WO2003093454A1

WO2003093454A1 - A microbe preparation for treating waste alcohol, its preparation method and the fermentation process

Info

Publication number: WO2003093454A1
Application number: PCT/CN2003/000224
Authority: WO
Inventors: Biu Ho; Enhsiung Huang
Original assignee: Gaoming Jinkuizi Plant Nutriment Co.,Ltd.
Priority date: 2002-04-30
Filing date: 2003-03-28
Publication date: 2003-11-13
Also published as: CN1377958A; AU2003227171A1

Abstract

The application disclosed a microbe preparation for treating waste alcohol from distillery and waste molasses from sugar refinery features that the WH flora is used as microbe flora. The preparation method and fermentation process is also described. The fermentation process comprising, a first fermentation process carried out by adding the microbe preparation into the waste alcohol, and then the organic sludge is fermented. The two fermented products are mixed together and subjected to further fermentation, thus the organic manure is obtained. Its advantages are simple process and low cost. This method can be used to solve some environmental problems.

Description

Microbial fermentation agent for treating alcohol waste liquid, preparation method and fermentation process thereof

The invention relates to the technical field of alcohol waste liquid treatment, in particular to a microorganism fermentation agent for treating alcohol waste liquid, a preparation method thereof and a fermentation process. Background technique

China currently has more than 400 gansu sugar factories. Waste molasses, a by-product of sugar production, produces alcohol through an alcohol plant. Each lm ³ alcohol produced requires 20 m ^{3 of} waste alcohol liquor. The annual output is 25,000 m ^{3 of} alcohol, which requires 500,000. m ³ liquor liquor waste. Alcohol waste liquid contains high concentrations of COD (chemical oxygen consumption), BOD (biological oxygen consumption) and SS (suspended matter), which are directly discharged and cause serious pollution to the local ecological environment. For a long time, a lot of funds, manpower and material resources have been invested to deal with the problem of alcohol waste liquid. So far, there is no better treatment plan.

The current methods for treating alcohol waste are:

(1) Direct discharge method: This method is suitable for non-sandy farmland with large areas outside the plant that can withstand wastewater purification and lack water. However, waste liquid such as seepage into rivers or groundwater sources can cause pollution.

(2) Alcohol waste liquid concentration method: After the multi-effect evaporation system is used to concentrate the alcohol waste liquid, it is processed into a powder by high-temperature spray treatment before it can be used. The equipment investment is large and other costs are also large. The powder after treatment is easy to absorb moisture and agglomerate.

(3) Natural microorganism fermentation treatment method: Recently, there is a method for treating alcohol waste liquid by using microorganisms existing in the natural world, which has four steps:

①Using high temperature fermentation: Install a large-scale fermentation tank with a blower, aeration aerobic fermentation, add natural microorganisms to carry bacteria from the material and ferment in the fermentation tank for 7-12 days;

② Adjust the mixed fermentation: add the above-mentioned materials to the fermentation process containing the mold, and increase the PH value of the fermentation process, the carbon-nitrogen ratio is out of balance, add the acid-base regulator, the carbon-nitrogen ratio regulator, and ferment in 3-4 days;

③ Turn over the materials obtained in the above step ②, add water, control the temperature, and ferment for 20-30 days;

④ Add the material obtained in steps ③ and ③ to a substance rich in soil active microorganisms, and ferment the soil active microorganisms for 8-14 days.

After the above ①-④ stages of fermentation, the time is up to 60 days, and finally chemical fertilizer is added to become alive. Sexual organic compound fertilizer. This method is better than the first two, but the process is complicated, the fermentation time is up to 60 days, multiple large-scale fermentation tanks with ventilated exhaust equipment need to be built, and natural active microorganisms and mold starters need to be collected, which takes a lot of labor , Financial resources, and time. Summary of the Invention

An object of the present invention is to provide a microbial flora capable of treating alcohol waste liquid.

Another object of the present invention is to provide a microbial fermentation agent for treating alcohol waste liquid, which can quickly and efficiently ferment alcohol waste liquid into a raw material of active organic fertilizer.

Another object of the present invention is to provide a method for preparing the fermentation agent.

Another object of the present invention is to provide a fermentation process for treating alcohol liquor using the fermentation agent. The microbial flora for treating alcohol waste liquor provided by the present invention comprises: Bacillus polymyxa CGMCC 0395.1, named 1 species ; Bacillus subtilis CGMCC 0395.2, named species 2; Bacillus brevis CGMCC 0395.3, named species 3; Bacillus licheniformis CGMCC 0395.4, named species WH4; Brevibacterium thiochroman CGMCC 0395.5, named species 5 Bacteria. The main components of the microbial fermentation agent for treating alcohol waste liquid provided by the present invention include a microbial flora and a culture medium composed of the above five kinds of bacteria.

The culture medium preferably has the following formula: (weight percentage) rice bran: 70-80, soybean meal: 10 -20, milk meal: 2-5, meat and bone meal: 0.1- 0.2, fish bone meal: 0.1- 0.2, sugar: 1- 3. Water: 1-2.

The microbial fermentation agent is further preferred, and the proportion of the strains contained in the microbial flora is (weight percentage): Li 1 strain: 20-35, Li 2 strain: 5-20, TO3 strain: 15 — 30, Li 4 strains: 8—15, WH5 strains: 22—30. The method for preparing the microbial fermentation agent provided by the present invention includes the following steps:

(1) Preparation of flora: WH1 strains, H2 strains, 3 strains, WH4 strains, and 5 strains are mixed to form a WH flora;

(2) Formula of secondary culture medium (weight percentage): Rice bran: 70-80 Soy flour: 10 -20 Milk powder: 2- 5 Meat and bone meal: 0.1-

0.2

Fish bone meal: 0.1- 0.2 sugar: 0.8-3 water: 1-2;

(3) Secondary strain culture: Firstly mix the secondary strain culture medium according to the above formula and mix the hooks, inoculate the book flora at normal temperature, pile up and cover, the temperature starts to rise, and the temperature reaches 35 ° C-40 after 2 days. ° C, after opening the cover for 30 minutes for natural heat dissipation every day, cover it for 8-10 days, and obtain the secondary active furfur bacteria of WH flora, which will become a microbial fermentation agent for treating alcohol waste liquid.

In this method, preferably, the proportion of the bacteria contained in the microbial flora in step (1) is (weight percentage): Book 1 bacteria: 20—35, 2 bacteria: 5-20, WH3 bacteria: 15—30 , Book 4 strains: 8-15, WH5 strains: 22-30. The method for treating alcohol waste liquid by the microbial fermentation agent provided by the present invention includes the following steps:

(1) Tertiary strain culture: use alcohol waste liquid as tertiary strain culture medium;

Fermentation process of album bacteria: Add the microbial fermentation agent to the alcohol waste liquid, stir evenly, and inflate intermittently, three times a day, 15-30 minutes each time, the temperature is 24 ° C-38 ° C, PH value 3.5 -7.5, after 10-14 days, at least the surface foam of the liquid will increase, the liquid will change from thick to thin, the color will change from dark brown to brown, and the hammer will decrease from 50-60 degrees at the beginning to 43-46 degrees, that is, the fermentation is complete. ;

(2) Filter sludge fermentation: transport fresh filter sludge to the open space of the drying field and spread it at a height of 40-50 cm. At the same time, spray the microbial fermentation agent for treating alcohol waste liquid onto the filter sludge, mix it with a hook, and bury it to cover. To allow it to ferment, hyphae will appear after 2 to 3 days, and the temperature will reach 55 ° C-60 ° C. The piles will be turned every 5-7 days, and the fermentation will be completed after 15 days.

(3) Comprehensive fermentation: Steps (1) and (2) of step J are performed simultaneously. After completion, the fermented alcohol waste liquid obtained in step (1) and the fermented filter mud obtained in step (2) are pressed as follows: 2.5—3 (weight ratio) Mixed, stirred and fermented, the temperature is between 4 (rC_5 (TC), and it will become the active organic fertilizer raw material after turning over the heat and fermenting and drying. Compared with the prior art, the present invention has significant social benefits. Ecological and economic benefits:

① The use of the microbial fermentation agent of the present invention can completely solve the three waste problems of sugar factories and alcohol factories, which is beneficial to production, environmental protection and the relationship between factories and groups.

② The production process is short and can be completed in one month. The process is simple, the cost is low, and the investment is small. No large-scale equipment is required, and the process of the present invention can be completed only in a single cement tank.

③After comprehensive fermentation, the wastes such as alcohol liquor and filter sludge are turned into wastes, and raw materials for the production of active organic fertilizers are added to increase economic benefits for the factory. Best Mode for Carrying Out the Invention The present invention is further explained in detail through the following examples.

Example 1:

1. The microbial flora of the microbial fermentation agent for the treatment of alcoholic waste liquid is composed of Bacillus polymyxa CGMCC 0395.1, named TO1 strain; Bacillus subtilis CGMCC 0395.2, named WH2 strain; Bacillus brevis CGMCC0395. 3, named the 3 species of bacteria; Bacillus licheniformis CGMCC 0395. 4, named WH4 strains; and Brevibacterium thiochroman CGMCC 0395. 5, named Li 5 strains to form the Li flora.

The proportion of the above microbiome is (weight percent):

WH1 strain: 20 WH2 strain: 20

Issue 3 strains: 22 4 strains: 12

WH5 strains: 26.

2. Microbial preparation 1. Preparation method of alcohol fermentation liquid fermentation agent:

(1) Formulating the albumen flora, the albumen bacteria 1, album 2 bacteria, WH3 bacteria, WH4 bacteria, WH5 bacteria are mixed according to the above formula to form a WH flora;

(2) The medium for secondary bacteria culture is prepared according to the following formula (weight percentage): rice bran: 80 soy flour: 16 milk powder: 2 meat and bone meal: 0.1 fish bone meal: 0.1 sugar: 0.8 water: 1 ;

The prepared secondary bacteria culture medium was inoculated with the above-mentioned TO flora at normal temperature, and white hyphae appeared on the medium the next day. The culture temperature rose to 35 ° C, the pH value was 7.0, naturally radiated, and turned once after hardening. The secondary active mycoplasma of WH flora was obtained within 10 days, which is the alcohol fermentation broth.

3. A method for treating alcohol waste liquid with a microbial fermentation agent, comprising the following steps:

(1) Tertiary bacteria culture, using alcohol waste liquid as the tertiary medium, the average content (weight percentage) of the main components of the alcohol waste liquid:

Organic matter: 7. 5 Sugar: 3.4 N: 0.3 P: 0.02

K: 0.8 Ca: 0.3 Mg: 0.3; After transferring the alcohol waste liquid to the fermentation tank, adding the adjusted fermenting agent and stirring, the gas was intermittently pumped three times a day for 15 minutes each time at a temperature of 38 ° C and a pH value of 3.5. After 12 days, the foam on the surface of the fermentation liquid was increased. at least, by the thinning thick liquid, color from dark brown to pale Gar, from 50 ° Brix at the start, down to 43 degrees, i.e., completion of the fermentation _d

(2) Filter mud fermentation: transport fresh filter mud to the open space of the drying field and spread it out. The height is 40cm, and the length and width are determined by the size of the drying field. Cover it with thin tape to allow it to ferment naturally. Mycelia appear white, yellow, and green at 2 days after inoculation, and the temperature can reach 55 ° C. The piles of maggots are turned over every 5 days, and the fermentation is completed in 15 days.

(3) Comprehensive fermentation: Mix the alcohol waste liquor and filter sludge that have been fermented in step (1) and (2) at a ratio of 1: 2.5-3 (weight ratio), stir and ferment, and the temperature is 40-50 Between ° C, the filter sludge absorbs the alcohol waste liquid, turns it over to dissipate heat, and dries naturally to become an active organic fertilizer raw material. Example 2:

1. The microbial flora of the microbial fermentation agent for treating alcohol waste liquid is the same as in Example 1, but the ratio of the WH strains of the microbial flora is (weight percentage):

WH1 strain: 28 WH2 strain: 12

WH3 strain: 30, WH4 strain: 8

WH5 strains: 22.

2. Preparation method of alcohol waste liquor starter:

(1) The method of formulating the flora is the same as in Example 1.

(2) The medium for secondary bacteria culture is prepared according to the following formula (weight percentage): rice bran: 70 soy flour: 20 milk powder: 5 meat and bone meal: 0.2

Fish bone meal: 0.2 sugar: 3 water: 1.6;

The prepared secondary bacteria culture medium was inoculated with the above-mentioned bacterial flora at normal temperature. Two days later, white hyphae appeared in the culture medium, and the culture temperature rose to 38 ° C. 9, naturally dissipates heat, flips once after hardening, and cultivates for 9 days to obtain secondary active mycobacterium of WH flora.

3. A method for treating alcohol waste liquid with a microbial fermentation agent, including the following steps:

(1) Tertiary strain culture: using alcohol waste liquid as tertiary strain culture medium;

Transfer the alcohol waste liquid to the fermentation tank, add the adjusted fermenting agent and stir well, and then inflate three times a day for 20 minutes each time, the temperature is 31 ° C, pH 5.5, and the surface of the fermentation liquid will be soaked for 10 days The foam changes from at least, the liquid changes from thick to thin, the color changes from dark to brown, and the hammer degree changes from the beginning

55 degrees to 45 degrees, the fermentation is complete.

(2) Fermentation of filter mud: transport fresh filter mud to the open space of the drying ground and spread it out. The height is 45cm, and the length and width are determined by the size of the drying ground. Cover it with a thin tape to allow it to ferment naturally. Mycelia appear white, yellow, and green at 2 days after inoculation. The temperature can reach 57 ° C. The piles of pupae are turned over every 6 days, and the fermentation is completed in 15 days.

(3) Comprehensive fermentation: Mix the materials that have been fermented in step (1) and (2) according to 1: 2.5 (weight ratio), stir and ferment, the temperature is between 40-50 ° C, and turn over Dissipates heat and becomes raw material for active organic fertilizer after natural drying. Example 3:

WH1 strain: 35 Li 2 strain: 5

WH3 strain: 15 WH4 strain: 15

WH5 strain: 30.

2. Preparation method of alcohol waste liquor starter:

(1) The preparation of WH flora is the same as in Example 1.

(2) The culture medium of the secondary bacteria culture is prepared according to the following formula (weight percentage): rice bran: 75 soy flour: 17 milk powder: 3.1 meat and bone meal: 0.2 fish bone meal: 0.2 sugar: 2.5 Water: 2;

The prepared secondary bacteria culture medium was inoculated with WH flora at normal temperature. Two days later, white hyphae appeared in the culture medium, the culture temperature rose to 40 ° C, PH value was 6. 8, and the heat was released naturally. The secondary active mycoplasma of WH flora was obtained for 8 days.

(1) Tertiary strain culture: using alcohol waste liquid as the tertiary strain culture medium;

The alcohol liquor is transferred to the fermentation tank. After adding the adjusted fermentation agent and stirring, the gas is intermittently pumped three times a day for 30 minutes at a temperature of 24 ° C and a pH value of 7. 5, after 14 days, the surface of the fermentation liquid The foam changes from at least, the liquid changes from thick to thin, the color changes from dark to brown, and the hammer decreases from 60 degrees at the beginning to 46 degrees, that is, the fermentation is complete. ' (2) Filter mud fermentation: transport fresh filter mud to the open space of the drying field and spread it out. The height is 45cm. The length and width are determined by the size of the drying field. Cover it with a thin plastic sheet to allow it to ferment naturally. Hyphae appear white, yellow, and green after 3 days of inoculation, and the temperature can reach 60 ° C. The pile of pupae is turned over every 7 days, and the fermentation is completed in 15 days.

(3) Comprehensive fermentation: The materials fermented in steps (1) and (2) above are mixed in a ratio of 1: 3 (weight ratio), stirred evenly, and then subjected to secondary fermentation immediately. After being naturally dried, it becomes the raw material of active organic fertilizer. . Example 4:

Example of treatment of alcohol concentrated waste liquid in Guangxi Phoenix Sugar Co., Ltd .:

1. Technological process of alcohol liquid concentrate:

The approach is divided into three steps:

① Concentrated alcohol waste liquid is transported from the storage tank to the fermentation tank, and the bacteria flora alcohol waste liquid fermentation agent is added, and the air is intermittently pumped for 15-30 minutes three times a day. The microorganisms multiply and ferment in the tank at a temperature of 24-38 ° C. , PH value 6. 3-6. 6, After 12 days, the surface of the liquid foamed at least, the liquid changed from thick to thin, the color changed from dark to brown, and the hammer decreased from 60 degrees at the beginning to 46 degrees, that is, fermentation carry out.

② Fermentation of filter sludge: Transport fresh filter sludge to the open space in the drying yard, spread it at a height of 40-50 cm, and spray the adjusted alcohol waste liquid leavening agent on the filter sludge evenly with a small pump. After homogenizing, bury the pile, cover it with colored strips, and let it ferment. After 2 to 3 days of inoculated filter mud pile, white and green mycelia gradually form in the pile, and the temperature can reach 55 ° C-60 ° C. After the heap, the fermentation is turned over once in 7 days, and the fermentation is completed in 15 days. The above steps (1) and (2) can be performed simultaneously.

③ Comprehensive fermentation: The fermented mature alcohol waste liquid is sprayed on the fermented filter sludge 2 to 3 times for mixing, stirring, secondary fermentation, and drying. The mixing ratio of the filter sludge and the alcohol concentrated waste liquid is 2.5 to 3 tons. The filter sludge absorbs 1 ton of alcohol waste liquid, and the bagasse ash is added, and the pile is turned according to the fermentation situation, and the fermentation is dried. The fermentation temperature is maintained at 40-50. ° C, after drying, it is the raw material of active organic fertilizer.

2. Analysis of the economic and social benefits of the "three wastes" treatment of sugar plants by biological fermentation:

Guangxi Fenghuang Sugar Factory has expanded the daily production of sugarcane due to the expansion of the production scale of the factory in recent years. In addition to the official production of a large amount of sugar and alcohol, more than four organic waste residues and waste liquid are left. The medium filtration sludge reaches 25 thousand tons, sugar cane ash 5,000 tons, and alcohol waste liquid 12 thousand tons. The discharge of these malodorous waste residue waste liquid seriously affects the living environment of the surrounding residents and also constitutes the location of the Phoenix River. One of the sources of pollution, so cleaning up these wastes and ensuring the cleanliness of the environment has become an urgent problem. The alcohol waste liquid biological fermentation agent of the present invention is used to comprehensively treat the above waste slag waste liquid to turn waste into treasure and turn harm into profit, and produce raw materials capable of producing high-quality active organic fertilizer. This method for comprehensively treating waste residue and waste liquid is easy to implement, requires less investment, and has high benefits. It not only solves environmental protection problems, but also turns waste into treasure, increasing economic and social benefits. Industrial applications! ~ Born

The fermenting agent and the fermentation process for treating alcohol liquid in the present invention can completely solve the three waste problems of sugar factories and alcohol factories. The fermentation process not only has a short process flow, but also has a low process cartridge cost, and has significant social and ecological benefits. Economic benefits.

Claims

Rights request

1. A microbial flora for treating alcohol waste liquid, characterized in that the microbial flora includes: Bacillus polymyxa CGMCC 0395. 1, named TO1 strain; Bacillus subtilis CGMCC 0395. 2, named Li 2 strains; Bacillus brevis CGMCC 0395. 3, named as WH3 strain; Bacillus licheniformis CGMCC 0395. 4, named as WH4 strain; Brevibacterium thiochroman CGMCC 0395. 5, named as WH5 strain.

2. A fermented biological fermentation agent for treating alcohol waste liquid, characterized in that the main components of the microbial fermentation agent are the microbial flora and culture medium according to claim 1.

3. The microbial fermentation agent according to claim 2, characterized in that: the proportion of the bacterial species contained in the microbial flora is (weight percentage):

WH1 strain: 20—35 WH2 strain: 5—20

WH3 strain: 15-30 WH4 strain: 8-15

WH5 strains: 22-30.

4. A method for preparing a microbial fermentation agent according to claim 2, comprising the steps of: (1) preparing a WH flora: a book 1 strain, a WH2 strain, a WH3 strain, a WH4 strain, and a 5 strain Species are mixed to form WH flora;

(2) Formula of secondary culture medium (weight percentage):

Rice Spring: 70-80 Soy flour: 10-20 Milk powder: 2-5 Meat and bone meal: 0.1-

0. 2

Fish bone meal: 0. 1-0. 2 Sugar: 0. 8-3 Water: 1-2;

(3) Secondary strain culture: Firstly mix the secondary strain culture medium according to the above formula and mix the hooks, inoculate Li flora at normal temperature, pile up and cover, the temperature starts to rise, and the temperature reaches 35 ° C— 40 days later ° C, and then open the cover for 30 minutes for natural heat dissipation every day, and then cover it. The culture time is 8-10 days, and the secondary active furfur bacteria of the bacterial flora are obtained, which becomes the microbial fermentation agent for treating alcohol waste liquid.

5. The method for preparing a microbial fermentation agent according to claim 4, characterized in that, in step (1), the ratio of the bacteria contained in the microbial flora is (weight percentage):

WH1 strain: 20—35 WH2 strain: 5—20

WH3 strain: 15-30 WH4 strain: 8-15

WH5 strains: 22-30.

6. A method for treating alcohol waste liquid by using the microbial fermentation agent according to claim 2, comprising: Include the following steps:

Fermentation process of album bacteria: After adding the microbial fermentation agent according to claim 2 to the alcoholic waste liquid and stirring well, intermittently aerated, three times a day, each time 15-30 minutes, the temperature is 24 ° C-38 ° C, PH value 3. 5-7. 5. After 10-14 days of fermentation, the hammer degree decreases from 50-60 degrees at the beginning to 43-46 degrees, that is, the fermentation is completed;

(2) Filter sludge fermentation: transport fresh filter sludge to the open space of the drying field and spread it out at a height of 40-50 cm, while spraying the microbial fermentation agent for treating alcohol waste liquid according to claim 2 onto the filter sludge, mix well and bury it Cover it to allow it to ferment. After 2-3 days, mycelium appears and the temperature reaches 55 ° C-6 (TC). The pile is turned over every 5-7 days, and the fermentation is completed after 15 days.

(3) Comprehensive fermentation: The above steps (1) and (2) are performed simultaneously. After completion, the fermented alcohol solution obtained in step (1) and the fermented filter mud obtained in step (2) are pressed in a ratio of 1: 2 5- 3 (weight ratio) Mix, stir and ferment, the temperature is between 40 ° C-5 (TC), and it will become the active organic fertilizer raw material after turning over the heat and fermenting and drying.