CN105112520B - 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒 - Google Patents

原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒 Download PDF

Info

Publication number
CN105112520B
CN105112520B CN201510514913.XA CN201510514913A CN105112520B CN 105112520 B CN105112520 B CN 105112520B CN 201510514913 A CN201510514913 A CN 201510514913A CN 105112520 B CN105112520 B CN 105112520B
Authority
CN
China
Prior art keywords
seq
nucleotide sequence
nucleotide
sequence
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201510514913.XA
Other languages
English (en)
Other versions
CN105112520A (zh
Inventor
M·德博克勒尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience LP
Original Assignee
Bayer CropScience NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer CropScience NV filed Critical Bayer CropScience NV
Publication of CN105112520A publication Critical patent/CN105112520A/zh
Application granted granted Critical
Publication of CN105112520B publication Critical patent/CN105112520B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Extrusion Moulding Of Plastics Or The Like (AREA)
  • Medical Treatment And Welfare Office Work (AREA)

Abstract

本发明提供了能够在生物样品中快速和清楚鉴定原种事件A5547‑127的手段。

Description

原种事件A5547-127以及在生物样品中鉴定此类事件的方法 和试剂盒
本申请是申请日为2006年4月4日的、发明名称为“原种事件A5547-127以及在生物样品中鉴定此类事件的方法和试剂盒”的中国专利申请200680011600.1(PCT/EP2006/003455)的分案申请。
发明领域
本发明涉及鉴定在生物样品中存在包含特定转化事件A5547-127的植物材料，以及包含此类事件的转基因大豆植物、植物材料和种子的方法和试剂盒。本发明的大豆植物将除草剂耐受表型与农艺学特征、遗传稳定性和对不同遗传背景的适应性结合在一起，等同于缺乏杂草压力的非转化大豆品系。
发明背景
在植物中转基因的表型表达依据基因自身结构和其在植物基因组中的位置而决定。同时，转基因(外源DNA)存在于基因组的不同位置将以不同方式影响植物的整体表型。在农艺学或工业上，通过基因操作向植物中成功引入商业上感兴趣的性状是一个依赖于不同因素的漫长过程。遗传转化的植物实际上的转化和再生仅仅是一系列选择步骤的第一步，这一系列步骤包括广泛的遗传特征鉴定、育种和田间试验的评估，最终导致原种事件的选择。
考虑到在新食品/饲料、GMO和非GMO产品的分离以及独有材料的鉴定方面的讨论，对于原种事件的明确鉴定变得日益重要。理想的此类鉴定方法是既迅速又简单、不需要很多的实验室装置。此外，该方法应该提供在没有专家解释的情况下允许明确鉴定原种事件的结果，但是如果需要可以展示给专家详察。
通过用包含编码耐受膦丝菌素的合成pat基因的质粒转化大豆，选择A5547-127作为在大豆(Glycine max L.)发育中对抗除草剂的原种事件，并且可以作为Liberty大豆商业销售。这里描述在生物样品中鉴定原种事件A5547-127的简单和明确的方法中使用的手段。
发明概述
本发明涉及在生物样品中鉴定原种事件A5547-127的方法，其基于特异性识别A5547-127的5’和/或3’侧翼序列的引物或探针。
更具体地，本发明涉及方法，其包含扩增生物样品中存在的核酸序列，即使用至少两种引物进行聚合酶链式反应，其中一种引物识别A5547-127的5’或3’侧翼区，另外一种引物识别外源DNA中的序列，优选获得的DNA片段在100和500bp之间。引物可以分别识别A5547-127的5’侧翼区内的序列(SEQ ID No.1，从1-311位点)或3’侧翼区内的序列(SEQ IDNo.2从510-1880位点的互补序列)和外源DNA内的序列(SEQ ID No 1从312-810位点的互补序列或SEQ ID No 2从1-510位点)。此处所描述的识别5’侧翼区的引物可能包含SEQ IDNo.15的核苷酸序列，且识别外源DNA内序列的引物可能包含SEQ ID No.13的核苷酸序列。
本发明更具体涉及在生物样品中鉴定原种事件A5547-127的方法，其包含扩增生物样品中存在的核酸序列，即使用分别含有SEQ ID No.15和SEQ ID No.13的核苷酸序列的两种引物进行聚合酶链式反应，获得的DNA片段约为151bp。
本发明还涉及此处描述的A5547-127的特异侧翼序列，可以用其发展在生物样品中特异鉴别A5547-127的方法。更具体地，本发明涉及A5547-127的5’和/或3’侧翼区，可以用其发展此处进一步描述的特异引物和探针。本发明还涉及生物样品中存在A5547-127的鉴定方法，其基于使用此类特异引物或探针。引物可以由17到约200个连续的核苷酸序列(选自SEQ ID No 1从1-311核苷酸序列、或SEQ ID 2从510-1880核苷酸的互补核苷酸序列)组成，与由17到约200个连续的核苷酸序列的引物(选自SEQ ID No 1从312-810核苷酸、或SEQ ID No 2从1-510核苷酸的互补序列)组合使用。引物也可以在它们的3’末端包含这些核苷酸序列，并且引物还包含非相关序列或从所述核苷酸序列衍生而来、但是含有错配的序列。
本发明还涉及在生物样品中鉴定原种事件A5547-127的试剂盒，所述试剂盒包括至少一种引物或探针，其特异性识别A5547-127的5’或3’侧翼区。
本发明的试剂盒除包含特异性识别A5547-127的5’或3’侧翼区的引物以外，可能还包含第二种引物用于PCR鉴定方法，所述第二种引物特异性识别A5547-127的外源DNA内的序列。优选地，本发明的试剂盒包含两种特异引物，其中一种识别A5547-127的5’侧翼区内的序列，而其中另一种识别外源DNA内的序列。特别是识别5’侧翼区的引物可能包含SEQID No.14的核苷酸序列，并且识别转基因的引物可能包含SEQ ID No.13的核苷酸序列或任何此处所描述的其它引物。
本发明还涉及在生物样品中鉴定原种事件A5547-127的试剂盒，所述试剂盒包含具有SEQ ID No.13和SEQ ID No.15的核苷酸序列的PCR引物，用于此处所描述的A5547-127PCR鉴定方法。
本发明也涉及在生物样品中鉴定原种事件A5547-127的试剂盒，所述试剂盒包含特异性探针，该探针具有与A5547-127特定区域有80％到100％序列同一性的序列的相应(或互补)序列。优选的探针序列相应于包含部分A5547-127的5’或3’侧翼区的特定区域。更优选的特异性探针具有(或互补于)与SEQ ID No.1的360到460核苷酸或SEQ ID No.2的460到560核苷酸序列有80％到100％序列同一性的序列。
本发明包括的方法和试剂盒可用于不同的目的，例如(但是并不限于)如下所示：在植物、植物材料或产品中鉴定A5547-127的存在或缺失，其中产品是例如(但不限于)包含植物材料的或从植物材料衍生的食物或饲料产品(新鲜的或经处理的)；另外或可选择地，本发明的方法和试剂盒可被用于以分离转基因和非转基因材料为目的的转基因植物材料鉴定；另外或可选择地，本发明的方法和试剂盒可被用于测定包含A5547-127的植物材料的质量(即纯材料的百分数)。
本发明还涉及A5547-127的5’和/或3’侧翼区，以及从A5547-127的5’和/或3’侧翼序列发展而来的特异引物和探针。
本发明也涉及包含原种事件A5547-127的大豆植物、其部分、细胞、种子和子代植物。此类植物、其部分、细胞、种子和子代植物可以使用此处所描述的方法来鉴定。
发明详述
通常由细胞或组织的转化(或通过另外的基因操作)产生重组DNA分子掺入到植物基因组中。掺入的特定位置是由于“随机”整合决定或在预定位置(如果使用靶向整合方法)。
通过用重组DNA或“转化DNA”转化植物细胞或组织而导致引入DNA到植物基因组，且来源于此类转化DNA的DNA在下文被称为“外源DNA”，其含有一个或多个“转基因”。在本发明中，“植物DNA”指来源于被转化植物的DNA。植物DNA通常被发现位于相应野生型植物相同的遗传位点。外源DNA的特征在于重组DNA分子在植物基因组中掺入位点的位置和构象。重组DNA插入植物基因组的位点也被称为“插入位点”或“靶位点”。重组DNA插入植物基因组中可以与植物DNA的缺失联系在一起，称为“靶位缺失”。此处所使用的“侧翼区”或“侧翼序列”指至少20bp，优选至少50bp，和直到5000bp的植物基因组序列，其位于外源DNA邻近上游、或邻近下游，并且都与外源DNA邻接。转化过程导致外源DNA随机整合，从而产生带有不同侧翼区的转化体，所述侧翼区对于每种转化体是特征性的且独特的。当重组DNA通过传统杂交被引入植物中时，其在植物基因组中的插入位点、或其侧翼区通常不会被改变。此处所使用的“插入区”指相应于至少40bp，优选至少100bp，和直到10000bp的区域，包含植物基因组中外源DNA的上游和/或下游侧翼区的序列。考虑到由于物种内突变造成的微小差别，与同种植物杂交时，插入区将保持与包含原来从转化获得的植物中外源DNA上游和下游侧翼区序列具有至少85％、优选90％、更优选95％和最优选100％的序列同一性。
事件定义为(人工的)遗传位点，其由于基因工程而携带包含至少一个拷贝目的基因的转基因。事件典型的等位基因状态为存在或缺失外源DNA。事件的表型特征在于转基因的表达。在遗传水平上，事件是植物遗传组成的部分。在分子水平上，事件的特征在于限制性酶切图谱(例如通过Southern印迹确定)、转基因的上游和/或下游侧翼序列、转基因的分子标记的位置和/或分子构象。通常用包含至少一个目的基因的转化DNA转化植物会导致多重事件，其中每个事件都是唯一的。
此处所使用的原种事件是选自一组事件中的事件，其通过用相同的转化DNA转化、或通过用此种转化所获得的植物回交而获得，基于转基因的表达和稳定性、及其与包含它的植物的最适农艺学特性的相容性。因此原种事件的筛选标准为一个或多个、优选两个或多个、有利地包含所有如下标准：
a)外源DNA的存在不会危害植物的其它所希望的特性，例如那些有关农业操作或商业价值的特性；
b)事件的特征在于良好的确定的分子构象，其可以稳定遗传并且可以对其发展作为鉴定对照的适当手段；
c)在事件为杂合子(或半合子)和纯合子条件下，在携带事件的植物可能暴露于正常农业使用的环境条件范围内以商业上可接受的水平，目的基因展示正确的、适当和稳定的时空表型表达。
优选的外源DNA与植物基因组中的位置相关联，该位置允许基因容易的渐渗到希望的商业遗传背景中。
事件作为原种事件状态的确认是通过原种事件渐渗到不同相关的遗传背景中，并且观察其依从例如上述a)、b)和c)中一个、两个或全部的标准。
因此“原种事件”指包含外源DNA的遗传位点，其符合上述标准。植物、植物材料或子代例如种子，在其基因组中可以包含一个或多个原种事件。
用于鉴定原种事件或包含原种事件的植物、植物材料、或含有植物材料的产品的方法基于原种事件的特定基因组特性，例如包含外源DNA的基因组区域的特异限制性酶切图谱、分子标记或外源DNA侧翼区序列。
一旦外源DNA的一个或两个侧翼区被测序，就可经由分子生物学技术来发展特异性识别样品中核酸(DNA或RNA)的这个(这些)序列的引物和探针。例如PCR方法可以用于鉴定生物样品中(例如植物、植物材料或包含植物材料的产品的样品)的原种事件。此类PCR基于至少两种特异性“引物”，一种识别原种事件的5’或3’侧翼区内的序列，另外一种识别外源DNA内的序列。引物优选具有15-35个核苷酸的序列，其在最适PCR条件下分别“特异性识别”原种事件的5’或3’侧翼区内的序列和原种事件的外源DNA，以便从包含原种事件的核酸样品中扩增出特异性片段(“整合片段”或识别的扩增子)。这表示在最佳PCR条件下扩增的只有定向整合片段，而没有植物基因组或外源DNA的其它序列。
适合本发明的PCR引物可能为如下：
-长度在17nt到约300nt范围的寡核苷酸，在它们的3’末端包含选自5’侧翼序列(SEQ ID No 1从1到311核苷酸)的至少17个连续的核苷酸，优选20个连续的核苷酸(识别5’侧翼序列的引物)；或者
-长度在17nt到约300nt范围的寡核苷酸，在它们的3’末端包含选自3’侧翼序列(SEQ ID No 2从510到1880核苷酸的互补序列)的至少17个连续的核苷酸，优选20个连续的核苷酸(识别3’侧翼序列的引物)；或者
-长度在17nt到约510nt范围的寡核苷酸，在它们的3’末端包含选自插入DNA序列(SEQ ID No 1从312到810核苷酸的互补序列)的至少17个连续的核苷酸，优选20个连续的核苷酸(识别外源DNA的引物)；或者
-长度在17nt到约300nt范围的寡核苷酸，包含选自插入DNA序列(SEQ ID No 2从1到509核苷酸)的至少17个连续的核苷酸，优选20个核苷酸。
引物当然可以比所述的17个连续核苷酸更长，长度可以是例如20、21、30、35、50、75、100、150、200nt或更长。引物可以完全由选自所述侧翼序列和外源DNA序列的核苷酸序列组成。然而，引物5’末端(即在位于3’端的17个连续核苷酸之外)的核苷酸序列并不是决定性的。因此，引物的5’序列可以由选自侧翼序列或外源DNA的核苷酸序列组成，但是适当的可以含有一些错配(例如1、2、5、10个错配)。引物的5’序列甚至可以完全包括与侧翼序列或外源DNA无关的核苷酸序列，例如代表限制性内切酶识别位点的核苷酸序列。此类无关的序列或带有错配的侧翼DNA序列应该优选长度不超过100个核苷酸，更优选不超过50个或甚至25个核苷酸。
而且，假如所述位于3’端的17个连续核苷酸并非从外源DNA或SEQ ID No 1或2中由植物衍生序列唯一衍生而来的，那么合适的引物可能包含或由在其3’末端跨植物DNA衍生序列和外源DNA序列(位于SEQ ID No 1的311-312核苷酸和SEQ ID No 2的509-510核苷酸)之间连接区的核苷酸序列组成。
因此，适合本发明的PCR引物也可能如下：
-长度在17nt到约300nt范围的寡核苷酸，在它们的3’末端包含选自SEQ ID No 1从1到325核苷酸的至少17个连续的核苷酸序列，优选20个连续的核苷酸序列；或
-长度在17nt到约300nt范围的寡核苷酸，在它们的3’末端包含选自SEQ ID No 2从495到1880核苷酸的互补序列的至少17个连续的核苷酸序列，优选20个连续的核苷酸序列；或
-长度在17nt到约300nt范围的寡核苷酸，在它们的3’末端包含选自SEQ ID No 1从295到810核苷酸的互补序列的至少17个连续的核苷酸序列，优选20个连续的核苷酸序列；或
-长度在17nt到约300nt范围的寡核苷酸，包含选自SEQ ID No 2从1到525核苷酸的至少17个连续的核苷酸序列，优选20个连续的核苷酸序列。
熟练的技术人员也会立刻清楚所选的适当PCR引物对也应该不包含彼此互补的序列。
出于本发明的目的，“SEQ ID No:X代表的核苷酸序列的互补序列”所指的核苷酸是根据Chargaff规则通过用其互补的核苷酸取代，并以5’到3’方向(即以所代表的核苷酸序列相反的方向)读码，从所代表的核苷酸序列衍生而来的核苷酸序列。
适当的引物的实例是SEQ ID No 3、SEQ ID No 4、SEQ ID No 5(识别5'侧翼序列的引物)、SEQ ID No 6、SEQ ID No 7、SEQ ID No 8、SEQ ID No 9、SEQ ID No 10(识别外源DNA的引物，与识别5'侧翼序列的引物一同使用)、SEQ ID No 11、SEQ ID No 12、SEQ ID No13、SEQ ID No14(识别外源DNA的引物，与识别3'侧翼序列的引物一同使用)、SEQ ID No15、SEQ ID No 16或SEQ ID No 17(识别3'侧翼序列的引物)的寡核苷酸序列。
其它适当的寡核苷酸引物的实例在其3’末端包含如下序列或由此类序列组成：
a.识别5'侧翼序列的引物:
-SEQ ID No 1从45到64的核苷酸序列
-SEQ ID No 1从22到41的核苷酸序列
-SEQ ID No 1从47到64的核苷酸序列
-SEQ ID No 1从183到202的核苷酸序列
-SEQ ID No 1从184到203的核苷酸序列
-SEQ ID No 1从301到320的核苷酸序列
-SEQ ID No 1从303到322的核苷酸序列
-SEQ ID No 1从306到325的核苷酸序列
-SEQ ID No 1从36到55的核苷酸序列
-SEQ ID No 1从182到202的核苷酸序列
-SEQ ID No 1从183到203的核苷酸序列
-SEQ ID No 1从184到202的核苷酸序列
-SEQ ID No 1从185到203的核苷酸序列
-SEQ ID No 1从185到204的核苷酸序列
-SEQ ID No 1从292到311的核苷酸序列
-SEQ ID No 1从295到314的核苷酸序列
-SEQ ID No 1从307到325的核苷酸序列
-SEQ ID No 1从8到27的核苷酸序列
-SEQ ID No 1从10到29的核苷酸序列
-SEQ ID No 1从11到30的核苷酸序列
-SEQ ID No 1从13到32的核苷酸序列
-SEQ ID No 1从20到41的核苷酸序列
-SEQ ID No 1从35到54的核苷酸序列
-SEQ ID No 1从37到55的核苷酸序列
-SEQ ID No 1从66到85的核苷酸序列
-SEQ ID No 1从67到86的核苷酸序列
-SEQ ID No 1从68到87的核苷酸序列
-SEQ ID No 1从181到202的核苷酸序列
-SEQ ID No 1从182到203的核苷酸序列
-SEQ ID No 1从184到204的核苷酸序列
-SEQ ID No 1从185到202的核苷酸序列
-SEQ ID No 1从186到204的核苷酸序列
-SEQ ID No 1从186到203的核苷酸序列
-SEQ ID No 1从248到267的核苷酸序列
-SEQ ID No 1从249到268的核苷酸序列
-SEQ ID No 1从290到309的核苷酸序列
-SEQ ID No 1从291到311的核苷酸序列
-SEQ ID No 1从293到311的核苷酸序列
-SEQ ID No 1从294到314的核苷酸序列
-SEQ ID No 1从301到322的核苷酸序列
-SEQ ID No 1从303到320的核苷酸序列
-SEQ ID No 1从305到322的核苷酸序列
-SEQ ID No 1从308到325的核苷酸序列
-SEQ ID No 1从11到29的核苷酸序列
-SEQ ID No 1从36到54的核苷酸序列
-SEQ ID No 1从41到61的核苷酸序列
-SEQ ID No 1从43到64的核苷酸序列
-SEQ ID No 1从66到86的核苷酸序列
-SEQ ID No 1从67到85的核苷酸序列
-SEQ ID No 1从67到87的核苷酸序列
-SEQ TD No 1从68到86的核苷酸序列
-SEQ ID No 1从69到87的核苷酸序列
-SEQ ID No 1从180到197的核苷酸序列
-SEQ ID No 1从183到204的核苷酸序列
-SEQ ID No 1从187到204的核苷酸序列
-SEQ ID No 1从200到219的核苷酸序列
-SEQ ID No 1从246到263的核苷酸序列
-SEQ ID No 1从247到267的核苷酸序列
-SEQ ID No 1从248到268的核苷酸序列
-SEQ ID No 1从249到267的核苷酸序列
-SEQ ID No 1从250到268的核苷酸序列
-SEQ ID No 1从290到311的核苷酸序列
-SEQ ID No 1从291到308的核苷酸序列
-SEQ ID No 1从291到309的核苷酸序列
-SEQ ID No 1从293到214的核苷酸序列
-SEQ ID No 1从8到29的核苷酸序列
-SEQ ID No 1从11到32的核苷酸序列
-SEQ ID No 1从37到54的核苷酸序列
-SEQ ID No 1从40到61的核苷酸序列
-SEQ ID No 1从64到85的核苷酸序列
-SEQ ID No 1从65到86的核苷酸序列
-SEQ ID No 1从66到87的核苷酸序列
-SEQ ID No 1从68到85的核苷酸序列
-SEQ ID No 1从69到86的核苷酸序列
-SEQ ID No 1从197到218的核苷酸序列
-SEQ ID No 1从201到219的核苷酸序列
-SEQ ID No 1从201到218的核苷酸序列
-SEQ ID No 1从234到253的核苷酸序列
-SEQ ID No 1从244到263的核苷酸序列
-SEQ ID No 1从246到267的核苷酸序列
-SEQ ID No 1从247到268的核苷酸序列
-SEQ ID No 1从250到267的核苷酸序列
-SEQ ID No 1从292到309的核苷酸序列
-SEQ ID No 1从198到219的核苷酸序列
-SEQ ID No 1从202到219的核苷酸序列
-SEQ ID No 1从233到253的核苷酸序列
-SEQ ID No 1从235到254的核苷酸序列
-SEQ ID No 1从235到253的核苷酸序列
-SEQ ID No 1从243到263的核苷酸序列
-SEQ ID No 1从232到253的核苷酸序列
-SEQ ID No 1从234到254的核苷酸序列
-SEQ ID No 1从242到263的核苷酸序列
-SEQ ID No 1从233到254的核苷酸序列
-SEQ ID No 1从234到255的核苷酸序列
-SEQ ID No 1从294到215的核苷酸序列
-SEQ ID No 1从247到266的核苷酸序列
-SEQ ID No 1从248到266的核苷酸序列
-SEQ ID No 1从249到266的核苷酸序列
b.识别外源DNA序列的引物，与识别5'侧翼序列的引物一同使用:
-SEQ ID No 1从781到800核苷酸的互补核苷酸序列
-SEQ ID No 1从301到320核苷酸的互补核苷酸序列
-SEQ ID No 1从303到322核苷酸的互补核苷酸序列
-SEQ ID No 1从442到461核苷酸的互补核苷酸序列
-SEQ ID No 1从444到463核苷酸的互补核苷酸序列
-SEQ ID No 1从778到797核苷酸的互补核苷酸序列
-SEQ ID No 1从781到799核苷酸的互补核苷酸序列
-SEQ ID No 1从788到807核苷酸的互补核苷酸序列
-SEQ ID No 1从318到337核苷酸的互补核苷酸序列
-SEQ ID No 1从322到341核苷酸的互补核苷酸序列
-SEQ ID No 1从325到344核苷酸的互补核苷酸序列
-SEQ ID No 1从329到348核苷酸的互补核苷酸序列
-SEQ ID No 1从353到372核苷酸的互补核苷酸序列
-SEQ ID No 1从376到395核苷酸的互补核苷酸序列
-SEQ ID No 1从378到397核苷酸的互补核苷酸序列
-SEQ ID No 1从384到403核苷酸的互补核苷酸序列
-SEQ ID No 1从440到459核苷酸的互补核苷酸序列
-SEQ ID No 1从442到460核苷酸的互补核苷酸序列
-SEQ ID No 1从444到462核苷酸的互补核苷酸序列
-SEQ ID No 1从442到462核苷酸的互补核苷酸序列
SEQ ID No 1从444到464核苷酸的互补核苷酸序列
-SEQ ID No 1从449到468核苷酸的互补核苷酸序列
-SEQ ID No 1从470到489核苷酸的互补核苷酸序列
-SEQ ID No 1从484到503核苷酸的互补核苷酸序列
-SEQ ID No 1从492到511核苷酸的互补核苷酸序列
-SEQ ID No 1从781到798核苷酸的互补核苷酸序列
-SEQ ID No 1从778到798核苷酸的互补核苷酸序列
-SEQ ID No 1从781到802核苷酸的互补核苷酸序列
-SEQ ID No 1从788到806核苷酸的互补核苷酸序列
-SEQ ID No 1从301到318核苷酸的互补核苷酸序列
-SEQ ID No 1从303到320核苷酸的互补核苷酸序列
-SEQ ID No 1从301到322核苷酸的互补核苷酸序列
-SEQ ID No 1从318到336核苷酸的互补核苷酸序列
-SEQ ID No 1从318到338核苷酸的互补核苷酸序列
-SEQ ID No 1从320到339核苷酸的互补核苷酸序列
-SEQ ID No 1从322到340核苷酸的互补核苷酸序列
SEQ ID No 1从322到342核苷酸的互补核苷酸序列
-SEQ ID No 1从325到343核苷酸的互补核苷酸序列
-SEQ ID No 1从329到347核苷酸的互补核苷酸序列
-SEQ ID No 1从346到365核苷酸的互补核苷酸序列
-SEQ ID No 1从348到367核苷酸的互补核苷酸序列
-SEQ ID No 1从353到371核苷酸的互补核苷酸序列
-SEQ ID No 1从378到396核苷酸的互补核苷酸序列
-SEQ ID No 1从376到396核苷酸的互补核苷酸序列
-SEQ ID No 1从382到400核苷酸的互补核苷酸序列
-SEQ ID No 1从382到401核苷酸的互补核苷酸序列
-SEQ ID No 1从384到404核苷酸的互补核苷酸序列
-SEQ ID No 1从442到459核苷酸的互补核苷酸序列
-SEQ ID No 1从440到460核苷酸的互补核苷酸序列
-SEQ ID No 1从444到461核苷酸的互补核苷酸序列
-SEQ ID No 1从442到463核苷酸的互补核苷酸序列
-SEQ ID No 1从444到465核苷酸的互补核苷酸序列
-SEQ ID No 1从469到488核苷酸的互补核苷酸序列
-SEQ ID No 1从470到488核苷酸的互补核苷酸序列
-SEQ ID No 1从484到504核苷酸的互补核苷酸序列
-SEQ ID No 1从490到509核苷酸的互补核苷酸序列
-SEQ ID No 1从491到510核苷酸的互补核苷酸序列
-SEQ ID No 1从492到512核苷酸的互补核苷酸序列
-SEQ ID No 1从561到580核苷酸的互补核苷酸序列
-SEQ ID No 1从563到582核苷酸的互补核苷酸序列
-SEQ ID No 1从565到584核苷酸的互补核苷酸序列
-SEQ ID No 1从568到587核苷酸的互补核苷酸序列
-SEQ ID No 1从572到591核苷酸的互补核苷酸序列
-SEQ ID No 1从590到609核苷酸的互补核苷酸序列
-SEQ ID No 1从640到659核苷酸的互补核苷酸序列
-SEQ ID No 1从695到713核苷酸的互补核苷酸序列
-SEQ ID No 1从782到799核苷酸的互补核苷酸序列
-SEQ ID No 1从778到799核苷酸的互补核苷酸序列
-SEQ ID No 1从788到805核苷酸的互补核苷酸序列
-SEQ ID No 1从788到808核苷酸的互补核苷酸序列
-SEQ ID No 1从318到335核苷酸的互补核苷酸序列
-SEQ ID No 1从315到336核苷酸的互补核苷酸序列
-SEQ ID No 1从320到338核苷酸的互补核苷酸序列
-SEQ ID No 1从318到339核苷酸的互补核苷酸序列
-SEQ ID No 1从322到339核苷酸的互补核苷酸序列
-SEQ ID No 1从320到340核苷酸的互补核苷酸序列
-SEQ ID No 1从325到342核苷酸的互补核苷酸序列
-SEQ ID No 1从322到343核苷酸的互补核苷酸序列
-SEQ ID No 1从329到346核苷酸的互补核苷酸序列
-SEQ ID No 1从325到346核苷酸的互补核苷酸序列
-SEQ ID No 1从329到349核苷酸的互补核苷酸序列
-SEQ ID No 1从346到364核苷酸的互补核苷酸序列
-SEQ ID No 1从348到366核苷酸的互补核苷酸序列
-SEQ ID No 1从346到366核苷酸的互补核苷酸序列
-SEQ ID No 1从348到368核苷酸的互补核苷酸序列
-SEQ ID No 1从353到370核苷酸的互补核苷酸序列
-SEQ ID No 1从378到395核苷酸的互补核苷酸序列
-SEQ ID No 1从376到397核苷酸的互补核苷酸序列
-SEQ ID No 1从376到399核苷酸的互补核苷酸序列
-SEQ ID No 1从384到401核苷酸的互补核苷酸序列
-SEQ ID No 1从384到405核苷酸的互补核苷酸序列
-SEQ ID No 1从440到461核苷酸的互补核苷酸序列
-SEQ ID No 1从466到487核苷酸的互补核苷酸序列
-SEQ ID No 1从484到505核苷酸的互补核苷酸序列
-SEQ ID No 1从490到508核苷酸的互补核苷酸序列
-SEQ ID No 1从492到509核苷酸的互补核苷酸序列
-SEQ ID No 1从491到509核苷酸的互补核苷酸序列
-SEQ ID No 1从490到510核苷酸的互补核苷酸序列
-SEQ ID No 1从491到511核苷酸的互补核苷酸序列
-SEQ ID No 1从492到513核苷酸的互补核苷酸序列
SEQ ID No 1从561到579核苷酸的互补核苷酸序列
-SEQ ID No 1从561到581核苷酸的互补核苷酸序列
-SEQ ID No 1从563到581核苷酸的互补核苷酸序列
-SEQ ID No 1从568到586核苷酸的互补核苷酸序列
-SEQ ID No 1从572到590核苷酸的互补核苷酸序列
-SEQ ID No 1从572到592核苷酸的互补核苷酸序列
-SEQ ID No 1从590到610核苷酸的互补核苷酸序列
-SEQ ID No 1从596到613核苷酸的互补核苷酸序列
-SEQ ID No 1从596到614核苷酸的互补核苷酸序列
-SEQ ID No 1从640到657核苷酸的互补核苷酸序列
-SEQ ID No 1从640到658核苷酸的互补核苷酸序列
-SEQ ID No 1从640到660核苷酸的互补核苷酸序列
-SEQ ID No 1从695到712核苷酸的互补核苷酸序列
-SEQ ID No 1从696到713核苷酸的互补核苷酸序列
-SEQ ID No 1从320到337核苷酸的互补核苷酸序列
-SEQ ID No 1从320到341核苷酸的互补核苷酸序列
-SEQ ID No 1从329到350核苷酸的互补核苷酸序列
-SEQ ID No 1从346到363核苷酸的互补核苷酸序列
-SEQ ID No 1从348到365核苷酸的互补核苷酸序列
-SEQ ID No 1从346到367核苷酸的互补核苷酸序列
-SEQ ID No 1从348到369核苷酸的互补核苷酸序列
-SEQ ID No 1从354到371核苷酸的互补核苷酸序列
-SEQ ID No 1从382到403核苷酸的互补核苷酸序列
-SEQ ID No 1从482到503核苷酸的互补核苷酸序列
-SEQ ID No 1从491到518核苷酸的互补核苷酸序列
-SEQ ID No 1从490到511核苷酸的互补核苷酸序列
-SEQ ID No 1从491到512核苷酸的互补核苷酸序列
-SEQ ID No 1从563到580核苷酸的互补核苷酸序列
-SEQ ID No 1从563到582核苷酸的互补核苷酸序列
-SEQ ID No 1从565到582核苷酸的互补核苷酸序列
-SEQ ID No 1从563到584核苷酸的互补核苷酸序列
-SEQ ID No 1从568到585核苷酸的互补核苷酸序列
-SEQ ID No 1从562到589核苷酸的互补核苷酸序列
-SEQ ID No 1从572到593核苷酸的互补核苷酸序列
-SEQ ID No 1从584到605核苷酸的互补核苷酸序列
-SEQ ID No 1从590到607核苷酸的互补核苷酸序列
-SEQ ID No 1从590到611核苷酸的互补核苷酸序列
-SEQ ID No 1从596到615核苷酸的互补核苷酸序列
-SEQ ID No 1从599到618核苷酸的互补核苷酸序列
-SEQ ID No 1从540到661核苷酸的互补核苷酸序列
-SEQ ID No 1从779到798核苷酸的互补核苷酸序列
-SEQ ID No 1从788到809核苷酸的互补核苷酸序列
-SEQ ID No 1从568到589核苷酸的互补核苷酸序列
-SEQ ID No 1从596到616核苷酸的互补核苷酸序列
-SEQ ID No 1从599到619核苷酸的互补核苷酸序列
-SEQ ID No 1从745到762核苷酸的互补核苷酸序列
-SEQ ID No 1从779到797核苷酸的互补核苷酸序列
-SEQ ID No 1从779到799核苷酸的互补核苷酸序列
-SEQ ID No 1从786到805核苷酸的互补核苷酸序列
-SEQ ID No 1从596到617核苷酸的互补核苷酸序列
-SEQ ID No 1从599到620核苷酸的互补核苷酸序列
-SEQ ID No 1从779到796核苷酸的互补核苷酸序列
-SEQ ID No 1从779到800核苷酸的互补核苷酸序列
-SEQ ID No 1从786到804核苷酸的互补核苷酸序列
-SEQ ID No 1从314到335核苷酸的互补核苷酸序列
-SEQ ID No 1从786到803核苷酸的互补核苷酸序列
-SEQ ID No 1从786到806核苷酸的互补核苷酸序列
-SEQ ID No 1从296到315核苷酸的互补核苷酸序列
c.识别外源DNA序列的引物，与识别3'侧翼序列的引物一同使用:
-SEQ ID No 2从1413到1432核苷酸的互补核苷酸序列
-SEQ ID No 2从721到740核苷酸的互补核苷酸序列
-SEQ ID No 2从767到786核苷酸的互补核苷酸序列
-SEQ ID No 2从1185到1204核苷酸的互补核苷酸序列
-SEQ ID No 2从1332到1351核苷酸的互补核苷酸序列
-SEQ ID No 2从1413到1431核苷酸的互补核苷酸序列
-SEQ ID No 2从1413到1433核苷酸的互补核苷酸序列
-SEQ ID No 2从503到522核苷酸的互补核苷酸序列
-SEQ ID No 2从507到526核苷酸的互补核苷酸序列
-SEQ ID No 2从721到739核苷酸的互补核苷酸序列
-SEQ ID No 2从722到741核苷酸的互补核苷酸序列
-SEQ ID No 2从721到741核苷酸的互补核苷酸序列
-SEQ ID No 2从770到789核苷酸的互补核苷酸序列
-SEQ ID No 2从775到794核苷酸的互补核苷酸序列
-SEQ ID No 2从1135到1154核苷酸的互补核苷酸序列
-SEQ ID No 2从1185到1202核苷酸的互补核苷酸序列
-SEQ ID No 2从1185到1205核苷酸的互补核苷酸序列
-SEQ ID No 2从1191到1210核苷酸的互补核苷酸序列
-SEQ ID No 2从1332到1350核苷酸的互补核苷酸序列
-SEQ ID No 2从1332到1352核苷酸的互补核苷酸序列
-SEQ ID No 2从1413到1430核苷酸的互补核苷酸序列
-SEQ ID No 2从506到525核苷酸的互补核苷酸序列
-SEQ ID No 2从507到525核苷酸的互补核苷酸序列
-SEQ ID No 2从507到527核苷酸的互补核苷酸序列
-SEQ ID No 2从721到738核苷酸的互补核苷酸序列
-SEQ ID No 2从722到740核苷酸的互补核苷酸序列
-SEQ ID No 2从721到742核苷酸的互补核苷酸序列
-SEQ ID No 2从775到793核苷酸的互补核苷酸序列
-SEQ ID No 2从775到795核苷酸的互补核苷酸序列
-SEQ ID No 2从1135到1153核苷酸的互补核苷酸序列
-SEQ ID No 2从1135到1155核苷酸的互补核苷酸序列
-SEQ ID No 2从1185到1206核苷酸的互补核苷酸序列
-SEQ ID No 2从1191到1209核苷酸的互补核苷酸序列
-SEQ ID No 2从1316到1335核苷酸的互补核苷酸序列
-SEQ ID No 2从1325到1344核苷酸的互补核苷酸序列
SEQ ID No 2从1332到1353核苷酸的互补核苷酸序列
-SEQ ID No 2从1413到1434核苷酸的互补核苷酸序列
-SEQ ID No 2从507到528核苷酸的互补核苷酸序列
-SEQ ID No 2从722到739核苷酸的互补核苷酸序列
-SEQ ID No 2从725到742核苷酸的互补核苷酸序列
-SEQ ID No 2从730到749核苷酸的互补核苷酸序列
-SEQ ID No 2从770到787核苷酸的互补核苷酸序列
-SEQ ID No 2从770到791核苷酸的互补核苷酸序列
-SEQ ID No 2从771到792核苷酸的互补核苷酸序列
-SEQ ID No 2从775到792核苷酸的互补核苷酸序列
-SEQ ID No 2从775到796核苷酸的互补核苷酸序列
-SEQ ID No 2从1153到1134核苷酸的互补核苷酸序列
-SEQ ID No 2从1135到1156核苷酸的互补核苷酸序列
-SEQ ID No 2从1187到1206核苷酸的互补核苷酸序列
-SEQ ID No 2从1191到1208核苷酸的互补核苷酸序列
-SEQ ID No 2从1191到1212核苷酸的互补核苷酸序列
SEQ ID No 2从1325到1343核苷酸的互补核苷酸序列
-SEQ ID No 2从1325到1345核苷酸的互补核苷酸序列
-SEQ ID No 2从1367到1384核苷酸的互补核苷酸序列
-SEQ ID No 2从1511到1528核苷酸的互补核苷酸序列
-SEQ ID No 2从506到527核苷酸的互补核苷酸序列
-SEQ ID No 2从730到750核苷酸的互补核苷酸序列
-SEQ ID No 2从1187到1204核苷酸的互补核苷酸序列
-SEQ ID No 2从1187到1205核苷酸的互补核苷酸序列
-SEQ ID No 2从1249到1266核苷酸的互补核苷酸序列
-SEQ ID No 2从1325到1342核苷酸的互补核苷酸序列
-SEQ ID No 2从1325到1346核苷酸的互补核苷酸序列
-SEQ ID No 2从730到751核苷酸的互补核苷酸序列
-SEQ ID No 2从1187到1208核苷酸的互补核苷酸序列
-SEQ ID No 2从1334到1353核苷酸的互补核苷酸序列
-SEQ ID No 2从1334到1352核苷酸的互补核苷酸序列
-SEQ ID No 2从1334到1354核苷酸的互补核苷酸序列
-SEQ ID No 2从1334到1351核苷酸的互补核苷酸序列
-SEQ ID No 2从1334到1355核苷酸的互补核苷酸序列
-SEQ ID No 2从754到771核苷酸的互补核苷酸序列
-SEQ ID No 2从842到863核苷酸的互补核苷酸序列
-SEQ ID No 2从732到751核苷酸的互补核苷酸序列
-SEQ ID No 2从732到750核苷酸的互补核苷酸序列
d.识别3'侧翼序列的引物:
-SEQ ID No 2从284到303的核苷酸序列
-SEQ ID No 2从285到305的核苷酸序列
-SEQ ID No 2从289到308的核苷酸序列
-SEQ ID No 2从160到179的核苷酸序列
-SEQ ID No 2从162到181的核苷酸序列
-SEQ ID No 2从283到303的核苷酸序列
-SEQ ID No 2从284到304的核苷酸序列
-SEQ ID No 2从286到304的核苷酸序列
-SEQ ID No 2从287到306的核苷酸序列
-SEQ ID No 2从288到308的核苷酸序列
-SEQ ID No 2从290到308的核苷酸序列
-SEQ ID No 2从394到413的核苷酸序列
-SEQ ID No 2从398到417的核苷酸序列
-SEQ ID No 2从399到418的核苷酸序列
-SEQ ID No 2从400到418的核苷酸序列
-SEQ ID No 2从119到138的核苷酸序列
-SEQ ID No 2从161到179的核苷酸序列
-SEQ ID No 2从161到181的核苷酸序列
-SEQ ID No 2从184到203的核苷酸序列
-SEQ ID No 2从192到211的核苷酸序列
-SEQ ID No 2从193到212的核苷酸序列
-SEQ ID No 2从195到214的核苷酸序列
-SEQ ID No 2从241到260的核苷酸序列
-SEQ ID No 2从283到304的核苷酸序列
-SEQ ID No 2从286到303的核苷酸序列
-SEQ ID No 2从286到306的核苷酸序列
-SEQ ID No 2从287到304的核苷酸序列
-SEQ ID No 2从287到308的核苷酸序列
-SEQ ID No 2从288到306的核苷酸序列
-SEQ ID No 2从366到385的核苷酸序列
-SEQ ID No 2从393到412的核苷酸序列
-SEQ ID No 2从395到413的核苷酸序列
-SEQ ID No 2从398到418的核苷酸序列
-SEQ ID No 2从399到417的核苷酸序列
-SEQ ID No 2从400到417的核苷酸序列
-SEQ ID No 2从401到418的核苷酸序列
-SEQ ID No 2从430到449的核苷酸序列
-SEQ ID No 2从81到100的核苷酸序列
-SEQ ID No 2从90到109的核苷酸序列
-SEQ ID No 2从159到179的核苷酸序列
-SEQ ID No 2从160到181的核苷酸序列
-SEQ ID No 2从185到203的核苷酸序列
-SEQ ID No 2从191到211的核苷酸序列
-SEQ ID No 2从192到212的核苷酸序列
-SEQ ID No 2从193到211的核苷酸序列
-SEQ ID No 2从194到212的核苷酸序列
-SEQ ID No 2从194到214的核苷酸序列
-SEQ ID No 2从196到215的核苷酸序列
-SEQ ID No 2从196到214的核苷酸序列
-SEQ ID No 2从219到238的核苷酸序列
-SEQ ID No 2从240到260的核苷酸序列
-SEQ ID No 2从242到261的核苷酸序列
-SEQ ID No 2从242到260的核苷酸序列
-SEQ ID No 2从275到294的核苷酸序列
-SEQ ID No 2从285到306的核苷酸序列
-SEQ ID No 2从289到306的核苷酸序列
-SEQ ID No 2从315到334的核苷酸序列
-SEQ ID No 2从319到338的核苷酸序列
-SEQ ID No 2从366到383的核苷酸序列
-SEQ ID No 2从372到391的核苷酸序列
-SEQ ID No 2从392到412的核苷酸序列
-SEQ ID No 2从392到413的核苷酸序列
-SEQ ID No 2从394到412的核苷酸序列
-SEQ ID No 2从397到417的核苷酸序列
-SEQ ID No 2从397到418的核苷酸序列
-SEQ ID No 2从424到443的核苷酸序列
-SEQ ID No 2从429到449的核苷酸序列
-SEQ ID No 2从431到450的核苷酸序列
-SEQ ID No 2从431到449的核苷酸序列
-SEQ ID No 2从439到458的核苷酸序列
-SEQ ID No 2从447到466的核苷酸序列
-SEQ ID No 2从481到500的核苷酸序列
-SEQ ID No 2从507到526的核苷酸序列
-SEQ ID No 2从79到96的核苷酸序列
-SEQ ID No 2从80到100的核苷酸序列
-SEQ ID No 2从82到100的核苷酸序列
-SEQ ID No 2从89到109的核苷酸序列
-SEQ ID No 2从91到109的核苷酸序列
-SEQ ID No 2从121到138的核苷酸序列
-SEQ ID No 2从184到202的核苷酸序列
-SEQ ID No 2从186到203的核苷酸序列
-SEQ ID No 2从190到211的核苷酸序列
-SEQ ID No 2从191到212的核苷酸序列
-SEQ ID No 2从193到214的核苷酸序列
-SEQ ID No 2从194到211的核苷酸序列
-SEQ ID No 2从195到215的核苷酸序列
-SEQ ID No 2从195到212的核苷酸序列
-SEQ ID No 2从218到238的核苷酸序列
-SEQ ID No 2从220到238的核苷酸序列
-SEQ ID No 2从221到238的核苷酸序列
-SEQ ID No 2从239到260的核苷酸序列
-SEQ ID No 2从241到261的核苷酸序列
-SEQ ID No 2从277到294的核苷酸序列
-SEQ ID No 2从282到303的核苷酸序列
-SEQ ID No 2从314到334的核苷酸序列
-SEQ ID No 2从316到334的核苷酸序列
-SEQ ID No 2从318到338的核苷酸序列
-SEQ ID No 2从320到338的核苷酸序列
-SEQ ID No 2从371到391的核苷酸序列
-SEQ ID No 2从391到412的核苷酸序列
-SEQ ID No 2从395到412的核苷酸序列
-SEQ ID No 2从396到417的核苷酸序列
-SEQ ID No 2从423到443的核苷酸序列
-SEQ ID No 2从430到450的核苷酸序列
-SEQ ID No 2从432到449的核苷酸序列
-SEQ ID No 2从438到458的核苷酸序列
-SEQ ID No 2从446到466的核苷酸序列
-SEQ ID No 2从448到466的核苷酸序列
-SEQ ID No 2从449到466的核苷酸序列
-SEQ ID No 2从481到498的核苷酸序列
-SEQ ID No 2从481到499的核苷酸序列
-SEQ ID No 2从482到500的核苷酸序列
-SEQ ID No 2从508到526的核苷酸序列
-SEQ ID No 2从79到100的核苷酸序列
-SEQ ID No 2从83到100的核苷酸序列
-SEQ ID No 2从88到109的核苷酸序列
-SEQ ID No 2从92到109的核苷酸序列
-SEQ ID No 2从185到202的核苷酸序列
-SEQ ID No 2从194到215的核苷酸序列
-SEQ ID No 2从217到238的核苷酸序列
-SEQ ID No 2从240到261的核苷酸序列
-SEQ ID No 2从241到262的核苷酸序列
-SEQ ID No 2从313到334的核苷酸序列
-SEQ ID No 2从317到338的核苷酸序列
-SEQ ID No 2从321到338的核苷酸序列
-SEQ ID No 2从370到391的核苷酸序列
-SEQ ID No 2从422到443的核苷酸序列
-SEQ ID No 2从428到449的核苷酸序列
-SEQ ID No 2从429到450的核苷酸序列
-SEQ ID No 2从437到458的核苷酸序列
-SEQ ID No 2从441到458的核苷酸序列
-SEQ ID No 2从445到466的核苷酸序列
-SEQ ID No 2从482到499的核苷酸序列
-SEQ ID No 2从504到523的核苷酸序列
-SEQ ID No 2从266到287的核苷酸序列
-SEQ ID No 2从446到465的核苷酸序列
-SEQ ID No 2从156到175的核苷酸序列
-SEQ ID No 2从448到465的核苷酸序列
-SEQ ID No 2从155到175的核苷酸序列
-SEQ ID No 2从157到175的核苷酸序列
-SEQ ID No 2从154到175的核苷酸序列
-SEQ ID No 2从312到329的核苷酸序列
-SEQ ID No 2从191到210的核苷酸序列
-SEQ ID No 2从190到210的核苷酸序列
-SEQ ID No 2从192到210的核苷酸序列
-SEQ ID No 2从157到176的核苷酸序列
-SEQ ID No 2从189到210的核苷酸序列
-SEQ ID No 2从193到210的核苷酸序列
-SEQ ID No 2从156到176的核苷酸序列
-SEQ ID No 2从155到176的核苷酸序列
此处所使用的“SEQ ID No.Z从位点X到位点Y的核苷酸序列”所指的核苷酸序列包括两端的核苷酸。
优选地，整合片段长度在50到500个核苷酸之间，更优选在100到350个核苷酸之间。假如错配仍然允许这些引物在最适PCR条件下特异性识别原种事件，那么特异性引物可能具有分别与原种事件的5’或3’侧翼区和原种事件的外源DNA序列同一性在80％到100％的序列。然而可允许的错配范围能够容易的通过实验确定，并且是本领域技术人员所公知的。
下面的表中列举了用所选的PCR引物对得到的预期DNA扩增子(或整合片段)的大小。
整合片段的检测可以通过多种方式，例如在凝胶分析之后估测大小。也可以直接将整合片段测序。检测扩增DNA片段的其它序列特异方法也是本领域内公知的。
由于对于原种事件，引物的序列和它们在基因组的相对位置都是唯一的，所以整合片段的扩增只在包含原种事件(的核酸)的生物样品中发生。优选地，当在未知样品中进行PCR来鉴定A5547-127的存在时，在一组引物中包含一个对照引物，用此对照引物来扩增事件植物种“看家基因”中的片段。看家基因是在多数细胞类型中表达的基因，并且其与所有细胞中共同的基础代谢活性有关。优选地，从看家基因扩增的片段比扩增的整合片段更大。依赖于被分析的样品，可以包括其它对照。
在本领域中有对标准PCR操作，例如“PCR应用指南”(Roche MolecularBiochemicals,2nd Edition,1999)的描述。在“PCR鉴定方法”中说明对于每一原种事件的PCR最适条件，包括特异性引物的序列。然而，应当理解的是，在PCR鉴定方法中许多参数都需要根据特定实验室条件来调整，并且可以被轻微的修改以获得类似的结果。例如，使用制备DNA的不同方法可能需要调整，例如所用引物的量、聚合酶和退火条件。同样地，选择其它引物可能要求PCR鉴定方法的其它最适条件。然而这些调整对于本领域技术人员是显而易见的，并且在当前PCR应用指南(例如前面所引用的)中还有详细的描述。
可选择地，可以使用特异引物扩增整合片段，该整合片段可用作在生物样品中鉴定A5547-127的“特异性探针”。在允许探针和核酸中其相应片段杂交的条件下，用探针与生物样品的核酸接触，导致核酸/探针杂交体的形成。此杂交体的形成可以被检测到(例如标记核酸或探针)，由此此种杂交体的形成表明A5547-127的存在。在本领域内已有描述关于此种基于与特异探针(或者在固相载体上，或者在溶液中)杂交的鉴定方法。特异探针优选在最适条件下，能特异性与原种事件5’或3’侧翼区且优选也包含与其连接的外源DNA的区域(在下文中称为“特异区域”)的部分杂交的序列。优选地，特异探针包含长度在50到500bp之间的序列，优选100到350bp，其与特异区域的核苷酸序列至少有80％同一性(或互补)，优选在80％到85％之间，更优选在85％到90％之间，尤其优选在90％到95％之间，最优选在95％到100％之间。优选地，特异探针包含与原种事件的特异区域一致(或互补)的约15到约100个连续的核苷酸。
此处使用的“试剂盒”指以实施本发明的方法为目的的一组试剂，更具体地，用于在生物样品中鉴定原种事件A5547-127。更具体地，如前所述，本发明的试剂盒优选的实施方案包含至少一个或两个特异性引物。任选地，试剂盒还包含此处所描述的在PCR鉴定方法中的任何其它试剂。可选择地，根据本发明的其它实施方案，试剂盒包含如前所述的特异性探针，其与生物样品的核酸特异杂交，以鉴定其中A5547-127的存在。任选地，试剂盒还包含使用特异性探针在生物样品中鉴定A5547-127的任何其它试剂(例如但不限于杂交缓冲液、标记物)。
可以使用本发明的试剂盒，且可以特异性调整其组分，用于质量控制(例如多批种子)、测定在植物材料、或包含植物材料或由植物材料衍生的材料(例如但是不限于食物或饲料产品)中的原种事件。
此处所使用的关于核酸序列(DNA或RNA)的“序列同一性”指一致的核苷酸位置数除以两个序列中较短序列的核苷酸数。两个核苷酸序列的比对通过Wilbur和Lipmann算法进行(Wilbur和Lipmann,1983,Proc.Nat.Acad.Sci.USA80:726)，所使用的窗口大小为20个核苷酸，字长为4个核苷酸，并且空位罚分为4。计算机辅助分析和序列数据译码，包括如前所述的序列比对，能够例如使用IntelligeneticsTM Suite(Intelligenetics Inc.,CA)程序或者遗传计算机组(GCG,University of Wisconsin Biotechnology center)的序列分析软件包方便地进行。具有至少约75％，特别至少约80％，更特别至少85％，很特别约90％，尤其约95％，更尤其约100％序列同一性的序列被表示为“基本相似”。很明显当RNA序列被称为与DNA序列基本相似或有一定程度序列同一性时，DNA序列中的脱氧胸腺嘧啶(T)与RNA序列中的尿嘧啶(U)被认为是等同的。
此处所使用的术语“引物”包含在依赖模板的方法(例如PCR)中，任何能够启动新生核酸合成的核酸。通常，引物是从10到30个核苷酸的寡核苷酸，但是也可以使用更长的序列。尽管优选单链形式的引物，但是引物可以以双链形式提供。探针可以用作引物，但是其应依照结合靶DNA或RNA而设计，并且不需用于扩增方法中。
此处所用术语“识别”，当指特异引物时，指的是在方法中的起始条件下(例如PCR鉴定方法的条件)，特异引物与原种事件中的核酸序列特异性杂交，其中通过阳性和阴性对照的存在来确定特异性。
此处所用术语“杂交”，当指特异性探针时，所指的是在标准严格条件下，探针与原种事件的核酸序列中特异区域结合。此处所用的标准严格条件指此处描述的杂交条件或Sambrook等人1989(Molecular Cloning:A Laboratory Manual,Second Edition,ColdSpring Harbour Laboratory Press,NY)所描述的常规杂交条件，其例如可以包含如下步骤：1)将植物基因组DNA片段固定在滤膜上，2)滤膜于42℃在50％甲酰胺、5×SSPE、2×Denhardt试剂和0.1％SDS中预杂交1到2小时，或于68℃在6×SSC、2×Denhardt试剂和0.1％SDS中预杂交1到2小时，3)加入已标记的杂交探针，4)培育16到24小时，5)在1×SSC、0.1％SDS中室温清洗滤膜20分钟，6)清洗滤膜三次，每次在0.2×SSC、0.1％SDS中68℃清洗20分钟，和7)滤膜在-70℃用增光屏向X光片曝光24到48小时。
此处所使用的生物样品是植物、植物材料或包含植物材料的产品的样品。术语“植物”预期包括在任何成熟期的大豆(Glycine max)植物组织、以及包括从任何此类植物得来的或衍生的任何细胞、组织或器官，包括但不限于任何种子、叶、茎、花、根、单细胞、配子、细胞培养物、组织培养物或原生质体。此处所使用的“植物材料”指从植物获得或衍生的材料。包含植物材料的产品指食物、饲料、或其它用植物材料生产的产品或可能被植物材料污染的产品。在本发明中，应该理解的是测试此类生物样品中A5547-127的特异性核酸的存在，暗示样品中核酸的存在。因此此处的方法指在生物样品中鉴定原种事件A5547-127，涉及在生物样品中包含原种事件的核酸的鉴定。
此处所使用的“包含”解释为在提到的说明特征、整体、步骤、试剂或组分中特异性存在，但是不排除一个或多个特征、整体、步骤或组分、或其组的存在或添加。因此，例如包含核苷酸或氨基酸序列的核酸或蛋白质，可能比目前所引用的包含更多的核苷酸或氨基酸，即包含于更大的核酸或蛋白质中。包含功能上或结构上确定的DNA序列的嵌合基因可能包含附加的DNA序列等。
本发明也涉及大豆中原种事件A5547-127发育为包含此事件的植物、从这些植物获得的子代和植物细胞、或由此事件衍生的植物材料。包含原种事件A5547-127的植物通过实施例1中的描述来获得。
包含A5547-127的大豆植物或植物材料可以根据实施例2所描述的A5547-127的PCR鉴定方法来鉴定。简而言之，使用特异性识别A5547-127的5’或3’侧翼序列的引物(例如具有SEQ ID NO:15序列的引物)，以及使用识别外源DNA中序列的引物(例如具有SEQ IDNO:13序列的引物)通过PCR来扩增生物样品中存在的大豆基因组DNA。使用扩增部分内源大豆序列的DNA引物作为PCR扩增的阳性对照。如果应用PCR扩增，材料产生预期大小的片段，那么材料则包含来自含有原种事件A5547-127的大豆植物的植物材料。
含有A5547-127的植物其特征在于它们的除草剂(glufosinate)耐受性，在本发明中，其包括耐受除草剂LibertyTM的植物。LibertyTM耐受性可以通过不同方法测试。当需要区别抗性和敏感性植物时，此处所描述的叶涂布方法是最有用的，其不会杀死敏感性植物。可选地，可以通过LibertyTM喷雾应用来测试耐受性。为了得到最好的结果，喷雾处理应该在V3和V4叶期之间使用。耐受性植物的特征在于用至少200克活性成分/公顷(g.a.i./ha)，优选400g.a.i./ha，和可能直到1600g.a.i./ha(4×正常田地比例)对植物进行喷雾，而不会杀死植物。散播应用应当使用比例为28-34盎司LibertyTM。最好以每英亩20加仑体积水应用，使用扁的扇形喷嘴，小心不要直接喷雾应用于整个植物以避免灼伤叶表面。除草剂的作用应该在48小时内出现，并且在5-7天内清楚可见。
含有A5547-127的植物的特征还在于通过PAT测定(De Block等人,1987)确定在其细胞中存在膦丝菌素乙酰基转移酶。
含有A5547-127的植物的特征也在于其具有的农艺学特性，在缺乏杂草压力和使用LibertyTM作为杂草控制的条件下，与美国可商购的多种大豆具有可比性。参照包含此事件的植物特定的令人感兴趣的表型和分子特性，在此处描述的大豆植物基因组的插入区域中已经观察到外源DNA的存在。更具体地，在这些植物基因组的此特殊区域中存在外源DNA，导致其在植物中展现目的基因稳定表型表达而所希望的该植物的农艺学性没有任何方面的显著破坏。
以下实例描述在生物样品中鉴定原种事件A5547-127的发展手段的鉴定。
除非另外说明，所有重组DNA技术都根据Sambrook等人(1989)分子克隆:实验室操作手册,第二版,Cold Spring Harbour Laboratory Press,NY以及在Ausubel等人(1994)Current Protocols in Molecular Biology,Current Protocols,USA的第1卷和第2卷中所描述的标准方法进行。植物分子操作的标准材料和方法在由BIOS ScientificPublications Ltd(UK)和Blackwell Scientific Publications,UK出版的R.D.D.Croy的Plant Molecular Biology Labfax(1993)中被描述。
在说明书和实施例中，如下序列用作参考：
SEQ ID No.1:包含A5547-127的5'侧翼区的核苷酸序列
SEQ ID No.2:包含A5547-127的3'侧翼区的核苷酸序列
SEQ ID No.3:引物HCA150
SEQ ID No.4:引物DPA013
SEQ ID No.5:引物DPA228
SEQ ID No.6:引物KVM173
SEQ ID No.7:引物YTP228
SEQ ID No.8:引物YTP220
SEQ ID No.9:引物DPA024
SEQ ID No.10:引物YTP245
SEQ ID No.11:引物YTP170
SEQ ID No.12:引物YTP227
SEQ ID No.13:引物MDB688
SEQ ID No.14:引物KVM175
SEQ ID No.15:引物MDB687
SEQ ID No.16:引物SMO022
SEQ ID No.17:引物SMO024
SEQ ID No.18:用于扩增对照片段的引物1
SEQ ID No.19:用于扩增对照片段的引物2
附图简述
以下实施例并不预期将本发明限制于所述特定实施方案中，其应与附图结合在一起去理解，并在此处引用作为参考，其中：
图1:引用的核苷酸序列与引物之间关系的图示，黑色条：外源DNA；亮条：植物起源的DNA；条下面的数字代表核苷酸位置；(c)指所示核苷酸序列的互补序列。
图2:为A5547-127发展的PCR鉴定方法。凝胶上样顺序：第1泳道：来自包含转基因事件A5547-127的大豆植物的DNA样品；第2泳道：来自不包含原种事件A5547-127的转基因大豆植物的DNA样品；第3泳道:来自野生型大豆植物的对照DNA样品；第4泳道：无模板对照；第5泳道：分子量标记。
实施例
1.原种事件A5547-127侧翼区的鉴定
通过用包含pat基因(编码膦丝菌素乙酰基转移酶)编码序列的载体转化大豆，在花椰菜花叶病毒组成型35S启动子的控制下，产生除草剂抗性的大豆。
基于广泛筛选操作选出原种事件A5547-127，此操作基于除草剂抗性基因的良好表达和稳定性，及其与最适农艺学特性的相容性。
在A5547-127事件中外源DNA的侧翼区序列通过Liu等人(1995,Plant J.8(3):457-463)所描述的热不均匀交织(TAIL-)PCR方法来测定。此方法利用连续反应中三个嵌套引物，与较短的任意简并引物一起使用，以便能够热控制特异和非特异产物的相对扩增效率。选择特异引物与外源DNA的两侧退火，并且基于它们的退火条件来选择。少量(5μl)未纯化的二级和三级PCR产物在1％琼脂糖凝胶上分析。三级PCR产物用于预备性扩增、纯化、并使用DyeDeoxy终止循环试剂盒在自动测序仪上测序。
1.1.右(5')侧翼区
通过TAIL-PCR方法获得的经鉴定包含5’侧翼区的片段被全部测序(SEQ IDNo.1)。在1到311核苷酸之间的序列相应于植物DNA，而312到810核苷酸之间的序列相应于外源DNA。
1.2.左(3')侧翼区
通过TAIL-PCR方法获得的经鉴定包含3’侧翼区的片段被全部测序(SEQ IDNo.2)。1到509核苷酸之间的序列相应于外源DNA，而510到1880核苷酸之间的序列相应于植物DNA。
2.多聚酶链式反应鉴定方法的发展
2.1.引物
发展了识别原种事件内序列的特异性引物。更特别地，发展了识别A5547-127的3’侧翼区内序列的引物。随后在外源DNA的序列内选择第二引物，以便引物跨越约150核苷酸的序列。找到的以下引物在A5547-127DNA的PCR反应中给出特定明确和可重复的结果:
MDB687:5'-TgT.ggT.TAT.ggC.ggT.gCC.ATC-3'(SEQ ID No.:15)(靶序列:植物DNA)
MDB688:5'-TgC.TAC.Agg.CAT.CgT.ggT.gTC-3'(SEQ ID No.:13)(靶序列:插入DNA)
靶向内源序列的引物优选包含于PCR混合物中。这些引物在未知样品和DNA阳性对照中用作内对照。使用内源引物对得到的阳性结果证明在为产生PCR产物而制备的基因组DNA中含有足够的丰富的DNA。选择识别大豆中的看家基因的内源引物：
SOY01:5'-gTC.AgC.CAC.ACA.gTg.CCT.AT-3'(SEQ ID No.:20)(位于大豆肌动蛋白1基因(登录号JO 1298))
SOY02:5'-gTT.ACC.gTA.CAg.gTC.TTT.CC-3'(SEQ ID No.:21)(位于大豆肌动蛋白1基因(登录号JO 1298))
2.2.扩增片段
在PCR反应中预期的扩增片段：
对于引物对SOY01-SOY02:413bp(内源对照)
对于引物对MDB688-MDB687:151bp(A5547-127原种事件)
2.3.模板DNA
根据Edwards等人(Nucleic Acid Research,19,pi 349,1991)的描述通过叶打孔制备模板DNA。当用其它方法制备DNA时，应该利用不同量的模板进行一轮检测。通常50ng的基因组模板DNA产生最好结果。
2.4.指定的阳性和阴性对照
为了避免假阳性或假阴性，确定在一轮PCR中应该包括如下阳性和阴性对照:
-主要混合物对照(DNA阴性对照)。此PCR反应中不加入DNA。当检测到预期结果，即没有PCR产物时，表明PCR混合物没有被靶DNA污染。
-DNA阳性对照(已知包含转基因序列的基因组DNA样品)。阳性对照的成功扩增说明PCR在允许靶序列扩增的条件下进行。
-野生型DNA对照。此PCR中提供的模板DNA是从非转基因植物中制备的基因组DNA。当检测到预期结果，即没有检测到转基因PCR产物扩增，但是检测到内源PCR产物的扩增时，表明在基因组DNA样品中没有可检测到的转基因背景扩增。
2.5.PCR条件
在如下条件下获得最优结果：
-25μl反应的PCR混合物含有:
2.5μl模板DNA
2.5μl 10×扩增缓冲液(与Taq聚合酶一起提供)
0.5μl 10mM dNTP
0.5μl MDB688(10pm/μl)
0.5μl MDB687(10pm/μl)
0.25μl SOY01(10pm/μl)
0.25μl SOY02(10pm/μl)
0.1μl Taq DNA聚合酶(5单位/μl)
加水至25μl
-为得到最优结果，随后的热循环步骤如下:
95℃，4分钟
随后:95℃，1分钟
57℃，1分钟
72℃，2分钟
5个循环
随后:92℃，30秒
57℃，30秒
72℃，1分钟
25个循环
随后:72℃，5分钟
2.6.琼脂糖凝胶分析
为了达到PCR的最佳可视结果，确定应当在1.5％琼脂糖凝胶(Tris-硼酸盐缓冲液)上应用10到20μl之间的PCR样品，以及适当的分子量标记(例如100bp ladderPHARMACIA)。
2.7.结果验证
已确定来自单轮PCR和单个PCR混合物中转基因植物DNA样品的数据是不可接受的，除非1)DNA阳性对照显示预期的PCR产物(转基因和内源片段)，2)DNA阴性对照的PCR扩增是阴性的(没有片段)和3)野生型DNA对照显示预期结果(内源片段扩增)。
当随后进行上述A5547-127的PCR鉴定方法时，条带显示可见量的预期大小的转基因和内源PCR产物，这表明相应的制备基因组模板DNA的植物具有遗传的A5547-127原种事件。条带没有显示可见量的转基因PCR产物，而是显示可见量的内源PCR产物，表明相应的制备基因组模板DNA的植物不包含原种事件。条带没有显示可见量的内源和转基因PCR产物，表明基因组DNA的质量和/或数量不能够产生PCR产物。这些植物不能被记入。应当重新制备基因组DNA，并且在合适的条件下进行新一轮PCR。
2.8.使用差别PCR(discriminating PCR)方法鉴定A5547-127
在尝试未知筛选之前，用所有合适的对照进行一轮测试。发展的方法可能需要将各实验室之间不同的组分进行最优化(模板DNA制备、Taq DNA聚合酶、引物质量、dNTP's、热循环仪等)。
内源序列的扩增在方法中起关键作用。从已知的转基因基因组DNA模板中可以获得扩增等摩尔量的内源和转基因序列的PCR和热循环条件。每当靶内源片段不被扩增或者没有以同样的溴化乙啶染色强度被扩增时(如由琼脂糖凝胶电泳判断)，可能需要优化PCR条件。
根据上述方法测试来自多个植物的大豆叶材料，其中的一些包含A5547-127。来自原种事件A5547-127和来自大豆野生型的样品分别作为阳性和阴性对照。
图2说明在多个大豆植物样品中对A5547-127的原种事件PCR鉴定方法所获得的结果(1-14泳道)。第1泳道中的样品检测到151bp条带说明其包含原种事件，而第2、3和4泳道中的样品不包含A5547-127。第2泳道包含另一大豆原种事件，第3泳道代表非转基因大豆对照；第4泳道代表阴性对照(水)样品，且第5泳道代表分子量标记(100bp)。
3.使用特异整合片段作为探针检测包含A5547-127的材料
A5547-127的特异整合片段通过使用特异引物MDB687(SEQ ID No.15)和MDB688(SEQ ID No.13)进行PCR扩增而获得，或者通过化学合成获得，并且被标记。此整合片段用作在生物样品中检测A5547-127的特异性探针。根据标准操作从样品中提取核酸。此核酸随后在杂交条件下与特异性探针接触，优化该条件使允许形成杂交体。随后检测到杂交体的形成表明在样品中存在A5547-127核酸。任选地，样品中的核酸在用特异性探针接触之前，先用特异引物扩增。可选择地，用特异性探针而不是整合片段接触核酸之前，先标记核酸。任选地，在接触样品之前，特异性探针被附加到固体载体上(例如，但是不限于，滤膜、条带或珠子)。
本申请涉及如下实施方案：
1.在生物样品中鉴定原种事件A5547-127的方法，该方法包括用特异性识别A5547-127的5’或3’侧翼区的特异性引物或探针检测A5547-127特定区域。
2.实施方案1的方法，所述方法包括使用聚合酶链式反应，用至少两种引物扩增存在于所述生物样品的核酸从100到500碱基对之间的DNA片段，所述引物之一识别A5547-127的5’侧翼区，所述5’侧翼区具有SEQ ID No 1从1到311核苷酸的核苷酸序列，或者识别A5547-127的3’侧翼区，所述3’侧翼区具有SEQ ID No 2从510到1880核苷酸的互补核苷酸序列，所述引物的另一个识别外源DNA中的序列，该外源DNA具有SEQ ID No 1从312到810核苷酸的互补核苷酸序列，或者SEQ ID No 2从510到1880核苷酸的核苷酸序列。
3.实施方案2的方法，其中识别5’侧翼区的所述引物由17到200个连续核苷酸的核苷酸序列组成，其选自SEQ ID No 1从1到311核苷酸的核苷酸序列；或者识别A5547-127的3’侧翼区的所述引物由17到200个连续核苷酸的核苷酸序列组成，其选自SEQ ID No 2从510到1880核苷酸的互补核苷酸序列；并且识别外源DNA中的序列的所述引物由17到200个连续核苷酸的核苷酸序列组成，其选自SEQ ID No 1从312到810核苷酸的互补核苷酸序列，或者选自SEQ ID No 2从510到1880核苷酸的核苷酸序列。
4.实施方案2的方法，其中识别5’侧翼区的所述引物在其3’最末端包含至少17个连续核苷酸的核苷酸序列，其选自SEQ ID No 1从1到311核苷酸的核苷酸序列；或者识别A5547-127的3’侧翼区的所述引物在其3’最末端包含至少17个连续核苷酸的核苷酸序列，其选自SEQ ID No 2从510到1880核苷酸的互补核苷酸序列；并且识别外源DNA内序列的所述引物在其3’端包含至少17个连续核苷酸的核苷酸序列，其选自SEQ ID No 1从312到810核苷酸的互补核苷酸序列，或者选自SEQ ID No 2从510到1880核苷酸的核苷酸序列。
5.实施方案4的方法，其中所述引物分别包含SEQ ID No.13和SEQ ID No.15的序列。
6.实施方案5的方法，该方法包括用A5547-127鉴定方案扩增约151碱基对的片段。
7.用于在生物样品中鉴定原种事件A5547-127的试剂盒，所述试剂盒包含一个识别A5547-127的5’侧翼区的引物，所述5’侧翼区具有SEQ ID No 1从1到311核苷酸的核苷酸序列；或者一个识别A5547-127的3’侧翼区的引物，所述3’侧翼区具有SEQ ID No 2从510到1880核苷酸的互补核苷酸序列；以及一个识别外源DNA中的序列的引物，所述外源DNA具有SEQ ID No 1从312到810核苷酸的互补核苷酸序列，或SEQ ID No 2从510到1880核苷酸的核苷酸序列。
8.实施方案7的试剂盒，其中识别5’侧翼区的所述引物由17到200个连续核苷酸的核苷酸序列组成，其选自SEQ ID No 1从1到311核苷酸的核苷酸序列；或者识别A5547-127的3’侧翼区的所述引物由17到200个连续核苷酸的核苷酸序列组成，其选自SEQ ID No 2从510到1880核苷酸的互补核苷酸序列；并且识别外源DNA内序列的所述引物由17到200个连续核苷酸组成，其选自SEQ ID No 1从312到810核苷酸的互补核苷酸序列，或者SEQ ID No2从510到1880核苷酸的核苷酸序列。
9.实施方案7的试剂盒，其中识别5’侧翼区的所述引物在其3’最末端包含至少17个连续核苷酸的核苷酸序列，其选自SEQ ID No 1从1到311核苷酸的核苷酸序列；或者识别A5547-127的3’侧翼区的所述引物在其3’最末端包含至少17个连续核苷酸的核苷酸序列，其选自SEQ ID No 2从510到1880核苷酸的互补核苷酸序列；并且识别外源DNA内序列的所述引物在其3’端包含至少17个连续的核苷酸，其选自SEQ ID No 1从312到810核苷酸的互补核苷酸序列，或者选自SEQ ID No 2从510到1880核苷酸的核苷酸序列。
10.实施方案7的试剂盒，其包含由SEQ ID No.13的序列组成的引物和由SEQ IDNo.15的序列组成的引物。
11.在A5547-127PCR鉴定方案中使用的引物，其具有的序列在最优化的PCR条件下特异性识别A5547-127的5’或3’侧翼区内的序列，所述5’侧翼区具有SEQ ID No 1从1到311核苷酸的核苷酸序列，并且所述3’侧翼区具有SEQ ID No 2从510到1880核苷酸的互补核苷酸序列。
12.实施方案11的引物，其中所述引物由17到200个连续核苷酸的核苷酸序列组成，其选自SEQ ID No 1从1到311核苷酸的核苷酸序列，或者选自SEQ ID No 2从510到1880核苷酸的互补核苷酸序列。
13.实施方案11的引物，其中所述引物在其3’最末端包含至少17个连续核苷酸的核苷酸序列，其选自SEQ ID No 1从1到311核苷酸的核苷酸序列，或者选自SEQ ID No 2从510到1880核苷酸的互补核苷酸序列。
14.在其3’最末端包含SEQ ID No.13序列的引物。
15.在其3’最末端包含SEQ ID No.15序列的引物。
16.实施方案1的方法，该方法包括用A5547-127的特异性探针与生物样品的核酸杂交。
17.实施方案16的方法，其中所述特异性探针的序列与包含A5547-127的5’侧翼序列或3’侧翼序列及其邻接的外源DNA序列的部分序列具有至少80％的序列同一性。
18.实施方案17的方法，其中所述特异性探针的序列与SEQ ID No.1从260到360核苷酸或SEQ ID No.2从460到560核苷酸、或所述序列的互补序列具有至少80％的序列同一性。
19.用于在生物样品中鉴定原种事件A5547-127的试剂盒，所述试剂盒包含特异性探针，其能够与A5547-127的特定区域特异性杂交。
20.实施方案19的试剂盒，其中所述特异性探针的序列与包含A5547-127的5’侧翼序列或3’侧翼序列及其邻接的外源DNA序列的部分序列具有至少80％的序列同一性。
21.实施方案20的试剂盒，其中所述特异性探针的序列与SEQ ID No.1从260到360的核苷酸或SEQ ID No.2从460到560的核苷酸、或所述序列的互补序列具有至少80％的序列同一性。
22.用于在生物样品中鉴定原种事件A5547-127的特异性探针。
23.实施方案22的探针，其与包含A5547-127的5’侧翼序列或3’侧翼序列及其邻接的外源DNA序列、或其互补序列的部分序列具有至少80％的序列同一性。
24.实施方案23的探针，其与SEQ ID No.1从260到360的核苷酸或SEQ ID No.2从460到560的核苷酸、或所述序列的互补序列具有至少80％的序列同一性。
25.用于鉴定生物样品中原种事件A5547-127的特异性探针，其序列与SEQ IDNo.1从260到360的核苷酸或SEQ ID No.2从460到560的核苷酸、或所述序列的互补序列基本相似。
26.用于确定种子纯度的方法，该方法包括在种子样品中用特异性引物或探针检测A5547-127的特定区域，所述引物或探针特异性识别A5547-127的5’或3’侧翼区。
27.用于筛选存在A5547-127的种子的方法，该方法包括在大批种子样品中用特异性引物或探针检测A5547-127的特定区域，所述引物或探针特异性识别A5547-127的5’或3’侧翼区。
28.大豆植物、或其细胞、部分、种子或子代，在其基因组中包含原种事件A5547-127。

Claims (3)

1.一种DNA，其是序列SEQ ID NO:2的第377位核苷酸至第527位核苷酸。
2.一种DNA，其是SEQ ID NO:2的序列。
3.一种DNA，其是SEQ ID NO:1的序列。
CN201510514913.XA 2005-04-11 2006-04-04 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒 Active CN105112520B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP05075846 2005-04-11
EP05075846.5 2005-04-11
US67041405P 2005-04-12 2005-04-12
US60/670,414 2005-04-12
CN200680011600.1A CN101155933B (zh) 2005-04-11 2006-04-04 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN200680011600.1A Division CN101155933B (zh) 2005-04-11 2006-04-04 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒

Publications (2)

Publication Number Publication Date
CN105112520A CN105112520A (zh) 2015-12-02
CN105112520B true CN105112520B (zh) 2019-05-28

Family

ID=39256903

Family Applications (2)

Application Number Title Priority Date Filing Date
CN200680011600.1A Active CN101155933B (zh) 2005-04-11 2006-04-04 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒
CN201510514913.XA Active CN105112520B (zh) 2005-04-11 2006-04-04 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN200680011600.1A Active CN101155933B (zh) 2005-04-11 2006-04-04 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒

Country Status (14)

Country Link
US (2) US8017756B2 (zh)
EP (1) EP1871901B1 (zh)
JP (1) JP5281392B2 (zh)
CN (2) CN101155933B (zh)
AT (1) ATE514792T1 (zh)
BR (1) BRPI0610462B1 (zh)
CA (1) CA2603949C (zh)
DK (1) DK1871901T3 (zh)
ES (2) ES2369032T3 (zh)
MX (1) MX2007012561A (zh)
PL (1) PL1871901T3 (zh)
PT (1) PT1871901E (zh)
SI (1) SI1871901T1 (zh)
WO (1) WO2006108675A2 (zh)

Families Citing this family (704)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9078406B2 (en) 2008-08-29 2015-07-14 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event MON87754 and methods for detection thereof
AR080946A1 (es) * 2009-11-23 2012-05-23 Bayer Bioscience Nv Evento elite ee-gm3 de soja y metodos y conjuntos de elementos para identificar dicho evento en muestras biologicas
CN103003443B (zh) * 2009-11-23 2016-09-21 拜尔作物科学股份有限公司 耐除草剂大豆植物及鉴定其的方法
EP2338520A1 (de) 2009-12-21 2011-06-29 Ludwig Maximilians Universität Konjugat mit Zielfindungsligand und dessen Verwendung
EP2525658B1 (de) 2010-01-22 2017-03-01 Bayer Intellectual Property GmbH Akarizide und/oder insektizide wirkstoffkombinationen
CN103313971B (zh) 2010-11-15 2015-12-02 拜耳知识产权有限责任公司 N-芳基吡唑（硫代）甲酰胺
EP2646413A1 (de) 2010-11-29 2013-10-09 Bayer Intellectual Property GmbH Alpha-beta-ungesättigte imine
EP2460407A1 (de) 2010-12-01 2012-06-06 Bayer CropScience AG Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe
AP3519A (en) 2010-12-01 2016-01-11 Bayer Ip Gmbh Use of fluopyram for controlling nematodes in crops and for increasing yield
CA2819684C (en) 2010-12-03 2024-05-07 Dow Agrosciences Llc Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
WO2012120105A1 (en) 2011-03-10 2012-09-13 Bayer Cropscience Ag Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds
EP3292760A1 (en) 2011-03-23 2018-03-14 Bayer Intellectual Property GmbH Active compound combinations
US20140051575A1 (en) 2011-04-08 2014-02-20 Juergen Benting Fungicide hydroximoyl-tetrazole derivatives
TR201901837T4 (tr) 2011-04-22 2019-03-21 Bayer Cropscience Ag Bir (tiyo)karboksamit türevi ve bir mantar öldürücü bileşik içeren etkin bileşik terkipleri.
WO2012171914A1 (en) 2011-06-14 2012-12-20 Bayer Intellectual Property Gmbh Use of an enaminocarbonyl compound in combination with a biological control agent
TW201317353A (zh) 2011-07-13 2013-05-01 Dow Agrosciences Llc 具疊加除草劑耐受性之品件８２６４．４２．３２．１，相關基因轉殖大豆品系以及測定該品件的方法
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
CN103981149A (zh) 2011-08-22 2014-08-13 拜尔作物科学公司 修饰植物基因组的方法和手段
EP2561759A1 (en) 2011-08-26 2013-02-27 Bayer Cropscience AG Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth
JP6002225B2 (ja) 2011-09-12 2016-10-05 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ 殺菌性４−置換−３−｛フェニル［（ヘテロシクリルメトキシ）イミノ］メチル｝−１，２，４−オキサジアゾール−５（４ｈ）−オン誘導体
JP6138797B2 (ja) 2011-09-16 2017-05-31 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ 植物収量を向上させるためのアシルスルホンアミド類の使用
BR112014006208B1 (pt) 2011-09-16 2018-10-23 Bayer Intellectual Property Gmbh método de indução de respostas reguladoras do crescimento nas plantas aumentando o rendimento de plantas úteis ou plantas de cultura e composição de aumento do rendimento da planta compreendendo isoxadifen-etilo ou isoxadifeno e combinação de fungicidas
IN2014CN01860A (zh) 2011-09-16 2015-05-29 Bayer Ip Gmbh
PL2764101T3 (pl) 2011-10-04 2017-09-29 Bayer Intellectual Property Gmbh RNAi do kontroli grzybów i lęgniowców poprzez hamowanie genu dehydrogenazy sacharopinowej
EP2782920B1 (en) 2011-11-21 2016-12-21 Bayer Intellectual Property GmbH Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives
EP2785698B1 (en) 2011-11-30 2018-10-10 Bayer Intellectual Property GmbH Fungicidal n-bicycloalkyl and n-tricycloalkyl (thio)carboxamide derivatives
US9414595B2 (en) 2011-12-19 2016-08-16 Bayer Cropscience Ag Use of anthranilic acid diamide derivatives for pest control in transgenic crops
BR112014015993A8 (pt) 2011-12-29 2017-07-04 Bayer Ip Gmbh composto, composição, método para o controle dos fungos, utilização dos compostos e processo para a produção das composições
MX343871B (es) 2011-12-29 2016-11-25 Bayer Ip Gmbh Derivados de 3-[(piridin-2-ilmetoxiimino)(fenil)metil]-2-sustituid o-1,2,4-oxadiazol-5(2h)-ona fungicidas.
CN104507314B (zh) 2012-01-25 2018-01-09 拜耳知识产权有限责任公司 含有氟吡菌酰胺芽孢杆菌和生物防治剂的活性化合物结合物
US20150011389A1 (en) 2012-01-25 2015-01-08 Bayer Intellectual Property Gmbh Active Compound Combinations Containing Fluopyram and Biological Control Agent
PL2819518T3 (pl) 2012-02-27 2018-02-28 Bayer Intellectual Property Gmbh Kombinacje związku aktywnego zawierające tiazoiloizoksazolin i fungicyd
WO2013139949A1 (en) 2012-03-23 2013-09-26 Bayer Intellectual Property Gmbh Compositions comprising a strigolactame compound for enhanced plant growth and yield
US9357778B2 (en) 2012-04-12 2016-06-07 Bayer Cropscience Ag N-acyl-2-(cyclo)alkypyrrolidines and piperidines useful as fungicides
MX2014012449A (es) 2012-04-20 2015-01-19 Bayer Cropscience Ag Derivados de n-cicloalquil-n-[(heterociclilfenil)metilen]-(tio) carboxamida.
JP2015516396A (ja) 2012-04-20 2015-06-11 バイエル・クロップサイエンス・アーゲーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ａｇ Ｎ−シクロアルキル−ｎ−［（三置換シリルフェニル）メチレン］−（チオ）カルボキサミド誘導体
MX2014013489A (es) 2012-05-09 2015-02-12 Bayer Cropscience Ag 5-halogenopirazolindanil carboxamidas.
EP2662364A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole tetrahydronaphthyl carboxamides
EP2662361A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol indanyl carboxamides
EP2662370A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole benzofuranyl carboxamides
EP2662362A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole indanyl carboxamides
EP2662363A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole biphenylcarboxamides
EP2662360A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole indanyl carboxamides
EP2847170B1 (en) 2012-05-09 2017-11-08 Bayer CropScience AG Pyrazole indanyl carboxamides
AR091104A1 (es) 2012-05-22 2015-01-14 Bayer Cropscience Ag Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida
AU2013269661B2 (en) 2012-05-30 2016-10-27 Bayer Cropscience Ag Compositions comprising a biological control agent and an insecticide
CN104507318B (zh) 2012-05-30 2018-12-25 拜尔农作物科学股份公司 包括生物防治剂和来自呼吸链复合体iii抑制剂的杀真菌剂的组合物
KR102095979B1 (ko) 2012-05-30 2020-04-02 바이엘 크롭사이언스 악티엔게젤샤프트 생물학적 방제제 및 살곤충제를 포함하는 조성물
EP3488700B1 (en) 2012-05-30 2020-12-16 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
WO2013178649A1 (en) 2012-05-30 2013-12-05 Bayer Cropscience Ag Compositions comprising a biological control agent and a fungicide from the group consisting of inhibitors of the respiratory chain at complex i or ii.
WO2013178653A1 (en) 2012-05-30 2013-12-05 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of atp production and inhibitors of the cell wall synthesis
PL2854549T3 (pl) 2012-05-30 2019-02-28 Bayer Cropscience Ag Kompozycja zawierająca środek kontroli biologicznej i fluopikolid
TR201816247T4 (tr) 2012-05-30 2018-11-21 Bayer Cropscience Ag Metalaksil ve metalaksil-m'den seçilen bir fungisit ve bir biyolojik kontrol ajanı içeren bileşim.
ES2702303T3 (es) 2012-07-31 2019-02-28 Bayer Cropscience Ag Composiciones plaguicidas que comprenden una mezcla de terpeno y flupiradifurona
UY35035A (es) 2012-09-14 2014-04-30 Bayer Cropscience Ag ?molécula de ácido nucleico recombinante que codifica una enzima hppd, vector, célula huésped, semilla, planta, polipéptido, producto primario, métodos y usos?.
EP2719280A1 (en) 2012-10-11 2014-04-16 Bayer CropScience AG Use of N-phenylethylpyrazole carboxamide derivatives or salts thereof for resistance management of phytopathogenic fungi
CA2888556C (en) 2012-10-19 2020-07-07 Bayer Cropscience Ag Method of plant growth promotion using carboxamide derivatives
CA2888559C (en) 2012-10-19 2021-03-02 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
US9801374B2 (en) 2012-10-19 2017-10-31 Bayer Cropscience Ag Active compound combinations comprising carboxamide derivatives
EP2908641B1 (en) 2012-10-19 2018-01-10 Bayer Cropscience AG Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives
EP2735231A1 (en) 2012-11-23 2014-05-28 Bayer CropScience AG Active compound combinations
BR122020019349B1 (pt) 2012-11-30 2021-05-11 Bayer Cropscience Ag composição, seu processo de preparação, método para controlar um ou mais microrganismos nocivos, semente resistente a microorganismos nocivos e seu método de tratamento
MX2015006328A (es) 2012-11-30 2015-09-07 Bayer Cropscience Ag Mezcla fungicida o pesticida binaria.
UA117819C2 (uk) 2012-11-30 2018-10-10 Байєр Кропсайєнс Акцієнгезелльшафт Подвійні пестицидні і фунгіцидні суміші
US9775351B2 (en) 2012-11-30 2017-10-03 Bayer Cropscience Ag Ternary fungicidal and pesticidal mixtures
CN104812247A (zh) 2012-11-30 2015-07-29 拜耳作物科学股份公司 二元杀真菌混合物
US20150305348A1 (en) 2012-12-03 2015-10-29 Bayer Cropscience Ag Composition comprising biological control agents
US9867377B2 (en) 2012-12-03 2018-01-16 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
CN105025721A (zh) 2012-12-03 2015-11-04 拜耳作物科学股份公司 包含生物防治剂和杀虫剂的组合物
CN105025722A (zh) 2012-12-03 2015-11-04 拜耳作物科学股份公司 包含生物防治剂和杀虫剂的组合物
EP3318129B1 (en) 2012-12-03 2019-11-06 Bayer CropScience Aktiengesellschaft Method for pest control by applying a combination of paecilomyces lilacinus and fluopyram
US20150282490A1 (en) 2012-12-03 2015-10-08 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide
WO2014086753A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising biological control agents
BR112015012785A2 (pt) 2012-12-03 2017-07-11 Bayer Cropscience Ag composição compreendendo um agente de controle biológico e um fungicida
WO2014090765A1 (en) 2012-12-12 2014-06-19 Bayer Cropscience Ag Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops
AR093996A1 (es) 2012-12-18 2015-07-01 Bayer Cropscience Ag Combinaciones bactericidas y fungicidas binarias
IN2015DN04206A (zh) 2012-12-19 2015-10-16 Bayer Cropscience Ag
EP2953466A1 (en) 2013-02-11 2015-12-16 Bayer Cropscience LP Compositions comprising gougerotin and an insecticide
AU2014214705A1 (en) 2013-02-11 2015-08-06 Bayer Cropscience Lp Compositions comprising a streptomyces-based biological control agent and another biological control agent
EP2953464A1 (en) 2013-02-11 2015-12-16 Bayer Cropscience LP Compositions comprising gougerotin and a fungicide
UA122046C2 (uk) 2013-03-07 2020-09-10 Атенікс Корп. Рекомбінантний поліпептид із інсектицидною активністю
EP2986117A1 (en) 2013-04-19 2016-02-24 Bayer CropScience Aktiengesellschaft Binary insecticidal or pesticidal mixture
BR112015026235A2 (pt) 2013-04-19 2017-10-10 Bayer Cropscience Ag método para melhorar a utilização do potencial de produção de plantas transgênicas envolvendo a aplicação de um derivado de ftaldiamida
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
TW201507722A (zh) 2013-04-30 2015-03-01 Bayer Cropscience Ag 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類
BR112015031235A2 (pt) 2013-06-26 2017-07-25 Bayer Cropscience Ag derivados de n-cicloalquil-n-[(biciclil-fenil)metileno]-(tio)carboxamida
US10070645B2 (en) 2013-12-05 2018-09-11 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-N-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
EP3077377B1 (en) 2013-12-05 2020-01-22 Bayer CropScience Aktiengesellschaft N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
EP2885970A1 (en) 2013-12-21 2015-06-24 Bayer CropScience AG Fungicide compositions comprising compound I, at least one succinate dehydrogenase (SDH) inhibitor and at least one triazole fungicide
CN106459986B (zh) 2014-03-11 2020-06-30 拜耳作物科学有限合伙公司 Hppd变体和使用方法
WO2015160619A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a fungicide
WO2015160618A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a biological control agent
WO2015160620A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and an insecticide
US10214510B2 (en) 2015-04-13 2019-02-26 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-N-(biheterocyclylethylene)-(thio)carboxamide derivatives
EP3097782A1 (en) 2015-05-29 2016-11-30 Bayer CropScience Aktiengesellschaft Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents
UA126550C2 (uk) 2015-09-11 2022-11-02 Байєр Кропсайєнс Акцієнгезелльшафт Рекомбінантна молекула нуклеїнової кислоти, що кодує гфпд, і спосіб її застосування
WO2018019676A1 (en) 2016-07-29 2018-02-01 Bayer Cropscience Aktiengesellschaft Active compound combinations and methods to protect the propagation material of plants
CN110267975B (zh) 2016-11-23 2024-04-19 巴斯夫农业种子解决方案美国有限责任公司 Axmi669和axmi991毒素基因及其使用方法
WO2018114393A1 (en) 2016-12-19 2018-06-28 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
AU2017382333B2 (en) 2016-12-22 2024-02-15 BASF Agricultural Solutions Seed US LLC Elite event EE-GM5 and methods and kits for identifying such event in biological samples
EP3559240A1 (en) 2016-12-22 2019-10-30 Basf Agricultural Solutions Seed Us Llc Elite event ee-gm4 and methods and kits for identifying such event in biological samples
BR112019012897A2 (pt) 2016-12-22 2020-01-07 BASF Agricultural Solutions Seed US LLC Métodos de controle de população de pragas, de proteção de plantas contra pragas e de aumento da produção em plantas
GB201622007D0 (en) 2016-12-22 2017-02-08 And See Cambridge Display Tech Ltd Syngenta Participations Ag Polymorphs
DK3558961T3 (da) 2016-12-22 2021-04-12 Syngenta Participations Ag Polymorfer
AR110756A1 (es) 2017-01-18 2019-05-02 Bayer Cropscience Lp Uso de bp005 para el control de patógenos de planta
CA3049775A1 (en) 2017-01-18 2018-07-26 BASF Agricultural Solutions Seed US LLC Bp005 toxin gene and methods for its use
EP3585773B1 (en) 2017-02-21 2021-04-07 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
UY37623A (es) 2017-03-03 2018-09-28 Syngenta Participations Ag Derivados de oxadiazol tiofeno fungicidas
CA3055317A1 (en) 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
BR112019020134B1 (pt) 2017-03-31 2023-05-09 Syngenta Participations Ag Composições fungicidas
CN110461159B (zh) 2017-03-31 2022-03-11 先正达参股股份有限公司 杀真菌组合物
EP3606913B1 (en) 2017-04-03 2022-04-27 Syngenta Participations AG Microbiocidal oxadiazole derivatives
WO2018184988A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
BR112019020756B1 (pt) 2017-04-05 2023-11-28 Syngenta Participations Ag Compostos derivados de oxadiazol microbicidas, composição agroquímica compreendendo os mesmos, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos e uso desses compostos
WO2018184987A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
BR112019020739B1 (pt) 2017-04-05 2023-12-19 Syngenta Participations Ag Compostos derivados de oxadiazol microbiocidas e seu uso, composição agroquímica, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos
WO2018184986A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2018184982A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2018185211A1 (en) 2017-04-06 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
EP3606912A1 (en) 2017-04-07 2020-02-12 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018188962A1 (en) 2017-04-11 2018-10-18 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
US20200163332A1 (en) 2017-04-21 2020-05-28 Bayer Cropscience Lp Method of improving crop safety
WO2018202491A1 (en) 2017-05-04 2018-11-08 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2018202487A1 (en) 2017-05-04 2018-11-08 Basf Se Substituted 5-(haloalkyl)-5-hydroxy-isoxazoles for combating phytopathogenic fungi
BR112019024993A2 (pt) 2017-06-02 2020-06-16 Syngenta Participations Ag Composições fungicidas
WO2018219797A1 (en) 2017-06-02 2018-12-06 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
EP3630753A1 (en) 2017-06-02 2020-04-08 Syngenta Participations AG Microbiocidal oxadiazole derivatives
CN110740644B (zh) 2017-06-14 2021-09-28 先正达参股股份有限公司 杀真菌组合物
US20200190043A1 (en) 2017-06-19 2020-06-18 Basf Se 2-[[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]aryloxy](thio)acetamides for combating phytopathogenic fungi
US20200315176A1 (en) 2017-06-28 2020-10-08 Syngenta Participations Ag Fungicidal compositions
BR112020000465B1 (pt) 2017-07-11 2024-02-20 Syngenta Participations Ag Derivados oxadiazol microbiocidas
BR112020000470A2 (pt) 2017-07-11 2020-07-21 Syngenta Participations Ag derivados oxadiazol microbiocidas
BR112020000456A2 (pt) 2017-07-11 2020-07-21 Syngenta Participations Ag derivados oxadiazol microbiocidas
BR112020000457A2 (pt) 2017-07-11 2020-07-21 Syngenta Participations Ag derivados oxadiazol microbiocidas
WO2019012011A1 (en) 2017-07-12 2019-01-17 Syngenta Participations Ag MICROBIOCIDE OXADIAZOLE DERIVATIVES
BR112020000414A2 (pt) 2017-07-12 2020-07-21 Syngenta Participations Ag derivados de oxadiazol microbicidas
WO2019012003A1 (en) 2017-07-13 2019-01-17 Syngenta Participations Ag MICROBIOCIDE OXADIAZOLE DERIVATIVES
MX2020000971A (es) 2017-07-27 2020-07-13 Basf Se Uso de composiciones herbicidas a base de l-glufosinato en cultivos de campo tolerantes.
WO2019025250A1 (en) 2017-08-04 2019-02-07 Basf Se SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR COMBATING PHYTOPATHOGENIC FUNGI
WO2019038042A1 (en) 2017-08-21 2019-02-28 Basf Se SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR THE CONTROL OF PHYTOPATHOGENIC FUNGI
WO2019052932A1 (en) 2017-09-18 2019-03-21 Basf Se SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR COMBATING PHYTOPATHOGENIC FUNGI
UY37913A (es) 2017-10-05 2019-05-31 Syngenta Participations Ag Derivados de picolinamida fungicidas que portan un grupo terminal cuaternario
UY37912A (es) 2017-10-05 2019-05-31 Syngenta Participations Ag Derivados de picolinamida fungicidas que portan grupos terminales heteroarilo o heteroariloxi
WO2019068811A1 (en) 2017-10-06 2019-04-11 Bayer Aktiengesellschaft COMPOSITIONS COMPRISING FLUOPYRAM AND TIOXAZAFENE
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS
US11279944B2 (en) 2017-10-24 2022-03-22 BASF Agricultural Solutions Seed US LLC Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean
CN111344279B (zh) 2017-11-15 2023-07-07 先正达参股股份有限公司 杀微生物的吡啶酰胺衍生物
EP3713936B1 (en) 2017-11-23 2021-10-20 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
BR112020010927A2 (pt) 2017-11-30 2020-12-01 Boragen, Inc. compostos de benzoxaborol e formulações dos mesmos
WO2019121143A1 (en) 2017-12-20 2019-06-27 Basf Se Substituted cyclopropyl derivatives
WO2019137995A1 (en) 2018-01-11 2019-07-18 Basf Se Novel pyridazine compounds for controlling invertebrate pests
US20200383333A1 (en) 2018-01-29 2020-12-10 BASF Agro B.V. New agrochemical formulations
JP2021512887A (ja) 2018-02-07 2021-05-20 ビーエイエスエフ・ソシエタス・エウロパエアＢａｓｆ Ｓｅ 新規ピリジンカルボキサミド
WO2019154665A1 (en) 2018-02-07 2019-08-15 Basf Se New pyridine carboxamides
WO2019166257A1 (en) 2018-03-01 2019-09-06 BASF Agro B.V. Fungicidal compositions of mefentrifluconazole
WO2019207062A1 (en) 2018-04-26 2019-10-31 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2019219464A1 (en) 2018-05-15 2019-11-21 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019224092A1 (en) 2018-05-22 2019-11-28 Basf Se Pesticidally active c15-derivatives of ginkgolides
JP2021525774A (ja) 2018-06-04 2021-09-27 バイエル アクチェンゲゼルシャフトＢａｙｅｒ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 除草活性二環式ベンゾイルピラゾール
BR112020026877A2 (pt) 2018-06-29 2021-04-06 Syngenta Crop Protection Ag Derivados de oxadiazol microbiocidas
WO2020007658A1 (en) 2018-07-02 2020-01-09 Syngenta Crop Protection Ag 3-(2-thienyl)-5-(trifluoromethyl)-1,2,4-oxadiazole derivatives as agrochemical fungicides
WO2020016180A1 (en) 2018-07-16 2020-01-23 Syngenta Crop Protection Ag Microbiocidal oxadiazole derivatives
US10499599B1 (en) 2018-08-06 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 78020134
US10455797B1 (en) 2018-08-06 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar S170108
US10440929B1 (en) 2018-08-06 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar S170077
US10548272B1 (en) 2018-08-06 2020-02-04 M.S. Technologies, L.L.C. Soybean cultivar S170070
US10499600B1 (en) 2018-08-06 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar S170068
US10588283B2 (en) 2018-08-06 2020-03-17 M.S. Technologies, L.L.C. Soybean cultivar S170076
US10542695B1 (en) 2018-08-06 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 75163252
US10506785B1 (en) 2018-08-06 2019-12-17 M.S. Technologies, L.L.C. Soybean cultivar S170122
US10542697B1 (en) 2018-08-06 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar S170114
US10542696B1 (en) 2018-08-06 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar S170106
US10455798B1 (en) 2018-08-06 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar S170052
US10390513B1 (en) 2018-08-07 2019-08-27 M.S. Technologies, L.L.C. Soybean cultivar S170107
US10687488B2 (en) 2018-08-07 2020-06-23 M.S. Technologies, L.L.C. Soybean cultivar S170041
US10412923B1 (en) 2018-08-07 2019-09-17 M.S. Technologies, L.L.C. Soybean cultivar S170047
US10368524B1 (en) 2018-08-07 2019-08-06 M.S. Technologies, L.L.C. Soybean cultivar S170057
US10542718B1 (en) 2018-08-07 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar S170042
US10631498B2 (en) 2018-08-07 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar S170111
US10798906B2 (en) 2018-08-07 2020-10-13 M.S. Technologies, L.L.C. Soybean cultivar S170112
US10375921B1 (en) 2018-08-07 2019-08-13 M.S. Technologies, L.L.C. Soybean cultivar S170053
US10548281B1 (en) 2018-08-07 2020-02-04 M.S. Technologies, L.L.C. Soybean cultivar S170061
US10375920B1 (en) 2018-08-07 2019-08-13 M.S. Technologies, L.L.C. Soybean cultivar S170117
US10548280B1 (en) 2018-08-07 2020-02-04 M.S. Technologies, L.L.C. Soybean cultivar S170059
US10555471B1 (en) 2018-08-07 2020-02-11 M.S. Technologies, L.L.C. Soybean cultivar S170082
US10595485B2 (en) 2018-08-07 2020-03-24 M.S. Technologies, L.L.C. Soybean cultivar S170044
US10687489B2 (en) 2018-08-07 2020-06-23 M.S. Technologies, L.L.C. Soybean cultivar S170065
US10542717B1 (en) 2018-08-07 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar S170038
US10512230B1 (en) 2018-08-07 2019-12-24 M.S. Technologies, L.L.C. Soybean cultivar S170055
US10368522B1 (en) 2018-08-07 2019-08-06 M.S. Technologies, L.L.C. Soybean cultivar S170118
US10455800B1 (en) 2018-08-07 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar S170109
US10531623B1 (en) 2018-08-07 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar S170079
US10492449B1 (en) 2018-08-07 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar S170048
US10412922B1 (en) 2018-08-07 2019-09-17 M.S. Technologies, L.L.C. Soybean cultivar S170113
US10455799B1 (en) 2018-08-07 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar S170074
US10368523B1 (en) 2018-08-07 2019-08-06 M.S. Technologies, L.L.C. Soybean cultivar S170119
US10477820B1 (en) 2018-08-07 2019-11-19 M.S. Technologies, L.L.C. Soybean cultivar S170120
US10492447B1 (en) 2018-08-07 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar S170105
US10433508B1 (en) 2018-08-07 2019-10-08 M.S. Technologies, L.L.C. Soybean cultivar S170110
US10492446B1 (en) 2018-08-07 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar S170067
US10531622B1 (en) 2018-08-07 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar S170072
US10687486B2 (en) 2018-08-07 2020-06-23 M.S. Technologies, L.L.C. Soybean cultivar S170116
US10433520B1 (en) 2018-08-07 2019-10-08 M.S. Technologies, L.L.C. Soybean cultivar S170049
US10687487B2 (en) 2018-08-07 2020-06-23 M.S. Technologies, L.L.C. Soybean cultivar S170121
US10492448B1 (en) 2018-08-07 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar S170104
US10531624B1 (en) 2018-08-07 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar S170115
US10602688B2 (en) 2018-08-07 2020-03-31 M.S. Technologies, L.L.C. Soybean cultivar S170062
MX2021001904A (es) 2018-08-18 2021-05-27 Boragen Inc Formas solidas de benzoxaborol sustituido y composiciones de las mismas.
US10973198B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072340
US10897868B2 (en) 2018-08-22 2021-01-26 Monsanto Technology Llc Soybean variety 01072327
US11109552B2 (en) 2018-08-22 2021-09-07 Monsanto Technology Llc Soybean variety 01072332
US10905081B2 (en) 2018-08-22 2021-02-02 Monsanto Technology Llc Soybean variety 01072329
US11206791B2 (en) 2018-08-22 2021-12-28 Monsanto Technology Llc Soybean variety 01072337
US11013197B2 (en) 2018-08-22 2021-05-25 Monsanto Technology Llc Soybean variety 01072368
US10897869B2 (en) 2018-08-22 2021-01-26 Monsanto Technology Llc Soybean variety 01072333
US10973201B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072339
US11096363B2 (en) 2018-08-22 2021-08-24 Monsanto Technology Llc Soybean variety 01072331
EP3613736A1 (en) 2018-08-22 2020-02-26 Basf Se Substituted glutarimide derivatives
US11109553B2 (en) 2018-08-22 2021-09-07 Monsanto Technology Llc Soybean variety 01072328
US10905080B2 (en) 2018-08-22 2021-02-02 Monsanto Technology Llc Soybean variety 01072355
US10939652B2 (en) 2018-08-22 2021-03-09 Monsanto Technology Llc Soybean variety 01072350
US10999998B2 (en) 2018-08-22 2021-05-11 Monsanto Technology Llc Soybean variety 01072346
US11026389B2 (en) 2018-08-22 2021-06-08 Monsanto Technology Llc Soybean variety 01072367
US10973199B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072341
US10918065B2 (en) 2018-08-22 2021-02-16 Monsanto Technology Llc Soybean variety 01072343
US10973200B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072342
US10993404B2 (en) 2018-08-22 2021-05-04 Monsanto Technology Llc Soybean variety 01072356
US10897870B2 (en) 2018-08-22 2021-01-26 Monsanto Technology Llc Soybean variety 01072322
US11044876B2 (en) 2018-08-22 2021-06-29 Monsanto Technology Llc Soybean variety 01072345
EP3628158A1 (en) 2018-09-28 2020-04-01 Basf Se Pesticidal mixture comprising a mesoionic compound and a biopesticide
CN113195462A (zh) 2018-10-17 2021-07-30 先正达农作物保护股份公司 杀微生物的噁二唑衍生物
AR116628A1 (es) 2018-10-18 2021-05-26 Syngenta Crop Protection Ag Compuestos microbiocidas
US20210347777A1 (en) 2018-10-23 2021-11-11 Basf Se Tricyclic pesticidal compounds
EP3643705A1 (en) 2018-10-24 2020-04-29 Basf Se Pesticidal compounds
EP3670501A1 (en) 2018-12-17 2020-06-24 Basf Se Substituted [1,2,4]triazole compounds as fungicides
FI3908584T3 (fi) 2019-01-11 2023-06-19 Basf Se 1-(1,2-dimetyylipropyyli)-N-etyyli-5-metyyli-N-pyridatsin-4-yylipyratsoli-4-karboksamidin kiteisiä muotoja
EP3696177A1 (en) 2019-02-12 2020-08-19 Basf Se Heterocyclic compounds for the control of invertebrate pests
WO2020165403A1 (en) 2019-02-15 2020-08-20 Syngenta Crop Protection Ag Phenyl substituted thiazole derivatives as microbiocidal compounds
WO2020169651A1 (en) 2019-02-20 2020-08-27 Syngenta Crop Protection Ag Use of spiropidion
GB201903942D0 (en) 2019-03-22 2019-05-08 Syngenta Crop Protection Ag Microbiocidal compounds
WO2020208095A1 (en) 2019-04-10 2020-10-15 Syngenta Crop Protection Ag Microbiocidal picolinamide derivatives
EP3952652A1 (en) 2019-04-10 2022-02-16 Syngenta Crop Protection AG Fungicidal compositions
US20220240508A1 (en) 2019-05-10 2022-08-04 Bayer Cropscience Lp Active compound combinations
WO2020239517A1 (en) 2019-05-29 2020-12-03 Basf Se Mesoionic imidazolium compounds and derivatives for combating animal pests
EP3769623A1 (en) 2019-07-22 2021-01-27 Basf Se Mesoionic imidazolium compounds and derivatives for combating animal pests
WO2020244970A1 (en) 2019-06-06 2020-12-10 Basf Se New carbocyclic pyridine carboxamides
CR20210605A (es) 2019-06-06 2022-01-07 Basf Se N-(prid-3-il)carboxamidas fungicidas
WO2020244969A1 (en) 2019-06-06 2020-12-10 Basf Se Pyridine derivatives and their use as fungicides
US10694695B1 (en) 2019-06-19 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 84420047
US10609884B1 (en) 2019-06-19 2020-04-07 M.S. Technologies, L.L.C. Soybean cultivar 85380644
US10631490B1 (en) 2019-06-19 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 82410316
US10694705B1 (en) 2019-06-19 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 83410047
US10765082B1 (en) 2019-06-19 2020-09-08 M.S. Technologies, L.L.C. Soybean cultivar 83261548
US10765077B1 (en) 2019-06-19 2020-09-08 M.S. Technologies, L.L.C. Soybean cultivar 81142227
US10631489B1 (en) 2019-06-19 2020-04-28 M.S. Technologyies, L.L.C. Soybean cultivar 80142922
US10757878B1 (en) 2019-06-19 2020-09-01 M.S. Technologies, L.L.C. Soybean cultivar 85162129
US10631488B1 (en) 2019-06-19 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 86172129
US10694710B1 (en) 2019-06-20 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 81290801
US10869451B1 (en) 2019-06-20 2020-12-22 M.S. Technologies, L.L.C. Soybean cultivar 89081933
US10694709B1 (en) 2019-06-20 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 80260801
US10694707B1 (en) 2019-06-20 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 80361712
US10863687B1 (en) 2019-06-20 2020-12-15 M.S. Technologies, L.L.C. Soybean cultivar 80191933
US10842115B1 (en) 2019-06-20 2020-11-24 M.S. Technologies, L.L.C. Soybean cultivar 83090832
US10694706B1 (en) 2019-06-20 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 82092934
US10869450B1 (en) 2019-06-20 2020-12-22 M.S. Technologies, L.L.C. Soybean cultivar 86372839
US10743506B1 (en) 2019-06-20 2020-08-18 M.S. Technologies, L.L.C. Soybean cultivar 85321341
US10869439B1 (en) 2019-06-20 2020-12-22 M.S. Technologies, L.L.C. Soybean cultivar 84080909
US10694711B1 (en) 2019-06-20 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 82201801
US10863686B1 (en) 2019-06-20 2020-12-15 M.S. Technologies, L.L.C. Soybean cultivar 88061933
US10694708B1 (en) 2019-06-20 2020-06-30 M.S. Technologies, L.L.C. Soybean cultivar 81162507
US10785931B1 (en) 2019-06-20 2020-09-29 M.S. Technologies, L.L.C. Soybean cultivar 83070909
BR112021026861A2 (pt) 2019-07-05 2022-02-22 Syngenta Crop Protection Ag Derivados de picolinamida microbiocidas
GB201910037D0 (en) 2019-07-12 2019-08-28 Syngenta Crop Protection Ag Microbiocidal compounds
EP3766879A1 (en) 2019-07-19 2021-01-20 Basf Se Pesticidal pyrazole derivatives
JP2022541612A (ja) 2019-07-22 2022-09-26 バイエル、アクチエンゲゼルシャフト 殺虫剤としての５－アミノ置換ピラゾールおよびトリアゾール
KR20220038402A (ko) 2019-07-23 2022-03-28 바이엘 악티엔게젤샤프트 살충제로서의 신규 헤테로아릴-트리아졸 화합물
UY38796A (es) 2019-07-23 2021-02-26 Bayer Ag Novedosos compuestos de heteroaril-triazol como plaguicidas
EP4007494A1 (en) 2019-08-01 2022-06-08 Bayer CropScience LP Method of improving cold stress tolerance and crop safety
US11116164B2 (en) 2019-08-06 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 85260926
US10897865B1 (en) 2019-08-06 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 85021070
US11058087B2 (en) 2019-08-06 2021-07-13 M.S. Technologies, L.L.C. Soybean cultivar 84110426
US11083158B2 (en) 2019-08-06 2021-08-10 M.S. Technologies, L.L.C. Soybean cultivar 80361023
US10980202B2 (en) 2019-08-06 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 83162926
US11044864B2 (en) 2019-08-06 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 84370213
US10918057B1 (en) 2019-08-06 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 89370134
US10918059B1 (en) 2019-08-06 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 83303830
US10905076B1 (en) 2019-08-06 2021-02-02 M.S. Technologies, L.L.C. Soybean cultivar 87463802
US11140844B2 (en) 2019-08-06 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 87140321
US10918058B1 (en) 2019-08-06 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 81092033
US11058088B2 (en) 2019-08-06 2021-07-13 M.S. Technologies, L.L.C. Soybean cultivar 85120426
US10980201B2 (en) 2019-08-06 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 87370220
US10925242B1 (en) 2019-08-07 2021-02-23 M.S. Technologies, L.L.C. Soybean cultivar 88161514
US10918062B1 (en) 2019-08-07 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 88001207
US10918063B1 (en) 2019-08-07 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 82230226
US10925243B1 (en) 2019-08-07 2021-02-23 M.S. Technologies, L.L.C. Soybean cultivar 87112125
US10918041B1 (en) 2019-08-07 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 81252901
US11096358B2 (en) 2019-08-07 2021-08-24 M.S. Technologies, L.L.C. Soybean cultivar 88340813
US10881075B1 (en) 2019-08-07 2021-01-05 M.S. Technologies, L.L.C. Soybean cultivar 86111722
US10918061B1 (en) 2019-08-07 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 82161323
US10905077B1 (en) 2019-08-07 2021-02-02 M.S. Technologies, L.L.C. Soybean cultivar 80560821
US10912272B1 (en) 2019-08-07 2021-02-09 M.S. Technologies, L.L.C. Soybean cultivar 81382112
US10918060B1 (en) 2019-08-07 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 81171313
US10939650B2 (en) 2019-08-08 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 82072600
US10939649B2 (en) 2019-08-08 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 84372449
US10905078B1 (en) 2019-08-08 2021-02-02 M.S. Technologies, L.L.C. Soybean cultivar 85161246
EP3701796A1 (en) 2019-08-08 2020-09-02 Bayer AG Active compound combinations
US10912275B1 (en) 2019-08-08 2021-02-09 M.S. Technologies, L.L.C. Soybean cultivar 89242728
US10939634B2 (en) 2019-08-08 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 83082600
US10912274B1 (en) 2019-08-08 2021-02-09 M.S. Technologies, L.L.C. Soybean cultivar 86171246
US11076553B2 (en) 2019-08-08 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 86270804
US10925244B1 (en) 2019-08-08 2021-02-23 M.S. Technologies, L.L.C. Soybean cultivar 88212722
US10842118B1 (en) 2019-08-08 2020-11-24 M.S. Technologies, L.L.C. Soybean cultivar 84252433
US11185043B2 (en) 2019-09-16 2021-11-30 Monsanto Technology Llc Soybean variety 01077334
US11259494B2 (en) 2019-09-16 2022-03-01 Monsanto Technology Llc Soybean variety 01077345
US11178841B2 (en) 2019-09-16 2021-11-23 Monsanto Technology Llc Soybean variety 01077386
US11266105B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01077338
US11266106B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01081407
US11284587B2 (en) 2019-09-16 2022-03-29 Monsanto Technology Llc Soybean variety 01077381
US11337400B2 (en) 2019-09-16 2022-05-24 Monsanto Technology, Llc Soybean variety 01077373
US11185044B2 (en) 2019-09-16 2021-11-30 Monsanto Technology Llc Soybean variety 01077343
US11266110B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01077337
US11266109B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01077385
US11229182B2 (en) 2019-09-16 2022-01-25 Monsanto Technology Llc Soybean variety 01077387
US20210076615A1 (en) * 2019-09-16 2021-03-18 Monsanto Technology Llc Soybean variety 01077333
US11350595B2 (en) 2019-09-16 2022-06-07 Monsanto Technology, Llc Soybean variety 01077372
US11259493B2 (en) 2019-09-16 2022-03-01 Monsanto Technology Llc Soybean variety 01077341
US11266107B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01081408
US11576340B2 (en) * 2019-09-17 2023-02-14 Monsanto Technology, Llc Soybean variety 01081414
US11297792B2 (en) 2019-09-17 2022-04-12 Monsanto Technology Llc Soybean variety 01077344
US11576341B2 (en) * 2019-09-17 2023-02-14 Monsanto Technology, Llc Soybean variety 01072361
US11266111B2 (en) 2019-09-17 2022-03-08 Monsanto Technology Llc Soybean variety 01077383
US11266108B2 (en) 2019-09-17 2022-03-08 Monsanto Technology Llc Soybean variety 01081409
US20220403410A1 (en) 2019-09-26 2022-12-22 Bayer Aktiengesellschaft Rnai-mediated pest control
US20220361492A1 (en) 2019-10-02 2022-11-17 Bayer Aktiengesellschaft Active compound combinations comprising fatty acids
WO2021063736A1 (en) 2019-10-02 2021-04-08 Basf Se Bicyclic pyridine derivatives
WO2021063735A1 (en) 2019-10-02 2021-04-08 Basf Se New bicyclic pyridine derivatives
JP2022551309A (ja) 2019-10-09 2022-12-08 バイエル・アクチエンゲゼルシヤフト 殺有害生物剤としての新規ヘテロアリール－トリアゾール化合物
CN114728928A (zh) 2019-10-09 2022-07-08 拜耳公司 作为农药的新的杂芳基三唑化合物
US11324181B2 (en) 2019-10-18 2022-05-10 Monsanto Technology Llc Soybean variety 01077335
US11272679B2 (en) 2019-10-18 2022-03-15 Monsanto Technology Llc Soybean variety 01077330
US11252921B2 (en) 2019-10-18 2022-02-22 Monsanto Technology Llc Soybean variety 01077393
US11252922B2 (en) 2019-10-18 2022-02-22 Monsanto Technology Llc Soybean variety 01077391
US11330783B2 (en) 2019-10-18 2022-05-17 Monsanto Technology, Llc Soybean variety 01077336
US11330777B2 (en) 2019-10-18 2022-05-17 Monsanto Technology, Llc Soybean variety 01077390
EP4055010A1 (de) 2019-11-07 2022-09-14 Bayer Aktiengesellschaft Substituierte sulfonylamide zur bekämpfung tierischer schädlinge
WO2021097162A1 (en) 2019-11-13 2021-05-20 Bayer Cropscience Lp Beneficial combinations with paenibacillus
EP4061131A1 (en) 2019-11-18 2022-09-28 Bayer Aktiengesellschaft Active compound combinations comprising fatty acids
TW202134226A (zh) 2019-11-18 2021-09-16 德商拜耳廠股份有限公司 作為殺蟲劑之新穎雜芳基-三唑化合物
TW202136248A (zh) 2019-11-25 2021-10-01 德商拜耳廠股份有限公司 作為殺蟲劑之新穎雜芳基-三唑化合物
BR112022012417A2 (pt) 2020-01-31 2022-08-30 Pairwise Plants Services Inc Supressão da resposta de evitação da sombra nas plantas
BR112022016400A2 (pt) 2020-02-18 2022-10-25 Bayer Ag Compostos inovadores de heteroaril-triazol como pesticidas
EP3708565A1 (en) 2020-03-04 2020-09-16 Bayer AG Pyrimidinyloxyphenylamidines and the use thereof as fungicides
UY39115A (es) 2020-03-05 2021-10-29 Syngenta Crop Protection Ag Mezclas fungicidas de derivados de arilo metoxiacrilato
EP4114184A1 (en) 2020-03-05 2023-01-11 Syngenta Crop Protection AG Fungicidal compositions
US20230212600A1 (en) 2020-04-16 2023-07-06 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021209490A1 (en) 2020-04-16 2021-10-21 Bayer Aktiengesellschaft Cyclaminephenylaminoquinolines as fungicides
KR20230007398A (ko) 2020-04-21 2023-01-12 바이엘 악티엔게젤샤프트 해충 방제제로서 2-(헤트)아릴-치환된 축합 헤테로시클릭 유도체
EP3903584A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iv
EP3903583A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iii
BR112022021631A2 (pt) 2020-04-28 2022-12-06 Basf Se Compostos, composição, métodos para combater ou controlar pragas invertebradas, para proteger plantas em crescimento e para tratar ou proteger um animal, semente e uso de um composto
EP3903582A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ii
EP3903581A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i
GB202006386D0 (en) 2020-04-30 2020-06-17 Syngenta Crop Protection Ag Microbiocidal Compounds
GB202006399D0 (en) 2020-04-30 2020-06-17 Syngenta Crop Protection Ag Microbiocidal compounds
GB202006480D0 (en) 2020-05-01 2020-06-17 Syngenta Crop Protection Ag Microbiocidal compounds
GB202006606D0 (en) 2020-05-05 2020-06-17 Syngenta Crop Protection Ag Microbiocidal compounds
TW202208347A (zh) 2020-05-06 2022-03-01 德商拜耳廠股份有限公司 作為殺蟲劑之新穎雜芳基三唑化合物
US20230348392A1 (en) 2020-05-06 2023-11-02 Bayer Aktiengesellschaft Pyridine (thio)amides as fungicidal compounds
US20230180756A1 (en) 2020-05-12 2023-06-15 Bayer Aktiengesellschaft Triazine and pyrimidine (thio)amides as fungicidal compounds
EP3909950A1 (en) 2020-05-13 2021-11-17 Basf Se Heterocyclic compounds for the control of invertebrate pests
EP4153566A1 (en) 2020-05-19 2023-03-29 Bayer CropScience Aktiengesellschaft Azabicyclic(thio)amides as fungicidal compounds
CN116096230A (zh) 2020-06-02 2023-05-09 成对植物服务股份有限公司 控制分生组织大小以改良作物的方法
BR112022024394A2 (pt) 2020-06-04 2023-01-31 Bayer Ag Heterociclil pirimidinas e triazinas como novos fungicidas
CN116057056A (zh) 2020-06-10 2023-05-02 拜耳公司 作为杀真菌剂的氮杂双环取代的杂环化合物
EP3945089A1 (en) 2020-07-31 2022-02-02 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors v
WO2021249800A1 (en) 2020-06-10 2021-12-16 Basf Se Substituted [1,2,4]triazole compounds as fungicides
MX2022015896A (es) 2020-06-17 2023-02-22 Pairwise Plants Services Inc Métodos para el control del tamaño del meristemo para la mejora de cultivos.
EP4167738A1 (en) 2020-06-18 2023-04-26 Bayer Aktiengesellschaft Composition for use in agriculture
WO2021255071A1 (en) 2020-06-18 2021-12-23 Bayer Aktiengesellschaft 3-(pyridazin-4-yl)-5,6-dihydro-4h-1,2,4-oxadiazine derivatives as fungicides for crop protection
WO2021255089A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines and 1,3,4-oxadiazole pyridines as fungicides
WO2021255091A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazoles and their derivatives as fungicides
UY39275A (es) 2020-06-19 2022-01-31 Bayer Ag 1,3,4-oxadiazol pirimidinas como fungicidas, procesos e intermediarios para su preparación, métodos de uso y usos de los mismos
UY39276A (es) 2020-06-19 2022-01-31 Bayer Ag Uso de compuestos de 1,3,4–oxadiazol–2–ilpirimidina para controlar microorganismos fitopatógenos, métodos de uso y composiciones.
EP3929189A1 (en) 2020-06-25 2021-12-29 Bayer Animal Health GmbH Novel heteroaryl-substituted pyrazine derivatives as pesticides
KR20230039665A (ko) 2020-07-02 2023-03-21 바이엘 악티엔게젤샤프트 해충 방제제로서의 헤테로사이클 유도체
EP3939961A1 (en) 2020-07-16 2022-01-19 Basf Se Strobilurin type compounds and their use for combating phytopathogenic fungi
WO2022017836A1 (en) 2020-07-20 2022-01-27 BASF Agro B.V. Fungicidal compositions comprising (r)-2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1- (1,2,4-triazol-1-yl)propan-2-ol
EP3970494A1 (en) 2020-09-21 2022-03-23 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors viii
WO2022033991A1 (de) 2020-08-13 2022-02-17 Bayer Aktiengesellschaft 5-amino substituierte triazole als schädlingsbekämpfungsmittel
US11337399B2 (en) 2020-08-18 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 98250971
US11425878B2 (en) 2020-08-18 2022-08-30 M.S. Technologies, L.L.C. Soybean cultivar 96140971
US11229181B1 (en) 2020-08-18 2022-01-25 M.S. Technologies, L.L.C. Soybean cultivar 90110353
US11252920B1 (en) 2020-08-18 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 95482129
US11277994B2 (en) 2020-08-18 2022-03-22 M.S. Technologies, L.L.C. Soybean cultivar 93421515
US11185042B1 (en) 2020-08-18 2021-11-30 M.S. Technologies, L.L.C. Soybean cultivar 94020016
US11259492B1 (en) 2020-08-18 2022-03-01 M.S. Technologies, L.L.C. Soybean cultivar 94041029
US11234404B1 (en) 2020-08-18 2022-02-01 M.S. Technologies, L.L.C. Soybean cultivar 98010233
US11252916B1 (en) 2020-08-18 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 95110118
US11432510B2 (en) 2020-08-18 2022-09-06 M.S. Technologies, L.L.C. Soybean cultivar 97330971
US11229180B1 (en) 2020-08-18 2022-01-25 M.S. Technologies, L.L.C. Soybean cultivar 95230842
US11252919B1 (en) 2020-08-18 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 93040644
US11178840B1 (en) 2020-08-18 2021-11-23 M.S. Technologies, L.L.C. Soybean cultivar 95130648
US11234403B1 (en) 2020-08-18 2022-02-01 M.S. Technologies, L.L.C. Soybean cultivar 92181128
US11357199B2 (en) 2020-08-18 2022-06-14 M.S. Technologies, L.L.C. Soybean cultivar 94150027
US11252918B1 (en) 2020-08-18 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 97150620
US11337398B2 (en) 2020-08-18 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 99050353
US11252917B1 (en) 2020-08-18 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 92130046
US11178839B1 (en) 2020-08-18 2021-11-23 M.S. Technologies, L.L.C. Soybean cultivar 97250443
US11425877B2 (en) 2020-08-18 2022-08-30 M.S. Technologies, L.L.C. Soybean cultivar 95130328
KR20230058455A (ko) 2020-08-31 2023-05-03 바스프 에스이 식물 수확량 개선
US20220071132A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 99030218
US20220071144A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 92190428
US20220071133A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 90150218
WO2022053453A1 (de) 2020-09-09 2022-03-17 Bayer Aktiengesellschaft Azolcarboxamide als schädlingsbekämpfungsmittel
US20220071160A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 94411038
US20220071134A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 95460218
US20220071138A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 91132218
US11234406B1 (en) 2020-09-09 2022-02-01 Monsanto Technology Llc Soybean cultivar 98010818
US20220071157A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 99250038
US11259495B1 (en) 2020-09-09 2022-03-01 Monsanto Technology Llc Soybean cultivar 90381408
US20220071155A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 92141928
US20220071140A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 96030818
US11234405B1 (en) 2020-09-09 2022-02-01 Monsanto Technology Llc Soybean cultivar 90110208
US11252923B1 (en) 2020-09-09 2022-02-22 Monsanto Technology Llc Soybean cultivar 99003018
US20220071156A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 92412928
US20220071158A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 95470038
US20220071135A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 91080218
US20220071143A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 94260428
US20220071136A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 99002218
US20220071137A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 90112218
US20220071131A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 92110218
US20220071149A1 (en) * 2020-09-09 2022-03-10 Monsanto Technology Llc Soybean cultivar 92041528
US11206797B1 (en) 2020-09-09 2021-12-28 Monsanto Technology Llc Soybean cultivar 97320218
US11317587B2 (en) 2020-09-11 2022-05-03 Stine Seed Farm, Inc. Soybean cultivar 96030600
US11277996B1 (en) 2020-09-11 2022-03-22 Monsanto Technology Llc Soybean cultivar 97051600
US11310988B2 (en) 2020-09-11 2022-04-26 Monsanto Technology Llc Soybean cultivar 92340030
US11439111B2 (en) 2020-09-11 2022-09-13 Monsanto Technology Llc Soybean cultivar 99354100
US11357200B2 (en) 2020-09-11 2022-06-14 Monsanto Technology, Llc Soybean cultivar 92131600
US20220079073A1 (en) * 2020-09-11 2022-03-17 Monsanto Technology Llc Soybean cultivar 92111703
US11229183B1 (en) 2020-09-11 2022-01-25 Monsanto Technology Llc Soybean cultivar 93070703
US11317588B2 (en) 2020-09-11 2022-05-03 Stine Seed Farm, Inc. Soybean cultivar 97080600
US11350596B2 (en) 2020-09-11 2022-06-07 Monsanto Technology, Llc Soybean cultivar 96050600
US11330784B2 (en) 2020-09-11 2022-05-17 Monsanto Technology, Llc Soybean cultivar 95322700
US11284592B1 (en) 2020-09-11 2022-03-29 Monsanto Technology Llc Soybean cultivar 91030603
US11606926B2 (en) * 2020-09-15 2023-03-21 Monsanto Technology Llc Soybean cultivar 94030201
US11343988B2 (en) 2020-09-15 2022-05-31 Stine Seed Farm, Inc. Soybean cultivar 91041800
US11310989B2 (en) 2020-09-15 2022-04-26 Monsanto Technology Llc Soybean cultivar 94071660
US11317589B2 (en) 2020-09-15 2022-05-03 Monsanto Technology Llc Soybean cultivar 95430820
US11744211B2 (en) 2020-09-15 2023-09-05 Monsanto Technology, Llc Soybean cultivar 92310017
US11369074B2 (en) 2020-09-15 2022-06-28 Monsanto Technology Llc Soybean cultivar 99122620
US11343987B2 (en) 2020-09-15 2022-05-31 Stine Seed Farm, Inc. Soybean cultivar 90401320
US11363778B2 (en) 2020-09-15 2022-06-21 Monsanto Technology Llc Soybean cultivar 90221620
US11324183B2 (en) 2020-09-15 2022-05-10 Stine Seed Farm, Inc. Soybean cultivar 90250820
US11324184B2 (en) 2020-09-15 2022-05-10 Stine Seed Farm, Inc. Soybean cultivar 97061850
WO2022058327A1 (en) 2020-09-15 2022-03-24 Bayer Aktiengesellschaft Substituted ureas and derivatives as new antifungal agents
US11324182B2 (en) 2020-09-15 2022-05-10 Stine Seed Farm, Inc. Soybean cultivar 95060020
US11363779B2 (en) 2020-09-15 2022-06-21 Monsanto Technology Llc Soybean cultivar 90301201
US11399497B2 (en) 2020-09-15 2022-08-02 Monsanto Technology, Llc Soybean cultivar 93333110
US11350597B2 (en) 2020-09-15 2022-06-07 Monsanto Technology, Llc Soybean cultivar 94111580
US11412692B2 (en) 2020-09-15 2022-08-16 Monsanto Technology Llc Soybean cultivar 90290600
US11419299B2 (en) 2020-09-15 2022-08-23 Monsanto Technology Llc Soybean cultivar 91361201
US11357201B2 (en) 2020-09-15 2022-06-14 Monsanto Technology, Llc Soybean cultivar 94411660
US11399496B2 (en) 2020-09-15 2022-08-02 Monsanto Technology, Llc Soybean cultivar 90116100
US11425879B2 (en) 2020-09-15 2022-08-30 Monsanto Technology, Llc Soybean cultivar 93054930
US11337401B2 (en) 2020-09-15 2022-05-24 Monsanto Technology, Llc Soybean cultivar 90370640
GB202014840D0 (en) 2020-09-21 2020-11-04 Syngenta Crop Protection Ag Microbiocidal compounds
US11445686B2 (en) 2020-09-22 2022-09-20 M.S. Technologies, L.L.C. Soybean cultivar 95040446
US11382300B2 (en) 2020-09-22 2022-07-12 M.S. Technologies, L.L.C. Soybean cultivar 98020446
US11350599B2 (en) 2020-09-22 2022-06-07 M.S. Technologies, L.L.C. Soybean cultivar 93410924
US11363781B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 94220186
US11432512B2 (en) 2020-09-22 2022-09-06 M.S. Technologies, L.L.C. Soybean cultivar 90210769
US11363783B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 96050446
US11363784B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 93050528
US11425880B2 (en) 2020-09-22 2022-08-30 M.S. Technologies, L.L.C. Soybean cultivar 98020803
US11363782B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 98310446
US11350598B2 (en) 2020-09-22 2022-06-07 M.S. Technologies, L.L.C. Soybean cultivar 97220468
US11357202B2 (en) 2020-09-22 2022-06-14 M.S. Technologies, L.L.C. Soybean cultivar 92440084
US11363788B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C Soybean cultivar 94120010
US11357204B2 (en) 2020-09-22 2022-06-14 M.S. Technologies, L.L.C. Soybean cultivar 98110801
US11363786B2 (en) 2020-09-22 2022-06-21 M. S. Technologies, L.L.C. Soybean cultivar 98220823
US11445687B2 (en) 2020-09-22 2022-09-20 M.S. Technologies, L.L.C. Soybean cultivar 98240804
US11439112B2 (en) 2020-09-22 2022-09-13 M.S. Technologies, L.L.C. Soybean cultivar 91110044
US11363785B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C Soybean cultivar 94040525
US11357203B2 (en) 2020-09-22 2022-06-14 M.S. Technologies, L.L.C. Soybean cultivar 93250796
US11363787B2 (en) 2020-09-22 2022-06-21 M.S. Technologies, L.L.C Soybean cultivar 99250985
US11432511B2 (en) 2020-09-22 2022-09-06 M.S. Technologies, L.L.C. Soybean cultivar 91410186
EP3974414A1 (de) 2020-09-25 2022-03-30 Bayer AG 5-amino substituierte pyrazole und triazole als schädlingsbekämpfungsmittel
CN116209355A (zh) 2020-10-27 2023-06-02 巴斯夫农业公司 包含氯氟醚菌唑的组合物
WO2022090071A1 (en) 2020-11-02 2022-05-05 Basf Se Use of mefenpyr-diethyl for controlling phytopathogenic fungi
WO2022090069A1 (en) 2020-11-02 2022-05-05 Basf Se Compositions comprising mefenpyr-diethyl
US11477960B2 (en) 2020-11-02 2022-10-25 Monsanto Technology Llc Soybean variety 01077912
US11612128B2 (en) 2020-11-02 2023-03-28 Monsanto Technology Llc Soybean variety 01083666
US11547075B2 (en) 2020-11-02 2023-01-10 Monsanto Technology Llc Soybean variety 01078822
US11510382B2 (en) 2020-11-02 2022-11-29 Monsanto Technology Llc Soybean variety 01078845
US11350600B2 (en) 2020-11-05 2022-06-07 Monsanto Technology, Llc Soybean variety 01083699
US11343993B2 (en) 2020-11-05 2022-05-31 Monsanto Technology, Llc Soybean variety 01083650
US11357205B2 (en) 2020-11-05 2022-06-14 Monsanto Technology Llc Soybean variety 01083631
US11284593B1 (en) 2020-11-05 2022-03-29 Monsanto Technology Llc Soybean variety 01083687
US11464195B2 (en) 2020-11-06 2022-10-11 Monsanto Technology Llc Soybean variety 01083654
US11528879B2 (en) 2020-11-06 2022-12-20 Monsanto Technology, Llc Soybean variety 01083659
US11445690B2 (en) 2020-11-06 2022-09-20 Monsanto Technology Llc Soybean variety 01083639
US11343995B1 (en) 2020-11-06 2022-05-31 Monsanto Technology, Llc Soybean variety 01083647
US11457601B2 (en) 2020-11-06 2022-10-04 Monsanto Technology Llc Soybean variety 01083638
US11533884B2 (en) 2020-11-06 2022-12-27 Monsanto Technology, Llc Soybean variety 01083660
US11547077B2 (en) 2020-11-06 2023-01-10 Monsanto Technology, Llc Soybean variety 01083688
US11484000B2 (en) 2020-11-06 2022-11-01 Monsanto Technology, Llc Soybean variety 01083661
US11457600B2 (en) 2020-11-06 2022-10-04 Monsanto Technology, Llc Soybean variety 01083646
US11553671B2 (en) 2020-11-06 2023-01-17 Monsanto Technology, Llc Soybean variety 01083653
US11343994B1 (en) 2020-11-06 2022-05-31 Monsanto Technology, Llc Soybean variety 01083655
WO2022106304A1 (en) 2020-11-23 2022-05-27 BASF Agro B.V. Compositions comprising mefentrifluconazole
AR124281A1 (es) 2020-11-27 2023-03-15 Syngenta Crop Protection Ag Composiciones plaguicidas
WO2022117650A1 (en) 2020-12-02 2022-06-09 Syngenta Crop Protection Ag Fungicidal compositions
UY39544A (es) 2020-12-02 2022-06-30 Syngenta Crop Protection Ag Composiciones fungicidas que comprenden una mezcla de componentes (a) y (b) como principios activos
MX2023006990A (es) 2020-12-14 2023-06-26 Basf Se Plaguicidas de sulfoximina.
EP3915971A1 (en) 2020-12-16 2021-12-01 Bayer Aktiengesellschaft Phenyl-s(o)n-phenylamidines and the use thereof as fungicides
WO2022129190A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft (hetero)aryl substituted 1,2,4-oxadiazoles as fungicides
MX2023007290A (es) 2020-12-18 2023-07-04 Bayer Ag Uso del inhibidor de dhodh para el control de hongos fitopatogenicos resistentes en cultivos.
WO2022129196A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft Heterobicycle substituted 1,2,4-oxadiazoles as fungicides
WO2022129188A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft 1,2,4-oxadiazol-3-yl pyrimidines as fungicides
EP4036083A1 (de) 2021-02-02 2022-08-03 Bayer Aktiengesellschaft 5-oxy substituierte hetereozyklen, als schädlingsbekämpfungsmittel
EP4043444A1 (en) 2021-02-11 2022-08-17 Basf Se Substituted isoxazoline derivatives
EP4291641A1 (en) 2021-02-11 2023-12-20 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin oxidase levels in plants
CA3211121A1 (en) 2021-02-25 2022-09-01 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
AR125089A1 (es) 2021-03-19 2023-06-07 Syngenta Crop Protection Ag Composiciones plaguicidas
BR112023019400A2 (pt) 2021-03-30 2023-12-05 Bayer Ag 3-(hetero)aril-5-clorodifluorometil-1,2,4-oxadiazol como fungicida
BR112023019788A2 (pt) 2021-03-30 2023-11-07 Bayer Ag 3-(hetero)aril-5-clorodifluorometil-1,2,4-oxadiazol como fungicida
WO2022233758A1 (en) 2021-05-03 2022-11-10 Basf Se Additives for enhancing the pesticidal effectiveness of pesticidal microorganisms
MX2023013014A (es) 2021-05-04 2023-11-15 Syngenta Crop Protection Ag Uso de cletodim para el control de insectos.
BR112023022763A2 (pt) 2021-05-06 2024-01-02 Bayer Ag Imidazóis anulados substituídos por alquilamida e uso dos mesmos como inseticidas
WO2022238391A1 (de) 2021-05-12 2022-11-17 Bayer Aktiengesellschaft 2-(het)aryl-substituierte kondensierte heterocyclen-derivate als schädlingsbekämpfungsmittel
EP4091451A1 (en) 2021-05-17 2022-11-23 BASF Agro B.V. Compositions comprising mefentrifluconazole
KR20240008856A (ko) 2021-05-18 2024-01-19 바스프 에스이 살진균제로서의 신규한 치환된 피리딘
EP4341256A1 (en) 2021-05-18 2024-03-27 Basf Se New substituted pyridines as fungicides
CA3219311A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted quinolines as fungicides
WO2022263285A1 (en) 2021-06-14 2022-12-22 Basf Se Yield improvement by gene combinations
CN117897050A (zh) 2021-06-17 2024-04-16 成对植物服务股份有限公司 大豆中生长调节因子家族转录因子的修饰
UY39827A (es) 2021-06-24 2023-01-31 Pairwise Plants Services Inc Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento
WO2023278651A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
AU2022301674A1 (en) 2021-07-02 2023-12-21 Syngenta Crop Protection Ag Use of fluazifop-p-butyl for insect control
EP4119547A1 (en) 2021-07-12 2023-01-18 Basf Se Triazole compounds for the control of invertebrate pests
CA3227665A1 (en) 2021-08-02 2023-02-09 Wassilios Grammenos (3-pirydyl)-quinazoline
CA3227653A1 (en) 2021-08-02 2023-02-09 Wassilios Grammenos (3-quinolyl)-quinazoline
US11771037B2 (en) 2021-08-10 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 08130343
US11678633B2 (en) 2021-08-10 2023-06-20 M.S. Technologies, L.L.C. Soybean cultivar 05330031
US11696551B2 (en) 2021-08-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 05320408
US11690343B2 (en) 2021-08-10 2023-07-04 M.S. Technologies, L.L.C. Soybean cultivar 02050022
US11659805B2 (en) 2021-08-10 2023-05-30 M.S. Technologies, L.L.C. Soybean cultivar 01160658
US11771036B2 (en) 2021-08-10 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 02050106
US11617332B2 (en) 2021-08-10 2023-04-04 M.S. Technologies, L.L.C. Soybean cultivar 03070412
US11632930B2 (en) 2021-08-10 2023-04-25 M.S. Technologies, L.L.C. Soybean cultivar 04290138
US11696554B2 (en) 2021-08-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 04190907
US11771038B2 (en) 2021-08-10 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 05140125
US11696550B2 (en) 2021-08-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 06130011
US11672228B2 (en) * 2021-08-10 2023-06-13 M.S. Technologies, L.L.C. Soybean cultivar 05150826
US11666030B2 (en) 2021-08-10 2023-06-06 M.S. Technologies, L.L.C. Soybean cultivar 08320812
US11696553B2 (en) 2021-08-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 02420133
US11690344B2 (en) 2021-08-10 2023-07-04 M.S. Technologies, L.L.C. Soybean cultivar 00250653
US11612129B2 (en) 2021-08-10 2023-03-28 M.S. Technologies, L.L.C. Soybean cultivar 06310200
US11647717B2 (en) 2021-08-10 2023-05-16 M.S. Technologies, L.L.C. Soybean cultivar 03420647
US20230056894A1 (en) * 2021-08-10 2023-02-23 M.S. Technologies, L.L.C. Soybean cultivar 02440128
US11696552B2 (en) 2021-08-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 06040447
US11612130B2 (en) 2021-08-11 2023-03-28 M.S. Technologies, L.L.C. Soybean cultivar 02430132
US11553679B1 (en) 2021-08-11 2023-01-17 M.S. Technologies, L.L.C. Soybean cultivar 06330352
US11553680B1 (en) 2021-08-11 2023-01-17 M.S. Technologies, L.L.C. Soybean cultivar 00220803
US11627715B2 (en) 2021-08-11 2023-04-18 M.S. Technologies, L.L.C. Soybean cultivar 04040309
US11707041B2 (en) 2021-08-11 2023-07-25 M.S. Technologies, L.L.C. Soybean cultivar 09110424
US11627716B2 (en) 2021-08-11 2023-04-18 M.S. Technologies, L.L.C. Soybean cultivar 04040632
US11553681B1 (en) 2021-08-11 2023-01-17 M.S. Technologies, L.L.C. Soybean cultivar 07040726
US11559027B1 (en) 2021-08-11 2023-01-24 M.S. Technologies, L.L.C. Soybean cultivar 07030206
CA3229056A1 (en) 2021-08-12 2023-02-16 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023017120A1 (en) 2021-08-13 2023-02-16 Bayer Aktiengesellschaft Active compound combinations and fungicide compositions comprising those
EP4388110A1 (en) 2021-08-17 2024-06-26 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin receptor histidine kinase genes in plants
EP4140986A1 (en) 2021-08-23 2023-03-01 Basf Se Pyrazine compounds for the control of invertebrate pests
US11647720B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 01130157
US11696556B2 (en) 2021-08-24 2023-07-11 Monsanto Technology, Llc Soybean cultivar 07080746
US11647724B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 03230124
US11678635B2 (en) 2021-08-24 2023-06-20 Stine Seed Farm, Inc. Soybean cultivar 01160922
US11647723B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 07060434
US11647721B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 02330517
US11678636B2 (en) 2021-08-24 2023-06-20 Monsanto Technology, Llc Soybean cultivar 04070428
US11666031B2 (en) 2021-08-24 2023-06-06 Monsanto Technology Llc Soybean cultivar 05140102
US11641838B2 (en) 2021-08-24 2023-05-09 Monsanto Technology Llc Soybean cultivar 04130514
US11647722B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 02140434
US11641837B2 (en) 2021-08-24 2023-05-09 Monsanto Technology Llc Soybean cultivar 02340134
WO2023025682A1 (en) 2021-08-25 2023-03-02 Bayer Aktiengesellschaft Novel pyrazinyl-triazole compounds as pesticides
EP4140995A1 (en) 2021-08-27 2023-03-01 Basf Se Pyrazine compounds for the control of invertebrate pests
US20230091662A1 (en) * 2021-08-30 2023-03-23 Monsanto Technology Llc Soybean cultivar 07180422
CA3230167A1 (en) 2021-08-30 2023-03-09 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
US11647725B2 (en) 2021-08-31 2023-05-16 Monsanto Technology Llc Soybean cultivar 07160416
US11700826B2 (en) 2021-08-31 2023-07-18 Monsanto Technology, Llc Soybean cultivar 02410315
US20230074685A1 (en) * 2021-08-31 2023-03-09 Monsanto Technology Llc Soybean cultivar 08110202
US11716947B2 (en) 2021-08-31 2023-08-08 Monsanto Technology, Llc Soybean cultivar 07030013
US11672230B2 (en) 2021-08-31 2023-06-13 Monsanto Technology, Llc Soybean cultivar 03260108
US11800847B2 (en) 2021-08-31 2023-10-31 Monsanto Technology, Llc Soybean cultivar 05050112
US11647727B2 (en) 2021-08-31 2023-05-16 Monsanto Technology Llc Soybean cultivar 04120446
US11647726B2 (en) 2021-08-31 2023-05-16 Monsanto Technology Llc Soybean cultivar 04070902
US11672229B2 (en) 2021-08-31 2023-06-13 Monsanto Technology, Llc Soybean cultivar 08180710
US11856914B2 (en) 2021-08-31 2024-01-02 Monsanto Technology, Llc Soybean cultivar 01140108
US11696558B2 (en) 2021-08-31 2023-07-11 Monsanto Technology, Llc Soybean cultivar 02021411
US11700827B2 (en) 2021-08-31 2023-07-18 Monsanto Technology, Llc Soybean cultivar 07220837
US20230068454A1 (en) * 2021-08-31 2023-03-02 Monsanto Technology Llc Soybean cultivar 05020906
US11696557B2 (en) 2021-08-31 2023-07-11 Monsanto Technology, Llc Soybean cultivar 04060955
US11700825B2 (en) 2021-08-31 2023-07-18 Monsanto Technology, Llc Soybean cultivar 06170324
EP4144739A1 (de) 2021-09-02 2023-03-08 Bayer Aktiengesellschaft Anellierte pyrazole als schädlingsbekämpfungsmittel
AR126938A1 (es) 2021-09-02 2023-11-29 Pairwise Plants Services Inc Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento
EP4151631A1 (en) 2021-09-20 2023-03-22 Basf Se Heterocyclic compounds for the control of invertebrate pests
US20230087522A1 (en) 2021-09-21 2023-03-23 Pairwise Plants Services, Inc. Methods and compositions for reducing pod shatter in canola
US20230102442A1 (en) * 2021-09-28 2023-03-30 Monsanto Technology Llc Soybean variety 01091553
US20230102917A1 (en) * 2021-09-28 2023-03-30 Monsanto Technology Llc Soybean variety 01083685
US11985949B2 (en) * 2021-09-28 2024-05-21 Monsanto Technology Llc Soybean variety 01091555
US11917968B2 (en) 2021-09-28 2024-03-05 Monsanto Technology Llc Soybean variety 01083684
US20230106090A1 (en) * 2021-09-28 2023-04-06 Monsanto Technology Llc Soybean variety 01091552
US20230103176A1 (en) * 2021-09-28 2023-03-30 Monsanto Technology Llc Soybean variety 01083675
US11744217B2 (en) 2021-09-29 2023-09-05 Monsanto Technology, Llc Soybean variety 01091711
US20230101351A1 (en) * 2021-09-29 2023-03-30 Monsanto Technology Llc Soybean variety 01091573
US11744216B2 (en) 2021-09-29 2023-09-05 Monsanto Technology, Llc Soybean variety 01091693
US11917970B2 (en) 2021-09-29 2024-03-05 Monsanto Technology Llc Soybean variety 01091697
US11917971B2 (en) 2021-09-29 2024-03-05 Monsanto Technology Llc Soybean variety 01091703
US11716958B2 (en) 2021-09-29 2023-08-08 Monsanto Technology, Llc Soybean variety 01091691
US20230116212A1 (en) * 2021-09-29 2023-04-13 Monsanto Technology Llc Soybean variety 01091606
US11758869B2 (en) 2021-09-29 2023-09-19 M.S. Technologies, L.L.C. Soybean variety 01091607
US20230098816A1 (en) * 2021-09-29 2023-03-30 Monsanto Technology Llc Soybean variety 01091694
US11930768B2 (en) 2021-09-29 2024-03-19 Monsanto Technology Llc Soybean variety 01091702
US11716957B2 (en) 2021-09-29 2023-08-08 Monsanto Technology, Llc Soybean variety 01091574
US11839192B2 (en) 2021-09-29 2023-12-12 Monsanto Technology, Llc Soybean variety 01091705
US11925161B2 (en) 2021-09-29 2024-03-12 Monsanto Technology Llc Soybean variety 01091628
US11744218B2 (en) 2021-09-30 2023-09-05 Monsanto Technology, Llc Soybean variety 01091749
US11751529B2 (en) 2021-09-30 2023-09-12 M.S. Technologies, L.L.C. Soybean variety 01091734
US12004475B2 (en) 2021-09-30 2024-06-11 Monsanto Technology Llc Soybean variety 01091767
US20230101646A1 (en) * 2021-09-30 2023-03-30 Monsanto Technology Llc Soybean variety 01091722
US12004474B2 (en) 2021-09-30 2024-06-11 Monsanto Technology Llc Soybean variety 01091721
US20230096311A1 (en) * 2021-09-30 2023-03-30 Monsanto Technology Llc Soybean variety 01091714
US20230094163A1 (en) * 2021-09-30 2023-03-30 Monsanto Technology Llc Soybean variety 01091757
US11930769B2 (en) 2021-09-30 2024-03-19 Monsanto Technology Llc Soybean variety 01091763
US20230100506A1 (en) * 2021-09-30 2023-03-30 Monsanto Technology Llc Soybean variety 01091758
US20230103672A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091739
US20230104138A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091783
US11917973B2 (en) 2021-10-01 2024-03-05 Monsanto Technology Llc Soybean variety 01091785
US20230104140A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091788
US11930770B2 (en) 2021-10-01 2024-03-19 Monsanto Technology Llc Soybean variety 01091765
US11917972B2 (en) 2021-10-01 2024-03-05 Monsanto Technology Llc Soybean variety 01091777
US20230107487A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091791
US20230105612A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091790
US20230108697A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091782
US11963509B2 (en) 2021-10-01 2024-04-23 Monsanto Technology Llc Soybean variety 01091775
US20230103384A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091772
US20230107232A1 (en) * 2021-10-04 2023-04-06 Monsanto Technology Llc Soybean variety 01091804
US20230108968A1 (en) 2021-10-04 2023-04-06 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
US11832578B2 (en) 2021-10-04 2023-12-05 Monsanto Technology, Llc Soybean variety 01091795
US11839193B2 (en) 2021-10-04 2023-12-12 Monsanto Technology, Llc Soybean variety 01091798
US20230107224A1 (en) * 2021-10-04 2023-04-06 Monsanto Technology Llc Soybean variety 01091811
US11917974B2 (en) 2021-10-04 2024-03-05 Monsanto Technology, Llc Soybean variety 01091792
US20230107769A1 (en) * 2021-10-04 2023-04-06 Monsanto Technology Llc Soybean variety 01091810
US11903358B2 (en) 2021-10-04 2024-02-20 Monsanto Technology Llc Soybean variety 01091805
CA3234455A1 (en) 2021-10-07 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023072670A1 (en) 2021-10-28 2023-05-04 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors x
WO2023072671A1 (en) 2021-10-28 2023-05-04 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ix
WO2023078915A1 (en) 2021-11-03 2023-05-11 Bayer Aktiengesellschaft Bis(hetero)aryl thioether (thio)amides as fungicidal compounds
US12010971B2 (en) 2021-11-19 2024-06-18 M.S. Technologies, L.L.C. Soybean cultivar 05170605
WO2023099445A1 (en) 2021-11-30 2023-06-08 Bayer Aktiengesellschaft Bis(hetero)aryl thioether oxadiazines as fungicidal compounds
EP4194453A1 (en) 2021-12-08 2023-06-14 Basf Se Pyrazine compounds for the control of invertebrate pests
AR127904A1 (es) 2021-12-09 2024-03-06 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
EP4198033A1 (en) 2021-12-14 2023-06-21 Basf Se Heterocyclic compounds for the control of invertebrate pests
EP4198023A1 (en) 2021-12-16 2023-06-21 Basf Se Pesticidally active thiosemicarbazone compounds
AR128372A1 (es) 2022-01-31 2024-04-24 Pairwise Plants Services Inc Supresión de la respuesta de evitación de la sombra en las plantas
WO2023148036A1 (en) 2022-02-01 2023-08-10 Globachem Nv Methods and compositions for controlling pests in soybean
WO2023148028A1 (en) 2022-02-01 2023-08-10 Globachem Nv Methods and compositions for controlling pests
WO2023156402A1 (en) 2022-02-17 2023-08-24 Basf Se Pesticidally active thiosemicarbazone compounds
WO2023156270A1 (en) 2022-02-18 2023-08-24 Basf Se Coumarin synthesis and uses thereof
WO2023168217A1 (en) 2022-03-02 2023-09-07 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
EP4238971A1 (en) 2022-03-02 2023-09-06 Basf Se Substituted isoxazoline derivatives
WO2023192838A1 (en) 2022-03-31 2023-10-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
US20230357789A1 (en) 2022-04-07 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for improving resistance to fusarium head blight
WO2023205714A1 (en) 2022-04-21 2023-10-26 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2023215704A1 (en) 2022-05-02 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for enhancing yield and disease resistance
WO2023213626A1 (en) 2022-05-03 2023-11-09 Bayer Aktiengesellschaft Use of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine for controlling unwanted microorganisms
WO2023213670A1 (en) 2022-05-03 2023-11-09 Bayer Aktiengesellschaft Crystalline forms of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine
WO2023215809A1 (en) 2022-05-05 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture and/or improving plant yield traits
WO2024006679A1 (en) 2022-06-27 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
US20240002873A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
US20240000031A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024018016A1 (en) 2022-07-21 2024-01-25 Syngenta Crop Protection Ag Crystalline forms of 1,2,4-oxadiazole fungicides
WO2024028243A1 (en) 2022-08-02 2024-02-08 Basf Se Pyrazolo pesticidal compounds
US20240043857A1 (en) 2022-08-04 2024-02-08 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
US20240060081A1 (en) 2022-08-11 2024-02-22 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024033374A1 (en) 2022-08-11 2024-02-15 Syngenta Crop Protection Ag Novel arylcarboxamide or arylthioamide compounds
WO2024054880A1 (en) 2022-09-08 2024-03-14 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
EP4342885A1 (en) 2022-09-20 2024-03-27 Basf Se N-(3-(aminomethyl)-phenyl)-5-(4-phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-amine derivatives and similar compounds as pesticides
GB202214203D0 (en) 2022-09-28 2022-11-09 Syngenta Crop Protection Ag Fungicidal compositions
WO2024068519A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068517A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
EP4295688A1 (en) 2022-09-28 2023-12-27 Bayer Aktiengesellschaft Active compound combination
GB202214202D0 (en) 2022-09-28 2022-11-09 Syngenta Crop Protection Ag Agricultural methods
WO2024068518A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-heteroaryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068520A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
EP4361126A1 (en) 2022-10-24 2024-05-01 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors xv
WO2024100069A1 (en) 2022-11-08 2024-05-16 Syngenta Crop Protection Ag Microbiocidal pyridine derivatives
WO2024104822A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted tetrahydrobenzodiazepine as fungicides
WO2024104815A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted benzodiazepines as fungicides
WO2024104823A1 (en) 2022-11-16 2024-05-23 Basf Se New substituted tetrahydrobenzoxazepine
WO2024104818A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted benzodiazepines as fungicides
EP4385326A1 (en) 2022-12-15 2024-06-19 Kimitec Biogorup Biopesticide composition and method for controlling and treating broad spectrum of pests and diseases in plants
EP4389210A1 (en) 2022-12-21 2024-06-26 Basf Se Heteroaryl compounds for the control of invertebrate pests

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849482A (en) * 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5659113A (en) * 1995-12-04 1997-08-19 Asgrow Seed Company Soybean cultivar A5547
US5824850A (en) 1997-02-07 1998-10-20 Asgrow Seed Company Soybean cultivar 8816079010574
US6177617B1 (en) 1997-02-07 2001-01-23 Asgrow Seed Company Soybean cultivar 89248009206
US6376754B1 (en) 1997-03-07 2002-04-23 Asgrow Seed Company Plants having resistance to multiple herbicides and its use
US7368527B2 (en) * 1999-03-12 2008-05-06 Human Genome Sciences, Inc. HADDE71 polypeptides
US20040031072A1 (en) * 1999-05-06 2004-02-12 La Rosa Thomas J. Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
food derived from glufosinate ammonium tolerant soybean lines A2704-12 and A5547-127;a safeth assessment;《food standards australia new zealand》;20050131;1-37

Also Published As

Publication number Publication date
US20080196127A1 (en) 2008-08-14
EP1871901A2 (en) 2008-01-02
PL1871901T3 (pl) 2011-11-30
BRPI0610462A2 (pt) 2010-06-22
PT1871901E (pt) 2011-09-29
SI1871901T1 (sl) 2011-10-28
US20120117693A1 (en) 2012-05-10
ES2369031T3 (es) 2011-11-24
DK1871901T3 (da) 2011-10-17
US8952142B2 (en) 2015-02-10
WO2006108675A2 (en) 2006-10-19
EP1871901B1 (en) 2011-06-29
CA2603949A1 (en) 2006-10-19
JP5281392B2 (ja) 2013-09-04
US8017756B2 (en) 2011-09-13
CN101155933B (zh) 2015-09-16
JP2008535509A (ja) 2008-09-04
CA2603949C (en) 2014-12-09
MX2007012561A (es) 2008-02-22
ES2369032T3 (es) 2011-11-24
CN105112520A (zh) 2015-12-02
ATE514792T1 (de) 2011-07-15
WO2006108675A3 (en) 2007-01-11
CN101155933A (zh) 2008-04-02
BRPI0610462B1 (pt) 2018-02-27

Similar Documents

Publication Publication Date Title
CN105112520B (zh) 原种事件a5547-127以及在生物样品中鉴定此类事件的方法和试剂盒
CN101151373B (zh) 原种事件a2704-12以及用于鉴定生物样品中此事件的方法和试剂盒
US6951929B2 (en) Methods and kits for identifying elite event GAT-ZM1 in biological samples
CN101238217B (zh) 耐受除草剂的棉花植物和用于鉴定其的方法
JP5762400B2 (ja) イネ遺伝子組換え事象１７０５３およびその使用方法
US8313909B2 (en) Herbicide tolerant rice plants and methods for identifying same
JP5769698B2 (ja) 遺伝子組換えイネ事象１７３１４およびその使用方法
AU2001235414A1 (en) Methods and kits for identifying elite event GAT-ZM1 in biological samples
CN112094940B (zh) 与黄瓜长下胚轴基因lh1连锁的Indel标记及其引物、试剂盒与应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20200323

Address after: new jersey

Patentee after: Bayer CropScience L.P.

Address before: Diheim, Belgium

Patentee before: Bayer CropScience N.V.

TR01 Transfer of patent right