CN111925808A - Soil remediation agent based on high cadmium-resistant bacterial strain - Google Patents
Soil remediation agent based on high cadmium-resistant bacterial strain Download PDFInfo
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- CN111925808A CN111925808A CN202010185278.6A CN202010185278A CN111925808A CN 111925808 A CN111925808 A CN 111925808A CN 202010185278 A CN202010185278 A CN 202010185278A CN 111925808 A CN111925808 A CN 111925808A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention relates to the technical field of soil remediation treatment, and particularly discloses a soil remediation agent based on a high cadmium-resistant strain, which is prepared from the following raw materials in parts by weight: the microbial agent is prepared from a high cadmium-tolerant strain QN-3 separated from polluted soil, and the composite powder comprises the following components in parts by weight: 50-80 parts of modified concave-convex soil, 12-20 parts of diatomite, 20-26 parts of crop straws, 15-22 parts of poultry and livestock manure and 5-8 parts of sugar residues, wherein the high cadmium-resistant strain QN-3 is preserved in Guangdong province microbial culture collection center (GDMCC) in 2018, 05 and 28 months, and the strain deposition number is GDMCC No: 60378. the invention overcomes the defects of the prior art, can obviously improve the content of organic matters in the polluted soil by utilizing the matching of the microorganisms and the composite powder, adjusts the flora structure and the alkalinity and acidity of the soil, improves the air permeability of the soil, improves the physical and chemical properties of the soil, and can effectively degrade the content of organic pollutants and heavy metals in the soil.
Description
Technical Field
The invention relates to the technical field of soil remediation treatment, and particularly belongs to a soil remediation agent based on a high cadmium-resistant strain.
Background
The soil is a loose surface layer which has fertility and can grow plants on the surface of the land, the thickness of the soil is about 2m generally, and the soil not only provides mechanical support capability for the growth of the plants, but also provides fertility factors such as water, fertilizer, gas, heat and the like required by the growth and development of the plants. In recent years, as the population is rapidly increased and the industry is rapidly developed, solid waste is continuously piled and dumped to the surface of soil, harmful waste water is continuously permeated into the soil, and waste gas discharged by automobiles, harmful gas and floating dust in the atmosphere are continuously fallen in the soil along with rainwater. The improvement of the level of agricultural chemistry causes a large amount of chemical fertilizers and pesticides to scatter into the environment, so that the probability that the soil is polluted by non-point sources is increased, the degree of the pollution is increased, and the polluted area is enlarged continuously under the influence of water and soil loss, wind erosion and the like. When the harmful substances in the soil are excessive and exceed the self-cleaning capacity of the soil, the composition, the structure and the function of the soil are changed, the microbial activity is inhibited, the harmful substances or decomposition products thereof are gradually accumulated in the soil and absorbed by the human body through a biological circulation way, and the degree harmful to the health of the human body is reached, namely the soil pollution.
The remediation of heavy metal contaminated soil is mainly based on 2 strategies: firstly, removing heavy metals from soil to achieve the aim of cleaning the soil; and secondly, the heavy metal is immobilized in the soil to limit the release of the heavy metal, so that the risk of the heavy metal is reduced. In recent years, research on remediation technologies for heavy metal contaminated soil has been greatly developed, and the remediation technologies are mainly classified into physical/chemical remediation, biological remediation and agricultural ecological remediation according to process principles.
The microbial remediation utilizes indigenous or exogenous microbe groups to promote or strengthen the degradation function of microbes under proper environmental conditions, thereby achieving the biological remediation technology of reducing the activity of toxic pollutants or degrading the toxic pollutants into nontoxic substances. Therefore, the development of a microbial soil remediation agent with low cost, simplicity, convenience, high efficiency and environmental friendliness has important significance and urgency in China.
Disclosure of Invention
The invention aims to provide a soil remediation agent based on a high cadmium-resistant strain, overcomes the defects of the prior art, can obviously improve the content of organic matters in polluted soil by utilizing the cooperation of microorganisms and composite powder, regulates the flora structure and the alkalinity and acidity of the soil, improves the air permeability of the soil, improves the physicochemical property of the soil, and can effectively degrade the content of the organic pollutants and heavy metals in the soil.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a soil remediation agent based on a high cadmium-resistant strain is prepared from the following raw materials in parts by weight: the microbial agent is prepared from a high cadmium-tolerant strain QN-3 separated from polluted soil, and the composite powder comprises the following components in parts by weight: 50-80 parts of modified concave-convex soil, 12-20 parts of diatomite, 20-26 parts of crop straws, 15-22 parts of poultry and livestock manure and 5-8 parts of sugar residues.
Further, the high cadmium-tolerant strain QN-3 is deposited in Guangdong province microbial culture collection center (GDMCC) in 2018, 05 and 28 months, and the strain deposit number is GDMCC No: 60378.
further, the preparation method of the modified attapulgite comprises the following steps: weighing a certain amount of concavo-convex holding soil, adding a polyvinylpyrrolidone solution with the mass fraction of 0.1-0.2% and a sodium borohydride solution with the mass fraction of 0.2-0.3%, stirring uniformly, keeping the temperature for 8-12 minutes at 80-120 ℃, taking out, cooling, adding white vinegar, mixing uniformly, finally putting into a freeze dryer to dry into a solid, and crushing the solid into powder to obtain the modified concavo-convex holding soil.
Further, the preparation method of the soil remediation agent comprises the following steps:
(1) preparation of the microbial agent: under the aseptic condition, in a sterilized container, a high cadmium-resistant strain QN-3 is inoculated into a liquid culture medium according to 4.5 percent of the mass of the liquid culture medium, shaking culture is carried out for 3 days at the temperature of 22-25 ℃ to obtain a seed bacterial liquid, then the seed bacterial liquid is inoculated into the liquid culture medium according to the inoculation amount of 9 percent, shaking culture is carried out for 3-5 days at the temperature of 28-30 ℃ to obtain a zymocyte liquid, and the zymocyte liquid is directly frozen and dried to obtain the microbial agent.
(2) Preparing composite powder: weighing the modified attapulgite, the diatomite, the crop straws, the poultry and livestock excrements and the sugar residues in sequence according to the weight parts, respectively putting the raw materials into a crusher for crushing, then putting the raw materials into a stirring kettle for uniformly stirring, placing the mixture for 3 to 5 days, drying, crushing and sieving to obtain the composite powder.
Further, the poultry and livestock manure is any one or more of pig manure, cow manure, sheep manure, chicken manure or duck manure, and the crop straw is any one or more of wheat straw, cotton straw, corn straw or soybean straw.
Further, the using method of the soil remediation agent comprises the following steps: spreading a layer of composite powder on the surface of the soil seriously polluted by cadmium to form a composite powder layer with the thickness of 5-8mm, then uniformly spraying a microbial agent on the composite powder layer, turning, uniformly mixing and replenishing water, standing for a period of time, periodically spraying water to keep the water content of the soil and periodically stirring the soil.
Further, the weight of the microbial agent sprayed on 1kg of the composite powder is 100-200 g.
Compared with the prior art, the invention has the following implementation effects:
according to the soil remediation agent based on the high cadmium-resistant bacterial strain, the content of organic matters in the polluted soil can be obviously increased by using the cooperation of the microorganism and the composite powder, the microbial flora structure and the soil acidity and alkalinity are adjusted, the soil permeability is improved, the physical and chemical properties of the soil are improved, and the contents of organic pollutants and heavy metals in the soil can be effectively degraded; meanwhile, the fertilizer has the advantages of being beneficial to nutrient maintenance, prolonging the fertilizer efficiency, promoting the absorption of crops to nutrients, increasing the crop yield, improving the soil fertility and having obvious effects of increasing the yield and the income of the crops; the microorganism adopts a high cadmium-resistant strain separated from the heavy metal contaminated soil, and all components of the composite powder are common material components, so that the cost is reduced, and the composite powder has great social and economic values.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to these examples, and any modification is within the scope of the present invention without departing from the spirit of the present invention.
A soil remediation agent based on a high cadmium-resistant strain:
the traditional Chinese medicine is prepared from the following raw materials in parts by weight: the microbial agent is prepared from a high cadmium-tolerant strain QN-3 separated from polluted soil, and the composite powder comprises the following components in parts by weight: 50-80 parts of modified concave-convex soil, 12-20 parts of diatomite, 20-26 parts of crop straws, 15-22 parts of poultry and livestock manure and 5-8 parts of sugar residues.
The high cadmium-resistant strain QN-3 is preserved in Guangdong province microorganism culture collection center (GDMCC) in 2018, 05 and 28 days, and the strain preservation number is GDMCC No: 60378, proposed taxonomy name: cystobasidium oligophagu, deposit unit address: experimental building 5 of 100 # large college of junior furious district of Guangzhou city, Guangdong province.
The preparation method of the soil remediation agent comprises the following steps:
(1) preparation of the microbial agent: under the aseptic condition, in a sterilized container, a high cadmium-resistant strain QN-3 is inoculated into a liquid culture medium according to 4.5 percent of the mass of the liquid culture medium, shaking culture is carried out for 3 days at the temperature of 22-25 ℃ to obtain a seed bacterial liquid, then the seed bacterial liquid is inoculated into the liquid culture medium according to the inoculation amount of 9 percent, shaking culture is carried out for 3-5 days at the temperature of 28-30 ℃ to obtain a zymocyte liquid, and the zymocyte liquid is directly frozen and dried to obtain the microbial agent.
(2) Preparing composite powder: weighing the modified attapulgite, the diatomite, the crop straws, the poultry and livestock excrements and the sugar residues in sequence according to the weight parts, respectively putting the raw materials into a crusher for crushing, then putting the raw materials into a stirring kettle for uniformly stirring, placing the mixture for 3 to 5 days, drying, crushing and sieving to obtain the composite powder.
The preparation method of the modified attapulgite clay comprises the following steps: weighing a certain amount of concavo-convex holding soil, adding a polyvinylpyrrolidone solution with the mass fraction of 0.1-0.2% and a sodium borohydride solution with the mass fraction of 0.2-0.3%, stirring uniformly, keeping the temperature for 8-12 minutes at 80-120 ℃, taking out, cooling, adding white vinegar, mixing uniformly, finally putting into a freeze dryer to dry into a solid, and crushing the solid into powder to obtain the modified concavo-convex holding soil.
The poultry and livestock manure is one or more of pig manure, cow manure, sheep manure, chicken manure or duck manure, and the crop straw is one or more of wheat straw, cotton straw, corn straw or soybean straw.
The using method of the soil remediation agent comprises the following steps: spreading a layer of composite powder on the surface of the soil seriously polluted by cadmium to form a composite powder layer with the thickness of 5-8mm, then uniformly spraying a microbial agent on the composite powder layer, turning, uniformly mixing and replenishing water, standing for a period of time, periodically spraying water to keep the water content of the soil and periodically stirring the soil; the weight of the microbial agent sprayed on 1kg of the composite powder is 100-200 g.
Example 1
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 1 comprises the following components in parts by weight: 50 parts of modified concave-convex soil, 12 parts of diatomite, 20 parts of crop straws, 15 parts of poultry and livestock manure and 5 parts of sugar residues; when in use, the thickness of the composite powder layer is 5mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 100 g.
Example 2
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 2 comprises the following components in parts by weight: 65 parts of modified concave-convex soil, 16 parts of diatomite, 23 parts of crop straws, 16.5 parts of poultry and livestock manure and 6.5 parts of sugar residues; when in use, the thickness of the composite powder layer is 5mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 100 g.
Example 3
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 2 comprises the following components in parts by weight: 80 parts of modified concave-convex soil, 20 parts of diatomite, 26 parts of crop straws, 22 parts of poultry and livestock manure and 8 parts of sugar residues; when in use, the thickness of the composite powder layer is 5mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 100 g.
Example 4
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 2 comprises the following components in parts by weight: 80 parts of modified concave-convex soil, 20 parts of diatomite, 26 parts of crop straws, 22 parts of poultry and livestock manure and 8 parts of sugar residues; when in use, the thickness of the composite powder layer is 8mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 100 g.
Example 5
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 2 comprises the following components in parts by weight: 80 parts of modified concave-convex soil, 20 parts of diatomite, 26 parts of crop straws, 22 parts of poultry and livestock manure and 8 parts of sugar residues; when in use, the thickness of the composite powder layer is 5mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 200 g.
Example 6
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 2 comprises the following components in parts by weight: 80 parts of modified concave-convex soil, 20 parts of diatomite, 26 parts of crop straws, 22 parts of poultry and livestock manure and 8 parts of sugar residues; when in use, the thickness of the composite powder layer is 8mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 200 g.
Example 7
In this embodiment, the soil remediation agent is prepared according to the method of the present invention, and the compound powder of embodiment 2 comprises the following components in parts by weight: 80 parts of modified concave-convex soil, 20 parts of diatomite, 26 parts of crop straws, 22 parts of poultry and livestock manure and 8 parts of sugar residues; when in use, the thickness of the composite powder layer is 5mm, and the weight of the microbial agent sprayed on 1kg of the composite powder is 0 g.
Example of effects:
(1) experimental samples: the soil remediation agent prepared in examples 1 to 7 of the present invention.
(2) The experimental method comprises the following steps: heavy metal contaminated soil: the soil with heavy cadmium pollution of 7 mu is selected from Quanwantoucun of Xuzhou city, Jiangsu province.
(a) Determining the cadmium content of various forms of heavy metal contaminated soil according to detection methods in a standard GB/T23739-2009 atomic absorption method for determining effective lead and cadmium in soil quality and technical specification for monitoring soil environment;
(b) the method comprises the steps of averagely dividing 7 mu of heavy metal contaminated soil into seven groups, respectively applying seven groups of experimental samples to the heavy metal contaminated soil according to a using method, ploughing and uniformly mixing to obtain mixed soil, respectively adding water into the mixed soil to enable the water content of the mixed soil to exceed 50%, covering a plastic film on the mixed soil, maintaining for 10 days to enable a repairing agent in the mixed soil to fully react with cadmium, fully stabilizing, respectively measuring the content of various forms of cadmium in the 7 groups of mixed soil, and measuring results are shown in table 1.
TABLE 1 statistics of cadmium morphological changes (mg/kg) of soil before and after remediation
(3) The experimental results are as follows: as can be seen from Table 1, the content of cadmium in an effective state in the heavy metal contaminated soil treated by the soil remediation agents prepared in the embodiments 1 to 5 meets GB 15618-.
The foregoing is merely exemplary and illustrative of the present inventive concept and various modifications, additions and substitutions of similar embodiments may be made to the specific embodiments described by those skilled in the art without departing from the inventive concept or exceeding the scope of the claims as defined in the accompanying claims.
Claims (7)
1. A soil remediation agent based on a high cadmium-resistant strain is characterized in that: the traditional Chinese medicine is prepared from the following raw materials in parts by weight: the microbial agent is prepared from a high cadmium-tolerant strain QN-3 separated from polluted soil, and the composite powder comprises the following components in parts by weight: 50-80 parts of modified concave-convex soil, 12-20 parts of diatomite, 20-26 parts of crop straws, 15-22 parts of poultry and livestock manure and 5-8 parts of sugar residues.
2. The soil remediation agent based on a highly cadmium tolerant strain of claim 1, wherein: the high cadmium-resistant strain QN-3 is preserved in Guangdong province microorganism culture collection center (GDMCC) in 2018, 05 and 28 days, and the strain preservation number is GDMCC No: 60378.
3. the soil remediation agent based on a highly cadmium tolerant strain of claim 1, wherein: the preparation method of the modified concave-convex bar soil comprises the following steps: weighing a certain amount of concavo-convex holding soil, adding a polyvinylpyrrolidone solution with the mass fraction of 0.1-0.2% and a sodium borohydride solution with the mass fraction of 0.2-0.3%, stirring uniformly, keeping the temperature for 8-12 minutes at 80-120 ℃, taking out, cooling, adding white vinegar, mixing uniformly, finally putting into a freeze dryer to dry into a solid, and crushing the solid into powder to obtain the modified concavo-convex holding soil.
4. The soil remediation agent based on a highly cadmium tolerant strain of claim 1, wherein: the preparation method of the soil remediation agent comprises the following steps:
(1) preparation of the microbial agent: under the aseptic condition, in a sterilized container, a high cadmium-resistant strain QN-3 is inoculated into a liquid culture medium according to 4.5 percent of the mass of the liquid culture medium, shaking culture is carried out for 3 days at the temperature of 22-25 ℃ to obtain a seed bacterial liquid, then the seed bacterial liquid is inoculated into the liquid culture medium according to the inoculation amount of 9 percent, shaking culture is carried out for 3-5 days at the temperature of 28-30 ℃ to obtain a zymocyte liquid, and the zymocyte liquid is directly frozen and dried to obtain the microbial agent.
(2) Preparing composite powder: weighing the modified attapulgite, the diatomite, the crop straws, the poultry and livestock excrements and the sugar residues in sequence according to the weight parts, respectively putting the raw materials into a crusher for crushing, then putting the raw materials into a stirring kettle for uniformly stirring, placing the mixture for 3 to 5 days, drying, crushing and sieving to obtain the composite powder.
5. The soil remediation agent based on a highly cadmium-tolerant strain of bacteria of claim 4, wherein: the poultry and livestock manure is one or more of pig manure, cow manure, sheep manure, chicken manure or duck manure, and the crop straw is one or more of wheat straw, cotton straw, corn straw or soybean straw.
6. The soil remediation agent based on a highly cadmium tolerant strain of claim 1, wherein: the using method of the soil remediation agent comprises the following steps: spreading a layer of composite powder on the surface of the soil seriously polluted by cadmium to form a composite powder layer with the thickness of 5-8mm, then uniformly spraying a microbial agent on the composite powder layer, turning, uniformly mixing and replenishing water, standing for a period of time, periodically spraying water to keep the water content of the soil and periodically stirring the soil.
7. The soil remediation agent based on a highly cadmium tolerant strain of claim 6, wherein: the weight of the microbial agent sprayed on 1kg of the composite powder is 100-200 g.
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| CN114196414A (en) * | 2021-12-25 | 2022-03-18 | 云南农业大学 | Environment-friendly soil heavy metal pollution repairing agent and repairing method |
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