil is passivated, thereby makes crop be difficult to absorb; The 2nd, cadmium is removed from soil, namely utilize engineering or biological method cadmium pollution soil repair.To repair required fund larger because of engineering for the latter, is difficult to carry out spread.Therefore, research how to reduce with the passivation fixing soil in the method for the heavy metals such as cadmium, be of great immediate significance.Lay the foundation for rationally instructing the rice cadmium pollution to subdue, the great special project of Hunan Province in 2012 science and technology " the rice cadmium pollution is subdued and Fast Detection Technique is studied with equipment " starts in the Changsha, Hunan.Special project is carried out the whole province's paddy soil cadmium content distribution situation, the low research that absorbs Selection By Sifting of Rice Varieties, the passivation of paddy soil cadmium and farming technique and rice cadmium Fast Detection Technique and equipment of cadmium, subdue and blocking-up to the cadmium pollution of dining table whole process in the realization farmland, and the whole province's rice safety in production and regulatory format are set up in guiding.Domestic application microorganism repairing heavy metal in soil pollutes existing many reports, also has the scholar to think that biological restoration is difficult to reach in a short time safety standards for the mild or moderate pollution level.For this reason, the present invention adopts many bacterial classifications efficiently to make up, and puts in the soil and can form rapidly powerful bacterium colony, Adsorption of Heavy Metal Ions cadmium Cd
+Solidify in a microorganism cells ancient piece of jade, round, flat and with a hole in its centre or the cell, passivation 2007.34(4) .659～662. microbiologies circular. Rhodopseudomonas palustris is removed the research of cadmium. Bai Hongjuan. Zhang Zhaoming etc. ］, and the derivative that produces by its reproductive process is to heavy metal ion cadmium Cd
+Absorption, passivation, the acting in conjunction of chelating, thereby reduce heavy metal ion cadmium Cd
+Movability, realize to cut down crop to heavy metal ion cadmium Cd
+Absorption.
Summary of the invention
For the weak point of this area, realize cutting down crop to heavy metal ion cadmium Cd
+Absorption, the invention provides a kind of cadmium pollution bioremediation agents.
Another object of the present invention provides the preparation method of cadmium pollution bioremediation agents.
The 3rd purpose of the present invention provides the application of cadmium pollution bioremediation agents.
The concrete technical scheme that realizes above-mentioned purpose of the present invention is:
A kind of cadmium pollution bioremediation agents, described cadmium pollution bioremediation agents is grouped into by following one-tenth by weight:
Core rhzomorph 10-20 part;
Nutritive ingredient 10-20 part;
Enzyme active material 2-6 part;
Rhzomorph carrier 54-78 part;
Wherein, described core rhzomorph is at least a among Rhodopseudomonas palustris (Rhodop seudanonas palustris) preserving number ACCC10649, preserving number ACCC00309, the preserving number ACCC00311, bacillus cereus (Bacillus cereus) number of containing ACCC02803, at least a combination among cereuisiae fermentum (Sacchaeomyces cerevisiae) preserving number ACCC21144, the preserving number ACCC21139.Described bacterial classification is all available from Chinese agriculture microbial strains preservation administrative center.
Preferably, described cadmium pollution bioremediation agents is grouped into by following one-tenth by weight:
15 parts of core rhzomorphs;
15 parts of nutritive ingredients;
4 parts of enzyme active materials;
66 parts in rhzomorph carrier.
Wherein, the core rhzomorph in the described cadmium pollution bioremediation agents comprises Rhodopseudomonas palustris 30.0～50.0 * 10
8Cfu/g, bacillus cereus 4.0～16.0 * 10
8Cfu/g, cereuisiae fermentum 4.0～16.0 * 10
8Cfu/g, total living bacteria count 〉=60.0 * 10
8Cfu/g; Be preferably Rhodopseudomonas palustris 40.0 * 10
8Cfu/g, bacillus cereus 10.0 * 10
8Cfu/g, cereuisiae fermentum 10.0 * 10
8Cfu/g, total living bacteria count 〉=60.0 * 10
8Cfu/g.
Wherein, described nutritive ingredient is nitrogen, phosphorus, potassium, and zinc and iron; Described nitrogen, phosphorus, potassium, and the part by weight of zinc and iron is N:P
2O
5: K
2O:ZnO:Fe
2O
3=1.3:1.7:2:4:1.
Wherein, described enzyme active material is protein powder and organized enzyme.Described enzyme active material is according to protein powder: organized enzyme is that the part by weight of 300:1～100:1 evenly is mixed; Preferably described enzyme active material is according to protein powder: organized enzyme is that the part by weight of 200:1 evenly is mixed.Described organized enzyme is the organized enzyme that extracts from large pea green pod.The method of extracting is: at first the first-class good blue or green pod of soybean is carried out the fragmentation of plant tissue cell.Adopt phosphoric acid buffer (pH value 6.0) to be mixed in the vessel according to solid-liquid ratio 1:5, the mixed solution that obtains filters (filtering 2～3 times), removes solid impurity, obtains the crude extract of enzyme.Then 58 ℃ of heating in water bath of crude extract to obtaining, take out vessel after 10 minutes, leave standstill 5 minutes (must guard against long), the vessel bottom settlings goes out in the large pea green folder to contain solid impurity protein, get supernatant liquor in another vessel, precipitate (pH value 5.8) with ammonium sulfate.Use at last phosphoric acid buffer (pH value 5.7) that the enzyme liquid that resolution of precipitate obtains is carried out separation and purification.
Wherein, described rhzomorph carrier is wilkinite: sepiolite powder evenly mixes in the ratio of 1:0.8～1.5; Described cadmium pollution bioremediation agents is pulvis or granule,
The preparation method of cadmium pollution bioremediation agents of the present invention, it is with by weight 10-20 part core rhzomorph, 10-20 part nutritive ingredient, 2-6 part enzyme active material, 54-78 part rhzomorph carrier mixing and stirring and get.
Wherein, described core rhzomorph prepares by the following method:
With the original strain of Rhodopseudomonas palustris, bacillus cereus and cereuisiae fermentum carry out successively respectively under the aseptic condition that slant culture, shaking table are cultivated, after the fermentor cultivation, the fermented liquid that obtains is obtained spore powder through concentrate drying, then with spore powder according to Rhodopseudomonas palustris: bacillus cereus: cereuisiae fermentum is that the weight ratio of 30-50:4-16:4-16 mixes, and obtains the core rhzomorph.
Preferably, with spore powder according to Rhodopseudomonas palustris: bacillus cereus: the weight ratio of cereuisiae fermentum 4:1:1 mixes, and obtains the core rhzomorph.
The application of cadmium pollution bioremediation agents of the present invention in farm crop produce,
In application, this cadmium pollution bioremediation agents is powdery or particulate state formulation, mixes thin loess or mix in the base manure and evenly spread fertilizer over the fields when ploughing soil, levels land, and average consumption is 30kg/hm
2
Beneficial effect of the present invention is:
1, microbe species collocation of the present invention is reasonable, and comprehensive function is stronger.Microorganism in the core rhzomorph of this invention can be rapidly field planting growth and form powerful bacterium colony in soil, directly Adsorption of Heavy Metal Ions cadmium Cd
+Solidified in a microorganism cells ancient piece of jade, round, flat and with a hole in its centre or the cell, passivation.Bacillus cereus in the combination and cereuisiae fermentum are to heavy metal ion cadmium Cd simultaneously
+, chromium Cr
+, plumbous Pb
+Stronger absorption passivation is also arranged, and stronger comprehensive function has reduced heavy metal ion cadmium Cd
+Movability (Dai Youzhi is permitted rosy clouds, and the natural science journal Vol.29No.3Sept.2007.79 of University Of Xiangtan～83 cereuisiae fermentum are to the absorption research of Cr in the water (VI); Pan Jianhua, Liu Ruixia, environmental science Vol.25, No.2Mar., the research of 2004.166～169 bacillus cereus Bacillus cereus Adsorption of Leads), reach and cut down crop to the absorption of heavy metal ion.
2, cadmium pollution bioremediation agents of the present invention technical office is in forward position leading in the world.Advantage function is to utilize Rhodopseudomonas palustris (being called for short the SRP bacterium), and directly S2-and the heavy metal ion reaction of absorption solidification, passivation and metabolism generation generate the sulfide precipitation of indissoluble and make heavy metal ion cadmium Cd
+The dual function that passivation is solidified: high price M+ (H) → low price M+H
+(M is heavy metal ion, and H is atomic hydrogen), SRP bacterium → SO
4 2-+ (H) → S
2-+ H
2O, M
2++ S
2-→ MS ↓.Because of the Rhodopseudomonas palustris surface with certain electronegativity, therefore to heavy metal ion cadmium Cd
+There are stronger adsorptivity and stronger biology to prohibit throwing out, heavy metal sulfide is solidified well, be difficult to move, realize cutting down crop to the absorption of heavy metal ion cadmium Cd+.
3, the advanced science of core rhzomorph preparation method of the present invention, simple and feasible, spore count content is high, and the survival time is long.Add enzyme being mass-energy and make spore rapidly field planting growth in soil, adding nutritive ingredient can provide energy for the spore amount reproduction.
Embodiment
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples just are used for explanation the present invention, rather than are used for limiting the scope of the invention.
The formation of embodiment 1 cadmium pollution bioremediation agents
15 parts of core rhzomorphs;
15 parts of nutritive ingredients;
4 parts of enzyme active materials;
66 parts in rhzomorph carrier.
The formation of described core rhzomorph
Rhodopseudomonas palustris 40.0 * 10
8Cfu/g;
Bacillus cereus 10.0 * 10
8Cfu/g;
Cereuisiae fermentum 10.0 * 10
8Cfu/g;
Effective bacterium total content 〉=60.0 * 10
8Cfu/g.
The core rhzomorph of above-mentioned formation is made by following steps:
Mix the preparation of spore powder:
1. slant culture: the original strain of Rhodopseudomonas palustris, bacillus cereus and cereuisiae fermentum is inoculated in respectively under aseptic condition on the slant medium, under 29 ± 1 ℃ of conditions, cultivates 40h;
2. shaking table is cultivated: the bacterial classification that 1. above-mentioned steps is cultivated is inoculated in respectively liquid nutrient medium under aseptic condition, be under 30 ℃ of conditions in pH6.8, temperature, and the 140r/min shaking table is cultivated 36h;
3. fermentor cultivation: the bacterial classification that 2. above-mentioned steps is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, under pH7.2, tank pressure 0.5kg, temperature are 30 ℃, ventilation 1:0.8 condition, cultivate 50h after, the bacterium number is greater than 1.0 * 10
10/ mL, 80% thalline change at present tank of gemma, obtain fermented liquid;
4. the fermented liquid that step is obtained in 3. is prepared into spore powder through concentrate drying, with spore powder according to Rhodopseudomonas palustris: bacillus cereus: cereuisiae fermentum is that the part by weight of 4:1:1 mixes, and prepares the core rhzomorph.
In the preparation of above-mentioned mixing spore powder, the prescription of the slant medium that step is selected in 1. is as follows: composition is NaH
2PO
45.5mg/L, oxysuccinic acid 1.5mg/L, sodium acetate 2mg/L, NaOH2mg/L, NH
4Cl1mg/L, MgCl
20.25mg/L, CaCl
20.05mg/L yeast extract paste 1mg/L, micro-1m1(trace element solution are iron protochloride, cobalt chloride, nickelous chloride, cupric chloride, Manganous chloride tetrahydrate, zinc chloride, Sodium orthomolybdate, Sodium selenite (Na2SeO3) pentahydrate composite solution), pH7.0.
The liquid culture based formulas of above-mentioned steps in 2. is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, K
2HPO
40.5g, MgSO
40.2g, water 1000mL.
The fermentor cultivation based formulas of above-mentioned steps in 3. is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, add water to 600kg.
Nutritive ingredient is nitrogen, phosphorus, and potassium, and zinc and iron, and according to N:P
2O
5: K
2O:ZnO:Fe
2O
3For mixing, the part by weight of 1.3:1.7:2:4:1 makes.
Described enzyme active material is protein powder and organized enzyme.Described enzyme active material is according to protein powder: organized enzyme is that the part by weight of 300:1～100:1 evenly is mixed; Preferably described enzyme active material is according to protein powder: organized enzyme is that the part by weight of 200:1 evenly is mixed.Organized enzyme is the organized enzyme that extracts from large pea green pod, and the method for extraction is: at first the first-class good blue or green pod of soybean is carried out the fragmentation of plant tissue cell.Adopt phosphoric acid buffer (pH value 6.0) to be mixed in the vessel according to solid-liquid ratio 1:5, the mixed solution that obtains filters (filter 23), removes solid impurity, obtains the crude extract of enzyme.Then the 58 ℃ of heating in water bath of crude extract to obtaining took out vessel after 10 minutes, left standstill 5 minutes, and the vessel bottom settlings goes out in the large pea green pod to contain solid impurity protein, gets supernatant liquor in another vessel, precipitates (pH value 5.8) with ammonium sulfate.Use at last phosphoric acid buffer (pH value 5.7) that the enzyme liquid that resolution of precipitate obtains is carried out separation and purification.
Described rhzomorph carrier is wilkinite: sepiolite powder evenly mixes in the ratio of 1:1.
With the ratio abundant mixing of above-mentioned materials according to 10 parts of core rhzomorphs, 10 parts of nutritive ingredients, 6 parts of enzyme active materials, 74 parts in rhzomorph carrier, prepare cadmium pollution bioremediation agents of the present invention.
The formation of embodiment 2 cadmium pollution bioremediation agents
20 parts of core rhzomorphs;
20 parts of nutritive ingredients;
5 parts of enzyme active materials;
55 parts in rhzomorph carrier.
The formation of described core rhzomorph
Rhodopseudomonas palustris 30.0 * 10
8Cfu/g;
Bacillus cereus 15.0 * 10
8Cfu/g;
Cereuisiae fermentum 15.0 * 10
8Cfu/g;
Effective bacterium total content 〉=60.0 * 10
8Cfu/g.
The preparation method:
1. slant culture: the original strain of Rhodopseudomonas palustris, bacillus cereus and cereuisiae fermentum is inoculated in respectively under aseptic condition on the slant medium, under 29 ± 1 ℃ of conditions, cultivates 48h;
2. shaking table is cultivated: the bacterial classification that 1. above-mentioned steps is cultivated is inoculated in respectively liquid nutrient medium under aseptic condition, be under 30 ℃ of conditions in pH7.0, temperature, and the 160r/min shaking table is cultivated 48h;
3. fermentor cultivation: the bacterial classification that 2. above-mentioned steps is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, under pH7.5, tank pressure 0.5kg, temperature are 30 ℃, ventilation 1:1.1 condition, cultivate 56h after, the bacterium number is greater than 1.0 * 10
10/ mL, 80% thalline change at present tank of gemma, obtain fermented liquid;
4. the fermented liquid that step is obtained in 3. is prepared into spore powder through concentrate drying, with spore powder according to Rhodopseudomonas palustris: bacillus cereus: cereuisiae fermentum is that the part by weight of 4:1:1 mixes, and prepares the core rhzomorph.
The used substratum of the used substratum of above-mentioned each step and embodiment 1 corresponding steps is identical, and the compound method of product is identical, and nutritive ingredient, enzyme active material, rhzomorph carrier are identical with embodiment 1, and just the ratio of preparation is different.
The formation of embodiment 3 cadmium pollution bioremediation agents
10 parts of core rhzomorphs;
10 parts of nutritive ingredients;
6 parts of enzyme active materials;
74 parts in rhzomorph carrier.
The formation of described core rhzomorph
Rhodopseudomonas palustris 50.0 * 10
8Cfu/g;
Bacillus cereus 5.0 * 10
8Cfu/g;
Cereuisiae fermentum 5.0 * 10
8Cfu/g;
Effective bacterium total content 〉=60.0 * 10
8Cfu/g.
The used substratum of the used substratum of each step and embodiment 1 corresponding steps was identical when above-mentioned enforcement preparation mixed spore powder, and the compound method of product is identical, and nutritive ingredient, enzyme active material, rhzomorph carrier are identical with embodiment 1, just the ratio of preparation difference.
The heavy metal cadmium bioremediation agents pot experiment of experimental example 1 embodiment of the invention 1
1 material and method
1.1 the employing pot experiment, the present invention is to different paddy soils heavy metal cadmium Cd in research
+The biological restoration effect.Test arranges 2 processing, and each processes triplicate, and random district group is arranged.
Process 1.: (amounting to the field consumption is 30kg/hm to use the heavy metal cadmium bioremediation agents 425mg/ basin of the embodiment of the invention 1
2);
Process and 2. contrast CK: the heavy metal cadmium bioremediation agents of not using the embodiment of the invention 1.
1.2 the white plastic basin alms bowl of 27cm * 30cm is used in test, soil sieves through air-dry 5 orders before the dress basin, every basin dress thick approximately 20cm of soil layer (kg) uses certain N,P,K fertilizers at every basin simultaneously, and it is consistent with field management that duration of test is respectively processed the cultivation management measure.
1.3 dress basin soil picks up from respectively Hengnan County, hengyang, hunan province city and presses down the wattenshlick field in husky mire village and the yellow mud field in Anfu village on the sunny side, the detected result before the experiment sees the following form 1.
Table 1 is for the front detected result of examination soil water experiment
1.4 Cadmium in Soil Cd
+Adopt CaCl
2Lixiviate one atomic absorption spectrophotometry (graphite furnace) is measured, and the cadmium in Brown Rice and the straw is the cadmium content after atomic absorption spectrophotometry (graphite furnace) mensuration is cleared up behind the employing nitric acid one perchloric acid Mixed Acids Digestion.
2 results and analysis
2.1 test-results shows, uses the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 to the cadmium Cd in the soil
+Reach the impact remarkable (seeing Table 2) of cadmium content in Brown Rice and the straw.Compare with the CK contrast, the heavy metal cadmium bioremediation agents of using inventive embodiments 1 can significantly reduce Cadmium in Soil Cd
+Content.Cadmium in Soil Cd behind the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 is used in the wattenshlick field
+Content has reduced by 0.096 than the CK contrast; Cadmium in Soil Cd behind the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 is used in the yellow mud field
+Content has reduced by 0.042 than the CK contrast.
Table 2 couple soil Cd
+Form affect the result
2.2 Cadmium in Soil Cd
+Be the Main Morphology that plant absorbing is utilized, the absorption that its content will obviously affect plant adds up.The result of table 3 shows, the heavy metal cadmium bioremediation agents of using the embodiment of the invention 1 can significantly reduce the cadmium content in Brown Rice and the straw, and makes the brown rice cadmium content reach national food hygienic standard (GB2715-2005).After the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 was used in the wattenshlick field, cadmium content had reduced by 49.1% and 50.6% than the CK contrast respectively in Brown Rice and the straw; After the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 was used in the yellow mud field, Brown Rice and straw cadmium content had reduced by 40.4% and 59.5% than the CK contrast respectively.
Table 3 is on the impact of cadmium content in Brown Rice and the straw
2.3 this shows, the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 is to heavy metal cadmium Cd
+Contaminated soil has obvious biological restoration effect, uses in right amount and can significantly reduce Cadmium in Soil Cd
+Content reduces the cumulative absorption of crop.
The effect of the cadmium pollution bioremediation agents of experimental example 2 embodiment 1 and aftereffect test
1 test basic condition and material
1.1 the Brown Rice during employing field plot trial, research the present invention were produced two seasons absorbs Cadmium Accumulation Cd
+Impact and after effect.Test site is selected in respectively that the Hengnan County, Hunan Province is pressed down husky mire village on the sunny side and the Anfu village carries out, and husky mire village soil is the moisture soil that river alluvium forms, and Anfu village soil is yellow mud field soil, and detected result sees Table 4 before the experiment.
Table 4 is for the front result of laboratory test of examination soil testing
1.2 test arranges 3 processing, does not establish repetition, establishes the protective belt all around, the wide ridge of 0.5m interval is established in the minizone.
1. contrast CK: the heavy metal cadmium bioremediation agents of not using the embodiment of the invention 1;
2. use the heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1
2;
3. use the heavy metal cadmium bioremediation agents 30kg/hm of the embodiment of the invention 1
2
1.3 the first season, early rice was used the heavy metal cadmium bioremediation agents of the embodiment of the invention 1, the second season, late rice was not used.Concrete application process is for before this cadmium pollution moisture soil is turned over fertilising, and the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 is mixed thin loess evenly to be spread fertilizer over the fields at soil surface, or spreads fertilizer over the fields with using base manure.The chemical fertilizer of all processing, the applications of pesticide and irrigation management are all consistent.Atomic absorption spectrophotometry (graphite furnace) was measured after cadmium content in Brown Rice and the straw adopted nitric acid one perchloric acid Mixed Acids Digestion.
2 results and analysis
2.1 to the first season early rice action effect obviously utilize the cadmium pollution bioremediation agents of the embodiment of the invention 1 that paddy soil is tested after, the test effect of early rice sees Table 2 to the first season, result's demonstration, the heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1 is used in the wattenshlick field
2And 30kg/hm
2Cadmium content reduces by 27.1% and 50.6% than the CK contrast respectively in the Brown Rice of processing; The heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1 is used in the yellow mud field
2And 30kg/hm
2Cadmium content reduces by 23.2% and 42.4% than the CK contrast respectively in the Brown Rice of processing.Wherein use the heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1
2And 30kg/hm
2Cadmium content all reaches national food hygienic standard (GB2715-2005) in the Brown Rice of processing.Therefore, the cadmium pollution bioremediation agents of the employing embodiment of the invention 1 can significantly reduce the cadmium content in the Brown Rice, illustrates the cadmium Cd in the paddy soil of Cadmium Pollution in Soils
+Has obvious repairing effect.
2.2 the aftereffect of late rice is obvious to the second season: after utilizing the cadmium pollution bioremediation agents of the embodiment of the invention 1 that paddy soil is tested, to the second season late rice planting experiment result such as table 5, the result shows, cadmium content still significantly is lower than contrast in the second season late rice brown rice.Wherein, the heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1 is used in the wattenshlick field
2And 30kg/hm
2Cadmium content reduces by 28.3% and 33.2% than the CK contrast respectively in the late rice brown rice in the treatment zone; The heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1 is used in the yellow mud field
2And 30kg/hm
2Cadmium content reduces by 28.2% and 33.9% than the CK contrast respectively in the late rice brown rice in the treatment zone.The cadmium pollution bioremediation agents that explanation is used the embodiment of the invention 1 at double rice cropping system has preferably after effect.
Effect and the after effect of table 5 cadmium pollution bioremediation agents
2.3 test-results shows, uses the heavy metal cadmium bioremediation agents 24kg/hm of the embodiment of the invention 1 in wattenshlick field and yellow mud field
2And 30kg/hm
2Cadmium content all has reduction than the CK contrast respectively in the late rice brown rice in the treatment zone, but consumption is 24kg/hm
2Processing 2. in the brown rice of its after effect cadmium content do not reach national food hygienic standard (GB2715-2005).Therefore suggestion is used the heavy metal cadmium bioremediation agents of the embodiment of the invention 1 with 30kg/hm
2Be advisable.
Although the above describes the present invention in detail with most preferred embodiment, those skilled in the art should know, and under the prerequisite that does not depart from inventive concept and spirit, any improvement and modification to the present invention makes still belong within the scope of protection of present invention.
Claims (10)
1. a cadmium pollution bioremediation agents is characterized in that, described cadmium pollution bioremediation agents is grouped into by following one-tenth by weight:
Core rhzomorph 10-20 part;
Nutritive ingredient 10-20 part;
Enzyme active material 2-6 part;
Rhzomorph carrier 54-78 part;
Wherein, described core rhzomorph is at least a among Rhodopseudomonas palustris (Rhodop seudanonas palustris) preserving number ACCC10649, preserving number ACCC00309, the preserving number ACCC00311, bacillus cereus (Bacillus cereus) preserving number ACCC02803, at least a combination among cereuisiae fermentum (Sacchaeomyces cerevisiae) preserving number ACCC21144, the preserving number ACCC21139.
2. cadmium pollution bioremediation agents according to claim 1 is characterized in that, described cadmium pollution bioremediation agents is grouped into by following one-tenth by weight:
15 parts of core rhzomorphs;
15 parts of nutritive ingredients;
4 parts of enzyme active materials;
66 parts in rhzomorph carrier.
3. cadmium pollution bioremediation agents according to claim 1 and 2 is characterized in that, the core rhzomorph in the described cadmium pollution bioremediation agents comprises Rhodopseudomonas palustris 30.0～50.0 * 10
8Cfu/g, bacillus cereus 4.0～16.0 * 10
8Cfu/g, cereuisiae fermentum 4.0～16.0 * 10
8Cfu/g, total living bacteria count 〉=60.0 * 10
8Cfu/g; Be preferably Rhodopseudomonas palustris 40.0 * 10
8Cfu/g, bacillus cereus 10.0 * 10
8Cfu/g, cereuisiae fermentum 10.0 * 10
8Cfu/g, total living bacteria count 〉=60.0 * 10
8Cfu/g.
4. cadmium pollution bioremediation agents according to claim 1 is characterized in that, described nutritive ingredient is nitrogen, phosphorus, potassium, and zinc and iron; Described nitrogen, phosphorus, potassium, and the part by weight of zinc and iron is N:P
2O
5: K
2O:ZnO:Fe
2O
3=1.3:1.7:2:4:1.
5. cadmium pollution bioremediation agents according to claim 1 is characterised in that described enzyme active material is protein powder and organized enzyme.Described enzyme active material is according to protein powder: organized enzyme is that the part by weight of 300:1～100:1 evenly is mixed; Preferably described enzyme active material is according to protein powder: organized enzyme is that the part by weight of 200:1 evenly is mixed; Described organized enzyme is the organized enzyme that extracts from large pea green pod.
6. cadmium pollution bioremediation agents according to claim 1 is characterized in that, described rhzomorph carrier is wilkinite: sepiolite powder evenly mixes in the ratio of 1:0.8～1.5; Described cadmium pollution bioremediation agents is pulvis or granule.
7. the preparation method of the described cadmium pollution bioremediation agents of claim 1-6 any one, it is characterized in that, it is with by weight 10-20 part core rhzomorph, 10-20 part nutritive ingredient, 2-6 part enzyme active material, 54-78 part rhzomorph carrier mixing and stirring and get.
8. preparation method according to claim 7 is characterized in that, described core rhzomorph prepares by the following method:
With the original strain of Rhodopseudomonas palustris, bacillus cereus and cereuisiae fermentum carry out successively respectively under the aseptic condition that slant culture, shaking table are cultivated, after the fermentor cultivation, the fermented liquid that obtains is obtained spore powder through concentrate drying, then with spore powder according to Rhodopseudomonas palustris: bacillus cereus: cereuisiae fermentum is that the weight ratio of 30-50:4-16:4-16 mixes, and obtains the core rhzomorph.
9. preparation method according to claim 8 is characterized in that, with spore powder according to Rhodopseudomonas palustris: bacillus cereus: the weight ratio of cereuisiae fermentum 4:1:1 mixes, and obtains the core rhzomorph.
10. the application of each described cadmium pollution bioremediation agents of claim 1-6 in farm crop produce.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310289456.XA CN103361072B (en) | 2013-07-10 | 2013-07-10 | Cadmium pollution bioremediation agent and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310289456.XA CN103361072B (en) | 2013-07-10 | 2013-07-10 | Cadmium pollution bioremediation agent and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103361072A true CN103361072A (en) | 2013-10-23 |
| CN103361072B CN103361072B (en) | 2016-03-16 |
Family
ID=49363353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310289456.XA Expired - Fee Related CN103361072B (en) | 2013-07-10 | 2013-07-10 | Cadmium pollution bioremediation agent and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103361072B (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555336A (en) * | 2013-10-31 | 2014-02-05 | 湖南泰谷生物科技股份有限公司 | Soil conditioner for reducing cadmium content in crop and preparation method and application thereof |
| CN103911162A (en) * | 2013-12-27 | 2014-07-09 | 新疆德蓝股份有限公司 | Magnetic filling material for soil remediation |
| CN104140824A (en) * | 2014-08-06 | 2014-11-12 | 湖南原生生物科技股份有限公司 | Biological organic fertilizer with high-cadmium-pollution-resistance bacterial strains and preparation method and application of biological organic fertilizer |
| CN104498042A (en) * | 2014-11-24 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Microorganism repairing agent and applications thereof in chromium polluted soil restoration |
| CN104560804A (en) * | 2014-12-25 | 2015-04-29 | 洪湖市添瑞生物制品厂 | Mixed microbial agent for soil remediation, and preparation method and application thereof |
| CN105016873A (en) * | 2015-07-15 | 2015-11-04 | 中国科学院地理科学与资源研究所 | Compound mixed biological bacterial fertilizer for restoring cadmium contaminated soil and preparation method and application thereof |
| CN105191715A (en) * | 2015-08-12 | 2015-12-30 | 中国科学院武汉植物园 | Method for reducing cadmium content of rice grains in cadmium-polluted rice field by using cadmium-resisting microorganisms |
| CN108192631A (en) * | 2017-12-26 | 2018-06-22 | 湖南恒凯环保科技投资有限公司 | A kind of charcoal base flora composite soil conditioner and its preparation and application |
| CN108913155A (en) * | 2018-08-17 | 2018-11-30 | 湖北茂盛生物有限公司 | A kind of microbial bacterial agent and preparation method thereof with soil remediation function |
| CN109554305A (en) * | 2018-08-27 | 2019-04-02 | 河北经贸大学 | A kind of screening technique, renovation agent and its application of probiotics that simulating Tiny ecosystem |
| CN109628324A (en) * | 2018-12-29 | 2019-04-16 | 天津天丰泽田生物科技有限公司 | A kind of bioremediation agents and preparation method of cadmium pollution |
| CN110252803A (en) * | 2019-07-23 | 2019-09-20 | 中国科学院成都生物研究所 | A kind of cadmium pollution soil composite passivant and its application |
| CN111925808A (en) * | 2020-03-17 | 2020-11-13 | 徐州工程学院 | Soil remediation agent based on high cadmium-resistant bacterial strain |
| CN114790432A (en) * | 2022-05-08 | 2022-07-26 | 桂林理工大学 | Bacillus cereus and method for preparing efficient lead-zinc wastewater flocculant by using same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497870A (en) * | 2009-03-02 | 2009-08-05 | 北京市京圃园生物工程有限公司 | Microbial complex bacterial agent and use thereof |
| CN102660479A (en) * | 2012-05-11 | 2012-09-12 | 娄底市裕德科技有限公司 | Compound microbial inoculant for composting fermentation, solid-state fermentation production method for compound microbial inoculant and application of compound microbial inoculant |
| CN102964178A (en) * | 2012-11-13 | 2013-03-13 | 湖南泰谷生物科技股份有限公司 | Composite microbial bactericide, and preparation method and application thereof |
-
2013
- 2013-07-10 CN CN201310289456.XA patent/CN103361072B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497870A (en) * | 2009-03-02 | 2009-08-05 | 北京市京圃园生物工程有限公司 | Microbial complex bacterial agent and use thereof |
| CN102660479A (en) * | 2012-05-11 | 2012-09-12 | 娄底市裕德科技有限公司 | Compound microbial inoculant for composting fermentation, solid-state fermentation production method for compound microbial inoculant and application of compound microbial inoculant |
| CN102964178A (en) * | 2012-11-13 | 2013-03-13 | 湖南泰谷生物科技股份有限公司 | Composite microbial bactericide, and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 贠妮: "《沼泽红假单胞菌（Rhodopseudomonas palustris）转化去除铅镉污染的研究》", 《中北大学硕士学位论文》, 31 December 2007 (2007-12-31) * |
| 陈志: "《蜡状芽孢杆菌与重金属铬相互作用机制研究》", 《福建林业大学硕士学位论文》, 31 December 2010 (2010-12-31) * |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555336B (en) * | 2013-10-31 | 2016-03-16 | 湖南泰谷生物科技股份有限公司 | Cut down soil conditioner of cadmium content and its preparation method and application in crop |
| CN103555336A (en) * | 2013-10-31 | 2014-02-05 | 湖南泰谷生物科技股份有限公司 | Soil conditioner for reducing cadmium content in crop and preparation method and application thereof |
| CN103911162A (en) * | 2013-12-27 | 2014-07-09 | 新疆德蓝股份有限公司 | Magnetic filling material for soil remediation |
| CN104140824B (en) * | 2014-08-06 | 2018-08-24 | 湖南原生生物科技股份有限公司 | The bio-organic fertilizer and its preparation method and application of the bacterial strain of cadmium pollution containing highly resistance |
| CN104140824A (en) * | 2014-08-06 | 2014-11-12 | 湖南原生生物科技股份有限公司 | Biological organic fertilizer with high-cadmium-pollution-resistance bacterial strains and preparation method and application of biological organic fertilizer |
| CN104498042A (en) * | 2014-11-24 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Microorganism repairing agent and applications thereof in chromium polluted soil restoration |
| CN104560804A (en) * | 2014-12-25 | 2015-04-29 | 洪湖市添瑞生物制品厂 | Mixed microbial agent for soil remediation, and preparation method and application thereof |
| CN105016873A (en) * | 2015-07-15 | 2015-11-04 | 中国科学院地理科学与资源研究所 | Compound mixed biological bacterial fertilizer for restoring cadmium contaminated soil and preparation method and application thereof |
| CN105191715A (en) * | 2015-08-12 | 2015-12-30 | 中国科学院武汉植物园 | Method for reducing cadmium content of rice grains in cadmium-polluted rice field by using cadmium-resisting microorganisms |
| CN108192631A (en) * | 2017-12-26 | 2018-06-22 | 湖南恒凯环保科技投资有限公司 | A kind of charcoal base flora composite soil conditioner and its preparation and application |
| CN108913155A (en) * | 2018-08-17 | 2018-11-30 | 湖北茂盛生物有限公司 | A kind of microbial bacterial agent and preparation method thereof with soil remediation function |
| CN109554305A (en) * | 2018-08-27 | 2019-04-02 | 河北经贸大学 | A kind of screening technique, renovation agent and its application of probiotics that simulating Tiny ecosystem |
| CN109554305B (en) * | 2018-08-27 | 2022-06-07 | 河北经贸大学 | Screening method of probiotics simulating micro-ecology, repairing agent and application of repairing agent |
| CN109628324A (en) * | 2018-12-29 | 2019-04-16 | 天津天丰泽田生物科技有限公司 | A kind of bioremediation agents and preparation method of cadmium pollution |
| CN110252803A (en) * | 2019-07-23 | 2019-09-20 | 中国科学院成都生物研究所 | A kind of cadmium pollution soil composite passivant and its application |
| CN110252803B (en) * | 2019-07-23 | 2021-04-30 | 中国科学院成都生物研究所 | Cadmium-contaminated soil composite passivator and application thereof |
| CN111925808A (en) * | 2020-03-17 | 2020-11-13 | 徐州工程学院 | Soil remediation agent based on high cadmium-resistant bacterial strain |
| CN114790432A (en) * | 2022-05-08 | 2022-07-26 | 桂林理工大学 | Bacillus cereus and method for preparing efficient lead-zinc wastewater flocculant by using same |
| CN114790432B (en) * | 2022-05-08 | 2023-06-23 | 桂林理工大学 | Bacillus cereus and method for preparing efficient lead-zinc wastewater flocculant by using same |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103361072B (en) | 2016-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103361072B (en) | Cadmium pollution bioremediation agent and its preparation method and application | |
| CN108624539B (en) | Straw and cow dung ultrahigh-temperature decomposition agent and preparation and application thereof | |
| CN105623670B (en) | A kind of heavy metal deactivator and its application | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN103819237B (en) | Tobacco special microorganism is fertile and preparation method thereof | |
| CN103626610B (en) | A kind of Biologic organic bamboo charcoal fertilizer and production method thereof | |
| CN103255078B (en) | Soil heavy metal biodegradation agent and preparation method thereof | |
| CN102172183B (en) | Seedling culturing substrate block and preparation method thereof | |
| CN106701095B (en) | Soil conditioner and application thereof in desert control | |
| CN103589435B (en) | For administering native regulatory agent of farmland soil heavy metals pollution and preparation method thereof | |
| CN106495951A (en) | A kind of high concentration microorganism microbial inoculum containing polyglutamic acid and preparation method and application | |
| CN103787776A (en) | Biological water flushing fertilizer and preparation method thereof | |
| CN103238440A (en) | Method for reducing cadmium pollution risk for rape | |
| CN105420116A (en) | Efficient phosphorus-dissolving growth promoting bacterium, bio-organic fertilizer prepared from efficient phosphorus-dissolving growth promoting bacterium and application | |
| CN109628437A (en) | Reduce the composite passivant and the preparation method and application thereof of soil cadmium content | |
| CN105798059A (en) | Method for treating heavy metal in soil | |
| CN103555336B (en) | Cut down soil conditioner of cadmium content and its preparation method and application in crop | |
| CN103224418A (en) | Blue alga compost and its making method | |
| CN105948972A (en) | Amino acid fertilizer and preparation method and application thereof | |
| CN112592866A (en) | Carbon-based microbial agent for soil remediation and preparation method and application thereof | |
| CN110117204A (en) | Farmland soil heavy metals degradation agent | |
| CN102515954A (en) | Special biological organic fertilizer for verticillium wilt and production method for same | |
| CN105820008A (en) | Soil restoration conditioner for heavy metal pollution and preparation method thereof | |
| CN107502370A (en) | A kind of preparation method of composite soil modifier | |
| CN103834409A (en) | Passivating agent for mercury-polluted soil remediation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Cadmium pollution bioremediation agent as well as preparation method and application thereof Effective date of registration: 20170621 Granted publication date: 20160316 Pledgee: Changsha rural commercial bank Limited by Share Ltd. Lugu branch Pledgor: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. Registration number: 2017430000033 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20180629 Granted publication date: 20160316 Pledgee: Changsha rural commercial bank Limited by Share Ltd. Lugu branch Pledgor: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. Registration number: 2017430000033 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 410000 Hunan Changsha national hi tech Industrial Development Zone, 27 Yuan Lu Yu Yuan Pioneer Building 1506, 1507 Patentee after: TAIGU BIOTECHNOLOGY GROUP Co.,Ltd. Address before: 410205 A 14 14, Lulong Road, Changsha, Hunan. Patentee before: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20131023 Assignee: HUNAN TAIGU ECOLOGICAL ENGINEERING Co.,Ltd. Assignor: TAIGU BIOTECHNOLOGY GROUP Co.,Ltd. Contract record no.: 2018430000013 Denomination of invention: Cadmium pollution bioremediation agent as well as preparation method and application thereof Granted publication date: 20160316 License type: Exclusive License Record date: 20180713 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Cadmium pollution bioremediation agent as well as preparation method and application thereof Effective date of registration: 20180813 Granted publication date: 20160316 Pledgee: Changsha bank Limited by Share Ltd. Xiangjiang New District sub branch Pledgor: TAIGU BIOTECHNOLOGY GROUP Co.,Ltd. Registration number: 2018430000056 |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160316 |