CN101475973B - Bacteria cellulose production culture medium containing alcohol wastewater - Google Patents
Bacteria cellulose production culture medium containing alcohol wastewater Download PDFInfo
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- CN101475973B CN101475973B CN200910002472XA CN200910002472A CN101475973B CN 101475973 B CN101475973 B CN 101475973B CN 200910002472X A CN200910002472X A CN 200910002472XA CN 200910002472 A CN200910002472 A CN 200910002472A CN 101475973 B CN101475973 B CN 101475973B
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Abstract
The invention discloses a production culture medium of a bacteria cellulose including alcoholic waste liquor, which is characterized by mixing the natural substrates and alcohol waste liquor in proportion to obtain mixture, and then mixing uniformly the mixture, water, and sugar to obtain the bacteria cellulose production culture medium. The present invention can effectively reduce the required natural substrates proportionality in the bacteria cellulose production culture medium, reduce production costs and shorten the production cycle; but also finds a new way for the comprehensive utilization of the alcohol waste liquor, wherein, the produced bacteria cellulose products have higher added value and is suitable spread.
Description
Technical field
The present invention relates to a kind of microorganisms producing substratum, be specifically related to a kind of bacteria cellulose production culture medium of spirituosity waste liquid, utilize this substratum to produce bacteria cellulose, can shorten the production cycle, reduce production costs.
Background technology
Alcohol is a kind of important industrial raw material, is widely used and development prospect.Alcohol industry is again an industry that pollution is very serious but simultaneously.According to the literature, be raw material with cane molasses or cassava, the high concentrated organic wastewater of every production 1t alcohol discharging is about 14～15t, waste liquid COD
CrValue about 120000mg/L, BOD
5Value because its composition is quite complicated, makes the wastewater treatment difficulty bigger about 60000mg/L.The utilization of alcohol slops is insoluble for many years both at home and abroad problem with administering always, according in document " molasses alcohol waste liquid treatment technology " (" Treatment of Industrial Water " 2002 the 10th phases) literary composition, the method mainly taked of alcohol slops has at present: compost, aerobic treatment, anaerobic treatment, recycle, drying, burning etc.Though composting process energy qualified discharge, the amount of disposing waste liquid is limited; Though the bio-oxidation pond method can reduce the oxygen organic amount to a certain extent, but still can not reach emission standard, still pollutes the environment; Circulation needs by additive method, and up to standard not thorough; With alcohol slops evaporation concentration, drying, preparation fertilizer, feed are a kind of common methods of contaminated solution, but because evaporation concentration need consume lot of energy, the disposable input of burning is higher, and the big use of management difficulty producer is few.Carrying out environmental protection high-level today, alcohol slops is handled as the indispensable link of Alcohol Production, and the Alcohol Production cost is improved greatly.
Consider from economic angle, comprehensive utilization of waste materials is the major action that improves environment, increases economic efficiency, promotes national economy sustainable development, organism in the alcohol slops provides abundant basic substance for the resource utilization of various approach, particularly as the nutraceutical matrix of microorganism growth, the recycling of useful component in the alcohol slops is reached zero release, can guarantee again simultaneously treating processes with low cost should be following main direction.Certain research and discussion have also been done at present both at home and abroad in this respect, as having announced a kind of method of utilizing alcohol slops to improve cellulase activity among the Chinese patent CN 1149282C, it is to add alcohol waste liquid for sugar refinery in substratum, cellulase producing bacteria fermentation culture after sterilizing, the product that obtains can be made with extra care and be cellulase preparation.Announced a kind of treatment process of molasses-spirit molasses among the patent CN 1159235C, it be with sugarcane sugarcane marrow with after molasses alcohol waste liquid mixes by a certain percentage, get involved saprophytic edible fungus species cultivation, make compound become bacterium chaff material, obtain edible mushrooms simultaneously.Aforesaid method all is that the nutraceutical matrix of alcohol slops as microorganism culturing utilized again, but has certain defective, and causing producing can't large-scale promotion application.As: utilize alcohol slops to produce the purified zymin, still can't avoid pollution that the production process secondary emission brings and because the sky high cost that technologies such as concentrate drying are brought; Utilize alcohol slops to produce the edible mushrooms method, though do not have secondary emission and concentrate drying, but this value-added content of product is generally lower, be difficult to obtain gratifying economic benefit, and this method production cycle is longer, be difficult to form fairly large, this means and to utilize the huge quantity discharged of alcohol slops fully.
The Mierocrystalline cellulose that utilizes microorganism (mainly being acetobacter) fermentation to produce, generally be referred to as bacteria cellulose or micro organism cellulose, many constructional features that are different from plant cellulose such as this Mierocrystalline cellulose has the purity height, water-absorbent is strong, degree of crystallinity is high, polymerization degree height, its fiber bundle diameters is below 100 nanometers, it is a kind of natural nano meter biomaterial, be one of focus of biomaterial research and development in recent years, have a wide range of applications at the manufacturing and the paper industry of food, papermaking, acoustical vibration film, artificial skin.At present, this bacteria cellulose has aspect food more widely to be used, its main method is to be substratum with Sucus Cocois, pineapple water or other natural substrates, after the cellulosic acetobacter fermentation of multiparity, the edible bacterial cellulose gel film that produces is commonly called as " coconut palm fruit ", " high fibrous coconut ", " the fine fruit of coconut palm " or " Natta ".The development at full speed that the bacteria cellulose industry obtains in areas such as China Hainan Province, Vietnam, Philippines in recent years, its production capacity surpasses 500000t/a, because its demand is huge, product has the gesture that supply falls short of demand, present undressed bacteria cellulose selling price reaches about 1200 yuans per ton, the bacteria cellulose price of handling through deep processing reaches about 10000 yuans, and its added value of product is higher.The large-scale production of bacteria cellulose mainly is to utilize nutritious natural substrates such as Sucus Cocois, pineapple juice as substratum production at present; its ratio generally accounts for more than 30% of substratum gross weight; production cycle is generally more than 7 days; higher and the raw materials for production of this mode of production production cost are subjected to the restriction of season and region, are one of bottlenecks of bacteria cellulose industry development always.Also have bibliographical information to attempt reducing or producing bacteria cellulose, but production cycle and product quality are not fully up to expectations without these natural substrates.
Summary of the invention
The bacteria cellulose production culture medium that the purpose of this invention is to provide a kind of spirituosity waste liquid, it can reduce natural substrates ratio in the bacteria cellulose production culture medium effectively, can shorten the production cycle simultaneously.
The composition of alcohol slops is very complicated, the contriver is by analyzing, find in the alcohol slops except that containing higher protein and amino acid, also contain a certain amount of carbohydrate, organic acid, the residual body of yeast, inorganic salt and trace element simultaneously, these compositions can be used as the useful of microbiological culture media nutritive ingredient and replenish.The contriver is by discovering, because microbial metabolism is for the demand difference of nutritional factor, be not that all microorganisms all are suitable for growing in containing the substratum of alcohol slops, for some microorganisms, some composition of alcohol slops (as: certain density alcohol, acetate etc.) often becomes supressor, not only can not promote microbial growth, play restraining effect on the contrary; And for the other microorganism, some composition in the alcohol slops but becomes the inducible factor or the activator of fermentation target product, has promoted the generation of microbial growth breeding and target product in fermentation by synergy.
The contriver also finds, the ratio of alcohol slops in substratum also has remarkable influence to microbial growth, in the substratum of producing bacteria cellulose, add a spot of alcohol slops, insert gluconate pyracetobacillus (a kind of bacterium of energy high yield bacteria cellulose, strict aerobic, leave standstill the surface that the energy liquid medium within contacts with air when cultivating and produce bacteria cellulose film), do not have very obvious facilitation for the generation of cellulose membrane, but do not have obvious restraining effect yet; During ratio that alcohol slops furnishing in substratum is certain, but can play very obvious facilitation for the generation of cellulose membrane: the one, shorten the production cycle, the 2nd, the quality of resulting bacteria cellulose is better, and cellulosic content is higher.In addition,, can solve nitrogenous source and the insufficient problem of inorganic salt content in the natural fruit and vegetables juice substratum, replenish as the useful of nitrogenous source and inorganic salt, thereby reduced production cost owing to also contain a certain amount of protein and trace element in the alcohol slops.
The technical solution adopted in the present invention is:
A kind of bacteria cellulose production culture medium of spirituosity waste liquid, feature is to be that 4: 1～1: 9 ratio is mixed by weight with natural substrates and alcohol slops, obtain mixed solution, then mixed solution, water, sucrose are mixed, obtain bacteria cellulose production culture medium, wherein mixed solution accounts for 30%～70% of substratum gross weight, and sucrose accounts for 1%～5% of substratum gross weight, and surplus is a water.Described natural substrates is that Sucus Cocois, pineapple water or other are used for a kind of of natural nutrient solution that bacteria cellulose produces or mix.The interpolation of sucrose can be the culture medium supplemented carbon source.
Wherein preferred scheme is that the weight ratio of natural substrates and alcohol slops is 1: 1～1: 5 in mixed solution.
As all zymotechniques, the initial pH value of producing the bacteria cellulose substratum can produce significantly influence to fermentation.Produced bacteria cellulose generally is the medium pH value to be adjusted in about 4.0 just can obtain higher productive rate in the past, and because variation has taken place substratum, this empirical working condition can only be as a reference.By test, the pH value of substratum of the present invention is adjusted between 3.0～6.5 and can both obtains higher productive rate, and its preferred scheme is that the pH value is between 3.1～3.8 or between 5.1～6.3.
The bacteria cellulose production culture medium of spirituosity waste liquid provided by the present invention is by heat sterilization, and the bacterial classification that produces bacteria cellulose is inserted in the cooling back, and shallow tray fermentation can be gathered in the crops bacteria cellulose after 3～10 days.Adopt the aforesaid method shallow tray fermentation to produce bacteria cellulose, the production cycle can shorten, ferment after 4～5 days, and cellulose membrane thickness 〉=1.2cm, the production of Sucus Cocois substratum then generally needs 6～8 days.
Utilize the substratum of spirituosity waste liquid to produce bacteria cellulose, its remarkable advantages is:
1, can reduce required natural substrates ratio in the bacteria cellulose production culture medium, reduce production cost, can shorten the production cycle simultaneously.
2, find a kind of new approach of alcohol slops comprehensive utilization, turn waste into wealth, added value of product is higher, is fit to produce promote.
Embodiment
Below in conjunction with the indefiniteness specific embodiment, further set forth the present invention.Should be appreciated that these embodiment only to be used to the present invention is described and be not used in restriction the scope of protection of present invention.
Embodiment one
Sucus Cocois 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute, wherein add the sucrose supplementary carbon source of 200g again, so just constituted the new substratum of a kind of spirituosity waste liquid at adding 5kg water in mixed solution.Substratum is regulated pH value 3.8, and then by heat sterilization, gluconate pyracetobacillus is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.2cm.
Embodiment two
Sucus Cocois 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute at adding 5kg water in mixed solution, the sucrose that wherein adds 200g has so just constituted the new substratum of a kind of spirituosity waste liquid as carbon source again.Substratum is regulated pH value 3.8, and then by heat sterilization, acetobacter xylinum is inserted in the cooling back, and shallow tray fermentation is after 4 days, and the bacteria cellulose film thickness reaches 1.2cm.
Embodiment three
Sucus Cocois 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute at adding 5kg water in mixed solution, the sucrose that wherein adds 300g has so just constituted the new substratum of a kind of spirituosity waste liquid as carbon source again.Substratum is regulated pH value 3.8 then by heat sterilization, and acetobacter xylinum is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.5cm.
Embodiment four
Sucus Cocois 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute at adding 5kg water in mixed solution, the sucrose that wherein adds 300g has so just constituted the new substratum of a kind of spirituosity waste liquid as carbon source again.Substratum is regulated pH value 6.0, and then by heat sterilization, gluconate pyracetobacillus is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.3cm.
Embodiment five
Sucus Cocois 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute in mixed solution, adding 5kg water, wherein add again account for 300g sucrose as carbon source, so just constituted the new substratum of a kind of spirituosity waste liquid.Substratum is regulated pH value 6.0 then by heat sterilization, and acetobacter xylinum is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.5cm.
Embodiment six
Pineapple water 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute at adding 5kg water in mixed solution, the sucrose that wherein adds 300g has so just constituted the new substratum of a kind of spirituosity waste liquid as carbon source again.Substratum is regulated pH value 3.8 then by heat sterilization, and acetobacter xylinum is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.5cm.
Embodiment seven
Pineapple water 1kg and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute at adding 5kg water in mixed solution, the sucrose that wherein adds 300g has so just constituted the new substratum of a kind of spirituosity waste liquid as carbon source again.Substratum is regulated pH value 6.0, and then by heat sterilization, gluconate pyracetobacillus is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.3cm.
Embodiment eight
Sucus Cocois, each 0.5kg of pineapple water and alcohol slops 4kg are mixed, obtain the 5kg mixed solution, dilute in mixed solution, adding 5kg water, wherein add again account for 300g sucrose as carbon source, so just constituted the new substratum of a kind of spirituosity waste liquid.Substratum is regulated pH value 6.0 then by heat sterilization, and acetobacter xylinum is inserted in the cooling back, and shallow tray fermentation is after 5 days, and the bacteria cellulose film thickness reaches 1.5cm.
Claims (3)
1. the bacteria cellulose production culture medium of a spirituosity waste liquid, it is characterized in that: with natural substrates and alcohol slops is that 4: 1～1: 9 ratio is mixed by weight, obtain mixed solution, then mixed solution, water, sucrose are mixed, obtain bacteria cellulose production culture medium, the pH value of substratum is adjusted between 3.0～6.5, and wherein mixed solution accounts for 30%～70% of substratum gross weight, sucrose accounts for 1%～5% of substratum gross weight, and surplus is a water; Described natural substrates is a kind of or mixing in Sucus Cocois or the pineapple water.
2. the bacteria cellulose production culture medium of spirituosity waste liquid according to claim 1 is characterized in that: the weight ratio of natural substrates and alcohol slops is 1: 1～1: 5 in mixed solution.
3. the bacteria cellulose production culture medium of spirituosity waste liquid according to claim 1 is characterized in that: between the pH value of described substratum is adjusted in 3.1～3.8 or between 5.1～6.3.
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| CN102250985B (en) * | 2011-08-05 | 2017-07-11 | 海南椰国食品有限公司 | Fermentation medium for bacterial cellulose |
| ES2888002B2 (en) | 2020-06-01 | 2022-05-26 | Consejo Superior Investigacion | PRODUCTION OF BACTERIAL NANOCELLULOSE |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377958A (en) * | 2002-04-30 | 2002-11-06 | 高明金葵子植物营养有限公司 | Microbial fermentation agent for treating waste alkohol liquid and its preparing method |
| CN1807592A (en) * | 2006-01-04 | 2006-07-26 | 大连春天生物技术有限公司 | Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377958A (en) * | 2002-04-30 | 2002-11-06 | 高明金葵子植物营养有限公司 | Microbial fermentation agent for treating waste alkohol liquid and its preparing method |
| CN1807592A (en) * | 2006-01-04 | 2006-07-26 | 大连春天生物技术有限公司 | Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid |
Non-Patent Citations (2)
| Title |
|---|
| 王志国,钟春燕.椰子水自然发酵条件对细菌纤维素生产的影响.中国酿造205 4.2009,205(4),32-34. * |
| 邓毛程.木醋杆菌利用酵母发酵滤液产高纤椰果的研究.中国酿造186 9.2008,186(9),71-73. * |
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