CN105112308A - Gibberella moniliformis and application of gibberella moniliformis to growth promotion and drought resistance for dendrobium officinale - Google Patents
Gibberella moniliformis and application of gibberella moniliformis to growth promotion and drought resistance for dendrobium officinale Download PDFInfo
- Publication number
- CN105112308A CN105112308A CN201510623045.9A CN201510623045A CN105112308A CN 105112308 A CN105112308 A CN 105112308A CN 201510623045 A CN201510623045 A CN 201510623045A CN 105112308 A CN105112308 A CN 105112308A
- Authority
- CN
- China
- Prior art keywords
- beading
- herba dendrobii
- jsnl003
- gibberella moniliformis
- gibberellamoniliformis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses gibberella moniliformis. The classification name of the gibberella moniliformis is gibberella moniliformis of gibberella of ascomycotina, a strain number of the gibberella moniliformis is JSNL003, the gibberella moniliformis is preserved in the Common Microorganism Center of the China Committee of Culture Collection of Microogranisms on September 11, 2015, and a preservation number of the gibberella moniliformis is CGMCC No.11309. The gibberella moniliformis has the advantages that obvious effects can be realized by the gibberella moniliformis JSNL003 in the aspects of increasing the growth rate of dendrobium officinale, improving the drought resistance of the dendrobium officinale and the like; the gibberella moniliformis can be artificially cultivated, is high in reproduction speed and convenient to manufacture on a large scale and has excellent development and application prospects.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to the red mould and application in Herba Dendrobii growth-promoting, drought resisting of a kind of beading.
Background technology
Herba Dendrobii is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, the effect such as have immunomodulatory, delay senility, and being the very rare rare traditional Chinese medicine recorded in China's pharmacopeia, is the required raw material element of some Chinese patent medicines, healthcare products.Mycorhiza is the symbiote with specific modality structure and function that in soil, mycorrhizal fungi and root system of plant are formed, and is the ubiquitous a kind of commensalism of occurring in nature.Orchid is comparatively strong to the dependency of mycorhiza, mycorrhizal fungi and the conditional formation symbiote of root system of plant, physiology is the entirety mutually coordinated, and in the process of common evolutionary, forms specific morphological specificity.Because orchid and fungi have natural symbiotic relationship, from its seed germination to the healthy growth of seedling, invariably need the participation of fungi, this makes the artificial culture of Herba Dendrobii comparatively difficult, and the artificial growth speed of growth is slow, and mean yield is low, disease occurs comparatively serious.At present, demand, mainly by excavating wild resource, causes dendrobium officinale resource to be on the verge of exhaustion, is classified as the medicinal plant of two classes focused protections by country.The mutual relationship of mycorrhizal fungi and host plant is in most cases mutually beneficial, and mycorrhizal fungi obtains the required nutritive substance of growth in plant materials, and plant is by being combined with symbiotic effects, also can better obtain required nutrient and moisture etc. from soil.In recent years, have many scholars to carry out large quantity research the diversity of the mycorhiza structure of Dendrobium Sw, mycorrhizal fungi, mycorrhizal fungi in the Effect study of Dendrobium Sw etc., the high-efficiency artificial cultivation carrying out Herba Dendrobii for the good characteristic making full use of mycorhiza is laid a good foundation.
Mycorrhizal fungi has certain effect to growing of plant.After artificial inoculation Epulorhiza (Epulorhiza) bacterial strain 60d is carried out to the tissue culturing seedling of Herba Dendrobii, connect vaccine mean fresh rate of increase and gone out 84.8% than contrast height of seedling; After candidum tissue culturing seedling inoculation Orchidaceous rhizoctonias Cymbidiumfaberikanran and Cymbidiumsinense, mean fresh rate of increase all reaches the level of pole significant difference, and the tissue cultured seedling of inoculation Cymbidiumgoeringii and Cymbidiumgoeringii reaches the level of significant difference; The endogenetic fungus of Mycena can promote growing of Herba Dendrobii seedling; After isolated GN09, GN10 and GN43 bacterial strain of pleione bulbocodioides (Pleionebulbocodioides) and dendrobium loddigesii Rolfe (DendrobiumloddigesiiRolfe) tissue cultured seedling symbiosis culture, the fresh weight rate of increase of tissue cultured seedling improves 69.8%, 61.9% and 81.7% than contrast respectively, has great using value significantly.From preserving the ecological environment and the angle of food safety, Herba Dendrobii is as rare Chinese medicine, and the amount of application of chemical fertilizer as far as possible, can improve product quality greatly, reaches the object of safe edible, and this also meets the strategy of sustainable development of agricultural.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of to Herba Dendrobii growth-promoting, degeneration-resistant action effect mycorrhizal fungi significantly---Ascomycotina Gibberella beading red mould (Gibberellamoniliformis).
The technical problem that the present invention also will solve is to provide above-mentioned fungi and is improving the application in Growth of Dendrobium candidum speed, drought-resistant ability.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of beading are red mould, its Classification And Nomenclature is Ascomycotina Gibberella beading red mould (Gibberellamoniliformis), bacterial strain number is JSNL003, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number is CGMCCNo.11309, and preservation date is on September 11st, 2015.Preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode 100101.
Above-mentioned bacterial strains JSNL003 the good plant of upgrowth situation is separated to obtain in August, 2012 in Jurong City of Jiangsu Province Herba Dendrobii plants district, and this bacterial strain has significant growth-promoting functions to Herba Dendrobii, also obviously can improve the drought-resistant ability of Herba Dendrobii.
The colony characteristics of beading red mould (Gibberellamoniliformis) JSNL003 is as follows:
Morphological observation shows: cultivate on PDA flat board, mycelia canescence, and loose, bacterium colony is uneven, and tow sides are canescence, the full edge of colony edge.Amplification ITS district complete sequence also checks order, and the 16sRNA complete sequence that pcr amplification obtains is as shown in SEQIDNo:1.
The red mould application in promotion Growth of Dendrobium candidum of above-mentioned beading is also within protection scope of the present invention.
Application in the red mould drought-resistant ability improving Herba Dendrobii of above-mentioned beading is also within protection scope of the present invention.
Concrete application method is: red for beading mould JSNL003 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
Wherein, described solid fungicide prepares as follows:
With cotton seed hulls bacterium slag be culture medium, ammonium sulfate is external source nitrogenous source, glucose is for external source carbon source, be prepared into solid medium, sterilizing, beading red mould (Gibberellamoniliformis) JSNL003 is inoculated under aseptic condition, 27 DEG C, cultivate 20 ~ 25 days under dark, the pH6.5 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) beading red mould (Gibberellamoniliformis) JSNL003 to the growth-promoting of Herba Dendrobii and degeneration-resistant action effect all comparatively remarkable, having certain effect to the use reducing chemical fertilizer and agricultural chemicals, is also the important means ensureing edible safety.
(2) beading red mould (Gibberellamoniliformis) JSNL003 culture condition simply, is easily preserved, and is easy to suitability for industrialized production, has good development prospect.
Accompanying drawing explanation
Fig. 1 is that JSNL003 bacterium colony cultivates photo.
Fig. 2 is the growth-promoting effect of JSNL003 to Herba Dendrobii plant.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
Beading red mould (Gibberellamoniliformis) JSNL003 analyzes the growth-promoting of Herba Dendrobii and drought-resistant ability action effect.
Red for mycorrhizal fungi beading mould (Gibberellamoniliformis) JSNL003 is prepared into solid fungicide.Concrete preparation method is: with cotton seed hulls bacterium slag be culture medium, ammonium sulfate is external source nitrogenous source, glucose for external source carbon source, be prepared into solid culture matrix and load in autoclaving bag, sterilizing 20 minutes under the condition of high voltage of 121 DEG C and 101.3 kPas.Aseptically Arbuscular Mycorrhizal Fungi fungi JSNL003 after culture medium cooling, then carries out artificial culture.Concrete culture condition is 27 DEG C, 12h illumination+12h is dark, pH6.5, cultivates 20 ~ 25 days.Cultured microbial inoculum is processed into 1cm
3the block of size, is positioned over the Herba Dendrobii seedling cultivation matrix surface of planting then.Cultivation of dendrobium officinale selects the nutrition pot of bore 15cm, and each nutrition pot cultivates 1 clump of (5 strain) Herba Dendrobii.Select the Herba Dendrobii plant that growing way is basically identical during inoculation, and carry out the mensuration of height of seedling and leading thread.The solid fungicide inoculum size of every clump of seedlings of Dendrobium officinale is 5g.After using microbial inoculum, Relative water content maintains more than 60% 10 day, guarantees that mycelia survives in the substrate.Inoculate latter 6 months, carry out the Mycorrhizal degree detecting of Herba Dendrobii root system, guarantee that mycorrhizal fungi beading red mould (Gibberellamoniliformis) JSNL003 inoculated is combined with Herba Dendrobii root, define mycorhiza.Inoculate latter 12 months, carry out growth-promoting and the drought resisting effect analysis (table 1, table 2) of Herba Dendrobii Mycorrhizal plant.With non-Arbuscular Mycorrhizal Fungi microbial inoculum, the Herba Dendrobii plant only inoculating equivalent cotton seed hulls bacterium slag is adjoining tree (CK).
The drought stress process of Herba Dendrobii plant: the Mycorrhizal Herba Dendrobii choosing different treatment, unification is watered, the soil moisture content of every basin plant is reached capacity, then coerce in warm lab simulation natural drought, moisture is constantly consumed, is coercing 10d, 20d, 30d and carry out the mensuration of wilting degree, wilted percent.Temperature room temp is 30-35 DEG C, and relative air humidity is 50-60%.Often kind of process 60 strains, do 3 repetitions.
Under drought stress conditions, according to following standard, the degree of suffering from drought of each process Herba Dendrobii is divided into 5 grades: 0: healthy; I: wilting appears in minority blade; II: wilting appears in half blade; III: blade more than half is wilted; IV: whole strain is wilted.Add up wilted percent and the wilting degree of each process plant.
Wilted percent (%)=(occurring wilting symptom strain number/process strain number) × 100%
Wilting degree=[∑ (wilting progression × strain number) × 100]/(highest number of wilting × total strain number)
Table 1JSNL003 is to the growth-promoting effect of Herba Dendrobii plant
Table 2JSNL003 is to the action effect of Herba Dendrobii plant drought-resistant ability
Claims (7)
1. beading are red mould, its Classification And Nomenclature is Ascomycotina Gibberella beading red mould (Gibberellamoniliformis), bacterial strain number is JSNL003, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCCNo.11309, and preservation date is on September 11st, 2015.
2. the red mould application in promotion Growth of Dendrobium candidum of beading according to claim 1.
3. application according to claim 2, it is characterized in that, red for beading mould (Gibberellamoniliformis) JSNL003 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
4. application according to claim 3, it is characterized in that, described solid fungicide prepares as follows: with cotton seed hulls bacterium slag be culture medium, ammonium sulfate is external source nitrogenous source, glucose is for external source carbon source, be prepared into solid medium, sterilizing, inoculate beading red mould (Gibberellamoniliformis) JSNL003 under aseptic condition, 27 DEG C, cultivate 20 ~ 25 days under dark, the pH6.5 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
5. the application in the red mould drought-resistant ability improving Herba Dendrobii of beading according to claim 1.
6. application according to claim 5, it is characterized in that, red for beading mould (Gibberellamoniliformis) JSNL003 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
7. application according to claim 6, it is characterized in that, described solid fungicide prepares as follows: with cotton seed hulls bacterium slag be culture medium, ammonium sulfate is external source nitrogenous source, glucose is for external source carbon source, be prepared into solid medium, sterilizing, inoculate beading red mould (Gibberellamoniliformis) JSNL003 under aseptic condition, 27 DEG C, cultivate 20 ~ 25 days under dark, the pH6.5 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510623045.9A CN105112308B (en) | 2015-09-25 | 2015-09-25 | An a kind of beading are red mould and its in dendrobium candidum growth-promoting and the application in drought resisting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510623045.9A CN105112308B (en) | 2015-09-25 | 2015-09-25 | An a kind of beading are red mould and its in dendrobium candidum growth-promoting and the application in drought resisting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105112308A true CN105112308A (en) | 2015-12-02 |
| CN105112308B CN105112308B (en) | 2018-03-09 |
Family
ID=54660426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510623045.9A Active CN105112308B (en) | 2015-09-25 | 2015-09-25 | An a kind of beading are red mould and its in dendrobium candidum growth-promoting and the application in drought resisting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105112308B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114507618A (en) * | 2022-01-11 | 2022-05-17 | 云南大学 | Turkey mycorrhiza strain TP-11 with capacity of promoting growth of new leaves of dendrobium and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
-
2015
- 2015-09-25 CN CN201510623045.9A patent/CN105112308B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
Non-Patent Citations (2)
| Title |
|---|
| 郭顺星等: "铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定", 《中国中药杂志》 * |
| 陈连庆 等: "石斛气生的兰科菌根组织结构及其对御旱研究", 《生态环境学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114507618A (en) * | 2022-01-11 | 2022-05-17 | 云南大学 | Turkey mycorrhiza strain TP-11 with capacity of promoting growth of new leaves of dendrobium and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105112308B (en) | 2018-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103214302B (en) | Sawdust mushroom residue nutrition agent used for cultivating mozzie buster, and preparing method thereof | |
| CN104195055B (en) | A kind of Fusarium oxysporum and the application in Herba Dendrobii disease-resistanting leaf spot thereof | |
| CN103992184B (en) | A kind of biological organic fertilizer | |
| CN104195056B (en) | A kind of two type umbrellas are mould and application in Herba Dendrobii growth-promoting, drought resisting | |
| CN105219656B (en) | A kind of Fusarium oxysporum and its application in Growth of Dendrobium candidum is promoted | |
| CN107475134B (en) | One plant of Fusarium solani and its application in prevention and treatment dendrobium candidum southern blight | |
| CN103588544B (en) | A kind of microbial organic fertilizer and its production and use | |
| CN105132293B (en) | A kind of Alternaria tenuissima and its application in dendrobium candidum growth-promoting, drought resisting | |
| CN103382139A (en) | Agaricus bisporus (Lange) Sing culture medium | |
| CN103145497B (en) | Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof | |
| CN104195054B (en) | Mould and the application in dendrobium candidum growth-promoting and drought resisting of a kind of two type umbrellas | |
| CN103283608B (en) | Factory cultivation strains of needle mushrooms, and cultivation method thereof | |
| CN108401794A (en) | A kind of armillaria mellea accreting with Rhizoma Gastrodiae liquid spawn production method and cultigen special culture media | |
| CN104478546A (en) | Method for utilizing vitex negundo var ineica scrap to culture hericium erinaceus | |
| CN101914456A (en) | Fungus for promoting orchid seeds to germinate | |
| CN105132294B (en) | A kind of alternaric bacteria and its application in dendrobium candidum disease-resistanting leaf spot | |
| CN107778035A (en) | A kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof | |
| CN105724055B (en) | A method of improving agaricus bisporus yield using needle mushroom dreg | |
| CN106978358B (en) | One plant of Fusarium oxysporum for promoting Growth of Dendrobium candidum and its application | |
| CN107641600B (en) | Pleurotus ostreatus JK02 strain suitable for low-temperature fruiting and cultivation method and application thereof | |
| CN104478547B (en) | A kind of method of the yellow umbrella of utilization twigs of the chaste tree bits culture | |
| CN106978359B (en) | One plant of Fusarium solani and its application in the anti-root rot of dendrobium candidum | |
| CN106967624B (en) | One plant of Fusarium oxysporum and its application in officinal dendrobium stem plantation | |
| CN101914457B (en) | Fungus for promoting germination of bletilla striata seeds | |
| CN103540534B (en) | The industrial method for culturing of high-protein desert algae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |