CN110591959A - Proteus mirabilis preparation for treating domestic sludge and preparation method and application thereof - Google Patents
Proteus mirabilis preparation for treating domestic sludge and preparation method and application thereof Download PDFInfo
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- CN110591959A CN110591959A CN201910928204.4A CN201910928204A CN110591959A CN 110591959 A CN110591959 A CN 110591959A CN 201910928204 A CN201910928204 A CN 201910928204A CN 110591959 A CN110591959 A CN 110591959A
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- preparation
- proteus mirabilis
- domestic sludge
- culture medium
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/12—Treatment of sludge; Devices therefor by de-watering, drying or thickening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a proteus mirabilis preparation for treating domestic sludge as well as a preparation method and application thereof, belonging to the technical field of domestic sludge treatment.
Description
Technical Field
The invention belongs to the technical field of domestic sludge treatment, and particularly relates to a proteus mirabilis preparation for treating domestic sludge, and a preparation method and application thereof.
Background
The treatment technology of urban domestic sludge is always an elbow stopping problem which troubles the development of cities. Along with the development of urbanization and the increasing of urban population, the discharge amount of industrial wastewater and domestic sewage is increasing day by day, the annual sewage discharge of China reaches 18 billions of cubic meters, and the sludge generated in the sewage treatment process is calculated by 0.3-0.5 percent of the sewage treatment amount, and the generation amount also reaches thousands of tons. At present, most of domestic large and medium-sized cities comprise Chengdu, Shanghai, Guangzhou, Beijing and the like face the condition of no land burying, and are one of the most outstanding problems facing city construction and environmental protection in recent years. The sludge has complex components, contains toxic, harmful and difficultly-degradable organic matters, pathogenic bacteria, parasites and heavy metals, also contains a large amount of nutrient components necessary for the growth of plants, and has good yield-increasing effect on the growth of crops, so the discharge of untreated sludge can cause serious pollution to the environment and loss of useful components in the sludge. The selectable sustainable treatment and disposal process mainly comprises sludge land utilization, sludge incineration and building material utilization.
In the aspect of land utilization, although the sludge of domestic sewage plants has low heavy metal content, the salt, pathogens and organic pollutants (including hormones, antibiotics and plasticizers) in the sludge pollute soil, surface water and underground water to cause secondary pollution, and in addition, the market acceptance of the sludge products entering the farmland for utilization is low, and the sludge products are not permitted to enter the farmland for utilization in the clear text of China, so certain environmental risks exist in the land agriculture. The urban landscaping and land improvement are common in China, but the consumption of the urban landscaping and land improvement is limited.
The utilization of the sludge building materials is relatively widespread, such as brick making, cement manufacturing, ceramsite manufacturing and fiber board manufacturing, but the utilization of the sludge building materials is limited by local or industrial landscape and is not stable.
The sludge can be incinerated independently or mixed with garbage, industrial boilers and thermal power plants, and is relatively flexible. Particularly, the amount of the mixed burning with garbage and a thermal power plant is more, and the amount of the single burning is relatively less. The single incineration is not suitable for projects with small sludge scale, the incineration of the sludge is popular with people once, however, the sludge incineration is limited in use by large one-time investment, high equipment investment, high operation cost, generation of dioxin and other highly toxic substances when the combustion temperature of organic matters is lower than 850 ℃, treatment of flue gas, retreatment of incineration ash and the like.
In addition, in the existing domestic and foreign sludge treatment modes, the drying mostly adopts the forms of heat drying and composting, although methods of optimizing heat sources, reducing process steps, optimizing operation parameters and the like are adopted to reduce energy consumption, the process must utilize an external heating source, so that the development of the heat drying process is limited.
Disclosure of Invention
The invention aims to: aiming at the problems in the prior art, the proteus mirabilis preparation for treating domestic sludge and the preparation method and application thereof are provided, the proteus mirabilis preparation has a good removing effect on the peculiar smell and the water content of the sludge, does not need an external heating source, is environment-friendly, energy-saving, economical and reliable, is convenient to treat, and can convert the sludge into a soil conditioner which has a high organic value, no peculiar smell and stable properties.
The technical scheme adopted by the invention is as follows:
a preparation method of a proteus mirabilis preparation for treating domestic sludge comprises the following steps:
s1, soaking fresh earthworm cast in water for 30-40min, and taking a soaking solution;
s2, adding the soak solution obtained in the step S1, proteus mirabilis strains and a culture medium into a culture container, and culturing at the temperature of 12-15 ℃ for 11-13h to obtain a culture mother solution;
and S3, adding a culture medium into the culture mother liquor obtained in the step S2, and culturing at normal temperature for 11-13h to obtain the culture medium.
The principle of treating the domestic sludge by adopting the method is to simulate the metabolic mode of the earthworms, and the proteus mirabilis in the earthworm metabolites is obviously polymorphic, has spherical and filamentous shapes, is periflagellate and is active in movement. The growth is diffused on the solid culture medium, and the phenomenon of migratory growth is formed. If 0.1% carbolic acid or 0.4% boric acid is added into the culture medium, the diffusion growth of the carbolic acid or boric acid can be inhibited, and a common single colony is formed. Can decompose urea rapidly. The proteus mirabilis has the functions of rapidly metabolizing water in the sludge and breaking the constraint of a flocculating agent and the like, so that the sludge is converted into a soil conditioner which has high organic value, no peculiar smell and stable properties. After the microorganisms are propagated in a large amount under a proper culture method and culture conditions, sufficient ventilation and oxygen supply can be ensured under the condition of a temperature much lower than that of an aerobic composting method and a biological drying method, and sludge with the water content of about 80 percent is converted into a soil conditioner with the water content of less than 60 percent in 10 to 15 days.
The invention mainly comprises two stages for treating domestic sludge: 1) mainly adopts microbial inoculum treatment. The microbial inoculum metabolizes water in the sludge, stabilizes organic matters, breaks the constraint of a flocculating agent and releases bound water. 2) Mainly natural evaporation. After the structure of the binding water of the flocculant of the sludge is broken, the sludge becomes loose and porous, and the water is removed by natural evaporation.
Further, the ratio of the wormcast to the water in the step S1 is 0.38-0.50 g: 1 mL.
Further, the culture medium in the steps of S2 and S3 comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Further, the volume ratio of the culture medium, the soak solution and the proteus mirabilis strain in the step S2 is 5-5.5: 0.1-0.15: 0.01-0.015.
Further, the volume ratio of the culture medium, the soak solution and the proteus mirabilis strain in the step S2 is 5:0.1: 0.01.
further, the volume ratio of the culture medium to the culture mother liquor in the step S3 is 10-10.5: 0.2-0.25.
Further, the volume ratio of the medium to the culture mother liquor in the step S3 was 10: 0.2.
The proteus mirabilis preparation for treating the domestic sludge is prepared by the preparation method.
The proteus mirabilis preparation is applied to treatment of domestic sludge.
The application specifically includes: uniformly spraying the proteus mirabilis preparation on domestic sludge, uniformly turning over, and keeping ventilation in the domestic sludge treatment process for 10-15 days; the volume ratio of the proteus mirabilis preparation to the domestic sludge is 1: 2.5.
in summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. according to the method, the culture mode and the operation flow of proteus mirabilis are simple and easy to operate, the wormcast soak solution is adopted for culture, raw materials are easy to obtain, microorganisms in the wormcast and the proteus mirabilis used in the method play a synergistic effect in the treatment process of the domestic sludge, the constraint of a flocculating agent is broken, and moisture in the sludge is rapidly metabolized;
2. the proteus mirabilis preparation prepared by the invention has no secondary pollution when being used for treating the domestic sludge, the obtained soil has high organic value, no peculiar smell and stable properties, and can be used for gardens, land agriculture and the like;
3. the treatment period is short, only 10-15 days are needed, and the sticky sludge with the water content of about 80% can be converted into the odorless loose soil with the water content of less than 60%;
4. the method of the invention for treating the domestic sludge meets the requirements of national resource sustainable development and has low treatment cost, so that the product has great market advantages.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The preparation method of the proteus mirabilis preparation for treating domestic sludge provided by the preferred embodiment of the invention comprises the following specific steps:
s1, soaking fresh wormcast in water for 30min, taking a soaking solution, wherein the ratio of wormcast to water is 0.38 g: 1 mL;
s2, adding the soaking solution obtained in the step S1, proteus mirabilis strains and a culture medium into a culture container according to the volume ratio of the culture medium to the soaking solution to the proteus mirabilis strains of 5:0.1:0.01, and culturing at the temperature of 12 ℃ for 12 hours to obtain a culture mother solution;
s3, dispersing the culture mother liquor obtained in the step S2 in a culture bucket, adding a culture medium into the culture bucket, wherein the volume ratio of the culture medium to the culture mother liquor is 10:0.2, and culturing at normal temperature for 12 hours to obtain the culture medium.
Wherein the culture medium is a commercial culture medium and comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Example 2
The preparation method of the proteus mirabilis preparation for treating domestic sludge provided by the preferred embodiment of the invention comprises the following specific steps:
s1, soaking fresh wormcast in water for 30min, taking a soaking solution, wherein the ratio of wormcast to water is 0.50 g: 1 mL;
s2, adding the soaking solution obtained in the step S1, the proteus mirabilis strain and the culture medium into a culture container according to the volume ratio of the culture medium to the soaking solution to the proteus mirabilis strain being 5.2:0.1:0.01, and culturing at the temperature of 13 ℃ for 12 hours to obtain a culture mother solution;
s3, dispersing the culture mother liquor obtained in the step S2 in a culture bucket, adding a culture medium into the culture bucket, wherein the volume ratio of the culture medium to the culture mother liquor is 10.2:0.2, and culturing at normal temperature for 12 hours to obtain the culture medium.
Wherein the culture medium is a commercial culture medium and comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Example 3
The preparation method of the proteus mirabilis preparation for treating domestic sludge provided by the preferred embodiment of the invention comprises the following specific steps:
s1, soaking fresh wormcast in water for 30min, taking a soaking solution, wherein the ratio of wormcast to water is 0.45 g: 1 mL;
s2, adding the soaking solution obtained in the step S1, the proteus mirabilis strain and the culture medium into a culture container according to the volume ratio of the culture medium to the soaking solution to the proteus mirabilis strain being 5.5:0.1:0.01, and culturing at 15 ℃ for 12 hours to obtain a culture mother solution;
s3, dispersing the culture mother liquor obtained in the step S2 in a culture bucket, adding a culture medium into the culture bucket, wherein the volume ratio of the culture medium to the culture mother liquor is 10.5:0.2, and culturing at normal temperature for 12 hours to obtain the culture medium.
Wherein the culture medium is a commercial culture medium and comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Example 4
The preparation method of the proteus mirabilis preparation for treating domestic sludge provided by the preferred embodiment of the invention comprises the following specific steps:
s1, soaking fresh wormcast in water for 30min, taking a soaking solution, wherein the ratio of wormcast to water is 0.42 g: 1 mL;
s2, adding the soaking solution obtained in the step S1, the proteus mirabilis strain and the culture medium into a culture container according to the volume ratio of the culture medium to the soaking solution to the proteus mirabilis strain being 5.3:0.1:0.01, and culturing at 14 ℃ for 12 hours to obtain a culture mother solution;
s3, dispersing the culture mother liquor obtained in the step S2 in a culture bucket, adding a culture medium into the culture bucket, wherein the volume ratio of the culture medium to the culture mother liquor is 10.3:0.2, and culturing at normal temperature for 12 hours to obtain the culture medium.
Wherein the culture medium is a commercial culture medium and comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Example 5
The preparation method of the proteus mirabilis preparation for treating domestic sludge provided by the preferred embodiment of the invention comprises the following specific steps:
s1, soaking fresh wormcast in water for 30min, taking a soaking solution, wherein the ratio of wormcast to water is 0.48 g: 1 mL;
s2, adding the soaking solution obtained in the step S1, proteus mirabilis strains and a culture medium into a culture container according to the volume ratio of the culture medium to the soaking solution to the proteus mirabilis strains of 5:0.12:0.01, and culturing at the temperature of 12 ℃ for 12 hours to obtain a culture mother solution;
s3, dispersing the culture mother liquor obtained in the step S2 in a culture bucket, adding a culture medium into the culture bucket, wherein the volume ratio of the culture medium to the culture mother liquor is 10:0.21, and culturing at normal temperature for 12 hours to obtain the culture medium.
Wherein the culture medium is a commercial culture medium and comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Example 6
The preparation method of the proteus mirabilis preparation for treating domestic sludge provided by the preferred embodiment of the invention comprises the following specific steps:
s1, soaking fresh wormcast in water for 30min, taking a soaking solution, wherein the ratio of wormcast to water is 0.40 g: 1 mL;
s2, adding the soaking solution obtained in the step S1, the proteus mirabilis strain and the culture medium into a culture container according to the volume ratio of the culture medium to the soaking solution to the proteus mirabilis strain being 5.1:0.13:0.01, and culturing at the temperature of 13 ℃ for 12 hours to obtain a culture mother solution;
s3, dispersing the culture mother liquor obtained in the step S2 in a culture bucket, adding a culture medium into the culture bucket, wherein the volume ratio of the culture medium to the culture mother liquor is 10.2:0.23, and culturing at normal temperature for 12 hours to obtain the culture medium.
Wherein the culture medium is a commercial culture medium and comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
Examples of the experiments
The domestic sludge was treated with the proteus mirabilis preparation prepared in example 1, and index parameters before and after the treatment of the domestic sludge were measured.
The processing method comprises the following steps: uniformly spraying the proteus mirabilis preparation on domestic sludge and uniformly turning over, wherein the volume ratio of the proteus mirabilis preparation to the domestic sludge is 1: 2.5, keeping ventilation in the domestic sludge treatment process, and treating for 12 d.
TABLE 1 index parameters before and after treatment of domestic sludge
| Water content (%) | Smell(s) | Form of the composition | |
| Before treatment | 80 | Strong ammonia and hydrogen sulfide odor | Grey black, viscous jelly-like |
| After treatment | ＜60 | Has no peculiar smell | Yellow, loose and loose |
As can be seen from the above Table 1, the domestic sludge treated by the preparation and the method of the present invention has great differences in moisture content, appearance and odor from the domestic sludge before treatment, and has no peculiar smell, loose form and low moisture content, which indicates that the preparation of the present invention has good effect in treating the domestic sludge.
Comparative example
The domestic sludge is treated by 4 different methods of the proteus mirabilis preparation prepared in the embodiment 1 of the invention, incineration, composting and biological drying respectively, and the results are shown in the following table 2:
table 2 comparative experiments and related data
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A preparation method of a proteus mirabilis preparation for treating domestic sludge is characterized by comprising the following steps:
s1, soaking fresh earthworm cast in water for 30-40min, and taking a soaking solution;
s2, adding the soak solution obtained in the step S1, proteus mirabilis strains and a culture medium into a culture container, and culturing at the temperature of 12-15 ℃ for 11-13h to obtain a culture mother solution;
and S3, adding a culture medium into the culture mother liquor obtained in the step S2, and culturing at normal temperature for 11-13h to obtain the culture medium.
2. The method for preparing the proteus mirabilis preparation for treating domestic sludge according to claim 1, wherein the method comprises the following steps: the ratio of the wormcast to the water in the step S1 is 0.38-0.50 g: 1 mL.
3. The method for preparing the proteus mirabilis preparation for treating domestic sludge according to claim 1, wherein the method comprises the following steps: the culture medium in the S2 and S3 steps comprises the following components in a mass ratio of 4: 1: 1: 1: 1000 of beef extract, corn flour, peptone, sodium chloride and water.
4. The method for preparing the proteus mirabilis preparation for treating domestic sludge according to claim 3, wherein the step of preparing the proteus mirabilis preparation comprises the following steps: the volume ratio of the culture medium, the soak solution and the proteus mirabilis strain in the step S2 is 5-5.5: 0.1-0.15: 0.01-0.015.
5. The method for preparing the proteus mirabilis preparation for treating domestic sludge according to claim 4, wherein the step of preparing the proteus mirabilis preparation comprises the following steps: the volume ratio of the culture medium, the soak solution and the proteus mirabilis strain in the step S2 is 5:0.1: 0.01.
6. the method for preparing the proteus mirabilis preparation for treating domestic sludge according to claim 3, wherein the step of preparing the proteus mirabilis preparation comprises the following steps: the volume ratio of the culture medium to the culture mother liquor in the step S3 is 10-10.5: 0.2-0.25.
7. The method for preparing a proteus mirabilis preparation for treating domestic sludge according to claim 6, wherein the step of preparing the proteus mirabilis preparation comprises the following steps: the volume ratio of the culture medium to the culture mother liquor in the step S3 is 10: 0.2.
8. Proteus mirabilis preparation for treating domestic sludge prepared by the preparation method of any one of claims 1-7.
9. Use of the proteus mirabilis preparation according to claim 8 for the treatment of domestic sludge.
10. The use according to claim 9, characterized in that it is in particular: uniformly spraying the proteus mirabilis preparation on domestic sludge, uniformly turning over, and keeping ventilation in the domestic sludge treatment process for 10-15 days; wherein the volume ratio of the proteus mirabilis preparation to the domestic sludge is 1: 2.5.
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| CN201910928204.4A CN110591959A (en) | 2019-09-28 | 2019-09-28 | Proteus mirabilis preparation for treating domestic sludge and preparation method and application thereof |
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| CN201910928204.4A CN110591959A (en) | 2019-09-28 | 2019-09-28 | Proteus mirabilis preparation for treating domestic sludge and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151052A (en) * | 2021-03-15 | 2021-07-23 | 西安石油大学 | Oil-containing sludge degrading strain Proteus mirabilis SB and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070203A (en) * | 2007-06-18 | 2007-11-14 | 同济大学 | Method for preparing biological flocculant using strange deformation bacilli |
| CN106220259A (en) * | 2015-08-03 | 2016-12-14 | 刘雷 | A kind of organic waste deodorizer based on wormcast Yu complex microorganism |
| CN108609743A (en) * | 2018-05-22 | 2018-10-02 | 惠州市兴牧环保科技股份有限公司 | A method of utilizing microbial degradation ammonia nitrogen |
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2019
- 2019-09-28 CN CN201910928204.4A patent/CN110591959A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070203A (en) * | 2007-06-18 | 2007-11-14 | 同济大学 | Method for preparing biological flocculant using strange deformation bacilli |
| CN106220259A (en) * | 2015-08-03 | 2016-12-14 | 刘雷 | A kind of organic waste deodorizer based on wormcast Yu complex microorganism |
| CN108609743A (en) * | 2018-05-22 | 2018-10-02 | 惠州市兴牧环保科技股份有限公司 | A method of utilizing microbial degradation ammonia nitrogen |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151052A (en) * | 2021-03-15 | 2021-07-23 | 西安石油大学 | Oil-containing sludge degrading strain Proteus mirabilis SB and application thereof |
| CN113151052B (en) * | 2021-03-15 | 2022-06-17 | 西安石油大学 | Oil-containing sludge degrading strain Proteus mirabilis SB and application thereof |
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Application publication date: 20191220 |