CN105123808A - Botanical fungicide for preventing and controlling southern blight of atractylodes and preparation method thereof - Google Patents
Botanical fungicide for preventing and controlling southern blight of atractylodes and preparation method thereof Download PDFInfo
- Publication number
- CN105123808A CN105123808A CN201510645923.7A CN201510645923A CN105123808A CN 105123808 A CN105123808 A CN 105123808A CN 201510645923 A CN201510645923 A CN 201510645923A CN 105123808 A CN105123808 A CN 105123808A
- Authority
- CN
- China
- Prior art keywords
- preparation
- southern blight
- extract
- botanical fungicide
- bighead atractylodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a botanical fungicide for preventing and controlling southern blight of atractylodes and a preparation method thereof. The botanical fungicide is prepared from a straw extracting solution, a semen pharbitidis extracting solution and an exhausted tea extracting solution, wherein the straw extracting solution is obtained by mixing straws and 1% of NaOH aqueous solution, performing microwave processing on the mixed solution at the temperature of 75 DEG C for 3 hours and then performing extraction at the temperature of 75-85 DEG C for 2-3 hours, the semen pharbitidis extracting solution is obtained by mixing semen pharbitidis and 30% of ethanol water solution, performing microwave processing on the mixed solution at the temperature of 50 DEG C for 1-2 hours and then performing extraction at the temperature of 55-60 DEG C for 15-30 minutes, and the exhausted tea extracting solution is obtained by mixing exhausted tea and 70% of ethanol water solution and performing microwave extraction on the mixed solution at the constant temperature of 60 DEG C for 30-45 minutes. The botanical fungicide prepared by means of the preparation method has very good inhabitation effect on the southern blight, contains natural components, is free of pesticide residues after being sprayed, is safe and is environmentally friendly.
Description
Technical field
The present invention relates to the prevention and control field of bighead atractylodes rhizome southern blight, particularly relate to and a kind ofly prevent and treat botanical fungicide of bighead atractylodes rhizome southern blight and preparation method thereof.
Technical background
The bighead atractylodes rhizome is composite family herbaceos perennial, is a kind of traditional Chinese medicine of Chinese medicine, have the title of " southern ginseng ", is also one of " eight Zhe's " well-known at home and abroad.The bighead atractylodes rhizome is used as medicine with rhizome, and having the effects such as strengthening the spleen and replenishing qi, eliminating dampness Li Shui, hidroschesis, is the parts of generic medicinal plants of Chinese patent drug.Zhejiang Province's bighead atractylodes rhizome cultivation history is long, and long-term cultivated area reaches hundreds of hectare, and the bighead atractylodes rhizome of production is high with its active constituent content, quality good, processing is fastidious and marketing domestic and international.But bighead atractylodes rhizome continuous cropping obstacle is serious, and wherein southern blight and root rot are the topmost continuous cropping obstacle factors affecting bighead atractylodes rhizome yield and quality, it is reported, southern blight and the long-term incidence of disease of root rot reach 20%-30%, and the second crop soil incidence of disease can reach more than 50-60%.
Southern blight generally betides high-temperature high humidity season, it is a kind of fungal infectious disease, also be the destructive disease of the bighead atractylodes rhizome, once occur, spread very rapid, can be all dead within a couple of days if prevent not in time, cardinal symptom for from root from rot inwards, rotten place adularescent mycelia or pili, sclerotium is the brown particles of vegetable seed size.Existing prevention and controls is: with gramineous crop crop rotation, select healthy seedling, with pcnb 15kg/hm2 process soil, lesion 50% thiophanate methyl or 50% carbendazim, 1000 times of liquid are watered with before the 1000 times of solution seed soaking plantations of 50% Tuzet before sowing, or sterilize with powder of carbendazim 1000 times of immersion roots of 50%; Field finds that diseased plant is pulled out in time, and band soil mud is concentrated and burnt with going out the bighead atractylodes rhizome, sick cave lime disinfection, and the bordeaux mixture of full field 1:1:120 sprays, and sprays once every 10 days later.The vertical withered clean 100g of mu is watered 50kg spraying.The their early stage mu rotten clever 100g of taro is watered 50kg spraying.Existing prevention and controls need be sprayed insecticide mostly, substantially increase the possibility that residue of pesticide occur in bighead atractylodes rhizome rhizome, and soil fertility can be weakened year by year, although bactericidal effect is obvious in a short time, but Financial cost is high, Pesticide use is frequent, be unfavorable for the sustainability plantation of the bighead atractylodes rhizome.
Summary of the invention
In order to solve the problems of the technologies described above, provide a kind of composition natural and to sclerotium rolfsii sterilization, botanical fungicide that inhibition is good, the invention provides following technical scheme:
Prevent and treat a botanical fungicide for bighead atractylodes rhizome southern blight, form by stalk extract 10-20 part of mass fraction, kaladana's extract 30-50 part, useless tea grounds extract 20-30 part.Containing xylo-oligosaccharide composition in stalk extract, be 35%-40% to the inhibiting rate of sclerotium rolfsii mycelia, containing caffeic acid and NSC 619661 in described kaladana's extract, wherein caffeic acid has bacteriostasis widely, be conducive to the control of multiple bighead atractylodes rhizome germ, and NSC 619661 has strong inhibitory action for sclerotium rolfsii, the generation of sclerotium can be made to be zero substantially, thus reach good southern blight control efficiency, containing catechin in described useless tea grounds extract, there is the effect of antifungal.All containing the composition that can suppress and kill White Army germ in above-mentioned three kinds of materials, combined by these three kinds of materials, effect is not only simple superposition, but can play the effect that complementation helps each other, thus greatly improves the efficiency killing sclerotium rolfsii.
Further, actual when using, the water adding 10-15 times of quality dilutes, and excessive concentration easily causes bighead atractylodes rhizome generation poisoning to burn the problems such as seedling, and concentration is too low, cannot play best bactericidal effect.
Further, the surfactant that mass fraction is 20-30 part is also comprised.Add surfactant and greatly can improve bactericidal effect, be because surfactant can make drug effect rapid osmotic, reduce the evaporation Sum decomposition of body surface medicament, reduce stop in body, deaden and degraded, thus the active ingredient entering target is increased, improve drug effect.
Further, described surfactant is the one in sorbitan fatty acid ester, sucrose fatty ester.Although these natural surfactant surface-actives are less, nontoxic, biological degradability is good, and consumption is suitable for also playing the effect improving drug effect.
Prevent and treat a preparation method for the botanical fungicide of bighead atractylodes rhizome southern blight, comprise the following steps:
The preparation of S1, stalk extract: after stalk drying and crushing, cross 40 mesh sieves, add the NaOH aqueous solution of 1%, liquid ratio is 10:1, under the power of 400w, 75 DEG C of microwave treatment 3h, then at 75-85 DEG C of lixiviate 2-3h, remove residue and get supernatant; In microwave digestion process, leaching liquor kind, leaching liquor concentration, microwave power, temperature, extraction time all produce larger impact to the recovery rate of microwave digestion, adopt the recovery rate of xylo-oligosaccharide in the condition stalk of step S1 to reach more than 60-80%.
The preparation of S2, kaladana's extract: after kaladana's drying and crushing, crosses 50 mesh sieves, adds the ethanol water of 25%, liquid ratio is 18:1, under the power of 200w, and 50 DEG C of microwave treatment 1-2h, again at 55-60 DEG C of lixiviate 15-30min, remove residue and get supernatant; The recovery rate of the NSC 619661 of the process conditions kaladana in step S2 is adopted to reach more than 65%.
The preparation of S3, useless tea grounds extract: tea grounds crushed after being dried of giving up, cross 50 mesh sieves, add the ethanol water of 70%, liquid ratio is 12:1, and under the power of 400w, 60 DEG C of constant temperature microwave digestion 30-45min, remove residue and get supernatant; The catechin recovery rate adopting the process conditions in step S3 to give up in tea grounds can reach more than 75%.
S4,3 kinds of leaching liquors prepared by step S1-S3 mixing and stirring in proportion at normal temperatures, In Shade for subsequent use.
Beneficial effect of the present invention is:
(1), in raw material major part adopts stalk, useless tea grounds etc., and not only cost is low, also solves the problem of waste pollution and the wasting of resources simultaneously.
(2), bactericide preparation process adopts microwave digestion method, the simple and non-environmental-pollution of technical process.
(3), can effectively prevent and treat bighead atractylodes rhizome southern blight, bactericidal effect is good, and is more suitable for the primary prevention of southern blight.
(4), microbiocidal composition is natural, uses rear non agricultural chemical residuum risk, and the bighead atractylodes rhizome active constituent content that the later stage produces is high.
Embodiment
Prevent and treat a botanical fungicide for bighead atractylodes rhizome southern blight, form by the stalk extract 20 parts of mass fraction, kaladana's extract 40 parts, useless tea grounds extract 25 parts, sucrose fatty ester 25 parts.
Containing xylo-oligosaccharide composition in stalk extract, be 35%-40% to the inhibiting rate of sclerotium rolfsii mycelia, containing caffeic acid and NSC 619661 in described kaladana's extract, wherein caffeic acid has bacteriostasis widely, be conducive to the control of multiple bighead atractylodes rhizome germ, and NSC 619661 has strong inhibitory action for sclerotium rolfsii, the generation of sclerotium can be made to be zero substantially, thus reach good southern blight control efficiency, containing catechin in described useless tea grounds extract, there is the effect of antifungal.All containing the composition that can suppress and kill White Army germ in above-mentioned three kinds of materials, combined by these three kinds of materials, effect is not only simple superposition, but can play the effect that complementation helps each other, thus greatly improves the efficiency killing sclerotium rolfsii.Add surfactant and greatly can improve bactericidal effect, be because surfactant can make drug effect rapid osmotic, reduce the evaporation Sum decomposition of body surface medicament, reduce stop in body, deaden and degraded, thus the active ingredient entering target is increased, improve drug effect.Although sucrose fatty ester surface-active is less, nontoxic, biological degradability good.
Prevent and treat a preparation method for the botanical fungicide of bighead atractylodes rhizome southern blight, comprise the following steps:
The preparation of S1, stalk extract: after stalk drying and crushing, cross 40 mesh sieves, add the NaOH aqueous solution of 1%, liquid ratio is 10:1, under the power of 400w, 75 DEG C of microwave treatment 3h, then at 80 DEG C of lixiviate 2h, remove residue and get supernatant; In microwave digestion process, leaching liquor kind, leaching liquor concentration, microwave power, temperature, extraction time all produce larger impact to the recovery rate of microwave digestion, and the recovery rate adopting xylo-oligosaccharide in the condition stalk of step S1 is about 70%.
The preparation of S2, kaladana's extract: after kaladana's drying and crushing, crosses 50 mesh sieves, adds the ethanol water of 25%, liquid ratio is 18:1, under the power of 200w, and 50 DEG C of microwave treatment 1-2h, again at 55-60 DEG C of lixiviate 15-30min, remove residue and get supernatant; The recovery rate adopting the NSC 619661 of the process conditions kaladana in step S2 is about 65%.
The preparation of S3, useless tea grounds extract: tea grounds crushed after being dried of giving up, cross 50 mesh sieves, add the ethanol water of 70%, liquid ratio is 12:1, and under the power of 400w, 60 DEG C of constant temperature microwave digestion 30-45min, remove residue and get supernatant; The process conditions catechin recovery rate given up in tea grounds in step S3 is adopted to be about 75%.
S4,3 kinds of leaching liquors prepared by step S1-S3 mixing and stirring in proportion at normal temperatures, In Shade for subsequent use.
The bactericide adopting technical method of the present invention to prepare reaches 87.5% for the inhibiting rate of bighead atractylodes rhizome sclerotium rolfsii, wherein, inhibiting rate (%)=[(control group bacterium colony average diameter-pastille group bacterium colony average diameter)/control group bacterium colony expansion average diameter] * 100%.
Actual when using, the water adding 10 times of quality dilutes, and namely starts to spray after the bighead atractylodes rhizome is sowed, and every 5-7 days once, high-temperature high humidity season or area can be sprayed once every 2-3 days.
With above-mentioned foundation desirable embodiment of the present invention for enlightenment, by above-mentioned description, relevant staff in the scope not departing from this invention technological thought, can carry out various change and amendment completely.The technical scope of this invention is not limited to the content on specification, must determine its technical scope according to right.
Claims (5)
1. prevent and treat a botanical fungicide for bighead atractylodes rhizome southern blight, it is characterized in that, form by stalk extract 10-20 part of mass fraction, kaladana's extract 30-50 part, useless tea grounds extract 20-30 part.
2. a kind of botanical fungicide preventing and treating bighead atractylodes rhizome southern blight as claimed in claim 1, is characterized in that, actual when using, the water adding 10-15 times of quality dilutes.
3. a kind of botanical fungicide preventing and treating bighead atractylodes rhizome southern blight as claimed in claim 1, is characterized in that, also comprises the surfactant that mass fraction is 20-30 part.
4. a kind of botanical fungicide preventing and treating bighead atractylodes rhizome southern blight as claimed in claim 3, is characterized in that, described surfactant is the one in sorbitan fatty acid ester, sucrose fatty ester.
5. prevent and treat a preparation method for the botanical fungicide of bighead atractylodes rhizome southern blight, it is characterized in that, comprise the following steps:
The preparation of S1, stalk extract: after stalk drying and crushing, cross 40 mesh sieves, add the NaOH aqueous solution of 1%, liquid ratio is 10:1, under the power of 400w, 75 DEG C of microwave treatment 3h, then at 75-85 DEG C of lixiviate 2-3h, remove residue and get supernatant;
The preparation of S2, kaladana's extract: after kaladana's drying and crushing, crosses 50 mesh sieves, adds the ethanol water of 25%, liquid ratio is 18:1, under the power of 200w, and 50 DEG C of microwave treatment 1-2h, again at 55-60 DEG C of lixiviate 15-30min, remove residue and get supernatant;
The preparation of S3, useless tea grounds extract: tea grounds crushed after being dried of giving up, cross 50 mesh sieves, add the ethanol water of 70%, liquid ratio is 12:1, and under the power of 400w, 60 DEG C of constant temperature microwave digestion 30-45min, remove residue and get supernatant;
S4,3 kinds of leaching liquors prepared by step S1-S3 mixing and stirring in proportion at normal temperatures, In Shade for subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510645923.7A CN105123808A (en) | 2015-10-08 | 2015-10-08 | Botanical fungicide for preventing and controlling southern blight of atractylodes and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510645923.7A CN105123808A (en) | 2015-10-08 | 2015-10-08 | Botanical fungicide for preventing and controlling southern blight of atractylodes and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105123808A true CN105123808A (en) | 2015-12-09 |
Family
ID=54709697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510645923.7A Pending CN105123808A (en) | 2015-10-08 | 2015-10-08 | Botanical fungicide for preventing and controlling southern blight of atractylodes and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105123808A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106304898A (en) * | 2016-08-16 | 2017-01-11 | 文县科学技术局 | Prevention and control method for southern blight of walnut seedlings |
| CN108497001A (en) * | 2018-05-04 | 2018-09-07 | 河南省林业科学研究院 | A kind of taking root liquid and preparation method thereof for Fructus Forsythiae cuttage and seedling culture |
| CN112741119A (en) * | 2020-12-28 | 2021-05-04 | 浙江农林大学 | Natural southern blight bactericide based on cinnamomum camphora leaves and preparation method thereof |
| WO2021227300A1 (en) * | 2020-05-11 | 2021-11-18 | 枣庄学院 | Biopesticide for preventing and controlling downy mildew |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515578A (en) * | 2003-01-10 | 2004-07-28 | 郑庆义 | Method for extracting xylose and xylitol from straw of crops |
| CN102584907A (en) * | 2012-01-06 | 2012-07-18 | 黑龙江浩源生物科技有限公司 | Method for producing xylose and arabinose with corn straw as raw material |
| CN103340211A (en) * | 2013-07-10 | 2013-10-09 | 丹阳市淸云农业发展有限公司 | Method for preventing southern blight by using white atractylodes rhizome |
| CN104938536A (en) * | 2015-06-04 | 2015-09-30 | 胡凡营 | Plant-derived biopesticide and preparation method thereof |
-
2015
- 2015-10-08 CN CN201510645923.7A patent/CN105123808A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515578A (en) * | 2003-01-10 | 2004-07-28 | 郑庆义 | Method for extracting xylose and xylitol from straw of crops |
| CN102584907A (en) * | 2012-01-06 | 2012-07-18 | 黑龙江浩源生物科技有限公司 | Method for producing xylose and arabinose with corn straw as raw material |
| CN103340211A (en) * | 2013-07-10 | 2013-10-09 | 丹阳市淸云农业发展有限公司 | Method for preventing southern blight by using white atractylodes rhizome |
| CN104938536A (en) * | 2015-06-04 | 2015-09-30 | 胡凡营 | Plant-derived biopesticide and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 万定良 等: "《饮料生产技术与配方》", 30 September 1987, 江西科学技术出版社 * |
| 方丽 等: "部分糖类及化感物质对白术白绢病菌与根腐病菌的影响", 《浙江农业学报》 * |
| 高中良 等: "牵牛子中咖啡酸和咖啡酸乙酯的提取研究", 《安徽农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106304898A (en) * | 2016-08-16 | 2017-01-11 | 文县科学技术局 | Prevention and control method for southern blight of walnut seedlings |
| CN108497001A (en) * | 2018-05-04 | 2018-09-07 | 河南省林业科学研究院 | A kind of taking root liquid and preparation method thereof for Fructus Forsythiae cuttage and seedling culture |
| WO2021227300A1 (en) * | 2020-05-11 | 2021-11-18 | 枣庄学院 | Biopesticide for preventing and controlling downy mildew |
| CN112741119A (en) * | 2020-12-28 | 2021-05-04 | 浙江农林大学 | Natural southern blight bactericide based on cinnamomum camphora leaves and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106588342A (en) | Special leaf fertilizer for mulberry and preparation method of special leaf fertilizer for mulberry | |
| CN104782686A (en) | Peanut Chinese medicinal bactericide | |
| CN103130532A (en) | Preparation method of special base fertilizer for plant honeysuckle | |
| CN104663396A (en) | Planting method for dendrobium officinale | |
| CN105123808A (en) | Botanical fungicide for preventing and controlling southern blight of atractylodes and preparation method thereof | |
| CN105340999A (en) | Chinese herbal medicine biological prevention and control method for Chinese chive maggots | |
| CN101627689B (en) | Control method of gentiana rigescens spot blight | |
| CN105475376A (en) | Natural plant bactericide for controlling dendrobium insect diseases | |
| CN104496668A (en) | Green organic chemical fertilizer and preparation method thereof | |
| CN105028528A (en) | Pesticide for preventing and controlling cotton anthracnose | |
| CN101496529A (en) | Camellia oleifera environment-friendly pesticide | |
| CN106831202A (en) | A kind of potato special fertilizer and its preparation and application process | |
| CN104920514A (en) | Traditional Chinese medicine bactericide for peanuts | |
| CN105410061A (en) | Compound bactericide for preventing and controlling apple tree rot diseases | |
| CN105076257A (en) | Chinese chive maggot preventing and treating method | |
| CN104920508A (en) | Traditional Chinese medicine bactericide for peanuts and preparation method therefor | |
| CN104886184B (en) | A kind of botanical fungicide and preparation method for preventing and treating tobacco mosaic virus disease | |
| CN101843267A (en) | High-efficiency nontoxic insecticide and preparation method thereof | |
| CN105340993A (en) | Fruit tree plant-based pesticide and preparation method | |
| CN104350986A (en) | Method for preventing and treating diseases and insect pests of pawpaw | |
| CN107372653A (en) | Medicament and the application of snail are prevented and treated in officinal dendrobium stem plantation production | |
| CN106857727A (en) | A kind of environment friendly agricultural for preventing and treating tomato trialeurodes vaporariorum | |
| CN105284945A (en) | Method for controlling sugarcane pineapple disease | |
| CN108476825A (en) | A kind of control method of Radix Angelicae Sinensis root rot | |
| CN105248468A (en) | Bactericide for preventing and controlling Red Fuji apple ring spot |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151209 |
|
| WD01 | Invention patent application deemed withdrawn after publication |