CN1693450A - Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria - Google Patents
Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria Download PDFInfo
- Publication number
- CN1693450A CN1693450A CNA2005100497049A CN200510049704A CN1693450A CN 1693450 A CN1693450 A CN 1693450A CN A2005100497049 A CNA2005100497049 A CN A2005100497049A CN 200510049704 A CN200510049704 A CN 200510049704A CN 1693450 A CN1693450 A CN 1693450A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- milliliters
- substratum
- yellow
- function yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002361 compost Substances 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 239000001963 growth medium Substances 0.000 title claims description 26
- 241000894006 Bacteria Species 0.000 title abstract description 3
- 229930006000 Sucrose Natural products 0.000 claims abstract description 19
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 30
- 238000000855 fermentation Methods 0.000 claims description 26
- 230000004151 fermentation Effects 0.000 claims description 26
- 239000002351 wastewater Substances 0.000 claims description 23
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 239000005720 sucrose Substances 0.000 claims description 18
- 229940049906 glutamate Drugs 0.000 claims description 17
- 229930195712 glutamate Natural products 0.000 claims description 17
- 238000011177 media preparation Methods 0.000 claims description 12
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- 238000007599 discharging Methods 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 241000499912 Trichoderma reesei Species 0.000 claims description 5
- 230000000050 nutritive effect Effects 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 229960002989 glutamic acid Drugs 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 235000013922 glutamic acid Nutrition 0.000 abstract 1
- 239000004220 glutamic acid Substances 0.000 abstract 1
- 239000010865 sewage Substances 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 210000003608 fece Anatomy 0.000 description 12
- 244000144972 livestock Species 0.000 description 12
- 239000010871 livestock manure Substances 0.000 description 12
- 244000144977 poultry Species 0.000 description 12
- 239000003337 fertilizer Substances 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 8
- 239000002699 waste material Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 239000002068 microbial inoculum Substances 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 244000063299 Bacillus subtilis Species 0.000 description 5
- 241000726221 Gemma Species 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000009264 composting Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000003895 organic fertilizer Substances 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 2
- 239000004223 monosodium glutamate Substances 0.000 description 2
- 235000013923 monosodium glutamate Nutrition 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000406668 Loxodonta cyclotis Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BTZFRMSCJNAZGT-OYIAIMIOSA-L [Na+].[Na+].N[C@@H](CCC(O)=O)C([O-])=O.N[C@@H](CCC(O)=O)C([O-])=O Chemical compound [Na+].[Na+].N[C@@H](CCC(O)=O)C([O-])=O.N[C@@H](CCC(O)=O)C([O-])=O BTZFRMSCJNAZGT-OYIAIMIOSA-L 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 229920006237 degradable polymer Polymers 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
A process for preparing the culture meidum of arerobic high-temp fermenting bacteria to prepare compost includes such steps as collecting the organic sewage generated by food industry, the slops generated in production of bean products and the tailing liquid in producing glutamic acid, and mixing them with water and less cane sugar (or not).
Description
Technical field
The invention belongs to biological technical field, relate to a kind of aerobic high-heat compost fermentation function yeast culture medium preparation, the high concentrated organic wastewater that particularly utilizes foodstuffs industry to discharge is prepared the method for aerobic During High-Temperature Composting fermentation function yeast substratum.
Background technology
Along with the high speed development of foodstuffs industry, the useless poor high concentrated organic wastewater that reaches of the waste residue of generation has caused huge pressure to environmental protection.Industry is produced as monosodium glutamate monosodium glutamate waste liquor rich in proteins, amino acid, thalline and NH
4 +, K
+, Na
+, Mg
2+, Ca
2+, Fe
2+, Cl
-, SO
4 2-, PO
4 3-Deng mineral ion, and for example the bean product waste liquid contains 17 seed amino acids, 1.6% solid substance, its COD
CrReach more than 20000～25000mg/L, all belong to high concentrated organic wastewater, if directly discharging can cause serious environmental to pollute, if by the pollution treatment discharging, its working cost brings white elephant to enterprise.
On the other hand, the pollution that brings of livestock and poultry cultivation development is serious day by day.In its main control measures, making the best buy fertilizer by the aerobic high-heat fermentation maturity is to be worth the effective way advocated energetically: (1) owing to carry out the batch production large-scale production, so the amount of dissolving of manure of livestock and poultry is big, and the processing efficiency height; (2) manure of livestock and poultry is made the high-quality fertilizer, makes wherein energy and nutrient obtain recycle, resource utilization degree height; (3) organic fertilizer product made of manure of livestock and poultry thermophilic fermentation, not only contain N, P, K and trace element and directly provide these nutritive substances for crop, and be good soil redeposition, the a considerable number of soil ulmin that it is contained, it is complicated degradable polymer organic colloidal matter, humic acid wherein etc. can directly promote plant-growth, can also improve the exchange capacity and the moisture retention of soil after being manured into soil, adsorb necessary for plant growth nitrogen, phosphorus, potassium and trace element effectively, keep the lasting fertility of soil; Contain a large amount of bacteriums, actinomycetes and fungi in the organic fertilizer product, not only increase these microbial numbers in the soil, new microbial population also is provided.Simultaneously, this fertilizer still is a kind of novel slowly-releasing source of manure.It is reported that organic commercial fertilizer nitrogen leaching in soilless substrate discharges steadily slow than chemical fertilizer.Therefore this organic fertilizer product meets non-polluted farm product and the special-purpose fertile requirement of green agricultural product production.Obviously, utilize manure of livestock and poultry to produce the special-purpose fertilizer of green agricultural product and be not only the effective way of administering the manure of livestock and poultry pollution, and will provide important material guarantee, realize changing rejected material to useful resource, the benign cycle of protection Agro-ecology for " making green agriculture ".
Microorganism in the manure of livestock and poultry aerobic composting process mainly has two sources, and the one, a large amount of microbial populations of inherent in the manure of livestock and poultry; The 2nd, be fermenting agent from the function bacterial classification of artificial adding.The different entrained microbial population type difference in the source of manure of livestock and poultry are bigger, and iff utilizing indigenous flora to carry out compost fermentation, often compost heats up slowly, and the composting time is long, the organic fertilizer production cycle stretch-out, and manufacturing enterprise's benefit is low.According to the Microbiological Principle in the composting fermentation process, separate, screen the bacterial classification of difference in functionality, making fermentation function microbial inoculum through artificial compound cultivation inoculates manure of livestock and poultry, can not only quicken the composting process, shorten the compost time, can also control malodorous generation, reduce nitrogen loss, improve the quality of finished product fertilizer.
At present, manure of livestock and poultry fermenting agent commercialization has in the world been squeezed into China market as EM, the enzymatic microorganism of Japan, and domestic also have the analogous products listing, but the microbial inoculum selling at exorbitant prices, the enzymatic microorganism price per ton of Japan is up to 200,000 yuan.According to estimates, the microbial inoculum cost is up to 20～60% of the fertilizer total cost.The microbial inoculum cost of great number makes the performance of enterprises low even profitless, and enterprise is difficult to sustainable development.Obviously, developing the function microbial inoculum that efficiently ferments of the manure of livestock and poultry with independent intellectual property right, reduce the production cost of fermentation function yeast, make enterprise have microbial inoculum throughput, is the important channel of breaking through restriction fertilizer enterprise development bottleneck.
The main raw material of its substratum of existing fermentation function yeast such as subtilis (Bacillus subtilis) is: sucrose, starch, dregs of beans, manganous sulfate, dipotassium hydrogen phosphate, potassium primary phosphate etc.A kind of bacillus subtilis bacterium culture medium of optimization is: starch 0.3%, sucrose 0.5%, the MnSO of 30.8mg/kg
40.2%,, KzHPO
40.3%, KHzPO
40.15%, dregs of beans 3%, PH7.0-7.5 (" fodder industry " 2004 the 25th the 7th phases of volume, the optimization of fermentation of bacillus subtilis process).
The objective of the invention is to: the high concentrated organic wastewater of foodstuffs industry discharging effectively is used in the cultivation of aerobic high-heat compost fermentation function yeast, reduces the production cost of fermentation function yeast, and the high concentrated organic wastewater of dissolving.
Summary of the invention
The aerobic high-heat compost fermentation function yeast culture medium preparation method that the present invention proposes is: adopt the yellow swill of discharging in the bean product production, or remaining glutamate wastewater after the L-glutamic acid production, or the mixed solution of yellow swill and glutamate wastewater is mixed into three kinds of liquid nutrient mediums with water as the nutritive ingredient in the substratum respectively with above-mentioned three kinds of nutritive ingredients.
The fermentation function yeast that is used to cultivate by the substratum of present method preparation comprises: subtilis, Bacillus licheniformis, soak numb genus bacillus, Trichodermareesei and series bacillus.
Can in three kinds of liquid nutrient mediums, add sucrose 5～30 grams respectively.
As preferred subtilis culture medium prescription be: contain 300 milliliters of yellow swills in every liter of substratum, sucrose 20 grams, surplus is a water.
As preferred Bacillus licheniformis culture medium prescription be: contain 300 milliliters of yellow swills in every liter of substratum, 50 milliliters of glutamate wastewaters, sucrose 10 grams, surplus is a water.
As preferably soaking numb bacillus culture medium prescription be: contain 200 milliliters of yellow swills in every liter of substratum, 25 milliliters of glutamate wastewaters, sucrose 5 grams, surplus is a water.
As preferred Trichodermareesei culture medium prescription be: contain 300 milliliters of yellow swills in every liter of substratum, sucrose 10 grams, surplus is a water.
As preferred series bacillus culture medium prescription be: contain 200 milliliters of yellow swills in every liter of substratum, 100 milliliters of glutamate wastewaters, surplus is a water.
Embodiment
In order to specify embodiments of the present invention and to prove its result of use, collected the substratum of West Lake Gourmet Powder Factory monosodium glutamate waste liquid (glutamate wastewater), Hangzhou bean product Food Co., Ltd yellow swill preparation fermentation function yeast, after measured, the essential nutrient contents of two kinds of waste liquids is as shown in table 1.
N, the P of two kinds of organic liquid wastes of table 1, K nutrient content
| Nutrient | Monosodium glutamate waste liquid | Yellow swill |
| Full N (g/L) | ????12.73 | ????0.70 |
| Full P (mg/L) | ????167.18 | ????98.93 |
| Full K (g/L) | ????20.7 | ????0.75 |
Embodiment 1: bacillus subtilis bacterium culture medium compound method:
Get 300 milliliters of yellow swills, do not add glutamate wastewater, add sucrose 20 grams, add water to 1000 milliliters of constant volumes.
Used preceding 121 ℃ of vapor sterilizations 20 minutes, insert Bacillus subtilis strain after being cooled to 30 ℃, putting into vibration shaking table incubator cultivates for 20～30 ℃ and regulates ventilation than being 1: 1, make the relative value of oxygen dissolving value be not less than 20, keep pH to be not less than 6.5, shaking culture played sampling in per 4 hours in 20 hours and detects the gemma rate of formation once, and cultivating and detecting the gemma rate of formation in 32 hours is 97%, finish to cultivate, measuring viable count with the plate live bacterial count is 2.4 * 10
13/ milliliter.
Embodiment 2: Bacillus licheniformis substratum compound method:
Contain 300 milliliters of yellow swills in per 1000 milliliters of substratum, 50 milliliters of glutamate wastewaters, sucrose 10 grams, surplus is a water.
Cultural method and process are with embodiment 1.Cultivate end in 24 hours, detecting the gemma rate of formation is 97.4%, and measuring viable count with the plate live bacterial count is 1.7 * 10
13/ milliliter.
Embodiment 3: soak numb bacillus culture medium compound method:
Contain 200 milliliters of yellow swills in per 1000 milliliters of substratum, 25 milliliters of glutamate wastewaters, sucrose 5 grams, surplus is a water.
Cultural method and process are with embodiment 1.Cultivate end in 24 hours, detecting the gemma rate of formation is 97.6%, and measuring viable count with the plate live bacterial count is 2.5 * 10
13/ milliliter.
Embodiment 4: Trichodermareesei substratum compound method:
Contain 300 milliliters of yellow swills in per 1000 milliliters of substratum, do not add glutamate wastewater, add sucrose 10 grams, surplus is a water.
Cultural method and process are with embodiment 1.Cultivate end in 48 hours, measuring viable count with the plate live bacterial count is 2.7 * 10
12/ milliliter.
Embodiment 5: series bacillus substratum compound method:
Contain 200 milliliters of yellow swills in per 1000 milliliters of substratum, 100 milliliters of glutamate wastewaters, not with sucrose, surplus is a water.
Cultural method and process are with embodiment 1.Cultivate end in 48 hours, detecting the gemma rate of formation is 97.7%, and measuring viable count with the plate live bacterial count is 2.7 * 10
13/ milliliter.
A kind of aerobic high-heat fermentation function yeast culture medium preparation method provided by the invention, yellow swill and two kinds of high concentrated organic wastewaters of glutamate wastewater of foodstuffs industry discharging have been utilized, add or do not add a spot of sucrose, need not to use other compositions such as starch, dregs of beans, effectively reduce production cost, rationally utilize waste resource, alleviate environmental protection pressure, and provide the function bacterial classification that ferments cheaply for the improvement of holding fowl breed pollution again.
Claims (8)
1, a kind of aerobic high-heat compost fermentation function yeast culture medium preparation method, it is characterized in that: adopt the yellow swill of discharging in the bean product production, or remaining glutamate wastewater after the L-glutamic acid production, or the mixed solution of yellow swill and glutamate wastewater is mixed into three kinds of liquid nutrient mediums with water as the nutritive ingredient in the substratum respectively with above-mentioned three kinds of nutritive ingredients.
2, by the described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation method of claim 1, it is characterized in that: the fermentation function yeast that the substratum of described method preparation is used to cultivate comprises: subtilis, Bacillus licheniformis, soak numb genus bacillus, Trichodermareesei and series bacillus.
3, by the described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation method of claim 1, it is characterized in that: in three kinds of liquid nutrient mediums, add sucrose 5～30 grams respectively.
4, by claim 1 or 3 described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation methods, it is characterized in that: be as preferred subtilis culture medium prescription: contain 300 milliliters of yellow swills in every liter of substratum, sucrose 20 grams, surplus is a water.
5, by claim 1 or 3 described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation methods, it is characterized in that: be: contain 300 milliliters of yellow swills in every liter of substratum as preferred Bacillus licheniformis culture medium prescription, 50 milliliters of glutamate wastewaters, sucrose 10 grams, surplus is a water.
6, by claim 1 or 3 described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation methods, it is characterized in that: as preferably soaking numb bacillus culture medium prescription be: contain 200 milliliters of yellow swills in every liter of substratum, 25 milliliters of glutamate wastewaters, sucrose 5 grams, surplus is a water.
7, by claim 1 or 3 described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation methods, it is characterized in that: be as preferred Trichodermareesei culture medium prescription: contain 300 milliliters of yellow swills in every liter of substratum, sucrose 10 grams, surplus is a water.
8, by claim 1 or 3 described a kind of aerobic high-heat compost fermentation function yeast culture medium preparation methods, it is characterized in that: be: contain 200 milliliters of yellow swills in every liter of substratum as preferred series bacillus culture medium prescription, 100 milliliters of glutamate wastewaters, surplus are water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100497049A CN100362093C (en) | 2005-04-29 | 2005-04-29 | Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100497049A CN100362093C (en) | 2005-04-29 | 2005-04-29 | Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1693450A true CN1693450A (en) | 2005-11-09 |
| CN100362093C CN100362093C (en) | 2008-01-16 |
Family
ID=35352577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100497049A Expired - Fee Related CN100362093C (en) | 2005-04-29 | 2005-04-29 | Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100362093C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102191188A (en) * | 2010-03-10 | 2011-09-21 | 中国科学院福建物质结构研究所 | Method for culturing bacteria and application thereof |
| CN102786934A (en) * | 2012-08-22 | 2012-11-21 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN101809151B (en) * | 2007-08-30 | 2013-04-24 | 埃欧金能源公司 | Method for cellulase production |
| CN105331553A (en) * | 2015-10-20 | 2016-02-17 | 西南科技大学 | Preparation method of complex microbial inoculant for high-temperature rapid composting of breeding and slaughtering solid wastes |
| CN109136124A (en) * | 2018-08-17 | 2019-01-04 | 西南科技大学 | A kind of quick volume reduction decrement treatment composite bacteria agent of super enriching plant body and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1187947A (en) * | 1997-06-10 | 1998-07-22 | 李红光 | Production of yeast fodder by concentrated treatment of ethyl alcohol or aginomoto waste water |
-
2005
- 2005-04-29 CN CNB2005100497049A patent/CN100362093C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101809151B (en) * | 2007-08-30 | 2013-04-24 | 埃欧金能源公司 | Method for cellulase production |
| CN102191188A (en) * | 2010-03-10 | 2011-09-21 | 中国科学院福建物质结构研究所 | Method for culturing bacteria and application thereof |
| CN102786934A (en) * | 2012-08-22 | 2012-11-21 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN102786934B (en) * | 2012-08-22 | 2015-04-22 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN105331553A (en) * | 2015-10-20 | 2016-02-17 | 西南科技大学 | Preparation method of complex microbial inoculant for high-temperature rapid composting of breeding and slaughtering solid wastes |
| CN109136124A (en) * | 2018-08-17 | 2019-01-04 | 西南科技大学 | A kind of quick volume reduction decrement treatment composite bacteria agent of super enriching plant body and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100362093C (en) | 2008-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101209933B (en) | Refined biological organic fertilizer and its preparing process | |
| CN104003804B (en) | A kind of composite microbiological fertilizer of holding water and fixing nitrogen and preparation method thereof | |
| CN102876608B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN111635284B (en) | Preparation method of polyglutamic acid charcoal-based organic fertilizer | |
| CN1781881A (en) | Method for producing useful organic substance using industrial and agricultural organic wastes | |
| CN110241041B (en) | Compound microbial preparation, preparation method and application thereof | |
| CN111073839B (en) | Siam bacillus, microbial inoculum and application thereof | |
| CN100569704C (en) | A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate | |
| CN104744182A (en) | Tobacco water retention biological organic fertilizer and preparation method thereof | |
| CN110330370A (en) | While a kind of turf biological organic fertilizer and its preparation method and application containing trichoderma and bacillus amyloliquefaciens | |
| CN1254696A (en) | Compound biological fertilizer | |
| CN108264405A (en) | A kind of organic fertilizer prepared using cassava trade waste and preparation method thereof | |
| CN100362093C (en) | Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria | |
| CN105505843A (en) | Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure | |
| CN1482105A (en) | Method of producing organic fertilizer by fermenting saponin fag-end and wastewater | |
| CN102219575B (en) | Industrial method for producing bio-fertilizer inoculant by utilizing decayed cow dung as raw material | |
| CN101955903A (en) | Bacillus licheniformis bacterial strain and application thereof | |
| CN1062253C (en) | Solid-nitrogen bud-fungus fertilizer and production method thereof | |
| CN1467180A (en) | Method for producing biotechnical fulvic acid composite biological bacteria fertilizer by microorganism fermentation of sugar grass straw | |
| CN115386508B (en) | Bacillus licheniformis and application thereof | |
| CN106609250A (en) | Preparation method of fermented traditional Chinese medicine residue organic fertilizer inoculant and application thereof | |
| CN106520740B (en) | Preparation method and application of biological fermentation high-efficiency amino acid type organic enzyme preparation | |
| CN102399711A (en) | Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof | |
| CN101475973B (en) | Bacteria cellulose production culture medium containing alcohol wastewater | |
| CN101456761B (en) | Method for preparing microbial organic fertilizer for controlling crop blight |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Jinhua Zhejiang Fengyuan agricultural science and Technology Co., Ltd. Assignor: Zhejiang University Contract record no.: 2011330000838 Denomination of invention: Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria Granted publication date: 20080116 License type: Exclusive License Open date: 20051109 Record date: 20110628 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080116 Termination date: 20160429 |