WO2003093454A1 - Preparation microbienne traitant les residus alcooliques, son procede d'obtention, et procede de fermentation associe - Google Patents

Preparation microbienne traitant les residus alcooliques, son procede d'obtention, et procede de fermentation associe Download PDF

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Publication number
WO2003093454A1
WO2003093454A1 PCT/CN2003/000224 CN0300224W WO03093454A1 WO 2003093454 A1 WO2003093454 A1 WO 2003093454A1 CN 0300224 W CN0300224 W CN 0300224W WO 03093454 A1 WO03093454 A1 WO 03093454A1
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WO
WIPO (PCT)
Prior art keywords
strain
fermentation
waste liquid
flora
microbial
Prior art date
Application number
PCT/CN2003/000224
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English (en)
Chinese (zh)
Inventor
Biu Ho
Enhsiung Huang
Original Assignee
Gaoming Jinkuizi Plant Nutriment Co.,Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gaoming Jinkuizi Plant Nutriment Co.,Ltd. filed Critical Gaoming Jinkuizi Plant Nutriment Co.,Ltd.
Priority to AU2003227171A priority Critical patent/AU2003227171A1/en
Publication of WO2003093454A1 publication Critical patent/WO2003093454A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Definitions

  • the invention relates to the technical field of alcohol waste liquid treatment, in particular to a microorganism fermentation agent for treating alcohol waste liquid, a preparation method thereof and a fermentation process. Background technique
  • the current methods for treating alcohol waste are:
  • Direct discharge method This method is suitable for non-sandy farmland with large areas outside the plant that can withstand wastewater purification and lack water. However, waste liquid such as seepage into rivers or groundwater sources can cause pollution.
  • Alcohol waste liquid concentration method After the multi-effect evaporation system is used to concentrate the alcohol waste liquid, it is processed into a powder by high-temperature spray treatment before it can be used. The equipment investment is large and other costs are also large. The powder after treatment is easy to absorb moisture and agglomerate.
  • An object of the present invention is to provide a microbial flora capable of treating alcohol waste liquid.
  • Another object of the present invention is to provide a microbial fermentation agent for treating alcohol waste liquid, which can quickly and efficiently ferment alcohol waste liquid into a raw material of active organic fertilizer.
  • Another object of the present invention is to provide a method for preparing the fermentation agent.
  • the microbial flora for treating alcohol waste liquor provided by the present invention comprises: Bacillus polymyxa CGMCC 0395.1, named 1 species ; Bacillus subtilis CGMCC 0395.2, named species 2; Bacillus brevis CGMCC 0395.3, named species 3; Bacillus licheniformis CGMCC 0395.4, named species WH4; Brevibacterium thiochroman CGMCC 0395.5, named species 5 Bacteria.
  • the main components of the microbial fermentation agent for treating alcohol waste liquid provided by the present invention include a microbial flora and a culture medium composed of the above five kinds of bacteria.
  • the culture medium preferably has the following formula: (weight percentage) rice bran: 70-80, soybean meal: 10 -20, milk meal: 2-5, meat and bone meal: 0.1- 0.2, fish bone meal: 0.1- 0.2, sugar: 1- 3. Water: 1-2.
  • the microbial fermentation agent is further preferred, and the proportion of the strains contained in the microbial flora is (weight percentage): Li 1 strain: 20-35, Li 2 strain: 5-20, TO3 strain: 15 — 30, Li 4 strains: 8—15, WH5 strains: 22—30.
  • the method for preparing the microbial fermentation agent provided by the present invention includes the following steps:
  • Secondary strain culture Firstly mix the secondary strain culture medium according to the above formula and mix the hooks, inoculate the book flora at normal temperature, pile up and cover, the temperature starts to rise, and the temperature reaches 35 ° C-40 after 2 days. ° C, after opening the cover for 30 minutes for natural heat dissipation every day, cover it for 8-10 days, and obtain the secondary active furfur bacteria of WH flora, which will become a microbial fermentation agent for treating alcohol waste liquid.
  • the proportion of the bacteria contained in the microbial flora in step (1) is (weight percentage): Book 1 bacteria: 20—35, 2 bacteria: 5-20, WH3 bacteria: 15—30 , Book 4 strains: 8-15, WH5 strains: 22-30.
  • the method for treating alcohol waste liquid by the microbial fermentation agent provided by the present invention includes the following steps:
  • Tertiary strain culture use alcohol waste liquid as tertiary strain culture medium
  • Fermentation process of album bacteria Add the microbial fermentation agent to the alcohol waste liquid, stir evenly, and inflate intermittently, three times a day, 15-30 minutes each time, the temperature is 24 ° C-38 ° C, PH value 3.5 -7.5, after 10-14 days, at least the surface foam of the liquid will increase, the liquid will change from thick to thin, the color will change from dark brown to brown, and the hammer will decrease from 50-60 degrees at the beginning to 43-46 degrees, that is, the fermentation is complete. ;
  • Filter sludge fermentation transport fresh filter sludge to the open space of the drying field and spread it at a height of 40-50 cm. At the same time, spray the microbial fermentation agent for treating alcohol waste liquid onto the filter sludge, mix it with a hook, and bury it to cover. To allow it to ferment, hyphae will appear after 2 to 3 days, and the temperature will reach 55 ° C-60 ° C. The piles will be turned every 5-7 days, and the fermentation will be completed after 15 days.
  • Steps (1) and (2) of step J are performed simultaneously. After completion, the fermented alcohol waste liquid obtained in step (1) and the fermented filter mud obtained in step (2) are pressed as follows: 2.5—3 (weight ratio) Mixed, stirred and fermented, the temperature is between 4 (rC_5 (TC), and it will become the active organic fertilizer raw material after turning over the heat and fermenting and drying. Compared with the prior art, the present invention has significant social benefits. Ecological and economic benefits:
  • the use of the microbial fermentation agent of the present invention can completely solve the three waste problems of sugar factories and alcohol factories, which is beneficial to production, environmental protection and the relationship between factories and groups.
  • the microbial flora of the microbial fermentation agent for the treatment of alcoholic waste liquid is composed of Bacillus polymyxa CGMCC 0395.1, named TO1 strain; Bacillus subtilis CGMCC 0395.2, named WH2 strain; Bacillus brevis CGMCC0395. 3, named the 3 species of bacteria; Bacillus licheniformis CGMCC 0395. 4, named WH4 strains; and Brevibacterium thiochroman CGMCC 0395. 5, named Li 5 strains to form the Li flora.
  • the proportion of the above microbiome is (weight percent):
  • the medium for secondary bacteria culture is prepared according to the following formula (weight percentage): rice bran: 80 soy flour: 16 milk powder: 2 meat and bone meal: 0.1 fish bone meal: 0.1 sugar: 0.8 water: 1 ;
  • the prepared secondary bacteria culture medium was inoculated with the above-mentioned TO flora at normal temperature, and white hyphae appeared on the medium the next day.
  • the culture temperature rose to 35 ° C, the pH value was 7.0, naturally radiated, and turned once after hardening.
  • the secondary active mycoplasma of WH flora was obtained within 10 days, which is the alcohol fermentation broth.
  • a method for treating alcohol waste liquid with a microbial fermentation agent comprising the following steps:
  • Filter mud fermentation transport fresh filter mud to the open space of the drying field and spread it out.
  • the height is 40cm, and the length and width are determined by the size of the drying field. Cover it with thin tape to allow it to ferment naturally.
  • Mycelia appear white, yellow, and green at 2 days after inoculation, and the temperature can reach 55 ° C. The piles of maggots are turned over every 5 days, and the fermentation is completed in 15 days.
  • the microbial flora of the microbial fermentation agent for treating alcohol waste liquid is the same as in Example 1, but the ratio of the WH strains of the microbial flora is (weight percentage):
  • the medium for secondary bacteria culture is prepared according to the following formula (weight percentage): rice bran: 70 soy flour: 20 milk powder: 5 meat and bone meal: 0.2
  • the prepared secondary bacteria culture medium was inoculated with the above-mentioned bacterial flora at normal temperature. Two days later, white hyphae appeared in the culture medium, and the culture temperature rose to 38 ° C. 9, naturally dissipates heat, flips once after hardening, and cultivates for 9 days to obtain secondary active mycobacterium of WH flora.
  • a method for treating alcohol waste liquid with a microbial fermentation agent including the following steps:
  • Fermentation of filter mud transport fresh filter mud to the open space of the drying ground and spread it out.
  • the height is 45cm, and the length and width are determined by the size of the drying ground. Cover it with a thin tape to allow it to ferment naturally.
  • Mycelia appear white, yellow, and green at 2 days after inoculation. The temperature can reach 57 ° C. The piles of pupae are turned over every 6 days, and the fermentation is completed in 15 days.
  • the microbial flora of the microbial fermentation agent for treating alcohol waste liquid is the same as in Example 1, but the ratio of the WH strains of the microbial flora is (weight percentage):
  • the culture medium of the secondary bacteria culture is prepared according to the following formula (weight percentage): rice bran: 75 soy flour: 17 milk powder: 3.1 meat and bone meal: 0.2 fish bone meal: 0.2 sugar: 2.5 Water: 2;
  • the prepared secondary bacteria culture medium was inoculated with WH flora at normal temperature. Two days later, white hyphae appeared in the culture medium, the culture temperature rose to 40 ° C, PH value was 6. 8, and the heat was released naturally. The secondary active mycoplasma of WH flora was obtained for 8 days.
  • a method for treating alcohol waste liquid with a microbial fermentation agent including the following steps:
  • the alcohol liquor is transferred to the fermentation tank.
  • the gas is intermittently pumped three times a day for 30 minutes at a temperature of 24 ° C and a pH value of 7. 5, after 14 days, the surface of the fermentation liquid
  • the foam changes from at least, the liquid changes from thick to thin, the color changes from dark to brown, and the hammer decreases from 60 degrees at the beginning to 46 degrees, that is, the fermentation is complete.
  • Filter mud fermentation transport fresh filter mud to the open space of the drying field and spread it out. The height is 45cm. The length and width are determined by the size of the drying field. Cover it with a thin plastic sheet to allow it to ferment naturally. Hyphae appear white, yellow, and green after 3 days of inoculation, and the temperature can reach 60 ° C. The pile of pupae is turned over every 7 days, and the fermentation is completed in 15 days.
  • Concentrated alcohol waste liquid is transported from the storage tank to the fermentation tank, and the bacteria flora alcohol waste liquid fermentation agent is added, and the air is intermittently pumped for 15-30 minutes three times a day.
  • the microorganisms multiply and ferment in the tank at a temperature of 24-38 ° C. , PH value 6. 3-6. 6, After 12 days, the surface of the liquid foamed at least, the liquid changed from thick to thin, the color changed from dark to brown, and the hammer decreased from 60 degrees at the beginning to 46 degrees, that is, fermentation carry out.
  • the fermented mature alcohol waste liquid is sprayed on the fermented filter sludge 2 to 3 times for mixing, stirring, secondary fermentation, and drying.
  • the mixing ratio of the filter sludge and the alcohol concentrated waste liquid is 2.5 to 3 tons.
  • the filter sludge absorbs 1 ton of alcohol waste liquid, and the bagasse ash is added, and the pile is turned according to the fermentation situation, and the fermentation is dried.
  • the fermentation temperature is maintained at 40-50. ° C, after drying, it is the raw material of active organic fertilizer.
  • Guangxi Fenghuang Sugar Factory has expanded the daily production of sugarcane due to the expansion of the production scale of the factory in recent years.
  • the medium filtration sludge reaches 25 thousand tons, sugar cane ash 5,000 tons, and alcohol waste liquid 12 thousand tons.
  • the discharge of these malodorous waste residue waste liquid seriously affects the living environment of the surrounding residents and also constitutes the location of the Phoenix River.
  • One of the sources of pollution, so cleaning up these wastes and ensuring the cleanliness of the environment has become an urgent problem.
  • the alcohol waste liquid biological fermentation agent of the present invention is used to comprehensively treat the above waste slag waste liquid to turn waste into treasure and turn harm into profit, and produce raw materials capable of producing high-quality active organic fertilizer.
  • This method for comprehensively treating waste residue and waste liquid is easy to implement, requires less investment, and has high benefits. It not only solves environmental protection problems, but also turns waste into treasure, increasing economic and social benefits.
  • the fermenting agent and the fermentation process for treating alcohol liquid in the present invention can completely solve the three waste problems of sugar factories and alcohol factories.
  • the fermentation process not only has a short process flow, but also has a low process cartridge cost, and has significant social and ecological benefits. Economic benefits.

Abstract

L'invention porte sur une préparation microbienne traitant les résidus alcooliques de distilleries et les mélasses de raffineries de sucre utilisant la flore WH, sur son procédé d'obtention et sur le procédé de fermentation associé. Ledit procédé de fermentation consiste à: effectuer une première fermentation des résidus alcooliques en leur ajoutant la préparation microbienne; faire fermenter la boue organique ainsi obtenue; mélanger les deux produits obtenus; et leur faire subir une nouvelle fermentation. On obtient ainsi un engrais organique. C'est donc un procédé simple et peu coûteux qui permet de résoudre certains problèmes d'environnement.
PCT/CN2003/000224 2002-04-30 2003-03-28 Preparation microbienne traitant les residus alcooliques, son procede d'obtention, et procede de fermentation associe WO2003093454A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003227171A AU2003227171A1 (en) 2002-04-30 2003-03-28 A microbe preparation for treating waste alcohol, its preparation method and the fermentation process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN02115197A CN1377958A (zh) 2002-04-30 2002-04-30 一种处理酒精废液的微生物发酵剂及其制备方法
CN02115197.0 2002-04-30

Publications (1)

Publication Number Publication Date
WO2003093454A1 true WO2003093454A1 (fr) 2003-11-13

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CN (1) CN1377958A (fr)
AU (1) AU2003227171A1 (fr)
WO (1) WO2003093454A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102616941A (zh) * 2012-03-26 2012-08-01 大连理工大学 一种海参微生态水质调节剂及其制备方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100391907C (zh) * 2005-11-03 2008-06-04 余超江 用蔗糖厂废弃的酒精废醪液生产有机肥的方法
CN100460345C (zh) * 2005-11-04 2009-02-11 中国地质大学（武汉） 耐氯菌株1号的应用
CN101475973B (zh) * 2009-01-20 2010-08-18 海南椰国食品有限公司 含酒精废液的细菌纤维素生产培养基
CN102160613A (zh) * 2010-02-22 2011-08-24 贝军 一种健胃、保肝、养生的生物制品及制作方法
CN102031233B (zh) * 2010-10-27 2013-09-04 中国烟草总公司湖北省公司 一种生物有机肥发酵菌种的制备方法
CN102943051B (zh) * 2010-10-27 2015-01-21 中国烟草总公司湖北省公司 一种生物有机肥发酵菌种s4的制备方法
CN104292019B (zh) * 2014-10-22 2017-06-23 云南元江县丰年农业发展有限公司 一种火龙果攻花期生物营养液的制备方法

Citations (4)

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JPS60248191A (ja) * 1984-05-21 1985-12-07 Satoshi Omura フオスフイノスリシンの製造方法
JPS63237781A (ja) * 1987-03-26 1988-10-04 Taki Chem Co Ltd 微生物菌体の製造方法
CN1254697A (zh) * 1999-11-08 2000-05-31 高明市绿宝植物营养制品有限公司 一种使作物秸秆腐烂的微生物制剂及其制备方法
JP2001178447A (ja) * 1999-12-24 2001-07-03 Shinohara Seiki Kk 農薬分解能を有する微生物群、該微生物群を含む堆肥とその製造方法及び装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60248191A (ja) * 1984-05-21 1985-12-07 Satoshi Omura フオスフイノスリシンの製造方法
JPS63237781A (ja) * 1987-03-26 1988-10-04 Taki Chem Co Ltd 微生物菌体の製造方法
CN1254697A (zh) * 1999-11-08 2000-05-31 高明市绿宝植物营养制品有限公司 一种使作物秸秆腐烂的微生物制剂及其制备方法
JP2001178447A (ja) * 1999-12-24 2001-07-03 Shinohara Seiki Kk 農薬分解能を有する微生物群、該微生物群を含む堆肥とその製造方法及び装置

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102616941A (zh) * 2012-03-26 2012-08-01 大连理工大学 一种海参微生态水质调节剂及其制备方法

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CN1377958A (zh) 2002-11-06
AU2003227171A1 (en) 2003-11-17

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