CN106310924A - Preparation method for biological deodorant of livestock farm - Google Patents
Preparation method for biological deodorant of livestock farm Download PDFInfo
- Publication number
- CN106310924A CN106310924A CN201610922990.3A CN201610922990A CN106310924A CN 106310924 A CN106310924 A CN 106310924A CN 201610922990 A CN201610922990 A CN 201610922990A CN 106310924 A CN106310924 A CN 106310924A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- conditions
- microbe
- biological deodorant
- livestock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/02—Other waste gases
- B01D2258/0266—Other waste gases from animal farms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention discloses a preparation method for a biological deodorant of livestock farm and belongs to the technical field of preparation of the biological deodorant. The method comprises the following steps: mixing pig manure, chicken manure, loess and deionized water and then inoculating with earthworm; cultivating; pouring earthworm cast into normal saline; stirring and dispersing, thus obtaining the upper suspension liquid; adding sodium sulfide wiith different gradient concentrations, screening and cultivating; collecting No.1 lower precipitate; adding ammonium hydroxide with different gradient concentrations, screening and cultivating; collecting No.2 lower precipitate; mixing the two lower precipitates; sealing and packaging; storing in a refrigerator, thereby obtaining the biological deodorant of livestock farm. According to the invention, the acquired biological deodorant of livestock farm can utilize the screened microorganism to effectively absorb and degrade the surrounding odor of the livestock farm; the biological deodorant is beneficial to the growth and development of livestock; the efficiency of feed utilization and the gaining capacity can be increased; the health hazard of harmful gas to livestock and human body can be reduced; the sustainable development of the livestock breeding industry is benefited.
Description
Technical field
The invention discloses the preparation method of a kind of fowl and livestock farm biological deodorant, belong to biological deodorant technology of preparing
Field.
Background technology
The stink substance that livestock culture industry produces is mainly derived from fecaluria and the feedstuff the like waste of waste of body excretion
The gas etc. that corrupt product, respiratory tract expellant gas and the porcine skin decomposed distributes.According to analyzing the stink contained in fecaluria
Compound reaches more than 168 kinds, in various foul gass, mainly includes nitrogen compound, sulfide, aliphatic compound, CO2、CH4
Deng, the normal cacodorous ammonia of fowl and livestock farm distributes, and can directly affect the growth promoter of poultry, and the ammonia of high concentration can destroy dynamic
Thing airway epithelial cell, adds the generation of respiratory tract disease, and it is left that the generation of typical pneumonia can make production cost increase by 10%
The right side, can make when ammonia concentration is more than 25ppm poultry produce coercion, the growth promoter of suppression poultry, reduce feed conversion
Rate and gain ability, during additionally toxic and harmful can enter feedstuff and water, cause the reduction of breeding performonce fo animals.Foul gas
Generation serious harm animal health and health, find safe and effective deodorizer, reduce the discharge of stink substance,
Become the key issue of livestock culture industry sustainable development.
At present, the deodorizer kind of research and development is the most both at home and abroad, and according to the difference of occupation mode, conventional substantially may be used
To be divided into spice encapsulation method, chemical deodorizing method and Ozonation.Wherein spice encapsulation method refers to by spraying in foul smell
Or volatilization spice, it is used for improving people's sensation to place air, and odorant pollutant is not occurred removal effect, therefore this method
Not claiming deodorizing method truly, the chemical deodorizing method of next is to utilize oxidation, reduction decomposition, neutralization reaction, addition
Generation odorant is become odorless material thus eliminates foul smell by reaction, condensation reaction, ion-exchange reactions etc., but the method
Investment is big, and operation cost is of a relatively high, and particularly chemical reaction afterproduct has the probability causing new uncontrollable environmental pollution,
The most also can cause human body diseases, last Ozonation is to be intensified in air by various methods (such as photocatalyst) to produce
O3, odorant pollutant in place is aoxidized, also has bactericidal action simultaneously, but the bigger defect of this method is ozone
Concentration is wayward, has substantially injury to health.The scale of China's animal husbandry, intensive culture universal, cultivation
During the foul smell that produces get more and more, severe contamination environment and have impact on the health of poultry self, but commonly use at present
Deodorizing method, generally there is deodorizing effect thorough, investment operation cost is high and containing substantial amounts of chemical composition, not only exists
Use reaction afterproduct to have and cause the environmental pollution that newly cannot control, and human body and poultry own health are had substantially
The drawback of injury.
Summary of the invention
The technical problem to be solved: the adsorption effect in use occurred for current conventional deodorizers
Difference, is heated after absorption and desorbing easily occurs, and causes secondary pollution, and not problem of easy degradation after using, it is provided that a kind of poultry is supported
Growing the preparation method of a biological deodorant, the present invention inoculates Lumbricus after utilizing pig manure, chicken manure, loess to mix with deionized water, enters
Row cultivation, then wormcast is poured in normal saline, dispersed with stirring obtains upper strata suspension, and adds variable concentrations gradient sodium sulfide
Carry out screening and culturing, collect No. 1 lower sediment thing, add variable concentrations gradient ammonia and carry out screening and culturing, collect No. 2
Lower sediment thing, finally by two kinds of lower sediment thing mixing, packs, is placed in refrigerator cold-storage, obtain fowl and livestock farm biological
Deodorizer.Gained fowl and livestock farm biological deodorant of the present invention utilizes the microorganism that screening obtains, and can effectively absorb and degrade fowl
Poultry plant surrounding enviroment foul smell, beneficially poultry growth promoter, improves efficiency of feed utilization and gain ability, reduces harmful gas
To poultry and human health damage, the beneficially sustainable development of livestock culture industry.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) weighing 300～500g pig manures, 200～300g chicken manures successively, 600～800g loess, with 300～400mL deionized waters
After mix homogeneously, being stacked at lucifuge ventilation, subsequently inoculation 80～120g Lumbricuss, carry out vermiculture 15～20 days, separation removes
Going Lumbricus, collect to obtain wormcast, weigh 60～80g gained wormcasts, pouring into and filling 300～400mL mass fractions is 0.9% life
In the triangular flask of reason saline, with Glass rod stirring mixing 15～20min, stand 10～15s, collect to obtain upper strata suspension；
(2) measure 60～80mL above-mentioned gained upper strata suspensions, pour 200～300mL microbe to screen culture medium into, add 10
～15g sodium sulfide, then culture medium is placed in shaking table, it is 30～32 DEG C in temperature, under the conditions of rotating speed is 100～120r/min,
Shaken cultivation 48～72h, treats that cultivation terminates, and pipettes 10～15mL culture fluid, pours 200～300mL new microbe screening and culturing into
In base, add 20～30g sodium sulfide, subsequently new microbe screening culture medium be placed in shaking table, be 30～32 DEG C in temperature,
Under the conditions of rotating speed is 100～120r/min, shaken cultivation 48～72h, repeats aforesaid operations, and sodium sulfide addition is increased to
30～45g, continue domestication cultivation 48～72h, centrifugation, discard the supernatant, collect to obtain No. 1 lower sediment thing, and by it
It is placed in refrigerator, cold preservation under the conditions of 4～6 DEG C, standby；
(3) measure 60～80mL step (1) gained upper strata suspensions, pour 200～300mL microbe to screen culture medium into, then add
Entering 10～15mL mass fractions is 15% ammonia, then culture medium is placed in shaking table, is 30～32 DEG C in temperature, rotating speed be 100～
Under the conditions of 120r/min, shaken cultivation 48～72h, treat that cultivation terminates, pipette 10～15mL culture fluid, pour 200～300mL into new
In microbe to screen culture medium, adding 20～30mL mass fractions is 15% ammonia, new microbe screening culture medium is put subsequently
In shaking table, it is 30～32 DEG C in temperature, under the conditions of rotating speed is 100～120r/min, shaken cultivation 48～72h, repeats above-mentioned
Operation, and ammonia addition is increased to 30～45mL, continue domestication cultivation 48～72h, centrifugation, discard the supernatant,
Collect to obtain No. 2 lower sediment things, and be placed in refrigerator, cold preservation under the conditions of 4～6 DEG C；
(4) count by weight, take 60～80 parts of standby No. 1 lower sediment things of step (2), 60～80 parts of above-mentioned gained 2 successively
Number lower sediment thing, packs the most afterwards, is placed in refrigerator, cold preservation under the conditions of 2～4 DEG C, obtains poultry and supports
Grow a biological deodorant.
Described microbe to screen culture medium is: wheaten starch 0.3～0.5%, molasses 3～5%, sulfur
Acid ammonium 0.15～0.36%, potassium dihydrogen phosphate 0.06～0.08%, magnesium sulfate 0.05～0.07%, remaining be deionized water, mix and
Become.
The application process of the present invention is: by gained fowl and livestock farm deodorizer of the present invention and water 1:100 in mass ratio～1:
200 are mixed into diluent, by 0.5～0.7kg/m2Diluent is sprayed to pig plant by usage amount, summer 1 times a week, monthly
Carrying out 4 times to spray, every 10 days of remaining season sprayed 1 time.After testing, gained fowl and livestock farm deodorizer of the present invention can make pig cultivate
In foul smell, ammonia level reduces by 90～95%, and hydrogen sulfide content reduces by 88～92%, the average removal rate of foul smell reach 88～
92%, make the rate of falling ill of pig reduce by 10～15%, adding weight improves 8～10%.
The invention has the beneficial effects as follows:
(1) gained fowl and livestock farm biological deodorant of the present invention in use will not produce harmful substance, can effectively absorb
And degrade fowl and livestock farm surrounding enviroment foul smell, beneficially poultry growth promoter, improve efficiency of feed utilization and gain ability, reduce
The harmful gas harm to health, the beneficially sustainable development of livestock culture industry；
(2) gained fowl and livestock farm biological deodorant processing technology of the present invention is simple, and raw materials for production are cheap and easy to get, deodorizing effect
Good, can large-scale promotion use.
Detailed description of the invention
Weighing 300～500g pig manures, 200～300g chicken manures successively, 600～800g loess, with 300～400mL deionizations
After water mix homogeneously, it is stacked at lucifuge ventilation, subsequently inoculation 80～120g Lumbricuss, carries out vermiculture 15～20 days, separating
Removing Lumbricus, collect to obtain wormcast, weigh 60～80g gained wormcasts, pouring into and filling 300～400mL mass fractions is 0.9%
In the triangular flask of normal saline, with Glass rod stirring mixing 15～20min, stand 10～15s, collect to obtain upper strata suspension；Amount
Take 60～80mL above-mentioned gained upper strata suspensions, pour 200～300mL microbe to screen culture medium into, add 10～15g sulfurations
Sodium, then culture medium is placed in shaking table, it is 30～32 DEG C in temperature, under the conditions of rotating speed is 100～120r/min, shaken cultivation 48
～72h, treat that cultivation terminates, pipette 10～15mL culture fluid, pour in 200～300mL new microbe screening culture medium, add
20～30g sodium sulfide, are placed in new microbe screening culture medium in shaking table subsequently, are 30～32 DEG C in temperature, rotating speed be 100～
Under the conditions of 120r/min, shaken cultivation 48～72h, repeats aforesaid operations, and sodium sulfide addition is increased to 30～45g, continue
Continuous domestication cultivation 48～72h, centrifugation, discard the supernatant, collect to obtain No. 1 lower sediment thing, and be placed in refrigerator,
Cold preservation under the conditions of 4～6 DEG C, measures 60～80mL gained upper strata suspensions, pours 200～300mL microbe to screen culture medium into,
Adding 10～15mL mass fractions is 15% ammonia, then culture medium is placed in shaking table, is 30～32 DEG C in temperature, and rotating speed is
Under the conditions of 100～120r/min, shaken cultivation 48～72h, treat that cultivation terminates, pipette 10～15mL culture fluid, pour into 200～
In 300mL new microbe screening culture medium, adding 20～30mL mass fractions is 15% ammonia, is screened by new microbe subsequently
Culture medium is placed in shaking table, is 30～32 DEG C in temperature, under the conditions of rotating speed is 100～120r/min, and shaken cultivation 48～72h,
Repeat aforesaid operations, and ammonia addition is increased to 30～45mL, continue domestication cultivation 48～72h, centrifugation, discard
Layer clear liquid, collects to obtain No. 2 lower sediment things, and is placed in refrigerator, cold preservation under the conditions of 4～6 DEG C；Count by weight,
Take 60～80 parts of No. 1 lower sediment things, No. 2 lower sediment things of 60～80 parts of above-mentioned gained, by evenly mixing rear sealed bundle successively
Dress, is placed in refrigerator, cold preservation under the conditions of 2～4 DEG C, obtains fowl and livestock farm biological deodorant.Described microbe to screen
Culture medium is by mass percentage by wheaten starch 0.3～0.5%, molasses 3～5%, ammonium sulfate 0.15～0.36%, biphosphate
Potassium 0.06～0.08%, magnesium sulfate 0.05～0.07%, remaining is deionized water, mixes.
Example 1
Weigh 300g pig manure, 200g chicken manure, 600g loess successively, after mixing homogeneously with 300mL deionized water, be stacked at lucifuge and lead to
At wind, inoculation 80g Lumbricus, carries out vermiculture 15 days, is separated off Lumbricus, collect to obtain wormcast, weigh 60g gained earthworm subsequently
Earthworm excrement, pours into and fills in the triangular flask that 300mL mass fraction is 0.9% normal saline, with Glass rod stirring mixing 15min, stands
10s, collects to obtain upper strata suspension；Measure 60mL above-mentioned gained upper strata suspension, pour 200mL microbe to screen culture medium into, then
Add 10g sodium sulfide, then culture medium is placed in shaking table, be 30 DEG C in temperature, under the conditions of rotating speed is 100r/min, shaken cultivation
48h, treats that cultivation terminates, and pipettes 10mL culture fluid, pours in 200mL new microbe screening culture medium, adds 20g sodium sulfide,
Subsequently new microbe screening culture medium is placed in shaking table, is 30 DEG C in temperature, under the conditions of rotating speed is 100r/min, shaken cultivation
48h, repeats aforesaid operations, and sodium sulfide addition increases to 30g, continues domestication and cultivates 48h, centrifugation, discards upper strata
Clear liquid, collects to obtain No. 1 lower sediment thing, and is placed in refrigerator, cold preservation under the conditions of 4 DEG C, measures 60mL gained upper strata and hangs
Supernatant liquid, pours 200mL microbe to screen culture medium into, and adding 10mL mass fraction is 15% ammonia, then culture medium is placed in shaking table
In, it is 30 DEG C in temperature, under the conditions of rotating speed is 100r/min, shaken cultivation 48h, treat that cultivation terminates, pipette 10mL culture fluid, fall
Entering in 200mL new microbe screening culture medium, adding 20mL mass fraction is 15% ammonia, subsequently by new microbe screening training
Foster base is placed in shaking table, is 30 DEG C in temperature, under the conditions of rotating speed is 100r/min, and shaken cultivation 48h, repeat aforesaid operations, and
Ammonia addition is increased to 30mL, continues domestication and cultivate 48h, centrifugation, discard the supernatant, collect No. 2 lower floors sink
Shallow lake thing, and be placed in refrigerator, cold preservation under the conditions of 4 DEG C；Count by weight, take 60 parts of No. 1 lower sediment things successively, 60
Part No. 2 lower sediment things of above-mentioned gained, pack the most afterwards, are placed in refrigerator, cold preservation under the conditions of 2 DEG C, i.e.
Obtain fowl and livestock farm biological deodorant.Described microbe to screen culture medium is by mass percentage by wheaten starch 0.3%, sugar
Honey 3%, ammonium sulfate 0.15%, potassium dihydrogen phosphate 0.06%, magnesium sulfate 0.05%, remaining is deionized water, mixes.
Gained fowl and livestock farm deodorizer of the present invention is mixed into diluent, by 0.5kg/m with water 1:100 in mass ratio2
Diluent is sprayed to pig plant by usage amount, and summer 1 times a week, monthly carries out 4 times and sprays, and every 10 days of remaining season sprayed 1
Secondary.After testing, gained fowl and livestock farm deodorizer of the present invention can reduce by 90% by ammonia level in Shi Zhu plant foul smell, hydrogen sulfide
Content reduces by 88%, and the average removal rate of foul smell reaches 88%, makes the rate of falling ill of pig reduce by 10%, and adding weight improves 8%.
Example 2
Weigh 500g pig manure, 300g chicken manure, 800g loess successively, after mixing homogeneously with 400mL deionized water, be stacked at lucifuge and lead to
At wind, inoculation 20g Lumbricus, carries out vermiculture 20 days, is separated off Lumbricus, collect to obtain wormcast, weigh 80g gained earthworm subsequently
Earthworm excrement, pours into and fills in the triangular flask that 400mL mass fraction is 0.9% normal saline, with Glass rod stirring mixing 20min, stands
15s, collects to obtain upper strata suspension；Measure 80mL above-mentioned gained upper strata suspension, pour 300mL microbe to screen culture medium into, then
Add 15g sodium sulfide, then culture medium is placed in shaking table, be 32 DEG C in temperature, under the conditions of rotating speed is 120r/min, shaken cultivation
72h, treats that cultivation terminates, and pipettes 15mL culture fluid, pours in 300mL new microbe screening culture medium, adds 30g sodium sulfide,
Subsequently new microbe screening culture medium is placed in shaking table, is 32 DEG C in temperature, under the conditions of rotating speed is 120r/min, shaken cultivation
72h, repeats aforesaid operations, and sodium sulfide addition increases to 45g, continues domestication and cultivates 72h, centrifugation, discards upper strata
Clear liquid, collects to obtain No. 1 lower sediment thing, and is placed in refrigerator, cold preservation under the conditions of 6 DEG C, measures 80mL gained upper strata and hangs
Supernatant liquid, pours 300mL microbe to screen culture medium into, and adding 15mL mass fraction is 15% ammonia, then culture medium is placed in shaking table
In, it is 32 DEG C in temperature, under the conditions of rotating speed is 120r/min, shaken cultivation 72h, treat that cultivation terminates, pipette 15mL culture fluid, fall
Entering in 300mL new microbe screening culture medium, adding 30mL mass fraction is 15% ammonia, subsequently by new microbe screening training
Foster base is placed in shaking table, is 32 DEG C in temperature, under the conditions of rotating speed is 120r/min, and shaken cultivation 72h, repeat aforesaid operations, and
Ammonia addition is increased to 45mL, continues domestication and cultivate 72h, centrifugation, discard the supernatant, collect No. 2 lower floors sink
Shallow lake thing, and be placed in refrigerator, cold preservation under the conditions of 6 DEG C；Count by weight, take 80 parts of No. 1 lower sediment things successively,
No. 2 lower sediment things of 80 parts of above-mentioned gained, pack the most afterwards, are placed in refrigerator, cold preservation under the conditions of 4 DEG C,
Obtain fowl and livestock farm biological deodorant.Described microbe to screen culture medium is by mass percentage by wheaten starch 0.5%,
Molasses 5%, ammonium sulfate 0.36%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.07%, remaining is deionized water, mixes.
Gained fowl and livestock farm deodorizer of the present invention is mixed into diluent, by 0.7kg/m with water 1:200 in mass ratio2
Diluent is sprayed to pig plant by usage amount, and summer 1 times a week, monthly carries out 4 times and sprays, and every 10 days of remaining season sprayed 1
Secondary.After testing, gained fowl and livestock farm deodorizer of the present invention can reduce by 95% by ammonia level in Shi Zhu plant foul smell, hydrogen sulfide
Content reduces by 92%, and the average removal rate of foul smell reaches 92%, makes the rate of falling ill of pig reduce by 15%, and adding weight improves 10%.
Example 3
Weigh 400g pig manure, 250g chicken manure, 700g loess successively, after mixing homogeneously with 350mL deionized water, be stacked at lucifuge and lead to
At wind, inoculation 100g Lumbricus, carries out vermiculture 18 days, is separated off Lumbricus, collect to obtain wormcast, weigh 70g gained subsequently
Wormcast, pours into and fills in the triangular flask that 350mL mass fraction is 0.9% normal saline, with Glass rod stirring mixing 18min, quiet
Put 135s, collect to obtain upper strata suspension；Measure 70mL above-mentioned gained upper strata suspension, pour 250mL microbe to screen culture medium into,
Add 13g sodium sulfide, then culture medium is placed in shaking table, be 31 DEG C in temperature, under the conditions of rotating speed is 110r/min, vibration training
Support 60h, treat that cultivation terminates, pipette 13mL culture fluid, pour in 250mL new microbe screening culture medium, add 25g sulfuration
Sodium, is placed in new microbe screening culture medium in shaking table subsequently, is 31 DEG C in temperature, under the conditions of rotating speed is 110r/min, and vibration
Cultivate 60h, repeat aforesaid operations, and sodium sulfide addition is increased to 38g, continue domestication and cultivate 60h, centrifugation, discard
The supernatant, collects to obtain No. 1 lower sediment thing, and is placed in refrigerator, cold preservation under the conditions of 5 DEG C, measure on 70mL gained
Layer suspension, pours 250mL microbe to screen culture medium into, and adding 13mL mass fraction is 15% ammonia, then culture medium is placed in
In shaking table, it is 31 DEG C in temperature, under the conditions of rotating speed is 110r/min, shaken cultivation 60h, treat that cultivation terminates, pipette 13mL and cultivate
Liquid, pours in 250mL new microbe screening culture medium, and adding 25mL mass fraction is 15% ammonia, is sieved by new microbe subsequently
Select culture medium to be placed in shaking table, be 31 DEG C in temperature, under the conditions of rotating speed is 110r/min, shaken cultivation 60h, repeats above-mentioned behaviour
Make, and ammonia addition increased to 38mL, continue domestication and cultivate 60h, centrifugation, discard the supernatant, collect under No. 2
Layer precipitate, and be placed in refrigerator, cold preservation under the conditions of 5 DEG C；Count by weight, take 70 parts of No. 1 lower sediment successively
Thing, No. 2 lower sediment things of 70 parts of above-mentioned gained, pack the most afterwards, be placed in refrigerator, cold under the conditions of 3 DEG C
Hide, obtain fowl and livestock farm biological deodorant.Described microbe to screen culture medium is by mass percentage by wheaten starch
0.4%, molasses 4%, ammonium sulfate 0.25%, potassium dihydrogen phosphate 0.07%, magnesium sulfate 0.06%, remaining is deionized water, mixes.
Gained fowl and livestock farm deodorizer of the present invention is mixed into diluent, by 0.6kg/m with water 1:150 in mass ratio2
Diluent is sprayed to pig plant by usage amount, and summer 1 times a week, monthly carries out 4 times and sprays, and every 10 days of remaining season sprayed 1
Secondary.After testing, gained fowl and livestock farm deodorizer of the present invention can reduce by 93% by ammonia level in Shi Zhu plant foul smell, hydrogen sulfide
Content reduces by 90%, and the average removal rate of foul smell reaches 90%, makes the rate of falling ill of pig reduce by 13%, and adding weight improves 9%.
Claims (2)
1. the preparation method of a fowl and livestock farm biological deodorant, it is characterised in that concrete preparation process is:
(1) weighing 300～500g pig manures, 200～300g chicken manures successively, 600～800g loess, with 300～400mL deionized waters
After mix homogeneously, being stacked at lucifuge ventilation, subsequently inoculation 80～120g Lumbricuss, carry out vermiculture 15～20 days, separation removes
Going Lumbricus, collect to obtain wormcast, weigh 60～80g gained wormcasts, pouring into and filling 300～400mL mass fractions is 0.9% life
In the triangular flask of reason saline, with Glass rod stirring mixing 15～20min, stand 10～15s, collect to obtain upper strata suspension；
(2) measure 60～80mL above-mentioned gained upper strata suspensions, pour 200～300mL microbe to screen culture medium into, add 10
～15g sodium sulfide, then culture medium is placed in shaking table, it is 30～32 DEG C in temperature, under the conditions of rotating speed is 100～120r/min,
Shaken cultivation 48～72h, treats that cultivation terminates, and pipettes 10～15mL culture fluid, pours 200～300mL new microbe screening and culturing into
In base, add 20～30g sodium sulfide, subsequently new microbe screening culture medium be placed in shaking table, be 30～32 DEG C in temperature,
Under the conditions of rotating speed is 100～120r/min, shaken cultivation 48～72h, repeats aforesaid operations, and sodium sulfide addition is increased to
30～45g, continue domestication cultivation 48～72h, centrifugation, discard the supernatant, collect to obtain No. 1 lower sediment thing, and by it
It is placed in refrigerator, cold preservation under the conditions of 4～6 DEG C, standby；
(3) measure 60～80mL step (1) gained upper strata suspensions, pour 200～300mL microbe to screen culture medium into, then add
Entering 10～15mL mass fractions is 15% ammonia, then culture medium is placed in shaking table, is 30～32 DEG C in temperature, rotating speed be 100～
Under the conditions of 120r/min, shaken cultivation 48～72h, treat that cultivation terminates, pipette 10～15mL culture fluid, pour 200～300mL into new
In microbe to screen culture medium, adding 20～30mL mass fractions is 15% ammonia, new microbe screening culture medium is put subsequently
In shaking table, it is 30～32 DEG C in temperature, under the conditions of rotating speed is 100～120r/min, shaken cultivation 48～72h, repeats above-mentioned
Operation, and ammonia addition is increased to 30～45mL, continue domestication cultivation 48～72h, centrifugation, discard the supernatant,
Collect to obtain No. 2 lower sediment things, and be placed in refrigerator, cold preservation under the conditions of 4～6 DEG C；
(4) count by weight, take 60～80 parts of standby No. 1 lower sediment things of step (2), 60～80 parts of above-mentioned gained 2 successively
Number lower sediment thing, packs the most afterwards, is placed in refrigerator, cold preservation under the conditions of 2～4 DEG C, obtains poultry and supports
Grow a biological deodorant.
The preparation method of a kind of fowl and livestock farm biological deodorant the most according to claim 1, it is characterised in that described
Microbe to screen culture medium is: wheaten starch 0.3～0.5%, molasses 3～5%, ammonium sulfate 0.15～
0.36%, potassium dihydrogen phosphate 0.06～0.08%, magnesium sulfate 0.05～0.07%, remaining is deionized water, mixes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610922990.3A CN106310924A (en) | 2016-10-29 | 2016-10-29 | Preparation method for biological deodorant of livestock farm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610922990.3A CN106310924A (en) | 2016-10-29 | 2016-10-29 | Preparation method for biological deodorant of livestock farm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106310924A true CN106310924A (en) | 2017-01-11 |
Family
ID=57818341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610922990.3A Pending CN106310924A (en) | 2016-10-29 | 2016-10-29 | Preparation method for biological deodorant of livestock farm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106310924A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059331A (en) * | 1990-08-28 | 1992-03-11 | 东邦科技株式会社 | The deodorizing method of dung and urine of breeding animals |
| CN1559617A (en) * | 2004-03-03 | 2005-01-05 | 中国农业大学 | Compound deodorant membrane and its preparation method |
| CN1608456A (en) * | 2003-10-22 | 2005-04-27 | 中国农业大学 | Earthworm excrement deodorizing method and apparatus |
| CN1721057A (en) * | 2005-06-18 | 2006-01-18 | 武刚 | Air purificant and its preparation method |
| WO2007046650A1 (en) * | 2005-10-22 | 2007-04-26 | Se Joon Park | Microorganisms having bad smell removal activity of organic waste and use thereof |
| CN105079842A (en) * | 2015-09-15 | 2015-11-25 | 江苏锦宇环境工程有限公司 | Preparation method of poultry farm deodorant |
| CN105152700A (en) * | 2015-08-03 | 2015-12-16 | 刘雷 | Organic garbage deodorant based on earthworm cast and compound microorganisms |
-
2016
- 2016-10-29 CN CN201610922990.3A patent/CN106310924A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059331A (en) * | 1990-08-28 | 1992-03-11 | 东邦科技株式会社 | The deodorizing method of dung and urine of breeding animals |
| CN1608456A (en) * | 2003-10-22 | 2005-04-27 | 中国农业大学 | Earthworm excrement deodorizing method and apparatus |
| CN1559617A (en) * | 2004-03-03 | 2005-01-05 | 中国农业大学 | Compound deodorant membrane and its preparation method |
| CN1721057A (en) * | 2005-06-18 | 2006-01-18 | 武刚 | Air purificant and its preparation method |
| WO2007046650A1 (en) * | 2005-10-22 | 2007-04-26 | Se Joon Park | Microorganisms having bad smell removal activity of organic waste and use thereof |
| CN105152700A (en) * | 2015-08-03 | 2015-12-16 | 刘雷 | Organic garbage deodorant based on earthworm cast and compound microorganisms |
| CN105079842A (en) * | 2015-09-15 | 2015-11-25 | 江苏锦宇环境工程有限公司 | Preparation method of poultry farm deodorant |
Non-Patent Citations (4)
| Title |
|---|
| 何明清等: "猪微生物饲料添加剂(8701)对生长育肥猪抗病、增重及提高饲料利用率研究", 《中国生态学杂志》 * |
| 周东兴等: "蚯蚓粪中异养脱硫菌筛选及效果测定", 《东北农业大学学报》 * |
| 尚丽勤: "蚯蚓粪便微生物多样性的研究及除臭微生物的分离筛选", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
| 曾苏等: "微生物除臭剂的筛选、复配及其除臭条件的优化", 《环境科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103933595B (en) | Compound recipe deodorizer and using method thereof | |
| CN105272476A (en) | Livestock and poultry excrement composting deodorant | |
| CN210186866U (en) | Odor purification system in livestock and poultry farm house | |
| CN105624072A (en) | Active microbial agent for deodorizing livestock and poultry feces and preparation method thereof | |
| CN113207710B (en) | Probiotic cat litter capable of deodorizing and inhibiting bacteria and preparation method thereof | |
| CN106187521B (en) | A kind of feces of livestock and poultry deodorant | |
| CN107601764A (en) | A kind of livestock and poultry cultivation fecal pollution is decomposed and utilizes technique | |
| CN104222506A (en) | Environmentally-friendly pig breeding method | |
| CN110170247A (en) | A kind of livestock and poultry farm give up in odor purification system and its application method | |
| CN106946424A (en) | Dirty plant source deodorant of a kind of pig farm excrement and its preparation method and application | |
| JPH04160085A (en) | Fermented product and its production | |
| CN104987214A (en) | Efficient biology leavening agent capable of decreasing pollution gas emission in urine immersing manure technology | |
| CN106187458A (en) | Swine manure and stalk fermentation compost method | |
| CN105800888B (en) | A kind of poultry farms treatment for cow manure method | |
| RU2587549C1 (en) | Method for vermiculture of chicken manure | |
| CN110041151A (en) | A kind of harmless treatment process for waste of breeding silkworms | |
| CN103041423A (en) | Water lettuce deodorant | |
| CN108299032A (en) | A method of quickly preparing organic fertilizer | |
| CN111925240A (en) | Nitrogen-preserving deodorant for high-temperature composting of livestock and poultry manure | |
| JP3187495U (en) | Composting equipment and livestock equipment using deodorizing materials | |
| CN101439196A (en) | Nitrogen-protecting deodorant for faeces of pig and method for producing the same | |
| CN106310924A (en) | Preparation method for biological deodorant of livestock farm | |
| CN106495762B (en) | A kind of pig manure method for reducing pollutant emission and improving yield | |
| TW201813514A (en) | Biological agents to improve the growth of pigs and farms environment and use thereof | |
| CN112516784A (en) | Preparation method of biological deodorant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |