CN105143249A - 通过结构互补激活生物发光 - Google Patents

通过结构互补激活生物发光 Download PDF

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Publication number
CN105143249A
CN105143249A CN201480015213.XA CN201480015213A CN105143249A CN 105143249 A CN105143249 A CN 105143249A CN 201480015213 A CN201480015213 A CN 201480015213A CN 105143249 A CN105143249 A CN 105143249A
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CN
China
Prior art keywords
peptide
polypeptide
sequence
luminous
noclilucence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480015213.XA
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English (en)
Chinese (zh)
Inventor
A·S·狄克逊
L·恩塞尔
M·霍尔
K·伍德
M·伍德
M·施温
B·F·宾科夫斯基
H·泽格祖蒂
N·纳思
S·蒙达尔
S·古埃利
P·迈森海默
T·柯克兰
J·昂切
D·K·普卢克库纳特
M·罗贝斯
M·达特
T·马赫莱特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pu Luomaige Co
Promega Corp
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Pu Luomaige Co
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Filing date
Publication date
Application filed by Pu Luomaige Co filed Critical Pu Luomaige Co
Priority to CN202310649630.0A priority Critical patent/CN117024548A/zh
Publication of CN105143249A publication Critical patent/CN105143249A/zh
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04Organic compounds
    • A61K51/08Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43509Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from crustaceans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/02Linear peptides containing at least one abnormal peptide link
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/08Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Optics & Photonics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Insects & Arthropods (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480015213.XA 2013-03-15 2014-03-13 通过结构互补激活生物发光 Pending CN105143249A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310649630.0A CN117024548A (zh) 2013-03-15 2014-03-13 通过结构互补激活生物发光

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361791549P 2013-03-15 2013-03-15
US61/791,549 2013-03-15
PCT/US2014/026354 WO2014151736A1 (en) 2013-03-15 2014-03-13 Activation of bioluminescence by structural complementation

Related Child Applications (1)

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Publications (1)

Publication Number Publication Date
CN105143249A true CN105143249A (zh) 2015-12-09

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US (8) US9797889B2 (es)
EP (4) EP4177261A1 (es)
JP (3) JP6654557B2 (es)
KR (2) KR20230079494A (es)
CN (2) CN105143249A (es)
AU (3) AU2014236949C1 (es)
BR (1) BR112015023394B8 (es)
CA (1) CA2906063A1 (es)
DK (2) DK3783011T3 (es)
ES (2) ES2926463T3 (es)
IL (3) IL286816B (es)
PL (2) PL3783011T3 (es)
PT (2) PT2970412T (es)
SG (2) SG10201601929YA (es)
WO (1) WO2014151736A1 (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9797889B2 (en) 2013-03-15 2017-10-24 Promega Corporation Activation of bioluminescence by structural complementation
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
WO2016040835A1 (en) * 2014-09-12 2016-03-17 Promega Corporation Internal protein tags
EP3254113A4 (en) 2015-02-05 2018-06-27 Promega Corporation Luciferase-based thermal shift assays
WO2017189751A1 (en) 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
DK3529616T3 (da) 2016-10-20 2023-12-11 Harvard College In vitro- og cellebaserede assays til måling af aktiviteten af botulinum-neurotoksiner
CA3022981A1 (en) * 2017-11-01 2019-05-01 Queen's University At Kingston Hippo pathway bioluminescent biosensor
PL3759233T4 (pl) * 2018-02-26 2022-10-10 Technische Universiteit Eindhoven Bioczujnik bioluminescencyjny do wykrywania i oznaczania ilościowego biocząsteczek
WO2019232384A1 (en) 2018-06-01 2019-12-05 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
JP2021526832A (ja) * 2018-06-12 2021-10-11 プロメガ コーポレイションPromega Corporation 多分子ルシフェラーゼ
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
BR112021006298A2 (pt) 2018-10-03 2021-07-06 Promega Corp composições e métodos para estabilizar coelenterazina e análogos e derivados da mesma
JP2022509200A (ja) 2018-11-28 2022-01-20 プロメガ コーポレイション 反応性ペプチド標識付け
US20200200765A1 (en) 2018-12-04 2020-06-25 Promega Corporation Broad spectrum gpcr binding agents
WO2020191339A1 (en) 2019-03-20 2020-09-24 Promega Corporation Photoaffinity probes
SG11202110773RA (en) * 2019-04-10 2021-10-28 Promega Corp Compositions and methods for analyte detection using bioluminescence
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
CN114450326A (zh) 2019-06-24 2022-05-06 普罗美加公司 用于将生物分子递送到细胞中的改性聚胺聚合物
DE102019127894B4 (de) * 2019-10-16 2022-05-12 Sensor-Instruments Entwicklungs- Und Vertriebs-Gmbh Produktkennzeichnungssystem und verfahren zur kennzeichnung eines produkts
US20230031446A1 (en) * 2019-11-22 2023-02-02 The Regents Of The University Of California Split-enzyme system to detect specific dna in living cells
JP2023503653A (ja) 2019-11-27 2023-01-31 プロメガ コーポレイション 多分子ルシフェラーゼペプチド及びポリペプチド
US20210190789A1 (en) 2019-12-10 2021-06-24 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
US20230211023A1 (en) 2020-03-25 2023-07-06 Erasmus University Medical Center Rotterdam Reporter system for radionuclide imaging
US20230296621A1 (en) * 2020-05-11 2023-09-21 Chan Zuckerberg Biohub, Inc. Detection assay for anti-sars-cov-2 antibodies
WO2021237118A2 (en) 2020-05-22 2021-11-25 Promega Corporation Enhancement of kinase target engagement
JP2023540934A (ja) 2020-08-28 2023-09-27 プロメガ コーポレイション Rasタンパク質のターゲットエンゲージメントアッセイ
WO2022245922A1 (en) * 2021-05-18 2022-11-24 The Board Of Regents Of The University Of Texas System Poly-adp ribose (par) tracker optimized split-protein reassembly par detection reagents
WO2023215514A2 (en) 2022-05-04 2023-11-09 Promega Corporation Bioluminescence-triggered photocatalytic labeling
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
US20240132859A1 (en) 2022-05-04 2024-04-25 Promega Corporation Modified dehalogenase with extended surface loop regions
US20240060059A1 (en) 2022-05-04 2024-02-22 Promega Corporation Circularly permuted dehalogenase variants

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1156103A2 (en) * 2000-04-26 2001-11-21 Chisso Corporation Oplophorus luciferase
US20120117667A1 (en) * 2010-11-02 2012-05-10 Klaubert Dieter H Novel coelenterazine substrates and methods of use
CN102459579A (zh) * 2009-05-01 2012-05-16 普罗梅加公司 具有增强的光输出的合成的刺虾萤光素酶

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5914095A (en) 1989-04-07 1999-06-22 Salutar, Inc. Polychelants containg amide bonds
US5756688A (en) 1992-10-14 1998-05-26 Sterling Winthrop Inc. MR imaging compositions and methods
WO1994009056A1 (en) 1992-10-14 1994-04-28 Sterling Winthrop Inc. Chelating polymers
GB9503969D0 (en) 1995-02-28 1995-04-19 Sams Bernard Incrementing mechanism
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US5879739A (en) 1997-02-20 1999-03-09 Tower Semiconductor Ltd. Batch process for forming metal plugs in a dielectric layer of a semiconductor wafer
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
US20040181830A1 (en) 2001-05-07 2004-09-16 Kovalic David K. Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
US7008642B1 (en) 2001-02-12 2006-03-07 Advanced Cardiovascular Systems, Inc. Compositions for achieving a therapeutic effect in an anatomical structure and methods of using the same
US7195884B2 (en) 2002-07-19 2007-03-27 Promega Corp. Methods and kits for transferases
WO2004029284A2 (en) * 2002-09-30 2004-04-08 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
US7237811B1 (en) * 2005-04-20 2007-07-03 Lawrence Barry G Casement window latch assembly
US20090149338A1 (en) 2005-09-30 2009-06-11 Hughes Thomas E System for detecting protein-protein interactions
US7601517B2 (en) 2006-01-10 2009-10-13 Stanford University Split protein self complementing fragments, systems, and methods of use thereof
WO2007132461A2 (en) 2006-05-11 2007-11-22 Ramot At Tel Aviv University Ltd. Classification of protein sequences and uses of classified proteins
US20090075313A1 (en) 2007-05-04 2009-03-19 Stanford University Split protein fragments, split protein systems, methods of making split protein systems, and methods of using split protein systems
CA2704226A1 (en) * 2007-11-01 2009-05-07 The Arizona Board Of Regents On Behalf Of The University Of Arizona Cell-free methods for detecting protein-ligand interctions
EP2222328A4 (en) * 2007-11-15 2013-06-05 Endocyte Inc PROCESS FOR THE ADMINISTRATION OF CONJUGATES
US8546568B2 (en) 2009-02-09 2013-10-01 Jnc Corporation Coelenterazine analogs and manufacturing method thereof
JPWO2010119721A1 (ja) 2009-04-17 2012-10-22 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
US20150125438A1 (en) * 2012-07-20 2015-05-07 Sang Jae Kim Anti-Inflammatory Peptides and Composition Comprising the Same
SG11201504525VA (en) 2012-12-12 2015-07-30 Promega Corp Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
US9797889B2 (en) 2013-03-15 2017-10-24 Promega Corporation Activation of bioluminescence by structural complementation
WO2016040835A1 (en) * 2014-09-12 2016-03-17 Promega Corporation Internal protein tags

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1156103A2 (en) * 2000-04-26 2001-11-21 Chisso Corporation Oplophorus luciferase
CN102459579A (zh) * 2009-05-01 2012-05-16 普罗梅加公司 具有增强的光输出的合成的刺虾萤光素酶
US20120117667A1 (en) * 2010-11-02 2012-05-10 Klaubert Dieter H Novel coelenterazine substrates and methods of use
US20120174242A1 (en) * 2010-11-02 2012-07-05 Brock Binkowski Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SATOSHI INOUYE等: "Secretional luciferase of the luminous shrimp Oplophorus gracilirostris:cDNA cloning of a novel imidazopyrazinone luciferase", 《FEBS LETTERS》 *

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Publication number Publication date
CN117024548A (zh) 2023-11-10
US20160282340A1 (en) 2016-09-29
JP2020097597A (ja) 2020-06-25
KR20150129721A (ko) 2015-11-20
EP2970412B1 (en) 2022-08-10
AU2018256548B2 (en) 2020-05-14
US9869670B2 (en) 2018-01-16
EP3783011A1 (en) 2021-02-24
JP6654557B2 (ja) 2020-02-26
AU2014236949C1 (en) 2018-11-29
US10288605B2 (en) 2019-05-14
US9797890B2 (en) 2017-10-24
AU2018256548A1 (en) 2018-11-22
US20180313825A1 (en) 2018-11-01
US10107800B2 (en) 2018-10-23
KR20230079494A (ko) 2023-06-07
JP2016519065A (ja) 2016-06-30
US20180196038A1 (en) 2018-07-12
AU2020213288A1 (en) 2020-08-27
JP7280842B2 (ja) 2023-05-24
PT2970412T (pt) 2022-09-13
US20180095074A1 (en) 2018-04-05
IL240985A0 (en) 2015-11-30
IL240985B (en) 2020-05-31
EP2970412A1 (en) 2016-01-20
SG10201601929YA (en) 2016-04-28
AU2020213288B2 (en) 2022-07-07
PT3783011T (pt) 2022-10-26
EP4177261A1 (en) 2023-05-10
US20140348747A1 (en) 2014-11-27
JP2023055734A (ja) 2023-04-18
BR112015023394A2 (pt) 2017-11-28
US10184936B2 (en) 2019-01-22
US10648971B2 (en) 2020-05-12
BR112015023394B1 (pt) 2023-08-29
EP4169935A1 (en) 2023-04-26
AU2014236949B2 (en) 2018-08-02
US11493504B2 (en) 2022-11-08
EP2970412A4 (en) 2016-07-27
CA2906063A1 (en) 2014-09-25
SG11201507306VA (en) 2015-10-29
IL273989A (en) 2020-05-31
US20140363375A1 (en) 2014-12-11
PL2970412T3 (pl) 2022-11-14
IL286816B (en) 2022-09-01
EP3783011B1 (en) 2022-09-21
DK3783011T3 (da) 2022-10-17
PL3783011T3 (pl) 2022-11-21
DK2970412T3 (da) 2022-08-29
AU2014236949A1 (en) 2015-09-24
US20200292534A1 (en) 2020-09-17
BR112015023394B8 (pt) 2023-09-26
IL273989B (en) 2021-10-31
US20180306782A1 (en) 2018-10-25
ES2929362T3 (es) 2022-11-28
WO2014151736A1 (en) 2014-09-25
KR20210036422A (ko) 2021-04-02
ES2926463T3 (es) 2022-10-26
IL286816A (en) 2021-10-31
US9797889B2 (en) 2017-10-24

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