CN105152697A - Organic fertilizer prepared from solidago canadensis and platanus orientalis leaves - Google Patents
Organic fertilizer prepared from solidago canadensis and platanus orientalis leaves Download PDFInfo
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- CN105152697A CN105152697A CN201510448237.0A CN201510448237A CN105152697A CN 105152697 A CN105152697 A CN 105152697A CN 201510448237 A CN201510448237 A CN 201510448237A CN 105152697 A CN105152697 A CN 105152697A
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Abstract
The invention provides an organic fertilizer prepared from solidago canadensis and platanus orientalis leaves, and relates to a novel organic fertilizer with excellent fertility. The fertilizer is prepared from using solidago canadensis and platanus orientalis leaves as fundamental raw materials in a mass ratio of 1 to1 by adopting a solid fermentation method. The organic fertilizer can be used for remarkably increasing the yield of crops, preventing and treating sclerotinia rot of colza to a certain extent.
Description
Technical field
The present invention relates to fertilizer field, especially organic fertilizer technical field.
Background technology
Organic substance in organic fertilizer and nutritive substance are all very abundant, can not only provide required nutrient, can also improve crop yield, improve crop quality, preservation of fertility for the growth of crop, can improve utilization rate of fertilizer simultaneously, reduce production cost.
Along with the raising of people's living standard, the demand of free of contamination organic, the green food of safety and sanitation is in continuous increase, and vast farmers improves the market competitiveness of agricultural-food in the urgent need to application of organic fertilizers.At present, western countries' organic fertilizer consumptions such as the U.S. have accounted for 50% of total amount, and China's Organic fertilizer industry merchandized handling begins to take shape, and using on a large scale of farmland is not yet universal, and institute's accounting is less than 10%.
Rape is the main oil crops of China, and sclerotinia rot of colza is one of important disease in Rape-seed production, and long-term strain sickness rate is up to 10% ~ 30%, and serious reaches more than 80%; The general underproduction 10% ~ 70% of diseased plant.
The Pathogenic Strains sclerotinite (Sclerotiniasclerotiorum (Lib.) deBary) of sclerotinia rot of colza, belongs to Ascomycotina fungi.Sclerotium Long Circle, to irregular shape, like mouse excrement shape, grays after first white, inner canescence.Grow 1 after sclerotial germination to the meat tawny plate-like apothecium of multiple tool long handle, on dish raw one deck ascus and lateral filament, the colourless club-like of ascus, include the colourless thecaspore of unit cell 8, lateral filament is colourless, thread, half-cooked between ascus.
Solidago canadesis (SolidagoCanadensisLour.), is named again North America Herba Solidaginis, is composite family per nnial herb per nnial herb, Solidago, high 150 ~ the 300cm of plant, close upright by fine hair stem, not branch or few branch.Leaf alternate, carefully close, two sides is by undercoat; Blade grows back shape lanceolar.Tip is gradually sharp, and base portion is gradually narrow; Leaf margin has shallow sawtooth, and increment is papillule shape; There are two obvious veins at downward 2/3 place of blade; Petiole is extremely short. do not embrace stem. and 4 ~ November of vegetative period.Solidago canadesis is bred by root and seed two kinds of modes, has superpower fecundity, 3 years just can be in blocks rapidly, and other crops in its vitellarium are withered away without exception.As external infringement species, it should not be underestimated to the destruction of agriculture production.
Sycamore (PlatanusorientalisLinn.), also known as oriental plant, Gao Keda 30 meters is world-renowned excellent shade tree and shade tree, has the title of " king of shade tree ".Its tree crown is wealthy bell; Dry hide beige, to canescence, is peeled off in flake.The close raw brown stellate hair of sprout, spire.Leaf palmate 5-7 splits, and drastic crack reaches middle part, and sliver is grown up in wide, the wealthy wedge shape of phyllopodium or section shape, and leaf margin has teeth, palmate vein; Stipule crew neck shape.Inflorescence head, yellow-green colour.Most nut gathers full leaf-head shape, and 3-6 ball becomes a string, and it is long that style is deposited in place, in setiform, and carpopodium long and nodding.In prior art, the leaf of sycamore is usually abandoned by as forestry waste, has not only polluted environment but also has caused the waste of resource.
Whiterot fungi (Phanerochaetechrysosporium), bacterium number is: CICC14076.
Healthy and free from worry wood mould (Trichodermakoningii), bacterium number is: CICC40108
In prior art, not yet useful solidago canadesis and sycamore leaf are the report that main raw material prepares organic fertilizer, more not with the report of this fertilizer control sclerotinia rot of colza.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind ofly can prevent and treat sclerotinia rot of colza and the organic fertilizer of fertility excellence.
In order to solve the problems of the technologies described above, the present invention proposes following technical proposal:
Described organic fertilizer take mass ratio as raw material based on the solidago canadesis of 1:1 and sycamore leaf, adopts solid fermentation method preparation and obtain.
Described solid fermentation method comprises the following steps:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: get described basic material, pulverize, insert 70-90 DEG C of baking oven inner drying, the ratio adding 300-400g water and 1-2g glucose in every 100g basic material adds water and glucose, mixes, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 10-30mL in every 100g basic material is inoculated, and cultivates 6-12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Strain expanded culture liquid PDA substratum used is the PDA substratum not adding agar, its preparation method takes 200g potato, clean peeling is cut into small pieces, the 1000ml that adds water boils half hour or steaming and decocting under high pressure 20 minutes, filtered through gauze, then add 10-20g glucose, filtered through gauze while hot after fully dissolving, packing test tube, after about sterilizing, taking-up storage is for subsequent use.
Preferably, described (2) step, the drying temperature of basic material is 80 DEG C.
Preferably, described (2) step, the quality that every 100g basic material adds water is 350g.
Preferably, described (2) step, the quality that every 100g basic material adds glucose is 1.5g.
Preferably, described (3) step, the volume that every 100g basic material adds containing bacterium liquid is 20mL.This
The beneficial effect of the invention can be proved by following test
Test example 1 manure trial
Material and reagent
For examination fertilizer: organic fertilizer 1 (being designated as examination group 1) of the present invention, prepare by embodiment 1; Organic fertilizer 2 (being designated as examination group 2) of the present invention, prepares by embodiment 2; Organic fertilizer 3 (being designated as examination group 3) of the present invention, prepares by embodiment 3.
Shanghai heat resistanceheat resistant 605 green vegetables seed.
Test method
Adopt randomized block design method design pot experiment.Get volume identical, the flowerpot that shape is consistent, be divided into for examination group (1,2,3) and blank group.For trying fertilizer according to clay fertilizer than 7:3 and mixing with soil, will irrigate with water after loading flowerpot, as supplying examination group (1,2,3); Blank group does not add fertilizer.Often organize test 10 basin, every basin is sowed 9 Shanghai heat resistanceheat resistant 605 seeds and (is considered the percentage of germination of seed, the point that 3 spacing are identical is fixed in flowerpot, each point puts 3 seeds, thinning after germinateing, each point only retains one and grows, and the consistent seedling of growing way), after planting sprinkle one deck dirt (form through high-temperature firing, prevent seed contamination) alive.
Cultivation Shanghai heat resistanceheat resistant 605 green vegetables of 40 days are plucked and weighs, record the growing state of each group of Shanghai heat resistanceheat resistant 605 green vegetables.
Test-results
Test-results is in table 1, and result shows, organic fertilizer of the present invention can improve the output of crop.
The impact that table 1 organic fertilizer of the present invention grows Shanghai heat resistanceheat resistant 605 green vegetables
| Process | Plant height (cm) | Strain heavy (g) | Degree of development (cm) | The number of blade |
| For examination group 1 | 24.9 | 91.3 | 26.2 | 13.3 |
| For examination group 2 | 22.8 | 85.9 | 25.7 | 12.4 |
| For examination group 3 | 25.7 | 90.4 | 26.9 | 13.1 |
| Blank group | 20.3 | 82.5 | 23.4 | 10.2 |
Test example 2 is on the impact of rape sclerotinite sclerotial germination rate
Material and reagent
For examination fertilizer: organic fertilizer 1 (being designated as examination group 1) of the present invention, prepare by embodiment 1; Organic fertilizer 2 (being designated as examination group 2) of the present invention, prepares by embodiment 2; Organic fertilizer 3 (being designated as examination group 3) of the present invention, prepares by embodiment 3.
Rape sclerotinite (Sclerotiniasclerotiorum (Lib.) deBary).
Test method
Adopt randomized block design method design pot experiment.Get volume identical, the flowerpot that shape is consistent, be divided into for examination group (1,2,3) and blank group.For trying fertilizer according to clay fertilizer than 7:3 and mixing with soil, will irrigate with water after loading flowerpot, as supplying examination group (1,2,3); Blank group does not add fertilizer.Often organize test 3 basin, load 21 sclerotinia rot of colza sclerotium, length of embedment 2cm with the little mesh bag 5cm × 5cm of nylon, when 60 days, measure the germination rate of sclerotium with potato sucrose substratum (PDA).
Test-results
Test-results is in table 2, and result shows, organic fertilizer of the present invention significantly can reduce the germination rate of rape nuclear disk sclerotium, has certain preventive and therapeutic effect to sclerotinia rot of colza.
Table 2 organic fertilizer of the present invention is on the impact of nuclear disk sclerotium germination rate
| Process | Germination rate (%) |
| For examination group 1 | 27.1 |
| For examination group 2 | 26.3 |
| For examination group 3 | 26.6 |
| Control group | 36.7 |
In order to better set forth technical scheme of the present invention, below in conjunction with embodiment, the present invention is further illustrated, but protection domain of the presently claimed invention is not limited to the following example.
Embodiment
Embodiment 1
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: take 1 part of solidago canadesis and 1 part of sycamore leaf by quality, pulverize, insert 70-90 DEG C of baking oven inner drying, the ratio adding 300g water and 1g glucose in every 100g basic material adds water and glucose, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 10mL in every 100g basic material is inoculated, cultivates 6 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Embodiment 2
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: take 1 part of solidago canadesis and 1 part of sycamore leaf by quality, pulverize, insert 90 DEG C of baking oven inner dryings, the ratio adding 400g water and 2g glucose in every 100g basic material adds water and glucose, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 30mL in every 100g basic material is inoculated, cultivates 12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Embodiment 3
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: take 1 part of solidago canadesis and 1 part of sycamore leaf by quality, pulverize, insert 80 DEG C of baking oven inner dryings, the ratio adding 350g water and 1.5g glucose in every 100g basic material adds water and glucose, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20mL in every 100g basic material is inoculated, cultivates 8 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Claims (6)
1. the organic fertilizer made with solidago canadesis and sycamore leaf, it is characterized in that, described organic fertilizer take mass ratio as raw material based on the solidago canadesis of 1:1 and sycamore leaf, adopts solid fermentation method preparation and obtain.
2. a kind of organic fertilizer according to claim 1, it is characterized in that, described solid fermentation method comprises the following steps:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: get described basic material, pulverize, insert 70-90 DEG C of baking oven inner drying, the ratio adding 300-400g water and 1-2g glucose in every 100g basic material adds water and glucose, mixes, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 10-30mL in every 100g basic material is inoculated, and cultivates 6-12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
3. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the drying temperature of basic material is 80 DEG C.
4. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the quality that every 100g basic material adds water is 350g.
5. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the quality that every 100g basic material adds glucose is 1.5g.
6. a kind of organic fertilizer according to claim 2, it is characterized in that, described (3) step, the volume that every 100g basic material adds containing bacterium liquid is 20mL.
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| CN201510448237.0A CN105152697A (en) | 2015-07-24 | 2015-07-24 | Organic fertilizer prepared from solidago canadensis and platanus orientalis leaves |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115399216A (en) * | 2021-05-26 | 2022-11-29 | 南京农业大学 | Improved clay loam based on sycamore fallen leaves as well as preparation method and application thereof |
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| CN104355832A (en) * | 2014-11-06 | 2015-02-18 | 张爱丽 | Water chestnut fertilizer and preparation method thereof |
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2015
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Patent Citations (5)
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| CN102229449A (en) * | 2011-06-28 | 2011-11-02 | 上海楚水水产科技有限公司 | Microbial amino acid rich water fertilizer product and preparation method thereof |
| CN102649654A (en) * | 2012-04-25 | 2012-08-29 | 孟凡尧 | Traditional Chinese medicine organic fertilizer |
| CN103274808A (en) * | 2013-04-24 | 2013-09-04 | 陆玉 | Bio-organic fertilizer with putrid vegetable leaves putrid vegetable leaves as main material, and preparation method thereof |
| CN104276896A (en) * | 2014-10-24 | 2015-01-14 | 国家粳稻工程技术研究中心 | Ecological medicinal fertilizer for rice and preparation method of ecological medicinal fertilizer |
| CN104355832A (en) * | 2014-11-06 | 2015-02-18 | 张爱丽 | Water chestnut fertilizer and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115399216A (en) * | 2021-05-26 | 2022-11-29 | 南京农业大学 | Improved clay loam based on sycamore fallen leaves as well as preparation method and application thereof |
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Application publication date: 20151216 |