CN105152700B - A kind of organic waste deodorizer based on wormcast Yu complex microorganism - Google Patents

A kind of organic waste deodorizer based on wormcast Yu complex microorganism Download PDF

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Publication number
CN105152700B
CN105152700B CN201510484089.8A CN201510484089A CN105152700B CN 105152700 B CN105152700 B CN 105152700B CN 201510484089 A CN201510484089 A CN 201510484089A CN 105152700 B CN105152700 B CN 105152700B
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wormcast
organic waste
parts
complex microorganism
bacteria
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CN105152700A (en
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倪宏波
王欣
闻晓波
翟军军
周玉龙
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Heilongjiang Bayi Agricultural University
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Heilongjiang Bayi Agricultural University
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Priority to CN201610640559.XA priority Critical patent/CN106220259B/en
Priority to CN201610640581.4A priority patent/CN106007828B/en
Priority to CN201510484089.8A priority patent/CN105152700B/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Abstract

The invention discloses a kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 80 120 parts, bacillus subtilis 12 parts, nitrite bacteria 12 parts, sulfur bacteria 12 parts, thiobacillus denitrificans 12 parts.In the present invention, beneficial microbe is with wormcast as growth promoter, the harmful substance having foul smell based on wormcast and the organic waste deodorizer decomposable asymmetric choice net ammonia of complex microorganism, amine salt, sulfide etc. obtained, based on mutual synergism of other materials such as microbial inoculums in the wormcast organic waste deodorizer with complex microorganism, do not only reach the purpose of decrement deodorization, organic waste can also be turned waste into wealth, produce biological organic fertilizer.The present invention is used for organic waste deodorization, decrement, and compared to existing technology, investment of production is little, and production cost is low, pure environmental protection, process effect height, wide adaptability, easy to use.

Description

A kind of organic waste deodorizer based on wormcast Yu complex microorganism
Technical field
The present invention relates to field of garbage disposal, a kind of organic waste deodorizer based on wormcast Yu complex microorganism.
Background technology
Rubbish is the garbage produced in people's daily life and production process, owing to output is big and complicated component is various, is subtracting Amount, deodorization process problem on brought the biggest difficulty.Process at present the technology of house refuse mainly have sanitary landfills, Burn and compost etc., wherein composting technology be that organic waste is innoxious, minimizing and the key technology of resource, and microorganism is The main body of compost fermentation.
Additionally, utilize microorganism to carry out deodorization environmental protection, it is to be decomposed by biological oxidation to eliminate source of odor, Deodorization is in extensive range, and suppresses anaerobe or the quantity of other harmful bacteria by biological inoculum balanced action so that it is cannot produce Raw stink.By microorganism and useful mushroom etc. decompose Organic substance, stop Organic substance produce ammonia and hydrogen sulfide (stink) with And the generation of harmful bacteria and breeding.
Microbial deodorant compares with traditional chemical product.The product that every kind of being both for property of chemical products is strong, when meeting complexity During other chemical matrix, will lose efficacy;After using chemical products, always having chemical residue in rubbish, it may bring pair Effect or new pollution;Use chemical products can cover stink, but can not change the generation of stink or stop it to distribute.Microorganism Deodorization technology is to utilize natural decomposition and the positive biochemical action in catabolic process, will not produce the problems referred to above.Microbial deodorant Agent is compared with traditional biological cleanser.Microbial deodorant technology can greatly dispel stink, makes the rapid metabolism of organic substance, Reducing bulk of solid matter, fast purification is contaminated material.
Microbial deodorization method has the superiority that traditional method is incomparable, such as treatment effeciency height, non-secondary pollution, required Equipment is simple, easily operation, low cost, management are easy to maintenance, and its development potentiality and application prospect are the most widely.
Summary of the invention
It is an object of the invention to provide a kind of organic waste deodorizer based on wormcast Yu complex microorganism, to solve the above-mentioned back of the body The problem proposed in scape technology.
For achieving the above object, the present invention provides following technical scheme:
A kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 80-120 Part, bacillus subtilis 1-2 part, nitrite bacteria 1-2 part, sulfur bacteria 1-2 part, thiobacillus denitrificans 1-2 part;It is prepared Method, concretely comprises the following steps:
(1) respectively bacillus subtilis, nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 120-180g/L, is placed in and there is stirring and temperature control merit In the fermentation tank of energy;
(3) bacillus subtilis good for activation culture in step (1), nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are connect Plant in fermentation medium prepared by step (2), amplification culture 24-72 hour under the rotating speed of 180-220rpm, cultivate temperature Degree is for 28-38 DEG C;
(4) colloid obtained is organic waste deodorizer based on wormcast Yu complex microorganism.
As the further scheme of the present invention: described organic waste deodorizer based on wormcast Yu complex microorganism, according to weight The raw material of part is: wormcast 90-110 part, bacillus subtilis 1.4-1.6 part, nitrite bacteria 1.4-1.6 part, sulfur bacteria 1.4-1.6 part, thiobacillus denitrificans 1.4-1.6 part.
As the further scheme of the present invention: described organic waste deodorizer based on wormcast Yu complex microorganism, according to weight Part raw material be: wormcast 100 parts, bacillus subtilis 1.5 parts, nitrite bacteria 1.5 parts, sulfur bacteria 1.5 parts, de- Nitrogen Thiobacillus 1.5 parts.
As the further scheme of the present invention: wormcast is prepared as the fermentation that mass concentration is 145-155g/L by step (2) Culture medium.
As the further scheme of the present invention: amplification culture 36-48 hour, cultivation under the rotating speed of 200rpm in step (3) Temperature is 32-35 DEG C.
Compared with prior art, the invention has the beneficial effects as follows:
In the present invention, beneficial microbe is with wormcast as growth promoter, the organic rubbish based on wormcast Yu complex microorganism obtained The harmful substance of the tool foul smell such as rubbish deodorizer decomposable asymmetric choice net ammonia, amine salt, sulfide, based on wormcast and complex microorganism organic In garbage deodorant, the mutual synergism of other materials such as microbial inoculum, has not only reached the purpose of decrement deodorization, it is also possible to by organic rubbish Rubbish is turned waste into wealth, and produces biological organic fertilizer.The present invention is used for organic waste deodorization, decrement, compared to existing technology, investment of production Little, production cost is low, pure environmental protection, process effect height, wide adaptability, easy to use.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the technical scheme of this patent is described in more detail.
Embodiment 1
A kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 80 parts, Bacillus subtilis 2 parts, nitrite bacteria 2 parts, sulfur bacteria 2 parts, thiobacillus denitrificans 2 parts.Its preparation method, specifically walks Suddenly it is:
(1) respectively bacillus subtilis, nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 180g/L, is placed in and there is stirring and function of temperature control In fermentation tank;
(3) bacillus subtilis good for activation culture in step (1), nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are connect Planting in fermentation medium prepared by step (2), amplification culture 72 hours under the rotating speed of 180rpm, cultivation temperature is 28 DEG C;
(4) colloid obtained is organic waste deodorizer based on wormcast Yu complex microorganism.
Embodiment 2
A kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 100 parts, Bacillus subtilis 1.5 parts, nitrite bacteria 1.5 parts, sulfur bacteria 1.5 parts, thiobacillus denitrificans 1.5 parts.Its preparation method, Concretely comprise the following steps:
(1) respectively bacillus subtilis, nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 150g/L, is placed in and there is stirring and function of temperature control In fermentation tank;
(3) bacillus subtilis good for activation culture in step (1), nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are connect Planting in fermentation medium prepared by step (2), amplification culture 48 hours under the rotating speed of 200rpm, cultivation temperature is 32 DEG C;
(4) colloid obtained is organic waste deodorizer based on wormcast Yu complex microorganism.
Embodiment 3
A kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 120 parts, Bacillus subtilis 1 part, nitrite bacteria 1 part, sulfur bacteria 1 part, thiobacillus denitrificans 1 part.Its preparation method, specifically walks Suddenly it is:
(1) respectively bacillus subtilis, nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 120g/L, is placed in and there is stirring and function of temperature control In fermentation tank;
(3) bacillus subtilis good for activation culture in step (1), nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are connect Planting in fermentation medium prepared by step (2), amplification culture 24 hours under the rotating speed of 220rpm, cultivation temperature is 38 DEG C;
(4) colloid obtained is organic waste deodorizer based on wormcast Yu complex microorganism.
Embodiment 4
A kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 100 parts, Bacillus subtilis 1 part, nitrite bacteria 1 part, sulfur bacteria 2 parts, thiobacillus denitrificans 2 parts.Its preparation method, with implementing Example 2.
Embodiment 5
A kind of organic waste deodorizer based on wormcast Yu complex microorganism, according to the raw material of weight portion be: wormcast 100 parts, Bacillus subtilis 2 parts, nitrite bacteria 2 parts, sulfur bacteria 1 part, thiobacillus denitrificans 1 part.Its preparation method, with implementing Example 2.
Comparative example 1
A kind of organic waste deodorizer, according to the raw material of weight portion be: fermentation medium 100 parts, bacillus subtilis 1.5 parts, Nitrite bacteria 1.5 parts, sulfur bacteria 1.5 parts, thiobacillus denitrificans 1.5 parts.Its preparation method, concretely comprises the following steps:
(1) respectively bacillus subtilis, nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are carried out activation culture;
(2) configuration fermentation medium, the formula of described fermentation medium is Semen Maydis powder 12g/L, glucose 5g/L, soybean cake powder 20g/L, fish flour 5g/L, calcium carbonate 7g/L, sulphuric acid money 1.Og/L, dipotassium hydrogen phosphate 0.3g/L, magnesium sulfate 0.2g/L and sulfur Acid manganese 0.2g/L, is placed in the fermentation tank with stirring and function of temperature control;
(3) bacillus subtilis good for activation culture in step (1), nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are connect Planting in fermentation medium prepared by step (2), amplification culture 48 hours under the rotating speed of 200rpm, cultivation temperature is 32 DEG C;
(4) colloid obtained is organic waste deodorizer.
Comparative example 2
A kind of organic waste deodorizer, according to the raw material of weight portion be: wormcast 100 parts, bacillus subtilis 1.5 parts.Its Preparation method, concretely comprises the following steps:
(1) bacillus subtilis is carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 150g/L, is placed in and there is stirring and function of temperature control In fermentation tank;
(3) in fermentation medium prepared by bacillus subtilis strain good for activation culture in step (1) to step (2), Amplification culture 48 hours under the rotating speed of 200rpm, cultivation temperature is 32 DEG C;
(4) colloid obtained is organic waste deodorizer.
Comparative example 3
A kind of organic waste deodorizer, according to the raw material of weight portion be: wormcast 100 parts, nitrite bacteria 1.5 parts, sulfur are thin Bacterium 1.5 parts, thiobacillus denitrificans 1.5 parts.Its preparation method, concretely comprises the following steps:
(1) respectively nitrite bacteria, sulfur bacteria, thiobacillus denitrificans, phosphobacteria are carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 150g/L, is placed in and there is stirring and function of temperature control In fermentation tank;
(3) nitrite bacteria good for activation culture in step (1), sulfur bacteria, thiobacillus denitrificans, phosphobacteria are inoculated into step Suddenly, in the fermentation medium that prepared by (2), amplification culture 48 hours under the rotating speed of 200rpm, cultivation temperature is 32 DEG C;
(4) colloid obtained is organic waste deodorizer.
Comparative example 4
A kind of organic waste deodorizer, according to the raw material of weight portion be: wormcast 100 parts, EM compound bacteria 6 parts.Its preparation side Method, concretely comprises the following steps:
(1) EM compound bacteria is carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 150g/L, is placed in and there is stirring and function of temperature control In fermentation tank;
(3) EM compound bacteria good for activation culture in step (1) is inoculated in fermentation medium prepared by step (2), Amplification culture 48 hours under the rotating speed of 200rpm, cultivation temperature is 32 DEG C;
(4) colloid obtained is organic waste deodorizer.
Comparative example 5
A kind of organic waste deodorizer, is prepared as wormcast the colloid that mass concentration is 150g/L and is organic waste deodorizer.
Contrast experiment: use organic waste deodorizer based on wormcast and complex microorganism prepared by embodiment and comparative example at rubbish Deodorization Experiment is done in rubbish field.
First select 10 garbage chutes (150 ton day left and right quantity of refuse) to test, be divided into two groups: experimental group and contrast comparison Group, often 5 garbage chutes of group.Experimental group: continuous 7 days, the rubbish at 5 garbage chutes transmits mouth respectively with spray pattern (to the greatest extent Amount is uniformly) throw in dilute with water after the organic waste deodorizer based on wormcast Yu complex microorganism prepared of embodiment 1-5. Contrast matched group: continuous 7 days, the rubbish at 5 garbage chutes transmits mouth respectively with spray pattern (the most uniform) input water Organic waste deodorizer prepared by comparative example 1-5 after dilution.
The compost of organic waste produces and carries out fully according to soot common process.Through the experiment of a cycle (7 days), two The specific experiment result of group please refer to Tables 1 and 2;Wherein odor concentration uses triangle odor bag method (GB/T14675) to survey Fixed, ammonia uses sodium hypochlorite-salicylic acid spectrophotography (GB/T14679) to measure, and hydrogen sulfide uses MBAS spectrophotometry (GB/T11742) measure, during sampling, rubber tube is imbedded in junk-heap body.Residential block air standard uses " ambient air quality Standard " (GB 3095-2012).
The experimental result (meansigma methods) of 1 liang of table group
The experimental result (contrast table) of 2 liang of table group
Experimental result:
Experimental group: rubbish is after 1-2 days, and temperature is rapidly increased to 40-50 DEG C (ambient temperature is 6-8 DEG C), fermentation quickly, is being opened After beginning 20 hours, percolate starts to ooze out, and garbage odor straight line drops to basic odorless;And from Tables 1 and 2, After process, the concentration of ammonia, hydrogen sulfide and sulfur dioxide all can meet residential block air standard, and clearance is up to more than 95%;Rubbish Rubbish heap high sedimentation rate reaches more than 60%.
Contrast matched group: rubbish to start to warm up after 2-3 days, and temperature is 35-45 DEG C (ambient temperature is 6-8 DEG C), stink Reduce but the biggest, and from Tables 1 and 2, after process, the concentration of ammonia, hydrogen sulfide and sulfur dioxide all can not expire Foot residential block air standard, clearance is all below 91%, and major part is about 80%;Its rubbish fermentation is not thorough, and heap height sinks Fall rate is about 45%.
Conclusion: in the present invention, beneficial microbe is with wormcast as growth promoter, obtain is based on wormcast and complex microorganism The harmful substance of the tool foul smell such as organic waste deodorizer decomposable asymmetric choice net ammonia, amine salt, sulfide, based on wormcast and complex microorganism Organic waste deodorizer in the mutual synergism of other materials such as microbial inoculum, do not only reached the purpose of decrement deodorization, it is also possible to will Organic waste is turned waste into wealth, and produces biological organic fertilizer.The present invention is used for organic waste deodorization, decrement, compared to existing technology, raw Producing investment little, production cost is low, pure environmental protection, process effect height, wide adaptability, easy to use.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, at this In the ken that the those of ordinary skill in field is possessed, it is also possible to make various change on the premise of without departing from this patent objective Change.

Claims (5)

1. an organic waste deodorizer based on wormcast Yu complex microorganism, it is characterised in that be according to the raw material of weight portion: wormcast 80-120 part, bacillus subtilis 1-2 part, nitrite bacteria 1-2 part, sulfur bacteria 1-2 part, thiobacillus denitrificans 1-2 part;Its preparation method, concretely comprises the following steps:
(1) respectively bacillus subtilis, nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are carried out activation culture;
(2) first wormcast is prepared as the fermentation medium that mass concentration is 120-180g/L, is placed in the fermentation tank with stirring and function of temperature control;
(3) bacillus subtilis good for activation culture in step (1), nitrite bacteria, sulfur bacteria, thiobacillus denitrificans are inoculated in fermentation medium prepared by step (2), amplification culture 24-72 hour under the rotating speed of 180-220rpm, cultivation temperature is 28-38 DEG C;
(4) colloid obtained is organic waste deodorizer based on wormcast Yu complex microorganism.
Organic waste deodorizer based on wormcast Yu complex microorganism the most according to claim 1, it is characterized in that, according to the raw material of weight portion be: wormcast 90-110 part, bacillus subtilis 1.4-1.6 part, nitrite bacteria 1.4-1.6 part, sulfur bacteria 1.4-1.6 part, thiobacillus denitrificans 1.4-1.6 part.
Organic waste deodorizer based on wormcast Yu complex microorganism the most according to claim 1, it is characterized in that, according to the raw material of weight portion be: wormcast 100 parts, bacillus subtilis 1.5 parts, nitrite bacteria 1.5 parts, sulfur bacteria 1.5 parts, thiobacillus denitrificans 1.5 parts.
4. according to the organic waste deodorizer based on wormcast Yu complex microorganism one of claim 1-3 Suo Shu, it is characterised in that wormcast is prepared as the fermentation medium that mass concentration is 145-155g/L by step (2).
5. according to the organic waste deodorizer based on wormcast Yu complex microorganism one of claim 1-3 Suo Shu, it is characterised in that in step (3) under the rotating speed of 200rpm amplification culture 36-48 hour, cultivation temperature is 32-35 DEG C.
CN201510484089.8A 2015-08-03 2015-08-03 A kind of organic waste deodorizer based on wormcast Yu complex microorganism Expired - Fee Related CN105152700B (en)

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CN109529598A (en) * 2018-11-02 2019-03-29 山东博华高效生态农业科技有限公司 A kind of biological deodorant and preparation method thereof
CN109331209A (en) * 2018-11-13 2019-02-15 宁夏大学 The preparation method of food waste deodorization agent
CN110591959A (en) * 2019-09-28 2019-12-20 成都市锦鑫汇生物科技有限公司 Proteus mirabilis preparation for treating domestic sludge and preparation method and application thereof
CN110591960A (en) * 2019-09-28 2019-12-20 成都市锦鑫汇生物科技有限公司 Hafnia alvei preparation for treating domestic sludge and preparation method and application thereof
CN110591961A (en) * 2019-09-28 2019-12-20 成都市锦鑫汇生物科技有限公司 Composite biological agent for treating domestic sludge and preparation method and application thereof
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